Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048875.01.T01 | XP_039685407.1 | 96.175 | 366 | 14 | 0 | 1 | 366 | 36 | 401 | 0 | 731 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048875.01.T01 | Q9SJ74 | 69.919 | 369 | 106 | 4 | 1 | 366 | 38 | 404 | 0 | 519 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048875.01.T01 | A0A072TKQ9 | 96.175 | 366 | 14 | 0 | 1 | 366 | 36 | 401 | 0.0 | 731 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0080048875.01 | MsG0280007481.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048875.01.T01 | MTR_8g009880 | 96.175 | 366 | 14 | 0 | 1 | 366 | 36 | 401 | 0.0 | 731 |
| MsG0080048875.01.T01 | MTR_5g097620 | 85.095 | 369 | 51 | 3 | 1 | 365 | 73 | 441 | 0.0 | 639 |
| MsG0080048875.01.T01 | MTR_4g133620 | 34.309 | 376 | 213 | 9 | 2 | 362 | 41 | 397 | 1.07e-63 | 208 |
| MsG0080048875.01.T01 | MTR_2g026080 | 36.043 | 369 | 210 | 10 | 2 | 360 | 45 | 397 | 3.56e-63 | 207 |
| MsG0080048875.01.T01 | MTR_7g051910 | 32.527 | 372 | 226 | 6 | 1 | 365 | 40 | 393 | 1.72e-61 | 202 |
| MsG0080048875.01.T01 | MTR_5g093520 | 35.244 | 349 | 200 | 9 | 3 | 339 | 54 | 388 | 8.31e-59 | 196 |
| MsG0080048875.01.T01 | MTR_3g058510 | 30.595 | 353 | 220 | 8 | 2 | 345 | 42 | 378 | 7.13e-52 | 177 |
| MsG0080048875.01.T01 | MTR_8g028075 | 31.858 | 226 | 144 | 7 | 113 | 336 | 336 | 553 | 1.18e-30 | 122 |
| MsG0080048875.01.T01 | MTR_7g117375 | 31.399 | 293 | 166 | 13 | 34 | 317 | 92 | 358 | 2.17e-21 | 95.1 |
| MsG0080048875.01.T01 | MTR_2g018960 | 29.814 | 161 | 103 | 3 | 153 | 307 | 651 | 807 | 4.63e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048875.01.T01 | AT2G04850 | 69.919 | 369 | 106 | 4 | 1 | 366 | 38 | 404 | 0.0 | 519 |
| MsG0080048875.01.T01 | AT4G12980 | 34.444 | 360 | 212 | 5 | 1 | 349 | 44 | 390 | 9.00e-70 | 224 |
| MsG0080048875.01.T01 | AT3G25290 | 33.983 | 359 | 215 | 5 | 1 | 349 | 43 | 389 | 1.65e-69 | 223 |
| MsG0080048875.01.T01 | AT3G25290 | 33.983 | 359 | 215 | 5 | 1 | 349 | 43 | 389 | 1.65e-69 | 223 |
| MsG0080048875.01.T01 | AT5G47530 | 33.803 | 355 | 213 | 8 | 2 | 350 | 43 | 381 | 2.75e-66 | 215 |
| MsG0080048875.01.T01 | AT5G35735 | 33.705 | 359 | 212 | 10 | 12 | 365 | 55 | 392 | 7.76e-62 | 204 |
| MsG0080048875.01.T01 | AT4G17280 | 35.097 | 359 | 209 | 9 | 2 | 352 | 50 | 392 | 5.83e-61 | 201 |
| MsG0080048875.01.T01 | AT3G59070 | 30.532 | 357 | 214 | 12 | 21 | 361 | 72 | 410 | 5.66e-46 | 163 |
| MsG0080048875.01.T01 | AT3G07570 | 27.966 | 354 | 209 | 12 | 2 | 337 | 42 | 367 | 5.20e-30 | 118 |
| MsG0080048875.01.T01 | AT5G48750 | 29.954 | 217 | 134 | 4 | 1 | 213 | 49 | 251 | 6.14e-28 | 110 |
| MsG0080048875.01.T01 | AT3G61750 | 28.053 | 303 | 196 | 10 | 33 | 333 | 11 | 293 | 1.11e-26 | 108 |
| MsG0080048875.01.T01 | AT3G61750 | 27.103 | 321 | 212 | 10 | 15 | 333 | 70 | 370 | 3.22e-26 | 108 |
| MsG0080048875.01.T01 | AT5G54830 | 29.412 | 187 | 111 | 6 | 160 | 340 | 670 | 841 | 5.15e-14 | 73.9 |
Find 130 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTGTTTGCTTTCATTCT+TGG | 0.155320 | contig503end:+7325 | MsG0080048875.01.T01:CDS |
| ATTCCAAACAAGGTGGATTT+TGG | 0.189165 | contig503end:-7044 | MsG0080048875.01.T01:intergenic |
| GTTTGATTCTAGTTTGAATT+TGG | 0.197666 | contig503end:-7017 | MsG0080048875.01.T01:intergenic |
| GGTACTTTCATTTCACCTTC+TGG | 0.249020 | contig503end:+6749 | MsG0080048875.01.T01:CDS |
| AGACTTTAGCATTGTTGTTT+AGG | 0.279742 | contig503end:+7455 | MsG0080048875.01.T01:CDS |
| ACCTTAGAGCTAGTCTTCTT+TGG | 0.283155 | contig503end:+6728 | MsG0080048875.01.T01:CDS |
| AAACCAAAATCCACCTTGTT+TGG | 0.304424 | contig503end:+7041 | MsG0080048875.01.T01:CDS |
| GATTGGTGTTTCCATAGCTT+TGG | 0.318729 | contig503end:+7642 | MsG0080048875.01.T01:CDS |
| CACCTTCTGGATGGGTTGGA+TGG | 0.322109 | contig503end:+6762 | MsG0080048875.01.T01:CDS |
| CGATAACTGCACGTTACTTT+AGG | 0.338646 | contig503end:+7254 | MsG0080048875.01.T01:CDS |
| TCCTGTCATCTCCGATGAAA+TGG | 0.341469 | contig503end:-6795 | MsG0080048875.01.T01:intergenic |
| GTTTGAATTTGGCATAGATT+TGG | 0.341934 | contig503end:-7006 | MsG0080048875.01.T01:intergenic |
| CTGAACTTGTTCCTAGCGTT+TGG | 0.344648 | contig503end:-7478 | MsG0080048875.01.T01:intergenic |
| TTCGCCATTGGCATTCGCCT+TGG | 0.354937 | contig503end:+7358 | MsG0080048875.01.T01:CDS |
| GCTTTCATTCTTGGAACTGT+GGG | 0.358819 | contig503end:+7334 | MsG0080048875.01.T01:CDS |
| AATCCTACCACCACTTTGTT+GGG | 0.359563 | contig503end:+7512 | MsG0080048875.01.T01:CDS |
| TGCCAAGCTGAGTTACTGTT+TGG | 0.360154 | contig503end:+7606 | MsG0080048875.01.T01:CDS |
| ACCTTCTGGATGGGTTGGAT+GGG | 0.360379 | contig503end:+6763 | MsG0080048875.01.T01:CDS |
| TCTTCCATGGCGGCCATATA+TGG | 0.369335 | contig503end:+6947 | MsG0080048875.01.T01:CDS |
| GGCCATATATGGTGGCAAAA+AGG | 0.375935 | contig503end:+6958 | MsG0080048875.01.T01:CDS |
| TCGCCATTGGCATTCGCCTT+GGG | 0.378753 | contig503end:+7359 | MsG0080048875.01.T01:CDS |
| TTGGGACTTTCTACTCTGAT+TGG | 0.385534 | contig503end:+7625 | MsG0080048875.01.T01:CDS |
| CCTCTCTTCCATTGCCACCT+TGG | 0.387175 | contig503end:+7123 | MsG0080048875.01.T01:CDS |
| GATATTCTGTAGGAAATCTA+AGG | 0.396344 | contig503end:+7681 | MsG0080048875.01.T01:CDS |
| AAATCCTACCACCACTTTGT+TGG | 0.398807 | contig503end:+7511 | MsG0080048875.01.T01:CDS |
| GCCACCTTGGACGTGTTGTC+AGG | 0.406139 | contig503end:+7136 | MsG0080048875.01.T01:CDS |
| ACCAAAGAAGACTAGCTCTA+AGG | 0.410370 | contig503end:-6729 | MsG0080048875.01.T01:intergenic |
| GGGTACTCTTGTGTGGTGCT+CGG | 0.419514 | contig503end:+7532 | MsG0080048875.01.T01:CDS |
| TCCATGCTATAGAGGCTTGT+TGG | 0.422158 | contig503end:-6685 | MsG0080048875.01.T01:intergenic |
| TTTCAAGGGTTTGAAGTGAT+GGG | 0.426534 | contig503end:+7568 | MsG0080048875.01.T01:CDS |
| CTTTCATTTCACCTTCTGGA+TGG | 0.427020 | contig503end:+6753 | MsG0080048875.01.T01:CDS |
| GGTACTCTTGTGTGGTGCTC+GGG | 0.429304 | contig503end:+7533 | MsG0080048875.01.T01:CDS |
| ATTTCACCTTCTGGATGGGT+TGG | 0.432760 | contig503end:+6758 | MsG0080048875.01.T01:CDS |
| AATCGAGGACTTTATGTTCA+AGG | 0.432833 | contig503end:+7064 | MsG0080048875.01.T01:CDS |
| GGGTTTGTGAATGTGTTTCA+AGG | 0.433084 | contig503end:+7553 | MsG0080048875.01.T01:CDS |
| CCTTCTGGATGGGTTGGATG+GGG | 0.433473 | contig503end:+6764 | MsG0080048875.01.T01:CDS |
| AGGACTACTTGGCCTGAACT+TGG | 0.435228 | contig503end:-6845 | MsG0080048875.01.T01:intergenic |
| CCATGCTATAGAGGCTTGTT+GGG | 0.439886 | contig503end:-6684 | MsG0080048875.01.T01:intergenic |
| TAGCTTTGGAGGTGAACTCT+TGG | 0.445429 | contig503end:+7656 | MsG0080048875.01.T01:CDS |
| CGCCTTGGGCAATTGTCTCC+AGG | 0.448142 | contig503end:+7373 | MsG0080048875.01.T01:CDS |
| GGAACTGTGGGGTTCGCCAT+TGG | 0.448882 | contig503end:+7346 | MsG0080048875.01.T01:CDS |
| CCTGTCATCTCCGATGAAAT+GGG | 0.449627 | contig503end:-6794 | MsG0080048875.01.T01:intergenic |
| TTTCATTTCACCTTCTGGAT+GGG | 0.456584 | contig503end:+6754 | MsG0080048875.01.T01:CDS |
| GTTTCAAGGGTTTGAAGTGA+TGG | 0.464273 | contig503end:+7567 | MsG0080048875.01.T01:CDS |
| AGCTTTGGAGGTGAACTCTT+GGG | 0.465949 | contig503end:+7657 | MsG0080048875.01.T01:CDS |
| GCCAAGCTGAGTTACTGTTT+GGG | 0.467839 | contig503end:+7607 | MsG0080048875.01.T01:CDS |
| AGCTCTAAGGTGGAGTTATG+AGG | 0.469977 | contig503end:-6716 | MsG0080048875.01.T01:intergenic |
| CAAACGCTAGGAACAAGTTC+AGG | 0.470832 | contig503end:+7479 | MsG0080048875.01.T01:CDS |
| TATAGCCTCCACAGGAAGCT+TGG | 0.472577 | contig503end:+7403 | MsG0080048875.01.T01:CDS |
| TACTTTGAATGCCATCTCAT+GGG | 0.476629 | contig503end:+7210 | MsG0080048875.01.T01:CDS |
| GGAACAAGTTCAGGAAGTAT+TGG | 0.480063 | contig503end:+7488 | MsG0080048875.01.T01:CDS |
| GAGAGGTCAGTGGAGGTGGT+TGG | 0.482995 | contig503end:-7106 | MsG0080048875.01.T01:intergenic |
| CATTCACCTTATCTCTTCCA+TGG | 0.483758 | contig503end:+6934 | MsG0080048875.01.T01:CDS |
| GCTATAGAGGCTTGTTGGGT+GGG | 0.484183 | contig503end:-6680 | MsG0080048875.01.T01:intergenic |
| ACATTCGGGTCTATGATGTA+AGG | 0.484186 | contig503end:-6872 | MsG0080048875.01.T01:intergenic |
| GAATCACTCTCAGCATTGTA+AGG | 0.485398 | contig503end:-7183 | MsG0080048875.01.T01:intergenic |
| GGAAGAGAGGTCAGTGGAGG+TGG | 0.485795 | contig503end:-7110 | MsG0080048875.01.T01:intergenic |
| GAATTTGGCATAGATTTGGA+TGG | 0.486860 | contig503end:-7002 | MsG0080048875.01.T01:intergenic |
| TGCTTTCATTCTTGGAACTG+TGG | 0.487236 | contig503end:+7333 | MsG0080048875.01.T01:CDS |
| ATGAAGAGAGAAGGACTTGT+CGG | 0.488902 | contig503end:+7712 | MsG0080048875.01.T01:CDS |
| GCCTGAACTTGGGTCAGAAA+AGG | 0.492569 | contig503end:-6834 | MsG0080048875.01.T01:intergenic |
| CAGCTTGGCATAGGATCTGC+TGG | 0.493751 | contig503end:-7593 | MsG0080048875.01.T01:intergenic |
| TGTAAGGAAGTAGGACTACT+TGG | 0.498128 | contig503end:-6856 | MsG0080048875.01.T01:intergenic |
| TTCAAGGGTTTGAAGTGATG+GGG | 0.499384 | contig503end:+7569 | MsG0080048875.01.T01:CDS |
| TGGTGTTTCCATAGCTTTGG+AGG | 0.501209 | contig503end:+7645 | MsG0080048875.01.T01:CDS |
| TGGAGGCTATACTCTACTCC+TGG | 0.506212 | contig503end:-7391 | MsG0080048875.01.T01:intergenic |
| GGTCTATGATGTAAGGAAGT+AGG | 0.509135 | contig503end:-6865 | MsG0080048875.01.T01:intergenic |
| AATTTGGCATAGATTTGGAT+GGG | 0.513427 | contig503end:-7001 | MsG0080048875.01.T01:intergenic |
| TGCTATAGAGGCTTGTTGGG+TGG | 0.514341 | contig503end:-6681 | MsG0080048875.01.T01:intergenic |
| GAAGAGAAAATGAAGAGAGA+AGG | 0.521275 | contig503end:+7703 | MsG0080048875.01.T01:CDS |
| AGAGTTCACCTCCAAAGCTA+TGG | 0.533936 | contig503end:-7653 | MsG0080048875.01.T01:intergenic |
| CTAAAGTCTGCAAGGCTCCA+AGG | 0.539524 | contig503end:-7441 | MsG0080048875.01.T01:intergenic |
| GAGGTGGTTGGATGAATAGT+AGG | 0.540410 | contig503end:-7094 | MsG0080048875.01.T01:intergenic |
| GGTTTGTGAATGTGTTTCAA+GGG | 0.541986 | contig503end:+7554 | MsG0080048875.01.T01:CDS |
| ATCCACCTTGTTTGGAATCG+AGG | 0.543575 | contig503end:+7049 | MsG0080048875.01.T01:CDS |
| TTGTCGGAACTTCTGACAAA+GGG | 0.544921 | contig503end:+7728 | MsG0080048875.01.T01:CDS |
| GTACTTTGAATGCCATCTCA+TGG | 0.547445 | contig503end:+7209 | MsG0080048875.01.T01:CDS |
| TCCCAAACAGTAACTCAGCT+TGG | 0.549887 | contig503end:-7608 | MsG0080048875.01.T01:intergenic |
| CCCATTTCATCGGAGATGAC+AGG | 0.549985 | contig503end:+6794 | MsG0080048875.01.T01:CDS |
| CAACACGTCCAAGGTGGCAA+TGG | 0.551704 | contig503end:-7131 | MsG0080048875.01.T01:intergenic |
| ACAGTAACTCAGCTTGGCAT+AGG | 0.553331 | contig503end:-7602 | MsG0080048875.01.T01:intergenic |
| GTAGAAAGTCCATGCTATAG+AGG | 0.558385 | contig503end:-6693 | MsG0080048875.01.T01:intergenic |
| GAGTAGAGTATAGCCTCCAC+AGG | 0.561022 | contig503end:+7395 | MsG0080048875.01.T01:CDS |
| CTTTGTTGGGTACTCTTGTG+TGG | 0.563285 | contig503end:+7525 | MsG0080048875.01.T01:CDS |
| TTGCCCAAGGCGAATGCCAA+TGG | 0.566998 | contig503end:-7362 | MsG0080048875.01.T01:intergenic |
| GCGTGGTTCTATGCTCATGC+AGG | 0.567093 | contig503end:+7298 | MsG0080048875.01.T01:CDS |
| CAACAATGCTAAAGTCTGCA+AGG | 0.567757 | contig503end:-7449 | MsG0080048875.01.T01:intergenic |
| GGGAATCAACCCCATTTCAT+CGG | 0.569185 | contig503end:+6784 | MsG0080048875.01.T01:CDS |
| GCCACCATATATGGCCGCCA+TGG | 0.570636 | contig503end:-6951 | MsG0080048875.01.T01:intergenic |
| TTTAGGCATATTCAATCACT+AGG | 0.571060 | contig503end:+7271 | MsG0080048875.01.T01:CDS |
| CTTTCATTCTTGGAACTGTG+GGG | 0.574407 | contig503end:+7335 | MsG0080048875.01.T01:CDS |
| CGATTCCAAGCTTCCTGTGG+AGG | 0.574797 | contig503end:-7408 | MsG0080048875.01.T01:intergenic |
| GGACTACTTGGCCTGAACTT+GGG | 0.575450 | contig503end:-6844 | MsG0080048875.01.T01:intergenic |
| TGGCCGCCATGGAAGAGATA+AGG | 0.578696 | contig503end:-6940 | MsG0080048875.01.T01:intergenic |
| AAAGTCCTCGATTCCAAACA+AGG | 0.585689 | contig503end:-7054 | MsG0080048875.01.T01:intergenic |
| GCTCTAAGGTGGAGTTATGA+GGG | 0.585753 | contig503end:-6715 | MsG0080048875.01.T01:intergenic |
| TATGTATTCTCTTTCAAGTG+TGG | 0.586082 | contig503end:-7762 | MsG0080048875.01.T01:intergenic |
| TGTTGTTTAGGCCAAACGCT+AGG | 0.586674 | contig503end:+7467 | MsG0080048875.01.T01:CDS |
| ACAATGCTGAGAGTGATTCA+CGG | 0.588148 | contig503end:+7187 | MsG0080048875.01.T01:CDS |
| CCCAACAAGCCTCTATAGCA+TGG | 0.588511 | contig503end:+6684 | MsG0080048875.01.T01:CDS |
| ATTTGGCATAGATTTGGATG+GGG | 0.589019 | contig503end:-7000 | MsG0080048875.01.T01:intergenic |
| CAAAAGAATACCCCATGAGA+TGG | 0.592702 | contig503end:-7221 | MsG0080048875.01.T01:intergenic |
| ACTCTTGGGTGATATTCTGT+AGG | 0.598331 | contig503end:+7671 | MsG0080048875.01.T01:CDS |
| TTTGGCATAGATTTGGATGG+GGG | 0.598707 | contig503end:-6999 | MsG0080048875.01.T01:intergenic |
| TCAAGGGTTTGAAGTGATGG+GGG | 0.598986 | contig503end:+7570 | MsG0080048875.01.T01:CDS |
| AAAGAAGACTAGCTCTAAGG+TGG | 0.603475 | contig503end:-6726 | MsG0080048875.01.T01:intergenic |
| TCAAGTGGTCGAGAGAGTAG+TGG | 0.610217 | contig503end:-6911 | MsG0080048875.01.T01:intergenic |
| CCAAGGTGGCAATGGAAGAG+AGG | 0.618055 | contig503end:-7123 | MsG0080048875.01.T01:intergenic |
| CTGTCATCTCCGATGAAATG+GGG | 0.622824 | contig503end:-6793 | MsG0080048875.01.T01:intergenic |
| CAGCGATTCCAAGCTTCCTG+TGG | 0.623784 | contig503end:-7411 | MsG0080048875.01.T01:intergenic |
| TGAACCTGACAACACGTCCA+AGG | 0.624862 | contig503end:-7140 | MsG0080048875.01.T01:intergenic |
| CCCCATCCAACCCATCCAGA+AGG | 0.626283 | contig503end:-6764 | MsG0080048875.01.T01:intergenic |
| CTTGTCGGAACTTCTGACAA+AGG | 0.629302 | contig503end:+7727 | MsG0080048875.01.T01:CDS |
| GGCAATGGAAGAGAGGTCAG+TGG | 0.631399 | contig503end:-7116 | MsG0080048875.01.T01:intergenic |
| ACTTCTGACAAAGGGAGTAG+TGG | 0.632759 | contig503end:+7736 | MsG0080048875.01.T01:CDS |
| CGTGCAGTTATCGCTCCCAT+CGG | 0.634360 | contig503end:-7244 | MsG0080048875.01.T01:intergenic |
| CACAAGAGTACCCAACAAAG+TGG | 0.636742 | contig503end:-7522 | MsG0080048875.01.T01:intergenic |
| ACTTTGAATGCCATCTCATG+GGG | 0.644712 | contig503end:+7211 | MsG0080048875.01.T01:CDS |
| GTACCCAACAAAGTGGTGGT+AGG | 0.645713 | contig503end:-7515 | MsG0080048875.01.T01:intergenic |
| TCCATGGCGGCCATATATGG+TGG | 0.646343 | contig503end:+6950 | MsG0080048875.01.T01:CDS |
| CTCCTGGAGACAATTGCCCA+AGG | 0.650171 | contig503end:-7375 | MsG0080048875.01.T01:intergenic |
| GAGATAAGGTGAATGTCAAG+TGG | 0.666609 | contig503end:-6926 | MsG0080048875.01.T01:intergenic |
| TTCAATCACTAGGTCCTGCG+TGG | 0.687681 | contig503end:+7281 | MsG0080048875.01.T01:CDS |
| AATGGAAGAGAGGTCAGTGG+AGG | 0.692402 | contig503end:-7113 | MsG0080048875.01.T01:intergenic |
| TCACCTTATCTCTTCCATGG+CGG | 0.701620 | contig503end:+6937 | MsG0080048875.01.T01:CDS |
| GTCCTCGATTCCAAACAAGG+TGG | 0.704268 | contig503end:-7051 | MsG0080048874.01.T01:intergenic |
| AAGAGTACCCAACAAAGTGG+TGG | 0.705072 | contig503end:-7519 | MsG0080048874.01.T01:intergenic |
| GCATGAGCATAGAACCACGC+AGG | 0.706285 | contig503end:-7295 | MsG0080048874.01.T01:intergenic |
| AAGGTCTGTCTGTTGACGAG+CGG | 0.717514 | contig503end:-7164 | MsG0080048874.01.T01:intergenic |
| ACCTGACAACACGTCCAAGG+TGG | 0.719059 | contig503end:-7137 | MsG0080048874.01.T01:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTGATTCTAGTTTGAATT+TGG | - | contig503end:7020-7039 | MsG0080048875.01.T01:intergenic | 25.0% |
| AATTTGGCATAGATTTGGAT+GGG | - | contig503end:7004-7023 | MsG0080048875.01.T01:intergenic | 30.0% | |
| CAATTGTTTGCTTTCATTCT+TGG | + | contig503end:7325-7344 | MsG0080048875.01.T01:CDS | 30.0% | |
| GATATTCTGTAGGAAATCTA+AGG | + | contig503end:7681-7700 | MsG0080048875.01.T01:CDS | 30.0% | |
| GTTTGAATTTGGCATAGATT+TGG | - | contig503end:7009-7028 | MsG0080048875.01.T01:intergenic | 30.0% | |
| TTTAGGCATATTCAATCACT+AGG | + | contig503end:7271-7290 | MsG0080048875.01.T01:CDS | 30.0% | |
| !! | AGACTTTAGCATTGTTGTTT+AGG | + | contig503end:7455-7474 | MsG0080048875.01.T01:CDS | 30.0% |
| !!! | ATTTTTGGAGCTTTACATTC+GGG | - | contig503end:6889-6908 | MsG0080048875.01.T01:intergenic | 30.0% |
| !!! | GATTTTTGGAGCTTTACATT+CGG | - | contig503end:6890-6909 | MsG0080048875.01.T01:intergenic | 30.0% |
| AAACCAAAATCCACCTTGTT+TGG | + | contig503end:7041-7060 | MsG0080048875.01.T01:CDS | 35.0% | |
| AATCGAGGACTTTATGTTCA+AGG | + | contig503end:7064-7083 | MsG0080048875.01.T01:CDS | 35.0% | |
| ATTTGGCATAGATTTGGATG+GGG | - | contig503end:7003-7022 | MsG0080048875.01.T01:intergenic | 35.0% | |
| GAAGAGAAAATGAAGAGAGA+AGG | + | contig503end:7703-7722 | MsG0080048875.01.T01:CDS | 35.0% | |
| GAATTTGGCATAGATTTGGA+TGG | - | contig503end:7005-7024 | MsG0080048875.01.T01:intergenic | 35.0% | |
| GGTTTGTGAATGTGTTTCAA+GGG | + | contig503end:7554-7573 | MsG0080048875.01.T01:CDS | 35.0% | |
| TACTTTGAATGCCATCTCAT+GGG | + | contig503end:7210-7229 | MsG0080048875.01.T01:CDS | 35.0% | |
| TTTCATTTCACCTTCTGGAT+GGG | + | contig503end:6754-6773 | MsG0080048875.01.T01:CDS | 35.0% | |
| ! | TTTCAAGGGTTTGAAGTGAT+GGG | + | contig503end:7568-7587 | MsG0080048875.01.T01:CDS | 35.0% |
| !! | ATTCCAAACAAGGTGGATTT+TGG | - | contig503end:7047-7066 | MsG0080048875.01.T01:intergenic | 35.0% |
| AAAAAGGCCACCATACACAA+CGG | + | contig503end:6974-6993 | MsG0080048875.01.T01:CDS | 40.0% | |
| AAAGTCCTCGATTCCAAACA+AGG | - | contig503end:7057-7076 | MsG0080048875.01.T01:intergenic | 40.0% | |
| AAATCCTACCACCACTTTGT+TGG | + | contig503end:7511-7530 | MsG0080048875.01.T01:CDS | 40.0% | |
| AATCCTACCACCACTTTGTT+GGG | + | contig503end:7512-7531 | MsG0080048875.01.T01:CDS | 40.0% | |
| ACAATGCTGAGAGTGATTCA+CGG | + | contig503end:7187-7206 | MsG0080048875.01.T01:CDS | 40.0% | |
| ACATTCGGGTCTATGATGTA+AGG | - | contig503end:6875-6894 | MsG0080048875.01.T01:intergenic | 40.0% | |
| ACTCTTGGGTGATATTCTGT+AGG | + | contig503end:7671-7690 | MsG0080048875.01.T01:CDS | 40.0% | |
| ACTTTGAATGCCATCTCATG+GGG | + | contig503end:7211-7230 | MsG0080048875.01.T01:CDS | 40.0% | |
| ATGAAGAGAGAAGGACTTGT+CGG | + | contig503end:7712-7731 | MsG0080048875.01.T01:CDS | 40.0% | |
| CAAAAGAATACCCCATGAGA+TGG | - | contig503end:7224-7243 | MsG0080048875.01.T01:intergenic | 40.0% | |
| CAACAATGCTAAAGTCTGCA+AGG | - | contig503end:7452-7471 | MsG0080048875.01.T01:intergenic | 40.0% | |
| CATTCACCTTATCTCTTCCA+TGG | + | contig503end:6934-6953 | MsG0080048875.01.T01:CDS | 40.0% | |
| CTTTCATTCTTGGAACTGTG+GGG | + | contig503end:7335-7354 | MsG0080048875.01.T01:CDS | 40.0% | |
| CTTTCATTTCACCTTCTGGA+TGG | + | contig503end:6753-6772 | MsG0080048875.01.T01:CDS | 40.0% | |
| GAATCACTCTCAGCATTGTA+AGG | - | contig503end:7186-7205 | MsG0080048875.01.T01:intergenic | 40.0% | |
| GAGATAAGGTGAATGTCAAG+TGG | - | contig503end:6929-6948 | MsG0080048875.01.T01:intergenic | 40.0% | |
| GCTTTCATTCTTGGAACTGT+GGG | + | contig503end:7334-7353 | MsG0080048875.01.T01:CDS | 40.0% | |
| GGGTTTGTGAATGTGTTTCA+AGG | + | contig503end:7553-7572 | MsG0080048875.01.T01:CDS | 40.0% | |
| GGTACTTTCATTTCACCTTC+TGG | + | contig503end:6749-6768 | MsG0080048875.01.T01:CDS | 40.0% | |
| GGTCTATGATGTAAGGAAGT+AGG | - | contig503end:6868-6887 | MsG0080048875.01.T01:intergenic | 40.0% | |
| GTACTTTGAATGCCATCTCA+TGG | + | contig503end:7209-7228 | MsG0080048875.01.T01:CDS | 40.0% | |
| GTAGAAAGTCCATGCTATAG+AGG | - | contig503end:6696-6715 | MsG0080048875.01.T01:intergenic | 40.0% | |
| TGCTTTCATTCTTGGAACTG+TGG | + | contig503end:7333-7352 | MsG0080048875.01.T01:CDS | 40.0% | |
| TGTAAGGAAGTAGGACTACT+TGG | - | contig503end:6859-6878 | MsG0080048875.01.T01:intergenic | 40.0% | |
| TTGGGACTTTCTACTCTGAT+TGG | + | contig503end:7625-7644 | MsG0080048875.01.T01:CDS | 40.0% | |
| TTGTCGGAACTTCTGACAAA+GGG | + | contig503end:7728-7747 | MsG0080048875.01.T01:CDS | 40.0% | |
| TTTGGCATAGATTTGGATGG+GGG | - | contig503end:7002-7021 | MsG0080048875.01.T01:intergenic | 40.0% | |
| ! | CGATAACTGCACGTTACTTT+AGG | + | contig503end:7254-7273 | MsG0080048875.01.T01:CDS | 40.0% |
| ! | GATTGGTGTTTCCATAGCTT+TGG | + | contig503end:7642-7661 | MsG0080048875.01.T01:CDS | 40.0% |
| ! | GGAACAAGTTCAGGAAGTAT+TGG | + | contig503end:7488-7507 | MsG0080048875.01.T01:CDS | 40.0% |
| ! | GTTTCAAGGGTTTGAAGTGA+TGG | + | contig503end:7567-7586 | MsG0080048875.01.T01:CDS | 40.0% |
| ! | TTCAAGGGTTTGAAGTGATG+GGG | + | contig503end:7569-7588 | MsG0080048875.01.T01:CDS | 40.0% |
| !! | AAAGAAGACTAGCTCTAAGG+TGG | - | contig503end:6729-6748 | MsG0080048875.01.T01:intergenic | 40.0% |
| !! | ACCAAAGAAGACTAGCTCTA+AGG | - | contig503end:6732-6751 | MsG0080048875.01.T01:intergenic | 40.0% |
| !! | ACCTTAGAGCTAGTCTTCTT+TGG | + | contig503end:6728-6747 | MsG0080048875.01.T01:CDS | 40.0% |
| !! | GAGAGAGTAGTGGAGATTTT+TGG | - | contig503end:6904-6923 | MsG0080048875.01.T01:intergenic | 40.0% |
| ACAGTAACTCAGCTTGGCAT+AGG | - | contig503end:7605-7624 | MsG0080048875.01.T01:intergenic | 45.0% | |
| ACTTCTGACAAAGGGAGTAG+TGG | + | contig503end:7736-7755 | MsG0080048875.01.T01:CDS | 45.0% | |
| AGAGTTCACCTCCAAAGCTA+TGG | - | contig503end:7656-7675 | MsG0080048875.01.T01:intergenic | 45.0% | |
| ATTTCACCTTCTGGATGGGT+TGG | + | contig503end:6758-6777 | MsG0080048875.01.T01:CDS | 45.0% | |
| CAAACGCTAGGAACAAGTTC+AGG | + | contig503end:7479-7498 | MsG0080048875.01.T01:CDS | 45.0% | |
| CCTGTCATCTCCGATGAAAT+GGG | - | contig503end:6797-6816 | MsG0080048875.01.T01:intergenic | 45.0% | |
| CTGTCATCTCCGATGAAATG+GGG | - | contig503end:6796-6815 | MsG0080048875.01.T01:intergenic | 45.0% | |
| CTTGTCGGAACTTCTGACAA+AGG | + | contig503end:7727-7746 | MsG0080048875.01.T01:CDS | 45.0% | |
| CTTTGTTGGGTACTCTTGTG+TGG | + | contig503end:7525-7544 | MsG0080048875.01.T01:CDS | 45.0% | |
| GAGGTGGTTGGATGAATAGT+AGG | - | contig503end:7097-7116 | MsG0080048875.01.T01:intergenic | 45.0% | |
| GCCAAGCTGAGTTACTGTTT+GGG | + | contig503end:7607-7626 | MsG0080048875.01.T01:CDS | 45.0% | |
| GGCCATATATGGTGGCAAAA+AGG | + | contig503end:6958-6977 | MsG0080048875.01.T01:CDS | 45.0% | |
| GGGAATCAACCCCATTTCAT+CGG | + | contig503end:6784-6803 | MsG0080048875.01.T01:CDS | 45.0% | |
| TCACCTTATCTCTTCCATGG+CGG | + | contig503end:6937-6956 | MsG0080048875.01.T01:CDS | 45.0% | |
| TCCATGCTATAGAGGCTTGT+TGG | - | contig503end:6688-6707 | MsG0080048875.01.T01:intergenic | 45.0% | |
| TCCCAAACAGTAACTCAGCT+TGG | - | contig503end:7611-7630 | MsG0080048875.01.T01:intergenic | 45.0% | |
| TCCTGTCATCTCCGATGAAA+TGG | - | contig503end:6798-6817 | MsG0080048875.01.T01:intergenic | 45.0% | |
| TGCCAAGCTGAGTTACTGTT+TGG | + | contig503end:7606-7625 | MsG0080048875.01.T01:CDS | 45.0% | |
| ! | ATCCACCTTGTTTGGAATCG+AGG | + | contig503end:7049-7068 | MsG0080048875.01.T01:CDS | 45.0% |
| ! | CCATGCTATAGAGGCTTGTT+GGG | - | contig503end:6687-6706 | MsG0080048875.01.T01:intergenic | 45.0% |
| ! | CTGAACTTGTTCCTAGCGTT+TGG | - | contig503end:7481-7500 | MsG0080048875.01.T01:intergenic | 45.0% |
| ! | GGCCTTTTTGCCACCATATA+TGG | - | contig503end:6963-6982 | MsG0080048875.01.T01:intergenic | 45.0% |
| ! | TCAAGGGTTTGAAGTGATGG+GGG | + | contig503end:7570-7589 | MsG0080048875.01.T01:CDS | 45.0% |
| ! | TGTTGTTTAGGCCAAACGCT+AGG | + | contig503end:7467-7486 | MsG0080048875.01.T01:CDS | 45.0% |
| !! | AAGAGTACCCAACAAAGTGG+TGG | - | contig503end:7522-7541 | MsG0080048875.01.T01:intergenic | 45.0% |
| !! | AGCTCTAAGGTGGAGTTATG+AGG | - | contig503end:6719-6738 | MsG0080048875.01.T01:intergenic | 45.0% |
| !! | AGCTTTGGAGGTGAACTCTT+GGG | + | contig503end:7657-7676 | MsG0080048875.01.T01:CDS | 45.0% |
| !! | ATGGGGTATTCTTTTGCCGA+TGG | + | contig503end:7228-7247 | MsG0080048875.01.T01:CDS | 45.0% |
| !! | CACAAGAGTACCCAACAAAG+TGG | - | contig503end:7525-7544 | MsG0080048875.01.T01:intergenic | 45.0% |
| !! | GCTCTAAGGTGGAGTTATGA+GGG | - | contig503end:6718-6737 | MsG0080048875.01.T01:intergenic | 45.0% |
| !! | TAGCTTTGGAGGTGAACTCT+TGG | + | contig503end:7656-7675 | MsG0080048875.01.T01:CDS | 45.0% |
| !! | TGGGGTATTCTTTTGCCGAT+GGG | + | contig503end:7229-7248 | MsG0080048875.01.T01:CDS | 45.0% |
| !! | TGGTGTTTCCATAGCTTTGG+AGG | + | contig503end:7645-7664 | MsG0080048875.01.T01:CDS | 45.0% |
| AAGGTCTGTCTGTTGACGAG+CGG | - | contig503end:7167-7186 | MsG0080048875.01.T01:intergenic | 50.0% | |
| AATGGAAGAGAGGTCAGTGG+AGG | - | contig503end:7116-7135 | MsG0080048875.01.T01:intergenic | 50.0% | |
| AGGACTACTTGGCCTGAACT+TGG | - | contig503end:6848-6867 | MsG0080048875.01.T01:intergenic | 50.0% | |
| CCCAACAAGCCTCTATAGCA+TGG | + | contig503end:6684-6703 | MsG0080048875.01.T01:CDS | 50.0% | |
| CCCATTTCATCGGAGATGAC+AGG | + | contig503end:6794-6813 | MsG0080048875.01.T01:CDS | 50.0% | |
| CTAAAGTCTGCAAGGCTCCA+AGG | - | contig503end:7444-7463 | MsG0080048875.01.T01:intergenic | 50.0% | |
| GAGTAGAGTATAGCCTCCAC+AGG | + | contig503end:7395-7414 | MsG0080048875.01.T01:CDS | 50.0% | |
| GCCTGAACTTGGGTCAGAAA+AGG | - | contig503end:6837-6856 | MsG0080048875.01.T01:intergenic | 50.0% | |
| GGACTACTTGGCCTGAACTT+GGG | - | contig503end:6847-6866 | MsG0080048875.01.T01:intergenic | 50.0% | |
| GTACCCAACAAAGTGGTGGT+AGG | - | contig503end:7518-7537 | MsG0080048875.01.T01:intergenic | 50.0% | |
| GTCCTCGATTCCAAACAAGG+TGG | - | contig503end:7054-7073 | MsG0080048875.01.T01:intergenic | 50.0% | |
| TATAGCCTCCACAGGAAGCT+TGG | + | contig503end:7403-7422 | MsG0080048875.01.T01:CDS | 50.0% | |
| TCAAGTGGTCGAGAGAGTAG+TGG | - | contig503end:6914-6933 | MsG0080048875.01.T01:intergenic | 50.0% | |
| TCTTCCATGGCGGCCATATA+TGG | + | contig503end:6947-6966 | MsG0080048875.01.T01:CDS | 50.0% | |
| TGAACCTGACAACACGTCCA+AGG | - | contig503end:7143-7162 | MsG0080048875.01.T01:intergenic | 50.0% | |
| TGGAGGCTATACTCTACTCC+TGG | - | contig503end:7394-7413 | MsG0080048875.01.T01:intergenic | 50.0% | |
| TTCAATCACTAGGTCCTGCG+TGG | + | contig503end:7281-7300 | MsG0080048875.01.T01:CDS | 50.0% | |
| ! | GCCTTTTCTGACCCAAGTTC+AGG | + | contig503end:6833-6852 | MsG0080048875.01.T01:CDS | 50.0% |
| ! | GGAATCGCTGTGTTTTGCCT+TGG | + | contig503end:7424-7443 | MsG0080048875.01.T01:CDS | 50.0% |
| !! | ACCTTCTGGATGGGTTGGAT+GGG | + | contig503end:6763-6782 | MsG0080048875.01.T01:CDS | 50.0% |
| !! | GCTATAGAGGCTTGTTGGGT+GGG | - | contig503end:6683-6702 | MsG0080048875.01.T01:intergenic | 50.0% |
| !! | TGCTATAGAGGCTTGTTGGG+TGG | - | contig503end:6684-6703 | MsG0080048875.01.T01:intergenic | 50.0% |
| ACCTGACAACACGTCCAAGG+TGG | - | contig503end:7140-7159 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CAACACGTCCAAGGTGGCAA+TGG | - | contig503end:7134-7153 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CAGCGATTCCAAGCTTCCTG+TGG | - | contig503end:7414-7433 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CAGCTTGGCATAGGATCTGC+TGG | - | contig503end:7596-7615 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CCAAGGTGGCAATGGAAGAG+AGG | - | contig503end:7126-7145 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CCTCTCTTCCATTGCCACCT+TGG | + | contig503end:7123-7142 | MsG0080048875.01.T01:CDS | 55.0% | |
| CGATTCCAAGCTTCCTGTGG+AGG | - | contig503end:7411-7430 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CGTGCAGTTATCGCTCCCAT+CGG | - | contig503end:7247-7266 | MsG0080048875.01.T01:intergenic | 55.0% | |
| CTCCTGGAGACAATTGCCCA+AGG | - | contig503end:7378-7397 | MsG0080048875.01.T01:intergenic | 55.0% | |
| GCATGAGCATAGAACCACGC+AGG | - | contig503end:7298-7317 | MsG0080048875.01.T01:intergenic | 55.0% | |
| GGCAATGGAAGAGAGGTCAG+TGG | - | contig503end:7119-7138 | MsG0080048875.01.T01:intergenic | 55.0% | |
| GGGTACTCTTGTGTGGTGCT+CGG | + | contig503end:7532-7551 | MsG0080048875.01.T01:CDS | 55.0% | |
| GGTACTCTTGTGTGGTGCTC+GGG | + | contig503end:7533-7552 | MsG0080048875.01.T01:CDS | 55.0% | |
| TCCATGGCGGCCATATATGG+TGG | + | contig503end:6950-6969 | MsG0080048875.01.T01:CDS | 55.0% | |
| TCGCCATTGGCATTCGCCTT+GGG | + | contig503end:7359-7378 | MsG0080048875.01.T01:CDS | 55.0% | |
| TGGCCGCCATGGAAGAGATA+AGG | - | contig503end:6943-6962 | MsG0080048875.01.T01:intergenic | 55.0% | |
| TTCGCCATTGGCATTCGCCT+TGG | + | contig503end:7358-7377 | MsG0080048875.01.T01:CDS | 55.0% | |
| TTGCCCAAGGCGAATGCCAA+TGG | - | contig503end:7365-7384 | MsG0080048875.01.T01:intergenic | 55.0% | |
| ! | GCGTGGTTCTATGCTCATGC+AGG | + | contig503end:7298-7317 | MsG0080048875.01.T01:CDS | 55.0% |
| !! | CACCTTCTGGATGGGTTGGA+TGG | + | contig503end:6762-6781 | MsG0080048875.01.T01:CDS | 55.0% |
| !! | CCTTCTGGATGGGTTGGATG+GGG | + | contig503end:6764-6783 | MsG0080048875.01.T01:CDS | 55.0% |
| CCCCATCCAACCCATCCAGA+AGG | - | contig503end:6767-6786 | MsG0080048875.01.T01:intergenic | 60.0% | |
| CGCCTTGGGCAATTGTCTCC+AGG | + | contig503end:7373-7392 | MsG0080048875.01.T01:CDS | 60.0% | |
| GAGAGGTCAGTGGAGGTGGT+TGG | - | contig503end:7109-7128 | MsG0080048875.01.T01:intergenic | 60.0% | |
| GCCACCATATATGGCCGCCA+TGG | - | contig503end:6954-6973 | MsG0080048875.01.T01:intergenic | 60.0% | |
| GCCACCTTGGACGTGTTGTC+AGG | + | contig503end:7136-7155 | MsG0080048875.01.T01:CDS | 60.0% | |
| GGAACTGTGGGGTTCGCCAT+TGG | + | contig503end:7346-7365 | MsG0080048875.01.T01:CDS | 60.0% | |
| GGAAGAGAGGTCAGTGGAGG+TGG | - | contig503end:7113-7132 | MsG0080048875.01.T01:intergenic | 60.0% | |
| !! | GATGGGGGCACCGTTGTGTA+TGG | - | contig503end:6987-7006 | MsG0080048875.01.T01:intergenic | 60.0% |
| !! | GGGGGCACCGTTGTGTATGG+TGG | - | contig503end:6984-7003 | MsG0080048875.01.T01:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig503end | gene | 6671 | 7771 | 6671 | ID=MsG0080048875.01;Name=MsG0080048875.01 |
| contig503end | mRNA | 6671 | 7771 | 6671 | ID=MsG0080048875.01.T01;Parent=MsG0080048875.01;Name=MsG0080048875.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|366 |
| contig503end | exon | 6671 | 7771 | 6671 | ID=MsG0080048875.01.T01:exon:924;Parent=MsG0080048875.01.T01 |
| contig503end | CDS | 6671 | 7771 | 6671 | ID=MsG0080048875.01.T01:cds;Parent=MsG0080048875.01.T01 |
| Gene Sequence |
| Protein sequence |
