Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96702.t1 | XP_013469974.1 | 82.3 | 186 | 28 | 4 | 1 | 181 | 3 | 188 | 4.30E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96702.t1 | A0A072VQE3 | 82.3 | 186 | 28 | 4 | 1 | 181 | 3 | 188 | 3.1e-75 | 290.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene96702 | 0.804316 | 2.29E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene96702 | 0.803958 | 2.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96702.t1 | MTR_1g106080 | 93.011 | 186 | 8 | 4 | 1 | 181 | 3 | 188 | 1.79e-111 | 316 |
| MS.gene96702.t1 | MTR_5g005230 | 50.794 | 189 | 69 | 10 | 1 | 181 | 1 | 173 | 1.14e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96702.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 5.85e-21 | 85.1 |
| MS.gene96702.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 5.85e-21 | 85.1 |
Find 26 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.266605 | 1.1:-76339735 | MS.gene96702:CDS |
| TCATACCAGGAAGAACAATT+TGG | 0.315479 | 1.1:-76339220 | MS.gene96702:CDS |
| ACAAAGTTATGTAGGAAATA+TGG | 0.330154 | 1.1:-76339382 | MS.gene96702:CDS |
| TATGAAAACACTAGATTCAT+AGG | 0.343147 | 1.1:-76339840 | MS.gene96702:CDS |
| TATTTCCTACATAACTTTGT+TGG | 0.375448 | 1.1:+76339385 | None:intergenic |
| TGTAGTAGTGGATGTGAGTC+AGG | 0.376527 | 1.1:-76339888 | MS.gene96702:CDS |
| AGGAGTGGAAGATTTGTCTA+TGG | 0.397750 | 1.1:-76339754 | MS.gene96702:CDS |
| AAATTGTTCTTCCTGGTATG+AGG | 0.406008 | 1.1:+76339222 | None:intergenic |
| GGTGGAACTGAAGGGTACTA+TGG | 0.434994 | 1.1:-76339819 | MS.gene96702:CDS |
| GCAGTGTCATGAAGAAAAGA+AGG | 0.507146 | 1.1:+76339579 | None:intergenic |
| GGTACAAAGAAACAGAAAGT+TGG | 0.512053 | 1.1:-76339642 | MS.gene96702:CDS |
| ACAAACCAACAAAGTTATGT+AGG | 0.515352 | 1.1:-76339390 | MS.gene96702:CDS |
| TTGGTTTGTCCCAACAACAT+TGG | 0.519643 | 1.1:+76339404 | None:intergenic |
| ACATCATCATGTGGAAGATG+TGG | 0.558107 | 1.1:+76339708 | None:intergenic |
| AGATTCATAGGTGGAACTGA+AGG | 0.568350 | 1.1:-76339828 | MS.gene96702:CDS |
| GTCACAAATGAGTATTCTGA+AGG | 0.574749 | 1.1:-76339774 | MS.gene96702:CDS |
| AAATGAGTATTCTGAAGGAG+TGG | 0.595088 | 1.1:-76339769 | MS.gene96702:CDS |
| TTTGATTCTGAATGTAGTAG+TGG | 0.600879 | 1.1:-76339900 | MS.gene96702:CDS |
| GTAGGGAAAATCCTCATACC+AGG | 0.622430 | 1.1:-76339233 | MS.gene96702:intron |
| TCATCATGTGGAAGATGTGG+AGG | 0.636849 | 1.1:+76339711 | None:intergenic |
| TTGAAGTAAACATCATCATG+TGG | 0.637487 | 1.1:+76339699 | None:intergenic |
| GATTCATAGGTGGAACTGAA+GGG | 0.638366 | 1.1:-76339827 | MS.gene96702:CDS |
| GTAGTGGATGTGAGTCAGGT+TGG | 0.642732 | 1.1:-76339884 | MS.gene96702:CDS |
| TGTCATGAAGAAAAGAAGGA+AGG | 0.667075 | 1.1:+76339583 | None:intergenic |
| GAAAACACTAGATTCATAGG+TGG | 0.672436 | 1.1:-76339837 | MS.gene96702:CDS |
| AATATGGTAGACTATTCACA+AGG | 0.724521 | 1.1:-76339366 | MS.gene96702:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGTTTGTGTTATTTGT+AGG | - | chr1.1:76339833-76339852 | MS.gene96702:CDS | 20.0% |
| !!! | ACTAATTATTTTGAGGTAAT+TGG | - | chr1.1:76339751-76339770 | MS.gene96702:CDS | 20.0% |
| !!! | ATTTGTTTGTGTTATTTGTA+GGG | - | chr1.1:76339834-76339853 | MS.gene96702:CDS | 20.0% |
| ! | ACAAAGTTATGTAGGAAATA+TGG | - | chr1.1:76339702-76339721 | MS.gene96702:CDS | 25.0% |
| ! | TATGAAAACACTAGATTCAT+AGG | - | chr1.1:76339244-76339263 | MS.gene96702:intron | 25.0% |
| ! | TATTTCCTACATAACTTTGT+TGG | + | chr1.1:76339702-76339721 | None:intergenic | 25.0% |
| !! | CTTTTCAACTGTAAGAAATA+TGG | - | chr1.1:76339546-76339565 | MS.gene96702:intron | 25.0% |
| !! | TGTTTTCATAAGAATGTTCA+AGG | + | chr1.1:76339234-76339253 | None:intergenic | 25.0% |
| !!! | TTCAGCAACTAATTATTTTG+AGG | - | chr1.1:76339744-76339763 | MS.gene96702:CDS | 25.0% |
| AAAAACCAAATTGTTCTTCC+TGG | + | chr1.1:76339872-76339891 | None:intergenic | 30.0% | |
| AATATGGTAGACTATTCACA+AGG | - | chr1.1:76339718-76339737 | MS.gene96702:CDS | 30.0% | |
| ACAAACCAACAAAGTTATGT+AGG | - | chr1.1:76339694-76339713 | MS.gene96702:CDS | 30.0% | |
| CTCTCAAAAAATGAACTTCA+AGG | - | chr1.1:76339903-76339922 | MS.gene96702:CDS | 30.0% | |
| TTGAAGTAAACATCATCATG+TGG | + | chr1.1:76339388-76339407 | None:intergenic | 30.0% | |
| TTTGATTCTGAATGTAGTAG+TGG | - | chr1.1:76339184-76339203 | MS.gene96702:CDS | 30.0% | |
| !! | ATTTTTCAGACCAATGTTGT+TGG | - | chr1.1:76339670-76339689 | MS.gene96702:CDS | 30.0% |
| !! | TTTTTCAGACCAATGTTGTT+GGG | - | chr1.1:76339671-76339690 | MS.gene96702:CDS | 30.0% |
| !!! | AGTTCATTTTTTGAGAGAGA+TGG | + | chr1.1:76339900-76339919 | None:intergenic | 30.0% |
| AAATTGTTCTTCCTGGTATG+AGG | + | chr1.1:76339865-76339884 | None:intergenic | 35.0% | |
| GAAAACACTAGATTCATAGG+TGG | - | chr1.1:76339247-76339266 | MS.gene96702:intron | 35.0% | |
| GGTACAAAGAAACAGAAAGT+TGG | - | chr1.1:76339442-76339461 | MS.gene96702:intron | 35.0% | |
| GTCACAAATGAGTATTCTGA+AGG | - | chr1.1:76339310-76339329 | MS.gene96702:intron | 35.0% | |
| TCATACCAGGAAGAACAATT+TGG | - | chr1.1:76339864-76339883 | MS.gene96702:CDS | 35.0% | |
| TGTCATGAAGAAAAGAAGGA+AGG | + | chr1.1:76339504-76339523 | None:intergenic | 35.0% | |
| ! | AAATGAGTATTCTGAAGGAG+TGG | - | chr1.1:76339315-76339334 | MS.gene96702:intron | 35.0% |
| ! | GTAGTGAAAAAGCACAAGAA+AGG | - | chr1.1:76339421-76339440 | MS.gene96702:intron | 35.0% |
| ! | TGACTCTTTTGTCTTTGTGT+TGG | + | chr1.1:76339294-76339313 | None:intergenic | 35.0% |
| ACATCATCATGTGGAAGATG+TGG | + | chr1.1:76339379-76339398 | None:intergenic | 40.0% | |
| AGATTCATAGGTGGAACTGA+AGG | - | chr1.1:76339256-76339275 | MS.gene96702:intron | 40.0% | |
| AGGAGTGGAAGATTTGTCTA+TGG | - | chr1.1:76339330-76339349 | MS.gene96702:intron | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | - | chr1.1:76339349-76339368 | MS.gene96702:CDS | 40.0% | |
| GATTCATAGGTGGAACTGAA+GGG | - | chr1.1:76339257-76339276 | MS.gene96702:intron | 40.0% | |
| GCAGTGTCATGAAGAAAAGA+AGG | + | chr1.1:76339508-76339527 | None:intergenic | 40.0% | |
| !! | TTGGTTTGTCCCAACAACAT+TGG | + | chr1.1:76339683-76339702 | None:intergenic | 40.0% |
| GTAGGGAAAATCCTCATACC+AGG | - | chr1.1:76339851-76339870 | MS.gene96702:CDS | 45.0% | |
| TCATCATGTGGAAGATGTGG+AGG | + | chr1.1:76339376-76339395 | None:intergenic | 45.0% | |
| !! | TGTAGTAGTGGATGTGAGTC+AGG | - | chr1.1:76339196-76339215 | MS.gene96702:CDS | 45.0% |
| !! | GGTGGAACTGAAGGGTACTA+TGG | - | chr1.1:76339265-76339284 | MS.gene96702:intron | 50.0% |
| !! | GTAGTGGATGTGAGTCAGGT+TGG | - | chr1.1:76339200-76339219 | MS.gene96702:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76339175 | 76339931 | 76339175 | ID=MS.gene96702 |
| chr1.1 | mRNA | 76339175 | 76339931 | 76339175 | ID=MS.gene96702.t1;Parent=MS.gene96702 |
| chr1.1 | exon | 76339551 | 76339931 | 76339551 | ID=MS.gene96702.t1.exon1;Parent=MS.gene96702.t1 |
| chr1.1 | CDS | 76339551 | 76339931 | 76339551 | ID=cds.MS.gene96702.t1;Parent=MS.gene96702.t1 |
| chr1.1 | exon | 76339341 | 76339427 | 76339341 | ID=MS.gene96702.t1.exon2;Parent=MS.gene96702.t1 |
| chr1.1 | CDS | 76339341 | 76339427 | 76339341 | ID=cds.MS.gene96702.t1;Parent=MS.gene96702.t1 |
| chr1.1 | exon | 76339175 | 76339251 | 76339175 | ID=MS.gene96702.t1.exon3;Parent=MS.gene96702.t1 |
| chr1.1 | CDS | 76339175 | 76339251 | 76339175 | ID=cds.MS.gene96702.t1;Parent=MS.gene96702.t1 |
| Gene Sequence |
| Protein sequence |
