Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99941.t1 | XP_003614714.1 | 96 | 248 | 10 | 0 | 1 | 248 | 1 | 248 | 2.20E-109 | 405.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99941.t1 | B6K8A0 | 35.9 | 170 | 108 | 1 | 77 | 246 | 115 | 283 | 8.5e-22 | 105.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99941.t1 | G7JXB6 | 96.0 | 248 | 10 | 0 | 1 | 248 | 1 | 248 | 1.6e-109 | 405.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene99941 | MS.gene67483 | PPI |
| MS.gene99941 | MS.gene93333 | PPI |
| MS.gene99941 | MS.gene040757 | PPI |
| MS.gene59503 | MS.gene99941 | PPI |
| MS.gene99941 | MS.gene073734 | PPI |
| MS.gene64804 | MS.gene99941 | PPI |
| MS.gene99941 | MS.gene035486 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99941.t1 | MTR_5g058150 | 96.774 | 248 | 8 | 0 | 1 | 248 | 1 | 248 | 2.21e-177 | 488 |
| MS.gene99941.t1 | MTR_6g075250 | 75.403 | 248 | 58 | 3 | 1 | 247 | 1 | 246 | 7.86e-121 | 344 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99941.t1 | AT1G12650 | 58.794 | 199 | 79 | 2 | 52 | 247 | 48 | 246 | 2.80e-70 | 216 |
| MS.gene99941.t1 | AT1G12650 | 58.794 | 199 | 79 | 2 | 52 | 247 | 48 | 246 | 2.80e-70 | 216 |
| MS.gene99941.t1 | AT1G12650 | 58.794 | 199 | 79 | 2 | 52 | 247 | 48 | 246 | 2.80e-70 | 216 |
| MS.gene99941.t1 | AT1G12650 | 58.794 | 199 | 79 | 2 | 52 | 247 | 48 | 246 | 2.80e-70 | 216 |
Find 38 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTACATTTCTTGAGATAAA+AGG | 0.164847 | 5.3:-46329478 | None:intergenic |
| GAATATTGAGACACAGATTT+TGG | 0.225799 | 5.3:+46329407 | MS.gene99941:CDS |
| TGGGTTTCATCGGTTGAATT+TGG | 0.277771 | 5.3:-46323357 | None:intergenic |
| ACAAGTAATCTCACCTTCTT+AGG | 0.296154 | 5.3:-46324960 | None:intergenic |
| TGGCATAACAGGCTCTTAAA+AGG | 0.329338 | 5.3:+46329205 | MS.gene99941:intron |
| GGGTTTCATCGGTTGAATTT+GGG | 0.335327 | 5.3:-46323356 | None:intergenic |
| TTTCAACAAGCCTTTGCTTC+CGG | 0.346969 | 5.3:-46330938 | None:intergenic |
| TACGATATGGCATGAATCTA+TGG | 0.372533 | 5.3:-46331142 | None:intergenic |
| AGAGAGAGAAGCAGCAAAGC+AGG | 0.383818 | 5.3:+46329446 | MS.gene99941:CDS |
| TTTAACTTCTTATCTTCTGC+AGG | 0.387171 | 5.3:-46323622 | None:intergenic |
| CCACGATTTGAGTCTCTTTG+CGG | 0.402540 | 5.3:+46325422 | MS.gene99941:CDS |
| TTCTTGCTACTAGCTTCCAT+TGG | 0.435250 | 5.3:-46324903 | None:intergenic |
| TTACATTTCTTGAGATAAAA+GGG | 0.436036 | 5.3:-46329477 | None:intergenic |
| TCTATTTCACTTCTACGCTC+TGG | 0.442608 | 5.3:-46329249 | None:intergenic |
| TGCAGAAAGCTCGATCGAAT+GGG | 0.447640 | 5.3:+46323578 | MS.gene99941:CDS |
| CTCCTCGTAGTGGGTTTCAT+CGG | 0.452674 | 5.3:-46323367 | None:intergenic |
| TTCATGCCATATCGTAGATC+TGG | 0.467923 | 5.3:+46331149 | MS.gene99941:CDS |
| ACATCTCTAAAAGCAGGAAC+TGG | 0.493887 | 5.3:-46324927 | None:intergenic |
| GCGGTACATTTGACAATGAT+CGG | 0.498086 | 5.3:+46325441 | MS.gene99941:CDS |
| AAGCCTTTGCTTCCGGATCT+CGG | 0.499116 | 5.3:-46330931 | None:intergenic |
| TGATCTTCCTGCTGAAAGAC+AGG | 0.502469 | 5.3:+46329105 | MS.gene99941:CDS |
| TTGCAGAAAGCTCGATCGAA+TGG | 0.509933 | 5.3:+46323577 | MS.gene99941:CDS |
| CAGCCGAGATCCGGAAGCAA+AGG | 0.510746 | 5.3:+46330928 | MS.gene99941:intron |
| GCTAGCTGATGTAACATTCG+AGG | 0.511133 | 5.3:+46323552 | MS.gene99941:CDS |
| AGTTTCATTCTCCTCGTAGT+GGG | 0.519416 | 5.3:-46323376 | None:intergenic |
| CAGTTTCATTCTCCTCGTAG+TGG | 0.524074 | 5.3:-46323377 | None:intergenic |
| GAGAGAGAAGCAGCAAAGCA+GGG | 0.548569 | 5.3:+46329447 | MS.gene99941:CDS |
| TGAATGACATCTCTAAAAGC+AGG | 0.560062 | 5.3:-46324933 | None:intergenic |
| CTACCTACCTGTCTTTCAGC+AGG | 0.562589 | 5.3:-46329112 | None:intergenic |
| AAATAGAAGAGCACATAGCA+TGG | 0.575579 | 5.3:+46329265 | MS.gene99941:CDS |
| TGTCATTCAAGCTCCTAAGA+AGG | 0.575683 | 5.3:+46324947 | MS.gene99941:CDS |
| GAGGAAGAGAAATGCTGCAA+AGG | 0.582512 | 5.3:+46331118 | MS.gene99941:CDS |
| GTTGCTTACATCTTCTGATG+AGG | 0.595546 | 5.3:-46323421 | None:intergenic |
| AATAGAAGAGCACATAGCAT+GGG | 0.631081 | 5.3:+46329266 | MS.gene99941:CDS |
| CCGCAAAGAGACTCAAATCG+TGG | 0.650639 | 5.3:-46325422 | None:intergenic |
| GCAGAAAGCTCGATCGAATG+GGG | 0.651683 | 5.3:+46323579 | MS.gene99941:CDS |
| TTTCTCAAAATGAACAAACA+CGG | 0.666895 | 5.3:+46323324 | None:intergenic |
| AACCGATGAAACCCACTACG+AGG | 0.717095 | 5.3:+46323365 | MS.gene99941:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 46323333 | 46331175 | 46323333 | ID=MS.gene99941 |
| chr5.3 | mRNA | 46323333 | 46331175 | 46323333 | ID=MS.gene99941.t1;Parent=MS.gene99941 |
| chr5.3 | exon | 46323333 | 46323434 | 46323333 | ID=MS.gene99941.t1.exon1;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46323333 | 46323434 | 46323333 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46323526 | 46323662 | 46323526 | ID=MS.gene99941.t1.exon2;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46323526 | 46323662 | 46323526 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46324902 | 46324968 | 46324902 | ID=MS.gene99941.t1.exon3;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46324902 | 46324968 | 46324902 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46325410 | 46325462 | 46325410 | ID=MS.gene99941.t1.exon4;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46325410 | 46325462 | 46325410 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46329072 | 46329126 | 46329072 | ID=MS.gene99941.t1.exon5;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46329072 | 46329126 | 46329072 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46329216 | 46329290 | 46329216 | ID=MS.gene99941.t1.exon6;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46329216 | 46329290 | 46329216 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46329378 | 46329495 | 46329378 | ID=MS.gene99941.t1.exon7;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46329378 | 46329495 | 46329378 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46330931 | 46330977 | 46330931 | ID=MS.gene99941.t1.exon8;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46330931 | 46330977 | 46330931 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| chr5.3 | exon | 46331083 | 46331175 | 46331083 | ID=MS.gene99941.t1.exon9;Parent=MS.gene99941.t1 |
| chr5.3 | CDS | 46331083 | 46331175 | 46331083 | ID=cds.MS.gene99941.t1;Parent=MS.gene99941.t1 |
| Gene Sequence |
| Protein sequence |
