Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95358.t1 | AES94978.2 | 94.8 | 425 | 18 | 2 | 1 | 425 | 1 | 421 | 1.80E-196 | 695.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95358.t1 | O82653 | 60.4 | 439 | 153 | 7 | 2 | 425 | 5 | 437 | 1.2e-132 | 474.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95358.t1 | G7KDM7 | 94.8 | 425 | 18 | 2 | 1 | 425 | 1 | 421 | 1.3e-196 | 695.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059935 | MS.gene95358 | 0.81799 | 2.48E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95358.t1 | MTR_5g020440 | 96.471 | 425 | 11 | 2 | 1 | 425 | 1 | 421 | 0.0 | 834 |
| MS.gene95358.t1 | MTR_6g024095 | 95.529 | 425 | 11 | 3 | 1 | 425 | 1 | 417 | 0.0 | 818 |
| MS.gene95358.t1 | MTR_1g033240 | 81.163 | 430 | 37 | 7 | 1 | 425 | 1 | 391 | 0.0 | 665 |
| MS.gene95358.t1 | MTR_8g077125 | 26.159 | 302 | 215 | 4 | 124 | 425 | 101 | 394 | 6.73e-25 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95358.t1 | AT1G30960 | 63.883 | 443 | 133 | 7 | 1 | 425 | 4 | 437 | 0.0 | 514 |
| MS.gene95358.t1 | AT5G66470 | 21.957 | 419 | 306 | 8 | 13 | 425 | 2 | 405 | 3.61e-25 | 107 |
| MS.gene95358.t1 | AT5G66470 | 21.957 | 419 | 306 | 8 | 13 | 425 | 2 | 405 | 3.61e-25 | 107 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGTCTGTTGGGATTAT+TGG | 0.163623 | 1.3:-18340172 | MS.gene95358:CDS |
| TTTAGGCGTTTCATGATATC+TGG | 0.252865 | 1.3:-18334298 | MS.gene95358:intron |
| ACTTCCTCGGTCATGGTAAA+TGG | 0.262332 | 1.3:+18333936 | None:intergenic |
| GAAGGTTGCGTCTGAATGTT+CGG | 0.298343 | 1.3:+18340468 | None:intergenic |
| GCAAGTGCATCTAGTTCTTC+AGG | 0.309721 | 1.3:-18333172 | MS.gene95358:CDS |
| GAAGGGGAGAATGAAGTTTA+AGG | 0.311054 | 1.3:-18340323 | MS.gene95358:CDS |
| CACTTTCTCACTAACAAATT+AGG | 0.329869 | 1.3:-18333673 | MS.gene95358:CDS |
| AGTTGCGAGATGGTTCTCTT+AGG | 0.351624 | 1.3:-18333705 | MS.gene95358:CDS |
| GAAGAATTCGAAGATCTTCC+TGG | 0.354726 | 1.3:-18334453 | MS.gene95358:CDS |
| TTCTTATTCCACGTCACCTT+CGG | 0.364738 | 1.3:+18340388 | None:intergenic |
| TTTACCTGGTGATATGTCTA+TGG | 0.367841 | 1.3:+18336889 | None:intergenic |
| ATCAGAAGTCTTTGTCTGTT+GGG | 0.373365 | 1.3:-18340180 | MS.gene95358:CDS |
| AGATTTGACTCAGTTCTTGA+TGG | 0.373919 | 1.3:-18334254 | MS.gene95358:CDS |
| GAATGTTCGGGTTGAGGATC+GGG | 0.385005 | 1.3:+18340481 | None:intergenic |
| TGTTTGTTTAACAGGAGAAT+AGG | 0.397704 | 1.3:-18333234 | MS.gene95358:intron |
| TCGGGTTGAGGATCGGGTTC+CGG | 0.405609 | 1.3:+18340487 | None:intergenic |
| GGCGCTTGAGAAGGCTTTGT+TGG | 0.406248 | 1.3:-18340275 | MS.gene95358:CDS |
| AAGGTTGCGTCTGAATGTTC+GGG | 0.410075 | 1.3:+18340469 | None:intergenic |
| TGTTGTTGTTGTTGAGAATT+GGG | 0.414155 | 1.3:+18340425 | None:intergenic |
| AGCCAAGTTACTCACAATTT+GGG | 0.415515 | 1.3:+18337993 | None:intergenic |
| TGAATGTTCGGGTTGAGGAT+CGG | 0.418854 | 1.3:+18340480 | None:intergenic |
| GGGTTCCGGTGTGAGGGGTT+CGG | 0.422928 | 1.3:+18340501 | None:intergenic |
| CGAGCCTTGGCATCCTTATG+AGG | 0.427042 | 1.3:+18336972 | None:intergenic |
| CAAGTTTATCCGTCCGGTCT+CGG | 0.427824 | 1.3:+18340362 | None:intergenic |
| GCGTCTGAATGTTCGGGTTG+AGG | 0.430265 | 1.3:+18340475 | None:intergenic |
| TTAAACTTCATTCTCCCCTT+CGG | 0.438932 | 1.3:+18340325 | None:intergenic |
| AGGATCGGGTTCCGGTGTGA+GGG | 0.442223 | 1.3:+18340495 | None:intergenic |
| AATGAAGTTTAAGGAGGAAG+AGG | 0.447638 | 1.3:-18340314 | MS.gene95358:CDS |
| GGCCAACTCAAGATTCTTGT+GGG | 0.471826 | 1.3:-18333652 | MS.gene95358:CDS |
| GAGGATCGGGTTCCGGTGTG+AGG | 0.472229 | 1.3:+18340494 | None:intergenic |
| AGTTGTGCGTGAAAGGTTGC+TGG | 0.472622 | 1.3:-18333892 | MS.gene95358:CDS |
| AGCACTTTCAACACGAGCCT+TGG | 0.476228 | 1.3:+18336959 | None:intergenic |
| TTTCATGATATCTGGAATGA+AGG | 0.478892 | 1.3:-18334290 | MS.gene95358:CDS |
| TAAACCCGAGGAGGAGGAGG+AGG | 0.480366 | 1.3:-18340239 | MS.gene95358:CDS |
| ATTATTGGTGCTCCTAATGC+TGG | 0.481153 | 1.3:-18340157 | MS.gene95358:CDS |
| GAAGATTGTGGTGGTGGTGG+TGG | 0.482844 | 1.3:+18340540 | None:intergenic |
| AATAGGCAGTAGAACGGCCC+TGG | 0.484531 | 1.3:-18333968 | MS.gene95358:intron |
| GACTCAGTTCTTGATGGAGC+AGG | 0.486458 | 1.3:-18334248 | MS.gene95358:intron |
| CATTAGAAGTTGTGCGTGAA+AGG | 0.488172 | 1.3:-18333899 | MS.gene95358:CDS |
| GAGTAAACCCGAGGAGGAGG+AGG | 0.490946 | 1.3:-18340242 | MS.gene95358:CDS |
| AGACCTTACCTTTCAAATCC+AGG | 0.490953 | 1.3:+18334435 | None:intergenic |
| GATCAGAAGTCTTTGTCTGT+TGG | 0.496354 | 1.3:-18340181 | MS.gene95358:CDS |
| TTTCATAACTTCCTCGGTCA+TGG | 0.497634 | 1.3:+18333929 | None:intergenic |
| AACCTATGTTCAATATCGTA+TGG | 0.502441 | 1.3:+18333742 | None:intergenic |
| CACCACAACTCATGAAGTTG+TGG | 0.507926 | 1.3:-18338037 | MS.gene95358:CDS |
| TTGTAGCGGATTCCCTCATA+AGG | 0.509442 | 1.3:-18336985 | MS.gene95358:CDS |
| TTAAGGAGGAAGAGGAAGAG+AGG | 0.509620 | 1.3:-18340306 | MS.gene95358:CDS |
| GTTGCTGGACCATGTACATC+AGG | 0.510248 | 1.3:-18333877 | MS.gene95358:intron |
| TTGAACATAGGTTGATTGAC+TGG | 0.510746 | 1.3:-18333732 | MS.gene95358:CDS |
| GCGGAAGATTGTGGTGGTGG+TGG | 0.517543 | 1.3:+18340537 | None:intergenic |
| TTCATGATATCTGGAATGAA+GGG | 0.518749 | 1.3:-18334289 | MS.gene95358:CDS |
| CTACAATTTAGCACGAGCCC+TGG | 0.523044 | 1.3:+18337002 | None:intergenic |
| GGTAAATGGATCTTCTTCCC+AGG | 0.528565 | 1.3:+18333950 | None:intergenic |
| TGTAAAACTGATCAAGCGAA+TGG | 0.536305 | 1.3:-18334565 | MS.gene95358:CDS |
| GAGGAGGAAGATGAAGATGT+CGG | 0.538123 | 1.3:-18340220 | MS.gene95358:CDS |
| AAGATCTTCCTGGATTTGAA+AGG | 0.540512 | 1.3:-18334443 | MS.gene95358:intron |
| AGGCCAACTCAAGATTCTTG+TGG | 0.543483 | 1.3:-18333653 | MS.gene95358:CDS |
| TCCCCACAAGAATCTTGAGT+TGG | 0.544132 | 1.3:+18333650 | None:intergenic |
| GTAAAACTGATCAAGCGAAT+GGG | 0.549419 | 1.3:-18334564 | MS.gene95358:CDS |
| AGATGTCGGTGTTGTGAAAG+AGG | 0.552906 | 1.3:-18340206 | MS.gene95358:CDS |
| GAAAACAAAGGCGCTTGAGA+AGG | 0.554111 | 1.3:-18340284 | MS.gene95358:CDS |
| AGGCTCGTGTTGAAAGTGCT+TGG | 0.555577 | 1.3:-18336956 | MS.gene95358:CDS |
| TGTGTTCGGCGATGAGCCGA+AGG | 0.559448 | 1.3:-18340341 | MS.gene95358:CDS |
| ATCTTCTCTCACTAATTACA+TGG | 0.562567 | 1.3:-18340131 | MS.gene95358:intron |
| CGGACGGATAAACTTGTGTT+CGG | 0.565605 | 1.3:-18340355 | MS.gene95358:CDS |
| CGAACACAAGTTTATCCGTC+CGG | 0.566071 | 1.3:+18340356 | None:intergenic |
| AAGATTCTTGTGGGGAAGCG+TGG | 0.571176 | 1.3:-18333643 | MS.gene95358:CDS |
| GTTGAGTAAACCCGAGGAGG+AGG | 0.571215 | 1.3:-18340245 | MS.gene95358:CDS |
| GGAATAAGAAATACCGAGAC+CGG | 0.573614 | 1.3:-18340375 | MS.gene95358:CDS |
| AGCGCGGAAGATTGTGGTGG+TGG | 0.574708 | 1.3:+18340534 | None:intergenic |
| GAAGAAAGCGCGGAAGATTG+TGG | 0.575424 | 1.3:+18340528 | None:intergenic |
| ACCACAACTCATGAAGTTGT+GGG | 0.577761 | 1.3:-18338036 | MS.gene95358:CDS |
| ATCATCATAGGTTGGAACAA+AGG | 0.577776 | 1.3:-18338085 | MS.gene95358:intron |
| GACTGGAAAGAGTTGCGAGA+TGG | 0.578343 | 1.3:-18333715 | MS.gene95358:CDS |
| TAGAAGCTAATGAAGAGCTA+AGG | 0.579922 | 1.3:-18333209 | MS.gene95358:CDS |
| GGATCGGGTTCCGGTGTGAG+GGG | 0.585238 | 1.3:+18340496 | None:intergenic |
| CAACAATTACCTGATGTACA+TGG | 0.586750 | 1.3:+18333868 | None:intergenic |
| TTCCATACGATATTGAACAT+AGG | 0.595384 | 1.3:-18333744 | MS.gene95358:CDS |
| GCCCACAACTTCATGAGTTG+TGG | 0.595586 | 1.3:+18338035 | None:intergenic |
| GGGGAGAATGAAGTTTAAGG+AGG | 0.599933 | 1.3:-18340320 | MS.gene95358:CDS |
| GTTGTGGGCGTGTTGACTAA+AGG | 0.601040 | 1.3:-18338021 | MS.gene95358:CDS |
| TGATCAAGCGAATGGGAGAA+CGG | 0.601143 | 1.3:-18334557 | MS.gene95358:CDS |
| ATAGGCAGTAGAACGGCCCT+GGG | 0.601733 | 1.3:-18333967 | MS.gene95358:intron |
| GTGTTCGGCGATGAGCCGAA+GGG | 0.604636 | 1.3:-18340340 | MS.gene95358:CDS |
| TAAGAAATACCGAGACCGGA+CGG | 0.606280 | 1.3:-18340371 | MS.gene95358:CDS |
| CTCCGCCGAACCCCTCACAC+CGG | 0.606587 | 1.3:-18340506 | MS.gene95358:CDS |
| CAACGACGACGAAAAGCCGA+AGG | 0.612882 | 1.3:-18340404 | MS.gene95358:CDS |
| GTAAATGGATCTTCTTCCCA+GGG | 0.615060 | 1.3:+18333951 | None:intergenic |
| TTCCGGTGTGAGGGGTTCGG+CGG | 0.620498 | 1.3:+18340504 | None:intergenic |
| TTATGCAATAGGCAGTAGAA+CGG | 0.620601 | 1.3:-18333974 | MS.gene95358:intron |
| GAGCCTTGGCATCCTTATGA+GGG | 0.621409 | 1.3:+18336973 | None:intergenic |
| ATTCCCTCATAAGGATGCCA+AGG | 0.624838 | 1.3:-18336976 | MS.gene95358:CDS |
| AGAGGAAGAGAGGAAAACAA+AGG | 0.625935 | 1.3:-18340296 | MS.gene95358:CDS |
| ATGTCCATAGACATATCACC+AGG | 0.629127 | 1.3:-18336893 | MS.gene95358:intron |
| ATAGGTTGGAACAAAGGTGG+CGG | 0.633224 | 1.3:-18338079 | MS.gene95358:intron |
| TCATGATATCTGGAATGAAG+GGG | 0.634102 | 1.3:-18334288 | MS.gene95358:CDS |
| ACGAAAAGCCGAAGGTGACG+TGG | 0.639350 | 1.3:-18340396 | MS.gene95358:CDS |
| ATCATAGGTTGGAACAAAGG+TGG | 0.643299 | 1.3:-18338082 | MS.gene95358:intron |
| TGTTCGGCGATGAGCCGAAG+GGG | 0.649435 | 1.3:-18340339 | MS.gene95358:CDS |
| TGCGTTGAGTAAACCCGAGG+AGG | 0.651939 | 1.3:-18340248 | MS.gene95358:CDS |
| CATGATATCTGGAATGAAGG+GGG | 0.674998 | 1.3:-18334287 | MS.gene95358:CDS |
| TTCGTAGTGAGAACTGTCGA+AGG | 0.676280 | 1.3:+18340450 | None:intergenic |
| GCCAACTCAAGATTCTTGTG+GGG | 0.679081 | 1.3:-18333651 | MS.gene95358:CDS |
| AGCTGCGTTGAGTAAACCCG+AGG | 0.680436 | 1.3:-18340251 | MS.gene95358:CDS |
| GGGCTCGTGCTAAATTGTAG+CGG | 0.686569 | 1.3:-18336999 | MS.gene95358:CDS |
| TGTCGGTGTTGTGAAAGAGG+AGG | 0.689428 | 1.3:-18340203 | MS.gene95358:CDS |
| AGATCCATTTACCATGACCG+AGG | 0.703637 | 1.3:-18333940 | MS.gene95358:CDS |
| GAAAGCGCGGAAGATTGTGG+TGG | 0.712487 | 1.3:+18340531 | None:intergenic |
| GTTCGGCGGAGAAGAAAGCG+CGG | 0.728248 | 1.3:+18340518 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 18333158 | 18340614 | 18333158 | ID=MS.gene95358 |
| chr1.3 | mRNA | 18333158 | 18340614 | 18333158 | ID=MS.gene95358.t1;Parent=MS.gene95358 |
| chr1.3 | exon | 18340132 | 18340614 | 18340132 | ID=MS.gene95358.t1.exon1;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18340132 | 18340614 | 18340132 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18338008 | 18338097 | 18338008 | ID=MS.gene95358.t1.exon2;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18338008 | 18338097 | 18338008 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18336894 | 18337039 | 18336894 | ID=MS.gene95358.t1.exon3;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18336894 | 18337039 | 18336894 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18334444 | 18334602 | 18334444 | ID=MS.gene95358.t1.exon4;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18334444 | 18334602 | 18334444 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18334249 | 18334315 | 18334249 | ID=MS.gene95358.t1.exon5;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18334249 | 18334315 | 18334249 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18333878 | 18333985 | 18333878 | ID=MS.gene95358.t1.exon6;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18333878 | 18333985 | 18333878 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18333631 | 18333770 | 18333631 | ID=MS.gene95358.t1.exon7;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18333631 | 18333770 | 18333631 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| chr1.3 | exon | 18333158 | 18333242 | 18333158 | ID=MS.gene95358.t1.exon8;Parent=MS.gene95358.t1 |
| chr1.3 | CDS | 18333158 | 18333242 | 18333158 | ID=cds.MS.gene95358.t1;Parent=MS.gene95358.t1 |
| Gene Sequence |
| Protein sequence |
