AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96093

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96093.t1 MTR_7g075040 95.882 170 7 0 1 170 26 195 3.91e-117 329
MS.gene96093.t1 MTR_8g036075 69.536 151 45 1 18 168 45 194 9.07e-70 210
MS.gene96093.t1 MTR_5g079340 48.322 149 63 5 19 165 1 137 6.44e-35 119
MS.gene96093.t1 MTR_1g032070 44.966 149 73 4 17 165 41 180 2.95e-34 119
MS.gene96093.t1 MTR_1g046950 43.046 151 71 4 19 165 1 140 9.31e-33 114
MS.gene96093.t1 MTR_3g067610 44.444 144 67 4 22 165 5 135 2.99e-32 112
MS.gene96093.t1 MTR_8g105230 44.667 150 74 3 19 168 33 173 3.32e-30 108
MS.gene96093.t1 MTR_7g090450 39.735 151 87 2 15 165 66 212 3.96e-30 109
MS.gene96093.t1 MTR_4g067270 41.558 154 73 4 20 168 3 144 5.75e-30 107
MS.gene96093.t1 MTR_8g107110 44.000 150 72 4 21 169 23 161 9.34e-30 107
MS.gene96093.t1 MTR_1g047100 42.282 149 78 4 19 167 1 141 9.34e-30 106
MS.gene96093.t1 MTR_5g017550 39.157 166 89 4 4 169 32 185 5.29e-27 100
MS.gene96093.t1 MTR_3g089090 38.889 144 78 3 23 166 6 139 6.16e-27 99.4
MS.gene96093.t1 MTR_3g089070 38.889 144 78 3 23 166 7 140 6.47e-27 99.4
MS.gene96093.t1 MTR_7g087610 39.310 145 79 3 21 165 11 146 6.51e-27 99.4
MS.gene96093.t1 MTR_5g088320 39.865 148 80 3 18 165 8 146 7.10e-27 99.4
MS.gene96093.t1 MTR_6g025320 40.000 145 78 3 21 165 11 146 1.06e-26 99.0
MS.gene96093.t1 MTR_6g079570 41.497 147 77 3 21 167 25 162 1.97e-26 99.0
MS.gene96093.t1 MTR_1g076650 39.860 143 77 3 23 165 13 146 3.36e-25 95.1
MS.gene96093.t1 MTR_2g098890 38.125 160 91 4 10 168 53 205 9.56e-25 95.5
MS.gene96093.t1 MTR_7g089760 38.514 148 82 3 22 168 5 144 2.59e-24 92.8
MS.gene96093.t1 MTR_4g086260 39.310 145 77 4 21 165 56 189 3.27e-24 94.0
MS.gene96093.t1 MTR_1g041285 39.726 146 79 3 22 167 12 148 3.91e-24 92.4
MS.gene96093.t1 MTR_5g017510 37.349 166 93 4 4 169 32 186 4.69e-24 93.2
MS.gene96093.t1 MTR_5g017560 37.349 166 93 4 4 169 32 186 4.69e-24 93.2
MS.gene96093.t1 MTR_6g023460 39.333 150 79 4 17 165 3 141 4.73e-24 92.0
MS.gene96093.t1 MTR_4g103650 39.583 144 77 3 22 165 2 135 1.62e-23 90.5
MS.gene96093.t1 MTR_4g103630 38.667 150 82 3 17 166 1 140 1.96e-23 90.1
MS.gene96093.t1 MTR_5g008705 40.146 137 73 3 29 165 1 128 3.45e-23 89.4
MS.gene96093.t1 MTR_4g103655 38.255 149 82 3 17 165 1 139 1.02e-22 88.6
MS.gene96093.t1 MTR_4g115170 40.506 158 81 4 22 168 64 219 2.27e-22 89.7
MS.gene96093.t1 MTR_7g074020 37.671 146 82 3 22 167 30 166 1.11e-20 84.0
MS.gene96093.t1 MTR_8g066630 32.468 154 98 3 15 168 10 157 1.36e-20 83.6
MS.gene96093.t1 MTR_6g007613 37.241 145 82 3 21 165 28 163 3.63e-20 82.8
MS.gene96093.t1 MTR_7g074240 36.601 153 90 4 20 167 22 172 4.84e-20 82.4
MS.gene96093.t1 MTR_1g071150 34.028 144 85 3 22 165 12 145 7.41e-19 78.6
MS.gene96093.t1 MTR_5g008695 39.496 119 65 3 46 164 1 112 2.81e-18 76.3
MS.gene96093.t1 MTR_4g107490 32.099 162 98 4 10 170 366 516 2.90e-18 81.3
MS.gene96093.t1 MTR_0168s0120 38.655 119 64 3 47 165 1 110 4.51e-18 75.5
MS.gene96093.t1 MTR_6g025320 38.655 119 64 3 47 165 1 110 4.51e-18 75.5
MS.gene96093.t1 MTR_8g078270 36.111 144 81 4 23 165 54 187 5.65e-18 77.4
MS.gene96093.t1 MTR_4g112460 30.625 160 101 5 13 170 2 153 2.22e-17 75.5
MS.gene96093.t1 MTR_7g068710 31.325 166 104 3 5 170 344 499 3.65e-17 78.2
MS.gene96093.t1 MTR_5g009830 36.486 148 79 5 23 168 372 506 4.51e-17 77.8
MS.gene96093.t1 MTR_3g083470 34.000 150 80 3 19 168 90 220 1.66e-16 74.3
MS.gene96093.t1 MTR_8g095440 32.877 146 86 3 23 168 336 469 2.30e-16 75.9
MS.gene96093.t1 MTR_7g074020 35.878 131 75 3 21 151 29 150 2.70e-16 72.4
MS.gene96093.t1 MTR_0028s0170 30.822 146 90 3 23 168 384 518 4.47e-16 75.1
MS.gene96093.t1 MTR_1g026190 32.653 147 86 4 23 168 388 522 5.90e-16 74.7
MS.gene96093.t1 MTR_6g007613 34.848 132 77 3 21 152 28 150 6.28e-16 71.2
MS.gene96093.t1 MTR_8g069915 32.026 153 90 6 22 170 12 154 7.58e-16 71.2
MS.gene96093.t1 MTR_4g132070 34.014 147 84 4 23 168 397 531 1.16e-15 73.9
MS.gene96093.t1 MTR_1g096490 33.108 148 87 3 23 170 336 471 1.29e-15 73.6
MS.gene96093.t1 MTR_8g099095 34.459 148 82 5 23 168 372 506 3.71e-15 72.4
MS.gene96093.t1 MTR_5g092810 34.014 147 84 4 23 168 398 532 4.30e-15 72.4
MS.gene96093.t1 MTR_1g030440 35.099 151 83 5 20 165 20 160 5.44e-15 68.9
MS.gene96093.t1 MTR_3g051770 33.775 151 86 4 23 168 389 530 1.33e-14 70.9
MS.gene96093.t1 MTR_3g098090 30.137 146 90 3 23 168 338 471 1.38e-14 70.9
MS.gene96093.t1 MTR_5g089320 30.137 146 91 3 23 168 374 508 1.61e-14 70.5
MS.gene96093.t1 MTR_1g055255 32.192 146 87 3 23 168 430 563 2.02e-14 70.5
MS.gene96093.t1 MTR_3g088655 32.215 149 98 3 20 165 6 154 2.20e-14 67.4
MS.gene96093.t1 MTR_7g115040 35.811 148 86 3 18 165 8 146 2.20e-14 67.0
MS.gene96093.t1 MTR_3g098070 30.137 146 90 3 23 168 335 468 2.65e-14 70.1
MS.gene96093.t1 MTR_1g041150 30.822 146 89 3 23 168 424 557 1.25e-13 68.2
MS.gene96093.t1 MTR_7g106710 31.507 146 88 3 23 168 441 574 1.58e-13 67.8
MS.gene96093.t1 MTR_4g082050 50.746 67 32 1 102 168 76 141 2.46e-13 63.9
MS.gene96093.t1 MTR_1g054865 32.432 148 80 5 23 166 467 598 4.14e-13 66.6
MS.gene96093.t1 MTR_5g011850 31.944 144 83 4 24 165 14 144 1.11e-12 62.4
MS.gene96093.t1 MTR_1g052530 30.435 161 102 3 10 170 345 495 1.63e-12 64.7
MS.gene96093.t1 MTR_7g091890 28.571 161 105 3 10 170 351 501 1.63e-12 64.7
MS.gene96093.t1 MTR_5g027000 31.847 157 93 6 15 167 12 158 2.80e-12 61.6
MS.gene96093.t1 MTR_4g132040 31.973 147 87 4 23 168 401 535 1.09e-11 62.4
MS.gene96093.t1 MTR_4g066660 29.054 148 94 4 23 170 378 514 2.71e-11 61.2
MS.gene96093.t1 MTR_4g122260 34.483 145 72 7 18 152 12 143 8.90e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96093.t1 AT1G24620 45.333 150 67 4 22 168 37 174 4.92e-33 116
MS.gene96093.t1 AT1G18210 41.722 151 75 4 15 165 16 153 1.20e-28 104
MS.gene96093.t1 AT1G18210 41.722 151 75 4 15 165 16 153 1.20e-28 104
MS.gene96093.t1 AT1G66400 41.096 146 73 4 23 165 16 151 2.50e-28 103
MS.gene96093.t1 AT5G37770 43.151 146 78 3 23 168 18 158 7.28e-28 102
MS.gene96093.t1 AT5G37770 43.151 146 78 3 23 168 18 158 7.28e-28 102
MS.gene96093.t1 AT1G05990 43.333 150 77 3 20 168 3 145 1.19e-27 101
MS.gene96093.t1 AT5G37780 39.865 148 80 3 18 165 8 146 2.64e-27 100
MS.gene96093.t1 AT1G66410 39.865 148 80 3 18 165 8 146 2.64e-27 100
MS.gene96093.t1 AT5G21274 40.690 145 77 3 21 165 11 146 5.26e-27 99.8
MS.gene96093.t1 AT3G56800 40.690 145 77 3 21 165 11 146 5.68e-27 99.8
MS.gene96093.t1 AT2G27030 40.690 145 77 3 21 165 11 146 5.68e-27 99.8
MS.gene96093.t1 AT2G41110 40.690 145 77 3 21 165 11 146 5.68e-27 99.8
MS.gene96093.t1 AT2G43290 39.333 150 87 3 20 168 63 209 8.70e-27 100
MS.gene96093.t1 AT3G10190 37.107 159 92 2 7 165 55 205 1.08e-26 100
MS.gene96093.t1 AT3G43810 40.000 145 78 3 21 165 11 146 1.11e-26 99.0
MS.gene96093.t1 AT2G27030 40.690 145 77 3 21 165 11 146 1.25e-26 99.8
MS.gene96093.t1 AT3G59440 40.816 147 77 3 23 168 52 189 1.36e-26 100
MS.gene96093.t1 AT3G43810 40.000 145 78 3 21 165 11 146 8.10e-26 98.6
MS.gene96093.t1 AT1G73630 37.952 166 86 5 4 169 6 154 9.49e-26 97.1
MS.gene96093.t1 AT5G42380 37.671 146 83 3 21 166 48 185 2.06e-25 96.7
MS.gene96093.t1 AT3G22930 40.278 144 77 3 22 165 35 169 3.43e-25 95.9
MS.gene96093.t1 AT3G07490 38.514 148 82 3 22 168 5 144 5.25e-25 94.7
MS.gene96093.t1 AT4G03290 39.073 151 86 3 20 169 3 148 1.67e-24 93.6
MS.gene96093.t1 AT2G41110 37.580 157 77 4 21 165 11 158 5.73e-24 92.4
MS.gene96093.t1 AT5G37780 36.196 163 80 4 18 165 8 161 5.45e-23 89.7
MS.gene96093.t1 AT4G14640 38.621 145 80 3 21 165 12 147 5.79e-23 89.4
MS.gene96093.t1 AT1G32250 30.519 154 104 2 15 168 9 159 5.04e-22 87.4
MS.gene96093.t1 AT2G15680 36.735 147 82 4 21 167 49 184 6.79e-22 87.4
MS.gene96093.t1 AT5G17470 37.324 142 80 4 25 165 5 138 1.73e-21 85.5
MS.gene96093.t1 AT5G37780 33.908 174 80 4 18 165 8 172 2.00e-21 86.3
MS.gene96093.t1 AT1G21550 37.500 152 82 4 20 165 8 152 2.46e-21 85.5
MS.gene96093.t1 AT5G37780 39.231 130 70 3 36 165 13 133 6.16e-21 83.6
MS.gene96093.t1 AT2G41410 36.552 145 86 2 21 165 69 207 6.65e-21 85.9
MS.gene96093.t1 AT1G66410 39.231 130 70 3 36 165 36 156 6.95e-21 84.3
MS.gene96093.t1 AT4G12860 35.135 148 87 3 22 168 5 144 8.17e-21 84.0
MS.gene96093.t1 AT3G50770 35.862 145 87 5 22 165 64 203 6.60e-20 82.8
MS.gene96093.t1 AT2G36180 35.616 146 88 3 25 170 3 142 2.93e-19 79.7
MS.gene96093.t1 AT1G66410 38.655 119 64 3 47 165 1 110 1.50e-18 77.0
MS.gene96093.t1 AT1G66410 38.655 119 64 3 47 165 1 110 1.50e-18 77.0
MS.gene96093.t1 AT2G41100 31.447 159 96 4 21 169 11 166 2.19e-18 80.9
MS.gene96093.t1 AT2G41100 31.410 156 93 4 21 165 100 252 4.64e-16 74.3
MS.gene96093.t1 AT2G41100 31.447 159 96 4 21 169 11 166 2.19e-18 80.9
MS.gene96093.t1 AT2G41100 31.410 156 93 4 21 165 100 252 4.64e-16 74.3
MS.gene96093.t1 AT1G18530 35.294 153 85 6 22 170 7 149 2.32e-18 77.8
MS.gene96093.t1 AT1G76640 32.099 162 102 5 5 166 6 159 2.38e-18 77.8
MS.gene96093.t1 AT3G50360 33.562 146 88 3 21 166 26 162 2.98e-18 77.8
MS.gene96093.t1 AT3G43810 38.655 119 64 3 47 165 1 110 4.72e-18 75.5
MS.gene96093.t1 AT3G43810 38.655 119 64 3 47 165 1 110 4.72e-18 75.5
MS.gene96093.t1 AT2G41090 34.000 150 86 4 21 169 11 148 6.62e-18 77.4
MS.gene96093.t1 AT4G14640 37.692 130 72 3 36 165 9 129 6.72e-18 75.9
MS.gene96093.t1 AT3G03400 36.090 133 78 3 26 158 9 134 1.53e-17 75.1
MS.gene96093.t1 AT4G20780 33.962 159 90 4 22 167 29 185 7.01e-17 74.7
MS.gene96093.t1 AT5G44460 38.562 153 83 5 22 167 28 176 1.18e-16 73.9
MS.gene96093.t1 AT3G10660 31.507 146 88 3 23 168 492 625 2.06e-16 76.3
MS.gene96093.t1 AT3G10660 31.507 146 88 3 23 168 492 625 2.06e-16 76.3
MS.gene96093.t1 AT2G41100 31.410 156 93 4 21 165 65 217 3.13e-16 74.3
MS.gene96093.t1 AT2G41100 31.410 156 93 4 21 165 65 217 3.13e-16 74.3
MS.gene96093.t1 AT2G41100 31.410 156 93 4 21 165 65 217 3.13e-16 74.3
MS.gene96093.t1 AT3G20410 33.125 160 94 4 10 168 384 531 4.15e-16 75.1
MS.gene96093.t1 AT3G20410 33.125 160 94 4 10 168 384 531 4.15e-16 75.1
MS.gene96093.t1 AT5G19360 31.507 146 89 3 23 168 374 508 4.79e-16 75.1
MS.gene96093.t1 AT1G76650 32.886 149 86 5 20 166 41 177 6.87e-16 71.6
MS.gene96093.t1 AT1G76650 32.886 149 86 5 20 166 41 177 6.87e-16 71.6
MS.gene96093.t1 AT4G04695 33.333 147 85 4 23 168 337 471 7.25e-16 74.3
MS.gene96093.t1 AT3G25600 32.000 150 94 4 22 170 12 154 1.35e-15 70.5
MS.gene96093.t1 AT4G23650 34.459 148 82 4 23 168 384 518 2.05e-15 73.2
MS.gene96093.t1 AT1G76040 34.014 147 84 4 23 168 179 313 2.78e-15 72.4
MS.gene96093.t1 AT1G76040 34.014 147 84 4 23 168 179 313 2.78e-15 72.4
MS.gene96093.t1 AT5G12180 30.822 146 90 3 23 168 379 513 2.96e-15 72.8
MS.gene96093.t1 AT1G76040 34.014 147 84 4 23 168 417 551 3.97e-15 72.4
MS.gene96093.t1 AT3G51850 31.325 166 104 3 5 170 342 497 4.83e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT4G04740 33.333 147 85 4 23 168 374 508 4.90e-15 72.0
MS.gene96093.t1 AT3G47480 33.628 113 64 1 67 168 69 181 5.05e-15 69.3
MS.gene96093.t1 AT1G50700 31.250 160 97 4 10 168 366 513 9.43e-15 71.2
MS.gene96093.t1 AT3G51920 29.412 153 98 4 13 165 4 146 1.13e-14 67.8
MS.gene96093.t1 AT5G23580 31.250 144 87 3 23 166 328 459 1.32e-14 70.9
MS.gene96093.t1 AT4G37010 31.034 145 91 3 22 166 27 162 1.45e-14 67.8
MS.gene96093.t1 AT4G37010 31.034 145 91 3 22 166 31 166 1.69e-14 67.8
MS.gene96093.t1 AT1G74740 34.228 149 86 4 23 170 365 502 1.89e-14 70.5
MS.gene96093.t1 AT5G12480 31.481 162 100 4 10 170 258 409 2.02e-14 70.5
MS.gene96093.t1 AT5G12480 31.481 162 100 4 10 170 352 503 2.32e-14 70.1
MS.gene96093.t1 AT2G41860 29.814 161 103 3 10 170 347 497 2.53e-14 70.1
MS.gene96093.t1 AT2G41860 29.814 161 103 3 10 170 347 497 2.65e-14 70.1
MS.gene96093.t1 AT4G21940 33.333 147 85 4 23 168 407 541 3.71e-14 69.7
MS.gene96093.t1 AT4G21940 33.333 147 85 4 23 168 407 541 4.22e-14 69.3
MS.gene96093.t1 AT2G38910 32.877 146 82 4 23 166 440 571 5.26e-14 69.3
MS.gene96093.t1 AT4G09570 32.192 146 87 3 23 168 331 464 5.36e-14 69.3
MS.gene96093.t1 AT4G04720 32.653 147 86 4 23 168 385 519 6.17e-14 68.9
MS.gene96093.t1 AT4G04720 32.653 147 86 4 23 168 385 519 6.17e-14 68.9
MS.gene96093.t1 AT4G04700 32.192 146 86 4 23 167 337 470 6.49e-14 68.9
MS.gene96093.t1 AT1G35670 31.507 146 88 3 23 168 332 465 1.27e-13 68.2
MS.gene96093.t1 AT1G18890 32.886 149 88 4 23 170 369 506 1.33e-13 68.2
MS.gene96093.t1 AT3G22930 36.190 105 59 2 22 126 35 131 2.75e-13 63.5
MS.gene96093.t1 AT3G57530 30.247 162 100 4 10 170 356 505 3.69e-13 66.6
MS.gene96093.t1 AT2G31500 31.757 148 90 4 25 170 374 512 4.06e-13 66.6
MS.gene96093.t1 AT2G17290 30.822 146 89 3 23 168 391 524 5.08e-13 66.2
MS.gene96093.t1 AT2G17290 30.822 146 89 3 23 168 391 524 5.08e-13 66.2
MS.gene96093.t1 AT4G35310 30.137 146 90 3 23 168 403 536 8.01e-13 65.9
MS.gene96093.t1 AT4G35310 30.137 146 90 3 23 168 403 536 8.01e-13 65.9
MS.gene96093.t1 AT5G04870 29.452 146 91 3 23 168 456 589 1.10e-12 65.5
MS.gene96093.t1 AT4G04710 31.507 146 89 3 23 168 324 458 2.18e-12 64.3
MS.gene96093.t1 AT4G04710 31.507 146 89 3 23 168 338 472 2.42e-12 64.3
MS.gene96093.t1 AT4G38230 30.822 146 89 3 23 168 186 319 3.05e-12 63.9
MS.gene96093.t1 AT4G04710 31.507 146 89 3 23 168 351 485 3.22e-12 63.9
MS.gene96093.t1 AT1G76650 31.544 149 78 6 20 166 41 167 3.47e-12 61.6
MS.gene96093.t1 AT4G38230 30.822 146 89 3 23 168 360 493 4.86e-12 63.5
MS.gene96093.t1 AT4G38230 30.822 146 89 3 23 168 330 463 5.01e-12 63.5
MS.gene96093.t1 AT4G38230 30.822 146 89 3 23 168 330 463 5.01e-12 63.5
MS.gene96093.t1 AT5G19450 29.814 161 104 3 10 170 350 501 5.34e-12 63.5
MS.gene96093.t1 AT5G19450 29.814 161 104 3 10 170 350 501 5.34e-12 63.5
MS.gene96093.t1 AT1G62820 29.054 148 88 4 21 165 12 145 8.51e-12 60.1
MS.gene96093.t1 AT1G12310 29.054 148 88 4 21 165 12 145 9.35e-12 60.1
MS.gene96093.t1 AT3G01830 27.891 147 95 4 21 166 10 146 1.65e-11 59.3
MS.gene96093.t1 AT3G03000 31.126 151 96 4 18 168 16 158 1.88e-11 59.7
MS.gene96093.t1 AT4G14640 34.286 105 61 2 21 125 12 108 3.22e-11 58.2
MS.gene96093.t1 AT4G04710 31.081 148 89 4 23 168 351 487 9.02e-11 59.7

Find 30 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGAGTTCTGATAAATTT+AGG 0.191580 7.4:-30111853 MS.gene96093:CDS
TGGAGATGGGTTTATAGATT+TGG 0.275317 7.4:-30112013 MS.gene96093:CDS
GATGATAAAGAAGGCAAGTT+TGG 0.295061 7.4:-30111970 MS.gene96093:CDS
TGAAGGTATGGTAAATGTTT+TGG 0.308363 7.4:-30112043 MS.gene96093:CDS
AGTGAGTTACTTTCATGTTT+GGG 0.342905 7.4:-30112096 MS.gene96093:CDS
AAGTGAGTTACTTTCATGTT+TGG 0.344677 7.4:-30112097 MS.gene96093:CDS
AAAGAAGAGAATATTGTGTT+AGG 0.360406 7.4:+30112207 None:intergenic
ATGTGGATTATGAAGAATTT+CGG 0.364481 7.4:-30111762 MS.gene96093:CDS
TATAGATTTGGAGGAGTTCA+TGG 0.367425 7.4:-30112001 MS.gene96093:CDS
GGAGGAGTTCATGGTTGTTA+TGG 0.370400 7.4:-30111992 MS.gene96093:CDS
TTCAAGCTGATTGACACTAA+TGG 0.380588 7.4:-30112147 MS.gene96093:CDS
GGATTTGATCATTGTAGTAT+TGG 0.395567 7.4:-30111832 MS.gene96093:CDS
TTTCGGTCTATGATGAAATC+AGG 0.445802 7.4:-30111745 MS.gene96093:CDS
GTCTTTGACACTGATAAGAA+TGG 0.445853 7.4:-30111898 MS.gene96093:CDS
AGATGGGTTTATAGATTTGG+AGG 0.449742 7.4:-30112010 MS.gene96093:CDS
ATCAAAGGGGTTGATAAGAA+TGG 0.475385 7.4:-30111793 MS.gene96093:CDS
GAATGGTTTAATTTCAGCTA+AGG 0.482153 7.4:-30111881 MS.gene96093:CDS
GGGGTTGATAAGAATGGAGA+TGG 0.483060 7.4:-30111787 MS.gene96093:CDS
AATGCAAACGTATGATCAAA+GGG 0.489788 7.4:-30111807 MS.gene96093:CDS
TGCTAAAGAAGCTGAAGGTA+TGG 0.497797 7.4:-30112055 MS.gene96093:CDS
GAATGCAAACGTATGATCAA+AGG 0.544401 7.4:-30111808 MS.gene96093:CDS
AAGTAACTCACTTAGCTCAC+TGG 0.558927 7.4:+30112107 None:intergenic
GTTGTTATGGATGATAAAGA+AGG 0.559166 7.4:-30111979 MS.gene96093:CDS
GGAGCAAGATGAGTATCTCA+TGG 0.564180 7.4:-30111932 MS.gene96093:CDS
CTGATTGACACTAATGGAGA+TGG 0.576539 7.4:-30112141 MS.gene96093:CDS
GTTTGGTTGTGCTAATGAGA+TGG 0.582850 7.4:-30111953 MS.gene96093:CDS
ATAGCTGCTAAAGAAGCTGA+AGG 0.611021 7.4:-30112060 MS.gene96093:CDS
TAAGAATGGAGATGGGTATG+TGG 0.620164 7.4:-30111779 MS.gene96093:CDS
GGGTTGATAAGAATGGAGAT+GGG 0.632436 7.4:-30111786 MS.gene96093:CDS
ATGCAAACGTATGATCAAAG+GGG 0.727254 7.4:-30111806 MS.gene96093:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAAGAGAATATTGTGTT+AGG + chr7.4:30111756-30111775 None:intergenic 25.0%
!! AAGAGAGTTCTGATAAATTT+AGG - chr7.4:30112107-30112126 MS.gene96093:CDS 25.0%
!! ATGTGGATTATGAAGAATTT+CGG - chr7.4:30112198-30112217 MS.gene96093:CDS 25.0%
!! ATTCTCTTCTTTTGTTGATA+TGG - chr7.4:30111764-30111783 MS.gene96093:CDS 25.0%
AATGCAAACGTATGATCAAA+GGG - chr7.4:30112153-30112172 MS.gene96093:CDS 30.0%
GGATTTGATCATTGTAGTAT+TGG - chr7.4:30112128-30112147 MS.gene96093:CDS 30.0%
GTTGTTATGGATGATAAAGA+AGG - chr7.4:30111981-30112000 MS.gene96093:CDS 30.0%
! AAGTGAGTTACTTTCATGTT+TGG - chr7.4:30111863-30111882 MS.gene96093:CDS 30.0%
! AGTGAGTTACTTTCATGTTT+GGG - chr7.4:30111864-30111883 MS.gene96093:CDS 30.0%
! GAATGGTTTAATTTCAGCTA+AGG - chr7.4:30112079-30112098 MS.gene96093:CDS 30.0%
!! GTAAATGTTTTGGACTCAAA+TGG - chr7.4:30111927-30111946 MS.gene96093:CDS 30.0%
!! TGAAGGTATGGTAAATGTTT+TGG - chr7.4:30111917-30111936 MS.gene96093:CDS 30.0%
ATGCAAACGTATGATCAAAG+GGG - chr7.4:30112154-30112173 MS.gene96093:CDS 35.0%
GAATGCAAACGTATGATCAA+AGG - chr7.4:30112152-30112171 MS.gene96093:CDS 35.0%
GATGATAAAGAAGGCAAGTT+TGG - chr7.4:30111990-30112009 MS.gene96093:CDS 35.0%
GTCTTTGACACTGATAAGAA+TGG - chr7.4:30112062-30112081 MS.gene96093:CDS 35.0%
TATAGATTTGGAGGAGTTCA+TGG - chr7.4:30111959-30111978 MS.gene96093:CDS 35.0%
TTCAAGCTGATTGACACTAA+TGG - chr7.4:30111813-30111832 MS.gene96093:CDS 35.0%
TTTCGGTCTATGATGAAATC+AGG - chr7.4:30112215-30112234 MS.gene96093:CDS 35.0%
! AGATGGGTTTATAGATTTGG+AGG - chr7.4:30111950-30111969 MS.gene96093:CDS 35.0%
! TGGAGATGGGTTTATAGATT+TGG - chr7.4:30111947-30111966 MS.gene96093:CDS 35.0%
! TTTTGGACTCAAATGGAGAT+GGG - chr7.4:30111934-30111953 MS.gene96093:CDS 35.0%
!! ATCAAAGGGGTTGATAAGAA+TGG - chr7.4:30112167-30112186 MS.gene96093:CDS 35.0%
AAGTAACTCACTTAGCTCAC+TGG + chr7.4:30111856-30111875 None:intergenic 40.0%
ATAGCTGCTAAAGAAGCTGA+AGG - chr7.4:30111900-30111919 MS.gene96093:CDS 40.0%
CTGATTGACACTAATGGAGA+TGG - chr7.4:30111819-30111838 MS.gene96093:CDS 40.0%
GTTTGGTTGTGCTAATGAGA+TGG - chr7.4:30112007-30112026 MS.gene96093:CDS 40.0%
TAAGAATGGAGATGGGTATG+TGG - chr7.4:30112181-30112200 MS.gene96093:CDS 40.0%
TGCTAAAGAAGCTGAAGGTA+TGG - chr7.4:30111905-30111924 MS.gene96093:CDS 40.0%
!! GGGTTGATAAGAATGGAGAT+GGG - chr7.4:30112174-30112193 MS.gene96093:CDS 40.0%
!! GTTTTGGACTCAAATGGAGA+TGG - chr7.4:30111933-30111952 MS.gene96093:CDS 40.0%
GGAGCAAGATGAGTATCTCA+TGG - chr7.4:30112028-30112047 MS.gene96093:CDS 45.0%
GGAGGAGTTCATGGTTGTTA+TGG - chr7.4:30111968-30111987 MS.gene96093:CDS 45.0%
!! GGGGTTGATAAGAATGGAGA+TGG - chr7.4:30112173-30112192 MS.gene96093:CDS 45.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 30111735 30112247 30111735 ID=MS.gene96093
chr7.4 mRNA 30111735 30112247 30111735 ID=MS.gene96093.t1;Parent=MS.gene96093
chr7.4 exon 30111735 30112247 30111735 ID=MS.gene96093.t1.exon1;Parent=MS.gene96093.t1
chr7.4 CDS 30111735 30112247 30111735 ID=cds.MS.gene96093.t1;Parent=MS.gene96093.t1
Gene Sequence

>MS.gene96093

ATGGACATTATAAGAGAACCTAACACAATATTCTCTTCTTTTGTTGATATGGAGATGTCTAATCAGTTCAAACAAGTTTTCAAGCTGATTGACACTAATGGAGATGGCAAGATATCAACCAGTGAGCTAAGTGAGTTACTTTCATGTTTGGGATGCAAAGATAGCATAGCTGCTAAAGAAGCTGAAGGTATGGTAAATGTTTTGGACTCAAATGGAGATGGGTTTATAGATTTGGAGGAGTTCATGGTTGTTATGGATGATAAAGAAGGCAAGTTTGGTTGTGCTAATGAGATGGAGCAAGATGAGTATCTCATGGATGCTTTTCATGTCTTTGACACTGATAAGAATGGTTTAATTTCAGCTAAGGAACTTAAGAGAGTTCTGATAAATTTAGGATTTGATCATTGTAGTATTGGAGAATGCAAACGTATGATCAAAGGGGTTGATAAGAATGGAGATGGGTATGTGGATTATGAAGAATTTCGGTCTATGATGAAATCAGGACAAGATTAG
Protein sequence

>MS.gene96093.t1

MDIIREPNTIFSSFVDMEMSNQFKQVFKLIDTNGDGKISTSELSELLSCLGCKDSIAAKEAEGMVNVLDSNGDGFIDLEEFMVVMDDKEGKFGCANEMEQDEYLMDAFHVFDTDKNGLISAKELKRVLINLGFDHCSIGECKRMIKGVDKNGDGYVDYEEFRSMMKSGQD