AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99721

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99721.t1 MTR_5g074200 92.308 182 14 0 1 182 146 327 8.61e-125 354
MS.gene99721.t1 MTR_5g093540 59.341 182 22 3 1 182 120 249 1.52e-61 191
MS.gene99721.t1 MTR_5g074400 52.083 144 57 5 47 182 213 352 6.13e-34 126
MS.gene99721.t1 MTR_5g074400 56.452 62 26 1 75 135 420 481 2.14e-16 76.6
MS.gene99721.t1 MTR_5g073850 66.265 83 26 2 1 82 78 159 1.39e-31 112
MS.gene99721.t1 MTR_3g031220 50.388 129 58 3 55 182 178 301 1.71e-29 112
MS.gene99721.t1 MTR_3g031220 50.388 129 58 3 55 182 178 301 3.12e-29 112
MS.gene99721.t1 MTR_3g031220 47.436 78 37 2 75 151 365 439 2.04e-14 70.9
MS.gene99721.t1 MTR_7g071120 55.814 86 34 2 57 141 150 232 5.24e-25 100
MS.gene99721.t1 MTR_7g071120 44.944 89 39 3 75 156 324 409 3.11e-15 73.2
MS.gene99721.t1 MTR_7g100510 66.176 68 22 1 76 143 190 256 1.15e-24 99.8
MS.gene99721.t1 MTR_7g100510 52.542 59 27 1 76 133 362 420 6.16e-13 66.2
MS.gene99721.t1 MTR_4g013260 48.936 94 45 3 56 147 164 256 4.94e-22 92.4
MS.gene99721.t1 MTR_4g013260 53.333 60 27 1 75 133 336 395 1.01e-13 68.6
MS.gene99721.t1 MTR_3g056100 60.938 64 25 0 75 138 243 306 5.80e-22 92.4
MS.gene99721.t1 MTR_3g056100 53.226 62 28 1 75 135 402 463 2.14e-15 73.6
MS.gene99721.t1 MTR_7g080020 64.062 64 23 0 75 138 280 343 6.09e-22 92.4
MS.gene99721.t1 MTR_7g080020 51.613 62 29 1 75 135 493 554 2.85e-14 70.5
MS.gene99721.t1 MTR_3g056100 60.938 64 25 0 75 138 243 306 6.09e-22 92.4
MS.gene99721.t1 MTR_3g056100 53.226 62 28 1 75 135 415 476 2.28e-15 73.6
MS.gene99721.t1 MTR_3g056100 60.938 64 25 0 75 138 99 162 7.78e-22 91.7
MS.gene99721.t1 MTR_3g056100 53.226 62 28 1 75 135 258 319 3.43e-15 72.8
MS.gene99721.t1 MTR_3g056100 60.938 64 25 0 75 138 99 162 9.50e-22 91.7
MS.gene99721.t1 MTR_3g056100 53.226 62 28 1 75 135 271 332 3.89e-15 72.8
MS.gene99721.t1 MTR_7g011820 57.895 76 32 0 58 133 211 286 9.56e-22 91.7
MS.gene99721.t1 MTR_7g011820 44.898 98 43 4 75 165 410 503 1.63e-15 73.9
MS.gene99721.t1 MTR_5g094430 43.519 108 51 2 36 143 171 268 1.30e-21 91.3
MS.gene99721.t1 MTR_5g094430 51.613 62 29 1 75 135 364 425 1.24e-14 71.2
MS.gene99721.t1 MTR_5g094430 46.392 97 43 1 47 143 69 156 1.71e-21 90.9
MS.gene99721.t1 MTR_5g094430 51.613 62 29 1 75 135 252 313 1.59e-14 70.9
MS.gene99721.t1 MTR_5g094430 46.392 97 43 1 47 143 47 134 1.73e-21 90.5
MS.gene99721.t1 MTR_5g094430 51.613 62 29 1 75 135 230 291 1.62e-14 70.9
MS.gene99721.t1 MTR_5g042920 64.407 59 21 0 75 133 151 209 4.29e-21 89.7
MS.gene99721.t1 MTR_5g042920 53.226 62 28 1 75 135 320 381 3.37e-15 72.8
MS.gene99721.t1 MTR_3g009470 60.938 64 25 0 75 138 280 343 4.87e-21 89.7
MS.gene99721.t1 MTR_3g009470 53.226 62 28 1 75 135 495 556 2.97e-14 70.5
MS.gene99721.t1 MTR_3g009470 60.938 64 25 0 75 138 280 343 9.64e-21 89.0
MS.gene99721.t1 MTR_7g080000 60.317 63 25 0 76 138 235 297 9.35e-20 86.3
MS.gene99721.t1 MTR_7g080000 51.613 62 29 1 75 135 432 493 2.88e-14 70.5
MS.gene99721.t1 MTR_4g082580 44.444 108 55 2 41 143 107 214 3.70e-19 84.0
MS.gene99721.t1 MTR_4g082580 51.613 62 29 1 75 135 278 339 1.72e-14 70.9
MS.gene99721.t1 MTR_1g077780 49.296 71 35 1 75 144 62 132 1.69e-16 72.8
MS.gene99721.t1 MTR_6g046600 64.151 53 18 1 91 143 18 69 5.92e-16 70.5
MS.gene99721.t1 MTR_5g097490 30.286 175 90 3 5 158 1 164 1.11e-15 73.9
MS.gene99721.t1 MTR_5g097490 45.902 61 32 1 75 134 252 312 5.07e-13 66.6
MS.gene99721.t1 MTR_4g094588 43.011 93 46 3 75 166 178 264 1.56e-14 70.5
MS.gene99721.t1 MTR_3g106060 42.222 90 45 5 76 158 139 228 3.58e-14 69.3
MS.gene99721.t1 MTR_5g097500 46.053 76 41 0 62 137 70 145 5.14e-14 68.9
MS.gene99721.t1 MTR_5g097500 40.580 69 40 1 75 142 230 298 3.73e-12 63.5
MS.gene99721.t1 MTR_2g033820 49.206 63 31 1 75 136 203 265 7.58e-14 68.9
MS.gene99721.t1 MTR_1g086790 49.206 63 31 1 75 136 158 220 8.31e-14 68.6
MS.gene99721.t1 MTR_5g091390 48.387 62 31 1 75 135 157 218 8.72e-14 68.2
MS.gene99721.t1 MTR_3g095080 41.558 77 43 2 75 150 168 243 9.78e-14 67.4
MS.gene99721.t1 MTR_3g095080 41.558 77 43 2 75 150 144 219 1.10e-13 67.0
MS.gene99721.t1 MTR_8g007270 56.140 57 24 1 75 130 154 210 1.44e-13 66.6
MS.gene99721.t1 MTR_2g105060 38.095 84 49 3 76 156 95 178 1.60e-13 66.6
MS.gene99721.t1 MTR_8g092140 52.632 57 26 1 75 130 160 216 1.73e-13 66.6
MS.gene99721.t1 MTR_2g105060 38.095 84 49 3 76 156 170 253 2.45e-13 67.0
MS.gene99721.t1 MTR_7g109800 46.154 78 38 2 75 151 145 219 3.84e-13 66.2
MS.gene99721.t1 MTR_4g122530 50.000 60 29 1 75 133 208 267 3.88e-13 67.0
MS.gene99721.t1 MTR_8g096730 49.123 57 28 1 75 130 70 126 3.92e-13 63.9
MS.gene99721.t1 MTR_4g094412 49.180 61 29 2 75 133 234 294 4.56e-13 66.6
MS.gene99721.t1 MTR_4g094412 49.180 61 29 2 75 133 243 303 4.77e-13 66.6
MS.gene99721.t1 MTR_4g107970 47.541 61 30 2 75 133 178 238 8.07e-13 66.2
MS.gene99721.t1 MTR_1g015140 36.792 106 61 3 26 130 58 158 9.08e-13 63.2
MS.gene99721.t1 MTR_3g104750 49.123 57 28 1 75 130 104 160 9.14e-13 63.5
MS.gene99721.t1 MTR_4g107970 47.541 61 30 2 75 133 255 315 9.82e-13 65.9
MS.gene99721.t1 MTR_3g053330 32.143 140 89 4 44 181 222 357 1.36e-12 65.5
MS.gene99721.t1 MTR_4g007060 45.000 60 31 2 76 133 129 188 1.47e-12 64.7
MS.gene99721.t1 MTR_8g092010 39.091 110 54 4 38 136 63 170 1.67e-12 63.2
MS.gene99721.t1 MTR_4g007060 45.000 60 31 2 76 133 163 222 1.76e-12 64.7
MS.gene99721.t1 MTR_7g079010 50.877 57 27 1 75 130 141 197 1.82e-12 63.5
MS.gene99721.t1 MTR_4g132430 43.077 65 35 2 75 137 127 191 3.81e-12 63.9
MS.gene99721.t1 MTR_1g013790 33.684 95 55 3 57 143 77 171 5.20e-12 62.8
MS.gene99721.t1 MTR_7g028415 50.877 57 27 1 75 130 93 149 6.16e-12 61.2
MS.gene99721.t1 MTR_7g028710 50.877 57 27 1 75 130 93 149 6.16e-12 61.2
MS.gene99721.t1 MTR_7g062220 50.000 58 28 1 75 131 124 181 6.77e-12 63.2
MS.gene99721.t1 MTR_7g009730 48.276 58 28 2 75 130 219 276 1.02e-11 62.8
MS.gene99721.t1 MTR_4g132430 43.077 65 35 2 75 137 261 325 1.10e-11 62.8
MS.gene99721.t1 MTR_3g095040 49.206 63 31 1 75 136 118 180 1.68e-11 60.8
MS.gene99721.t1 MTR_2g045360 40.000 80 45 3 76 152 212 291 2.14e-11 62.0
MS.gene99721.t1 MTR_4g130900 44.068 59 32 1 76 133 360 418 3.77e-11 61.2
MS.gene99721.t1 MTR_5g074080 62.500 48 16 1 103 150 71 116 4.78e-11 58.5
MS.gene99721.t1 MTR_2g024350 50.794 63 27 3 76 135 9 70 5.12e-11 59.7
MS.gene99721.t1 MTR_5g043880 49.123 57 28 1 75 130 124 180 7.81e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99721.t1 AT5G07100 64.286 70 23 2 75 143 24 92 2.77e-24 95.1
MS.gene99721.t1 AT5G07100 54.098 61 27 1 75 134 141 201 1.61e-15 72.0
MS.gene99721.t1 AT5G07100 64.286 70 23 2 75 143 24 92 2.77e-24 95.1
MS.gene99721.t1 AT5G07100 54.098 61 27 1 75 134 141 201 1.61e-15 72.0
MS.gene99721.t1 AT5G07100 64.286 70 23 2 75 143 24 92 2.77e-24 95.1
MS.gene99721.t1 AT5G07100 54.098 61 27 1 75 134 141 201 1.61e-15 72.0
MS.gene99721.t1 AT5G07100 64.286 70 23 2 75 143 24 92 2.77e-24 95.1
MS.gene99721.t1 AT5G07100 54.098 61 27 1 75 134 141 201 1.61e-15 72.0
MS.gene99721.t1 AT5G07100 64.286 70 23 2 75 143 117 185 3.34e-24 96.7
MS.gene99721.t1 AT5G07100 54.098 61 27 1 75 134 234 294 1.37e-15 73.2
MS.gene99721.t1 AT2G38470 66.102 59 20 0 75 133 184 242 7.29e-24 97.8
MS.gene99721.t1 AT2G38470 53.226 62 28 1 75 135 362 423 5.54e-14 69.3
MS.gene99721.t1 AT1G13960 62.121 66 25 0 75 140 229 294 2.42e-23 96.3
MS.gene99721.t1 AT1G13960 56.140 57 24 1 75 130 409 465 1.75e-15 73.9
MS.gene99721.t1 AT1G13960 62.121 66 25 0 75 140 76 141 3.67e-23 94.7
MS.gene99721.t1 AT1G13960 56.140 57 24 1 75 130 256 312 4.94e-15 72.0
MS.gene99721.t1 AT2G37260 60.294 68 27 0 76 143 166 233 7.12e-23 94.7
MS.gene99721.t1 AT2G37260 39.759 83 43 2 49 130 329 405 8.39e-12 63.2
MS.gene99721.t1 AT2G37260 60.294 68 27 0 76 143 84 151 7.50e-23 93.6
MS.gene99721.t1 AT2G37260 39.759 83 43 2 49 130 247 323 1.54e-11 62.0
MS.gene99721.t1 AT2G37260 60.294 68 27 0 76 143 86 153 7.83e-23 93.6
MS.gene99721.t1 AT2G37260 39.759 83 43 2 49 130 249 325 1.71e-11 62.0
MS.gene99721.t1 AT5G56270 66.102 59 20 0 75 133 273 331 2.77e-22 93.6
MS.gene99721.t1 AT5G56270 54.839 62 27 1 75 135 487 548 8.19e-16 74.7
MS.gene99721.t1 AT2G03340 60.294 68 27 0 75 142 250 317 6.36e-22 92.4
MS.gene99721.t1 AT2G03340 53.226 62 28 1 75 135 415 476 1.16e-15 74.3
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 142 236 8.34e-22 92.0
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 341 424 2.01e-15 73.6
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 182 276 1.04e-21 91.7
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 381 464 2.02e-15 73.6
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 110 204 1.21e-21 91.3
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 309 392 2.24e-15 73.6
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 6 100 1.54e-21 90.5
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 205 288 2.22e-15 73.2
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 6 100 1.54e-21 90.5
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 205 288 2.22e-15 73.2
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 6 100 1.54e-21 90.5
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 205 288 2.22e-15 73.2
MS.gene99721.t1 AT4G26640 50.000 96 37 2 56 141 6 100 1.54e-21 90.5
MS.gene99721.t1 AT4G26640 46.512 86 43 2 75 159 205 288 2.22e-15 73.2
MS.gene99721.t1 AT2G04880 51.351 74 36 0 60 133 96 169 2.39e-21 90.5
MS.gene99721.t1 AT2G04880 46.479 71 35 2 64 133 274 342 1.77e-12 65.1
MS.gene99721.t1 AT2G04880 51.351 74 36 0 60 133 96 169 2.41e-21 90.5
MS.gene99721.t1 AT2G04880 46.479 71 35 2 64 133 298 366 1.59e-12 65.1
MS.gene99721.t1 AT4G26440 64.407 59 21 0 75 133 178 236 1.28e-20 88.6
MS.gene99721.t1 AT4G26440 52.174 69 29 2 75 142 372 437 1.08e-13 68.6
MS.gene99721.t1 AT3G01080 61.017 59 23 0 75 133 205 263 2.80e-20 86.3
MS.gene99721.t1 AT2G30250 48.780 82 36 1 73 154 164 239 1.17e-19 85.5
MS.gene99721.t1 AT2G30250 54.098 61 27 1 76 135 329 389 7.42e-15 71.6
MS.gene99721.t1 AT3G01080 61.017 59 23 0 75 133 205 263 1.52e-19 85.5
MS.gene99721.t1 AT3G01080 56.667 60 25 1 75 133 344 403 5.38e-17 78.2
MS.gene99721.t1 AT3G01080 61.017 59 23 0 75 133 167 225 2.01e-19 84.7
MS.gene99721.t1 AT3G01080 56.667 60 25 1 75 133 306 365 7.58e-17 77.4
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 3.65e-17 79.0
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 3.75e-17 79.0
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 3.96e-17 79.0
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 3.96e-17 79.0
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 4.38e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 4.49e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 4.59e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 4.67e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 4.67e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 390 470 4.76e-17 78.6
MS.gene99721.t1 AT4G12020 47.059 85 41 2 62 146 457 537 5.18e-17 78.6
MS.gene99721.t1 AT2G25000 35.354 99 58 3 44 140 76 170 6.42e-16 73.2
MS.gene99721.t1 AT2G25000 35.354 99 58 3 44 140 76 170 6.42e-16 73.2
MS.gene99721.t1 AT2G25000 35.714 98 57 3 44 139 98 191 1.27e-15 72.8
MS.gene99721.t1 AT2G25000 35.714 98 57 3 44 139 119 212 2.13e-15 72.4
MS.gene99721.t1 AT1G55600 45.000 80 38 2 62 135 289 368 3.17e-15 73.2
MS.gene99721.t1 AT5G13080 52.632 57 26 1 75 130 67 123 1.10e-14 68.2
MS.gene99721.t1 AT2G44745 37.815 119 55 3 31 130 83 201 1.56e-14 69.3
MS.gene99721.t1 AT1G29860 45.205 73 38 2 75 146 136 207 1.08e-13 67.8
MS.gene99721.t1 AT4G18170 52.459 61 28 1 75 134 172 232 1.19e-13 68.2
MS.gene99721.t1 AT2G47260 39.560 91 48 2 75 158 174 264 2.94e-13 67.0
MS.gene99721.t1 AT1G64000 50.877 57 27 1 75 130 114 170 3.55e-13 65.1
MS.gene99721.t1 AT5G49520 50.000 62 30 1 73 133 219 280 5.55e-13 66.6
MS.gene99721.t1 AT5G41570 50.877 57 27 1 75 130 98 154 5.89e-13 64.3
MS.gene99721.t1 AT2G21900 46.377 69 36 1 67 134 101 169 5.97e-13 64.7
MS.gene99721.t1 AT3G62340 36.842 114 58 2 75 174 118 231 6.57e-13 65.5
MS.gene99721.t1 AT4G31800 42.857 63 34 2 76 136 112 174 6.88e-13 65.1
MS.gene99721.t1 AT4G04450 40.260 77 44 2 76 150 199 275 7.35e-13 66.2
MS.gene99721.t1 AT4G04450 40.260 77 44 2 76 150 293 369 9.90e-13 65.9
MS.gene99721.t1 AT5G46350 50.820 61 29 1 75 134 183 243 1.18e-12 65.1
MS.gene99721.t1 AT4G31800 42.857 63 34 2 76 136 177 239 1.22e-12 65.1
MS.gene99721.t1 AT2G46130 50.877 57 27 1 75 130 30 86 1.34e-12 61.6
MS.gene99721.t1 AT1G68150 42.857 77 39 3 75 146 235 311 2.07e-12 64.7
MS.gene99721.t1 AT4G39410 43.590 78 41 3 75 150 223 299 2.11e-12 64.3
MS.gene99721.t1 AT1G80840 45.902 61 31 2 76 134 147 207 3.62e-12 63.5
MS.gene99721.t1 AT5G26170 49.123 57 28 1 75 130 113 169 3.87e-12 62.0
MS.gene99721.t1 AT5G15130 42.254 71 39 2 75 143 185 255 4.14e-12 63.9
MS.gene99721.t1 AT5G15130 42.254 71 39 2 75 143 227 297 4.23e-12 63.9
MS.gene99721.t1 AT5G43290 53.448 58 26 1 75 131 114 171 5.39e-12 63.2
MS.gene99721.t1 AT3G01970 50.877 57 27 1 75 130 65 121 1.37e-11 60.1
MS.gene99721.t1 AT5G64810 49.123 57 28 1 75 130 110 166 1.80e-11 60.5
MS.gene99721.t1 AT4G30935 36.486 74 46 1 76 148 332 405 1.82e-11 62.0
MS.gene99721.t1 AT4G30935 38.095 84 50 2 51 133 144 226 6.54e-11 60.5
MS.gene99721.t1 AT1G69310 49.153 59 29 1 73 130 145 203 1.90e-11 61.6
MS.gene99721.t1 AT1G69310 49.153 59 29 1 73 130 145 203 1.90e-11 61.6
MS.gene99721.t1 AT1G69310 49.153 59 29 1 73 130 145 203 1.90e-11 61.6
MS.gene99721.t1 AT4G01720 44.262 61 32 2 75 133 239 299 9.81e-11 60.1

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTTCTTCGTTCGAAAT+TGG 0.147163 5.2:+62122758 MS.gene99721:CDS
ATCATGATGATGGGTATAAT+TGG 0.315840 5.2:+62122922 MS.gene99721:CDS
ATTGATACTGAGGACTTTGC+AGG 0.337079 5.2:-62123227 None:intergenic
ATCATTTGGAAGTGAACAAT+TGG 0.346812 5.2:+62123190 MS.gene99721:CDS
ATTGTTCTTCGTTCGAAATT+GGG 0.351681 5.2:+62122759 MS.gene99721:CDS
TTGGGTTCGAGGCATGAAAT+AGG 0.377257 5.2:+62122777 MS.gene99721:CDS
GACTTGCAAGATCAATCATT+TGG 0.392633 5.2:+62123176 MS.gene99721:CDS
TAATTGTTTAGTGAAGAAAA+AGG 0.403951 5.2:+62123013 MS.gene99721:CDS
TTCCTCTTCATTGAAGAAGT+TGG 0.422483 5.2:-62123101 None:intergenic
TGATTGATCTTGCAAGTCAA+TGG 0.431766 5.2:-62123171 None:intergenic
TATGAAGATAAAGTAGCAAA+AGG 0.433124 5.2:+62122951 MS.gene99721:CDS
GATTGATCTTGCAAGTCAAT+GGG 0.437794 5.2:-62123170 None:intergenic
AAGGTTGAGAGAACAATAGA+TGG 0.440547 5.2:+62123032 MS.gene99721:CDS
TTCGTTCGAAATTGGGTTCG+AGG 0.448339 5.2:+62122766 MS.gene99721:CDS
TTTCAATCTGATTATAGTAA+TGG 0.456610 5.2:+62122864 MS.gene99721:CDS
AAAATCAGTCTTTGATAAGA+AGG 0.457061 5.2:+62122895 MS.gene99721:CDS
GATGTAGCTATTGAAATTAA+TGG 0.458940 5.2:+62122819 MS.gene99721:CDS
ACAAAGGTACACATAATCAT+TGG 0.464789 5.2:+62123075 MS.gene99721:CDS
AGAAGGATGAATCATGATGA+TGG 0.481196 5.2:+62122912 MS.gene99721:CDS
AGCCAACTTCTTCAATGAAG+AGG 0.565986 5.2:+62123099 MS.gene99721:CDS
GAAGGATGAATCATGATGAT+GGG 0.572110 5.2:+62122913 MS.gene99721:CDS
AACATTTCATATTGATACTG+AGG 0.591858 5.2:-62123237 None:intergenic
TCAATGGGGCCAATCTCTGA+AGG 0.606884 5.2:-62123155 None:intergenic
ATTGATCTTGCAAGTCAATG+GGG 0.684106 5.2:-62123169 None:intergenic
GTTATTGAGACACTATACAA+AGG 0.697342 5.2:+62123059 MS.gene99721:CDS
GAAGCTATTATAAATGTACG+TGG 0.742371 5.2:+62122988 MS.gene99721:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTGTTTAGTGAAGAAAA+AGG + chr5.2:62123013-62123032 MS.gene99721:CDS 20.0%
!! TTTCAATCTGATTATAGTAA+TGG + chr5.2:62122864-62122883 MS.gene99721:CDS 20.0%
!!! TTTTTCTTCACTAAACAATT+AGG - chr5.2:62123014-62123033 None:intergenic 20.0%
! AAAATCAGTCTTTGATAAGA+AGG + chr5.2:62122895-62122914 MS.gene99721:CDS 25.0%
! GATGTAGCTATTGAAATTAA+TGG + chr5.2:62122819-62122838 MS.gene99721:CDS 25.0%
! TATGAAGATAAAGTAGCAAA+AGG + chr5.2:62122951-62122970 MS.gene99721:CDS 25.0%
!!! TTATCAAAGACTGATTTTGT+TGG - chr5.2:62122893-62122912 None:intergenic 25.0%
ATCATTTGGAAGTGAACAAT+TGG + chr5.2:62123190-62123209 MS.gene99721:CDS 30.0%
ATTGTTCTTCGTTCGAAATT+GGG + chr5.2:62122759-62122778 MS.gene99721:CDS 30.0%
GAAGCTATTATAAATGTACG+TGG + chr5.2:62122988-62123007 MS.gene99721:CDS 30.0%
GTTATTGAGACACTATACAA+AGG + chr5.2:62123059-62123078 MS.gene99721:CDS 30.0%
TATTGTTCTTCGTTCGAAAT+TGG + chr5.2:62122758-62122777 MS.gene99721:CDS 30.0%
! ACAAAGGTACACATAATCAT+TGG + chr5.2:62123075-62123094 MS.gene99721:CDS 30.0%
!! ATCATGATGATGGGTATAAT+TGG + chr5.2:62122922-62122941 MS.gene99721:CDS 30.0%
AAGGTTGAGAGAACAATAGA+TGG + chr5.2:62123032-62123051 MS.gene99721:CDS 35.0%
AGAAGGATGAATCATGATGA+TGG + chr5.2:62122912-62122931 MS.gene99721:CDS 35.0%
ATTGATCTTGCAAGTCAATG+GGG - chr5.2:62123172-62123191 None:intergenic 35.0%
GAAGGATGAATCATGATGAT+GGG + chr5.2:62122913-62122932 MS.gene99721:CDS 35.0%
GACTTGCAAGATCAATCATT+TGG + chr5.2:62123176-62123195 MS.gene99721:CDS 35.0%
GATTGATCTTGCAAGTCAAT+GGG - chr5.2:62123173-62123192 None:intergenic 35.0%
TGATTGATCTTGCAAGTCAA+TGG - chr5.2:62123174-62123193 None:intergenic 35.0%
TTCCTCTTCATTGAAGAAGT+TGG - chr5.2:62123104-62123123 None:intergenic 35.0%
!! TCACTTTTACCTTCAGAGAT+TGG + chr5.2:62123146-62123165 MS.gene99721:CDS 35.0%
AGCCAACTTCTTCAATGAAG+AGG + chr5.2:62123099-62123118 MS.gene99721:CDS 40.0%
ATTGATACTGAGGACTTTGC+AGG - chr5.2:62123230-62123249 None:intergenic 40.0%
TTCGTTCGAAATTGGGTTCG+AGG + chr5.2:62122766-62122785 MS.gene99721:CDS 45.0%
TTGGGTTCGAGGCATGAAAT+AGG + chr5.2:62122777-62122796 MS.gene99721:CDS 45.0%
TCAATGGGGCCAATCTCTGA+AGG - chr5.2:62123158-62123177 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 62122705 62123253 62122705 ID=MS.gene99721
chr5.2 mRNA 62122705 62123253 62122705 ID=MS.gene99721.t1;Parent=MS.gene99721
chr5.2 exon 62122705 62123253 62122705 ID=MS.gene99721.t1.exon1;Parent=MS.gene99721.t1
chr5.2 CDS 62122705 62123253 62122705 ID=cds.MS.gene99721.t1;Parent=MS.gene99721.t1
Gene Sequence

>MS.gene99721

ATGAAGATCAATTTGATTCTATCTCTTGTTGCTGCAATCTTATGTTATTCTCTTATTGTTCTTCGTTCGAAATTGGGTTCGAGGCATGAAATAGGAACTTCAAGAGTGCAAGATGATGTAGCTATTGAAATTAATGGTGTAGATAACGATAGAAATGAATTTCAATCTGATTATAGTAATGGCAGCCAACAAAATCAGTCTTTGATAAGAAGGATGAATCATGATGATGGGTATAATTGGAAAAAGTATGAAGATAAAGTAGCAAAAGGAAGTGAAAATCAAAGAAGCTATTATAAATGTACGTGGCCTAATTGTTTAGTGAAGAAAAAGGTTGAGAGAACAATAGATGGAGAAGTTATTGAGACACTATACAAAGGTACACATAATCATTGGAAGCCAACTTCTTCAATGAAGAGGAATTCATCATCTGAGTATCTTTATTCACTTTTACCTTCAGAGATTGGCCCCATTGACTTGCAAGATCAATCATTTGGAAGTGAACAATTGGATTCTGATGAAGAACCTGCAAAGTCCTCAGTATCAATATGA
Protein sequence

>MS.gene99721.t1

MKINLILSLVAAILCYSLIVLRSKLGSRHEIGTSRVQDDVAIEINGVDNDRNEFQSDYSNGSQQNQSLIRRMNHDDGYNWKKYEDKVAKGSENQRSYYKCTWPNCLVKKKVERTIDGEVIETLYKGTHNHWKPTSSMKRNSSSEYLYSLLPSEIGPIDLQDQSFGSEQLDSDEEPAKSSVSI