AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95829

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95829.t1 MTR_6g053740 95.820 957 38 1 1 955 1 957 0.0 1843
MS.gene95829.t1 MTR_5g053260 33.163 392 254 4 425 813 154 540 1.43e-59 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95829.t1 AT5G23890 47.059 986 443 25 1 949 1 944 0.0 746
MS.gene95829.t1 AT5G23890 48.075 857 376 20 125 949 1 820 0.0 686
MS.gene95829.t1 AT5G52410 57.459 543 200 5 410 928 224 759 0.0 598
MS.gene95829.t1 AT5G52410 35.772 123 59 5 1 117 1 109 2.91e-11 68.2
MS.gene95829.t1 AT5G52410 57.459 543 200 5 410 928 215 750 0.0 598
MS.gene95829.t1 AT5G52410 35.772 123 59 5 1 117 1 109 2.72e-11 68.2
MS.gene95829.t1 AT5G52410 58.317 511 185 4 442 928 2 508 0.0 576
MS.gene95829.t1 AT3G25680 35.219 389 244 3 427 813 171 553 8.67e-63 223

Find 212 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTTCGCTTAAAATCTT+AGG 0.129669 6.2:+44146738 None:intergenic
TATTGGCTTACCATCATAAT+TGG 0.135156 6.2:+44146914 None:intergenic
CAGATGCTGCACCGACTTTC+TGG 0.137059 6.2:-44143822 MS.gene95829:CDS
AAGATTCCTTCTTCTTCTTC+TGG 0.196834 6.2:+44148849 None:intergenic
ATTTCTGAATCTCCAAATTC+TGG 0.205362 6.2:-44146558 MS.gene95829:CDS
CTAGTAGCTGATCTTTCTTC+TGG 0.206191 6.2:-44143756 MS.gene95829:CDS
AAATTACAGGCTGAAGAATT+TGG 0.211510 6.2:-44141576 MS.gene95829:CDS
AGTAGTTTCTGCAGCTTTAC+AGG 0.219788 6.2:-44146499 MS.gene95829:CDS
CATCCTGCTGCTCATTATTA+AGG 0.228977 6.2:+44146889 None:intergenic
TGACAGGGCTGAAAGCTTAT+TGG 0.253989 6.2:-44141715 MS.gene95829:CDS
TCTTCTGATGCCGATATTAC+AGG 0.260713 6.2:-44147335 MS.gene95829:CDS
AGAATGCTGATTTACTTAAA+TGG 0.263355 6.2:+44146646 None:intergenic
GAGAAAAGGGATCCAGAATT+TGG 0.266196 6.2:+44146546 None:intergenic
TCACCAAGAACATACCTTTC+AGG 0.266855 6.2:+44144228 None:intergenic
TGAACTTATGAGCGAACTTT+TGG 0.268578 6.2:+44141421 None:intergenic
AAGCATAGTGCCGTCGAATT+TGG 0.269636 6.2:-44141510 MS.gene95829:CDS
TTTCTGGTTCAGCATTAAAT+TGG 0.274007 6.2:+44146943 None:intergenic
CTTTCAGGGGAGAAGTAAAA+AGG 0.280105 6.2:+44144243 None:intergenic
ACAAGATCCTGGCGCGATAA+AGG 0.293222 6.2:+44143990 None:intergenic
TTTCAGTTTGAAGTGGTATT+TGG 0.300010 6.2:+44147045 None:intergenic
GGTTGGTCCGATTCAGCAAC+TGG 0.306448 6.2:-44148890 MS.gene95829:CDS
ATGGGTCGGGTCTGATTCTA+AGG 0.309439 6.2:-44148927 MS.gene95829:CDS
TGCTCATTATTAAGGTCTAT+TGG 0.310014 6.2:+44146897 None:intergenic
ATAGTCACCTTGAATGTTAT+CGG 0.317196 6.2:+44147223 None:intergenic
TCCAAGAGATAAGGCAAAAT+AGG 0.322176 6.2:+44147543 None:intergenic
GGATCTTGTCAGCTGGAAAA+TGG 0.323426 6.2:-44143976 MS.gene95829:CDS
GGAGCAAGAAAATGGTAAAT+TGG 0.328498 6.2:-44147288 MS.gene95829:CDS
GCAACCTTTCTGTCAGCTTC+AGG 0.332101 6.2:+44143933 None:intergenic
GGAATAATTGCTCTTGCATT+TGG 0.338900 6.2:-44143726 MS.gene95829:intron
TGCTTGTTCTCTCACTCGTT+TGG 0.340913 6.2:+44141875 None:intergenic
TCCAAAACCTCATCTGGTTT+AGG 0.343552 6.2:-44146828 MS.gene95829:CDS
CCTCTAGATCATACTGTAAA+CGG 0.344200 6.2:+44143063 None:intergenic
ACTATTGATGATTCTGATAT+AGG 0.344484 6.2:-44147197 MS.gene95829:CDS
GATATTTCCAAAACCTCATC+TGG 0.348534 6.2:-44146834 MS.gene95829:CDS
GGCCTCCAAAACAAAATTAC+AGG 0.351209 6.2:-44141589 MS.gene95829:CDS
GTTGTGGGAGTAGGAGTTGC+TGG 0.351961 6.2:-44147589 MS.gene95829:CDS
ATTTACTTGTCCTGTAATAT+CGG 0.352628 6.2:+44147325 None:intergenic
GCAGGAATCAAAACCTTTCC+TGG 0.353974 6.2:+44146471 None:intergenic
TTCTAAGGGTGATGGGTTAT+CGG 0.356171 6.2:-44148912 MS.gene95829:CDS
AACAAGTTGGAGCAAGAAAA+TGG 0.356887 6.2:-44147296 MS.gene95829:CDS
CTGATCATCTAATGATGAAT+TGG 0.359092 6.2:+44146614 None:intergenic
CCAATTTATATCAAAATTGA+AGG 0.360066 6.2:-44141616 MS.gene95829:CDS
CAAAAGCTCCTGTTCATAAC+TGG 0.363297 6.2:+44143358 None:intergenic
TTACTTAAATGGTCATTTCC+AGG 0.366462 6.2:+44146657 None:intergenic
ACCTAAACCAGATGAGGTTT+TGG 0.372911 6.2:+44146827 None:intergenic
GAAGGTACAAAGCGAGTTGC+TGG 0.388206 6.2:-44141474 MS.gene95829:CDS
ATTTCTAATCTCACTTGAAC+TGG 0.390712 6.2:+44146860 None:intergenic
TTTCCAGGCTTGGCAGAAGC+TGG 0.396329 6.2:-44144327 MS.gene95829:intron
AGCAAGGGCAATAGCGGCTT+GGG 0.396547 6.2:+44143502 None:intergenic
TTATCTCTTGGAAAACGAAA+TGG 0.397448 6.2:-44147532 MS.gene95829:CDS
GCCGCTATTGCCCTTGCTAC+TGG 0.401253 6.2:-44143497 MS.gene95829:CDS
AAGAGAGGATCAAGAAGCTT+CGG 0.401995 6.2:-44143118 MS.gene95829:CDS
CAATTCATCATTAGATGATC+AGG 0.410592 6.2:-44146613 MS.gene95829:CDS
CTGCTAGCGGTGCTCTTTCA+AGG 0.412353 6.2:-44144647 MS.gene95829:intron
AAAGTTAAGGAATGAGGTTC+AGG 0.412432 6.2:-44143189 MS.gene95829:CDS
TAGTAGCTGATCTTTCTTCT+GGG 0.414299 6.2:-44143755 MS.gene95829:CDS
TGATCTTTCTTCTGGGGAGC+AGG 0.415092 6.2:-44143748 MS.gene95829:CDS
CAACTCAAGGGTCAGTTGAC+AGG 0.415518 6.2:-44141731 MS.gene95829:CDS
AGAACAAGCAAGAGCATTAG+AGG 0.416242 6.2:-44141862 MS.gene95829:CDS
GAAAGATCAGCTACTAGTGC+AGG 0.416302 6.2:+44143762 None:intergenic
GAAGATGATCAAGACAATAT+TGG 0.419569 6.2:-44146723 MS.gene95829:CDS
AGATTCCTTCTTCTTCTTCT+GGG 0.423936 6.2:+44148850 None:intergenic
ACGCCAAGGCCGGCTCTGAA+TGG 0.424465 6.2:-44148946 MS.gene95829:CDS
CCTGAGTCCGATAACATTCA+AGG 0.424789 6.2:-44147230 MS.gene95829:CDS
TATATCAAAATTGAAGGAAT+GGG 0.425466 6.2:-44141610 MS.gene95829:CDS
CAGTGACCCAGTTGCTGAAT+CGG 0.427895 6.2:+44148883 None:intergenic
CATCTTCGCTTAAAATCTTA+GGG 0.430588 6.2:+44146739 None:intergenic
AGAAGAAGACAGTGTTGCCT+TGG 0.434500 6.2:-44143255 MS.gene95829:CDS
GACCCATTCAGAGCCGGCCT+TGG 0.436320 6.2:+44148943 None:intergenic
AGAGGAGGCTAGAGATCGTT+GGG 0.438647 6.2:-44141844 MS.gene95829:CDS
ACTAGTGCAGGGCATGCATT+AGG 0.440026 6.2:+44143774 None:intergenic
GAAGTTACATGGCTCAATGC+TGG 0.440097 6.2:-44141765 MS.gene95829:CDS
CAAGATCCTGGCGCGATAAA+GGG 0.441304 6.2:+44143991 None:intergenic
TGGTGGGGTTGAAGGCTAAT+CGG 0.442642 6.2:+44149047 None:intergenic
CGCCAAGGCCGGCTCTGAAT+GGG 0.442924 6.2:-44148945 MS.gene95829:CDS
CTTTCAGGAGTTGTGGGAGT+AGG 0.444823 6.2:-44147598 MS.gene95829:intron
TGTCAAGTTCTGACCTTGAA+TGG 0.445130 6.2:+44144414 None:intergenic
CGATTCAGCAACTGGGTCAC+TGG 0.449043 6.2:-44148882 MS.gene95829:CDS
TGGCCTTAACAAAACTCAAC+AGG 0.451309 6.2:-44146703 MS.gene95829:CDS
CTGCAGCCCTTTATCGCGCC+AGG 0.451648 6.2:-44143997 MS.gene95829:intron
TCTAAGGGTGATGGGTTATC+GGG 0.452141 6.2:-44148911 MS.gene95829:CDS
AGCGAGTTGCTGGTGATTGT+AGG 0.453284 6.2:-44141464 MS.gene95829:CDS
CTCTAGATCATACTGTAAAC+GGG 0.455789 6.2:+44143064 None:intergenic
TGCGGCTATTGAATCAGAAA+TGG 0.457851 6.2:-44143222 MS.gene95829:CDS
TAGCAAGGGCAATAGCGGCT+TGG 0.459860 6.2:+44143501 None:intergenic
AAATCACCAGGTTGAACATC+AGG 0.461055 6.2:+44144705 None:intergenic
TATTAATGCCAGTTATGAAC+AGG 0.463586 6.2:-44143366 MS.gene95829:CDS
GAATGCCAGGAGCAGAGAAA+AGG 0.464135 6.2:+44146532 None:intergenic
CACCAAGAACATACCTTTCA+GGG 0.466229 6.2:+44144229 None:intergenic
AGAGAGGATCAAGAAGCTTC+GGG 0.466699 6.2:-44143117 MS.gene95829:CDS
TTATATCAAAATTGAAGGAA+TGG 0.469067 6.2:-44141611 MS.gene95829:CDS
TTGTCACAGGTTTATCTGGT+TGG 0.469952 6.2:+44143528 None:intergenic
AGGCCGGCTCTGAATGGGTC+GGG 0.471957 6.2:-44148940 MS.gene95829:CDS
TGACTTGCCTGCCTTTGAGA+TGG 0.477195 6.2:+44141545 None:intergenic
GAAATTGTGTTGTTTGACTC+AGG 0.477685 6.2:+44146765 None:intergenic
ATTGGCTTACCATCATAATT+GGG 0.480181 6.2:+44146915 None:intergenic
CGCGCCAGGATCTTGTCAGC+TGG 0.483759 6.2:-44143983 MS.gene95829:CDS
TAGTTTGCTTTCAGGAGTTG+TGG 0.483993 6.2:-44147605 MS.gene95829:intron
AGTGGCATCATTGATACCAT+TGG 0.492279 6.2:+44147142 None:intergenic
AAGTTACATGGCTCAATGCT+GGG 0.495523 6.2:-44141764 MS.gene95829:CDS
ACTGATGGAGCAGGAATGCC+AGG 0.497805 6.2:+44146519 None:intergenic
GGGTCTGATTCTAAGGGTGA+TGG 0.500951 6.2:-44148920 MS.gene95829:CDS
AACTCAACAGGTCTTATATG+AGG 0.501563 6.2:-44146691 MS.gene95829:CDS
AATGCCAGGAGCAGAGAAAA+GGG 0.505227 6.2:+44146533 None:intergenic
GAAGCCTTTGACAACACATC+AGG 0.506026 6.2:-44147369 MS.gene95829:CDS
TACTTAAATGGTCATTTCCA+GGG 0.506950 6.2:+44146658 None:intergenic
AGACCTTAATAATGAGCAGC+AGG 0.509461 6.2:-44146892 MS.gene95829:CDS
TAGAGGAGGCTAGAGATCGT+TGG 0.514166 6.2:-44141845 MS.gene95829:CDS
TATGAGGAACAGAGTATCCC+TGG 0.516671 6.2:-44146675 MS.gene95829:CDS
AGTTTGCTTTCAGGAGTTGT+GGG 0.517953 6.2:-44147604 MS.gene95829:intron
TGGAATCAGGACTTGGGCAG+AGG 0.518677 6.2:-44141904 MS.gene95829:intron
TGAACCTGATCAAAAGCTGC+AGG 0.521188 6.2:+44146453 None:intergenic
AGGCTTCAGCCGAAGTTACA+TGG 0.522394 6.2:-44141776 MS.gene95829:CDS
TACAGTTGATCCAAATTCGA+CGG 0.527068 6.2:+44141500 None:intergenic
GATCTTTCTTCTGGGGAGCA+GGG 0.527714 6.2:-44143747 MS.gene95829:CDS
TCCGTGCATAACGCCAAGGC+CGG 0.528113 6.2:-44148956 MS.gene95829:CDS
TTGGAGAAGCTCAAACGAAT+GGG 0.528805 6.2:-44141696 MS.gene95829:CDS
GCGAGTTGCTGGTGATTGTA+GGG 0.532203 6.2:-44141463 MS.gene95829:CDS
TTTACAGGTGCCTCCAGGAA+AGG 0.532486 6.2:-44146484 MS.gene95829:CDS
TTTGGATCAACTGTAAAAGA+AGG 0.533995 6.2:-44141492 MS.gene95829:CDS
AGTCTGACGTGATGAGAAAG+AGG 0.539581 6.2:+44149019 None:intergenic
AACTCAAGGGTCAGTTGACA+GGG 0.540042 6.2:-44141730 MS.gene95829:CDS
GCAGCTTTACAGGTGCCTCC+AGG 0.540450 6.2:-44146489 MS.gene95829:CDS
CGGCACTATGCTTTAACTCG+TGG 0.542088 6.2:+44141520 None:intergenic
AAGCTGGACTTATTGAAAGC+AGG 0.543112 6.2:-44144311 MS.gene95829:CDS
GGCACTATGCTTTAACTCGT+GGG 0.544631 6.2:+44141521 None:intergenic
CGCTGGTTGGTATCTGCTAG+CGG 0.545213 6.2:-44144660 MS.gene95829:CDS
GCCGGCCTTGGCGTTATGCA+CGG 0.546961 6.2:+44148955 None:intergenic
TGAAGAGGCAAAACATGAGT+TGG 0.547136 6.2:-44143297 MS.gene95829:CDS
CTTGTTGCATGAGTCAGCTC+GGG 0.547834 6.2:-44147096 MS.gene95829:CDS
GAAAAGCCTTGTCCATGGCC+AGG 0.548184 6.2:-44143034 MS.gene95829:intron
GAAAACATACCCAATTATGA+TGG 0.549370 6.2:-44146924 MS.gene95829:CDS
GCATTATCTATGTACATGGC+AGG 0.549375 6.2:+44144487 None:intergenic
TAAGGCAAAATAGGTAAGCC+CGG 0.551085 6.2:+44147552 None:intergenic
CCGTTTACAGTATGATCTAG+AGG 0.551417 6.2:-44143063 MS.gene95829:CDS
GCTTCCTGAAGCTGACAGAA+AGG 0.553773 6.2:-44143937 MS.gene95829:intron
GGGGAGCAGTACTCAACTCA+AGG 0.554003 6.2:-44141744 MS.gene95829:CDS
ACCTCCTGATGTGTTGTCAA+AGG 0.557320 6.2:+44147365 None:intergenic
CAAGAAAATGGTAAATTGGA+AGG 0.558455 6.2:-44147284 MS.gene95829:CDS
TCTAATGATGAATTGGTCAA+TGG 0.560047 6.2:+44146621 None:intergenic
GAAGCTGCCATCTCAAAGGC+AGG 0.560559 6.2:-44141552 MS.gene95829:CDS
ACTTGTTGCATGAGTCAGCT+CGG 0.560612 6.2:-44147097 MS.gene95829:CDS
AAGGCCGGCTCTGAATGGGT+CGG 0.563902 6.2:-44148941 MS.gene95829:CDS
ACTCAGGAGATGAATAATCC+CGG 0.564022 6.2:+44147246 None:intergenic
AGACCCGACCCATTCAGAGC+CGG 0.565507 6.2:+44148937 None:intergenic
AGCATTGAGCCATGTAACTT+CGG 0.566469 6.2:+44141767 None:intergenic
ATTGGAGAAGCTCAAACGAA+TGG 0.567266 6.2:-44141697 MS.gene95829:CDS
TTCTGATATAGGATCGGAGT+TGG 0.568382 6.2:-44147186 MS.gene95829:CDS
GGCATCATTGATACCATTGG+AGG 0.569021 6.2:+44147145 None:intergenic
CGACGTGTGCATAAATCACC+AGG 0.569114 6.2:+44144693 None:intergenic
GCAGAAACTACTGATGGAGC+AGG 0.569460 6.2:+44146510 None:intergenic
TCGTGAATATGCTCGCTGGT+TGG 0.572821 6.2:-44144673 MS.gene95829:CDS
CCTTGAATGTTATCGGACTC+AGG 0.575875 6.2:+44147230 None:intergenic
AAGGTCAGATAGATACATCC+CGG 0.576945 6.2:-44147265 MS.gene95829:CDS
CAGAAGCATCTCCAGTAGCA+AGG 0.578725 6.2:+44143486 None:intergenic
AATCGGAGTTGAAGAGATGT+TGG 0.578908 6.2:+44149064 None:intergenic
CTCAGGAGATGAATAATCCC+GGG 0.581010 6.2:+44147247 None:intergenic
CAAAGAAGTGGTGGAAGACG+AGG 0.581587 6.2:+44148980 None:intergenic
AGCCGCACGTTCTTTCTCCA+AGG 0.583452 6.2:+44143238 None:intergenic
AGTAAATGCTGAGAACAAGT+TGG 0.583946 6.2:-44147309 MS.gene95829:CDS
TCCAGTAGCAAGGGCAATAG+CGG 0.586351 6.2:+44143496 None:intergenic
AGGGTGATGGGTTATCGGGT+TGG 0.586727 6.2:-44148907 MS.gene95829:CDS
TGATTCCCAGAAGAAGAAGA+AGG 0.587923 6.2:-44148855 MS.gene95829:CDS
AGTAGCTGATCTTTCTTCTG+GGG 0.590948 6.2:-44143754 MS.gene95829:CDS
TTGAACAGATATATATTCAG+TGG 0.591238 6.2:+44147124 None:intergenic
GTTGCTGGTGATTGTAGGGA+AGG 0.591411 6.2:-44141459 MS.gene95829:CDS
TTGCTCCGTGCATAACGCCA+AGG 0.591537 6.2:-44148960 MS.gene95829:CDS
GGTCTGATTCTAAGGGTGAT+GGG 0.592109 6.2:-44148919 MS.gene95829:CDS
ATCAGCACCTAAACCAGATG+AGG 0.597185 6.2:+44146821 None:intergenic
GTCTGACGTGATGAGAAAGA+GGG 0.599108 6.2:+44149020 None:intergenic
AGTAGCATTATCTATGTACA+TGG 0.600902 6.2:+44144483 None:intergenic
AGAAGCATCTCCAGTAGCAA+GGG 0.602025 6.2:+44143487 None:intergenic
TGTCTATAAAACCAGAAAGT+CGG 0.602692 6.2:+44143811 None:intergenic
CGGCTGAAGCCTCCTTGCGG+AGG 0.602998 6.2:+44141787 None:intergenic
TGAACGAAAAGCCTTGTCCA+TGG 0.606463 6.2:-44143039 MS.gene95829:CDS
GTTGGTCCGATTCAGCAACT+GGG 0.606734 6.2:-44148889 MS.gene95829:CDS
TGGAGAAGCTGCCATCTCAA+AGG 0.608787 6.2:-44141556 MS.gene95829:CDS
TGCACGGAGCAAAGAAGTGG+TGG 0.608863 6.2:+44148971 None:intergenic
TGATGATTCTGATATAGGAT+CGG 0.610207 6.2:-44147192 MS.gene95829:CDS
ATTGTTTGGAATCAGGACTT+GGG 0.610422 6.2:-44141910 MS.gene95829:intron
AAGAGATGTTGGTGAACATG+TGG 0.611282 6.2:+44149075 None:intergenic
GCCTTTGACAACACATCAGG+AGG 0.619924 6.2:-44147366 MS.gene95829:CDS
AAAGCTGCAGAAACTACTGA+TGG 0.625334 6.2:+44146504 None:intergenic
GGCTATTGAATCAGAAATGG+AGG 0.628296 6.2:-44143219 MS.gene95829:CDS
AGTTACATGGCTCAATGCTG+GGG 0.628725 6.2:-44141763 MS.gene95829:CDS
AGAGATGTTGGTGAACATGT+GGG 0.631272 6.2:+44149076 None:intergenic
AGGTCAGATAGATACATCCC+GGG 0.632359 6.2:-44147264 MS.gene95829:CDS
CACGTCGTGAATATGCTCGC+TGG 0.643178 6.2:-44144677 MS.gene95829:CDS
ATTGAGCCTGATGTTCAACC+TGG 0.648300 6.2:-44144711 MS.gene95829:CDS
AAAGATCAGCTACTAGTGCA+GGG 0.652793 6.2:+44143763 None:intergenic
TGGGTCGGGTCTGATTCTAA+GGG 0.653419 6.2:-44148926 MS.gene95829:CDS
TTATGCACGGAGCAAAGAAG+TGG 0.654806 6.2:+44148968 None:intergenic
CTTCGGCTGAAGCCTCCTTG+CGG 0.664983 6.2:+44141784 None:intergenic
ACCAAGAACATACCTTTCAG+GGG 0.665184 6.2:+44144230 None:intergenic
TCTGACGTGATGAGAAAGAG+GGG 0.665582 6.2:+44149021 None:intergenic
GACTGAAACTGTTAGTACTG+TGG 0.666312 6.2:-44146793 MS.gene95829:CDS
TGCCTTGGAGAAAGAACGTG+CGG 0.673296 6.2:-44143240 MS.gene95829:CDS
GGAGAGACACGGAATCAAAG+TGG 0.674644 6.2:-44141823 MS.gene95829:CDS
ACAAGCAAGAGCATTAGAGG+AGG 0.676263 6.2:-44141859 MS.gene95829:CDS
AGAGATCGTTGGGAGAGACA+CGG 0.678075 6.2:-44141834 MS.gene95829:CDS
GAAGTGGTGGAAGACGAGGT+TGG 0.681051 6.2:+44148984 None:intergenic
GGGAGCAGTACTCAACTCAA+GGG 0.682992 6.2:-44141743 MS.gene95829:CDS
ATATAAATTGGGAAACCACG+AGG 0.685060 6.2:+44141628 None:intergenic
ACAAAGTCTAATGAGTAACA+AGG 0.685125 6.2:-44143159 MS.gene95829:CDS
GAGACACGGAATCAAAGTGG+TGG 0.696553 6.2:-44141820 MS.gene95829:CDS
AATTGACAAGATCATCCTCG+TGG 0.704462 6.2:-44141643 MS.gene95829:CDS
GGTTGACGAAGACCTCCGCA+AGG 0.709010 6.2:-44141799 MS.gene95829:CDS
CTGACGTGATGAGAAAGAGG+GGG 0.713118 6.2:+44149022 None:intergenic
ATTTGATGACATCACCCCTG+AGG 0.716657 6.2:-44144452 MS.gene95829:CDS
GGAATCAAAACCTTTCCTGG+AGG 0.723513 6.2:+44146474 None:intergenic
TGACGAAGACCTCCGCAAGG+AGG 0.731713 6.2:-44141796 MS.gene95829:CDS
CGAATGGGTGCAGATCTCAG+AGG 0.766959 6.2:-44141681 MS.gene95829:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.2 gene 44141413 44149113 44141413 ID=MS.gene95829
chr6.2 mRNA 44141413 44149113 44141413 ID=MS.gene95829.t1;Parent=MS.gene95829
chr6.2 exon 44148843 44149113 44148843 ID=MS.gene95829.t1.exon1;Parent=MS.gene95829.t1
chr6.2 CDS 44148843 44149113 44148843 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44147527 44147613 44147527 ID=MS.gene95829.t1.exon2;Parent=MS.gene95829.t1
chr6.2 CDS 44147527 44147613 44147527 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44146419 44147407 44146419 ID=MS.gene95829.t1.exon3;Parent=MS.gene95829.t1
chr6.2 CDS 44146419 44147407 44146419 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44144648 44144736 44144648 ID=MS.gene95829.t1.exon4;Parent=MS.gene95829.t1
chr6.2 CDS 44144648 44144736 44144648 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44144428 44144531 44144428 ID=MS.gene95829.t1.exon5;Parent=MS.gene95829.t1
chr6.2 CDS 44144428 44144531 44144428 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44144243 44144342 44144243 ID=MS.gene95829.t1.exon6;Parent=MS.gene95829.t1
chr6.2 CDS 44144243 44144342 44144243 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44143938 44144013 44143938 ID=MS.gene95829.t1.exon7;Parent=MS.gene95829.t1
chr6.2 CDS 44143938 44144013 44143938 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44143727 44143841 44143727 ID=MS.gene95829.t1.exon8;Parent=MS.gene95829.t1
chr6.2 CDS 44143727 44143841 44143727 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44143035 44143566 44143035 ID=MS.gene95829.t1.exon9;Parent=MS.gene95829.t1
chr6.2 CDS 44143035 44143566 44143035 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
chr6.2 exon 44141413 44141917 44141413 ID=MS.gene95829.t1.exon10;Parent=MS.gene95829.t1
chr6.2 CDS 44141413 44141917 44141413 ID=cds.MS.gene95829.t1;Parent=MS.gene95829.t1
Gene Sequence

>MS.gene95829

ATGGCTTCAATTTCACCCACATGTTCACCAACATCTCTTCAACTCCGATTAGCCTTCAACCCCACCAAACCCCCTCTTTCTCATCACGTCAGACTTCGCAACATCAACCAACCTCGTCTTCCACCACTTCTTTGCTCCGTGCATAACGCCAAGGCCGGCTCTGAATGGGTCGGGTCTGATTCTAAGGGTGATGGGTTATCGGGTTGGTCCGATTCAGCAACTGGGTCACTGGATAATGATTCCCAGAAGAAGAAGAAGGAATCTTTTCGAGGTGTGTGTATGGTTCTTGAAATTCTGATTCATTTTGTTTATTTTTTGTTTTTTGTTTTTTAGTGATAAATTTGTGTTTTTTTATTGGAATTTTGTTAATGTGCAATAAAAAGTTGAAATTTTTCTAATTGTTGAGTTGAAGTTAAGTGCTTTGGTTGAATTGTATCATATCATATCATCATTATGATCCATGATACTGGATATGAAACTGACACTAACACGTGTACACAGACACTTCTGATAGAAGTTGTGTCTGTTGTCTGACATGAGAAACACCAACACATGTGATTACGCTCACTTATGTCACTTTTTTAAATTGTTAGTGGTGTTGACGTGTGTTCGTGTTGTGTTTGGTATTTTTGTTACTCTTGTATATTGGTGTTGTGTTGAACACTGACATGTGTCAGACACACCTGCAATATGAAGTGATGGGTCTACATAGGTTATGCTAACAAGTGTATACATTAGATCAGGAATTAAGAAATGAAAAGTATTTATCCAAAGAGACGTTTGATATAGGATTTGTTTCAGACCTGTGCGTATGTAGTGTTTTCAATCGCATATCTCGGAAGAAAGAGGTTTTTTAAACGTTCTGCTATGCTACAGTGCTATAGCGCCACTATAACTGCTATTTGACAATACTTTGTACCAAATAGCTTATTGTGGAACAACGGCGGTTTGGTCAAATTCCGCTACACTATAGTGTTGTATTGCCACTGTTGCTTCTATTTGACAACACTTTGTACTAAATAGATATCACTGAACAATATTGCGTTGTTCAAACTCCGCTACACTACAGTGTTGTATTGCCACTATAGCTACTATTTGACATGTATATGGAAAAACCATATTTTCAAAGAGGTAAATTCTTTAAATGAGTCCTCATAATTTGAGGGATACCATGGGTTAACCAAAATATTAGGCATTTGAGGCCACACAGCATTTGCCTTATTATTGTATTGTCTAATAAATATTTTTTTATTTTTTTTAACTTCTTAAAGGCAATGAGGCCACAATTAGCATTTTCCTTATTATTATTAGTGCTGAGGAAACTTTTGTTTATAATTGAACTTGATTTTAACTTGGTTATTACTATTAGATTCAATATTATCAAATCAGCATGACCTGGCATAGTTGAGGATTTGAGAGAATGGATGAGAAGAGTTTGTGTTTTTTCTGTTAATTGTTTCCACACTAATTAATAACACTTTTGAAGTAGTTTGCTTTCAGGAGTTGTGGGAGTAGGAGTTGCTGGAGTCCTTCTTTTTACCGGGCTTACCTATTTTGCCTTATCTCTTGGAAAACGAAATGGTTCCAGTAAGTAATCCTAGCTGGCTTCTCTCATGCGATTTTTCATCATGTTTTGTCAAATAGGGAGTTTGTTTGTGAGAACTGATTTTTCCATTGCATTTTTACATTTCTGTGATTGGATACAGAAGAGGAGCAACAATTGAAGCCTTTGACAACACATCAGGAGGTGCTTTTGTCTTCTGATGCCGATATTACAGGACAAGTAAATGCTGAGAACAAGTTGGAGCAAGAAAATGGTAAATTGGAAGGTCAGATAGATACATCCCGGGATTATTCATCTCCTGAGTCCGATAACATTCAAGGTGACTATAGAACTATTGATGATTCTGATATAGGATCGGAGTTGGTATACGATAGCAAAAACACCTCCAATGGTATCAATGATGCCACTGAATATATATCTGTTCAAGAAGACTTGTTGCATGAGTCAGCTCGGGATGACGAGTTAATTGTTCCCAGTGAAAACCAAATACCACTTCAAACTGAAAATACTCTTGATTCTTTTAGTGATTTTGATAGCAATTCGGCTGTAGATAAAACAGAATCTACTGCCTATCTTAAAGAAAACCAATTTAATGCTGAACCAGAAAACATACCCAATTATGATGGTAAGCCAATAGACCTTAATAATGAGCAGCAGGATGAAATAACCAGTTCAAGTGAGATTAGAAATTCTGATATTTCCAAAACCTCATCTGGTTTAGGTGCTGATAATAAGACTGAAACTGTTAGTACTGTGGTAAATCCTGAGTCAAACAACACAATTTCTGACCCTAAGATTTTAAGCGAAGATGATCAAGACAATATTGGCCTTAACAAAACTCAACAGGTCTTATATGAGGAACAGAGTATCCCTGGAAATGACCATTTAAGTAAATCAGCATTCTCACCATTGACCAATTCATCATTAGATGATCAGGTTAGAAATGAGAATAATGAGATTAATAAAGAAATTTCTGAATCTCCAAATTCTGGATCCCTTTTCTCTGCTCCTGGCATTCCTGCTCCATCAGTAGTTTCTGCAGCTTTACAGGTGCCTCCAGGAAAGGTTTTGATTCCTGCAGCTTTTGATCAGGTTCAGGGACATGCACTAGCTGCATTGCAAGTTTTGAAGGTAAATACTTCGTCTCATTCACATATTTCAAGTCAAGATGACTATGATGCTGTGTGCATTTACTATTATTTTTAACAGGTTTTTATATCTAGTCCTGTATTTCGAAATTTACACACAATGTATATATAAAATGAGCTGCAAAGTAGTTCTAATCCAAGTTTTCAATAGAACTTAAATAAGACCTCTTAAAATTTGGGTTATGCCAAACTCAACCCTACAAAACAGGCTTGTAAAGGGAAGGGTGCCACTCCTTATAAATTCCTTTCAGGCATTATCTCTTTTCAATGTTAACTTCAATTTTTCCCAATACACCTTCTCACATCCAACACTATTGGGCTGGATGCGTGGATAATATGGCAAGTGGACCGCATCAATGGACTCCGATACCATCTCTGTATTGAGTCTAACTCATCCTTACAAAATCAGCTTTTCAGGTGAATATAGCCACTCTTTATAAACTATTTTCAAACCTAATCTATTTTCAACGTGGTACTTGGATTTTCCCCAATAAGAACAAATGATACCCTCATAGAAACTGCCCAAAAATTTACTAAATATTGTGCTGACTTAAACTTGAGCTATATATTTGGTCCATTGTGTTGTGTTACTAAGCCATGCTCTATCTTATTGGTGATGCTGGAAAGAACAATCTATCTTTCTTTTGGGAAAAAAGACCTTGAAAACAACTGAAATTAATAATTCCATTTAGCTTTATTTTTATTTTTATTGAAGGAATAGTAGGTTTAGGATTATTTTTGTAGAGTAAAATGTGTGCAATTTAATTACTTACCGAAAGATGTGCATGTGTTGAAGTTATGAGTTATAGATAAAAGGAGGAGAAACATAATAAGAGCTTGAATGTTATTGAGTACTCCCTCCGGTCCTTTTATAAGAGACAAAATGAGTTTTTTTTTTTTTTTTTTTGTTGTCCTTTTTATAAGAAACAATCTTCAAATTTAAACTCTATGAATTATCCAATACCATGTATACCCTTATTAAATAAAGTTAATGATAACAACAAATACCAACTTCCAATGCAAGTGCCTTGGATATATACATCTCCTTGCACGTTAATTGGCTTACCGGTGAAGTATTAATCATAGGAGTAATTCAATCCACCATTATATAAAAGATAATTTTTGTTAAAAAAAAAAACTTTTAGAATCATTAATACAAAAAAGTGATTTACCTGGTCCATGTGAATTTCTGGAATTGTCTCTTATAAAAAGGACCGGAGGGAGTATAACTTTGCTAATTGCTTGATACAAACGACTAACCACTAACCACTGTTTGTAGGGATATTCAACTTACAAGAGTCCTAATTCTAAATAAGGAGATTAGAAAACAAATCATAGTGATAAACTAAGAAAGTGTCTCATCTTCAATATTTCCGTCTCAAATTATGTCATTTAACAATACCAAGACAACCTCTATTAACATTTTCCAACAATCCCCTCACTTAATTATAAGTAGTCCGCCTAACTACTACACTGCTACTAATGTCATCCACTCTAATAAATTTTGAGTATTTTAGGCTATAGACACTATTTTTTGGTTGAATTCAACATAGCTAATGATTTTATTGAGAGGTGCGCATTACTTTAAAAAGACACATAATATGAGATGGAGTATTTTTGTTCACTCGGTTGTCATTATTCATTTAAAACCTGTTAATTCCTCAGGTCATTGAGCCTGATGTTCAACCTGGTGATTTATGCACACGTCGTGAATATGCTCGCTGGTTGGTATCTGCTAGCGGTGCTCTTTCAAGGTAGTAGTTTATTTCTTCTTTCTGGATGATATGAATGACAAGATGTATACAACGGGTTACTATTTTCTACACATGTAATATTTGGCATTACACGTGCATTTGTGGTTACTTTTCAGGAATGCGGTTTCGAAAGTGTATCCTGCCATGTACATAGATAATGCTACTGAACTTGCATTTGATGACATCACCCCTGAGGACCCTGATTTTTCTTCCATTCAAGGTCAGAACTTGACATGATTATCATTTTTAGCAATACAACCACTTGGTCTATGCCATTATGTTATCTGAAGAATATTGTTTTCCAGGCTTGGCAGAAGCTGGACTTATTGAAAGCAGGCTCTCAAGACGTGATGCACAGTTGTCTGCTAATGAAGACAATAGCCCTTTTTACTTCTCCCCTGAAAGGTATGTTCTTGGTGAATCTGAATATCTGGTTTGCGGTTACTATTTCCCACCCTCAGTAGCAGTGCAGTCTTCAGAATTTTTTGCAGTTGTACTTTATATTTTTGGAGAAAAAACTACTTTTTTGTTTTTAGCTAGTGCATAGATGTATCTCATTCTGTTTCAGACATGCTTATAACTTTATGACAGACTATAATTATGATCTCCCTATCTCATATGTTAATTTCTGCAGCCCTTTATCGCGCCAGGATCTTGTCAGCTGGAAAATGGCCCTAGAGAAAAGACAGCTTCCTGAAGCTGACAGAAAGGTTGCTCAACTCGCGGTTTAATCTTGTTATATTCTTAAGCCATCTTGATTCCATCTGCATTGAACTGATGAATATATTTATCGAATCTAATGGCAGATGCTGCACCGACTTTCTGGTTTTATAGACACTGATAAGATACATCCTAATGCATGCCCTGCACTAGTAGCTGATCTTTCTTCTGGGGAGCAGGGAATAATTGCTCTTGCATTTGGTAATTCATTCTCTTCGTAAATAATAAATATGTTTAAATGTTTGAGATCTGCTTCATAATAAAAGCTTATTTGATATTCGTTTTCTCCTATACTTGTTTTGAATCTGCTTTTAGTTGACTTGTTCATTTTTCCCCCTGGATGGGGGGCTGTTTATTCAAGGTTATACACGATTGTTCCAACCAGATAAACCTGTGACAAAAGCCCAAGCCGCTATTGCCCTTGCTACTGGAGATGCTTCTGACATAGTTAGTGAAGAGCTTGCACGCATTGAAGCAGAATCTATTGCTGAAAATGCTGTTGCTGCACATAGTGCTTTAGTAGCTCAAGTTGAGAGAGATATTAATGCCAGTTATGAACAGGAGCTTTTGTTAGAGAGGGAAAAGATCAATGCAATTGAAAAAATGGCTGAAGAGGCAAAACATGAGTTGGAAACGTTAAGAGCTCAGAGAGAAGAAGACAGTGTTGCCTTGGAGAAAGAACGTGCGGCTATTGAATCAGAAATGGAGGTTTTTTCAAAGTTAAGGAATGAGGTTCAGGATCAATTACAAAGTCTAATGAGTAACAAGGTAGAAATAGCATATGAAAAAGAGAGGATCAAGAAGCTTCGGGAGCAGACAGAAATTGAAAACAACGAGATTACCCGTTTACAGTATGATCTAGAGGTTGAACGAAAAGCCTTGTCCATGGCCAGGTAAAATCATGAAACTTTTAAAGGCAACTAAAATATGTCTCATCAACTCTCAATTTTGGTTTGATATATCTTGGGTTGGTTCTAACTCAACATAAGTTGAAGACTGCCTCCCACGTATAAACTCATTTTTTGGCCTTATCTCATCCAATGTGAGACTTTTAACACACCTCCCTCATGCCCAAGACTGGACATCTGGAGTTTGACCCGAGAGGCCTGATAGTGTGGCCAATAGATCTTGGATAGGCTTTGATACCATCTTAGAATTTGGGTGAGATGTCTCCCACTTATAGACTCATTTTCAGGCCATATCTAATTCAATGTGGGTCTCTTAACAATATTAATGCTAAAAAAGTGGTATCTAGAATTATTGGCAAATGTCTTTTATTCACCACTTTTTCCTTTGGTTTGGGATTGAATTAAACAAGTATTCCATACCATCCAATCAAGGTGTGAGGGAGGTGATAGTAGTACTAAGGAATATGAACTTCCAATTAGAGAAATAATAAAGTAACACTGAAATCCGAAAGGTTTAATATACGATTTCTCACAAATATCAAGTCAACTAGCGTACAGCTTCCCATGTCTGCTACTATGGTTGTTATTGCGATATGCTTCGGCACATTAGTGCTTGTGTCTCAAAAGAAAATGAGTGAATGAAATATGAAAAACAAAATAAGTGGTAACAATGGTTGTTATTGCGATATGCTTCGGCACATTAGTGCTTGTGTCTCAAAAGAATATGAGTGAATGAAATATGAAAAACAAAATAAGTGGTAACAACTCAAATTTTTTACATCTATACTATGTTAATTAAACAAACAATCACCATGTATGTGTTTATCTCTTGCCTGCATATTTGTTTTGCAGCCACCATATTATATAACAATTGCTTGCAATTGTATTTTCAGTGCACTTTTTTTTCTTGACCTACTTGAATTTGTGCACTGATGTTCGGCACTTCATAGTACTCTGAACAGTTGTTTCTTCACATAGATGAATTAGTTCAGAAAAAATAAGTTTTATGTGTCTTTTATTCTTGAGTCAAAATCATAGCTCTTCTAAGCTTATCAAAAACCAGAATAAATGTTTTAAATATATGTTAATTGTTTGGAATCAGGACTTGGGCAGAGGATGAAGCCAAACGAGTGAGAGAACAAGCAAGAGCATTAGAGGAGGCTAGAGATCGTTGGGAGAGACACGGAATCAAAGTGGTGGTTGACGAAGACCTCCGCAAGGAGGCTTCAGCCGAAGTTACATGGCTCAATGCTGGGGAGCAGTACTCAACTCAAGGGTCAGTTGACAGGGCTGAAAGCTTATTGGAGAAGCTCAAACGAATGGGTGCAGATCTCAGAGGAAAATGCAGAGAAACAATTGACAAGATCATCCTCGTGGTTTCCCAATTTATATCAAAATTGAAGGAATGGGCCTCCAAAACAAAATTACAGGCTGAAGAATTTGGAGAAGCTGCCATCTCAAAGGCAGGCAAGTCAGCCCACGAGTTAAAGCATAGTGCCGTCGAATTTGGATCAACTGTAAAAGAAGGTACAAAGCGAGTTGCTGGTGATTGTAGGGAAGGAGTTGAGAAAATCACCCAAAAGTTCGCTCATAAGTTCAAGACTTGA
Protein sequence

>MS.gene95829.t1

MASISPTCSPTSLQLRLAFNPTKPPLSHHVRLRNINQPRLPPLLCSVHNAKAGSEWVGSDSKGDGLSGWSDSATGSLDNDSQKKKKESFRGVVGVGVAGVLLFTGLTYFALSLGKRNGSKEEQQLKPLTTHQEVLLSSDADITGQVNAENKLEQENGKLEGQIDTSRDYSSPESDNIQGDYRTIDDSDIGSELVYDSKNTSNGINDATEYISVQEDLLHESARDDELIVPSENQIPLQTENTLDSFSDFDSNSAVDKTESTAYLKENQFNAEPENIPNYDGKPIDLNNEQQDEITSSSEIRNSDISKTSSGLGADNKTETVSTVVNPESNNTISDPKILSEDDQDNIGLNKTQQVLYEEQSIPGNDHLSKSAFSPLTNSSLDDQVRNENNEINKEISESPNSGSLFSAPGIPAPSVVSAALQVPPGKVLIPAAFDQVQGHALAALQVLKVIEPDVQPGDLCTRREYARWLVSASGALSRNAVSKVYPAMYIDNATELAFDDITPEDPDFSSIQGLAEAGLIESRLSRRDAQLSANEDNSPFYFSPESPLSRQDLVSWKMALEKRQLPEADRKMLHRLSGFIDTDKIHPNACPALVADLSSGEQGIIALAFGYTRLFQPDKPVTKAQAAIALATGDASDIVSEELARIEAESIAENAVAAHSALVAQVERDINASYEQELLLEREKINAIEKMAEEAKHELETLRAQREEDSVALEKERAAIESEMEVFSKLRNEVQDQLQSLMSNKVEIAYEKERIKKLREQTEIENNEITRLQYDLEVERKALSMARTWAEDEAKRVREQARALEEARDRWERHGIKVVVDEDLRKEASAEVTWLNAGEQYSTQGSVDRAESLLEKLKRMGADLRGKCRETIDKIILVVSQFISKLKEWASKTKLQAEEFGEAAISKAGKSAHELKHSAVEFGSTVKEGTKRVAGDCREGVEKITQKFAHKFKT