Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049139.01.T01 | XP_003588863.1 | 96.262 | 107 | 3 | 1 | 1 | 106 | 1 | 107 | 1.11E-67 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049139.01.T01 | G7I882 | 96.262 | 107 | 3 | 1 | 1 | 106 | 1 | 107 | 5.32e-68 | 208 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049139.01 | MsG0280008417.01 | 0.822978 | 1.773895e-53 | 1.507715e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049139.01.T01 | MTR_1g014110 | 96.262 | 107 | 3 | 1 | 1 | 106 | 1 | 107 | 1.35e-71 | 208 |
| MsG0080049139.01.T01 | MTR_4g130810 | 59.016 | 61 | 25 | 0 | 45 | 105 | 20 | 80 | 1.15e-21 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049139.01.T01 | AT4G31830 | 66.337 | 101 | 29 | 1 | 6 | 106 | 5 | 100 | 3.59e-44 | 139 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACAAGTAGTTATTTCTT+AGG | 0.265835 | contig92end:+24405 | None:intergenic |
| TACTATGACTATTGGTGGTT+TGG | 0.317364 | contig92end:-24541 | MsG0080049139.01.T01:CDS |
| AATTTCACTACTATGACTAT+TGG | 0.363054 | contig92end:-24549 | MsG0080049139.01.T01:CDS |
| AGATGCTTCAGGCTTCTTCT+TGG | 0.383529 | contig92end:+24482 | None:intergenic |
| GCTGAGCCATGATTACTTCC+TGG | 0.397108 | contig92end:+24615 | None:intergenic |
| ACATCAACAGCAGATGCTTC+AGG | 0.412315 | contig92end:+24471 | None:intergenic |
| TTGGTTATTACAGCTGCTAT+TGG | 0.423221 | contig92end:-24522 | MsG0080049139.01.T01:CDS |
| TTCTTAGGATGAGTGTCCTC+AGG | 0.444254 | contig92end:+24420 | None:intergenic |
| TTCACTACTATGACTATTGG+TGG | 0.451309 | contig92end:-24546 | MsG0080049139.01.T01:CDS |
| TGAGTACTACAAACGAAGAT+TGG | 0.458170 | contig92end:-24713 | MsG0080049139.01.T01:CDS |
| GTCATAGTAGTGAAATTGAA+TGG | 0.471610 | contig92end:+24555 | None:intergenic |
| CTTCTTCTTGGTATACAAAA+CGG | 0.472871 | contig92end:+24494 | None:intergenic |
| GATGTTGCTAAGGTTACTGC+TGG | 0.510228 | contig92end:-24453 | MsG0080049139.01.T01:CDS |
| AGTAATCATGGCTCAGCAGC+TGG | 0.525982 | contig92end:-24609 | MsG0080049139.01.T01:CDS |
| GTAAAAGCAGCAGGAGTGGA+AGG | 0.527140 | contig92end:-24651 | MsG0080049139.01.T01:CDS |
| TCAGAAGAAGTAAAAGCAGC+AGG | 0.534308 | contig92end:-24660 | MsG0080049139.01.T01:CDS |
| AGACCACCAGGAAGTAATCA+TGG | 0.535373 | contig92end:-24621 | MsG0080049139.01.T01:CDS |
| ATCTGCTGTTGATGTTGCTA+AGG | 0.538816 | contig92end:-24463 | MsG0080049139.01.T01:CDS |
| GTGCAAATGTTCTTCATCAA+AGG | 0.539981 | contig92end:-24587 | MsG0080049139.01.T01:CDS |
| AGAAGTAAAAGCAGCAGGAG+TGG | 0.552330 | contig92end:-24655 | MsG0080049139.01.T01:CDS |
| GAAGGTTCAAAGAGACCACC+AGG | 0.575765 | contig92end:-24633 | MsG0080049139.01.T01:CDS |
| TGCTGGAGTTGCAAATCCTG+AGG | 0.647645 | contig92end:-24436 | MsG0080049139.01.T01:CDS |
| GAGCCATGATTACTTCCTGG+TGG | 0.667121 | contig92end:+24618 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTCACTACTATGACTAT+TGG | - | contig92end:24581-24600 | MsG0080049139.01.T01:CDS | 25.0% |
| CTTCTTCTTGGTATACAAAA+CGG | + | contig92end:24639-24658 | None:intergenic | 30.0% | |
| GTCATAGTAGTGAAATTGAA+TGG | + | contig92end:24578-24597 | None:intergenic | 30.0% | |
| GTGCAAATGTTCTTCATCAA+AGG | - | contig92end:24543-24562 | MsG0080049139.01.T01:CDS | 35.0% | |
| TGAGTACTACAAACGAAGAT+TGG | - | contig92end:24417-24436 | MsG0080049139.01.T01:CDS | 35.0% | |
| TTCACTACTATGACTATTGG+TGG | - | contig92end:24584-24603 | MsG0080049139.01.T01:CDS | 35.0% | |
| TTGGTTATTACAGCTGCTAT+TGG | - | contig92end:24608-24627 | MsG0080049139.01.T01:CDS | 35.0% | |
| ! | TACTATGACTATTGGTGGTT+TGG | - | contig92end:24589-24608 | MsG0080049139.01.T01:CDS | 35.0% |
| ATCTGCTGTTGATGTTGCTA+AGG | - | contig92end:24667-24686 | MsG0080049139.01.T01:CDS | 40.0% | |
| TCAGAAGAAGTAAAAGCAGC+AGG | - | contig92end:24470-24489 | MsG0080049139.01.T01:CDS | 40.0% | |
| ACATCAACAGCAGATGCTTC+AGG | + | contig92end:24662-24681 | None:intergenic | 45.0% | |
| AGAAGTAAAAGCAGCAGGAG+TGG | - | contig92end:24475-24494 | MsG0080049139.01.T01:CDS | 45.0% | |
| AGACCACCAGGAAGTAATCA+TGG | - | contig92end:24509-24528 | MsG0080049139.01.T01:CDS | 45.0% | |
| AGATGCTTCAGGCTTCTTCT+TGG | + | contig92end:24651-24670 | None:intergenic | 45.0% | |
| GATGTTGCTAAGGTTACTGC+TGG | - | contig92end:24677-24696 | MsG0080049139.01.T01:CDS | 45.0% | |
| TTCTTAGGATGAGTGTCCTC+AGG | + | contig92end:24713-24732 | None:intergenic | 45.0% | |
| AGTAATCATGGCTCAGCAGC+TGG | - | contig92end:24521-24540 | MsG0080049139.01.T01:CDS | 50.0% | |
| GAAGGTTCAAAGAGACCACC+AGG | - | contig92end:24497-24516 | MsG0080049139.01.T01:CDS | 50.0% | |
| GAGCCATGATTACTTCCTGG+TGG | + | contig92end:24515-24534 | None:intergenic | 50.0% | |
| GCTGAGCCATGATTACTTCC+TGG | + | contig92end:24518-24537 | None:intergenic | 50.0% | |
| GTAAAAGCAGCAGGAGTGGA+AGG | - | contig92end:24479-24498 | MsG0080049139.01.T01:CDS | 50.0% | |
| TGCTGGAGTTGCAAATCCTG+AGG | - | contig92end:24694-24713 | MsG0080049139.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig92end | gene | 24416 | 24736 | 24416 | ID=MsG0080049139.01;Name=MsG0080049139.01 |
| contig92end | mRNA | 24416 | 24736 | 24416 | ID=MsG0080049139.01.T01;Parent=MsG0080049139.01;Name=MsG0080049139.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|106 |
| contig92end | exon | 24416 | 24736 | 24416 | ID=MsG0080049139.01.T01:exon:1390;Parent=MsG0080049139.01.T01 |
| contig92end | CDS | 24416 | 24736 | 24416 | ID=MsG0080049139.01.T01:cds;Parent=MsG0080049139.01.T01 |
| Gene Sequence |
| Protein sequence |
