AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048707.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048707.01.T01 MTR_4g075480 91.613 310 7 3 1 291 1 310 0.0 567
MsG0080048707.01.T01 MTR_4g075480 96.364 275 3 2 24 291 8 282 0.0 533
MsG0080048707.01.T01 MTR_1g032600 52.536 276 106 7 24 281 79 347 2.32e-82 252
MsG0080048707.01.T01 MTR_4g081370 36.000 175 85 8 27 193 37 192 3.48e-18 80.9
MsG0080048707.01.T01 MTR_4g081370 42.188 128 50 5 27 146 81 192 2.31e-17 79.7
MsG0080048707.01.T01 MTR_2g082640 48.936 94 40 3 27 112 74 167 5.76e-16 75.9
MsG0080048707.01.T01 MTR_4g108360 43.434 99 49 2 24 115 52 150 1.49e-14 72.0
MsG0080048707.01.T01 MTR_1g043430 36.970 165 77 8 9 153 70 227 5.09e-13 67.8
MsG0080048707.01.T01 MTR_2g089000 43.158 95 46 3 27 113 110 204 1.12e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048707.01.T01 AT3G19860 60.408 245 76 7 24 255 7 243 1.42e-84 255
MsG0080048707.01.T01 AT3G19860 60.408 245 76 7 24 255 38 274 2.99e-84 256
MsG0080048707.01.T01 AT3G19860 60.000 245 77 7 24 255 60 296 6.39e-84 256
MsG0080048707.01.T01 AT4G36060 41.429 280 107 9 20 283 24 262 3.02e-46 157
MsG0080048707.01.T01 AT4G36060 41.429 280 107 9 20 283 42 280 1.61e-45 155
MsG0080048707.01.T01 AT4G36060 41.667 240 91 7 20 244 24 229 4.53e-44 151
MsG0080048707.01.T01 AT4G36060 41.667 240 91 7 20 244 42 247 2.80e-43 149
MsG0080048707.01.T01 AT5G54680 34.810 158 68 5 27 176 76 206 5.08e-15 73.2
MsG0080048707.01.T01 AT4G14410 35.669 157 71 8 2 141 112 255 5.84e-13 68.2
MsG0080048707.01.T01 AT4G14410 35.669 157 71 8 2 141 112 255 5.84e-13 68.2
MsG0080048707.01.T01 AT4G14410 35.669 157 71 8 2 141 118 261 9.69e-13 67.4
MsG0080048707.01.T01 AT3G23210 34.091 132 72 4 27 143 138 269 1.37e-12 67.0
MsG0080048707.01.T01 AT3G23210 34.091 132 72 4 27 143 167 298 3.34e-12 66.2
MsG0080048707.01.T01 AT1G51070 42.553 94 46 3 27 112 71 164 3.93e-12 65.1
MsG0080048707.01.T01 AT1G51070 42.553 94 46 3 27 112 137 230 6.34e-12 65.1

Find 115 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGAGAGTTTGTGATTA+AGG 0.196287 contig406end:-107495 None:intergenic
TTATCTAGAGTAGATGTATT+TGG 0.223138 contig406end:-107644 None:intergenic
AATGATAAAGCGACCATTCT+AGG 0.234923 contig406end:+106258 MsG0080048707.01.T01:CDS
AATTGTATTCGGAGCTAAAA+AGG 0.247255 contig406end:-106720 None:intergenic
TATTTCATCTAGTCTTTGTT+TGG 0.256862 contig406end:+107529 MsG0080048707.01.T01:three_prime_UTR
CGAACTACCTTCTGTGAAAC+TGG 0.280327 contig406end:-107069 None:intergenic
ATTTAGTGGGTTATAGTTCT+TGG 0.293825 contig406end:+108088 MsG0080048707.01.T01:three_prime_UTR
TAATCACAAACTCTCCTAAA+AGG 0.301913 contig406end:+107498 MsG0080048707.01.T01:three_prime_UTR
TATTGTTGTGTTCTCTTTGT+TGG 0.310430 contig406end:+108175 MsG0080048707.01.T01:three_prime_UTR
TCCTTAGTTGCAGCTGATAC+TGG 0.331417 contig406end:-106529 None:intergenic
ACTTTGAAGTGTGTTGAATA+AGG 0.335851 contig406end:+108521 MsG0080048707.01.T01:three_prime_UTR
TTCAATTTGTCACAAGGAAT+AGG 0.340147 contig406end:-107413 None:intergenic
TGTTGTTCAACAATTGTATT+CGG 0.342434 contig406end:-106731 None:intergenic
TTCTTCATCTCACAGTGTTC+AGG 0.343603 contig406end:+107103 MsG0080048707.01.T01:CDS
GGTAGCCGGTCCCACGTATC+AGG 0.344490 contig406end:+106788 MsG0080048707.01.T01:CDS
CACATACACTTCAATGTGTA+TGG 0.355745 contig406end:-107730 None:intergenic
ACCAGTATCAGCTGCAACTA+AGG 0.363603 contig406end:+106528 MsG0080048707.01.T01:CDS
CATTTGTATCTACAATTGAT+AGG 0.363857 contig406end:-108271 None:intergenic
TTAGGAGAGTTTGTGATTAA+GGG 0.370502 contig406end:-107494 None:intergenic
GATACGTGGGACCGGCTACC+TGG 0.378928 contig406end:-106785 None:intergenic
AATACATCTACTCTAGATAA+TGG 0.383584 contig406end:+107647 MsG0080048707.01.T01:three_prime_UTR
CTACTCTAGATAATGGATAA+TGG 0.388355 contig406end:+107654 MsG0080048707.01.T01:three_prime_UTR
ACATATGTTGAACAAGGGTT+AGG 0.393166 contig406end:-106695 None:intergenic
TCTGAAATGCAGACCCTGAT+AGG 0.394717 contig406end:+106229 MsG0080048707.01.T01:intron
AGGATGTTGATTAGCATAGT+AGG 0.395188 contig406end:-106663 None:intergenic
TCTTTGAGCCTTGCTCTCCC+TGG 0.396650 contig406end:-106837 None:intergenic
GCAGGTACCGGCATTGGAAC+TGG 0.401184 contig406end:-106614 None:intergenic
TCCTGGGTCTTCCGCAGATC+AGG 0.409087 contig406end:+106904 MsG0080048707.01.T01:CDS
GACCTTGAGTTGAAGACTCC+TGG 0.425984 contig406end:+106887 MsG0080048707.01.T01:CDS
GCAATTCTAAATCAATTTAG+TGG 0.428527 contig406end:+108074 MsG0080048707.01.T01:three_prime_UTR
GTTTACTAACTTGAGAAGTA+AGG 0.433635 contig406end:-106305 None:intergenic
TTCTTTGAGTTGTGAGGGAA+AGG 0.439952 contig406end:+107364 MsG0080048707.01.T01:three_prime_UTR
TCTGTTCTCTCTTCTTAACT+AGG 0.442611 contig406end:+107301 MsG0080048707.01.T01:three_prime_UTR
CGCCTCTTCATCCAGGTAGC+CGG 0.455426 contig406end:+106774 MsG0080048707.01.T01:CDS
ATAACGGAATGATCCATTGC+AGG 0.461908 contig406end:-106566 None:intergenic
AAATGATCTCAGAGAAGAAA+AGG 0.463510 contig406end:+106472 MsG0080048707.01.T01:CDS
GGCTACCTGGATGAAGAGGC+GGG 0.476225 contig406end:-106772 None:intergenic
TTCACAGAAGGTAGTTCGTT+AGG 0.482876 contig406end:+107074 MsG0080048707.01.T01:CDS
GAACCAGCAGGTACCGGCAT+TGG 0.486966 contig406end:-106620 None:intergenic
AGGCTGACCGAGAAAAGTTA+AGG 0.489877 contig406end:+105914 MsG0080048707.01.T01:CDS
GTTCCAATGCCGGTACCTGC+TGG 0.492349 contig406end:+106617 MsG0080048707.01.T01:CDS
GGATGTTGATTAGCATAGTA+GGG 0.493252 contig406end:-106662 None:intergenic
TGAGAGCAAAGAATTATCAT+TGG 0.498322 contig406end:-108544 None:intergenic
CATATGTTGAACAAGGGTTA+GGG 0.508234 contig406end:-106694 None:intergenic
ACCGGCATTGGAACTGGATA+CGG 0.508537 contig406end:-106608 None:intergenic
ACCTTGAGTTGAAGACTCCT+GGG 0.508601 contig406end:+106888 MsG0080048707.01.T01:CDS
AGCTCATCAAGTAGTGTAGT+GGG 0.509577 contig406end:+107128 MsG0080048707.01.T01:CDS
GAAAATGTTGAACGTACGTC+TGG 0.514766 contig406end:+107561 MsG0080048707.01.T01:three_prime_UTR
CAATTCTAAATCAATTTAGT+GGG 0.524459 contig406end:+108075 MsG0080048707.01.T01:three_prime_UTR
TCCGTATCCAGTTCCAATGC+CGG 0.524495 contig406end:+106607 MsG0080048707.01.T01:CDS
AGACCAGTGTGGCTAAATGT+AGG 0.525327 contig406end:+107462 MsG0080048707.01.T01:three_prime_UTR
GAATGATCCATTGCAGGCCA+TGG 0.527462 contig406end:-106560 None:intergenic
ACCCTCACAGTAGCTTCAAA+AGG 0.527695 contig406end:-107204 None:intergenic
TGACAAATTGAAATTGCAGA+TGG 0.531031 contig406end:+107424 MsG0080048707.01.T01:three_prime_UTR
CAAATCATCCAGGGAGAGCA+AGG 0.531527 contig406end:+106829 MsG0080048707.01.T01:CDS
GAAAGGCTAGTGAATGAGAA+TGG 0.532624 contig406end:+107156 MsG0080048707.01.T01:exon
AGCATGGTCTTGACTGATGA+GGG 0.533829 contig406end:+108478 MsG0080048707.01.T01:three_prime_UTR
TGCAATTTCAATTTGTCACA+AGG 0.534577 contig406end:-107419 None:intergenic
ATCTAGTAAGTCGTCGAGGA+AGG 0.538754 contig406end:+107043 MsG0080048707.01.T01:CDS
TTGAACTGTATCTCCTAGAA+TGG 0.541064 contig406end:-106271 None:intergenic
AATGGATCATTCCGTTATGA+TGG 0.543467 contig406end:+106571 MsG0080048707.01.T01:CDS
GGAATTGAACCAGCAGGTAC+CGG 0.545624 contig406end:-106626 None:intergenic
ACATACACTTCAATGTGTAT+GGG 0.546581 contig406end:-107729 None:intergenic
GTGAGGGATATGATGTACTA+TGG 0.547723 contig406end:+107750 MsG0080048707.01.T01:three_prime_UTR
TATTGCATTTGTGTTTACAA+TGG 0.547907 contig406end:+107904 MsG0080048707.01.T01:three_prime_UTR
TTGAATTTGAATGATTCGTA+TGG 0.551557 contig406end:+108445 MsG0080048707.01.T01:three_prime_UTR
ACCTGCTAGAAAGACACAAA+AGG 0.554651 contig406end:+105894 MsG0080048707.01.T01:intron
GTATCCCCGCCTCTTCATCC+AGG 0.556309 contig406end:+106767 MsG0080048707.01.T01:CDS
TATGTATCCATGGCCTGCAA+TGG 0.558315 contig406end:+106553 MsG0080048707.01.T01:CDS
TAGTAGGGGTACGGCTGCAT+GGG 0.560037 contig406end:-106647 None:intergenic
ATAGTAGGGGTACGGCTGCA+TGG 0.562866 contig406end:-106648 None:intergenic
AACTAAGGACTATGTATCCA+TGG 0.564461 contig406end:+106543 MsG0080048707.01.T01:CDS
CGGCTACCTGGATGAAGAGG+CGG 0.573692 contig406end:-106773 None:intergenic
ACCCAGGAGTCTTCAACTCA+AGG 0.576772 contig406end:-106889 None:intergenic
TAGTGTAGTGGGGAGCAGAA+AGG 0.578575 contig406end:+107139 MsG0080048707.01.T01:CDS
TAGCTCATCAAGTAGTGTAG+TGG 0.582065 contig406end:+107127 MsG0080048707.01.T01:CDS
TTATTGGCTTCATAATGTGT+AGG 0.582773 contig406end:+105660 None:intergenic
CATACACTTCAATGTGTATG+GGG 0.583024 contig406end:-107728 None:intergenic
TGATTAGCATAGTAGGGGTA+CGG 0.592321 contig406end:-106656 None:intergenic
ATAACTCAAATCTTGTGCGA+GGG 0.593290 contig406end:+108045 MsG0080048707.01.T01:three_prime_UTR
AAACCTACATTTAGCCACAC+TGG 0.600095 contig406end:-107465 None:intergenic
TATAACTCAAATCTTGTGCG+AGG 0.601125 contig406end:+108044 MsG0080048707.01.T01:three_prime_UTR
TTAAGAAGAGAGAACAGAAG+CGG 0.601746 contig406end:-107296 None:intergenic
AAGGGAACATATGTTGAACA+AGG 0.603401 contig406end:-106701 None:intergenic
GTTGAACGTACGTCTGGACT+CGG 0.603755 contig406end:+107567 MsG0080048707.01.T01:three_prime_UTR
GACCGGCTACCTGGATGAAG+AGG 0.604106 contig406end:-106776 None:intergenic
ATATTGCTGATGATAATGTA+CGG 0.604290 contig406end:-108396 None:intergenic
TTCTGCTGCAACTATTGTAG+AGG 0.604657 contig406end:+107238 MsG0080048707.01.T01:three_prime_UTR
GGAACTGGATACGGATATGT+TGG 0.606135 contig406end:-106599 None:intergenic
AAGGAAACCAGTTTCACAGA+AGG 0.607572 contig406end:+107062 MsG0080048707.01.T01:CDS
ACCTGATCTGCGGAAGACCC+AGG 0.614780 contig406end:-106905 None:intergenic
AGATACAGTTCAATTGCTGA+AGG 0.616836 contig406end:+106281 MsG0080048707.01.T01:CDS
AAGCATGGTCTTGACTGATG+AGG 0.617168 contig406end:+108477 MsG0080048707.01.T01:three_prime_UTR
TACACATTGAAGTGTATGTG+AGG 0.620194 contig406end:+107733 MsG0080048707.01.T01:three_prime_UTR
TGTTGGTGGAGCCATCATAA+CGG 0.621484 contig406end:-106582 None:intergenic
CAAGACAAGACTATAATCAC+AGG 0.622596 contig406end:-107792 None:intergenic
CAAGGGTTAGGGATGACTGC+AGG 0.622814 contig406end:-106683 None:intergenic
AGGCGGGGATACGTATTGAG+TGG 0.624785 contig406end:-106756 None:intergenic
AAGACTAGATGAAATAAGCA+TGG 0.625916 contig406end:-107522 None:intergenic
GAGCAAGGCTCAAAGAAATG+AGG 0.629493 contig406end:+106844 MsG0080048707.01.T01:CDS
CTGACCGAGAAAAGTTAAGG+AGG 0.634700 contig406end:+105917 MsG0080048707.01.T01:CDS
AGCAGCACTAACCTGATCTG+CGG 0.637188 contig406end:-106915 None:intergenic
GTATGGTTGCATCAAAAGCA+TGG 0.638328 contig406end:+108462 MsG0080048707.01.T01:three_prime_UTR
TGCATGGGAATTGAACCAGC+AGG 0.642923 contig406end:-106632 None:intergenic
ACACATTGAAGTGTATGTGA+GGG 0.644996 contig406end:+107734 MsG0080048707.01.T01:three_prime_UTR
TGACCGAGAAAAGTTAAGGA+GGG 0.651662 contig406end:+105918 MsG0080048707.01.T01:CDS
GCTAAATGAAGAATCTCGTG+AGG 0.654605 contig406end:+106347 MsG0080048707.01.T01:CDS
TGTGAGGGAAAGGCTGCGTG+AGG 0.655698 contig406end:+107374 MsG0080048707.01.T01:three_prime_UTR
ACTGGATACGGATATGTTGG+TGG 0.657323 contig406end:-106596 None:intergenic
AGTCTTCAACTCAAGGTCTG+TGG 0.666361 contig406end:-106882 None:intergenic
GATGTTGATTAGCATAGTAG+GGG 0.671724 contig406end:-106661 None:intergenic
GCTACCTGGATGAAGAGGCG+GGG 0.673729 contig406end:-106771 None:intergenic
AGGGAACATATGTTGAACAA+GGG 0.680887 contig406end:-106700 None:intergenic
GCTCATCAAGTAGTGTAGTG+GGG 0.712201 contig406end:+107129 MsG0080048707.01.T01:CDS
TCTTCAAGTTGAGACCAGTG+TGG 0.762333 contig406end:+107451 MsG0080048707.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTATTTTGTGTTTT+TGG + contig406end:105819-105838 MsG0080048707.01.T01:intron 10.0%
!!! GTTATATATAACATTTTAAA+AGG - contig406end:108030-108049 None:intergenic 10.0%
!! AAAAAAAGAAAAGAAAATGA+TGG + contig406end:108366-108385 MsG0080048707.01.T01:three_prime_UTR 15.0%
!!! ATTTTGTGCAAAATATTATA+TGG + contig406end:108300-108319 MsG0080048707.01.T01:three_prime_UTR 15.0%
!! CAATTCTAAATCAATTTAGT+GGG + contig406end:108075-108094 MsG0080048707.01.T01:three_prime_UTR 20.0%
!!! ATTTTAAAAAGAGAAATGCT+AGG + contig406end:107967-107986 MsG0080048707.01.T01:three_prime_UTR 20.0%
!!! ATTTTGGTTGTTATTATTGA+AGG + contig406end:105773-105792 MsG0080048707.01.T01:intron 20.0%
!!! CTTTCTTTTTTTGATCATTT+TGG + contig406end:105757-105776 MsG0080048707.01.T01:intron 20.0%
!!! TTTTCAACTAACCTATAAAA+AGG - contig406end:108135-108154 None:intergenic 20.0%
! AATACATCTACTCTAGATAA+TGG + contig406end:107647-107666 MsG0080048707.01.T01:three_prime_UTR 25.0%
! CATTTGTATCTACAATTGAT+AGG - contig406end:108274-108293 None:intergenic 25.0%
! GCAATTCTAAATCAATTTAG+TGG + contig406end:108074-108093 MsG0080048707.01.T01:three_prime_UTR 25.0%
! TATCAGCAAAACATAAAAAG+TGG + contig406end:108211-108230 MsG0080048707.01.T01:three_prime_UTR 25.0%
! TATTGCATTTGTGTTTACAA+TGG + contig406end:107904-107923 MsG0080048707.01.T01:three_prime_UTR 25.0%
! TGTTGTTCAACAATTGTATT+CGG - contig406end:106734-106753 None:intergenic 25.0%
! TTGAATTTGAATGATTCGTA+TGG + contig406end:108445-108464 MsG0080048707.01.T01:three_prime_UTR 25.0%
!! ACTATCATCAACCTTTTTAT+AGG + contig406end:108121-108140 MsG0080048707.01.T01:three_prime_UTR 25.0%
!! ATATTGCTGATGATAATGTA+CGG - contig406end:108399-108418 None:intergenic 25.0%
!! TATTTCATCTAGTCTTTGTT+TGG + contig406end:107529-107548 MsG0080048707.01.T01:three_prime_UTR 25.0%
!! TTATCTAGAGTAGATGTATT+TGG - contig406end:107647-107666 None:intergenic 25.0%
!!! ATTAAAGCTGTTTTTGTTGT+CGG + contig406end:106988-107007 MsG0080048707.01.T01:intron 25.0%
!!! CTTACTAGATTTTTTTTGTG+CGG - contig406end:107033-107052 None:intergenic 25.0%
!!! TATATGGCGCAATTTTAATT+TGG + contig406end:108316-108335 MsG0080048707.01.T01:three_prime_UTR 25.0%
AAATGATCTCAGAGAAGAAA+AGG + contig406end:106472-106491 MsG0080048707.01.T01:CDS 30.0%
AAGACTAGATGAAATAAGCA+TGG - contig406end:107525-107544 None:intergenic 30.0%
AATTGTATTCGGAGCTAAAA+AGG - contig406end:106723-106742 None:intergenic 30.0%
ACATACACTTCAATGTGTAT+GGG - contig406end:107732-107751 None:intergenic 30.0%
ATTGTATTCGGAGCTAAAAA+GGG - contig406end:106722-106741 None:intergenic 30.0%
CTAGAATTCATTCATGTGTT+AGG + contig406end:106136-106155 MsG0080048707.01.T01:intron 30.0%
GTTTACTAACTTGAGAAGTA+AGG - contig406end:106308-106327 None:intergenic 30.0%
TAATCACAAACTCTCCTAAA+AGG + contig406end:107498-107517 MsG0080048707.01.T01:three_prime_UTR 30.0%
TATTGTTGTGTTCTCTTTGT+TGG + contig406end:108175-108194 MsG0080048707.01.T01:three_prime_UTR 30.0%
TGACAAATTGAAATTGCAGA+TGG + contig406end:107424-107443 MsG0080048707.01.T01:three_prime_UTR 30.0%
TGAGAGCAAAGAATTATCAT+TGG - contig406end:108547-108566 None:intergenic 30.0%
TGCAATTTCAATTTGTCACA+AGG - contig406end:107422-107441 None:intergenic 30.0%
TTAGGAGAGTTTGTGATTAA+GGG - contig406end:107497-107516 None:intergenic 30.0%
TTCAAAGTCGAGAAAAAGAA+AGG - contig406end:108509-108528 None:intergenic 30.0%
TTCAATTTGTCACAAGGAAT+AGG - contig406end:107416-107435 None:intergenic 30.0%
TTTAGGAGAGTTTGTGATTA+AGG - contig406end:107498-107517 None:intergenic 30.0%
! ATGAATTACCTTGATGCTTT+TGG + contig406end:106390-106409 MsG0080048707.01.T01:intron 30.0%
! TGAATTACCTTGATGCTTTT+GGG + contig406end:106391-106410 MsG0080048707.01.T01:intron 30.0%
! TTTGGGACATAAATTTACAG+AGG + contig406end:106408-106427 MsG0080048707.01.T01:intron 30.0%
! TTTTATGTGTTTACTGCGAA+AGG + contig406end:105843-105862 MsG0080048707.01.T01:intron 30.0%
!! ACTTTGAAGTGTGTTGAATA+AGG + contig406end:108521-108540 MsG0080048707.01.T01:three_prime_UTR 30.0%
!! ATGGTCGCTTTATCATTTTT+AGG - contig406end:106255-106274 None:intergenic 30.0%
!! ATTTAGTGGGTTATAGTTCT+TGG + contig406end:108088-108107 MsG0080048707.01.T01:three_prime_UTR 30.0%
!! CTACTCTAGATAATGGATAA+TGG + contig406end:107654-107673 MsG0080048707.01.T01:three_prime_UTR 30.0%
!! TATCATTTTTAGGCCTATCA+GGG - contig406end:106245-106264 None:intergenic 30.0%
!! TTATCATTTTTAGGCCTATC+AGG - contig406end:106246-106265 None:intergenic 30.0%
!!! AAACTTTTCTTTGAGTTGTG+AGG + contig406end:107358-107377 MsG0080048707.01.T01:three_prime_UTR 30.0%
!!! AACTTTTCTTTGAGTTGTGA+GGG + contig406end:107359-107378 MsG0080048707.01.T01:three_prime_UTR 30.0%
!!! AATGAACAGTTTTTAGAGCT+GGG + contig406end:105949-105968 MsG0080048707.01.T01:CDS 30.0%
!!! TTTTTTGGACGAGAATAGAA+GGG - contig406end:107271-107290 None:intergenic 30.0%
AAAAATCTAGTAAGTCGTCG+AGG + contig406end:107039-107058 MsG0080048707.01.T01:CDS 35.0%
AACTAAGGACTATGTATCCA+TGG + contig406end:106543-106562 MsG0080048707.01.T01:CDS 35.0%
AAGGGAACATATGTTGAACA+AGG - contig406end:106704-106723 None:intergenic 35.0%
AATGATAAAGCGACCATTCT+AGG + contig406end:106258-106277 MsG0080048707.01.T01:CDS 35.0%
AATGGATCATTCCGTTATGA+TGG + contig406end:106571-106590 MsG0080048707.01.T01:CDS 35.0%
ACACATTGAAGTGTATGTGA+GGG + contig406end:107734-107753 MsG0080048707.01.T01:three_prime_UTR 35.0%
ACATATGTTGAACAAGGGTT+AGG - contig406end:106698-106717 None:intergenic 35.0%
ACTCCAAAAACAAATCATCC+AGG + contig406end:106819-106838 MsG0080048707.01.T01:CDS 35.0%
AGATACAGTTCAATTGCTGA+AGG + contig406end:106281-106300 MsG0080048707.01.T01:CDS 35.0%
AGGGAACATATGTTGAACAA+GGG - contig406end:106703-106722 None:intergenic 35.0%
ATAACTCAAATCTTGTGCGA+GGG + contig406end:108045-108064 MsG0080048707.01.T01:three_prime_UTR 35.0%
CAAGACAAGACTATAATCAC+AGG - contig406end:107795-107814 None:intergenic 35.0%
CACATACACTTCAATGTGTA+TGG - contig406end:107733-107752 None:intergenic 35.0%
CATACACTTCAATGTGTATG+GGG - contig406end:107731-107750 None:intergenic 35.0%
CATATGTTGAACAAGGGTTA+GGG - contig406end:106697-106716 None:intergenic 35.0%
CTCCAAAAACAAATCATCCA+GGG + contig406end:106820-106839 MsG0080048707.01.T01:CDS 35.0%
GTACTATGGTCGAAAAACTT+GGG + contig406end:107764-107783 MsG0080048707.01.T01:three_prime_UTR 35.0%
TACACATTGAAGTGTATGTG+AGG + contig406end:107733-107752 MsG0080048707.01.T01:three_prime_UTR 35.0%
TAGCAGGTGTAATTTCCATT+GGG - contig406end:105882-105901 None:intergenic 35.0%
TATAACTCAAATCTTGTGCG+AGG + contig406end:108044-108063 MsG0080048707.01.T01:three_prime_UTR 35.0%
TCTGTTCTCTCTTCTTAACT+AGG + contig406end:107301-107320 MsG0080048707.01.T01:three_prime_UTR 35.0%
TGTACTATGGTCGAAAAACT+TGG + contig406end:107763-107782 MsG0080048707.01.T01:three_prime_UTR 35.0%
TTAAGAAGAGAGAACAGAAG+CGG - contig406end:107299-107318 None:intergenic 35.0%
TTGAACTGTATCTCCTAGAA+TGG - contig406end:106274-106293 None:intergenic 35.0%
! AGGATGTTGATTAGCATAGT+AGG - contig406end:106666-106685 None:intergenic 35.0%
! CGTGAGGTAATATTTTCTCT+CGG + contig406end:106363-106382 MsG0080048707.01.T01:intron 35.0%
! GAAATAAGCATGGTCCTTTT+AGG - contig406end:107515-107534 None:intergenic 35.0%
! GATGTTGATTAGCATAGTAG+GGG - contig406end:106664-106683 None:intergenic 35.0%
! GGATGTTGATTAGCATAGTA+GGG - contig406end:106665-106684 None:intergenic 35.0%
! TCAATGTGTATGGGGTTTTA+GGG - contig406end:107723-107742 None:intergenic 35.0%
! TTAGAGCTGGGTAACATTTT+AGG + contig406end:105961-105980 MsG0080048707.01.T01:intron 35.0%
! TTCAATGTGTATGGGGTTTT+AGG - contig406end:107724-107743 None:intergenic 35.0%
! TTTTCAACTACATGTTGCGA+AGG + contig406end:106064-106083 MsG0080048707.01.T01:intron 35.0%
!! AATTTTCAAGCAGTGGCTTT+TGG + contig406end:105687-105706 MsG0080048707.01.T01:CDS 35.0%
!! AGGAACAAATTTTCAAGCAG+TGG + contig406end:105680-105699 MsG0080048707.01.T01:CDS 35.0%
!! TTTATGTCCCAAAAGCATCA+AGG - contig406end:106401-106420 None:intergenic 35.0%
!! TTTTTGTTGTCGGATTTTGC+AGG + contig406end:106998-107017 MsG0080048707.01.T01:intron 35.0%
!!! CAATGAACAGTTTTTAGAGC+TGG + contig406end:105948-105967 MsG0080048707.01.T01:CDS 35.0%
!!! CTTTTTTGGACGAGAATAGA+AGG - contig406end:107272-107291 None:intergenic 35.0%
!!! GTTAGTGCTGCTTTTTTCTT+GGG + contig406end:106926-106945 MsG0080048707.01.T01:intron 35.0%
!!! TTACATGCGAAGAGTGTTTT+TGG - contig406end:107946-107965 None:intergenic 35.0%
AAACCTACATTTAGCCACAC+TGG - contig406end:107468-107487 None:intergenic 40.0%
AAGGAAACCAGTTTCACAGA+AGG + contig406end:107062-107081 MsG0080048707.01.T01:CDS 40.0%
ACCTATAGATGCAGTAGAAG+AGG + contig406end:106959-106978 MsG0080048707.01.T01:intron 40.0%
ACCTCTTCTACTGCATCTAT+AGG - contig406end:106963-106982 None:intergenic 40.0%
ACCTGCTAGAAAGACACAAA+AGG + contig406end:105894-105913 MsG0080048707.01.T01:intron 40.0%
AGAGCACAGAGTAATTCAAG+AGG - contig406end:106201-106220 None:intergenic 40.0%
AGCAGGTGTAATTTCCATTG+GGG - contig406end:105881-105900 None:intergenic 40.0%
AGCTCATCAAGTAGTGTAGT+GGG + contig406end:107128-107147 MsG0080048707.01.T01:CDS 40.0%
CTAGCAGGTGTAATTTCCAT+TGG - contig406end:105883-105902 None:intergenic 40.0%
GAAAATGTTGAACGTACGTC+TGG + contig406end:107561-107580 MsG0080048707.01.T01:three_prime_UTR 40.0%
GAAAGGCTAGTGAATGAGAA+TGG + contig406end:107156-107175 MsG0080048707.01.T01:exon 40.0%
GCTAAATGAAGAATCTCGTG+AGG + contig406end:106347-106366 MsG0080048707.01.T01:CDS 40.0%
GTATGGTTGCATCAAAAGCA+TGG + contig406end:108462-108481 MsG0080048707.01.T01:three_prime_UTR 40.0%
GTGAGGGATATGATGTACTA+TGG + contig406end:107750-107769 MsG0080048707.01.T01:three_prime_UTR 40.0%
GTTCAATTTCCAGTTGTCTC+AGG + contig406end:106445-106464 MsG0080048707.01.T01:intron 40.0%
TAGCTCATCAAGTAGTGTAG+TGG + contig406end:107127-107146 MsG0080048707.01.T01:CDS 40.0%
TGACCGAGAAAAGTTAAGGA+GGG + contig406end:105918-105937 MsG0080048707.01.T01:CDS 40.0%
TGATTAGCATAGTAGGGGTA+CGG - contig406end:106659-106678 None:intergenic 40.0%
TTCACAGAAGGTAGTTCGTT+AGG + contig406end:107074-107093 MsG0080048707.01.T01:CDS 40.0%
TTCTGCTGCAACTATTGTAG+AGG + contig406end:107238-107257 MsG0080048707.01.T01:three_prime_UTR 40.0%
TTCTTCATCTCACAGTGTTC+AGG + contig406end:107103-107122 MsG0080048707.01.T01:CDS 40.0%
TTGAATTACTCTGTGCTCTG+TGG + contig406end:106202-106221 MsG0080048707.01.T01:intron 40.0%
! ATAACGGAATGATCCATTGC+AGG - contig406end:106569-106588 None:intergenic 40.0%
! CATTTTTCTCCTGAGACAAC+TGG - contig406end:106457-106476 None:intergenic 40.0%
! CTACTGTGAGGGTTTTCATT+GGG + contig406end:107214-107233 MsG0080048707.01.T01:three_prime_UTR 40.0%
! GATCCCTCCTTAACTTTTCT+CGG - contig406end:105924-105943 None:intergenic 40.0%
!! TTCTTTGAGTTGTGAGGGAA+AGG + contig406end:107364-107383 MsG0080048707.01.T01:three_prime_UTR 40.0%
!!! ACAGAAGCGGCTTTCTTTTT+TGG - contig406end:107286-107305 None:intergenic 40.0%
!!! AGTCTTGTTTTCCTGATACG+TGG - contig406end:106802-106821 None:intergenic 40.0%
!!! CTCCCTGGATGATTTGTTTT+TGG - contig406end:106825-106844 None:intergenic 40.0%
!!! GGTTAGTGCTGCTTTTTTCT+TGG + contig406end:106925-106944 MsG0080048707.01.T01:intron 40.0%
!!! GTCTTGTTTTCCTGATACGT+GGG - contig406end:106801-106820 None:intergenic 40.0%
ACCAGTATCAGCTGCAACTA+AGG + contig406end:106528-106547 MsG0080048707.01.T01:CDS 45.0%
ACCCTCACAGTAGCTTCAAA+AGG - contig406end:107207-107226 None:intergenic 45.0%
ACTGGATACGGATATGTTGG+TGG - contig406end:106599-106618 None:intergenic 45.0%
AGACCAGTGTGGCTAAATGT+AGG + contig406end:107462-107481 MsG0080048707.01.T01:three_prime_UTR 45.0%
AGGCTGACCGAGAAAAGTTA+AGG + contig406end:105914-105933 MsG0080048707.01.T01:CDS 45.0%
AGTCTTCAACTCAAGGTCTG+TGG - contig406end:106885-106904 None:intergenic 45.0%
ATCTAGTAAGTCGTCGAGGA+AGG + contig406end:107043-107062 MsG0080048707.01.T01:CDS 45.0%
CAAAATGTGAGCAGAGTCAC+TGG + contig406end:106019-106038 MsG0080048707.01.T01:intron 45.0%
CGAACTACCTTCTGTGAAAC+TGG - contig406end:107072-107091 None:intergenic 45.0%
CTGACCGAGAAAAGTTAAGG+AGG + contig406end:105917-105936 MsG0080048707.01.T01:CDS 45.0%
GAGCAAGGCTCAAAGAAATG+AGG + contig406end:106844-106863 MsG0080048707.01.T01:CDS 45.0%
GCTCATCAAGTAGTGTAGTG+GGG + contig406end:107129-107148 MsG0080048707.01.T01:CDS 45.0%
GGAACTGGATACGGATATGT+TGG - contig406end:106602-106621 None:intergenic 45.0%
TATGTATCCATGGCCTGCAA+TGG + contig406end:106553-106572 MsG0080048707.01.T01:CDS 45.0%
TCCTTAGTTGCAGCTGATAC+TGG - contig406end:106532-106551 None:intergenic 45.0%
! AAGCATGGTCTTGACTGATG+AGG + contig406end:108477-108496 MsG0080048707.01.T01:three_prime_UTR 45.0%
! AGCATGGTCTTGACTGATGA+GGG + contig406end:108478-108497 MsG0080048707.01.T01:three_prime_UTR 45.0%
! GCTACTGTGAGGGTTTTCAT+TGG + contig406end:107213-107232 MsG0080048707.01.T01:three_prime_UTR 45.0%
! GTGGCTTTTGGACATGTCAA+AGG + contig406end:105699-105718 MsG0080048707.01.T01:CDS 45.0%
! TCTGAAATGCAGACCCTGAT+AGG + contig406end:106229-106248 MsG0080048707.01.T01:intron 45.0%
! TGTTGGTGGAGCCATCATAA+CGG - contig406end:106585-106604 None:intergenic 45.0%
!! ACCTTGAGTTGAAGACTCCT+GGG + contig406end:106888-106907 MsG0080048707.01.T01:CDS 45.0%
!! GCCTTTTGTGTCTTTCTAGC+AGG - contig406end:105898-105917 None:intergenic 45.0%
!! TCTTCAAGTTGAGACCAGTG+TGG + contig406end:107451-107470 MsG0080048707.01.T01:three_prime_UTR 45.0%
!!! GCCTTTTGAAGCTACTGTGA+GGG + contig406end:107203-107222 MsG0080048707.01.T01:three_prime_UTR 45.0%
!!! TGCCTTTTGAAGCTACTGTG+AGG + contig406end:107202-107221 MsG0080048707.01.T01:three_prime_UTR 45.0%
!! TTTAACAAATTAAAAAAATA+AGG + contig406end:107849-107868 MsG0080048707.01.T01:three_prime_UTR 5.0%
ACCCAGGAGTCTTCAACTCA+AGG - contig406end:106892-106911 None:intergenic 50.0%
AGCAGCACTAACCTGATCTG+CGG - contig406end:106918-106937 None:intergenic 50.0%
CAAATCATCCAGGGAGAGCA+AGG + contig406end:106829-106848 MsG0080048707.01.T01:CDS 50.0%
GAATGATCCATTGCAGGCCA+TGG - contig406end:106563-106582 None:intergenic 50.0%
GGAATTGAACCAGCAGGTAC+CGG - contig406end:106629-106648 None:intergenic 50.0%
GTTGAACGTACGTCTGGACT+CGG + contig406end:107567-107586 MsG0080048707.01.T01:three_prime_UTR 50.0%
TCCGTATCCAGTTCCAATGC+CGG + contig406end:106607-106626 MsG0080048707.01.T01:CDS 50.0%
TGCATGGGAATTGAACCAGC+AGG - contig406end:106635-106654 None:intergenic 50.0%
! ACCGGCATTGGAACTGGATA+CGG - contig406end:106611-106630 None:intergenic 50.0%
! GGTCTGAAGTTGAACCCCAA+TGG + contig406end:105864-105883 MsG0080048707.01.T01:intron 50.0%
! GTTTTCCTGATACGTGGGAC+CGG - contig406end:106796-106815 None:intergenic 50.0%
! TAGTGTAGTGGGGAGCAGAA+AGG + contig406end:107139-107158 MsG0080048707.01.T01:CDS 50.0%
!! GACCTTGAGTTGAAGACTCC+TGG + contig406end:106887-106906 MsG0080048707.01.T01:CDS 50.0%
!!! GAAGAGTGTTTTTGGCGTGC+TGG - contig406end:107938-107957 None:intergenic 50.0%
AGGCGGGGATACGTATTGAG+TGG - contig406end:106759-106778 None:intergenic 55.0%
CAAGGGTTAGGGATGACTGC+AGG - contig406end:106686-106705 None:intergenic 55.0%
TCTTTGAGCCTTGCTCTCCC+TGG - contig406end:106840-106859 None:intergenic 55.0%
! ATAGTAGGGGTACGGCTGCA+TGG - contig406end:106651-106670 None:intergenic 55.0%
! TAGTAGGGGTACGGCTGCAT+GGG - contig406end:106650-106669 None:intergenic 55.0%
ACCTGATCTGCGGAAGACCC+AGG - contig406end:106908-106927 None:intergenic 60.0%
CGCCTCTTCATCCAGGTAGC+CGG + contig406end:106774-106793 MsG0080048707.01.T01:CDS 60.0%
CGGCTACCTGGATGAAGAGG+CGG - contig406end:106776-106795 None:intergenic 60.0%
GACCGGCTACCTGGATGAAG+AGG - contig406end:106779-106798 None:intergenic 60.0%
GCTACCTGGATGAAGAGGCG+GGG - contig406end:106774-106793 None:intergenic 60.0%
GGCTACCTGGATGAAGAGGC+GGG - contig406end:106775-106794 None:intergenic 60.0%
GTATCCCCGCCTCTTCATCC+AGG + contig406end:106767-106786 MsG0080048707.01.T01:CDS 60.0%
GTTCCAATGCCGGTACCTGC+TGG + contig406end:106617-106636 MsG0080048707.01.T01:CDS 60.0%
TCCTGGGTCTTCCGCAGATC+AGG + contig406end:106904-106923 MsG0080048707.01.T01:CDS 60.0%
TGTGAGGGAAAGGCTGCGTG+AGG + contig406end:107374-107393 MsG0080048707.01.T01:three_prime_UTR 60.0%
!! GAACCAGCAGGTACCGGCAT+TGG - contig406end:106623-106642 None:intergenic 60.0%
!! GCAGGTACCGGCATTGGAAC+TGG - contig406end:106617-106636 None:intergenic 60.0%
GATACGTGGGACCGGCTACC+TGG - contig406end:106788-106807 None:intergenic 65.0%
GGTAGCCGGTCCCACGTATC+AGG + contig406end:106788-106807 MsG0080048707.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
contig406end gene 105674 108600 105674 ID=MsG0080048707.01;Name=MsG0080048707.01
contig406end mRNA 105674 108600 105674 ID=MsG0080048707.01.T01;Parent=MsG0080048707.01;Name=MsG0080048707.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0.25|0.4|1|0.75|0.6|5|1428|291
contig406end exon 105674 105741 105674 ID=MsG0080048707.01.T01:exon:20882;Parent=MsG0080048707.01.T01
contig406end exon 105903 105970 105903 ID=MsG0080048707.01.T01:exon:20883;Parent=MsG0080048707.01.T01
contig406end exon 106241 106368 106241 ID=MsG0080048707.01.T01:exon:20884;Parent=MsG0080048707.01.T01
contig406end exon 106467 106925 106467 ID=MsG0080048707.01.T01:exon:20885;Parent=MsG0080048707.01.T01
contig406end exon 107020 108600 107020 ID=MsG0080048707.01.T01:exon:20886;Parent=MsG0080048707.01.T01
contig406end CDS 105674 105741 105674 ID=MsG0080048707.01.T01:cds;Parent=MsG0080048707.01.T01
contig406end CDS 105903 105970 105903 ID=MsG0080048707.01.T01:cds;Parent=MsG0080048707.01.T01
contig406end CDS 106241 106368 106241 ID=MsG0080048707.01.T01:cds;Parent=MsG0080048707.01.T01
contig406end CDS 106467 106925 106467 ID=MsG0080048707.01.T01:cds;Parent=MsG0080048707.01.T01
contig406end CDS 107020 107172 107020 ID=MsG0080048707.01.T01:cds;Parent=MsG0080048707.01.T01
contig406end three_prime_UTR 107173 108600 107173 ID=MsG0080048707.01.T01:three_prime_utr;Parent=MsG0080048707.01.T01
Gene Sequence

>MsG0080048707.01.T01

ATGTGTAGGAACAAATTTTCAAGCAGTGGCTTTTGGACATGTCAAAGGTCACTGCTTGAAGAGTTGATAAAGACACAAAAGGCTGACCGAGAAAAGTTAAGGAGGGATCGACTCAATGAACAGTTTTTAGAGCTGGACCCTGATAGGCCTAAAAATGATAAAGCGACCATTCTAGGAGATACAGTTCAATTGCTGAAGGACCTTACTTCTCAAGTTAGTAAACTAAAAGATGAATATACTCTGCTAAATGAAGAATCTCGTGAGGAGAAAAATGATCTCAGAGAAGAAAAGGCTTCTCTTAAATCAGATATTGAGAATTTGAATAACCAGTATCAGCTGCAACTAAGGACTATGTATCCATGGCCTGCAATGGATCATTCCGTTATGATGGCTCCACCAACATATCCGTATCCAGTTCCAATGCCGGTACCTGCTGGTTCAATTCCCATGCAGCCGTACCCCTACTATGCTAATCAACATCCTGCAGTCATCCCTAACCCTTGTTCAACATATGTTCCCTTTTTAGCTCCGAATACAATTGTTGAACAACAATCCACTCAATACGTATCCCCGCCTCTTCATCCAGGTAGCCGGTCCCACGTATCAGGAAAACAAGACTCCAAAAACAAATCATCCAGGGAGAGCAAGGCTCAAAGAAATGAGGATTCTAATGATGTAGCCACAGACCTTGAGTTGAAGACTCCTGGGTCTTCCGCAGATCAGGATTTAACATCCGCACAAAAAAAATCTAGTAAGTCGTCGAGGAAGGAAACCAGTTTCACAGAAGGTAGTTCGTTAGGCAAGTGTTCTTCATCTCACAGTGTTCAGGATAGCTCATCAAGTAGTGTAGTGGGGAGCAGAAAGGCTAGTGAATGA
Protein sequence

>MsG0080048707.01.T01

MCRNKFSSSGFWTCQRSLLEELIKTQKADREKLRRDRLNEQFLELDPDRPKNDKATILGDTVQLLKDLTSQVSKLKDEYTLLNEESREEKNDLREEKASLKSDIENLNNQYQLQLRTMYPWPAMDHSVMMAPPTYPYPVPMPVPAGSIPMQPYPYYANQHPAVIPNPCSTYVPFLAPNTIVEQQSTQYVSPPLHPGSRSHVSGKQDSKNKSSRESKAQRNEDSNDVATDLELKTPGSSADQDLTSAQKKSSKSSRKETSFTEGSSLGKCSSSHSVQDSSSSSVVGSRKASE*