Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98201.t1 | XP_013444753.1 | 84 | 737 | 101 | 3 | 1 | 729 | 1 | 728 | 6.50E-279 | 969.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98201.t1 | Q84WU4 | 44.7 | 722 | 327 | 7 | 1 | 663 | 1 | 709 | 1.8e-104 | 381.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98201.t1 | A0A072TPK8 | 84.0 | 737 | 101 | 3 | 1 | 729 | 1 | 728 | 4.7e-279 | 969.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene98201 | 0.806006 | 1.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98201.t1 | MTR_8g028445 | 90.773 | 737 | 51 | 3 | 1 | 729 | 1 | 728 | 0.0 | 1301 |
| MS.gene98201.t1 | MTR_8g028445 | 86.567 | 737 | 49 | 4 | 1 | 729 | 1 | 695 | 0.0 | 1224 |
| MS.gene98201.t1 | MTR_7g079200 | 59.949 | 789 | 222 | 11 | 1 | 729 | 1 | 755 | 0.0 | 823 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98201.t1 | AT3G61570 | 50.956 | 732 | 269 | 8 | 1 | 664 | 1 | 710 | 0.0 | 612 |
| MS.gene98201.t1 | AT2G46180 | 51.183 | 676 | 270 | 10 | 1 | 624 | 1 | 668 | 0.0 | 587 |
| MS.gene98201.t1 | AT3G61570 | 52.648 | 661 | 251 | 6 | 1 | 612 | 1 | 648 | 0.0 | 583 |
Find 116 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATATCAATAACTATTTA+AGG | 0.109699 | 8.2:-68440838 | MS.gene98201:CDS |
| CCGCCTGGTGGGAGGCATTT+TGG | 0.120326 | 8.2:-68439101 | MS.gene98201:CDS |
| CTTTGATTTGTTGAATTTAT+TGG | 0.132770 | 8.2:+68438462 | None:intergenic |
| CTTCAGCGTCCTGAAAATTT+TGG | 0.182636 | 8.2:-68438414 | MS.gene98201:CDS |
| CGTTCCATCTCTTCCTTTCC+AGG | 0.245138 | 8.2:+68441984 | None:intergenic |
| TGCTTTGCATGTTCAGAAAA+AGG | 0.246253 | 8.2:-68442164 | MS.gene98201:CDS |
| GTTAGATTAAATAAAGAAAA+TGG | 0.247810 | 8.2:-68443997 | MS.gene98201:CDS |
| AACTTCAACTTTCTTATCTC+TGG | 0.256550 | 8.2:+68443548 | None:intergenic |
| AAAGCTAAGTAACGATTTAA+AGG | 0.274490 | 8.2:-68441952 | MS.gene98201:CDS |
| TGGTTCAGTAAAGCAGAATT+TGG | 0.295545 | 8.2:-68443977 | MS.gene98201:CDS |
| TGCTCTAGAACCTTCAATTC+TGG | 0.320172 | 8.2:+68443927 | None:intergenic |
| CCATCTGATGCCAAAACTTC+TGG | 0.327140 | 8.2:-68438363 | MS.gene98201:CDS |
| TTCTTGAATGTCAGAGGAAC+AGG | 0.329926 | 8.2:-68443398 | MS.gene98201:intron |
| CTGGTTTGAACCAGAAGTTT+TGG | 0.339738 | 8.2:+68438353 | None:intergenic |
| CAGTTTGTGCCTTCTCAGAT+TGG | 0.339758 | 8.2:+68440075 | None:intergenic |
| CCGCCTTAAGCAGCATTTGC+TGG | 0.345493 | 8.2:-68441889 | MS.gene98201:CDS |
| CGGCGAATCCGTTAGAATTC+CGG | 0.367770 | 8.2:+68447152 | None:intergenic |
| TCACTTCCCAATCAGAGGTT+TGG | 0.369228 | 8.2:-68438534 | MS.gene98201:CDS |
| CGGCTGCCTGGCCGCCTGGT+GGG | 0.376481 | 8.2:-68439112 | MS.gene98201:CDS |
| AGGAATTGAACAAATCTTTC+CGG | 0.396110 | 8.2:-68443378 | MS.gene98201:CDS |
| TGATATCAATAACTATTTAA+GGG | 0.399881 | 8.2:-68440837 | MS.gene98201:CDS |
| TTTCCGGCTGCCTGGCCGCC+TGG | 0.405050 | 8.2:-68439116 | MS.gene98201:CDS |
| CTCAACAGCACGCCTCAATC+TGG | 0.407754 | 8.2:+68440013 | None:intergenic |
| GGAATTGAACAAATCTTTCC+GGG | 0.408237 | 8.2:-68443377 | MS.gene98201:CDS |
| TAAGCAGCATTTGCTGGAAC+AGG | 0.408433 | 8.2:-68441883 | MS.gene98201:intron |
| CCACCAGGCGGCCAGGCAGC+CGG | 0.425887 | 8.2:+68439113 | None:intergenic |
| GAATGAAAATACCACTCTTA+AGG | 0.428058 | 8.2:-68442399 | MS.gene98201:intron |
| CCTCTCTGCTGTGATTTCTC+TGG | 0.428853 | 8.2:+68439639 | None:intergenic |
| GGAGTAGTGGAAATAGTATC+TGG | 0.429531 | 8.2:+68438558 | None:intergenic |
| ACTTCAACTTTCTTATCTCT+GGG | 0.431630 | 8.2:+68443549 | None:intergenic |
| TCAAATTTACAGACAGCTCT+TGG | 0.434287 | 8.2:-68440647 | MS.gene98201:CDS |
| ATGCCTCCCACCAGGCGGCC+AGG | 0.434666 | 8.2:+68439106 | None:intergenic |
| TTGAGCGCCTCCAGAATTGA+AGG | 0.434999 | 8.2:-68443937 | MS.gene98201:CDS |
| GCAGATGCAGACCTCTGATT+AGG | 0.438854 | 8.2:+68438489 | None:intergenic |
| TAAAGGAAACGCGGCGGGAC+AGG | 0.444379 | 8.2:-68441935 | MS.gene98201:CDS |
| GTCCGAAAATGATGAAACTT+TGG | 0.445193 | 8.2:-68440108 | MS.gene98201:CDS |
| GAGGGAGAAGAGTGAAATTG+AGG | 0.447456 | 8.2:-68442236 | MS.gene98201:CDS |
| CACAATTCCTGATTTGAATC+CGG | 0.447532 | 8.2:+68447132 | None:intergenic |
| CTTCTCAGATTGGGAAAGCT+TGG | 0.448234 | 8.2:+68440085 | None:intergenic |
| AGAGAATCAGCAGGAAATCC+AGG | 0.449152 | 8.2:-68438612 | MS.gene98201:CDS |
| CCGGCTGCCTGGCCGCCTGG+TGG | 0.450487 | 8.2:-68439113 | MS.gene98201:CDS |
| AGTTTGTGCCTTCTCAGATT+GGG | 0.451143 | 8.2:+68440076 | None:intergenic |
| AACAGAGCCAAACCTCTGAT+TGG | 0.457171 | 8.2:+68438527 | None:intergenic |
| TTGGCATCAGATGGTGTAAG+AGG | 0.461334 | 8.2:+68438372 | None:intergenic |
| GGATTCGCCGGATTCAAATC+AGG | 0.468193 | 8.2:-68447139 | MS.gene98201:CDS |
| ATTGTTCAGCTAAAATTTCG+TGG | 0.471503 | 8.2:-68443494 | MS.gene98201:intron |
| CTGAGAAGGCACAAACTGAA+TGG | 0.475453 | 8.2:-68440070 | MS.gene98201:CDS |
| CTGCCTGGCCGCCTGGTGGG+AGG | 0.477369 | 8.2:-68439109 | MS.gene98201:CDS |
| GATCTAATGGTTCGCATGCT+AGG | 0.477870 | 8.2:-68439211 | MS.gene98201:CDS |
| CTCCCAAAATGCCTCCCACC+AGG | 0.481315 | 8.2:+68439098 | None:intergenic |
| ACAGAGCCAAACCTCTGATT+GGG | 0.490372 | 8.2:+68438528 | None:intergenic |
| AGAATTCCGGCGATCCGATA+CGG | 0.492583 | 8.2:+68447165 | None:intergenic |
| TGATTAGGAAGTGGTGGAAG+TGG | 0.493799 | 8.2:+68438504 | None:intergenic |
| ATTCAGATTATCTCGTTGAC+AGG | 0.494051 | 8.2:-68439956 | MS.gene98201:intron |
| GTTCCAGCAAATGCTGCTTA+AGG | 0.501645 | 8.2:+68441886 | None:intergenic |
| TTAACTTGTTAAGCTCCTCC+CGG | 0.510061 | 8.2:+68443359 | None:intergenic |
| CGATTTAAAGGAAACGCGGC+GGG | 0.510981 | 8.2:-68441940 | MS.gene98201:CDS |
| ACTGAAGCAGCGGCAAATGC+AGG | 0.516474 | 8.2:-68439067 | MS.gene98201:CDS |
| CAGGTCATCAATGATTTCCC+TGG | 0.519514 | 8.2:+68440724 | None:intergenic |
| CTATGCTGAAATTGAAGCCA+AGG | 0.526393 | 8.2:-68440618 | MS.gene98201:intron |
| AGAGAAAAGAGAGAATCAGC+AGG | 0.527435 | 8.2:-68438621 | MS.gene98201:CDS |
| CTTCTCTTTCAAATTCGCAA+TGG | 0.528960 | 8.2:+68447231 | None:intergenic |
| ATTGGGAAGTGAGGGAGTAG+TGG | 0.532142 | 8.2:+68438545 | None:intergenic |
| TCAGGACGCTGAAGGTAGCT+AGG | 0.537989 | 8.2:+68438423 | None:intergenic |
| TTCTTTAATTTCTGCCTCAG+AGG | 0.538016 | 8.2:+68444580 | None:intergenic |
| GAGCTTGCAGATTCAGCTGA+TGG | 0.541813 | 8.2:-68443605 | MS.gene98201:CDS |
| GATGAAGATAAGCAAAGGAT+AGG | 0.545733 | 8.2:-68439181 | MS.gene98201:CDS |
| ATTGTGTCGCCACTTGCTAA+TGG | 0.546195 | 8.2:-68447115 | MS.gene98201:CDS |
| ATATTGAAACTCTAAAGTCC+AGG | 0.549175 | 8.2:-68440742 | MS.gene98201:CDS |
| CGGAATTCTAACGGATTCGC+CGG | 0.552806 | 8.2:-68447151 | MS.gene98201:CDS |
| TCCTTAGGAACATTTAGAAG+AGG | 0.562466 | 8.2:-68440441 | MS.gene98201:intron |
| AAGTCACGAGGAACTGAAGA+TGG | 0.564366 | 8.2:-68440771 | MS.gene98201:CDS |
| ATTTAGAAGAGGAGTTAGCC+TGG | 0.565395 | 8.2:-68440430 | MS.gene98201:CDS |
| AACCTCTGATTGGGAAGTGA+GGG | 0.566359 | 8.2:+68438537 | None:intergenic |
| CAAGCTTTCCCAATCTGAGA+AGG | 0.570967 | 8.2:-68440084 | MS.gene98201:CDS |
| AGGAAGACAATGCCAGATTG+AGG | 0.571117 | 8.2:-68440025 | MS.gene98201:CDS |
| TTCAGTAAAGCAGAATTTGG+AGG | 0.571293 | 8.2:-68443974 | MS.gene98201:CDS |
| AGATAGAGTAATCAAGCTTG+AGG | 0.575976 | 8.2:-68440045 | MS.gene98201:CDS |
| GCAAGCAACGGTGTTATTCG+AGG | 0.576267 | 8.2:-68439142 | MS.gene98201:CDS |
| GACCTCTGATTAGGAAGTGG+TGG | 0.577542 | 8.2:+68438498 | None:intergenic |
| CTCCCTCACTTCCCAATCAG+AGG | 0.579124 | 8.2:-68438539 | MS.gene98201:CDS |
| GCAGACCTCTGATTAGGAAG+TGG | 0.579807 | 8.2:+68438495 | None:intergenic |
| TTTAGAAGAGGAGTTAGCCT+GGG | 0.586913 | 8.2:-68440429 | MS.gene98201:CDS |
| CGAGAGACTTCAAGCCTCTG+AGG | 0.592592 | 8.2:-68444594 | MS.gene98201:CDS |
| TGCAATTTGAATTGACTAGA+CGG | 0.593014 | 8.2:-68442481 | MS.gene98201:CDS |
| AATCTTCCGTATCGGATCGC+CGG | 0.598955 | 8.2:-68447171 | MS.gene98201:CDS |
| AAACCTCTGATTGGGAAGTG+AGG | 0.600364 | 8.2:+68438536 | None:intergenic |
| AAAGGAAACGCGGCGGGACA+GGG | 0.600503 | 8.2:-68441934 | MS.gene98201:CDS |
| AGGATAGGCATTGCTCAGCA+AGG | 0.608375 | 8.2:-68439166 | MS.gene98201:CDS |
| TCGGATCGCCGGAATTCTAA+CGG | 0.608847 | 8.2:-68447160 | MS.gene98201:CDS |
| TATTGAAACTCTAAAGTCCA+GGG | 0.613706 | 8.2:-68440741 | MS.gene98201:CDS |
| AGAAGATGAATCTTCCGTAT+CGG | 0.616171 | 8.2:-68447179 | MS.gene98201:CDS |
| CGATCAACACCATTAGCAAG+TGG | 0.618211 | 8.2:+68447106 | None:intergenic |
| GCTCAGCAAGGTGCAAGCAA+CGG | 0.625316 | 8.2:-68439154 | MS.gene98201:CDS |
| ACGGTTAAATAGAATGTCAG+TGG | 0.625896 | 8.2:-68439979 | MS.gene98201:CDS |
| CTTCAACTTTCTTATCTCTG+GGG | 0.628412 | 8.2:+68443550 | None:intergenic |
| GGACGCTGAAGGTAGCTAGG+AGG | 0.629960 | 8.2:+68438426 | None:intergenic |
| TTCCACCACTTCCTAATCAG+AGG | 0.636229 | 8.2:-68438500 | MS.gene98201:CDS |
| AAATGCAGGAACTGATAACC+AGG | 0.636294 | 8.2:-68439053 | MS.gene98201:intron |
| GGTTCTAGAGCATCTACAAG+TGG | 0.639219 | 8.2:-68443916 | MS.gene98201:CDS |
| AGGAAGTTCGACTGAAGCAG+CGG | 0.640792 | 8.2:-68439077 | MS.gene98201:CDS |
| AAATCTACCAACCTTAAGAG+TGG | 0.646425 | 8.2:+68442388 | None:intergenic |
| TCTCTGATGAAGATAAGCAA+AGG | 0.651782 | 8.2:-68439186 | MS.gene98201:CDS |
| CCAGCAAATGCTGCTTAAGG+CGG | 0.653754 | 8.2:+68441889 | None:intergenic |
| GCAAGCAATTGCAAGTCACG+AGG | 0.659317 | 8.2:-68440783 | MS.gene98201:CDS |
| TCTAGAACCTTCAATTCTGG+AGG | 0.665371 | 8.2:+68443930 | None:intergenic |
| TGATCGTGTTTCGTTCCCTG+AGG | 0.666646 | 8.2:-68447089 | MS.gene98201:intron |
| CCAAAATGCCTCCCACCAGG+CGG | 0.682218 | 8.2:+68439101 | None:intergenic |
| CTGTTGAGCAGAGTATGACA+CGG | 0.697732 | 8.2:-68439998 | MS.gene98201:CDS |
| CCAGAGAAATCACAGCAGAG+AGG | 0.697834 | 8.2:-68439639 | MS.gene98201:intron |
| GCAAAACGAAGAATCAGCAG+AGG | 0.698306 | 8.2:-68442459 | MS.gene98201:CDS |
| AGCTAGGAGGAAGAAGACTG+AGG | 0.700740 | 8.2:+68438439 | None:intergenic |
| ATTGAACAAATCTTTCCGGG+AGG | 0.719578 | 8.2:-68443374 | MS.gene98201:CDS |
| ATAGATAGAACAGTACAAAG+CGG | 0.728142 | 8.2:-68444621 | MS.gene98201:intron |
| GTAACGATTTAAAGGAAACG+CGG | 0.734663 | 8.2:-68441944 | MS.gene98201:CDS |
| ACGATTTAAAGGAAACGCGG+CGG | 0.739125 | 8.2:-68441941 | MS.gene98201:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 68438338 | 68447263 | 68438338 | ID=MS.gene98201 |
| chr8.2 | mRNA | 68438338 | 68447263 | 68438338 | ID=MS.gene98201.t1;Parent=MS.gene98201 |
| chr8.2 | exon | 68447090 | 68447263 | 68447090 | ID=MS.gene98201.t1.exon1;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68447090 | 68447263 | 68447090 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68444541 | 68444639 | 68444541 | ID=MS.gene98201.t1.exon2;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68444541 | 68444639 | 68444541 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68443900 | 68444028 | 68443900 | ID=MS.gene98201.t1.exon3;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68443900 | 68444028 | 68443900 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68443495 | 68443627 | 68443495 | ID=MS.gene98201.t1.exon4;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68443495 | 68443627 | 68443495 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68443342 | 68443406 | 68443342 | ID=MS.gene98201.t1.exon5;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68443342 | 68443406 | 68443342 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68442400 | 68442567 | 68442400 | ID=MS.gene98201.t1.exon6;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68442400 | 68442567 | 68442400 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68442144 | 68442257 | 68442144 | ID=MS.gene98201.t1.exon7;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68442144 | 68442257 | 68442144 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68441884 | 68442030 | 68441884 | ID=MS.gene98201.t1.exon8;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68441884 | 68442030 | 68441884 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68440619 | 68440915 | 68440619 | ID=MS.gene98201.t1.exon9;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68440619 | 68440915 | 68440619 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68440391 | 68440456 | 68440391 | ID=MS.gene98201.t1.exon10;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68440391 | 68440456 | 68440391 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68439957 | 68440159 | 68439957 | ID=MS.gene98201.t1.exon11;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68439957 | 68440159 | 68439957 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68439640 | 68439694 | 68439640 | ID=MS.gene98201.t1.exon12;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68439640 | 68439694 | 68439640 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68439054 | 68439239 | 68439054 | ID=MS.gene98201.t1.exon13;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68439054 | 68439239 | 68439054 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| chr8.2 | exon | 68438338 | 68438691 | 68438338 | ID=MS.gene98201.t1.exon14;Parent=MS.gene98201.t1 |
| chr8.2 | CDS | 68438338 | 68438691 | 68438338 | ID=cds.MS.gene98201.t1;Parent=MS.gene98201.t1 |
| Gene Sequence |
| Protein sequence |
