Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96172.t1 | ABN05850.1 | 93.8 | 64 | 4 | 0 | 17 | 80 | 63 | 126 | 6.70E-26 | 126.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96172.t1 | Q9ZPI1 | 49.3 | 69 | 35 | 0 | 21 | 89 | 85 | 153 | 4.3e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96172.t1 | G7IMZ7 | 93.8 | 64 | 4 | 0 | 17 | 80 | 63 | 126 | 4.8e-26 | 126.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049585 | MS.gene96172 | 0.810647 | 1.04E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene96172 | 0.810853 | 9.38E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene96172 | 0.80477 | 1.84E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene96172 | 0.833304 | 5.76E-56 | -1.69E-46 |
| MS.gene059439 | MS.gene96172 | 0.821433 | 4.05E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96172.t1 | MTR_2g036600 | 92.405 | 79 | 6 | 0 | 2 | 80 | 48 | 126 | 1.96e-45 | 154 |
| MS.gene96172.t1 | MTR_4g127770 | 64.557 | 79 | 27 | 1 | 2 | 80 | 535 | 612 | 3.41e-27 | 104 |
| MS.gene96172.t1 | MTR_2g021780 | 40.909 | 66 | 39 | 0 | 9 | 74 | 29 | 94 | 6.81e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96172.t1 | AT3G11710 | 47.059 | 85 | 45 | 0 | 2 | 86 | 66 | 150 | 5.60e-20 | 84.0 |
Find 30 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATACAAAACCCTTACATTT+TGG | 0.160225 | 5.1:+37347826 | None:intergenic |
| TCGAGACATTTAGGTATATT+AGG | 0.247416 | 5.1:-37347929 | MS.gene96172:intron |
| GCAGAGAATTTGTGTGGATA+TGG | 0.276532 | 5.1:+37348297 | None:intergenic |
| CATGCTTATGTTGCTCAGTA+TGG | 0.346734 | 5.1:-37347863 | MS.gene96172:CDS |
| CTGATGTTGGTAGTTATAGT+AGG | 0.373796 | 5.1:-37347889 | MS.gene96172:CDS |
| TGTTTAACTGCAGCAATACT+TGG | 0.390068 | 5.1:-37348367 | MS.gene96172:intron |
| CTGCATATGGCCAAAATGTA+AGG | 0.394148 | 5.1:-37347836 | None:intergenic |
| TGCAGCAATACTTGGAGAAC+AGG | 0.411482 | 5.1:-37348359 | MS.gene96172:intron |
| AGCTGCTGATGAGGAAGATA+TGG | 0.435435 | 5.1:-37348485 | MS.gene96172:CDS |
| CAGAGAATTTGTGTGGATAT+GGG | 0.448925 | 5.1:+37348298 | None:intergenic |
| GATGTTTCTGTATCTTTGAC+TGG | 0.451781 | 5.1:-37348210 | MS.gene96172:intron |
| TAAGTATCTTGCAACTGAAA+AGG | 0.466073 | 5.1:-37348334 | MS.gene96172:CDS |
| TCAGTATGGAGGTCTGCATA+TGG | 0.473519 | 5.1:-37347849 | MS.gene96172:CDS |
| AGGTATATTAGGTAGTTCTC+AGG | 0.485185 | 5.1:-37347918 | MS.gene96172:CDS |
| CTTGCAACTGAAAAGGAAGA+AGG | 0.491377 | 5.1:-37348327 | MS.gene96172:CDS |
| AGTATAGAGGTTTAAGTGAT+GGG | 0.496348 | 5.1:-37348245 | MS.gene96172:CDS |
| GATATATTGTTTAATAGTCA+TGG | 0.500652 | 5.1:+37348272 | None:intergenic |
| TCTTCCTCATCAGCAGCTGC+AGG | 0.513803 | 5.1:+37348490 | None:intergenic |
| CAGACCTGCAGCTGCTGATG+AGG | 0.516125 | 5.1:-37348494 | MS.gene96172:CDS |
| CAATATATCAAGGAGTATAG+AGG | 0.522228 | 5.1:-37348258 | MS.gene96172:CDS |
| GACTATTAAACAATATATCA+AGG | 0.525455 | 5.1:-37348268 | MS.gene96172:CDS |
| AGCTGCAGGTCTGTTCTCGT+TGG | 0.535724 | 5.1:+37348504 | None:intergenic |
| GAGTATAGAGGTTTAAGTGA+TGG | 0.539469 | 5.1:-37348246 | MS.gene96172:CDS |
| TTGCAACTGAAAAGGAAGAA+GGG | 0.547876 | 5.1:-37348326 | MS.gene96172:CDS |
| TGATGTTGGTAGTTATAGTA+GGG | 0.573304 | 5.1:-37347888 | MS.gene96172:CDS |
| GCTTATGTTGCTCAGTATGG+AGG | 0.574250 | 5.1:-37347860 | MS.gene96172:CDS |
| TCTCAGGAAGATGCTGATGT+TGG | 0.590847 | 5.1:-37347902 | MS.gene96172:CDS |
| TGCATATGGCCAAAATGTAA+GGG | 0.632254 | 5.1:-37347835 | None:intergenic |
| ATATTGTTTAATAGTCATGG+TGG | 0.712555 | 5.1:+37348275 | None:intergenic |
| ATGGTGGCAGAGAATTTGTG+TGG | 0.713727 | 5.1:+37348291 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATATCAAACATAAATTTTC+AGG | + | chr5.1:37347920-37347939 | None:intergenic | 15.0% |
| !! | GACTATTAAACAATATATCA+AGG | - | chr5.1:37348215-37348234 | MS.gene96172:CDS | 20.0% |
| !! | GATATATTGTTTAATAGTCA+TGG | + | chr5.1:37348214-37348233 | None:intergenic | 20.0% |
| !!! | TATAATGTCAATACTTTTCT+AGG | - | chr5.1:37347938-37347957 | MS.gene96172:intron | 20.0% |
| ! | ATATTGTTTAATAGTCATGG+TGG | + | chr5.1:37348211-37348230 | None:intergenic | 25.0% |
| ! | CTATAGAAGAAATAACATGT+TGG | + | chr5.1:37348063-37348082 | None:intergenic | 25.0% |
| !! | GAGATTGAAATTTTCTTCAT+TGG | + | chr5.1:37348457-37348476 | None:intergenic | 25.0% |
| !!! | ATGAACTGTTTTTTGTTACT+TGG | - | chr5.1:37348091-37348110 | MS.gene96172:intron | 25.0% |
| !!! | TGAACTGTTTTTTGTTACTT+GGG | - | chr5.1:37348092-37348111 | MS.gene96172:intron | 25.0% |
| AGTATAGAGGTTTAAGTGAT+GGG | - | chr5.1:37348238-37348257 | MS.gene96172:CDS | 30.0% | |
| ATTTAGCAGCAGAGTAAAAA+AGG | + | chr5.1:37348489-37348508 | None:intergenic | 30.0% | |
| TAAGTATCTTGCAACTGAAA+AGG | - | chr5.1:37348149-37348168 | MS.gene96172:intron | 30.0% | |
| TCGAGACATTTAGGTATATT+AGG | - | chr5.1:37348554-37348573 | MS.gene96172:intron | 30.0% | |
| TTCAGGAATTGAAATCAACA+CGG | + | chr5.1:37347903-37347922 | None:intergenic | 30.0% | |
| ! | CAATATATCAAGGAGTATAG+AGG | - | chr5.1:37348225-37348244 | MS.gene96172:CDS | 30.0% |
| ! | GTTTCAGAAAAAGAGTATGT+TGG | + | chr5.1:37348333-37348352 | None:intergenic | 30.0% |
| ! | TGAAATTTTCTTCATTGGCA+TGG | + | chr5.1:37348452-37348471 | None:intergenic | 30.0% |
| !! | CATTTAGTTTCTGCATGTTT+TGG | - | chr5.1:37347853-37347872 | MS.gene96172:CDS | 30.0% |
| !! | TGATGTTGGTAGTTATAGTA+GGG | - | chr5.1:37348595-37348614 | MS.gene96172:intron | 30.0% |
| AGGTATATTAGGTAGTTCTC+AGG | - | chr5.1:37348565-37348584 | MS.gene96172:intron | 35.0% | |
| ATAACATGTTGGAAACAACG+CGG | + | chr5.1:37348052-37348071 | None:intergenic | 35.0% | |
| CAGAGAATTTGTGTGGATAT+GGG | + | chr5.1:37348188-37348207 | None:intergenic | 35.0% | |
| GAGTATAGAGGTTTAAGTGA+TGG | - | chr5.1:37348237-37348256 | MS.gene96172:CDS | 35.0% | |
| TGTTTAACTGCAGCAATACT+TGG | - | chr5.1:37348116-37348135 | MS.gene96172:intron | 35.0% | |
| TTGCAACTGAAAAGGAAGAA+GGG | - | chr5.1:37348157-37348176 | MS.gene96172:intron | 35.0% | |
| ! | AGCAGAGTAAAAAAGGCATA+TGG | + | chr5.1:37348482-37348501 | None:intergenic | 35.0% |
| ! | GATGTTTCTGTATCTTTGAC+TGG | - | chr5.1:37348273-37348292 | MS.gene96172:CDS | 35.0% |
| !! | CTGATGTTGGTAGTTATAGT+AGG | - | chr5.1:37348594-37348613 | MS.gene96172:intron | 35.0% |
| AAAAAGAGGAGTCCTACTGT+TGG | + | chr5.1:37348026-37348045 | None:intergenic | 40.0% | |
| AAACAACGCGGTGAAAAAAG+AGG | + | chr5.1:37348040-37348059 | None:intergenic | 40.0% | |
| AGGTGTAGTGAAACAAAGCT+AGG | + | chr5.1:37348365-37348384 | None:intergenic | 40.0% | |
| CATGCTTATGTTGCTCAGTA+TGG | - | chr5.1:37348620-37348639 | MS.gene96172:intron | 40.0% | |
| CTTGCAACTGAAAAGGAAGA+AGG | - | chr5.1:37348156-37348175 | MS.gene96172:intron | 40.0% | |
| GAAGATATGGAACCAACAGT+AGG | - | chr5.1:37348011-37348030 | MS.gene96172:intron | 40.0% | |
| GCAGAGAATTTGTGTGGATA+TGG | + | chr5.1:37348189-37348208 | None:intergenic | 40.0% | |
| TGCTGTGATTCGAGACATTT+AGG | - | chr5.1:37348545-37348564 | MS.gene96172:intron | 40.0% | |
| AGCTGCTGATGAGGAAGATA+TGG | - | chr5.1:37347998-37348017 | MS.gene96172:intron | 45.0% | |
| ATGGTGGCAGAGAATTTGTG+TGG | + | chr5.1:37348195-37348214 | None:intergenic | 45.0% | |
| GCTTATGTTGCTCAGTATGG+AGG | - | chr5.1:37348623-37348642 | MS.gene96172:intron | 45.0% | |
| TCAGTATGGAGGTCTGCATA+TGG | - | chr5.1:37348634-37348653 | MS.gene96172:intron | 45.0% | |
| TCTCAGGAAGATGCTGATGT+TGG | - | chr5.1:37348581-37348600 | MS.gene96172:intron | 45.0% | |
| TGCAGCAATACTTGGAGAAC+AGG | - | chr5.1:37348124-37348143 | MS.gene96172:intron | 45.0% | |
| AGATGGAGAGAGAGAGAGAG+AGG | + | chr5.1:37348385-37348404 | None:intergenic | 50.0% | |
| GAGAGAGAGAGAGAGAGAGA+TGG | + | chr5.1:37348402-37348421 | None:intergenic | 50.0% | |
| AGCTGCAGGTCTGTTCTCGT+TGG | + | chr5.1:37347982-37348001 | None:intergenic | 55.0% | |
| TCTTCCTCATCAGCAGCTGC+AGG | + | chr5.1:37347996-37348015 | None:intergenic | 55.0% | |
| CAGACCTGCAGCTGCTGATG+AGG | - | chr5.1:37347989-37348008 | MS.gene96172:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 37347838 | 37348667 | 37347838 | ID=MS.gene96172 |
| chr5.1 | mRNA | 37347838 | 37348667 | 37347838 | ID=MS.gene96172.t1;Parent=MS.gene96172 |
| chr5.1 | exon | 37348665 | 37348667 | 37348665 | ID=MS.gene96172.t1.exon1;Parent=MS.gene96172.t1 |
| chr5.1 | CDS | 37348665 | 37348667 | 37348665 | ID=cds.MS.gene96172.t1;Parent=MS.gene96172.t1 |
| chr5.1 | exon | 37348477 | 37348545 | 37348477 | ID=MS.gene96172.t1.exon2;Parent=MS.gene96172.t1 |
| chr5.1 | CDS | 37348477 | 37348545 | 37348477 | ID=cds.MS.gene96172.t1;Parent=MS.gene96172.t1 |
| chr5.1 | exon | 37348211 | 37348376 | 37348211 | ID=MS.gene96172.t1.exon3;Parent=MS.gene96172.t1 |
| chr5.1 | CDS | 37348211 | 37348376 | 37348211 | ID=cds.MS.gene96172.t1;Parent=MS.gene96172.t1 |
| chr5.1 | exon | 37347838 | 37347938 | 37347838 | ID=MS.gene96172.t1.exon4;Parent=MS.gene96172.t1 |
| chr5.1 | CDS | 37347838 | 37347938 | 37347838 | ID=cds.MS.gene96172.t1;Parent=MS.gene96172.t1 |
| Gene Sequence |
| Protein sequence |
