Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049021.01.T01 | XP_003625561.1 | 92.875 | 393 | 22 | 2 | 1 | 390 | 1 | 390 | 0 | 754 |
| MsG0080049021.01.T01 | XP_003625561.1 | 59.322 | 59 | 23 | 1 | 187 | 244 | 362 | 420 | 7.56E-12 | 79.3 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049021.01 | MsG0180000599.01 | 0.804469 | 2.128457e-49 | 1.098289e-46 |
| MsG0080049021.01 | MsG0180004106.01 | 0.815378 | 9.532567e-52 | 6.561377e-49 |
| MsG0080049021.01 | MsG0280006914.01 | 0.806894 | 6.591586e-50 | 3.619794e-47 |
| MsG0080049021.01 | MsG0480019197.01 | 0.809871 | 1.527518e-50 | 9.071922e-48 |
| MsG0080049021.01 | MsG0580024340.01 | 0.810437 | 1.153049e-50 | 6.949980e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049021.01.T01 | MTR_7g100510 | 92.875 | 393 | 22 | 2 | 1 | 390 | 1 | 390 | 0.0 | 754 |
| MsG0080049021.01.T01 | MTR_7g100510 | 59.322 | 59 | 23 | 1 | 187 | 244 | 362 | 420 | 9.16e-16 | 79.3 |
| MsG0080049021.01.T01 | MTR_7g011820 | 45.992 | 237 | 95 | 6 | 171 | 387 | 213 | 436 | 1.24e-53 | 186 |
| MsG0080049021.01.T01 | MTR_7g011820 | 51.351 | 74 | 35 | 1 | 187 | 259 | 411 | 484 | 3.54e-15 | 77.8 |
| MsG0080049021.01.T01 | MTR_5g042920 | 46.256 | 227 | 96 | 7 | 171 | 387 | 136 | 346 | 2.82e-53 | 184 |
| MsG0080049021.01.T01 | MTR_5g042920 | 62.712 | 59 | 21 | 1 | 187 | 244 | 321 | 379 | 1.14e-16 | 82.0 |
| MsG0080049021.01.T01 | MTR_5g094430 | 41.509 | 265 | 132 | 7 | 132 | 387 | 6 | 256 | 2.26e-49 | 172 |
| MsG0080049021.01.T01 | MTR_5g094430 | 59.322 | 59 | 23 | 1 | 187 | 244 | 231 | 289 | 5.79e-15 | 76.6 |
| MsG0080049021.01.T01 | MTR_5g094430 | 41.509 | 265 | 132 | 7 | 132 | 387 | 140 | 390 | 2.80e-49 | 175 |
| MsG0080049021.01.T01 | MTR_5g094430 | 59.322 | 59 | 23 | 1 | 187 | 244 | 365 | 423 | 4.40e-15 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049021.01.T01 | AT2G37260 | 39.904 | 416 | 185 | 15 | 2 | 390 | 1 | 378 | 2.20e-80 | 254 |
| MsG0080049021.01.T01 | AT2G37260 | 58.929 | 56 | 22 | 1 | 187 | 241 | 350 | 405 | 4.31e-14 | 73.9 |
| MsG0080049021.01.T01 | AT2G37260 | 40.541 | 333 | 135 | 11 | 81 | 390 | 4 | 296 | 5.08e-62 | 204 |
| MsG0080049021.01.T01 | AT2G37260 | 58.929 | 56 | 22 | 1 | 187 | 241 | 268 | 323 | 4.60e-14 | 73.6 |
| MsG0080049021.01.T01 | AT2G37260 | 40.240 | 333 | 138 | 11 | 81 | 390 | 4 | 298 | 9.32e-61 | 201 |
| MsG0080049021.01.T01 | AT2G37260 | 58.929 | 56 | 22 | 1 | 187 | 241 | 270 | 325 | 4.54e-14 | 73.6 |
| MsG0080049021.01.T01 | AT2G03340 | 49.000 | 200 | 93 | 4 | 180 | 378 | 244 | 435 | 6.75e-55 | 190 |
| MsG0080049021.01.T01 | AT2G03340 | 61.017 | 59 | 22 | 1 | 187 | 244 | 416 | 474 | 9.74e-17 | 82.4 |
| MsG0080049021.01.T01 | AT1G13960 | 44.545 | 220 | 103 | 4 | 180 | 387 | 70 | 282 | 2.25e-52 | 179 |
| MsG0080049021.01.T01 | AT1G13960 | 61.017 | 59 | 22 | 1 | 187 | 244 | 257 | 315 | 2.09e-16 | 80.5 |
Find 92 sgRNAs with CRISPR-Local
Find 177 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTATAAACCTGGGATA+TGG | 0.347198 | contig614end:+140 | None:intergenic |
| AAAATTCTTGTGGTCTAAGC+GGG | 0.435521 | contig614end:-882 | MsG0080049021.01.T01:CDS |
| AAACTACAATATCCGCCATT+AGG | 0.452468 | contig614end:-2540 | MsG0080049021.01.T01:CDS |
| AAAGTTGAAAGATCATTAGA+TGG | 0.489403 | contig614end:-1111 | MsG0080049021.01.T01:CDS |
| AAATGAAGTGGCAGTATCAG+AGG | 0.580061 | contig614end:-270 | MsG0080049021.01.T01:CDS |
| AAATTCTTGTGGTCTAAGCG+GGG | 0.685313 | contig614end:-881 | MsG0080049021.01.T01:CDS |
| AAGAAGAAAAGTATAAACCT+GGG | 0.626787 | contig614end:+134 | None:intergenic |
| AAGCAAAGGATTTGAGGCAG+AGG | 0.578305 | contig614end:-845 | MsG0080049021.01.T01:CDS |
| AATCAGAATGAAAGGAATGA+AGG | 0.486904 | contig614end:-955 | MsG0080049021.01.T01:CDS |
| ACAAACAACTGTCTCTCTCT+TGG | 0.497528 | contig614end:-2193 | MsG0080049021.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGAACTTGTAAAGTGAG+AGG | - | contig614end:1145-1164 | MsG0080049021.01.T01:CDS | 30.0% | |
| AATCAGAATGAAAGGAATGA+AGG | - | contig614end:1752-1771 | MsG0080049021.01.T01:intron | 30.0% | |
| AGTCACTAACACAAATATAG+CGG | + | contig614end:1088-1107 | None:intergenic | 30.0% | |
| ATGAACAACATTTGTAAGTG+AGG | - | contig614end:976-995 | MsG0080049021.01.T01:CDS | 30.0% | |
| ATGACCATAGAAGTAAAAGA+AGG | - | contig614end:1888-1907 | MsG0080049021.01.T01:intron | 30.0% | |
| CTAGCTTATAGAGTTAAGTT+AGG | - | contig614end:819-838 | MsG0080049021.01.T01:intron | 30.0% | |
| GAATGGTTCAAGATAAAGTA+TGG | - | contig614end:1717-1736 | MsG0080049021.01.T01:intron | 30.0% | |
| GATTTGTAAGAAGAACAAGT+AGG | + | contig614end:91-110 | None:intergenic | 30.0% | |
| GGATATAACTGGAGAAAATA+TGG | - | contig614end:1497-1516 | MsG0080049021.01.T01:intron | 30.0% | |
| TCAAGATTACTTATACAGCA+AGG | - | contig614end:2632-2651 | MsG0080049021.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig614end | gene | 31 | 2698 | 31 | ID=MsG0080049021.01;Name=MsG0080049021.01 |
| contig614end | mRNA | 31 | 2698 | 31 | ID=MsG0080049021.01.T01;Parent=MsG0080049021.01;Name=MsG0080049021.01.T01;_AED=0.36;_eAED=0.41;_QI=0|0|0|1|1|1|4|0|417 |
| contig614end | exon | 2492 | 2698 | 2492 | ID=MsG0080049021.01.T01:exon:17514;Parent=MsG0080049021.01.T01 |
| contig614end | exon | 2185 | 2307 | 2185 | ID=MsG0080049021.01.T01:exon:17513;Parent=MsG0080049021.01.T01 |
| contig614end | exon | 820 | 1463 | 820 | ID=MsG0080049021.01.T01:exon:17512;Parent=MsG0080049021.01.T01 |
| contig614end | exon | 31 | 310 | 31 | ID=MsG0080049021.01.T01:exon:17511;Parent=MsG0080049021.01.T01 |
| contig614end | CDS | 2492 | 2698 | 2492 | ID=MsG0080049021.01.T01:cds;Parent=MsG0080049021.01.T01 |
| contig614end | CDS | 2185 | 2307 | 2185 | ID=MsG0080049021.01.T01:cds;Parent=MsG0080049021.01.T01 |
| contig614end | CDS | 820 | 1463 | 820 | ID=MsG0080049021.01.T01:cds;Parent=MsG0080049021.01.T01 |
| contig614end | CDS | 31 | 310 | 31 | ID=MsG0080049021.01.T01:cds;Parent=MsG0080049021.01.T01 |
| Gene Sequence |
>MsG0080049021.01.T01 ATGATGGATATTGAAGAAGCAGAAAGAGTAGTTGTTGCCAAGCCAGTTGCTTCAAGGCCTACTTGTTCTTCTTACAAATCTTTCTCAGAGCTTCTAGCAGGTGCAATCAACTCCACTCCTCCAATTGCATCTTCTCAAACTACAATATCCGCCATTAGGCCAAAGACAGTGAGGTTCAAGCCAGCAATGAATCTCCCCCCTCCTCAGGCTAATATTGCACTTAGTAGTTCTTCTGACATGGATCCGAAACCTGACACCAATCAATCTCTTATTTACAAACCAATGGCAAAATTTGTGTCACAAACAACTGTCTCTCTCTTGGCAAACATGGGAATTTGCAGTACTAATCAGCAACAACCACAAAAATCAATGGAGACCAATCCTCAGCATCTTAACCATGAAAACTTTAGAGCTAACACGAGCTCGAAATTACATCAGAACATTACCCTTCCTACAGAAACGTATCAGGCCACAGAATCTTGTACGATGGCACCACAGAACATGGAGGAGGACCAAAAAGCATTACCATCCACAAATAATGCTGATAGACCTTCGTATGATGGATATAACTGGAGAAAATATGGACAGAAGCAAGTAAAAGGAAGCGAGTATCCTCGAAGTTACTACAAGTGCACATATCCTAATTGTCCAGTTAAAAAGAAAGTTGAAAGATCATTAGATGGTGAGATTGCAGAAATTGTTTATAAAGGTGAGCACAACCATGGAAAGCCACAACATCAAAAGCGAAACTCGGGAGCAACATCAGGGATGATTTCTGATGGAATGGTTCAAGATAAAGTATGGAGTAATAATAGTAATCAGAATGAAAGGAATGAAGGAAGAATAGAGAATAAAGTCAAAGCTTCACAGCCTAATGATTTTGCTCTAGAAAATTCTTGTGGTCTAAGCGGGGAATGTGAGGAAGGAAGCAAAGGATTTGAGGCAGAGGAAGATGACCATAGAAGTAAAAGAAGGAAAAATGAGAATCAATCAAATGAAGTGGCAGTATCAGAGGAAGGTCTAGTAGAGCCAGGCATTGTAATGCAAAGTTCTGTGGATTCTGAAGTCCTTGGAGATGGCTTTCGCTGGAGGAAATATGGGCAGAAGGTGGTGAAGGGAAATCCATATCCCAGGTTTATACTTTTCTTCTTCAAAGTAACTTTTATAAATGAGCTGTTTCAAGATTTTCAAGATTACTTATACAGCAAGGTTCTAAATTCTGCTATGCATCGGCCCAAAATATCGAGTACATAG |
| Protein sequence |
>MsG0080049021.01.T01 MMDIEEAERVVVAKPVASRPTCSSYKSFSELLAGAINSTPPIASSQTTISAIRPKTVRFKPAMNLPPPQANIALSSSSDMDPKPDTNQSLIYKPMAKFVSQTTVSLLANMGICSTNQQQPQKSMETNPQHLNHENFRANTSSKLHQNITLPTETYQATESCTMAPQNMEEDQKALPSTNNADRPSYDGYNWRKYGQKQVKGSEYPRSYYKCTYPNCPVKKKVERSLDGEIAEIVYKGEHNHGKPQHQKRNSGATSGMISDGMVQDKVWSNNSNQNERNEGRIENKVKASQPNDFALENSCGLSGECEEGSKGFEAEEDDHRSKRRKNENQSNEVAVSEEGLVEPGIVMQSSVDSEVLGDGFRWRKYGQKVVKGNPYPRFILFFFKVTFINELFQDFQDYLYSKVLNSAMHRPKISST* |
