AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048423.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048423.01.T01 MTR_5g061520 97.137 489 14 0 1 489 1 489 0.0 984
MsG0080048423.01.T01 MTR_1g086650 51.943 489 224 8 5 488 54 536 6.12e-172 495
MsG0080048423.01.T01 MTR_5g091660 49.684 475 220 8 15 480 64 528 5.75e-159 462
MsG0080048423.01.T01 MTR_3g448430 46.934 473 233 10 15 479 47 509 3.35e-147 431
MsG0080048423.01.T01 MTR_8g030620 42.798 486 256 8 8 479 18 495 1.94e-121 365
MsG0080048423.01.T01 MTR_4g415290 42.300 487 266 8 10 487 15 495 5.70e-121 363
MsG0080048423.01.T01 MTR_1g040500 41.010 495 272 11 8 487 10 499 1.63e-120 365
MsG0080048423.01.T01 MTR_5g080360 40.125 481 263 9 17 482 3 473 2.11e-116 352
MsG0080048423.01.T01 MTR_3g048330 58.929 280 108 3 210 485 1 277 5.11e-116 343
MsG0080048423.01.T01 MTR_7g067380 39.468 489 290 4 2 486 10 496 6.64e-116 350
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048423.01.T01 AT4G01950 51.653 484 221 10 11 484 40 520 5.46e-170 489
MsG0080048423.01.T01 AT4G01950 51.653 484 221 10 11 484 68 548 1.75e-169 489
MsG0080048423.01.T01 AT1G02390 50.204 490 221 8 8 480 43 526 1.28e-165 479
MsG0080048423.01.T01 AT1G06520 47.205 483 232 10 17 480 105 583 1.08e-149 440
MsG0080048423.01.T01 AT2G38110 42.227 476 260 9 14 479 22 492 3.96e-119 359
MsG0080048423.01.T01 AT3G11430 39.959 483 267 9 14 482 9 482 4.86e-119 358
MsG0080048423.01.T01 AT5G06090 40.329 486 271 12 9 482 2 480 3.16e-118 357
MsG0080048423.01.T01 AT1G01610 42.380 479 263 8 17 485 23 498 1.69e-116 352
MsG0080048423.01.T01 AT4G00400 41.949 472 261 7 17 479 23 490 5.77e-111 338
MsG0080048423.01.T01 AT3G11325 32.104 461 213 9 26 482 6 370 4.97e-72 234
MsG0080048423.01.T01 AT3G11325 32.104 461 213 9 26 482 9 373 5.29e-72 234

Find 88 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCTTGAAAGACTATTT+AGG 0.195935 contig312end:-13991 MsG0080048423.01.T01:CDS
TGTAATGAGGGAAAAGTTTC+TGG 0.203098 contig312end:+13815 None:intergenic
GTATAAGAAAAGACACTTTA+AGG 0.207106 contig312end:-14134 MsG0080048423.01.T01:CDS
GAGAATAGTATTGGTATTAT+TGG 0.218296 contig312end:-13859 MsG0080048423.01.T01:CDS
TCAACTAGGTTTGAAGATTA+TGG 0.218722 contig312end:-14175 MsG0080048423.01.T01:CDS
GAAAGACAAATATATGATTT+TGG 0.225097 contig312end:-12292 MsG0080048423.01.T01:CDS
ATTAAAACTAAGATAGAAAT+AGG 0.231887 contig312end:+12169 None:intergenic
AAATGAAATTGGAAAGAGTT+TGG 0.246873 contig312end:-12340 MsG0080048423.01.T01:CDS
AAACCTTCAACCATTATTCT+TGG 0.248292 contig312end:+14009 None:intergenic
AATTGTAGTTATACTTGTTT+TGG 0.249787 contig312end:+14432 None:intergenic
TTAACAAAGTAAACAGGATT+TGG 0.272628 contig312end:+12441 None:intergenic
GTCCCTGAAAATGCTAATAT+TGG 0.280666 contig312end:+12897 None:intergenic
ATTGGAGCTGATGCATTAAA+AGG 0.310915 contig312end:+12915 None:intergenic
TCTGGTCAATATACTCAATA+TGG 0.319173 contig312end:+13833 None:intergenic
TTTGTCTTTCCTAGTCAAAT+TGG 0.322666 contig312end:+12305 None:intergenic
AATGAAATTGGAAAGAGTTT+GGG 0.331774 contig312end:-12339 MsG0080048423.01.T01:CDS
TTTGTTTCTTTCATACCCTT+TGG 0.336221 contig312end:-14217 MsG0080048423.01.T01:CDS
ACATCACAAACATGTCAAAA+AGG 0.340009 contig312end:-12399 MsG0080048423.01.T01:CDS
TCTCTACAAGTTGTTCCTTC+TGG 0.351163 contig312end:+12609 None:intergenic
GTTCTGATGAGAGGAGAAAT+TGG 0.353548 contig312end:-13094 MsG0080048423.01.T01:CDS
GTGGATCAAGCAAAGTTCTA+TGG 0.367532 contig312end:+12799 None:intergenic
GTTTCTTGAAAGACTATTTA+GGG 0.373176 contig312end:-13990 MsG0080048423.01.T01:CDS
TACCCTTTGGTATGGTTAGT+TGG 0.374543 contig312end:-14204 MsG0080048423.01.T01:CDS
TCCTGTTGCTGTTGATGTTA+AGG 0.382374 contig312end:-12532 MsG0080048423.01.T01:CDS
TGACACACAAATTAACTTGT+AGG 0.389095 contig312end:+12209 None:intergenic
GCTTGTTGCTTTAACATCAT+AGG 0.399072 contig312end:+13885 None:intergenic
ATTGGAAAGAGTTTGGGATT+TGG 0.401189 contig312end:-12333 MsG0080048423.01.T01:CDS
TATAAGAAAAGACACTTTAA+GGG 0.412950 contig312end:-14133 MsG0080048423.01.T01:CDS
ACAAAATTAGCAACTTCAAC+AGG 0.421254 contig312end:+12366 None:intergenic
TTCTTTCATACCCTTTGGTA+TGG 0.423298 contig312end:-14212 MsG0080048423.01.T01:CDS
TAGTAAGTTACCAAGAATAA+TGG 0.425255 contig312end:-14019 MsG0080048423.01.T01:CDS
ACCTTAACATCAACAGCAAC+AGG 0.427108 contig312end:+12531 None:intergenic
GGACTAAACCTTAGCAAGTA+AGG 0.432275 contig312end:+12585 None:intergenic
CATCTTGCTGCAGGTAAGGT+TGG 0.435384 contig312end:+14389 None:intergenic
ATGCTAGTTTCCTTTGAAGC+TGG 0.441903 contig312end:-14261 MsG0080048423.01.T01:CDS
CTACTTAGAAGATGTTGGTT+GGG 0.444813 contig312end:-14085 MsG0080048423.01.T01:CDS
AGCTAGTGGATATAAATGTT+TGG 0.447892 contig312end:-12487 MsG0080048423.01.T01:CDS
TTACCAAGAATAATGGTTGA+AGG 0.459458 contig312end:-14012 MsG0080048423.01.T01:CDS
TCATGGAAAATCAATGGCTT+AGG 0.460797 contig312end:+13047 None:intergenic
AAGGAAACTAGCATGAAGTA+AGG 0.462736 contig312end:+14270 None:intergenic
TTAGAAGATGTTGGTTGGGA+AGG 0.468636 contig312end:-14081 MsG0080048423.01.T01:CDS
CGCGCGACCGAGCAAGAGAC+AGG 0.475152 contig312end:-12677 MsG0080048423.01.T01:CDS
ACATCTTCTAAGTAGAACTT+TGG 0.478280 contig312end:+14093 None:intergenic
AGCAAGAGACAGGGATTCAA+TGG 0.480606 contig312end:-12667 MsG0080048423.01.T01:CDS
CTAGTTTCCTTTGAAGCTGG+TGG 0.484232 contig312end:-14258 MsG0080048423.01.T01:CDS
CTTCCATCATGGAAAATCAA+TGG 0.487517 contig312end:+13041 None:intergenic
ATAGTGTGTGATATATAAAG+TGG 0.489764 contig312end:+12780 None:intergenic
TTGAATCCCTGTCTCTTGCT+CGG 0.491801 contig312end:+12670 None:intergenic
TGCTTTAACATCATAGGAAG+TGG 0.493418 contig312end:+13891 None:intergenic
TAGTATTCCACCAGCTTCAA+AGG 0.493486 contig312end:+14251 None:intergenic
TTCCTTCTGGACACACTACA+AGG 0.493940 contig312end:+12622 None:intergenic
ACAAGCAAAGAGAATAGTAT+TGG 0.508669 contig312end:-13868 MsG0080048423.01.T01:CDS
TCTACTTAGAAGATGTTGGT+TGG 0.515294 contig312end:-14086 MsG0080048423.01.T01:CDS
GTGGGTCAGTAGTGTGGTGA+GGG 0.516042 contig312end:-12125 MsG0080048423.01.T01:three_prime_UTR
CACCAACTAACCATACCAAA+GGG 0.528093 contig312end:+14202 None:intergenic
AGACACTTTAAGGGTAGGAA+TGG 0.534443 contig312end:-14124 MsG0080048423.01.T01:CDS
AGAAACCAAACCTTGTAATG+AGG 0.540345 contig312end:+13802 None:intergenic
GTAGAGAGCCTTACTTGCTA+AGG 0.541715 contig312end:-12593 MsG0080048423.01.T01:CDS
TGAAAGCCAATCTTCCATCA+TGG 0.549956 contig312end:+13030 None:intergenic
GAAACCAAACCTTGTAATGA+GGG 0.554140 contig312end:+13803 None:intergenic
AAGTTCTACTTAGAAGATGT+TGG 0.556652 contig312end:-14090 MsG0080048423.01.T01:CDS
ATGGAGAAAATGCTTTGTGA+AGG 0.565042 contig312end:-12648 MsG0080048423.01.T01:CDS
TTGGTTGCACCAATTTGACT+AGG 0.569693 contig312end:-12314 MsG0080048423.01.T01:CDS
TTATGTTAAGTTCTGATGAG+AGG 0.571837 contig312end:-13103 MsG0080048423.01.T01:CDS
GGTGGGTCAGTAGTGTGGTG+AGG 0.575100 contig312end:-12126 MsG0080048423.01.T01:three_prime_UTR
AGAAAAGACACTTTAAGGGT+AGG 0.579822 contig312end:-14129 MsG0080048423.01.T01:CDS
AGGTTGGTTCTTTAAAACAA+AGG 0.587296 contig312end:+14405 None:intergenic
ATGTTCATGTGGTTACCACT+TGG 0.589495 contig312end:-12981 MsG0080048423.01.T01:CDS
AACCTTGTAGTGTGTCCAGA+AGG 0.589664 contig312end:-12624 MsG0080048423.01.T01:CDS
AGGAAACTAGCATGAAGTAA+GGG 0.591307 contig312end:+14271 None:intergenic
AGTAATGAATATTACCTGGT+GGG 0.595121 contig312end:-12143 MsG0080048423.01.T01:three_prime_UTR
TCACCAACTAACCATACCAA+AGG 0.596402 contig312end:+14201 None:intergenic
ATTGAAAGAAATAGTCCAAG+TGG 0.598959 contig312end:+12966 None:intergenic
AACTTGCAGTTATTATGACA+AGG 0.600625 contig312end:+12233 None:intergenic
TTAAAGTAATGAATATTACC+TGG 0.601181 contig312end:-12147 MsG0080048423.01.T01:three_prime_UTR
TTAGTTGGTGAGTATCAACT+AGG 0.603020 contig312end:-14189 MsG0080048423.01.T01:CDS
ACTAGTGAAGATGTTACAGT+TGG 0.610755 contig312end:+13005 None:intergenic
CTTCACTAGTCATGTTCATG+TGG 0.612621 contig312end:-12992 MsG0080048423.01.T01:CDS
ACTTTGTTAAAATACTTGAG+AGG 0.622036 contig312end:-12428 MsG0080048423.01.T01:CDS
AAGTAATGAATATTACCTGG+TGG 0.625285 contig312end:-12144 MsG0080048423.01.T01:three_prime_UTR
CACCACACTACTGACCCACC+AGG 0.632024 contig312end:+12129 None:intergenic
GAAGTAAGGGAAGAGTGAAG+TGG 0.637079 contig312end:+14284 None:intergenic
CTATATGTGACTGCTGAGAG+TGG 0.637208 contig312end:+12750 None:intergenic
TTTCTTGAAAGACTATTTAG+GGG 0.640361 contig312end:-13989 MsG0080048423.01.T01:CDS
GCGCGACCGAGCAAGAGACA+GGG 0.649802 contig312end:-12676 MsG0080048423.01.T01:CDS
AGAGACATAAAGTCATACAA+TGG 0.657033 contig312end:-13949 MsG0080048423.01.T01:CDS
TCAGTTAGTTCAGCAAACAA+AGG 0.659246 contig312end:+12564 None:intergenic
TACCTGGTGGGTCAGTAGTG+TGG 0.678698 contig312end:-12131 MsG0080048423.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTAGATGTTTATAT+AGG - contig312end:14432-14451 MsG0080048423.01.T01:five_prime_UTR 10.0%
!! ATTAAAACTAAGATAGAAAT+AGG + contig312end:14315-14334 None:intergenic 15.0%
!! TATAAGAAAAGACACTTTAA+GGG - contig312end:12348-12367 MsG0080048423.01.T01:CDS 20.0%
!! TGTTTATACTTTCAAAATCA+TGG - contig312end:12720-12739 MsG0080048423.01.T01:CDS 20.0%
!! TTAAAGTAATGAATATTACC+TGG - contig312end:14334-14353 MsG0080048423.01.T01:CDS 20.0%
!!! AATTGTAGTTATACTTGTTT+TGG + contig312end:12052-12071 None:intergenic 20.0%
!!! AATTTTGTGCAAAATGAAAT+TGG - contig312end:14130-14149 MsG0080048423.01.T01:CDS 20.0%
!!! AGTATTTTAACAAAGTAAAC+AGG + contig312end:14049-14068 None:intergenic 20.0%
!!! CTTTTGATTTTTTTCGTTTT+CGG + contig312end:13642-13661 None:intergenic 20.0%
!!! GAAAGACAAATATATGATTT+TGG - contig312end:14189-14208 MsG0080048423.01.T01:CDS 20.0%
!!! TCAATTTTCAGAATAACATT+TGG - contig312end:13533-13552 MsG0080048423.01.T01:intron 20.0%
!!! TTTTAGACAACAAAAAAAGT+TGG + contig312end:13309-13328 None:intergenic 20.0%
! AAATGAAATTGGAAAGAGTT+TGG - contig312end:14141-14160 MsG0080048423.01.T01:CDS 25.0%
! AATGAAATTGGAAAGAGTTT+GGG - contig312end:14142-14161 MsG0080048423.01.T01:CDS 25.0%
! ACTTTGTTAAAATACTTGAG+AGG - contig312end:14053-14072 MsG0080048423.01.T01:CDS 25.0%
! ATAGTGTGTGATATATAAAG+TGG + contig312end:13704-13723 None:intergenic 25.0%
! GTATAAGAAAAGACACTTTA+AGG - contig312end:12347-12366 MsG0080048423.01.T01:CDS 25.0%
! GTTTCTTGAAAGACTATTTA+GGG - contig312end:12491-12510 MsG0080048423.01.T01:CDS 25.0%
! TAGTAAGTTACCAAGAATAA+TGG - contig312end:12462-12481 MsG0080048423.01.T01:CDS 25.0%
! TTAACAAAGTAAACAGGATT+TGG + contig312end:14043-14062 None:intergenic 25.0%
! TTTCTTGAAAGACTATTTAG+GGG - contig312end:12492-12511 MsG0080048423.01.T01:CDS 25.0%
!! AATGTTTTACAAAGAAAACG+AGG + contig312end:13100-13119 None:intergenic 25.0%
!! ATGGTTTTCTTAACCTTTTT+TGG - contig312end:12325-12344 MsG0080048423.01.T01:CDS 25.0%
!! GTCTTTTCTTATACCAAAAA+AGG + contig312end:12341-12360 None:intergenic 25.0%
!! GTTAAGGTTAGTATGTTTTA+TGG - contig312end:13965-13984 MsG0080048423.01.T01:CDS 25.0%
!!! ACACTAGTTTTTGATTTTGA+AGG - contig312end:12160-12179 MsG0080048423.01.T01:three_prime_UTR 25.0%
!!! GAGAATAGTATTGGTATTAT+TGG - contig312end:12622-12641 MsG0080048423.01.T01:CDS 25.0%
!!! TCATACAATGGTTACTTTTT+GGG - contig312end:12544-12563 MsG0080048423.01.T01:CDS 25.0%
!!! TGAAAGTTTAGAAGTTTTGT+TGG + contig312end:12134-12153 None:intergenic 25.0%
!!! TTAATCGCGTTGTTTTAATA+GGG + contig312end:13784-13803 None:intergenic 25.0%
AAACCTTCAACCATTATTCT+TGG + contig312end:12475-12494 None:intergenic 30.0%
AACTTGCAGTTATTATGACA+AGG + contig312end:14251-14270 None:intergenic 30.0%
AAGTAATGAATATTACCTGG+TGG - contig312end:14337-14356 MsG0080048423.01.T01:CDS 30.0%
AAGTTCTACTTAGAAGATGT+TGG - contig312end:12391-12410 MsG0080048423.01.T01:CDS 30.0%
ACAAAATTAGCAACTTCAAC+AGG + contig312end:14118-14137 None:intergenic 30.0%
ACAAGCAAAGAGAATAGTAT+TGG - contig312end:12613-12632 MsG0080048423.01.T01:CDS 30.0%
ACATCACAAACATGTCAAAA+AGG - contig312end:14082-14101 MsG0080048423.01.T01:CDS 30.0%
ACATCTTCTAAGTAGAACTT+TGG + contig312end:12391-12410 None:intergenic 30.0%
AGAGACATAAAGTCATACAA+TGG - contig312end:12532-12551 MsG0080048423.01.T01:CDS 30.0%
AGTAATGAATATTACCTGGT+GGG - contig312end:14338-14357 MsG0080048423.01.T01:CDS 30.0%
ATTGAAAGAAATAGTCCAAG+TGG + contig312end:13518-13537 None:intergenic 30.0%
GGATTCAAGTAATGAAAAAC+AGG + contig312end:14022-14041 None:intergenic 30.0%
GGTTTCTTGAAAGACTATTT+AGG - contig312end:12490-12509 MsG0080048423.01.T01:CDS 30.0%
TCAACTAGGTTTGAAGATTA+TGG - contig312end:12306-12325 MsG0080048423.01.T01:CDS 30.0%
TCTGGTCAATATACTCAATA+TGG + contig312end:12651-12670 None:intergenic 30.0%
TGACACAAAAACCAAAACAA+CGG + contig312end:13074-13093 None:intergenic 30.0%
TGTTTATAGTCGCTAATCAA+CGG + contig312end:12848-12867 None:intergenic 30.0%
TTACCAAGAATAATGGTTGA+AGG - contig312end:12469-12488 MsG0080048423.01.T01:CDS 30.0%
TTTGTCTTTCCTAGTCAAAT+TGG + contig312end:14179-14198 None:intergenic 30.0%
! AGCTAGTGGATATAAATGTT+TGG - contig312end:13994-14013 MsG0080048423.01.T01:CDS 30.0%
! CTCCAATATTAGCATTTTCA+GGG - contig312end:13582-13601 MsG0080048423.01.T01:intron 30.0%
! TTATGTTAAGTTCTGATGAG+AGG - contig312end:13378-13397 MsG0080048423.01.T01:intron 30.0%
! TTTGTTTCTTTCATACCCTT+TGG - contig312end:12264-12283 MsG0080048423.01.T01:CDS 30.0%
! TTTTGACATGTTTGTGATGT+TGG + contig312end:14082-14101 None:intergenic 30.0%
!! AGGTTGGTTCTTTAAAACAA+AGG + contig312end:12079-12098 None:intergenic 30.0%
!! GACAAATATATGATTTTGGC+AGG - contig312end:14193-14212 MsG0080048423.01.T01:CDS 30.0%
!! TGACACACAAATTAACTTGT+AGG + contig312end:14275-14294 None:intergenic 30.0%
!!! ACAAGGTTTGGTTTCTTTTT+TGG - contig312end:12686-12705 MsG0080048423.01.T01:CDS 30.0%
!!! GTCATACAATGGTTACTTTT+TGG - contig312end:12543-12562 MsG0080048423.01.T01:CDS 30.0%
!!! GTTAATCGCGTTGTTTTAAT+AGG + contig312end:13785-13804 None:intergenic 30.0%
!!! TAATCGCGTTGTTTTAATAG+GGG + contig312end:13783-13802 None:intergenic 30.0%
AAGGAAACTAGCATGAAGTA+AGG + contig312end:12214-12233 None:intergenic 35.0%
ACTAGTGAAGATGTTACAGT+TGG + contig312end:13479-13498 None:intergenic 35.0%
AGAAACCAAACCTTGTAATG+AGG + contig312end:12682-12701 None:intergenic 35.0%
AGGAAACTAGCATGAAGTAA+GGG + contig312end:12213-12232 None:intergenic 35.0%
ATGGAGAAAATGCTTTGTGA+AGG - contig312end:13833-13852 MsG0080048423.01.T01:CDS 35.0%
ATTGGAGCTGATGCATTAAA+AGG + contig312end:13569-13588 None:intergenic 35.0%
CTTCCATCATGGAAAATCAA+TGG + contig312end:13443-13462 None:intergenic 35.0%
GAAACCAAACCTTGTAATGA+GGG + contig312end:12681-12700 None:intergenic 35.0%
GATTTCGCGGTTTAATACTA+CGG - contig312end:12938-12957 MsG0080048423.01.T01:CDS 35.0%
GTCCCTGAAAATGCTAATAT+TGG + contig312end:13587-13606 None:intergenic 35.0%
TCACAAACATGTCAAAAAGG+TGG - contig312end:14085-14104 MsG0080048423.01.T01:CDS 35.0%
TCAGTTAGTTCAGCAAACAA+AGG + contig312end:13920-13939 None:intergenic 35.0%
TCATGGAAAATCAATGGCTT+AGG + contig312end:13437-13456 None:intergenic 35.0%
TCTACTTAGAAGATGTTGGT+TGG - contig312end:12395-12414 MsG0080048423.01.T01:CDS 35.0%
TGCATTATTGTCTGATTTCG+CGG - contig312end:12925-12944 MsG0080048423.01.T01:CDS 35.0%
TGCTTTAACATCATAGGAAG+TGG + contig312end:12593-12612 None:intergenic 35.0%
TGTAATGAGGGAAAAGTTTC+TGG + contig312end:12669-12688 None:intergenic 35.0%
TTCTTTCATACCCTTTGGTA+TGG - contig312end:12269-12288 MsG0080048423.01.T01:CDS 35.0%
! AGAAAAGACACTTTAAGGGT+AGG - contig312end:12352-12371 MsG0080048423.01.T01:CDS 35.0%
! ATTGGAAAGAGTTTGGGATT+TGG - contig312end:14148-14167 MsG0080048423.01.T01:CDS 35.0%
! CTACTTAGAAGATGTTGGTT+GGG - contig312end:12396-12415 MsG0080048423.01.T01:CDS 35.0%
! GAAACTTTTCCCTCATTACA+AGG - contig312end:12669-12688 MsG0080048423.01.T01:CDS 35.0%
! GCTCCAATATTAGCATTTTC+AGG - contig312end:13581-13600 MsG0080048423.01.T01:intron 35.0%
! TTTTATGGAACAACAGCTAG+TGG - contig312end:13980-13999 MsG0080048423.01.T01:CDS 35.0%
! TTTTCCCTCATTACAAGGTT+TGG - contig312end:12674-12693 MsG0080048423.01.T01:CDS 35.0%
! TTTTTCTCTCTCCGTTGTTT+TGG - contig312end:13060-13079 MsG0080048423.01.T01:CDS 35.0%
!! ATGATTTTGGCAGGTAATGA+AGG - contig312end:14202-14221 MsG0080048423.01.T01:CDS 35.0%
!! GATTTTCCATGATGGAAGAT+TGG - contig312end:13445-13464 MsG0080048423.01.T01:intron 35.0%
!! GCTTGTTGCTTTAACATCAT+AGG + contig312end:12599-12618 None:intergenic 35.0%
!! TTAGTTGGTGAGTATCAACT+AGG - contig312end:12292-12311 MsG0080048423.01.T01:CDS 35.0%
!!! AAGCCATTGATTTTCCATGA+TGG - contig312end:13437-13456 MsG0080048423.01.T01:intron 35.0%
!!! AAGTTTTGTTGGCAAGTGAA+AGG + contig312end:12123-12142 None:intergenic 35.0%
!!! ATTTTTTTCGTTTTCGGTCG+AGG + contig312end:13636-13655 None:intergenic 35.0%
!!! GGCTTAGGAAACAACTTTTT+TGG + contig312end:13422-13441 None:intergenic 35.0%
ACCTTAACATCAACAGCAAC+AGG + contig312end:13953-13972 None:intergenic 40.0%
ATACTGCGATTCCTGATTGT+AGG - contig312end:13216-13235 MsG0080048423.01.T01:intron 40.0%
ATGTTCATGTGGTTACCACT+TGG - contig312end:13500-13519 MsG0080048423.01.T01:intron 40.0%
CACCAACTAACCATACCAAA+GGG + contig312end:12282-12301 None:intergenic 40.0%
CTTCACTAGTCATGTTCATG+TGG - contig312end:13489-13508 MsG0080048423.01.T01:intron 40.0%
GGACTAAACCTTAGCAAGTA+AGG + contig312end:13899-13918 None:intergenic 40.0%
GTGGATCAAGCAAAGTTCTA+TGG + contig312end:13685-13704 None:intergenic 40.0%
GTTCTGATGAGAGGAGAAAT+TGG - contig312end:13387-13406 MsG0080048423.01.T01:intron 40.0%
TACCCTTTGGTATGGTTAGT+TGG - contig312end:12277-12296 MsG0080048423.01.T01:CDS 40.0%
TACTGCGATTCCTGATTGTA+GGG - contig312end:13217-13236 MsG0080048423.01.T01:intron 40.0%
TAGTATTCCACCAGCTTCAA+AGG + contig312end:12233-12252 None:intergenic 40.0%
TATAGTCGCTAATCAACGGA+AGG + contig312end:12844-12863 None:intergenic 40.0%
TCACCAACTAACCATACCAA+AGG + contig312end:12283-12302 None:intergenic 40.0%
TCAGAGCTTCAGAAAAACGA+GGG + contig312end:12789-12808 None:intergenic 40.0%
TCTCTACAAGTTGTTCCTTC+TGG + contig312end:13875-13894 None:intergenic 40.0%
TGAAAGCCAATCTTCCATCA+TGG + contig312end:13454-13473 None:intergenic 40.0%
TTCAGAGCTTCAGAAAAACG+AGG + contig312end:12790-12809 None:intergenic 40.0%
TTGGTTGCACCAATTTGACT+AGG - contig312end:14167-14186 MsG0080048423.01.T01:CDS 40.0%
! AAGGTTTTTCATCTTGCTGC+AGG + contig312end:12104-12123 None:intergenic 40.0%
! AGACACTTTAAGGGTAGGAA+TGG - contig312end:12357-12376 MsG0080048423.01.T01:CDS 40.0%
! TCCTGTTGCTGTTGATGTTA+AGG - contig312end:13949-13968 MsG0080048423.01.T01:CDS 40.0%
! TTAGAAGATGTTGGTTGGGA+AGG - contig312end:12400-12419 MsG0080048423.01.T01:CDS 40.0%
! TTTTCATCTTGCTGCAGGTA+AGG + contig312end:12099-12118 None:intergenic 40.0%
!! ATGCTAGTTTCCTTTGAAGC+TGG - contig312end:12220-12239 MsG0080048423.01.T01:three_prime_UTR 40.0%
AACCTTGTAGTGTGTCCAGA+AGG - contig312end:13857-13876 MsG0080048423.01.T01:CDS 45.0%
AGCAAGAGACAGGGATTCAA+TGG - contig312end:13814-13833 MsG0080048423.01.T01:CDS 45.0%
CTATATGTGACTGCTGAGAG+TGG + contig312end:13734-13753 None:intergenic 45.0%
GAAGTAAGGGAAGAGTGAAG+TGG + contig312end:12200-12219 None:intergenic 45.0%
GACATGCATTCCCTACAATC+AGG + contig312end:13230-13249 None:intergenic 45.0%
TGCATGTCCCAAAATCACGA+TGG - contig312end:13242-13261 MsG0080048423.01.T01:intron 45.0%
TTCCTTCTGGACACACTACA+AGG + contig312end:13862-13881 None:intergenic 45.0%
TTGAATCCCTGTCTCTTGCT+CGG + contig312end:13814-13833 None:intergenic 45.0%
! ATAGCGTGCCATCGTGATTT+TGG + contig312end:13253-13272 None:intergenic 45.0%
! CGTTTTCGGTCGAGGTTTTA+GGG + contig312end:13628-13647 None:intergenic 45.0%
! GTAGAGAGCCTTACTTGCTA+AGG - contig312end:13888-13907 MsG0080048423.01.T01:CDS 45.0%
! TAGCGTGCCATCGTGATTTT+GGG + contig312end:13252-13271 None:intergenic 45.0%
! TCGTTTTCGGTCGAGGTTTT+AGG + contig312end:13629-13648 None:intergenic 45.0%
!! CTAGTTTCCTTTGAAGCTGG+TGG - contig312end:12223-12242 MsG0080048423.01.T01:three_prime_UTR 45.0%
CATCTTGCTGCAGGTAAGGT+TGG + contig312end:12095-12114 None:intergenic 50.0%
GTGGGTCAGTAGTGTGGTGA+GGG - contig312end:14356-14375 MsG0080048423.01.T01:CDS 55.0%
! TACCTGGTGGGTCAGTAGTG+TGG - contig312end:14350-14369 MsG0080048423.01.T01:CDS 55.0%
CACCACACTACTGACCCACC+AGG + contig312end:14355-14374 None:intergenic 60.0%
GGTGGGTCAGTAGTGTGGTG+AGG - contig312end:14355-14374 MsG0080048423.01.T01:CDS 60.0%
GCGCGACCGAGCAAGAGACA+GGG - contig312end:13805-13824 MsG0080048423.01.T01:intron 65.0%
CGCGCGACCGAGCAAGAGAC+AGG - contig312end:13804-13823 MsG0080048423.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
contig312end gene 12030 14473 12030 ID=MsG0080048423.01;Name=MsG0080048423.01
contig312end mRNA 12030 14473 12030 ID=MsG0080048423.01.T01;Parent=MsG0080048423.01;Name=MsG0080048423.01.T01;_AED=0.17;_eAED=0.17;_QI=82|1|1|1|1|1|2|215|489
contig312end exon 12030 13135 12030 ID=MsG0080048423.01.T01:exon:4503;Parent=MsG0080048423.01.T01
contig312end exon 13813 14473 13813 ID=MsG0080048423.01.T01:exon:4502;Parent=MsG0080048423.01.T01
contig312end five_prime_UTR 14392 14473 14392 ID=MsG0080048423.01.T01:five_prime_utr;Parent=MsG0080048423.01.T01
contig312end CDS 13813 14391 13813 ID=MsG0080048423.01.T01:cds;Parent=MsG0080048423.01.T01
contig312end CDS 12245 13135 12245 ID=MsG0080048423.01.T01:cds;Parent=MsG0080048423.01.T01
contig312end three_prime_UTR 12030 12244 12030 ID=MsG0080048423.01.T01:three_prime_utr;Parent=MsG0080048423.01.T01
Gene Sequence

>MsG0080048423.01.T01

ATGAAAAACCTTTCACTTGCCAACAAAACTTCTAAACTTTCAAATAAAACACTAGTTTTTGATTTTGAAGGCACTCTTCTAAGATCCACTTCACTCTTCCCTTACTTCATGCTAGTTTCCTTTGAAGCTGGTGGAATACTAAGATCTATCATTTTGTTTCTTTCATACCCTTTGGTATGGTTAGTTGGTGAGTATCAACTAGGTTTGAAGATTATGGTTTTCTTAACCTTTTTTGGTATAAGAAAAGACACTTTAAGGGTAGGAATGGCTGTTCTTCCAAAGTTCTACTTAGAAGATGTTGGTTGGGAAGGATTTGAAGCTGTGATGTGTTGTGAGAGAAAAGTTGCATCTAGTAAGTTACCAAGAATAATGGTTGAAGGTTTCTTGAAAGACTATTTAGGGGTTGAAGCTATTGTAGCAAGAGACATAAAGTCATACAATGGTTACTTTTTGGGTTTGTTTGAGAAACATAAAACAACCACTTCCTATGATGTTAAAGCAACAAGCAAAGAGAATAGTATTGGTATTATTGGTAGCCATATTGAGTATATTGACCAGAAACTTTTCCCTCATTACAAGAAGATTTGCTTTATGTTAAGTTCTGATGAGAGGAGAAATTGGCGTGTATTACCAAAAAAGTTGTTTCCTAAGCCATTGATTTTCCATGATGGAAGATTGGCTTTCATGCCAACTGTAACATCTTCACTAGTCATGTTCATGTGGTTACCACTTGGACTATTTCTTTCAATTTTCAGAATAACATTTGGAGTTTCATTACCTTTTAATGCATCAGCTCCAATATTAGCATTTTCAGGGACAAGAAGCACAATTTCAAGCCCTAAAACCTCGACCGAAAACGAAAAAAATCAAAAGAATATGCTTTATGTGTGTAACCATAGAACTTTGCTTGATCCACTTTATATATCACACACTATAAACAAACCACTCTCAGCAGTCACATATAGCATGAGCAGAGTCAATGAGATTATTTCCCCTATTAAAACAACGCGATTAACGCGCGACCGAGCAAGAGACAGGGATTCAATGGAGAAAATGCTTTGTGAAGGAAACCTTGTAGTGTGTCCAGAAGGAACAACTTGTAGAGAGCCTTACTTGCTAAGGTTTAGTCCTTTGTTTGCTGAACTAACTGATGATATTGTTCCTGTTGCTGTTGATGTTAAGGTTAGTATGTTTTATGGAACAACAGCTAGTGGATATAAATGTTTGGATCCTGTTTTTCATTACTTGAATCCAAATCCTGTTTACTTTGTTAAAATACTTGAGAGGATACCAACATCACAAACATGTCAAAAAGGTGGAAAATCTCCTGTTGAAGTTGCTAATTTTGTGCAAAATGAAATTGGAAAGAGTTTGGGATTTGGTTGCACCAATTTGACTAGGAAAGACAAATATATGATTTTGGCAGGTAATGAAGGTGTGAGTAGCAATAACAAACAATCCTTGTCATAA
Protein sequence

>MsG0080048423.01.T01

MKNLSLANKTSKLSNKTLVFDFEGTLLRSTSLFPYFMLVSFEAGGILRSIILFLSYPLVWLVGEYQLGLKIMVFLTFFGIRKDTLRVGMAVLPKFYLEDVGWEGFEAVMCCERKVASSKLPRIMVEGFLKDYLGVEAIVARDIKSYNGYFLGLFEKHKTTTSYDVKATSKENSIGIIGSHIEYIDQKLFPHYKKICFMLSSDERRNWRVLPKKLFPKPLIFHDGRLAFMPTVTSSLVMFMWLPLGLFLSIFRITFGVSLPFNASAPILAFSGTRSTISSPKTSTENEKNQKNMLYVCNHRTLLDPLYISHTINKPLSAVTYSMSRVNEIISPIKTTRLTRDRARDRDSMEKMLCEGNLVVCPEGTTCREPYLLRFSPLFAELTDDIVPVAVDVKVSMFYGTTASGYKCLDPVFHYLNPNPVYFVKILERIPTSQTCQKGGKSPVEVANFVQNEIGKSLGFGCTNLTRKDKYMILAGNEGVSSNNKQSLS*