Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96703.t1 | XP_024631204.1 | 83.2 | 197 | 28 | 4 | 1 | 192 | 3 | 199 | 1.30E-82 | 315.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96703.t1 | A0A072VQE3 | 82.7 | 197 | 28 | 5 | 1 | 192 | 3 | 198 | 6.9e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene96703 | 0.815567 | 8.65E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene96703 | 0.800925 | 1.15E-48 | -1.69E-46 |
| MS.gene053581 | MS.gene96703 | 0.821791 | 3.35E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96703.t1 | MTR_1g106080 | 92.893 | 197 | 8 | 5 | 1 | 192 | 3 | 198 | 3.00e-118 | 333 |
| MS.gene96703.t1 | MTR_5g005230 | 51.020 | 196 | 72 | 10 | 1 | 188 | 1 | 180 | 5.96e-44 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96703.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.16e-20 | 84.7 |
| MS.gene96703.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.16e-20 | 84.7 |
Find 28 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.266605 | 1.1:+76207354 | MS.gene96703:CDS |
| TCATACCAGGAAGAACAATT+TGG | 0.315479 | 1.1:+76207869 | MS.gene96703:CDS |
| ACAAAGTTATGTAGGAAATA+TGG | 0.330154 | 1.1:+76207707 | MS.gene96703:CDS |
| TATGAAAACACTAGATTCAT+AGG | 0.343147 | 1.1:+76207249 | MS.gene96703:CDS |
| TATTTCCTACATAACTTTGT+TGG | 0.375448 | 1.1:-76207704 | None:intergenic |
| TGTAGTAGTGGATGTGAGTC+AGG | 0.376527 | 1.1:+76207201 | MS.gene96703:CDS |
| AGGAGTGGAAGATTTGTCTA+TGG | 0.397750 | 1.1:+76207335 | MS.gene96703:CDS |
| AAATTGTTCTTCCTGGTATG+AGG | 0.406008 | 1.1:-76207867 | None:intergenic |
| TCTTTGTTTAGCAGCTGGTA+TGG | 0.422441 | 1.1:+76208024 | MS.gene96703:intron |
| GGTGGAACTGAAGGGTACTA+TGG | 0.434994 | 1.1:+76207270 | MS.gene96703:CDS |
| GCAGTGTCATGAAGAAAAGA+AGG | 0.507146 | 1.1:-76207510 | None:intergenic |
| GGTACAAAGAAACAGAAAGT+TGG | 0.512053 | 1.1:+76207447 | MS.gene96703:CDS |
| ACAAACCAACAAAGTTATGT+AGG | 0.515352 | 1.1:+76207699 | MS.gene96703:CDS |
| TTGGTTTGTCCCAACAACAT+TGG | 0.519643 | 1.1:-76207685 | None:intergenic |
| ACATCATCATGTGGAAGATG+TGG | 0.558107 | 1.1:-76207381 | None:intergenic |
| AGATTCATAGGTGGAACTGA+AGG | 0.568350 | 1.1:+76207261 | MS.gene96703:CDS |
| GTCACAAATGAGTATTCTGA+AGG | 0.574749 | 1.1:+76207315 | MS.gene96703:CDS |
| AAATGAGTATTCTGAAGGAG+TGG | 0.595088 | 1.1:+76207320 | MS.gene96703:CDS |
| TTGTTTAGCAGCTGGTATGG+AGG | 0.596105 | 1.1:+76208027 | MS.gene96703:intron |
| TTTGATTCTGAATGTAGTAG+TGG | 0.600879 | 1.1:+76207189 | MS.gene96703:CDS |
| GTAGGGAAAATCCTCATACC+AGG | 0.622430 | 1.1:+76207856 | MS.gene96703:intron |
| TCATCATGTGGAAGATGTGG+AGG | 0.636849 | 1.1:-76207378 | None:intergenic |
| TTGAAGTAAACATCATCATG+TGG | 0.637487 | 1.1:-76207390 | None:intergenic |
| GATTCATAGGTGGAACTGAA+GGG | 0.638366 | 1.1:+76207262 | MS.gene96703:CDS |
| GTAGTGGATGTGAGTCAGGT+TGG | 0.642732 | 1.1:+76207205 | MS.gene96703:CDS |
| TGTCATGAAGAAAAGAAGGA+AGG | 0.667075 | 1.1:-76207506 | None:intergenic |
| GAAAACACTAGATTCATAGG+TGG | 0.672436 | 1.1:+76207252 | MS.gene96703:CDS |
| AATATGGTAGACTATTCACA+AGG | 0.724521 | 1.1:+76207723 | MS.gene96703:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGTTTGTGTTATTTGT+AGG | + | chr1.1:76207838-76207857 | MS.gene96703:intron | 20.0% |
| !!! | ACTAATTATTTTGAGGTAAT+TGG | + | chr1.1:76207756-76207775 | MS.gene96703:intron | 20.0% |
| !!! | ATTTGTTTGTGTTATTTGTA+GGG | + | chr1.1:76207839-76207858 | MS.gene96703:intron | 20.0% |
| ! | ACAAAGTTATGTAGGAAATA+TGG | + | chr1.1:76207707-76207726 | MS.gene96703:CDS | 25.0% |
| ! | TATGAAAACACTAGATTCAT+AGG | + | chr1.1:76207249-76207268 | MS.gene96703:CDS | 25.0% |
| ! | TATTTCCTACATAACTTTGT+TGG | - | chr1.1:76207707-76207726 | None:intergenic | 25.0% |
| !! | CTTTTCAACTGTAAGAAATA+TGG | + | chr1.1:76207551-76207570 | MS.gene96703:intron | 25.0% |
| !! | TGTTTTCATAAGAATGTTCA+AGG | - | chr1.1:76207239-76207258 | None:intergenic | 25.0% |
| !!! | TTCAGCAACTAATTATTTTG+AGG | + | chr1.1:76207749-76207768 | MS.gene96703:CDS | 25.0% |
| AAAAACCAAATTGTTCTTCC+TGG | - | chr1.1:76207877-76207896 | None:intergenic | 30.0% | |
| AATATGGTAGACTATTCACA+AGG | + | chr1.1:76207723-76207742 | MS.gene96703:CDS | 30.0% | |
| ACAAACCAACAAAGTTATGT+AGG | + | chr1.1:76207699-76207718 | MS.gene96703:CDS | 30.0% | |
| CTCTCAAAAAATGAACTTCA+AGG | + | chr1.1:76207908-76207927 | MS.gene96703:CDS | 30.0% | |
| TTGAAGTAAACATCATCATG+TGG | - | chr1.1:76207393-76207412 | None:intergenic | 30.0% | |
| TTTGATTCTGAATGTAGTAG+TGG | + | chr1.1:76207189-76207208 | MS.gene96703:CDS | 30.0% | |
| !! | ATTTTTCAGACCAATGTTGT+TGG | + | chr1.1:76207675-76207694 | MS.gene96703:intron | 30.0% |
| !! | TTTTTCAGACCAATGTTGTT+GGG | + | chr1.1:76207676-76207695 | MS.gene96703:intron | 30.0% |
| !!! | AGTTCATTTTTTGAGAGAGA+TGG | - | chr1.1:76207905-76207924 | None:intergenic | 30.0% |
| AAATTGTTCTTCCTGGTATG+AGG | - | chr1.1:76207870-76207889 | None:intergenic | 35.0% | |
| GAAAACACTAGATTCATAGG+TGG | + | chr1.1:76207252-76207271 | MS.gene96703:CDS | 35.0% | |
| GGTACAAAGAAACAGAAAGT+TGG | + | chr1.1:76207447-76207466 | MS.gene96703:CDS | 35.0% | |
| GTCACAAATGAGTATTCTGA+AGG | + | chr1.1:76207315-76207334 | MS.gene96703:CDS | 35.0% | |
| TCATACCAGGAAGAACAATT+TGG | + | chr1.1:76207869-76207888 | MS.gene96703:CDS | 35.0% | |
| TGTCATGAAGAAAAGAAGGA+AGG | - | chr1.1:76207509-76207528 | None:intergenic | 35.0% | |
| ! | AAATGAGTATTCTGAAGGAG+TGG | + | chr1.1:76207320-76207339 | MS.gene96703:CDS | 35.0% |
| ! | GTAGTGAAAAAGCACAAGAA+AGG | + | chr1.1:76207426-76207445 | MS.gene96703:CDS | 35.0% |
| ! | TGACTCTTTTGTCTTTGTGT+TGG | - | chr1.1:76207299-76207318 | None:intergenic | 35.0% |
| ! | TTCATTCTTTGTTTAGCAGC+TGG | + | chr1.1:76208019-76208038 | MS.gene96703:intron | 35.0% |
| ACATCATCATGTGGAAGATG+TGG | - | chr1.1:76207384-76207403 | None:intergenic | 40.0% | |
| AGATTCATAGGTGGAACTGA+AGG | + | chr1.1:76207261-76207280 | MS.gene96703:CDS | 40.0% | |
| AGGAGTGGAAGATTTGTCTA+TGG | + | chr1.1:76207335-76207354 | MS.gene96703:CDS | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | + | chr1.1:76207354-76207373 | MS.gene96703:CDS | 40.0% | |
| GATTCATAGGTGGAACTGAA+GGG | + | chr1.1:76207262-76207281 | MS.gene96703:CDS | 40.0% | |
| GCAGTGTCATGAAGAAAAGA+AGG | - | chr1.1:76207513-76207532 | None:intergenic | 40.0% | |
| ! | TCTTTGTTTAGCAGCTGGTA+TGG | + | chr1.1:76208024-76208043 | MS.gene96703:intron | 40.0% |
| !! | TTGGTTTGTCCCAACAACAT+TGG | - | chr1.1:76207688-76207707 | None:intergenic | 40.0% |
| GTAGGGAAAATCCTCATACC+AGG | + | chr1.1:76207856-76207875 | MS.gene96703:intron | 45.0% | |
| TCATCATGTGGAAGATGTGG+AGG | - | chr1.1:76207381-76207400 | None:intergenic | 45.0% | |
| TTGTTTAGCAGCTGGTATGG+AGG | + | chr1.1:76208027-76208046 | MS.gene96703:intron | 45.0% | |
| !! | TGTAGTAGTGGATGTGAGTC+AGG | + | chr1.1:76207201-76207220 | MS.gene96703:CDS | 45.0% |
| !! | GGTGGAACTGAAGGGTACTA+TGG | + | chr1.1:76207270-76207289 | MS.gene96703:CDS | 50.0% |
| !! | GTAGTGGATGTGAGTCAGGT+TGG | + | chr1.1:76207205-76207224 | MS.gene96703:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76207180 | 76208068 | 76207180 | ID=MS.gene96703 |
| chr1.1 | mRNA | 76207180 | 76208068 | 76207180 | ID=MS.gene96703.t1;Parent=MS.gene96703 |
| chr1.1 | exon | 76207180 | 76207560 | 76207180 | ID=MS.gene96703.t1.exon1;Parent=MS.gene96703.t1 |
| chr1.1 | CDS | 76207180 | 76207560 | 76207180 | ID=cds.MS.gene96703.t1;Parent=MS.gene96703.t1 |
| chr1.1 | exon | 76207684 | 76207770 | 76207684 | ID=MS.gene96703.t1.exon2;Parent=MS.gene96703.t1 |
| chr1.1 | CDS | 76207684 | 76207770 | 76207684 | ID=cds.MS.gene96703.t1;Parent=MS.gene96703.t1 |
| chr1.1 | exon | 76207860 | 76207936 | 76207860 | ID=MS.gene96703.t1.exon3;Parent=MS.gene96703.t1 |
| chr1.1 | CDS | 76207860 | 76207936 | 76207860 | ID=cds.MS.gene96703.t1;Parent=MS.gene96703.t1 |
| chr1.1 | exon | 76208035 | 76208068 | 76208035 | ID=MS.gene96703.t1.exon4;Parent=MS.gene96703.t1 |
| chr1.1 | CDS | 76208035 | 76208068 | 76208035 | ID=cds.MS.gene96703.t1;Parent=MS.gene96703.t1 |
| Gene Sequence |
| Protein sequence |
