AlfalfaGEDB | MS.gene98734

M. sativa cultivar XinJiangDaYe / MS.gene98734

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052056	MS.gene98734	-0.801235	9.91E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98734.t1	MTR_4g080720	92.308	26	2	0	1	26	1	26	3.93e-11	53.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 30 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGCGACCGCCCGGGGTCGC+CGG	0.267437	2.1:+5386285	None:intergenic
GGCGACCCCGGGCGGTCGCC+CGG	0.272076	2.1:-5386283	MS.gene98734:CDS
TCGAGCCCGGGCGACCGCCC+GGG	0.322736	2.1:+5386277	None:intergenic
ATCGAGCCCGGGCGACCGCC+CGG	0.328256	2.1:+5386276	None:intergenic
GGCGACCGCCCGGGGTCGCC+GGG	0.329843	2.1:+5386286	None:intergenic
TGTTCAGTGTAGAAAGTATC+TGG	0.334155	2.1:+5386428	None:intergenic
TACTTTACCTGATTCTGTTC+CGG	0.342394	2.1:-5386102	MS.gene98734:CDS
TTGTTGACCGGAACAGAATC+AGG	0.356106	2.1:+5386095	None:intergenic
GCGACCCCGGGCGGTCGCCC+GGG	0.390046	2.1:-5386282	MS.gene98734:CDS
GAGACAGAGCCCGGCGACCC+CGG	0.397050	2.1:-5386295	MS.gene98734:CDS
GGTTCTATGTCTAGAAGAAT+TGG	0.399284	2.1:-5386146	MS.gene98734:CDS
GTTTCAGAAAGTGGGTTGTC+AGG	0.415088	2.1:-5385975	MS.gene98734:CDS
GGCGGTCGCCCGGGCTCGAT+CGG	0.426836	2.1:-5386273	MS.gene98734:intron
AGACAGAGCCCGGCGACCCC+GGG	0.435092	2.1:-5386294	MS.gene98734:CDS
AAAATGAAGTTCATGAAACT+TGG	0.444516	2.1:-5386458	MS.gene98734:CDS
AGGAACTTACCGATCGAGCC+CGG	0.460044	2.1:+5386264	None:intergenic
ATTTGATCAGTTTCAGAAAG+TGG	0.462774	2.1:-5385984	MS.gene98734:intron
CGAGCCCGGGCGACCGCCCG+GGG	0.485962	2.1:+5386278	None:intergenic
GCAGTGGCGGAGACAGAGCC+CGG	0.507968	2.1:-5386304	MS.gene98734:CDS
AGCAGAACCTCTTTGTTGAC+CGG	0.514149	2.1:+5386083	None:intergenic
TTTGATCAGTTTCAGAAAGT+GGG	0.517044	2.1:-5385983	MS.gene98734:intron
TTCTGTTCCGGTCAACAAAG+AGG	0.555294	2.1:-5386090	MS.gene98734:intron
CAACATTCTCAGAGGTTCGA+AGG	0.558149	2.1:-5386167	MS.gene98734:intron
AAATGAAGTTCATGAAACTT+GGG	0.559279	2.1:-5386457	MS.gene98734:CDS
CAGAGCCCGGCGACCCCGGG+CGG	0.568493	2.1:-5386291	MS.gene98734:CDS
GACATTGACATCAGCAGTGG+CGG	0.569095	2.1:-5386317	MS.gene98734:CDS
GGAACTTACCGATCGAGCCC+GGG	0.581615	2.1:+5386265	None:intergenic
TCTACACTGAACAAGCTACC+AGG	0.607293	2.1:-5386418	MS.gene98734:intron
AAAGTGGGTTGTCAGGATTG+TGG	0.612526	2.1:-5385968	MS.gene98734:CDS
CAGGACATTGACATCAGCAG+TGG	0.657018	2.1:-5386320	MS.gene98734:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTCAATTTCTTTTTT+GGG	-	chr2.1:5386360-5386379	MS.gene98734:intron	10.0%
!!!	TTTTTTTTCAATTTCTTTTT+TGG	-	chr2.1:5386359-5386378	MS.gene98734:intron	10.0%
!!	ACTTCAAAAACAAAAAAATT+GGG	-	chr2.1:5386197-5386216	MS.gene98734:intron	15.0%
!!	CACTTCAAAAACAAAAAAAT+TGG	-	chr2.1:5386196-5386215	MS.gene98734:intron	20.0%
!	AACAAAAAAATTGGGCAAAT+TGG	-	chr2.1:5386205-5386224	MS.gene98734:intron	25.0%
!	ACAAAAAAATTGGGCAAATT+GGG	-	chr2.1:5386206-5386225	MS.gene98734:intron	25.0%
!!!	AATACATGTTAGTTTGTGAA+AGG	+	chr2.1:5386065-5386084	None:intergenic	25.0%
!!!	TTGTTTTTGAAGTGTGTAAA+TGG	+	chr2.1:5386190-5386209	None:intergenic	25.0%
	ACATGTATTCTCATAAATGC+AGG	-	chr2.1:5386076-5386095	MS.gene98734:intron	30.0%
	ATTTGATCAGTTTCAGAAAG+TGG	-	chr2.1:5386431-5386450	MS.gene98734:CDS	30.0%
	TTTGATCAGTTTCAGAAAGT+GGG	-	chr2.1:5386432-5386451	MS.gene98734:CDS	30.0%
!!!	TTTTGAAGTGTGTAAATGGA+GGG	+	chr2.1:5386186-5386205	None:intergenic	30.0%
!!!	TTTTTGAAGTGTGTAAATGG+AGG	+	chr2.1:5386187-5386206	None:intergenic	30.0%
	GGTTCTATGTCTAGAAGAAT+TGG	-	chr2.1:5386269-5386288	MS.gene98734:intron	35.0%
	TACTTTACCTGATTCTGTTC+CGG	-	chr2.1:5386313-5386332	MS.gene98734:CDS	35.0%
!!	TGTTCAGTGTAGAAAGTATC+TGG	+	chr2.1:5385990-5386009	None:intergenic	35.0%
!!!	TTTTGGGTTTGCATGCTTTT+TGG	-	chr2.1:5386376-5386395	MS.gene98734:intron	35.0%
	CACTTCAACAACATTCTCAG+AGG	-	chr2.1:5386240-5386259	MS.gene98734:intron	40.0%
!	TAAATGGAGGGTGTAATGTG+AGG	+	chr2.1:5386174-5386193	None:intergenic	40.0%
	AGCAGAACCTCTTTGTTGAC+CGG	+	chr2.1:5386335-5386354	None:intergenic	45.0%
	CAACATTCTCAGAGGTTCGA+AGG	-	chr2.1:5386248-5386267	MS.gene98734:intron	45.0%
	GGGCAAATTGGGCAAATCTA+TGG	-	chr2.1:5386217-5386236	MS.gene98734:intron	45.0%
	TCTACACTGAACAAGCTACC+AGG	-	chr2.1:5385997-5386016	MS.gene98734:intron	45.0%
	TTCTGTTCCGGTCAACAAAG+AGG	-	chr2.1:5386325-5386344	MS.gene98734:intron	45.0%
	TTGTTGACCGGAACAGAATC+AGG	+	chr2.1:5386323-5386342	None:intergenic	45.0%
!!	AAAGTGGGTTGTCAGGATTG+TGG	-	chr2.1:5386447-5386466	MS.gene98734:CDS	45.0%
!!	GTTTCAGAAAGTGGGTTGTC+AGG	-	chr2.1:5386440-5386459	MS.gene98734:CDS	45.0%
	CAAACGCTGCTGTGAATACC+TGG	+	chr2.1:5386018-5386037	None:intergenic	50.0%
	CAGGACATTGACATCAGCAG+TGG	-	chr2.1:5386095-5386114	MS.gene98734:CDS	50.0%
	GACATTGACATCAGCAGTGG+CGG	-	chr2.1:5386098-5386117	MS.gene98734:CDS	50.0%
	AGGAACTTACCGATCGAGCC+CGG	+	chr2.1:5386154-5386173	None:intergenic	55.0%
	GGAACTTACCGATCGAGCCC+GGG	+	chr2.1:5386153-5386172	None:intergenic	60.0%
!	GCAGTGGCGGAGACAGAGCC+CGG	-	chr2.1:5386111-5386130	MS.gene98734:CDS	70.0%
	AGACAGAGCCCGGCGACCCC+GGG	-	chr2.1:5386121-5386140	MS.gene98734:CDS	75.0%
	GAGACAGAGCCCGGCGACCC+CGG	-	chr2.1:5386120-5386139	MS.gene98734:CDS	75.0%
!	ATCGAGCCCGGGCGACCGCC+CGG	+	chr2.1:5386142-5386161	None:intergenic	80.0%
!	GGCGGTCGCCCGGGCTCGAT+CGG	-	chr2.1:5386142-5386161	MS.gene98734:CDS	80.0%
!	CAGAGCCCGGCGACCCCGGG+CGG	-	chr2.1:5386124-5386143	MS.gene98734:CDS	85.0%
!	TCGAGCCCGGGCGACCGCCC+GGG	+	chr2.1:5386141-5386160	None:intergenic	85.0%
!	CGAGCCCGGGCGACCGCCCG+GGG	+	chr2.1:5386140-5386159	None:intergenic	90.0%
!	GCGACCCCGGGCGGTCGCCC+GGG	-	chr2.1:5386133-5386152	MS.gene98734:CDS	90.0%
!	GGCGACCCCGGGCGGTCGCC+CGG	-	chr2.1:5386132-5386151	MS.gene98734:CDS	90.0%
!	GGCGACCGCCCGGGGTCGCC+GGG	+	chr2.1:5386132-5386151	None:intergenic	90.0%
!	GGGCGACCGCCCGGGGTCGC+CGG	+	chr2.1:5386133-5386152	None:intergenic	90.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	5385960	5386477	5385960	ID=MS.gene98734
chr2.1	mRNA	5385960	5386477	5385960	ID=MS.gene98734.t1;Parent=MS.gene98734
chr2.1	exon	5386419	5386477	5386419	ID=MS.gene98734.t1.exon1;Parent=MS.gene98734.t1
chr2.1	CDS	5386419	5386477	5386419	ID=cds.MS.gene98734.t1;Parent=MS.gene98734.t1
chr2.1	exon	5386274	5386339	5386274	ID=MS.gene98734.t1.exon2;Parent=MS.gene98734.t1
chr2.1	CDS	5386274	5386339	5386274	ID=cds.MS.gene98734.t1;Parent=MS.gene98734.t1
chr2.1	exon	5386091	5386177	5386091	ID=MS.gene98734.t1.exon3;Parent=MS.gene98734.t1
chr2.1	CDS	5386091	5386177	5386091	ID=cds.MS.gene98734.t1;Parent=MS.gene98734.t1
chr2.1	exon	5385960	5385996	5385960	ID=MS.gene98734.t1.exon4;Parent=MS.gene98734.t1
chr2.1	CDS	5385960	5385996	5385960	ID=cds.MS.gene98734.t1;Parent=MS.gene98734.t1

Gene Sequence

>MS.gene98734

ATGAAGTTCATGAAACTTGGGACAAAGCCAGATACTTTCTACACTGAACAAGCTACCAGGTATTCACAGCAGCGTTTGTTTCATTAACAATCACTGTACAATCCTTTCACAAACTAACATGTATTCTCATAAATGCAGGACATTGACATCAGCAGTGGCGGAGACAGAGCCCGGCGACCCCGGGCGGTCGCCCGGGCTCGATCGGTAAGTTCCTCACATTACACCCTCCATTTACACACTTCAAAAACAAAAAAATTGGGCAAATTGGGCAAATCTATGGCACTTCAACAACATTCTCAGAGGTTCGAAGGTTCTATGTCTAGAAGAATTGGTTCTACTAGCTCTAGAAGACTTACTTTACCTGATTCTGTTCCGGTCAACAAAGAGGTTCTGCTCTTTTTTTTTTTCAATTTCTTTTTTGGGTTTGCATGCTTTTTGGATTATTGATTGTAAAGTTTTGATTTTTGAATCATTTGATCAGTTTCAGAAAGTGGGTTGTCAGGATTGTGGTTTATTGA

Protein sequence

>MS.gene98734.t1

MKFMKLGTKPDTFYTEQATRTLTSAVAETEPGDPGRSPGLDRGSKVLCLEELVLLALEDLLYLILFRSTKSFRKWVVRIVVY