Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98841.t1 | XP_003620485.1 | 98.8 | 167 | 2 | 0 | 1 | 167 | 1 | 167 | 2.10E-92 | 348.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98841.t1 | G7KN44 | 98.8 | 167 | 2 | 0 | 1 | 167 | 1 | 167 | 1.5e-92 | 348.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05460 | MS.gene98841 | 0.884209 | 2.33E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98841.t1 | MTR_6g084470 | 98.802 | 167 | 2 | 0 | 1 | 167 | 1 | 167 | 8.77e-122 | 340 |
| MS.gene98841.t1 | MTR_8g104210 | 64.368 | 174 | 48 | 7 | 3 | 167 | 4 | 172 | 8.26e-61 | 186 |
| MS.gene98841.t1 | MTR_8g104210 | 66.049 | 162 | 42 | 6 | 14 | 167 | 7 | 163 | 9.16e-59 | 181 |
| MS.gene98841.t1 | MTR_6g018430 | 42.857 | 175 | 85 | 6 | 1 | 167 | 1 | 168 | 7.64e-30 | 107 |
| MS.gene98841.t1 | MTR_1g110760 | 44.099 | 161 | 71 | 5 | 21 | 167 | 5 | 160 | 5.47e-22 | 87.0 |
| MS.gene98841.t1 | MTR_4g087400 | 37.888 | 161 | 66 | 7 | 1 | 153 | 1 | 135 | 9.23e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98841.t1 | AT3G02555 | 46.893 | 177 | 69 | 6 | 1 | 167 | 1 | 162 | 1.28e-32 | 114 |
| MS.gene98841.t1 | AT3G02555 | 46.893 | 177 | 69 | 6 | 1 | 167 | 1 | 162 | 1.28e-32 | 114 |
| MS.gene98841.t1 | AT5G16110 | 46.739 | 184 | 75 | 8 | 1 | 167 | 67 | 244 | 2.77e-32 | 115 |
| MS.gene98841.t1 | AT1G68490 | 40.341 | 176 | 86 | 5 | 5 | 167 | 14 | 183 | 7.77e-27 | 100 |
| MS.gene98841.t1 | AT1G13390 | 38.462 | 182 | 90 | 9 | 1 | 167 | 2 | 176 | 4.59e-20 | 82.4 |
| MS.gene98841.t1 | AT1G13390 | 38.462 | 182 | 90 | 9 | 1 | 167 | 2 | 176 | 4.59e-20 | 82.4 |
Find 48 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCTATAGTGAAAGATTT+GGG | 0.272791 | 6.1:+73995458 | MS.gene98841:CDS |
| AGACCAAATCGAATAGGTTT+AGG | 0.280991 | 6.1:-73994793 | None:intergenic |
| GAAAGATTTGGGGAGCAATT+AGG | 0.318109 | 6.1:+73995469 | MS.gene98841:CDS |
| CAGTCAGAGGTTGAAGATTC+AGG | 0.321864 | 6.1:+73994978 | MS.gene98841:CDS |
| CTCAAAATTTGTGGCCTAAA+GGG | 0.326387 | 6.1:-73994838 | None:intergenic |
| GTGATCCAAGATGAACAATT+TGG | 0.329036 | 6.1:+73995547 | MS.gene98841:CDS |
| GTGGCCTAAAGGGTCTGATA+TGG | 0.337002 | 6.1:-73994828 | None:intergenic |
| AGGTTGAAGATTCAGGTGTT+AGG | 0.346664 | 6.1:+73994985 | MS.gene98841:CDS |
| TGCCCTAAACCTATTCGATT+TGG | 0.350421 | 6.1:+73994790 | MS.gene98841:CDS |
| GAAGAGGGTGCCATGGAAAA+TGG | 0.350699 | 6.1:-73995598 | None:intergenic |
| AATTGCTATAGTGAAAGATT+TGG | 0.355756 | 6.1:+73995457 | MS.gene98841:CDS |
| AGGTGTTAGGGAAGATCTTC+TGG | 0.363892 | 6.1:+73994998 | MS.gene98841:CDS |
| TTAAAATACCTCAAAATTTG+TGG | 0.380720 | 6.1:-73994847 | None:intergenic |
| GACCAAATCGAATAGGTTTA+GGG | 0.384037 | 6.1:-73994792 | None:intergenic |
| CATTGCCAAATTGTTCATCT+TGG | 0.385266 | 6.1:-73995552 | None:intergenic |
| CCTCAAAATTTGTGGCCTAA+AGG | 0.407341 | 6.1:-73994839 | None:intergenic |
| TTTGTGGATCTCCACCTAGC+AGG | 0.415060 | 6.1:+73995512 | MS.gene98841:CDS |
| TGTGCTCCAATGAAGAAGTT+TGG | 0.423704 | 6.1:+73995643 | MS.gene98841:CDS |
| GGTTGAAGATTCAGGTGTTA+GGG | 0.425014 | 6.1:+73994986 | MS.gene98841:CDS |
| AGAGATGAGGATGGAATCTC+TGG | 0.450122 | 6.1:+73994765 | MS.gene98841:CDS |
| CAAGCTGCAGTGAGGATTGA+AGG | 0.451676 | 6.1:+73995673 | MS.gene98841:CDS |
| GTGTGACCAAACTTCTTCAT+TGG | 0.453010 | 6.1:-73995649 | None:intergenic |
| AATTGTTCATCTTGGATCAC+AGG | 0.459752 | 6.1:-73995544 | None:intergenic |
| TTTGGCAATGGCAATGGTCA+TGG | 0.463708 | 6.1:+73995565 | MS.gene98841:CDS |
| TTCATCAGACCAAATCGAAT+AGG | 0.466136 | 6.1:-73994799 | None:intergenic |
| GAAACCATATCAGACCCTTT+AGG | 0.483980 | 6.1:+73994824 | MS.gene98841:CDS |
| TTGTGGATCTCCACCTAGCA+GGG | 0.495640 | 6.1:+73995513 | MS.gene98841:CDS |
| TTGATTGTCTCAATAGGGAT+AGG | 0.498503 | 6.1:+73995698 | MS.gene98841:CDS |
| CATCTCTTCACAGCTTCTCA+TGG | 0.503294 | 6.1:-73994749 | None:intergenic |
| TCACCATCTTCCTCAGCTAG+AGG | 0.554835 | 6.1:+73995619 | MS.gene98841:CDS |
| TTGGCAATGGCAATGGTCAT+GGG | 0.558206 | 6.1:+73995566 | MS.gene98841:CDS |
| GAACAATTTGGCAATGGCAA+TGG | 0.561621 | 6.1:+73995559 | MS.gene98841:CDS |
| CAACCTCTAGCTGAGGAAGA+TGG | 0.571591 | 6.1:-73995622 | None:intergenic |
| ATTGTTCATCTTGGATCACA+GGG | 0.573361 | 6.1:-73995543 | None:intergenic |
| AAGCTGTGAAGAGATGAGGA+TGG | 0.577435 | 6.1:+73994756 | MS.gene98841:CDS |
| AGCTGAGGAAGATGGTGAAG+AGG | 0.581033 | 6.1:-73995614 | None:intergenic |
| CAAGATGAACAATTTGGCAA+TGG | 0.587612 | 6.1:+73995553 | MS.gene98841:CDS |
| AGATGGTGAAGAGGGTGCCA+TGG | 0.590017 | 6.1:-73995605 | None:intergenic |
| ACAGGGTTTGAAGCCCTGCT+AGG | 0.591659 | 6.1:-73995526 | None:intergenic |
| GGGTTTGAAGCCCTGCTAGG+TGG | 0.606726 | 6.1:-73995523 | None:intergenic |
| GCTGAGGAAGATGGTGAAGA+GGG | 0.607679 | 6.1:-73995613 | None:intergenic |
| TCTGGATATCATTCTCCCCA+AGG | 0.612920 | 6.1:+73995016 | MS.gene98841:CDS |
| TTGCTATAGTGAAAGATTTG+GGG | 0.631998 | 6.1:+73995459 | MS.gene98841:CDS |
| AATAATCAGCTATCAGTCAG+AGG | 0.650098 | 6.1:+73994965 | MS.gene98841:intron |
| TGGAGCACAACCTCTAGCTG+AGG | 0.654183 | 6.1:-73995629 | None:intergenic |
| TGAGAAGCTGTGAAGAGATG+AGG | 0.665577 | 6.1:+73994752 | MS.gene98841:CDS |
| GTCACACACAAGCTGCAGTG+AGG | 0.704483 | 6.1:+73995665 | MS.gene98841:CDS |
| TGTGAAAAGATGAATCACTG+TGG | 0.839717 | 6.1:+73994709 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTACAAAAACAATTGA+TGG | - | chr6.1:73994919-73994938 | None:intergenic | 15.0% |
| !!! | CTTAATTTTTCATGAAAATA+AGG | + | chr6.1:73995057-73995076 | MS.gene98841:intron | 15.0% |
| !! | ATGTTTAATGAACTTAGTTA+AGG | + | chr6.1:73995214-73995233 | MS.gene98841:intron | 20.0% |
| !! | TTAAAATACCTCAAAATTTG+TGG | - | chr6.1:73994850-73994869 | None:intergenic | 20.0% |
| !!! | AACAATGATTTGTTTTGATT+TGG | + | chr6.1:73995422-73995441 | MS.gene98841:intron | 20.0% |
| !!! | ACAAATTTTGAGGTATTTTA+AGG | + | chr6.1:73994849-73994868 | MS.gene98841:intron | 20.0% |
| !!! | CAAATTTTGAGGTATTTTAA+GGG | + | chr6.1:73994850-73994869 | MS.gene98841:intron | 20.0% |
| ! | AATTGCTATAGTGAAAGATT+TGG | + | chr6.1:73995457-73995476 | MS.gene98841:CDS | 25.0% |
| ! | ATTGCTATAGTGAAAGATTT+GGG | + | chr6.1:73995458-73995477 | MS.gene98841:CDS | 25.0% |
| ! | TTGACATAGAAATTTACCTT+GGG | - | chr6.1:73995035-73995054 | None:intergenic | 25.0% |
| ! | TTTGACATAGAAATTTACCT+TGG | - | chr6.1:73995036-73995055 | None:intergenic | 25.0% |
| !! | TCAATTCAATTACCATGTTT+TGG | + | chr6.1:73995094-73995113 | MS.gene98841:intron | 25.0% |
| CATCAATCATAACCAAAACA+TGG | - | chr6.1:73995109-73995128 | None:intergenic | 30.0% | |
| CCTTCTGACTGAAATAAAAT+CGG | + | chr6.1:73995271-73995290 | MS.gene98841:intron | 30.0% | |
| TGACATAGAAATTTACCTTG+GGG | - | chr6.1:73995034-73995053 | None:intergenic | 30.0% | |
| TTGCTATAGTGAAAGATTTG+GGG | + | chr6.1:73995459-73995478 | MS.gene98841:CDS | 30.0% | |
| !! | GAATTTTAGTCCATTTTCCA+TGG | + | chr6.1:73995588-73995607 | MS.gene98841:CDS | 30.0% |
| !!! | AAGGTTTTGATTGTCTCAAT+AGG | + | chr6.1:73995692-73995711 | MS.gene98841:CDS | 30.0% |
| !!! | AGGTTTTGATTGTCTCAATA+GGG | + | chr6.1:73995693-73995712 | MS.gene98841:CDS | 30.0% |
| !!! | GTTTTGATTTGGATTTTGCA+GGG | + | chr6.1:73995433-73995452 | MS.gene98841:intron | 30.0% |
| !!! | TGTTTTGATTTGGATTTTGC+AGG | + | chr6.1:73995432-73995451 | MS.gene98841:intron | 30.0% |
| AATAATCAGCTATCAGTCAG+AGG | + | chr6.1:73994965-73994984 | MS.gene98841:intron | 35.0% | |
| AATTGTTCATCTTGGATCAC+AGG | - | chr6.1:73995547-73995566 | None:intergenic | 35.0% | |
| AGACCAAATCGAATAGGTTT+AGG | - | chr6.1:73994796-73994815 | None:intergenic | 35.0% | |
| ATTCTCATGGAAAATCGTCA+TGG | - | chr6.1:73995324-73995343 | None:intergenic | 35.0% | |
| ATTGTTCATCTTGGATCACA+GGG | - | chr6.1:73995546-73995565 | None:intergenic | 35.0% | |
| CAAGATGAACAATTTGGCAA+TGG | + | chr6.1:73995553-73995572 | MS.gene98841:CDS | 35.0% | |
| CAGTCAGAAGGTAATAAAGT+CGG | - | chr6.1:73995262-73995281 | None:intergenic | 35.0% | |
| CATTGCCAAATTGTTCATCT+TGG | - | chr6.1:73995555-73995574 | None:intergenic | 35.0% | |
| CTCAAAATTTGTGGCCTAAA+GGG | - | chr6.1:73994841-73994860 | None:intergenic | 35.0% | |
| GACCAAATCGAATAGGTTTA+GGG | - | chr6.1:73994795-73994814 | None:intergenic | 35.0% | |
| GTGATCCAAGATGAACAATT+TGG | + | chr6.1:73995547-73995566 | MS.gene98841:CDS | 35.0% | |
| TGTTGTTGAATCACTTGATC+TGG | + | chr6.1:73995186-73995205 | MS.gene98841:intron | 35.0% | |
| TTCATCAGACCAAATCGAAT+AGG | - | chr6.1:73994802-73994821 | None:intergenic | 35.0% | |
| TTGATTGTCTCAATAGGGAT+AGG | + | chr6.1:73995698-73995717 | MS.gene98841:CDS | 35.0% | |
| ! | GATTTTCCATGAGAATCGAA+GGG | + | chr6.1:73995328-73995347 | MS.gene98841:intron | 35.0% |
| !! | CCGATTTTATTTCAGTCAGA+AGG | - | chr6.1:73995274-73995293 | None:intergenic | 35.0% |
| AGGTTGAAGATTCAGGTGTT+AGG | + | chr6.1:73994985-73995004 | MS.gene98841:CDS | 40.0% | |
| CCTCAAAATTTGTGGCCTAA+AGG | - | chr6.1:73994842-73994861 | None:intergenic | 40.0% | |
| GAAACCATATCAGACCCTTT+AGG | + | chr6.1:73994824-73994843 | MS.gene98841:CDS | 40.0% | |
| GAAAGATTTGGGGAGCAATT+AGG | + | chr6.1:73995469-73995488 | MS.gene98841:CDS | 40.0% | |
| GAACAATTTGGCAATGGCAA+TGG | + | chr6.1:73995559-73995578 | MS.gene98841:CDS | 40.0% | |
| GATCCACAAAAAAATGGTGG+TGG | - | chr6.1:73995502-73995521 | None:intergenic | 40.0% | |
| GGAGATCCACAAAAAAATGG+TGG | - | chr6.1:73995505-73995524 | None:intergenic | 40.0% | |
| GGTGGAGATCCACAAAAAAA+TGG | - | chr6.1:73995508-73995527 | None:intergenic | 40.0% | |
| GGTTGAAGATTCAGGTGTTA+GGG | + | chr6.1:73994986-73995005 | MS.gene98841:CDS | 40.0% | |
| GTGTGACCAAACTTCTTCAT+TGG | - | chr6.1:73995652-73995671 | None:intergenic | 40.0% | |
| TAGGCTGGAAAAAAGAAGCT+TGG | + | chr6.1:73995353-73995372 | MS.gene98841:intron | 40.0% | |
| TGCCCTAAACCTATTCGATT+TGG | + | chr6.1:73994790-73994809 | MS.gene98841:CDS | 40.0% | |
| TGTGCTCCAATGAAGAAGTT+TGG | + | chr6.1:73995643-73995662 | MS.gene98841:CDS | 40.0% | |
| ! | CCTTTAGGCCACAAATTTTG+AGG | + | chr6.1:73994839-73994858 | MS.gene98841:CDS | 40.0% |
| ! | CGATTTTCCATGAGAATCGA+AGG | + | chr6.1:73995327-73995346 | MS.gene98841:intron | 40.0% |
| !!! | TCACCACCACCATTTTTTTG+TGG | + | chr6.1:73995496-73995515 | MS.gene98841:CDS | 40.0% |
| AAGCTGTGAAGAGATGAGGA+TGG | + | chr6.1:73994756-73994775 | MS.gene98841:CDS | 45.0% | |
| AGAGATGAGGATGGAATCTC+TGG | + | chr6.1:73994765-73994784 | MS.gene98841:CDS | 45.0% | |
| AGGTGTTAGGGAAGATCTTC+TGG | + | chr6.1:73994998-73995017 | MS.gene98841:CDS | 45.0% | |
| CAGTCAGAGGTTGAAGATTC+AGG | + | chr6.1:73994978-73994997 | MS.gene98841:CDS | 45.0% | |
| CATCTCTTCACAGCTTCTCA+TGG | - | chr6.1:73994752-73994771 | None:intergenic | 45.0% | |
| CCATGAGAATCGAAGGGTTT+AGG | + | chr6.1:73995334-73995353 | MS.gene98841:intron | 45.0% | |
| CCTAAACCCTTCGATTCTCA+TGG | - | chr6.1:73995337-73995356 | None:intergenic | 45.0% | |
| TCTGGATATCATTCTCCCCA+AGG | + | chr6.1:73995016-73995035 | MS.gene98841:CDS | 45.0% | |
| TGAGAAGCTGTGAAGAGATG+AGG | + | chr6.1:73994752-73994771 | MS.gene98841:CDS | 45.0% | |
| TTGGCAATGGCAATGGTCAT+GGG | + | chr6.1:73995566-73995585 | MS.gene98841:CDS | 45.0% | |
| TTTGGCAATGGCAATGGTCA+TGG | + | chr6.1:73995565-73995584 | MS.gene98841:CDS | 45.0% | |
| AGCTGAGGAAGATGGTGAAG+AGG | - | chr6.1:73995617-73995636 | None:intergenic | 50.0% | |
| CAACCTCTAGCTGAGGAAGA+TGG | - | chr6.1:73995625-73995644 | None:intergenic | 50.0% | |
| CAAGCTGCAGTGAGGATTGA+AGG | + | chr6.1:73995673-73995692 | MS.gene98841:CDS | 50.0% | |
| GAGAATCGAAGGGTTTAGGC+TGG | + | chr6.1:73995338-73995357 | MS.gene98841:intron | 50.0% | |
| GCTGAGGAAGATGGTGAAGA+GGG | - | chr6.1:73995616-73995635 | None:intergenic | 50.0% | |
| GTGGCCTAAAGGGTCTGATA+TGG | - | chr6.1:73994831-73994850 | None:intergenic | 50.0% | |
| TCACCATCTTCCTCAGCTAG+AGG | + | chr6.1:73995619-73995638 | MS.gene98841:CDS | 50.0% | |
| TTGTGGATCTCCACCTAGCA+GGG | + | chr6.1:73995513-73995532 | MS.gene98841:CDS | 50.0% | |
| TTTGTGGATCTCCACCTAGC+AGG | + | chr6.1:73995512-73995531 | MS.gene98841:CDS | 50.0% | |
| !! | GAAGAGGGTGCCATGGAAAA+TGG | - | chr6.1:73995601-73995620 | None:intergenic | 50.0% |
| GTCACACACAAGCTGCAGTG+AGG | + | chr6.1:73995665-73995684 | MS.gene98841:CDS | 55.0% | |
| TGGAGCACAACCTCTAGCTG+AGG | - | chr6.1:73995632-73995651 | None:intergenic | 55.0% | |
| ! | ACAGGGTTTGAAGCCCTGCT+AGG | - | chr6.1:73995529-73995548 | None:intergenic | 55.0% |
| !! | AGATGGTGAAGAGGGTGCCA+TGG | - | chr6.1:73995608-73995627 | None:intergenic | 55.0% |
| ! | GGGTTTGAAGCCCTGCTAGG+TGG | - | chr6.1:73995526-73995545 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 73994718 | 73995750 | 73994718 | ID=MS.gene98841 |
| chr6.1 | mRNA | 73994718 | 73995750 | 73994718 | ID=MS.gene98841.t1;Parent=MS.gene98841 |
| chr6.1 | exon | 73994718 | 73994860 | 73994718 | ID=MS.gene98841.t1.exon1;Parent=MS.gene98841.t1 |
| chr6.1 | CDS | 73994718 | 73994860 | 73994718 | ID=cds.MS.gene98841.t1;Parent=MS.gene98841.t1 |
| chr6.1 | exon | 73994974 | 73995037 | 73994974 | ID=MS.gene98841.t1.exon2;Parent=MS.gene98841.t1 |
| chr6.1 | CDS | 73994974 | 73995037 | 73994974 | ID=cds.MS.gene98841.t1;Parent=MS.gene98841.t1 |
| chr6.1 | exon | 73995454 | 73995750 | 73995454 | ID=MS.gene98841.t1.exon3;Parent=MS.gene98841.t1 |
| chr6.1 | CDS | 73995454 | 73995750 | 73995454 | ID=cds.MS.gene98841.t1;Parent=MS.gene98841.t1 |
| Gene Sequence |
| Protein sequence |
