AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99728

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 109 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCCATATGATTTCTTTCT+TGG 0.151399 2.4:+32748062 None:intergenic
CCTAGAAGGGTATACTTTAA+TGG 0.184050 2.4:-32745951 MS.gene99728:CDS
TATTTCCAGCAACCTCTTTC+AGG 0.198489 2.4:+32746773 None:intergenic
AAGATAATTATAAGGATTAT+TGG 0.226624 2.4:-32746663 MS.gene99728:CDS
AACACAACAGCTTGGGTTCT+TGG 0.235897 2.4:+32747255 None:intergenic
GGTAGCCTTTGAGCAGAATT+TGG 0.247873 2.4:+32745885 None:intergenic
CCATTAAAGTATACCCTTCT+AGG 0.259971 2.4:+32745951 None:intergenic
AACTGTCTTGTTAGTCCTTC+CGG 0.267626 2.4:+32747825 None:intergenic
TGGCGTGTGCATTGCAATAA+AGG 0.319433 2.4:+32746718 None:intergenic
ATTCTACAATGATTTAATAA+TGG 0.323384 2.4:-32746060 MS.gene99728:CDS
GCATATAAAAGATAATTATA+AGG 0.335357 2.4:-32746671 MS.gene99728:CDS
GTGCATTGCAATAAAGGTTT+TGG 0.357973 2.4:+32746724 None:intergenic
GGGTAACATTACTGAGATTA+GGG 0.358595 2.4:+32746569 None:intergenic
TAATGGAAGCTGGGGCTAAA+GGG 0.368160 2.4:-32746043 MS.gene99728:CDS
GATGACACAGGAATGTTCTA+TGG 0.385600 2.4:-32746089 MS.gene99728:CDS
TGTTGCTTGCGCTCCCATCA+TGG 0.386890 2.4:+32748041 None:intergenic
ATGGACATCAGATGGTTATA+TGG 0.392606 2.4:-32748730 MS.gene99728:intron
ATGAACCCGGGATGGACATT+AGG 0.394711 2.4:-32748096 MS.gene99728:CDS
ATCCCGGGTTCATAATGTTT+CGG 0.395348 2.4:+32748106 None:intergenic
AGGTGGTGTGCTAACATCTT+GGG 0.396771 2.4:-32747899 MS.gene99728:CDS
TGGGTAACATTACTGAGATT+AGG 0.404033 2.4:+32746568 None:intergenic
TTTGATCCATTGGATCCAAC+TGG 0.408485 2.4:-32748783 MS.gene99728:CDS
CAAGTAGTTGACTTATTACC+TGG 0.416180 2.4:-32747958 MS.gene99728:CDS
GGGCCAAGAAAGAAATCATA+TGG 0.421488 2.4:-32748065 MS.gene99728:CDS
TGAGCAGAATTTGGCAAGAA+AGG 0.426117 2.4:+32745894 None:intergenic
TAGAATGAATTACACACAAT+GGG 0.426364 2.4:-32746608 MS.gene99728:CDS
ATATGCCAATGCACACGAAC+AGG 0.426620 2.4:+32746688 None:intergenic
ATAATGGAAGCTGGGGCTAA+AGG 0.427586 2.4:-32746044 MS.gene99728:CDS
TCTTGGGGACAGGACCCTTC+AGG 0.429022 2.4:-32747883 MS.gene99728:CDS
AACTTACTTATGGCTTCATA+AGG 0.429069 2.4:+32746514 None:intergenic
GGCAAGAAAGGGTATGAATC+AGG 0.435645 2.4:+32745906 None:intergenic
TGTAGAGGCATTTGATCCAT+TGG 0.442199 2.4:-32748793 None:intergenic
AATGATTTAATAATGGAAGC+TGG 0.446216 2.4:-32746053 MS.gene99728:CDS
TCTGTAAGAATAACAGTTGA+AGG 0.452263 2.4:+32747733 None:intergenic
AAGATCCTGAAAGAGGTTGC+TGG 0.460769 2.4:-32746778 MS.gene99728:CDS
AAATTGCTTGGACCGGGAGC+AGG 0.463331 2.4:-32747784 MS.gene99728:CDS
GAGCAGAATTTGGCAAGAAA+GGG 0.471958 2.4:+32745895 None:intergenic
AGAAATCATATGGGCCATGA+TGG 0.480849 2.4:-32748055 MS.gene99728:CDS
ATGATTTAATAATGGAAGCT+GGG 0.480905 2.4:-32746052 MS.gene99728:CDS
ATAAAGAAACACAACAGCTT+GGG 0.481011 2.4:+32747248 None:intergenic
GTCCATCCTAATGTCCATCC+CGG 0.482281 2.4:+32748090 None:intergenic
TTTGTCCTGTTCGTGTGCAT+TGG 0.488987 2.4:-32746693 MS.gene99728:CDS
TGCAATCTCGGCATTTCAGA+TGG 0.490026 2.4:-32747860 MS.gene99728:CDS
GGACTTCTCTTGCAACTATG+AGG 0.491344 2.4:+32747982 None:intergenic
ATAAGTACATGTCACATTCC+AGG 0.497470 2.4:+32747294 None:intergenic
GATAAAGAAACACAACAGCT+TGG 0.498328 2.4:+32747247 None:intergenic
AGCAGGATATTCATGTGGTC+CGG 0.500729 2.4:-32747767 MS.gene99728:CDS
ACATTCCCAGTTGGATCCAA+TGG 0.502942 2.4:+32748777 None:intergenic
CTGGGAATGTGACAATAAGA+TGG 0.503167 2.4:-32748764 MS.gene99728:CDS
GGACCCTTCAGGTGCAATCT+CGG 0.506260 2.4:-32747872 MS.gene99728:CDS
CAAGACAGTTAAACTACCTC+AGG 0.509450 2.4:-32747812 MS.gene99728:CDS
CAGATGGGTGTAGGACTCTC+CGG 0.515620 2.4:-32747844 MS.gene99728:CDS
ATCTGAGGTGCTTATGAAGA+AGG 0.516290 2.4:-32746012 MS.gene99728:CDS
AAGGTGGTGTGCTAACATCT+TGG 0.517435 2.4:-32747900 MS.gene99728:CDS
GTCATCATTACAACACTTGT+AGG 0.519462 2.4:+32745864 None:intergenic
CATTTACACTAAAGCAAGGA+TGG 0.523081 2.4:-32745980 MS.gene99728:CDS
ACAACAGCTTGGGTTCTTGG+AGG 0.528958 2.4:+32747258 None:intergenic
CTTATTGTCACATTCCCAGT+TGG 0.532085 2.4:+32748768 None:intergenic
TGTATTCAAAACTATAGACT+TGG 0.537143 2.4:+32746548 None:intergenic
GCTTGTCCAACTTGTGCTTG+TGG 0.548007 2.4:-32747199 MS.gene99728:CDS
GGTAATAAGTCAACTACTTG+AGG 0.550335 2.4:+32747961 None:intergenic
TCTTGCCAAATTCTGCTCAA+AGG 0.554568 2.4:-32745890 MS.gene99728:CDS
AAATGATGCAATAATCACAT+AGG 0.564180 2.4:+32745839 None:intergenic
TGCATATTGTAAGATGCTCC+AGG 0.567353 2.4:+32747940 None:intergenic
GAAATCATATGGGCCATGAT+GGG 0.570678 2.4:-32748054 MS.gene99728:CDS
TGTGCTAACATCTTGGGGAC+AGG 0.571959 2.4:-32747893 MS.gene99728:CDS
TACACAAACTGTTGCAAAGG+TGG 0.573020 2.4:-32747916 MS.gene99728:CDS
ATGCCGAGATTGCACCTGAA+GGG 0.577152 2.4:+32747869 None:intergenic
TTGCTGGAAATAACAAGACT+AGG 0.578763 2.4:-32746762 MS.gene99728:CDS
CCACATGAATATCCTGCTCC+CGG 0.579683 2.4:+32747772 None:intergenic
GTTGAAGGAACTCTCTTAGC+CGG 0.580085 2.4:+32747748 None:intergenic
GGAGCGCAAGCAACAGAACA+AGG 0.580360 2.4:-32748033 MS.gene99728:CDS
CAATACACAAACTGTTGCAA+AGG 0.581528 2.4:-32747919 MS.gene99728:CDS
TCGGCATTTCAGATGGGTGT+AGG 0.583929 2.4:-32747853 MS.gene99728:CDS
TGATTTAATAATGGAAGCTG+GGG 0.584574 2.4:-32746051 MS.gene99728:CDS
TGACAACCACAAGCACAAGT+TGG 0.585659 2.4:+32747193 None:intergenic
TGGGAATGTGACAATAAGAT+GGG 0.590389 2.4:-32748763 MS.gene99728:CDS
ATGGCTTCATAAGGAAGAAG+TGG 0.591100 2.4:+32746523 None:intergenic
TCCATCCTAATGTCCATCCC+GGG 0.595187 2.4:+32748091 None:intergenic
AGATTCAAAGATCCTGAAAG+AGG 0.596172 2.4:-32746785 MS.gene99728:CDS
AAGTTGGACAAGCTGTGACT+TGG 0.597255 2.4:+32747209 None:intergenic
AATGCCGAGATTGCACCTGA+AGG 0.597959 2.4:+32747868 None:intergenic
AAAGGGTATGAATCAGGAGG+TGG 0.602047 2.4:+32745912 None:intergenic
GGCCAAGAAAGAAATCATAT+GGG 0.602816 2.4:-32748064 MS.gene99728:CDS
ATTATGTCATGGACATCAGA+TGG 0.603721 2.4:-32748738 MS.gene99728:CDS
GATGGACATTAGGATGGACA+TGG 0.605342 2.4:-32748086 MS.gene99728:CDS
GTATTCAAAACTATAGACTT+GGG 0.605359 2.4:+32746549 None:intergenic
AACACATTTACACTAAAGCA+AGG 0.605862 2.4:-32745984 MS.gene99728:CDS
ATACCGAAACATTATGAACC+CGG 0.606089 2.4:-32748109 MS.gene99728:CDS
GCAATCTCGGCATTTCAGAT+GGG 0.606491 2.4:-32747859 MS.gene99728:CDS
ACCCGGGATGGACATTAGGA+TGG 0.607366 2.4:-32748092 MS.gene99728:CDS
TACCGAAACATTATGAACCC+GGG 0.608883 2.4:-32748108 MS.gene99728:CDS
ATTTACACTAAAGCAAGGAT+GGG 0.609581 2.4:-32745979 MS.gene99728:CDS
TTATTCTTACAGATGATCGT+AGG 0.609804 2.4:-32747723 MS.gene99728:CDS
TTGATCCATTGGATCCAACT+GGG 0.610985 2.4:-32748782 MS.gene99728:CDS
GAAACATTATGAACCCGGGA+TGG 0.613102 2.4:-32748104 MS.gene99728:CDS
TAAGATGGGATATTATGTCA+TGG 0.615769 2.4:-32748749 MS.gene99728:CDS
GGTGGTGTGCTAACATCTTG+GGG 0.624710 2.4:-32747898 MS.gene99728:CDS
ATAGAATGAATTACACACAA+TGG 0.628977 2.4:-32746609 MS.gene99728:CDS
TAAAGGGAATGTGCAATCTG+AGG 0.631122 2.4:-32746027 MS.gene99728:CDS
CCAAGCAATTTAAAATCCTG+AGG 0.636131 2.4:+32747796 None:intergenic
TGGGTGTAGGACTCTCCGGA+AGG 0.662739 2.4:-32747840 MS.gene99728:CDS
AATGCACACGAACAGGACAA+AGG 0.669891 2.4:+32746695 None:intergenic
CCGGGAGCAGGATATTCATG+TGG 0.685860 2.4:-32747772 MS.gene99728:CDS
GCACACGAACAGGACAAAGG+TGG 0.685930 2.4:+32746698 None:intergenic
AAGAAAGGGTATGAATCAGG+AGG 0.687329 2.4:+32745909 None:intergenic
ATGGACATTAGGATGGACAT+GGG 0.698791 2.4:-32748085 MS.gene99728:CDS
AGAATGAATTACACACAATG+GGG 0.725776 2.4:-32746607 MS.gene99728:CDS
ATTGTGTATGCAGATGACAC+AGG 0.753079 2.4:-32746101 MS.gene99728:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATGTTATAACTTATA+AGG + chr2.4:32747760-32747779 None:intergenic 10.0%
!! TTATAAGTTATAACATTATT+TGG - chr2.4:32747759-32747778 MS.gene99728:CDS 10.0%
!!! TTTTGATAATTTTATTCTTT+TGG + chr2.4:32746038-32746057 None:intergenic 10.0%
!! AAGATAATTATAAGGATTAT+TGG - chr2.4:32747949-32747968 MS.gene99728:CDS 15.0%
!! ATTCTACAATGATTTAATAA+TGG - chr2.4:32748552-32748571 MS.gene99728:intron 15.0%
!! GCATATAAAAGATAATTATA+AGG - chr2.4:32747941-32747960 MS.gene99728:CDS 15.0%
!! ATAGGGATATTATATGTATT+TGG + chr2.4:32748436-32748455 None:intergenic 20.0%
!! ATATTTGAAGAACTTACTTA+TGG + chr2.4:32748111-32748130 None:intergenic 20.0%
!! TAATAGTACATTTGTATTCA+AGG + chr2.4:32747258-32747277 None:intergenic 20.0%
!! TATTAGATAATACTATTTCC+AGG + chr2.4:32747180-32747199 None:intergenic 20.0%
!! TCAAGTAAACAAAATATAGA+CGG + chr2.4:32748140-32748159 None:intergenic 20.0%
!! TTAATATTCGCATAATAACA+CGG - chr2.4:32746215-32746234 MS.gene99728:intron 20.0%
!!! AAATAAATTGGCTTGAAATT+TGG + chr2.4:32748305-32748324 None:intergenic 20.0%
!!! AAGACTAATAATCATGATTT+TGG + chr2.4:32747650-32747669 None:intergenic 20.0%
!!! CGAAAAACATATTTTCAAAA+TGG - chr2.4:32746301-32746320 MS.gene99728:intron 20.0%
!!! TTATTATGTGTTACTATATC+AGG + chr2.4:32746168-32746187 None:intergenic 20.0%
!!! TTATTGCATCATTTTTCATT+TGG - chr2.4:32748782-32748801 MS.gene99728:CDS 20.0%
! AAATGATGCAATAATCACAT+AGG + chr2.4:32748776-32748795 None:intergenic 25.0%
! ACTATTTGCAATATACTCAA+AGG + chr2.4:32746950-32746969 None:intergenic 25.0%
! AGTAACTGAGATTCATTTAA+AGG + chr2.4:32746332-32746351 None:intergenic 25.0%
! ATAGAATGAATTACACACAA+TGG - chr2.4:32748003-32748022 MS.gene99728:CDS 25.0%
! CTAGAAAAGGATTTGAATAA+TGG - chr2.4:32746126-32746145 MS.gene99728:intron 25.0%
! CTATTTGCAATATACTCAAA+GGG + chr2.4:32746949-32746968 None:intergenic 25.0%
! GAAATTCAGTCAAATACAAT+TGG - chr2.4:32747548-32747567 MS.gene99728:intron 25.0%
! GAGTATATTGCAAATAGTTA+TGG - chr2.4:32746952-32746971 MS.gene99728:intron 25.0%
! GTATTCAAAACTATAGACTT+GGG + chr2.4:32748066-32748085 None:intergenic 25.0%
! GTTGCATCATAATCATTTAT+AGG - chr2.4:32747521-32747540 MS.gene99728:intron 25.0%
! TAATGTGGAAGTAGAAATTA+AGG + chr2.4:32747474-32747493 None:intergenic 25.0%
! TAGAATGAATTACACACAAT+GGG - chr2.4:32748004-32748023 MS.gene99728:CDS 25.0%
! TGTATTCAAAACTATAGACT+TGG + chr2.4:32748067-32748086 None:intergenic 25.0%
! TTAAAAGTTGGCTAATAATG+TGG + chr2.4:32747489-32747508 None:intergenic 25.0%
! TTGGATTGTACGATATTAAT+CGG + chr2.4:32748286-32748305 None:intergenic 25.0%
!! AATAATCATGATTTTGGACA+CGG + chr2.4:32747644-32747663 None:intergenic 25.0%
!! AATGATTTAATAATGGAAGC+TGG - chr2.4:32748559-32748578 MS.gene99728:intron 25.0%
!! ATGATTTAATAATGGAAGCT+GGG - chr2.4:32748560-32748579 MS.gene99728:intron 25.0%
!! GATACTATGATGTCAAATTT+TGG - chr2.4:32746448-32746467 MS.gene99728:intron 25.0%
!!! CACAATATAAAAGGTTTTAG+AGG + chr2.4:32747704-32747723 None:intergenic 25.0%
!!! CATCATTTTTGGAAACATTT+TGG + chr2.4:32745992-32746011 None:intergenic 25.0%
!!! CATTTTTCATTTGGTTTTGA+AGG - chr2.4:32748791-32748810 MS.gene99728:CDS 25.0%
!!! CTCTAAAACCTTTTATATTG+TGG - chr2.4:32747702-32747721 MS.gene99728:intron 25.0%
!!! GATTATTAGTCTTAACCATA+TGG - chr2.4:32747657-32747676 MS.gene99728:intron 25.0%
!!! GTTTTTTTAACTTTTGTGCA+TGG - chr2.4:32748484-32748503 MS.gene99728:intron 25.0%
AAAATACCAAGCTTGAAATC+TGG - chr2.4:32748338-32748357 MS.gene99728:intron 30.0%
AACACATTTACACTAAAGCA+AGG - chr2.4:32748628-32748647 MS.gene99728:intron 30.0%
AACTTACTTATGGCTTCATA+AGG + chr2.4:32748101-32748120 None:intergenic 30.0%
ACTGCATAGGAAAAAAATAC+AGG + chr2.4:32747296-32747315 None:intergenic 30.0%
AGAATGAATTACACACAATG+GGG - chr2.4:32748005-32748024 MS.gene99728:CDS 30.0%
ATAAAGAAACACAACAGCTT+GGG + chr2.4:32747367-32747386 None:intergenic 30.0%
ATGTGACTCTATGCATTATA+GGG + chr2.4:32748453-32748472 None:intergenic 30.0%
ATTTACACTAAAGCAAGGAT+GGG - chr2.4:32748633-32748652 MS.gene99728:intron 30.0%
ATTTGTCATTATGCAAATGC+AGG - chr2.4:32747802-32747821 MS.gene99728:CDS 30.0%
CTACATTTGACCATAATTCT+CGG + chr2.4:32747027-32747046 None:intergenic 30.0%
GAAAAGGATTTGAATAATGG+TGG - chr2.4:32746129-32746148 MS.gene99728:intron 30.0%
TAAGATGGGATATTATGTCA+TGG - chr2.4:32745863-32745882 MS.gene99728:CDS 30.0%
TCCAAAAATGATGAAATCAC+TGG - chr2.4:32745999-32746018 MS.gene99728:CDS 30.0%
TCTGTAAGAATAACAGTTGA+AGG + chr2.4:32746882-32746901 None:intergenic 30.0%
TGACGTCTCTAATACAAATT+CGG - chr2.4:32748210-32748229 MS.gene99728:intron 30.0%
TTATTCTTACAGATGATCGT+AGG - chr2.4:32746889-32746908 MS.gene99728:intron 30.0%
TTTAATACTGCCGAGAATTA+TGG - chr2.4:32747014-32747033 MS.gene99728:intron 30.0%
! ACCAGTGATTTCATCATTTT+TGG + chr2.4:32746003-32746022 None:intergenic 30.0%
! CTGATCATGCTATTCTTTTA+AGG - chr2.4:32745926-32745945 MS.gene99728:CDS 30.0%
! GTTGTCTTATTAAGATCAGA+CGG + chr2.4:32748365-32748384 None:intergenic 30.0%
! TGATTTAATAATGGAAGCTG+GGG - chr2.4:32748561-32748580 MS.gene99728:intron 30.0%
AATGAATCTCAGTTACTTCG+AGG - chr2.4:32746335-32746354 MS.gene99728:intron 35.0%
AGATTCAAAGATCCTGAAAG+AGG - chr2.4:32747827-32747846 MS.gene99728:CDS 35.0%
AGGATCTGCAATTACAATCA+AGG + chr2.4:32747112-32747131 None:intergenic 35.0%
ATAAGTACATGTCACATTCC+AGG + chr2.4:32747321-32747340 None:intergenic 35.0%
ATACCGAAACATTATGAACC+CGG - chr2.4:32746503-32746522 MS.gene99728:intron 35.0%
ATGAATCTCAGTTACTTCGA+GGG - chr2.4:32746336-32746355 MS.gene99728:intron 35.0%
ATGGACATCAGATGGTTATA+TGG - chr2.4:32745882-32745901 MS.gene99728:CDS 35.0%
ATTATGTCATGGACATCAGA+TGG - chr2.4:32745874-32745893 MS.gene99728:CDS 35.0%
CAATACACAAACTGTTGCAA+AGG - chr2.4:32746693-32746712 MS.gene99728:CDS 35.0%
CATGTGACTCTATGCATTAT+AGG + chr2.4:32748454-32748473 None:intergenic 35.0%
CATTGCAATCAACATGTTTG+TGG - chr2.4:32748256-32748275 MS.gene99728:intron 35.0%
CATTTACACTAAAGCAAGGA+TGG - chr2.4:32748632-32748651 MS.gene99728:intron 35.0%
CCATTAAAGTATACCCTTCT+AGG + chr2.4:32748664-32748683 None:intergenic 35.0%
GATAAAGAAACACAACAGCT+TGG + chr2.4:32747368-32747387 None:intergenic 35.0%
GGCCAAGAAAGAAATCATAT+GGG - chr2.4:32746548-32746567 MS.gene99728:CDS 35.0%
GGGTAACATTACTGAGATTA+GGG + chr2.4:32748046-32748065 None:intergenic 35.0%
GGTAATAAGTCAACTACTTG+AGG + chr2.4:32746654-32746673 None:intergenic 35.0%
GTCATCATTACAACACTTGT+AGG + chr2.4:32748751-32748770 None:intergenic 35.0%
GTGTAACACACATAGATGTT+TGG - chr2.4:32746237-32746256 MS.gene99728:intron 35.0%
TAGATTCGAGAAGCTAGAAA+AGG - chr2.4:32746113-32746132 MS.gene99728:intron 35.0%
TAGCATGATCAGACAAAGTT+TGG + chr2.4:32745918-32745937 None:intergenic 35.0%
TGGGAATGTGACAATAAGAT+GGG - chr2.4:32745849-32745868 MS.gene99728:CDS 35.0%
TGGGTAACATTACTGAGATT+AGG + chr2.4:32748047-32748066 None:intergenic 35.0%
TTAATCTCCACAATTACACG+TGG - chr2.4:32746360-32746379 MS.gene99728:intron 35.0%
TTGCTGGAAATAACAAGACT+AGG - chr2.4:32747850-32747869 MS.gene99728:CDS 35.0%
! AACCATCAAGGTTTTCGAAA+AGG + chr2.4:32747132-32747151 None:intergenic 35.0%
! ATCCTTTTCGAAAACCTTGA+TGG - chr2.4:32747127-32747146 MS.gene99728:intron 35.0%
! CAAGTAGTTGACTTATTACC+TGG - chr2.4:32746654-32746673 MS.gene99728:CDS 35.0%
! CCAAGCAATTTAAAATCCTG+AGG + chr2.4:32746819-32746838 None:intergenic 35.0%
! CCTAGAAGGGTATACTTTAA+TGG - chr2.4:32748661-32748680 MS.gene99728:intron 35.0%
! CCTCAGGATTTTAAATTGCT+TGG - chr2.4:32746816-32746835 MS.gene99728:intron 35.0%
! GATTTTAAATTGCTTGGACC+GGG - chr2.4:32746822-32746841 MS.gene99728:intron 35.0%
! GGATTTTAAATTGCTTGGAC+CGG - chr2.4:32746821-32746840 MS.gene99728:intron 35.0%
! GTGCATTGCAATAAAGGTTT+TGG + chr2.4:32747891-32747910 None:intergenic 35.0%
!! ATATGTTTTTCGATACGCAC+TGG + chr2.4:32746293-32746312 None:intergenic 35.0%
!! CTATGATGTCAAATTTTGGC+AGG - chr2.4:32746452-32746471 MS.gene99728:intron 35.0%
AACTGCGGCCACAATATAAA+AGG + chr2.4:32747713-32747732 None:intergenic 40.0%
AATTGCACGCATTCATACAG+TGG + chr2.4:32748398-32748417 None:intergenic 40.0%
ACACGAACTCCTATGGTTAA+GGG + chr2.4:32746265-32746284 None:intergenic 40.0%
AGAAATCATATGGGCCATGA+TGG - chr2.4:32746557-32746576 MS.gene99728:CDS 40.0%
ATCCCGGGTTCATAATGTTT+CGG + chr2.4:32746509-32746528 None:intergenic 40.0%
ATGGACATTAGGATGGACAT+GGG - chr2.4:32746527-32746546 MS.gene99728:CDS 40.0%
ATGGCTTCATAAGGAAGAAG+TGG + chr2.4:32748092-32748111 None:intergenic 40.0%
ATTGTGTATGCAGATGACAC+AGG - chr2.4:32748511-32748530 MS.gene99728:intron 40.0%
CAAGACAGTTAAACTACCTC+AGG - chr2.4:32746800-32746819 MS.gene99728:intron 40.0%
CTATCATCCACGTGTAATTG+TGG + chr2.4:32746370-32746389 None:intergenic 40.0%
CTCGACAGCAGTTTAAAAGT+TGG + chr2.4:32747501-32747520 None:intergenic 40.0%
CTGGGAATGTGACAATAAGA+TGG - chr2.4:32745848-32745867 MS.gene99728:CDS 40.0%
CTTATTGTCACATTCCCAGT+TGG + chr2.4:32745847-32745866 None:intergenic 40.0%
GAAATCATATGGGCCATGAT+GGG - chr2.4:32746558-32746577 MS.gene99728:CDS 40.0%
GAGCAGAATTTGGCAAGAAA+GGG + chr2.4:32748720-32748739 None:intergenic 40.0%
GATGACACAGGAATGTTCTA+TGG - chr2.4:32748523-32748542 MS.gene99728:intron 40.0%
GGCCCATATGATTTCTTTCT+TGG + chr2.4:32746553-32746572 None:intergenic 40.0%
GGGCCAAGAAAGAAATCATA+TGG - chr2.4:32746547-32746566 MS.gene99728:CDS 40.0%
TAAAGGGAATGTGCAATCTG+AGG - chr2.4:32748585-32748604 MS.gene99728:intron 40.0%
TAACTGCTATTGCAGCCATA+TGG + chr2.4:32747675-32747694 None:intergenic 40.0%
TACACAAACTGTTGCAAAGG+TGG - chr2.4:32746696-32746715 MS.gene99728:CDS 40.0%
TACCGAAACATTATGAACCC+GGG - chr2.4:32746504-32746523 MS.gene99728:intron 40.0%
TATTTCCAGCAACCTCTTTC+AGG + chr2.4:32747842-32747861 None:intergenic 40.0%
TCTTGCCAAATTCTGCTCAA+AGG - chr2.4:32748722-32748741 MS.gene99728:intron 40.0%
TGAATCTCAGTTACTTCGAG+GGG - chr2.4:32746337-32746356 MS.gene99728:intron 40.0%
TGAGCAGAATTTGGCAAGAA+AGG + chr2.4:32748721-32748740 None:intergenic 40.0%
TGCATATTGTAAGATGCTCC+AGG + chr2.4:32746675-32746694 None:intergenic 40.0%
TGTTTGGATCCCTTAACCAT+AGG - chr2.4:32746253-32746272 MS.gene99728:intron 40.0%
TTCATACAGTGGCACATATG+TGG + chr2.4:32748387-32748406 None:intergenic 40.0%
TTGATCCATTGGATCCAACT+GGG - chr2.4:32745830-32745849 MS.gene99728:CDS 40.0%
TTTGATCCATTGGATCCAAC+TGG - chr2.4:32745829-32745848 MS.gene99728:CDS 40.0%
! AACTGTCTTGTTAGTCCTTC+CGG + chr2.4:32746790-32746809 None:intergenic 40.0%
! AAGAAAGGGTATGAATCAGG+AGG + chr2.4:32748706-32748725 None:intergenic 40.0%
! ATCTGAGGTGCTTATGAAGA+AGG - chr2.4:32748600-32748619 MS.gene99728:intron 40.0%
! CAACTTTTAAACTGCTGTCG+AGG - chr2.4:32747499-32747518 MS.gene99728:intron 40.0%
!! ACGCTTTAAAGAAACTGTCC+TGG - chr2.4:32747159-32747178 MS.gene99728:intron 40.0%
!! AGCGTTGATTGTAACCATCA+AGG + chr2.4:32747144-32747163 None:intergenic 40.0%
!!! TTTTTTCCTATGCAGTGTCC+TGG - chr2.4:32747300-32747319 MS.gene99728:CDS 40.0%
AACACAACAGCTTGGGTTCT+TGG + chr2.4:32747360-32747379 None:intergenic 45.0%
AACCATAGGAGTTCGTGTCA+TGG - chr2.4:32746267-32746286 MS.gene99728:intron 45.0%
AAGATCCTGAAAGAGGTTGC+TGG - chr2.4:32747834-32747853 MS.gene99728:CDS 45.0%
AAGGTGGTGTGCTAACATCT+TGG - chr2.4:32746712-32746731 MS.gene99728:CDS 45.0%
AAGTTGGACAAGCTGTGACT+TGG + chr2.4:32747406-32747425 None:intergenic 45.0%
AATGCACACGAACAGGACAA+AGG + chr2.4:32747920-32747939 None:intergenic 45.0%
ACATTCCCAGTTGGATCCAA+TGG + chr2.4:32745838-32745857 None:intergenic 45.0%
AGCAGGATATTCATGTGGTC+CGG - chr2.4:32746845-32746864 MS.gene99728:intron 45.0%
AGGTGGTGTGCTAACATCTT+GGG - chr2.4:32746713-32746732 MS.gene99728:CDS 45.0%
ATATGCCAATGCACACGAAC+AGG + chr2.4:32747927-32747946 None:intergenic 45.0%
GAAACATTATGAACCCGGGA+TGG - chr2.4:32746508-32746527 MS.gene99728:intron 45.0%
GACACGAACTCCTATGGTTA+AGG + chr2.4:32746266-32746285 None:intergenic 45.0%
GATGGACATTAGGATGGACA+TGG - chr2.4:32746526-32746545 MS.gene99728:CDS 45.0%
GCAATCTCGGCATTTCAGAT+GGG - chr2.4:32746753-32746772 MS.gene99728:CDS 45.0%
GGACTTCTCTTGCAACTATG+AGG + chr2.4:32746633-32746652 None:intergenic 45.0%
GGTAGCCTTTGAGCAGAATT+TGG + chr2.4:32748730-32748749 None:intergenic 45.0%
GTTGAAGGAACTCTCTTAGC+CGG + chr2.4:32746867-32746886 None:intergenic 45.0%
TAATGGAAGCTGGGGCTAAA+GGG - chr2.4:32748569-32748588 MS.gene99728:intron 45.0%
TGACAACCACAAGCACAAGT+TGG + chr2.4:32747422-32747441 None:intergenic 45.0%
TGCAATCTCGGCATTTCAGA+TGG - chr2.4:32746752-32746771 MS.gene99728:CDS 45.0%
TGGCGTGTGCATTGCAATAA+AGG + chr2.4:32747897-32747916 None:intergenic 45.0%
TTTGTCCTGTTCGTGTGCAT+TGG - chr2.4:32747919-32747938 MS.gene99728:CDS 45.0%
! AAAGGGTATGAATCAGGAGG+TGG + chr2.4:32748703-32748722 None:intergenic 45.0%
! ATAATGGAAGCTGGGGCTAA+AGG - chr2.4:32748568-32748587 MS.gene99728:intron 45.0%
! GGCAAGAAAGGGTATGAATC+AGG + chr2.4:32748709-32748728 None:intergenic 45.0%
!! AAGGATGGGCTTTTCCTAGA+AGG - chr2.4:32748647-32748666 MS.gene99728:intron 45.0%
!! AGGATGGGCTTTTCCTAGAA+GGG - chr2.4:32748648-32748667 MS.gene99728:intron 45.0%
!! GATTAAAAAAATAAATAAAT+TGG + chr2.4:32748317-32748336 None:intergenic 5.0%
AATGCCGAGATTGCACCTGA+AGG + chr2.4:32746747-32746766 None:intergenic 50.0%
ACAACAGCTTGGGTTCTTGG+AGG + chr2.4:32747357-32747376 None:intergenic 50.0%
ATGAACCCGGGATGGACATT+AGG - chr2.4:32746516-32746535 MS.gene99728:intron 50.0%
ATGCCGAGATTGCACCTGAA+GGG + chr2.4:32746746-32746765 None:intergenic 50.0%
CACAAAGCGACGATCACTAC+AGG - chr2.4:32747226-32747245 MS.gene99728:CDS 50.0%
CACATTCCAGGACACTGCAT+AGG + chr2.4:32747309-32747328 None:intergenic 50.0%
CCACATGAATATCCTGCTCC+CGG + chr2.4:32746843-32746862 None:intergenic 50.0%
CTGCAATACGACTGAGACTG+AGG + chr2.4:32747059-32747078 None:intergenic 50.0%
GACGGTCCAGATTTCAAGCT+TGG + chr2.4:32748347-32748366 None:intergenic 50.0%
GCTTGTCCAACTTGTGCTTG+TGG - chr2.4:32747413-32747432 MS.gene99728:intron 50.0%
GGTGGTGTGCTAACATCTTG+GGG - chr2.4:32746714-32746733 MS.gene99728:CDS 50.0%
GTCCATCCTAATGTCCATCC+CGG + chr2.4:32746525-32746544 None:intergenic 50.0%
TCCATCCTAATGTCCATCCC+GGG + chr2.4:32746524-32746543 None:intergenic 50.0%
TCGGCATTTCAGATGGGTGT+AGG - chr2.4:32746759-32746778 MS.gene99728:CDS 50.0%
TGTGCTAACATCTTGGGGAC+AGG - chr2.4:32746719-32746738 MS.gene99728:CDS 50.0%
AAATTGCTTGGACCGGGAGC+AGG - chr2.4:32746828-32746847 MS.gene99728:intron 55.0%
ACCCGGGATGGACATTAGGA+TGG - chr2.4:32746520-32746539 MS.gene99728:intron 55.0%
CAGATGGGTGTAGGACTCTC+CGG - chr2.4:32746768-32746787 MS.gene99728:CDS 55.0%
CCGGGAGCAGGATATTCATG+TGG - chr2.4:32746840-32746859 MS.gene99728:intron 55.0%
GCACACGAACAGGACAAAGG+TGG + chr2.4:32747917-32747936 None:intergenic 55.0%
GGACCCTTCAGGTGCAATCT+CGG - chr2.4:32746740-32746759 MS.gene99728:CDS 55.0%
GGAGCGCAAGCAACAGAACA+AGG - chr2.4:32746579-32746598 MS.gene99728:CDS 55.0%
GGCCATGACACGAACTCCTA+TGG + chr2.4:32746272-32746291 None:intergenic 55.0%
! TGTTGCTTGCGCTCCCATCA+TGG + chr2.4:32746574-32746593 None:intergenic 55.0%
GCGTCCTGTGACTGCAACTG+CGG + chr2.4:32747728-32747747 None:intergenic 60.0%
TCTTGGGGACAGGACCCTTC+AGG - chr2.4:32746729-32746748 MS.gene99728:CDS 60.0%
TGGGTGTAGGACTCTCCGGA+AGG - chr2.4:32746772-32746791 MS.gene99728:CDS 60.0%
GTGGCCGCAGTTGCAGTCAC+AGG - chr2.4:32747721-32747740 MS.gene99728:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32745824 32748810 32745824 ID=MS.gene99728
chr2.4 mRNA 32745824 32748810 32745824 ID=MS.gene99728.t1;Parent=MS.gene99728
chr2.4 exon 32748731 32748810 32748731 ID=MS.gene99728.t1.exon1;Parent=MS.gene99728.t1
chr2.4 CDS 32748731 32748810 32748731 ID=cds.MS.gene99728.t1;Parent=MS.gene99728.t1
chr2.4 exon 32747704 32748160 32747704 ID=MS.gene99728.t1.exon2;Parent=MS.gene99728.t1
chr2.4 CDS 32747704 32748160 32747704 ID=cds.MS.gene99728.t1;Parent=MS.gene99728.t1
chr2.4 exon 32747172 32747319 32747172 ID=MS.gene99728.t1.exon3;Parent=MS.gene99728.t1
chr2.4 CDS 32747172 32747319 32747172 ID=cds.MS.gene99728.t1;Parent=MS.gene99728.t1
chr2.4 exon 32746521 32746810 32746521 ID=MS.gene99728.t1.exon4;Parent=MS.gene99728.t1
chr2.4 CDS 32746521 32746810 32746521 ID=cds.MS.gene99728.t1;Parent=MS.gene99728.t1
chr2.4 exon 32745824 32746110 32745824 ID=MS.gene99728.t1.exon5;Parent=MS.gene99728.t1
chr2.4 CDS 32745824 32746110 32745824 ID=cds.MS.gene99728.t1;Parent=MS.gene99728.t1
Gene Sequence

>MS.gene99728

AGGCATTTGATCCATTGGATCCAACTGGGAATGTGACAATAAGATGGGATATTATGTCATGGACATCAGATGGTTATATGGTAAGTAAATTCCAAACTTTGTCTGATCATGCTATTCTTTTAAGGAAAAGTTAACTACAAATGTAATCATTATAAGTTGTGCATACCAAAATGTTTCCAAAAATGATGAAATCACTGGTCTTTTGTGATTACCAAAAGAATAAAATTATCAAAATTCAAATATTATGAACTTATAATATATAATCTGATGCTGATGTCGAACTAAGAAATAGATTCGAGAAGCTAGAAAAGGATTTGAATAATGGTGGATTAATTGATACTCCTGATATAGTAACACATAATAATACTCTATATGATAGAAGTGTTTCATCTTAATATTCGCATAATAACACGGTGTAACACACATAGATGTTTGGATCCCTTAACCATAGGAGTTCGTGTCATGGCCAGTGCGTATCGAAAAACATATTTTCAAAATGGTGAATCCTTTAAATGAATCTCAGTTACTTCGAGGGGTTAATCTCCACAATTACACGTGGATGATAGCTTAATTTACACACAAAAATTCGCTAATTAATGCTCTAGCTATCTTCAAACTAATCAAGATACTATGATGTCAAATTTTGGCAGGCAACTGTAACTTTGTTTAACTTTCAACTATACCGAAACATTATGAACCCGGGATGGACATTAGGATGGACATGGGCCAAGAAAGAAATCATATGGGCCATGATGGGAGCGCAAGCAACAGAACAAGGAGATTGTTCAAAGTTCAAGTTAAAGATTCCTCATAGTTGCAAGAGAAGTCCTCAAGTAGTTGACTTATTACCTGGAGCATCTTACAATATGCAATACACAAACTGTTGCAAAGGTGGTGTGCTAACATCTTGGGGACAGGACCCTTCAGGTGCAATCTCGGCATTTCAGATGGGTGTAGGACTCTCCGGAAGGACTAACAAGACAGTTAAACTACCTCAGGATTTTAAATTGCTTGGACCGGGAGCAGGATATTCATGTGGTCCGGCTAAGAGAGTTCCTTCAACTGTTATTCTTACAGATGATCGTAGGCGAAAAGCTCAAGCTTTGAGTATGCATACTAATTCCCTTTGAGTATATTGCAAATAGTTATGGTTTACAATCACGATTTTAAATTGAATCTGCATCGTAATTTTTAATACTGCCGAGAATTATGGTCAAATGTAGCTGATGCAACCTCAGTCTCAGTCGTATTGCAGTCATTGTGACATCAAAACATGATGTCGCGACCTTGATTGTAATTGCAGATCCTTTTCGAAAACCTTGATGGTTACAATCAACGCTTTAAAGAAACTGTCCTGGAAATAGTATTATCTAATATTCGATAACAAAGTTTGTGATACAAACACAAAGCGACGATCACTACAGGTTAAAACCTTGAATACAAATGTACTATTAATATTATCTAATGTTCCTGTATTTTTTTCCTATGCAGTGTCCTGGAATGTGACATGTACTTATTCACAATTTCTTGCCTCCAAGAACCCAAGCTGTTGTGTTTCTTTATCAACATTCTACAATGACCAAGTCACAGCTTGTCCAACTTGTGCTTGTGGTTGTCAGAATAATGCTACTTGCGTAACGTAAGACGCCTTAATTTCTACTTCCACATTATTAGCCAACTTTTAAACTGCTGTCGAGGTTGCATCATAATCATTTATAGGACAAGAAATTCAGTCAAATACAATTGGTTGATGCAACTTCAATTGCAGTTGTAGAGACATCGTTTTGTGATGTCGCAGCGTAGATTACAAAAGCTTTCCGTGTCCAAAATCATGATTATTAGTCTTAACCATATGGCTGCAATAGCAGTTATAGAAACCTCTAAAACCTTTTATATTGTGGCCGCAGTTGCAGTCACAGGACGCAATTTAGAGCCTTATAAGTTATAACATTATTTGGAAATAGTAATGCTAATTTTGATTTGTCATTATGCAAATGCAGGAAAGATTCAAAGATCCTGAAAGAGGTTGCTGGAAATAACAAGACTAGGAAGAGTGATATCGCACCAAAACCTTTATTGCAATGCACACGCCACCTTTGTCCTGTTCGTGTGCATTGGCATATAAAAGATAATTATAAGGATTATTGGCGTGTAAAGATTGCAATCATCAACTTCAAGTATAGAATGAATTACACACAATGGGGTCTTGTTGTGCAACACCCTAATCTCAGTAATGTTACCCAAGTCTATAGTTTTGAATACATGCCACTTCTTCCTTATGAAGCCATAAGTAAGTTCTTCAAATATTAGACACCGTCTATATTTTGTTTACTTGAATTACAAAATGAAATTAACAGAACAAGAAAATGAATTTCTTATCTAACTTTGACGTCTCTAATACAAATTCGGATTGCATGCAGTCACAATTGTTGCATTGCAATCAACATGTTTGTGGTCATCCGATTAATATCGTACAATCCAAATTTCAAGCCAATTTATTTATTTTTTTAATCTAAAATACCAAGCTTGAAATCTGGACCGTCTGATCTTAATAAGACAACCACATATGTGCCACTGTATGAATGCGTGCAATTTCGAGTGCATACAATCCAAATACATATAATATCCCTATAATGCATAGAGTCACATGAAGAGTTATTGTTTTTTTAACTTTTGTGCATGGCTGAATTGTGTATGCAGATGACACAGGAATGTTCTATGGTTTGAAATTCTACAATGATTTAATAATGGAAGCTGGGGCTAAAGGGAATGTGCAATCTGAGGTGCTTATGAAGAAGGACAAGAACACATTTACACTAAAGCAAGGATGGGCTTTTCCTAGAAGGGTATACTTTAATGGTGATGAATGTATGCTTCCACCTCCTGATTCATACCCTTTCTTGCCAAATTCTGCTCAAAGGCTACCTACAAGTGTTGTAATGATGACAGCCTATGTGATTATTGCATCATTTTTCATTTGGTTTTGA
Protein sequence

>MS.gene99728.t1

RHLIHWIQLGMUQUDGILCHGHQMVIWQLULCLTFNYTETLUTRDGHUDGHGPRKKSYGPUWERKQQNKEIVQSSSURFLIVAREVLKULTYYLEHLTICNTQTVAKVVCUHLGDRTLQVQSRHFRWVUDSPEGLTRQLNYLRILNCLDREQDIHVVRLREFLQLLFLQMIVGEKLKLUCPGMUHVLIHNFLPPRTQAVVFLYQHSTMTKSQLVQLVLVVVRIMLLAURKIQRSUKRLLEITRLGRVISHQNLYCNAHATFVLFVCIGIUKIIIRIIGVURLQSSTSSIEUITHNGVLLCNTLISVMLPKSIVLNTCHFFLMKPUMTQECSMVUNSTMIUUWKLGLKGMCNLRCLURRTRTHLHUSKDGLFLEGYTLMVMNVCFHLLIHTLSCQILLKGYLQVLUUUQPMULLHHFSFGF