Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98047.t1 | KEH28209.1 | 96.3 | 164 | 6 | 0 | 5 | 168 | 373 | 536 | 3.50E-79 | 304.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98047.t1 | Q682S0 | 60.9 | 179 | 55 | 3 | 5 | 168 | 415 | 593 | 7.2e-49 | 194.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98047.t1 | A0A072UEL2 | 96.3 | 164 | 6 | 0 | 5 | 168 | 373 | 536 | 2.5e-79 | 304.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene98047 | 0.805185 | 1.51E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene98047 | 0.824853 | 6.44E-54 | -1.69E-46 |
| MS.gene053559 | MS.gene98047 | 0.80617 | 9.37E-50 | -1.69E-46 |
| MS.gene060495 | MS.gene98047 | 0.824201 | 9.18E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98047.t1 | MTR_5g075640 | 96.341 | 164 | 6 | 0 | 5 | 168 | 373 | 536 | 8.16e-111 | 325 |
| MS.gene98047.t1 | MTR_5g075640 | 96.341 | 164 | 6 | 0 | 5 | 168 | 416 | 579 | 3.39e-110 | 325 |
| MS.gene98047.t1 | MTR_3g062540 | 71.598 | 169 | 37 | 4 | 5 | 168 | 364 | 526 | 1.07e-73 | 230 |
| MS.gene98047.t1 | MTR_3g062540 | 71.598 | 169 | 37 | 4 | 5 | 168 | 406 | 568 | 3.29e-73 | 229 |
| MS.gene98047.t1 | MTR_5g016060 | 35.119 | 168 | 77 | 3 | 5 | 140 | 325 | 492 | 1.39e-24 | 99.4 |
| MS.gene98047.t1 | MTR_5g016060 | 35.119 | 168 | 77 | 3 | 5 | 140 | 439 | 606 | 1.90e-24 | 99.0 |
| MS.gene98047.t1 | MTR_4g095430 | 32.275 | 189 | 99 | 5 | 5 | 168 | 411 | 595 | 8.55e-23 | 94.4 |
| MS.gene98047.t1 | MTR_1g088885 | 31.472 | 197 | 87 | 6 | 5 | 168 | 440 | 621 | 3.25e-19 | 84.0 |
| MS.gene98047.t1 | MTR_4g022330 | 32.026 | 153 | 83 | 2 | 5 | 140 | 437 | 585 | 1.03e-17 | 79.7 |
| MS.gene98047.t1 | MTR_7g073980 | 32.450 | 151 | 87 | 3 | 5 | 142 | 435 | 583 | 1.30e-17 | 79.3 |
| MS.gene98047.t1 | MTR_7g108290 | 30.864 | 162 | 73 | 3 | 5 | 138 | 444 | 594 | 1.18e-14 | 70.9 |
| MS.gene98047.t1 | MTR_2g084595 | 31.405 | 121 | 63 | 2 | 5 | 105 | 442 | 562 | 9.46e-14 | 68.6 |
| MS.gene98047.t1 | MTR_1g094850 | 37.209 | 86 | 50 | 2 | 5 | 86 | 440 | 525 | 2.21e-12 | 64.3 |
| MS.gene98047.t1 | MTR_8g078900 | 30.500 | 200 | 90 | 9 | 5 | 163 | 430 | 621 | 3.99e-12 | 63.5 |
| MS.gene98047.t1 | MTR_8g078900 | 30.500 | 200 | 90 | 9 | 5 | 163 | 420 | 611 | 4.09e-12 | 63.5 |
| MS.gene98047.t1 | MTR_8g078900 | 30.500 | 200 | 90 | 9 | 5 | 163 | 460 | 651 | 4.38e-12 | 63.5 |
| MS.gene98047.t1 | MTR_2g018750 | 41.379 | 58 | 34 | 0 | 5 | 62 | 444 | 501 | 1.96e-11 | 61.6 |
| MS.gene98047.t1 | MTR_2g048375 | 32.540 | 126 | 70 | 4 | 25 | 135 | 1 | 126 | 2.19e-11 | 59.7 |
| MS.gene98047.t1 | MTR_2g009340 | 43.860 | 57 | 32 | 0 | 7 | 63 | 496 | 552 | 8.83e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98047.t1 | AT2G30520 | 65.363 | 179 | 47 | 4 | 5 | 168 | 367 | 545 | 6.55e-69 | 218 |
| MS.gene98047.t1 | AT2G30520 | 65.363 | 179 | 47 | 4 | 5 | 168 | 367 | 545 | 6.55e-69 | 218 |
| MS.gene98047.t1 | AT2G30520 | 65.363 | 179 | 47 | 4 | 5 | 168 | 415 | 593 | 1.96e-68 | 218 |
| MS.gene98047.t1 | AT5G67385 | 35.385 | 195 | 85 | 5 | 5 | 168 | 420 | 604 | 2.17e-25 | 101 |
| MS.gene98047.t1 | AT5G67385 | 35.385 | 195 | 85 | 5 | 5 | 168 | 375 | 559 | 2.47e-25 | 101 |
| MS.gene98047.t1 | AT5G67385 | 35.385 | 195 | 85 | 5 | 5 | 168 | 375 | 559 | 2.47e-25 | 101 |
| MS.gene98047.t1 | AT5G67385 | 35.385 | 195 | 85 | 5 | 5 | 168 | 375 | 559 | 2.47e-25 | 101 |
| MS.gene98047.t1 | AT5G67385 | 35.385 | 195 | 85 | 5 | 5 | 168 | 375 | 559 | 2.47e-25 | 101 |
| MS.gene98047.t1 | AT1G03010 | 30.952 | 168 | 67 | 4 | 5 | 138 | 422 | 574 | 4.84e-17 | 77.8 |
| MS.gene98047.t1 | AT1G03010 | 30.952 | 168 | 67 | 4 | 5 | 138 | 449 | 601 | 5.28e-17 | 77.8 |
| MS.gene98047.t1 | AT1G03010 | 30.952 | 168 | 67 | 4 | 5 | 138 | 448 | 600 | 6.27e-17 | 77.4 |
| MS.gene98047.t1 | AT3G08660 | 35.099 | 151 | 74 | 4 | 7 | 140 | 354 | 497 | 8.53e-17 | 77.0 |
| MS.gene98047.t1 | AT3G08660 | 35.099 | 151 | 74 | 4 | 7 | 140 | 414 | 557 | 9.68e-17 | 77.0 |
| MS.gene98047.t1 | AT1G30440 | 29.064 | 203 | 96 | 4 | 5 | 164 | 454 | 651 | 5.01e-16 | 75.1 |
| MS.gene98047.t1 | AT5G48800 | 32.317 | 164 | 72 | 4 | 5 | 140 | 430 | 582 | 1.72e-15 | 73.6 |
| MS.gene98047.t1 | AT2G47860 | 31.707 | 123 | 63 | 1 | 5 | 106 | 463 | 585 | 8.87e-15 | 71.2 |
| MS.gene98047.t1 | AT2G47860 | 31.707 | 123 | 63 | 1 | 5 | 106 | 440 | 562 | 8.89e-15 | 71.2 |
| MS.gene98047.t1 | AT2G47860 | 31.707 | 123 | 63 | 1 | 5 | 106 | 322 | 444 | 1.19e-14 | 70.9 |
| MS.gene98047.t1 | AT2G47860 | 31.707 | 123 | 63 | 1 | 5 | 106 | 322 | 444 | 1.19e-14 | 70.9 |
| MS.gene98047.t1 | AT3G08570 | 28.994 | 169 | 79 | 4 | 5 | 146 | 436 | 590 | 3.60e-14 | 69.7 |
| MS.gene98047.t1 | AT3G50840 | 38.318 | 107 | 59 | 2 | 5 | 105 | 405 | 510 | 1.93e-13 | 67.4 |
| MS.gene98047.t1 | AT3G50840 | 38.318 | 107 | 59 | 2 | 5 | 105 | 369 | 474 | 2.24e-13 | 67.4 |
| MS.gene98047.t1 | AT3G50840 | 38.318 | 107 | 59 | 2 | 5 | 105 | 369 | 474 | 2.24e-13 | 67.4 |
| MS.gene98047.t1 | AT5G66560 | 47.368 | 57 | 30 | 0 | 5 | 61 | 476 | 532 | 1.09e-12 | 65.5 |
| MS.gene98047.t1 | AT5G66560 | 47.368 | 57 | 30 | 0 | 5 | 61 | 437 | 493 | 1.25e-12 | 65.1 |
| MS.gene98047.t1 | AT5G66560 | 47.368 | 57 | 30 | 0 | 5 | 61 | 447 | 503 | 1.32e-12 | 65.1 |
| MS.gene98047.t1 | AT3G44820 | 30.508 | 118 | 65 | 2 | 5 | 105 | 455 | 572 | 1.44e-12 | 65.1 |
| MS.gene98047.t1 | AT3G49970 | 30.899 | 178 | 92 | 6 | 5 | 168 | 355 | 515 | 2.80e-12 | 63.9 |
| MS.gene98047.t1 | AT3G49970 | 30.899 | 178 | 92 | 6 | 5 | 168 | 355 | 515 | 2.80e-12 | 63.9 |
| MS.gene98047.t1 | AT5G03250 | 37.705 | 61 | 38 | 0 | 5 | 65 | 448 | 508 | 3.73e-11 | 60.8 |
| MS.gene98047.t1 | AT5G13600 | 26.389 | 144 | 104 | 1 | 5 | 146 | 439 | 582 | 3.80e-11 | 60.8 |
| MS.gene98047.t1 | AT5G03250 | 37.705 | 61 | 38 | 0 | 5 | 65 | 336 | 396 | 3.83e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
| MS.gene98047.t1 | AT1G67900 | 35.526 | 76 | 47 | 1 | 5 | 80 | 455 | 528 | 4.08e-11 | 60.8 |
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAGAACCTTCTCCTTCT+AGG | 0.245811 | 5.1:+58163827 | None:intergenic |
| TCGTCCGTGTCAAAGAAATT+AGG | 0.274325 | 5.1:-58163923 | MS.gene98047:CDS |
| AGTTGAGAACGGAGTTGATT+AGG | 0.340136 | 5.1:-58164042 | MS.gene98047:CDS |
| AAACAAAATAATCTATGAAA+TGG | 0.401564 | 5.1:+58163808 | None:intergenic |
| CTCTTCCGCGTCACGTTCCA+TGG | 0.404029 | 5.1:+58164111 | None:intergenic |
| GTCTCAGAACAAACGATTAC+CGG | 0.410250 | 5.1:-58164191 | MS.gene98047:CDS |
| TGCACTTAAACTTTCATATG+AGG | 0.412052 | 5.1:-58164227 | MS.gene98047:CDS |
| TGGATTTGACAAAGCCTAGA+AGG | 0.433974 | 5.1:-58163841 | MS.gene98047:CDS |
| CTTCTCTAACTCATCAAGAT+TGG | 0.455111 | 5.1:+58164273 | None:intergenic |
| GTGGGCATGTGTGATGTTGC+AGG | 0.456100 | 5.1:-58164302 | MS.gene98047:CDS |
| ATCAATTAAAGATAAGAAGC+GGG | 0.464447 | 5.1:-58164138 | MS.gene98047:CDS |
| GGCGGAACAACTTCATCGTC+TGG | 0.474475 | 5.1:-58163980 | MS.gene98047:CDS |
| GTTACAAACAGACGTAACTT+TGG | 0.492774 | 5.1:-58164080 | MS.gene98047:CDS |
| ACAATGCATGTAGTACAATC+TGG | 0.498695 | 5.1:+58164167 | None:intergenic |
| GGAGAAAGTGTGCAGTGTGA+TGG | 0.507183 | 5.1:-58164251 | MS.gene98047:CDS |
| ACAACTTCATCGTCTGGAGT+TGG | 0.509583 | 5.1:-58163974 | MS.gene98047:CDS |
| TAAAGATACGTCTCATATTG+AGG | 0.512243 | 5.1:-58163873 | MS.gene98047:CDS |
| GATAAGAAGCGGGGCAACCA+TGG | 0.513146 | 5.1:-58164128 | MS.gene98047:CDS |
| GTTTCAGATTTGCAAAAGAA+CGG | 0.543872 | 5.1:-58164007 | MS.gene98047:CDS |
| AAGCTGAATCCGTTCAAGAA+TGG | 0.550799 | 5.1:-58163899 | MS.gene98047:CDS |
| TCTTTAGAACCATTCTTGAA+CGG | 0.565852 | 5.1:+58163890 | None:intergenic |
| TGACAAAGCCTAGAAGGAGA+AGG | 0.583766 | 5.1:-58163835 | MS.gene98047:CDS |
| CTTACCTAATTTCTTTGACA+CGG | 0.586200 | 5.1:+58163919 | None:intergenic |
| ACGTCTCATATTGAGGATGT+TGG | 0.590809 | 5.1:-58163866 | MS.gene98047:CDS |
| GCATGTAGTACAATCTGGAC+CGG | 0.592690 | 5.1:+58164172 | None:intergenic |
| TGGAACGTGACGCGGAAGAG+AGG | 0.597151 | 5.1:-58164108 | MS.gene98047:CDS |
| GGCAACCATGGAACGTGACG+CGG | 0.599493 | 5.1:-58164116 | MS.gene98047:CDS |
| GATCAATTAAAGATAAGAAG+CGG | 0.602164 | 5.1:-58164139 | MS.gene98047:CDS |
| GATTTGCAAAAGAACGGTCA+CGG | 0.603295 | 5.1:-58164001 | MS.gene98047:CDS |
| AGAGAACGAGGAGTTGAGAA+CGG | 0.616083 | 5.1:-58164053 | MS.gene98047:CDS |
| CAATCTTGATGAGTTAGAGA+AGG | 0.623085 | 5.1:-58164272 | MS.gene98047:CDS |
| TCATATTGAGGATGTTGGTG+TGG | 0.644673 | 5.1:-58163861 | MS.gene98047:CDS |
| AACTTTGGTGAGAGAGAACG+AGG | 0.672959 | 5.1:-58164065 | MS.gene98047:CDS |
| TGACACGGACGAAAAGAATG+TGG | 0.683709 | 5.1:+58163934 | None:intergenic |
| TCAATTAAAGATAAGAAGCG+GGG | 0.687813 | 5.1:-58164137 | MS.gene98047:CDS |
| TTGCAAAAGAACGGTCACGG+CGG | 0.698644 | 5.1:-58163998 | MS.gene98047:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCAATTAAAGATAAGAAGC+GGG | - | chr5.1:58163986-58164005 | MS.gene98047:CDS | 25.0% |
| ! | GATCAATTAAAGATAAGAAG+CGG | - | chr5.1:58163985-58164004 | MS.gene98047:CDS | 25.0% |
| CTTACCTAATTTCTTTGACA+CGG | + | chr5.1:58164208-58164227 | None:intergenic | 30.0% | |
| GTTTCAGATTTGCAAAAGAA+CGG | - | chr5.1:58164117-58164136 | MS.gene98047:CDS | 30.0% | |
| TAAAGATACGTCTCATATTG+AGG | - | chr5.1:58164251-58164270 | MS.gene98047:CDS | 30.0% | |
| TCAATTAAAGATAAGAAGCG+GGG | - | chr5.1:58163987-58164006 | MS.gene98047:CDS | 30.0% | |
| TGCACTTAAACTTTCATATG+AGG | - | chr5.1:58163897-58163916 | MS.gene98047:CDS | 30.0% | |
| !! | TCTTTAGAACCATTCTTGAA+CGG | + | chr5.1:58164237-58164256 | None:intergenic | 30.0% |
| ACAATGCATGTAGTACAATC+TGG | + | chr5.1:58163960-58163979 | None:intergenic | 35.0% | |
| CAATCTTGATGAGTTAGAGA+AGG | - | chr5.1:58163852-58163871 | MS.gene98047:CDS | 35.0% | |
| CTTCTCTAACTCATCAAGAT+TGG | + | chr5.1:58163854-58163873 | None:intergenic | 35.0% | |
| GTTACAAACAGACGTAACTT+TGG | - | chr5.1:58164044-58164063 | MS.gene98047:CDS | 35.0% | |
| AAGCTGAATCCGTTCAAGAA+TGG | - | chr5.1:58164225-58164244 | MS.gene98047:CDS | 40.0% | |
| ACGTCTCATATTGAGGATGT+TGG | - | chr5.1:58164258-58164277 | MS.gene98047:CDS | 40.0% | |
| GATTTGCAAAAGAACGGTCA+CGG | - | chr5.1:58164123-58164142 | MS.gene98047:CDS | 40.0% | |
| GTCTCAGAACAAACGATTAC+CGG | - | chr5.1:58163933-58163952 | MS.gene98047:CDS | 40.0% | |
| TCATATTGAGGATGTTGGTG+TGG | - | chr5.1:58164263-58164282 | MS.gene98047:CDS | 40.0% | |
| TCGTCCGTGTCAAAGAAATT+AGG | - | chr5.1:58164201-58164220 | MS.gene98047:CDS | 40.0% | |
| TGGATTTGACAAAGCCTAGA+AGG | - | chr5.1:58164283-58164302 | MS.gene98047:CDS | 40.0% | |
| ! | AGTTGAGAACGGAGTTGATT+AGG | - | chr5.1:58164082-58164101 | MS.gene98047:CDS | 40.0% |
| AACTTTGGTGAGAGAGAACG+AGG | - | chr5.1:58164059-58164078 | MS.gene98047:CDS | 45.0% | |
| AGAGAACGAGGAGTTGAGAA+CGG | - | chr5.1:58164071-58164090 | MS.gene98047:CDS | 45.0% | |
| ATGGAGAACCTTCTCCTTCT+AGG | + | chr5.1:58164300-58164319 | None:intergenic | 45.0% | |
| GCATGTAGTACAATCTGGAC+CGG | + | chr5.1:58163955-58163974 | None:intergenic | 45.0% | |
| TGACAAAGCCTAGAAGGAGA+AGG | - | chr5.1:58164289-58164308 | MS.gene98047:CDS | 45.0% | |
| TGACACGGACGAAAAGAATG+TGG | + | chr5.1:58164193-58164212 | None:intergenic | 45.0% | |
| ! | ACAACTTCATCGTCTGGAGT+TGG | - | chr5.1:58164150-58164169 | MS.gene98047:CDS | 45.0% |
| TTGCAAAAGAACGGTCACGG+CGG | - | chr5.1:58164126-58164145 | MS.gene98047:CDS | 50.0% | |
| ! | GGAGAAAGTGTGCAGTGTGA+TGG | - | chr5.1:58163873-58163892 | MS.gene98047:CDS | 50.0% |
| GATAAGAAGCGGGGCAACCA+TGG | - | chr5.1:58163996-58164015 | MS.gene98047:CDS | 55.0% | |
| GGCGGAACAACTTCATCGTC+TGG | - | chr5.1:58164144-58164163 | MS.gene98047:CDS | 55.0% | |
| GTGGGCATGTGTGATGTTGC+AGG | - | chr5.1:58163822-58163841 | MS.gene98047:CDS | 55.0% | |
| CTCTTCCGCGTCACGTTCCA+TGG | + | chr5.1:58164016-58164035 | None:intergenic | 60.0% | |
| GGCAACCATGGAACGTGACG+CGG | - | chr5.1:58164008-58164027 | MS.gene98047:CDS | 60.0% | |
| TGGAACGTGACGCGGAAGAG+AGG | - | chr5.1:58164016-58164035 | MS.gene98047:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 58163820 | 58164326 | 58163820 | ID=MS.gene98047 |
| chr5.1 | mRNA | 58163820 | 58164326 | 58163820 | ID=MS.gene98047.t1;Parent=MS.gene98047 |
| chr5.1 | exon | 58163820 | 58164326 | 58163820 | ID=MS.gene98047.t1.exon1;Parent=MS.gene98047.t1 |
| chr5.1 | CDS | 58163820 | 58164326 | 58163820 | ID=cds.MS.gene98047.t1;Parent=MS.gene98047.t1 |
| Gene Sequence |
| Protein sequence |
