AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96444

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96444.t1 MTR_6g051425 30.182 275 175 6 50 311 19 289 5.63e-35 136
MS.gene96444.t1 MTR_0856s0010 43.023 172 84 4 3 173 2 160 4.31e-33 125
MS.gene96444.t1 MTR_4g035820 30.405 296 180 12 9 288 39 324 9.16e-24 107
MS.gene96444.t1 MTR_0558s0020 25.952 289 180 8 30 284 8 296 1.11e-21 96.7
MS.gene96444.t1 MTR_1g070355 28.936 235 152 7 54 278 80 309 2.19e-20 96.3
MS.gene96444.t1 MTR_7g446060 51.220 82 40 0 130 211 7 88 5.73e-20 87.0
MS.gene96444.t1 MTR_3g450850 26.829 287 191 8 2 272 3 286 2.01e-17 85.1
MS.gene96444.t1 MTR_8g446020 24.419 258 173 5 34 284 14 256 2.87e-17 82.8
MS.gene96444.t1 MTR_7g446480 31.977 172 116 1 122 292 143 314 4.94e-17 83.2
MS.gene96444.t1 MTR_6g022070 28.966 145 99 1 338 482 1 141 5.27e-17 83.6
MS.gene96444.t1 MTR_1g008290 32.331 133 88 1 108 240 14 144 9.82e-17 78.2
MS.gene96444.t1 MTR_7g446510 32.530 166 111 1 128 292 164 329 1.93e-16 82.0
MS.gene96444.t1 MTR_7g446520 25.847 236 162 2 52 286 92 315 3.20e-16 81.3
MS.gene96444.t1 MTR_7g115320 61.111 54 18 1 137 190 9 59 8.75e-15 70.9
MS.gene96444.t1 MTR_1g083640 23.897 272 173 8 18 255 11 282 3.33e-14 74.3
MS.gene96444.t1 MTR_1g019850 29.697 165 99 6 17 175 43 196 1.23e-13 71.6
MS.gene96444.t1 MTR_3g012160 41.975 81 46 1 193 273 14 93 1.41e-13 67.4
MS.gene96444.t1 MTR_5g023310 28.662 157 43 3 179 334 16 104 2.94e-13 67.0
MS.gene96444.t1 MTR_5g076070 23.552 259 185 7 23 272 28 282 4.74e-13 70.9
MS.gene96444.t1 MTR_6g090505 25.843 178 129 2 109 283 53 230 2.20e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96444.t1 AT4G15090 31.744 734 460 13 48 771 51 753 1.48e-122 388
MS.gene96444.t1 AT4G15090 31.744 734 460 13 48 771 51 753 1.48e-122 388
MS.gene96444.t1 AT4G15090 31.744 734 460 13 48 771 51 753 1.48e-122 388
MS.gene96444.t1 AT4G15090 31.744 734 460 13 48 771 51 753 1.48e-122 388
MS.gene96444.t1 AT4G15090 31.744 734 460 13 48 771 51 753 1.48e-122 388
MS.gene96444.t1 AT4G38180 34.375 640 388 12 8 631 38 661 2.13e-119 378
MS.gene96444.t1 AT3G22170 29.910 779 470 14 18 770 35 763 7.77e-111 357
MS.gene96444.t1 AT3G22170 29.910 779 470 14 18 770 35 763 7.77e-111 357
MS.gene96444.t1 AT1G76320 32.198 587 374 9 52 633 1 568 4.68e-105 337
MS.gene96444.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.24e-104 338
MS.gene96444.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.27e-104 338
MS.gene96444.t1 AT1G76320 32.198 587 374 9 52 633 1 568 1.27e-104 338
MS.gene96444.t1 AT1G52520 32.538 587 371 12 33 607 66 639 5.69e-104 335
MS.gene96444.t1 AT2G27110 31.803 610 363 14 48 645 49 617 1.34e-93 311
MS.gene96444.t1 AT2G27110 31.803 610 363 14 48 645 49 617 1.34e-93 311
MS.gene96444.t1 AT3G06250 33.393 563 323 14 48 606 190 704 1.35e-92 306
MS.gene96444.t1 AT3G06250 33.393 563 323 14 48 606 190 704 1.35e-92 306
MS.gene96444.t1 AT3G06250 33.393 563 323 14 48 606 190 704 1.35e-92 306
MS.gene96444.t1 AT1G80010 32.392 602 359 13 39 610 82 665 1.62e-90 300
MS.gene96444.t1 AT2G27110 34.937 478 288 9 172 645 14 472 6.12e-89 295
MS.gene96444.t1 AT5G18960 32.060 602 354 16 28 622 192 745 6.62e-86 289
MS.gene96444.t1 AT5G18960 32.060 602 354 16 28 622 192 745 6.62e-86 289
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 135 564 3.85e-82 278
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 4.01e-82 278
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 135 564 4.21e-82 278
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 7.14e-82 279
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 7.14e-82 279
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 8.28e-82 278
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 8.28e-82 278
MS.gene96444.t1 AT2G32250 34.312 443 270 6 224 658 176 605 8.28e-82 278
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 24 583 3.44e-79 270
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 24 583 3.51e-79 270
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 24 583 3.51e-79 270
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 24 583 5.57e-79 270
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 24 583 5.57e-79 270
MS.gene96444.t1 AT4G19990 30.198 606 353 12 51 632 38 597 7.29e-79 270
MS.gene96444.t1 AT4G38170 34.021 388 245 4 221 606 21 399 3.09e-76 257
MS.gene96444.t1 AT4G19990 30.983 539 318 10 104 632 32 526 2.68e-75 258
MS.gene96444.t1 AT1G10240 26.433 628 413 17 1 603 1 604 5.20e-63 224
MS.gene96444.t1 AT1G10240 26.433 628 413 17 1 603 1 604 5.20e-63 224
MS.gene96444.t1 AT5G28530 27.946 594 364 13 46 603 53 618 1.54e-61 220
MS.gene96444.t1 AT5G28530 27.441 594 363 12 46 603 53 614 8.12e-61 218
MS.gene96444.t1 AT5G28530 27.441 594 363 12 46 603 53 614 8.12e-61 218
MS.gene96444.t1 AT5G28530 26.829 328 202 7 290 603 252 555 1.13e-27 119
MS.gene96444.t1 AT5G28530 25.915 328 201 6 290 603 252 551 6.81e-27 117

Find 156 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGTTGACGTTTCGGATTC+CGG 0.204382 1.2:+22471301 MS.gene96444:CDS
TCTTTCCCGCTCTTCGAAAA+TGG 0.249066 1.2:-22472463 None:intergenic
TAAGATGACCAAGTACAAAA+AGG 0.265908 1.2:-22472965 None:intergenic
GATTGCAAACCCTTGTTTCA+TGG 0.302647 1.2:-22471428 None:intergenic
AAGACCGCAACCTAGTAAAA+TGG 0.321194 1.2:-22472109 None:intergenic
GTGTTGACGTTTCGGATTCC+GGG 0.344560 1.2:+22471302 MS.gene96444:CDS
GGCTATGCAGAATGCAATTC+AGG 0.347065 1.2:+22472233 MS.gene96444:CDS
TTGCTTTAAAGAAGGGTCTA+TGG 0.361166 1.2:+22471599 MS.gene96444:CDS
TGCGGCTCGTGTTGACGTTT+CGG 0.362249 1.2:+22471294 MS.gene96444:CDS
ATGCCATTTGCCCCGTTTGT+TGG 0.369173 1.2:+22472066 MS.gene96444:CDS
ACTAAATCACGGTTGTTTGC+TGG 0.375082 1.2:+22471667 MS.gene96444:CDS
AGTATTGTGTTGATTTCAAC+GGG 0.389824 1.2:+22472877 MS.gene96444:CDS
CACCACCTATGCCTAGTATT+AGG 0.393202 1.2:-22472264 None:intergenic
GTACACCCATGACAAGTATA+TGG 0.398379 1.2:+22472731 MS.gene96444:CDS
GGATTAAGAATTGGTTGATC+AGG 0.405554 1.2:-22473299 None:intergenic
CTAGGCATAGGTGGTGTCTA+TGG 0.405638 1.2:+22472271 MS.gene96444:CDS
CCACACATTGAAAGATTTGA+AGG 0.409966 1.2:+22473092 MS.gene96444:CDS
ACTTCGACAACCGAAACAAT+TGG 0.413255 1.2:-22473419 None:intergenic
AAAGGATGATCGATCAATAT+AGG 0.415391 1.2:-22471241 None:intergenic
ACATTACTTCCTATGTCTGA+TGG 0.423977 1.2:+22473551 MS.gene96444:CDS
CCTCTGTGTTGTTGACATTC+CGG 0.424793 1.2:-22472520 None:intergenic
GATGATTGACACCAACAAAC+GGG 0.432575 1.2:-22472077 None:intergenic
TTGGTGGTATGTTTGATTCA+TGG 0.433399 1.2:-22471525 None:intergenic
ACACCAACAAACGGGGCAAA+TGG 0.438957 1.2:-22472069 None:intergenic
GCAAACAACCGTGATTTAGT+TGG 0.439208 1.2:-22471664 None:intergenic
ATGGTTTGGGTGAAAATGAT+TGG 0.445440 1.2:+22472433 MS.gene96444:CDS
CATTACTTCCTATGTCTGAT+GGG 0.447968 1.2:+22473552 MS.gene96444:CDS
TGATGATTGACACCAACAAA+CGG 0.449717 1.2:-22472078 None:intergenic
CCATCAGCTAGCTCTCCGTC+AGG 0.451558 1.2:-22471271 None:intergenic
ACACAATACTTAGTTTCGTT+CGG 0.457073 1.2:-22472864 None:intergenic
GGCTAAGGTGTATGTCTTCG+AGG 0.457503 1.2:+22472178 MS.gene96444:CDS
TATCCTACTTTGCTAGAATG+AGG 0.459924 1.2:+22471890 MS.gene96444:CDS
AAAACTAGAAACTCTAAGAA+AGG 0.460138 1.2:+22471451 MS.gene96444:CDS
AAACTAGAAACTCTAAGAAA+GGG 0.460938 1.2:+22471452 MS.gene96444:CDS
ACTCCAAGACAAGGCTGAAA+AGG 0.462412 1.2:+22472626 MS.gene96444:CDS
CAAATGGTCTACCAAGAAGT+TGG 0.463702 1.2:+22473196 MS.gene96444:CDS
AGGTTGTGTTTCCTAATACT+AGG 0.465284 1.2:+22472253 MS.gene96444:CDS
GCAAAAGAAAGACACCTTCT+CGG 0.472868 1.2:+22471841 MS.gene96444:CDS
GATAACGGTTGCTTTAAAGA+AGG 0.473052 1.2:+22471591 MS.gene96444:CDS
AACATCACCAAAGTATTCAT+AGG 0.473828 1.2:-22472004 None:intergenic
TTGTGTGTATGATTCTTTGC+GGG 0.475077 1.2:+22472368 MS.gene96444:CDS
TTTGTTGGTGTCAATCATCA+TGG 0.475674 1.2:+22472081 MS.gene96444:CDS
GCCACATTGTGGTGTGGGAG+GGG 0.479684 1.2:+22473235 MS.gene96444:CDS
GAGCAATCAAGTTTCTTCGT+TGG 0.480101 1.2:-22471562 None:intergenic
CACTTATGCTCGATGGCTGA+TGG 0.482201 1.2:+22471214 None:intergenic
TCGGGAAGGAAGGAGATGGC+AGG 0.482875 1.2:+22471860 MS.gene96444:CDS
ATTTGTTGTTTCAAAGGTCC+CGG 0.485747 1.2:-22471320 None:intergenic
CCTTCAAATCTTTCAATGTG+TGG 0.491616 1.2:-22473092 None:intergenic
GACAGCATTTGTTGTTTCAA+AGG 0.491833 1.2:-22471326 None:intergenic
CTTGTTTCATGGCATATTCA+CGG 0.492232 1.2:-22471417 None:intergenic
AAAACCACTAGTTGACTGTT+GGG 0.492621 1.2:-22473612 None:intergenic
TCCGTTAAAAGTACCACGTA+AGG 0.492742 1.2:+22473319 MS.gene96444:CDS
GACAATACTCTCCTCTAAAA+CGG 0.498094 1.2:-22472832 None:intergenic
CGTGAATATGCCATGAAACA+AGG 0.498225 1.2:+22471418 MS.gene96444:CDS
TGTATGAAGAAATGATCAAA+TGG 0.501577 1.2:+22473180 MS.gene96444:CDS
TTAAAAGACATACCGATAGT+AGG 0.501742 1.2:-22471367 None:intergenic
AAATGGTCTACCAAGAAGTT+GGG 0.502052 1.2:+22473197 MS.gene96444:CDS
TTTCGAAGAGCGGGAAAGAT+GGG 0.502201 1.2:+22472467 MS.gene96444:CDS
GTTGGATTCGTCTTCAAAGT+TGG 0.502236 1.2:-22471544 None:intergenic
ATACTTGTCATGGGTGTACT+CGG 0.502362 1.2:-22472727 None:intergenic
CATCTCCTTCCTTCCCGAGA+AGG 0.502837 1.2:-22471855 None:intergenic
TTACCTGGTTGTTCGAAACA+TGG 0.503284 1.2:+22472157 MS.gene96444:CDS
TGGTCATCTTAGAGTGAAAG+AGG 0.503540 1.2:+22472977 MS.gene96444:CDS
TATGTCTTCGAGGCCTCCAA+TGG 0.508338 1.2:+22472188 MS.gene96444:CDS
TCGGTTACAATTCCCATTGG+AGG 0.511504 1.2:-22472201 None:intergenic
TCTGATGGGTTTCAAGCGTC+CGG 0.512219 1.2:+22473566 MS.gene96444:CDS
TTACGAAAATCTAACATTTG+TGG 0.512305 1.2:+22471795 MS.gene96444:CDS
AAACGATCAGGCAGGTGTAC+GGG 0.512319 1.2:+22471732 MS.gene96444:CDS
CGTTCCATGGTATGTGATGC+CGG 0.512631 1.2:+22471769 MS.gene96444:CDS
GAGAGATGTCAGAAACTTTG+TGG 0.514693 1.2:+22471819 MS.gene96444:CDS
TCTGGGCTGATGCACGTAGT+AGG 0.516810 1.2:+22471977 MS.gene96444:CDS
CAAAAGAAAGACACCTTCTC+GGG 0.517286 1.2:+22471842 MS.gene96444:CDS
GTATCTCCATATACTTGTCA+TGG 0.517305 1.2:-22472737 None:intergenic
ATAACGGTTGCTTTAAAGAA+GGG 0.522275 1.2:+22471592 MS.gene96444:CDS
TTCCCTCATTCTAGCAAAGT+AGG 0.524536 1.2:-22471893 None:intergenic
GAACAAAAGTTTCCGTTCCA+TGG 0.529264 1.2:+22471756 MS.gene96444:CDS
GGATTCGTCTTCAAAGTTGG+TGG 0.530111 1.2:-22471541 None:intergenic
TAAACGATCAGGCAGGTGTA+CGG 0.533293 1.2:+22471731 MS.gene96444:CDS
AAGTATTGTGTTGATTTCAA+CGG 0.536214 1.2:+22472876 MS.gene96444:CDS
TAAAACCACTAGTTGACTGT+TGG 0.536849 1.2:-22473613 None:intergenic
ATTGTTGAATTTGACCCGCA+TGG 0.537156 1.2:-22472687 None:intergenic
ATCCTACTTTGCTAGAATGA+GGG 0.537508 1.2:+22471891 MS.gene96444:CDS
TGATCGGTTACAATTCCCAT+TGG 0.541217 1.2:-22472204 None:intergenic
CATCAGCTAGCTCTCCGTCA+GGG 0.541329 1.2:-22471270 None:intergenic
CTGCATAGCCTTGCACTGAT+CGG 0.542185 1.2:-22472220 None:intergenic
CTATGAGATTGACATGGACG+AGG 0.547341 1.2:+22471933 MS.gene96444:CDS
TGTTTGTTGTTTGAGTTCAG+AGG 0.550114 1.2:+22472924 MS.gene96444:CDS
TGATTGCTCAGCTAAGATAA+CGG 0.550952 1.2:+22471576 MS.gene96444:CDS
GATAAATTCACGCCTACTAT+CGG 0.551302 1.2:+22471355 MS.gene96444:CDS
TATCTCCATATACTTGTCAT+GGG 0.551454 1.2:-22472736 None:intergenic
ATTCTAACTTGTGCAAGAGA+AGG 0.552020 1.2:+22471499 MS.gene96444:CDS
AGGTCTGCCTATGAATACTT+TGG 0.552277 1.2:+22471997 MS.gene96444:CDS
AGACACCTTCTCGGGAAGGA+AGG 0.553011 1.2:+22471850 MS.gene96444:CDS
CAGCCACATTGTGGTGTGGG+AGG 0.557227 1.2:+22473233 MS.gene96444:CDS
TTACAGCCACATTGTGGTGT+GGG 0.557892 1.2:+22473230 MS.gene96444:CDS
TGTTTCGAACAACCAGGTAA+AGG 0.558836 1.2:-22472154 None:intergenic
AACTCTAAGAAAGGGGCCGA+TGG 0.560180 1.2:+22471460 MS.gene96444:CDS
AGCCACATTGTGGTGTGGGA+GGG 0.562336 1.2:+22473234 MS.gene96444:CDS
CTAAATTTATCTTGCCACGA+TGG 0.563368 1.2:+22473006 MS.gene96444:CDS
GAGATACAAGTAGAATTTCG+TGG 0.566723 1.2:+22472753 MS.gene96444:CDS
GAACAACCAGGTAAAGGACT+TGG 0.570795 1.2:-22472148 None:intergenic
ATGTCTTCGAGGCCTCCAAT+GGG 0.571048 1.2:+22472189 MS.gene96444:CDS
TGTTTCCTAATACTAGGCAT+AGG 0.571109 1.2:+22472259 MS.gene96444:CDS
TTGTTGGTGTCAATCATCAT+GGG 0.576932 1.2:+22472082 MS.gene96444:CDS
TCTCGAAGGTGTGACTACAT+TGG 0.577648 1.2:+22472791 MS.gene96444:CDS
ATCAGCTAGCTCTCCGTCAG+GGG 0.582853 1.2:-22471269 None:intergenic
TCAGACATAGGAAGTAATGT+AGG 0.589454 1.2:-22473548 None:intergenic
CATTTCTTCATACAAATCAG+AGG 0.596809 1.2:-22473171 None:intergenic
GCTTGAAACCCATCAGACAT+AGG 0.602294 1.2:-22473560 None:intergenic
GTGAATATGCCATGAAACAA+GGG 0.602322 1.2:+22471419 MS.gene96444:CDS
AAGATACCAAGTCCTTTACC+TGG 0.605756 1.2:+22472142 MS.gene96444:CDS
GAAGTAGTTAAGCGTATCAC+CGG 0.605920 1.2:-22473585 None:intergenic
CGACAACGCACTCCAAGACA+AGG 0.606607 1.2:+22472617 MS.gene96444:CDS
AGATACAAGTAGAATTTCGT+GGG 0.607840 1.2:+22472754 MS.gene96444:CDS
AGAAAGACACCTTCTCGGGA+AGG 0.609091 1.2:+22471846 MS.gene96444:CDS
GACAACCGACGATTAGTTCG+AGG 0.611005 1.2:-22473347 None:intergenic
TGCATCAATGAGCAAAGATG+TGG 0.611914 1.2:+22473646 MS.gene96444:CDS
ACCACCGGCATCACATACCA+TGG 0.613048 1.2:-22471773 None:intergenic
AAATGCAACTGTTATCTCGA+AGG 0.615193 1.2:+22472777 MS.gene96444:CDS
TTCCTAATACTAGGCATAGG+TGG 0.615209 1.2:+22472262 MS.gene96444:CDS
CAAGCACAATCATTCCATGC+GGG 0.616971 1.2:+22472673 MS.gene96444:CDS
GGTTGTTCGAAACATGGCTA+AGG 0.617470 1.2:+22472163 MS.gene96444:CDS
CGTGGCAAGATAAATTTAGA+CGG 0.619992 1.2:-22473002 None:intergenic
AATTGTAACCGATCAGTGCA+AGG 0.620761 1.2:+22472212 MS.gene96444:CDS
CGGGAAGGAAGGAGATGGCA+GGG 0.622349 1.2:+22471861 MS.gene96444:CDS
TCATCCCAACAGTCAACTAG+TGG 0.623204 1.2:+22473608 MS.gene96444:CDS
CCTGACGGAGAGCTAGCTGA+TGG 0.623440 1.2:+22471271 MS.gene96444:CDS
CCGTTAAAAGTACCACGTAA+GGG 0.624486 1.2:+22473320 MS.gene96444:CDS
CGGCATCACATACCATGGAA+CGG 0.625469 1.2:-22471768 None:intergenic
ATTGTGTGTATGATTCTTTG+CGG 0.625780 1.2:+22472367 MS.gene96444:CDS
TAGCCATGTTTCGAACAACC+AGG 0.627145 1.2:-22472160 None:intergenic
GTTCGAGGACGTCCCTTACG+TGG 0.628497 1.2:-22473332 None:intergenic
CTTCTCGGGAAGGAAGGAGA+TGG 0.631777 1.2:+22471856 MS.gene96444:CDS
TTTACAGCCACATTGTGGTG+TGG 0.632168 1.2:+22473229 MS.gene96444:CDS
AACTAGAAACTCTAAGAAAG+GGG 0.633083 1.2:+22471453 MS.gene96444:CDS
ATGAGTTTACAGCCACATTG+TGG 0.634509 1.2:+22473224 MS.gene96444:CDS
AAAGGATACGCACAATACAA+AGG 0.637659 1.2:+22472324 MS.gene96444:CDS
CAAGGCTGAAAAGGAATACG+AGG 0.638254 1.2:+22472635 MS.gene96444:CDS
GGATGATCGATCAATATAGG+AGG 0.639611 1.2:-22471238 None:intergenic
TACAAATCAGAGGTTTGACT+AGG 0.642589 1.2:-22473161 None:intergenic
AGATAAGTCCAACTAAATCA+CGG 0.645312 1.2:+22471656 MS.gene96444:CDS
TCAGCTAGCTCTCCGTCAGG+GGG 0.662037 1.2:-22471268 None:intergenic
TTATGAAATTGCAGAATGTG+CGG 0.662542 1.2:+22473130 MS.gene96444:CDS
CGGAGAGCTAGCTGATGGTG+CGG 0.662675 1.2:+22471276 MS.gene96444:CDS
ATGATTGACACCAACAAACG+GGG 0.665562 1.2:-22472076 None:intergenic
TTAGAGGAGAGTATTGTCTG+TGG 0.669312 1.2:+22472837 MS.gene96444:CDS
GGATCATAGCTTAAACACGA+TGG 0.674970 1.2:+22473256 MS.gene96444:CDS
GACGTCCTCGAACTAATCGT+CGG 0.680634 1.2:+22473342 MS.gene96444:CDS
TCATAGCTTAAACACGATGG+AGG 0.690849 1.2:+22473259 MS.gene96444:CDS
ATACTTGTTTGTCAAGTACG+TGG 0.696002 1.2:-22472040 None:intergenic
TCAAGCACAATCATTCCATG+CGG 0.697666 1.2:+22472672 MS.gene96444:CDS
TCCCCTCCCACACCACAATG+TGG 0.710147 1.2:-22473236 None:intergenic
TCCATGGTATGTGATGCCGG+TGG 0.764195 1.2:+22471772 MS.gene96444:CDS
CCGGAATGTCAACAACACAG+AGG 0.797075 1.2:+22472520 MS.gene96444:CDS
AGATTGATATGCATGCACAG+CGG 0.798983 1.2:+22471698 MS.gene96444:CDS
GTATTGTGTTGATTTCAACG+GGG 0.841514 1.2:+22472878 MS.gene96444:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAAAAAATGATTTCT+GGG + chr1.2:22472497-22472516 MS.gene96444:CDS 10.0%
!!! AAATCATTTTTTAAATAACA+TGG - chr1.2:22472495-22472514 None:intergenic 10.0%
!! AATGTAATCACAATAATAAT+AGG - chr1.2:22473458-22473477 None:intergenic 15.0%
!! GTTATTTAAAAAATGATTTC+TGG + chr1.2:22472496-22472515 MS.gene96444:CDS 15.0%
!! AAGAAAAAAACCAATTGTTT+CGG + chr1.2:22473409-22473428 MS.gene96444:CDS 20.0%
!! ACAACATTCATTGAAAAATA+TGG + chr1.2:22472414-22472433 MS.gene96444:CDS 20.0%
!! ATTCATTGAAAAATATGGTT+TGG + chr1.2:22472419-22472438 MS.gene96444:CDS 20.0%
!! TTCATTGAAAAATATGGTTT+GGG + chr1.2:22472420-22472439 MS.gene96444:CDS 20.0%
! AAAATTGCCGAAAAATTGAA+AGG + chr1.2:22472306-22472325 MS.gene96444:CDS 25.0%
! TGTATGAAGAAATGATCAAA+TGG + chr1.2:22473180-22473199 MS.gene96444:CDS 25.0%
! TTACGAAAATCTAACATTTG+TGG + chr1.2:22471795-22471814 MS.gene96444:CDS 25.0%
!! AAAACTAGAAACTCTAAGAA+AGG + chr1.2:22471451-22471470 MS.gene96444:CDS 25.0%
!! AAACTAGAAACTCTAAGAAA+GGG + chr1.2:22471452-22471471 MS.gene96444:CDS 25.0%
!! TATCGGTATGTCTTTTAAAT+CGG + chr1.2:22471372-22471391 MS.gene96444:CDS 25.0%
!!! AAGTATTGTGTTGATTTCAA+CGG + chr1.2:22472876-22472895 MS.gene96444:CDS 25.0%
!!! ACTTTTAACGGATTAAGAAT+TGG - chr1.2:22473311-22473330 None:intergenic 25.0%
!!! GAAATATTTTAACATGCCAT+CGG - chr1.2:22471479-22471498 None:intergenic 25.0%
!!! TTTTTTCACAATCTTTTCAG+TGG - chr1.2:22473375-22473394 None:intergenic 25.0%
AAAGGATGATCGATCAATAT+AGG - chr1.2:22471244-22471263 None:intergenic 30.0%
AAGATGACCAAGTACAAAAA+GGG - chr1.2:22472967-22472986 None:intergenic 30.0%
ACACAATACTTAGTTTCGTT+CGG - chr1.2:22472867-22472886 None:intergenic 30.0%
AGATAAGTCCAACTAAATCA+CGG + chr1.2:22471656-22471675 MS.gene96444:CDS 30.0%
AGATACAAGTAGAATTTCGT+GGG + chr1.2:22472754-22472773 MS.gene96444:CDS 30.0%
ATTGTGTGTATGATTCTTTG+CGG + chr1.2:22472367-22472386 MS.gene96444:CDS 30.0%
CATTTCTTCATACAAATCAG+AGG - chr1.2:22473174-22473193 None:intergenic 30.0%
TAAGATGACCAAGTACAAAA+AGG - chr1.2:22472968-22472987 None:intergenic 30.0%
TATCTCCATATACTTGTCAT+GGG - chr1.2:22472739-22472758 None:intergenic 30.0%
TTAAAAGACATACCGATAGT+AGG - chr1.2:22471370-22471389 None:intergenic 30.0%
TTATGAAATTGCAGAATGTG+CGG + chr1.2:22473130-22473149 MS.gene96444:CDS 30.0%
! AACATCACCAAAGTATTCAT+AGG - chr1.2:22472007-22472026 None:intergenic 30.0%
! AACTAGAAACTCTAAGAAAG+GGG + chr1.2:22471453-22471472 MS.gene96444:CDS 30.0%
! CTTTTTCTATGAGATTGACA+TGG + chr1.2:22471927-22471946 MS.gene96444:CDS 30.0%
! TCATGTGCAGAATATTTTCT+GGG + chr1.2:22471960-22471979 MS.gene96444:CDS 30.0%
! TGCGTATCCTTTCAATTTTT+CGG - chr1.2:22472316-22472335 None:intergenic 30.0%
! TTAAAAAATGATTTCTGGGC+CGG + chr1.2:22472501-22472520 MS.gene96444:CDS 30.0%
! TTCATGTGCAGAATATTTTC+TGG + chr1.2:22471959-22471978 MS.gene96444:CDS 30.0%
!! AGTATTGTGTTGATTTCAAC+GGG + chr1.2:22472877-22472896 MS.gene96444:CDS 30.0%
!! ATAACGGTTGCTTTAAAGAA+GGG + chr1.2:22471592-22471611 MS.gene96444:CDS 30.0%
!! GACAATTTTGATAAACGATC+AGG + chr1.2:22471720-22471739 MS.gene96444:CDS 30.0%
!!! AATGTGTGGTTGTAGTTTTT+TGG - chr1.2:22473081-22473100 None:intergenic 30.0%
!!! AGTATGAATGCTTTTTTCGA+TGG + chr1.2:22472549-22472568 MS.gene96444:CDS 30.0%
AAAACCACTAGTTGACTGTT+GGG - chr1.2:22473615-22473634 None:intergenic 35.0%
AAAGGATACGCACAATACAA+AGG + chr1.2:22472324-22472343 MS.gene96444:CDS 35.0%
AAATGCAACTGTTATCTCGA+AGG + chr1.2:22472777-22472796 MS.gene96444:CDS 35.0%
AAATGGTCTACCAAGAAGTT+GGG + chr1.2:22473197-22473216 MS.gene96444:CDS 35.0%
ACATTACTTCCTATGTCTGA+TGG + chr1.2:22473551-22473570 MS.gene96444:CDS 35.0%
AGGTTGTGTTTCCTAATACT+AGG + chr1.2:22472253-22472272 MS.gene96444:CDS 35.0%
ATACTTGTTTGTCAAGTACG+TGG - chr1.2:22472043-22472062 None:intergenic 35.0%
ATCCTACTTTGCTAGAATGA+GGG + chr1.2:22471891-22471910 MS.gene96444:CDS 35.0%
CATTACTTCCTATGTCTGAT+GGG + chr1.2:22473552-22473571 MS.gene96444:CDS 35.0%
CCACACATTGAAAGATTTGA+AGG + chr1.2:22473092-22473111 MS.gene96444:CDS 35.0%
CCTTCAAATCTTTCAATGTG+TGG - chr1.2:22473095-22473114 None:intergenic 35.0%
CGTGGCAAGATAAATTTAGA+CGG - chr1.2:22473005-22473024 None:intergenic 35.0%
CTAAATTTATCTTGCCACGA+TGG + chr1.2:22473006-22473025 MS.gene96444:CDS 35.0%
CTTGTTTCATGGCATATTCA+CGG - chr1.2:22471420-22471439 None:intergenic 35.0%
GAGATACAAGTAGAATTTCG+TGG + chr1.2:22472753-22472772 MS.gene96444:CDS 35.0%
GATAAATTCACGCCTACTAT+CGG + chr1.2:22471355-22471374 MS.gene96444:CDS 35.0%
GGATTAAGAATTGGTTGATC+AGG - chr1.2:22473302-22473321 None:intergenic 35.0%
GTATCTCCATATACTTGTCA+TGG - chr1.2:22472740-22472759 None:intergenic 35.0%
GTGAATATGCCATGAAACAA+GGG + chr1.2:22471419-22471438 MS.gene96444:CDS 35.0%
TAAAACCACTAGTTGACTGT+TGG - chr1.2:22473616-22473635 None:intergenic 35.0%
TACAAATCAGAGGTTTGACT+AGG - chr1.2:22473164-22473183 None:intergenic 35.0%
TATCCTACTTTGCTAGAATG+AGG + chr1.2:22471890-22471909 MS.gene96444:CDS 35.0%
TCAGACATAGGAAGTAATGT+AGG - chr1.2:22473551-22473570 None:intergenic 35.0%
TGATGATTGACACCAACAAA+CGG - chr1.2:22472081-22472100 None:intergenic 35.0%
TGATTGCTCAGCTAAGATAA+CGG + chr1.2:22471576-22471595 MS.gene96444:CDS 35.0%
TGTTTCCTAATACTAGGCAT+AGG + chr1.2:22472259-22472278 MS.gene96444:CDS 35.0%
TTGTGTGTATGATTCTTTGC+GGG + chr1.2:22472368-22472387 MS.gene96444:CDS 35.0%
! ATCTACATACACCGTTTTAG+AGG + chr1.2:22472821-22472840 MS.gene96444:CDS 35.0%
! ATGGTTTGGGTGAAAATGAT+TGG + chr1.2:22472433-22472452 MS.gene96444:CDS 35.0%
! ATTCTAACTTGTGCAAGAGA+AGG + chr1.2:22471499-22471518 MS.gene96444:CDS 35.0%
! CAATCCATTTTACTAGGTTG+CGG + chr1.2:22472105-22472124 MS.gene96444:CDS 35.0%
! GACAATACTCTCCTCTAAAA+CGG - chr1.2:22472835-22472854 None:intergenic 35.0%
! TGAATACCATTTTCGAAGAG+CGG + chr1.2:22472457-22472476 MS.gene96444:CDS 35.0%
! TGTTAGATTTTCGTAACCAC+CGG - chr1.2:22471791-22471810 None:intergenic 35.0%
! TTCACATTTTTACTCCATCG+TGG - chr1.2:22473023-22473042 None:intergenic 35.0%
! TTGGTGGTATGTTTGATTCA+TGG - chr1.2:22471528-22471547 None:intergenic 35.0%
! TTTCACAATCTTTTCAGTGG+TGG - chr1.2:22473372-22473391 None:intergenic 35.0%
!! ATTTGTTGTTTCAAAGGTCC+CGG - chr1.2:22471323-22471342 None:intergenic 35.0%
!! ATTTTGATAAACGATCAGGC+AGG + chr1.2:22471724-22471743 MS.gene96444:CDS 35.0%
!! GACAGCATTTGTTGTTTCAA+AGG - chr1.2:22471329-22471348 None:intergenic 35.0%
!! GATAACGGTTGCTTTAAAGA+AGG + chr1.2:22471591-22471610 MS.gene96444:CDS 35.0%
!! GTATTGTGTTGATTTCAACG+GGG + chr1.2:22472878-22472897 MS.gene96444:CDS 35.0%
!! TGTTTGTTGTTTGAGTTCAG+AGG + chr1.2:22472924-22472943 MS.gene96444:CDS 35.0%
!! TTGCTTTAAAGAAGGGTCTA+TGG + chr1.2:22471599-22471618 MS.gene96444:CDS 35.0%
!! TTGTTGGTGTCAATCATCAT+GGG + chr1.2:22472082-22472101 MS.gene96444:CDS 35.0%
!! TTTGTTGGTGTCAATCATCA+TGG + chr1.2:22472081-22472100 MS.gene96444:CDS 35.0%
!!! GGGTTGATGATTTTGTTGTT+GGG + chr1.2:22472388-22472407 MS.gene96444:CDS 35.0%
!!! TTGATGATTTTGTTGTTGGG+TGG + chr1.2:22472391-22472410 MS.gene96444:CDS 35.0%
AAGACCGCAACCTAGTAAAA+TGG - chr1.2:22472112-22472131 None:intergenic 40.0%
AAGATACCAAGTCCTTTACC+TGG + chr1.2:22472142-22472161 MS.gene96444:CDS 40.0%
AATTGTAACCGATCAGTGCA+AGG + chr1.2:22472212-22472231 MS.gene96444:CDS 40.0%
ACTAAATCACGGTTGTTTGC+TGG + chr1.2:22471667-22471686 MS.gene96444:CDS 40.0%
ACTTCGACAACCGAAACAAT+TGG - chr1.2:22473422-22473441 None:intergenic 40.0%
AGATTGATATGCATGCACAG+CGG + chr1.2:22471698-22471717 MS.gene96444:CDS 40.0%
AGGTCTGCCTATGAATACTT+TGG + chr1.2:22471997-22472016 MS.gene96444:CDS 40.0%
ATACTTGTCATGGGTGTACT+CGG - chr1.2:22472730-22472749 None:intergenic 40.0%
ATGAGTTTACAGCCACATTG+TGG + chr1.2:22473224-22473243 MS.gene96444:CDS 40.0%
ATGATTGACACCAACAAACG+GGG - chr1.2:22472079-22472098 None:intergenic 40.0%
ATTGTTGAATTTGACCCGCA+TGG - chr1.2:22472690-22472709 None:intergenic 40.0%
CAAAAGAAAGACACCTTCTC+GGG + chr1.2:22471842-22471861 MS.gene96444:CDS 40.0%
CAAATGGTCTACCAAGAAGT+TGG + chr1.2:22473196-22473215 MS.gene96444:CDS 40.0%
CGTGAATATGCCATGAAACA+AGG + chr1.2:22471418-22471437 MS.gene96444:CDS 40.0%
GAACAAAAGTTTCCGTTCCA+TGG + chr1.2:22471756-22471775 MS.gene96444:CDS 40.0%
GAAGTAGTTAAGCGTATCAC+CGG - chr1.2:22473588-22473607 None:intergenic 40.0%
GAGAGATGTCAGAAACTTTG+TGG + chr1.2:22471819-22471838 MS.gene96444:CDS 40.0%
GAGCAATCAAGTTTCTTCGT+TGG - chr1.2:22471565-22471584 None:intergenic 40.0%
GATGATTGACACCAACAAAC+GGG - chr1.2:22472080-22472099 None:intergenic 40.0%
GCAAAAGAAAGACACCTTCT+CGG + chr1.2:22471841-22471860 MS.gene96444:CDS 40.0%
GCAAACAACCGTGATTTAGT+TGG - chr1.2:22471667-22471686 None:intergenic 40.0%
GGATCATAGCTTAAACACGA+TGG + chr1.2:22473256-22473275 MS.gene96444:CDS 40.0%
GGATGATCGATCAATATAGG+AGG - chr1.2:22471241-22471260 None:intergenic 40.0%
GTACACCCATGACAAGTATA+TGG + chr1.2:22472731-22472750 MS.gene96444:CDS 40.0%
GTTGGATTCGTCTTCAAAGT+TGG - chr1.2:22471547-22471566 None:intergenic 40.0%
TCAAGCACAATCATTCCATG+CGG + chr1.2:22472672-22472691 MS.gene96444:CDS 40.0%
TCATAGCTTAAACACGATGG+AGG + chr1.2:22473259-22473278 MS.gene96444:CDS 40.0%
TGATCGGTTACAATTCCCAT+TGG - chr1.2:22472207-22472226 None:intergenic 40.0%
TGCATCAATGAGCAAAGATG+TGG + chr1.2:22473646-22473665 MS.gene96444:CDS 40.0%
TGGTCATCTTAGAGTGAAAG+AGG + chr1.2:22472977-22472996 MS.gene96444:CDS 40.0%
TGTTTCGAACAACCAGGTAA+AGG - chr1.2:22472157-22472176 None:intergenic 40.0%
TTACCTGGTTGTTCGAAACA+TGG + chr1.2:22472157-22472176 MS.gene96444:CDS 40.0%
TTCCCTCATTCTAGCAAAGT+AGG - chr1.2:22471896-22471915 None:intergenic 40.0%
TTCCTAATACTAGGCATAGG+TGG + chr1.2:22472262-22472281 MS.gene96444:CDS 40.0%
TTTCGGTTGTCGAAGTAAGT+TGG + chr1.2:22473426-22473445 MS.gene96444:intron 40.0%
! ACTCATTTTGCCCAACTTCT+TGG - chr1.2:22473210-22473229 None:intergenic 40.0%
! AGACATGCCCTTTTTGTACT+TGG + chr1.2:22472957-22472976 MS.gene96444:CDS 40.0%
! AGGAATACGAGGCAGATTTT+CGG + chr1.2:22472646-22472665 MS.gene96444:CDS 40.0%
! CATGGGCAATCCATTTTACT+AGG + chr1.2:22472099-22472118 MS.gene96444:CDS 40.0%
! CCCTTACGTGGTACTTTTAA+CGG - chr1.2:22473323-22473342 None:intergenic 40.0%
! GAATACCATTTTCGAAGAGC+GGG + chr1.2:22472458-22472477 MS.gene96444:CDS 40.0%
! GATTGCAAACCCTTGTTTCA+TGG - chr1.2:22471431-22471450 None:intergenic 40.0%
!! ATTCCTTTTCAGCCTTGTCT+TGG - chr1.2:22472632-22472651 None:intergenic 40.0%
!! CCGTTAAAAGTACCACGTAA+GGG + chr1.2:22473320-22473339 MS.gene96444:CDS 40.0%
!! TCCGTTAAAAGTACCACGTA+AGG + chr1.2:22473319-22473338 MS.gene96444:CDS 40.0%
!! TTAGAGGAGAGTATTGTCTG+TGG + chr1.2:22472837-22472856 MS.gene96444:CDS 40.0%
!!! CGGGTTGATGATTTTGTTGT+TGG + chr1.2:22472387-22472406 MS.gene96444:CDS 40.0%
ACTCCAAGACAAGGCTGAAA+AGG + chr1.2:22472626-22472645 MS.gene96444:CDS 45.0%
CAAGCACAATCATTCCATGC+GGG + chr1.2:22472673-22472692 MS.gene96444:CDS 45.0%
CAAGGCTGAAAAGGAATACG+AGG + chr1.2:22472635-22472654 MS.gene96444:CDS 45.0%
CACCACCTATGCCTAGTATT+AGG - chr1.2:22472267-22472286 None:intergenic 45.0%
CATCCTTTATTACCCCCTGA+CGG + chr1.2:22471256-22471275 MS.gene96444:CDS 45.0%
CTATGAGATTGACATGGACG+AGG + chr1.2:22471933-22471952 MS.gene96444:CDS 45.0%
GAACAACCAGGTAAAGGACT+TGG - chr1.2:22472151-22472170 None:intergenic 45.0%
GCTTGAAACCCATCAGACAT+AGG - chr1.2:22473563-22473582 None:intergenic 45.0%
GGATTCGTCTTCAAAGTTGG+TGG - chr1.2:22471544-22471563 None:intergenic 45.0%
GGCTATGCAGAATGCAATTC+AGG + chr1.2:22472233-22472252 MS.gene96444:CDS 45.0%
GGTTGTTCGAAACATGGCTA+AGG + chr1.2:22472163-22472182 MS.gene96444:CDS 45.0%
TAAACGATCAGGCAGGTGTA+CGG + chr1.2:22471731-22471750 MS.gene96444:CDS 45.0%
TAGCCATGTTTCGAACAACC+AGG - chr1.2:22472163-22472182 None:intergenic 45.0%
TCATCCCAACAGTCAACTAG+TGG + chr1.2:22473608-22473627 MS.gene96444:CDS 45.0%
TCGGTTACAATTCCCATTGG+AGG - chr1.2:22472204-22472223 None:intergenic 45.0%
TCTTTCCCGCTCTTCGAAAA+TGG - chr1.2:22472466-22472485 None:intergenic 45.0%
TTACAGCCACATTGTGGTGT+GGG + chr1.2:22473230-22473249 MS.gene96444:CDS 45.0%
TTTACAGCCACATTGTGGTG+TGG + chr1.2:22473229-22473248 MS.gene96444:CDS 45.0%
TTTCGAAGAGCGGGAAAGAT+GGG + chr1.2:22472467-22472486 MS.gene96444:CDS 45.0%
! CCTCTGTGTTGTTGACATTC+CGG - chr1.2:22472523-22472542 None:intergenic 45.0%
! TCTCGAAGGTGTGACTACAT+TGG + chr1.2:22472791-22472810 MS.gene96444:CDS 45.0%
! TTTTCGAAGAGCGGGAAAGA+TGG + chr1.2:22472466-22472485 MS.gene96444:CDS 45.0%
AAACGATCAGGCAGGTGTAC+GGG + chr1.2:22471732-22471751 MS.gene96444:CDS 50.0%
ACACCAACAAACGGGGCAAA+TGG - chr1.2:22472072-22472091 None:intergenic 50.0%
AGAAAGACACCTTCTCGGGA+AGG + chr1.2:22471846-22471865 MS.gene96444:CDS 50.0%
ATGTCTTCGAGGCCTCCAAT+GGG + chr1.2:22472189-22472208 MS.gene96444:CDS 50.0%
CCGGAATGTCAACAACACAG+AGG + chr1.2:22472520-22472539 MS.gene96444:CDS 50.0%
CGGCATCACATACCATGGAA+CGG - chr1.2:22471771-22471790 None:intergenic 50.0%
CGTTCCATGGTATGTGATGC+CGG + chr1.2:22471769-22471788 MS.gene96444:CDS 50.0%
CTAGGCATAGGTGGTGTCTA+TGG + chr1.2:22472271-22472290 MS.gene96444:CDS 50.0%
GACGTCCTCGAACTAATCGT+CGG + chr1.2:22473342-22473361 MS.gene96444:CDS 50.0%
GGCTAAGGTGTATGTCTTCG+AGG + chr1.2:22472178-22472197 MS.gene96444:CDS 50.0%
TATGTCTTCGAGGCCTCCAA+TGG + chr1.2:22472188-22472207 MS.gene96444:CDS 50.0%
TCTCCGTCAGGGGGTAATAA+AGG - chr1.2:22471262-22471281 None:intergenic 50.0%
! AACTCTAAGAAAGGGGCCGA+TGG + chr1.2:22471460-22471479 MS.gene96444:CDS 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG + chr1.2:22472066-22472085 MS.gene96444:CDS 50.0%
! CGTGTTGACGTTTCGGATTC+CGG + chr1.2:22471301-22471320 MS.gene96444:CDS 50.0%
! CTGCATAGCCTTGCACTGAT+CGG - chr1.2:22472223-22472242 None:intergenic 50.0%
! GACAACCGACGATTAGTTCG+AGG - chr1.2:22473350-22473369 None:intergenic 50.0%
! GTGTTGACGTTTCGGATTCC+GGG + chr1.2:22471302-22471321 MS.gene96444:CDS 50.0%
!! TCTGATGGGTTTCAAGCGTC+CGG + chr1.2:22473566-22473585 MS.gene96444:CDS 50.0%
ACCACCGGCATCACATACCA+TGG - chr1.2:22471776-22471795 None:intergenic 55.0%
AGACACCTTCTCGGGAAGGA+AGG + chr1.2:22471850-22471869 MS.gene96444:CDS 55.0%
AGCCACATTGTGGTGTGGGA+GGG + chr1.2:22473234-22473253 MS.gene96444:CDS 55.0%
CATCTCCTTCCTTCCCGAGA+AGG - chr1.2:22471858-22471877 None:intergenic 55.0%
CGACAACGCACTCCAAGACA+AGG + chr1.2:22472617-22472636 MS.gene96444:CDS 55.0%
CTTCTCGGGAAGGAAGGAGA+TGG + chr1.2:22471856-22471875 MS.gene96444:CDS 55.0%
TCCATGGTATGTGATGCCGG+TGG + chr1.2:22471772-22471791 MS.gene96444:CDS 55.0%
TCTGGGCTGATGCACGTAGT+AGG + chr1.2:22471977-22471996 MS.gene96444:CDS 55.0%
! ATCAGCTAGCTCTCCGTCAG+GGG - chr1.2:22471272-22471291 None:intergenic 55.0%
! CATCAGCTAGCTCTCCGTCA+GGG - chr1.2:22471273-22471292 None:intergenic 55.0%
! TGCGGCTCGTGTTGACGTTT+CGG + chr1.2:22471294-22471313 MS.gene96444:CDS 55.0%
CAGCCACATTGTGGTGTGGG+AGG + chr1.2:22473233-22473252 MS.gene96444:CDS 60.0%
CCTGACGGAGAGCTAGCTGA+TGG + chr1.2:22471271-22471290 MS.gene96444:CDS 60.0%
CGGGAAGGAAGGAGATGGCA+GGG + chr1.2:22471861-22471880 MS.gene96444:CDS 60.0%
GCCACATTGTGGTGTGGGAG+GGG + chr1.2:22473235-22473254 MS.gene96444:CDS 60.0%
GTTCGAGGACGTCCCTTACG+TGG - chr1.2:22473335-22473354 None:intergenic 60.0%
TCCCCTCCCACACCACAATG+TGG - chr1.2:22473239-22473258 None:intergenic 60.0%
TCGGGAAGGAAGGAGATGGC+AGG + chr1.2:22471860-22471879 MS.gene96444:CDS 60.0%
! CCATCAGCTAGCTCTCCGTC+AGG - chr1.2:22471274-22471293 None:intergenic 60.0%
! CGGAGAGCTAGCTGATGGTG+CGG + chr1.2:22471276-22471295 MS.gene96444:CDS 60.0%
! TCAGCTAGCTCTCCGTCAGG+GGG - chr1.2:22471271-22471290 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 22471226 22473685 22471226 ID=MS.gene96444
chr1.2 mRNA 22471226 22473685 22471226 ID=MS.gene96444.t1;Parent=MS.gene96444
chr1.2 exon 22471226 22473439 22471226 ID=MS.gene96444.t1.exon1;Parent=MS.gene96444.t1
chr1.2 CDS 22471226 22473439 22471226 ID=cds.MS.gene96444.t1;Parent=MS.gene96444.t1
chr1.2 exon 22473548 22473685 22473548 ID=MS.gene96444.t1.exon2;Parent=MS.gene96444.t1
chr1.2 CDS 22473548 22473685 22473548 ID=cds.MS.gene96444.t1;Parent=MS.gene96444.t1
Gene Sequence

>MS.gene96444

ATGGCTGATGGTCCTCCTATATTGATCGATCATCCTTTATTACCCCCTGACGGAGAGCTAGCTGATGGTGCGGCTCGTGTTGACGTTTCGGATTCCGGGACCTTTGAAACAACAAATGCTGTCGCAACAGATAAATTCACGCCTACTATCGGTATGTCTTTTAAATCGGTTGTAGATTGTAAGTCTTTTTACCGTGAATATGCCATGAAACAAGGGTTTGCAATCAAAACTAGAAACTCTAAGAAAGGGGCCGATGGCATGTTAAAATATTTCATTCTAACTTGTGCAAGAGAAGGTCACCATGAATCAAACATACCACCAACTTTGAAGACGAATCCAACGAAGAAACTTGATTGCTCAGCTAAGATAACGGTTGCTTTAAAGAAGGGTCTATGGACTATTTTGTCTTTTAATTCATGTCATAATCATGAGATAAGTCCAACTAAATCACGGTTGTTTGCTGGCAATAAGAAGATTGATATGCATGCACAGCGGACAATTTTGATAAACGATCAGGCAGGTGTACGGGTGAACAAAAGTTTCCGTTCCATGGTATGTGATGCCGGTGGTTACGAAAATCTAACATTTGTGGAGAGAGATGTCAGAAACTTTGTGGCAAAAGAAAGACACCTTCTCGGGAAGGAAGGAGATGGCAGGGCACTTTTATCCTACTTTGCTAGAATGAGGGAATGCAACAAAGACTTTTTCTATGAGATTGACATGGACGAGGACTTTCATGTGCAGAATATTTTCTGGGCTGATGCACGTAGTAGGTCTGCCTATGAATACTTTGGTGATGTTGTCACATTCGATACCACGTACTTGACAAACAAGTATGACATGCCATTTGCCCCGTTTGTTGGTGTCAATCATCATGGGCAATCCATTTTACTAGGTTGCGGTCTTTTATCATCTGAAGATACCAAGTCCTTTACCTGGTTGTTCGAAACATGGCTAAGGTGTATGTCTTCGAGGCCTCCAATGGGAATTGTAACCGATCAGTGCAAGGCTATGCAGAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGAAAAATTGCCGAAAAATTGAAAGGATACGCACAATACAAAGGAATAAAAAATGATATGAAAAATTGTGTGTATGATTCTTTGCGGGTTGATGATTTTGTTGTTGGGTGGACAACATTCATTGAAAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAAAATGATTTCTGGGCCGGAATGTCAACAACACAGAGGAGTGAAAGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTAAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCAGATTTTCGGTCTTCAAGCACAATCATTCCATGCGGGTCAAATTCAACAATCGAGAGACAATTTCAAGCCGAGTACACCCATGACAAGTATATGGAGATACAAGTAGAATTTCGTGGGAAATGCAACTGTTATCTCGAAGGTGTGACTACATTGGAATTTACATCTACATACACCGTTTTAGAGGAGAGTATTGTCTGTGGTAAACCGAACGAAACTAAGTATTGTGTTGATTTCAACGGGGAAGACAATAATGTTACGTGCACTTGTTTGTTGTTTGAGTTCAGAGGCATTTTGTGTAGACATGCCCTTTTTGTACTTGGTCATCTTAGAGTGAAAGAGGTTCCGTCTAAATTTATCTTGCCACGATGGAGTAAAAATGTGAAGAGAAAATATGCACACATGAAGACAAGTTACAATGCCAAAAAACTACAACCACACATTGAAAGATTTGAAGGTCTTTGCAAGACATTTTATGAAATTGCAGAATGTGCGGCTGAGACACCTAGTCAAACCTCTGATTTGTATGAAGAAATGATCAAATGGTCTACCAAGAAGTTGGGCAAAATGAGTTTACAGCCACATTGTGGTGTGGGAGGGGATCATAGCTTAAACACGATGGAGGACTCATTATCAATGCAACCTGATCAACCAATTCTTAATCCGTTAAAAGTACCACGTAAGGGACGTCCTCGAACTAATCGTCGGTTGTCCACCACTGAAAAGATTGTGAAAAAAAGAAGCAAAATGCAAAGAAAAAAACCAATTGTTTCGGTTGTCGAAGTAAGTTGGTGTTAACCTATTATTATTGTGATTACATTTGTTTTTTTAATAATAATACAATGTTAATTATGTTAAATGTAATTAATATTTCACTCTGTAATACTACAGCCTACATTACTTCCTATGTCTGATGGGTTTCAAGCGTCCGGTGATACGCTTAACTACTTCTCATCCCAACAGTCAACTAGTGGTTTTATGTCATTACTTGCATCAATGAGCAAAGATGTGGACGCAGCTGAATTTTAA
Protein sequence

>MS.gene96444.t1

MADGPPILIDHPLLPPDGELADGAARVDVSDSGTFETTNAVATDKFTPTIGMSFKSVVDCKSFYREYAMKQGFAIKTRNSKKGADGMLKYFILTCAREGHHESNIPPTLKTNPTKKLDCSAKITVALKKGLWTILSFNSCHNHEISPTKSRLFAGNKKIDMHAQRTILINDQAGVRVNKSFRSMVCDAGGYENLTFVERDVRNFVAKERHLLGKEGDGRALLSYFARMRECNKDFFYEIDMDEDFHVQNIFWADARSRSAYEYFGDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSILLGCGLLSSEDTKSFTWLFETWLRCMSSRPPMGIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKIAEKLKGYAQYKGIKNDMKNCVYDSLRVDDFVVGWTTFIEKYGLGENDWLNTIFEERERWVPCYLKNDFWAGMSTTQRSESMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPCGSNSTIERQFQAEYTHDKYMEIQVEFRGKCNCYLEGVTTLEFTSTYTVLEESIVCGKPNETKYCVDFNGEDNNVTCTCLLFEFRGILCRHALFVLGHLRVKEVPSKFILPRWSKNVKRKYAHMKTSYNAKKLQPHIERFEGLCKTFYEIAECAAETPSQTSDLYEEMIKWSTKKLGKMSLQPHCGVGGDHSLNTMEDSLSMQPDQPILNPLKVPRKGRPRTNRRLSTTEKIVKKRSKMQRKKPIVSVVEPTLLPMSDGFQASGDTLNYFSSQQSTSGFMSLLASMSKDVDAAEF