Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000180.01.T01 | XP_013466055.1 | 96.19 | 105 | 4 | 0 | 1 | 105 | 250 | 354 | 2.19E-70 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000180.01.T01 | O64823 | 63.415 | 82 | 28 | 1 | 1 | 80 | 246 | 327 | 9.52E-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000180.01.T01 | A0A072VDJ2 | 96.190 | 105 | 4 | 0 | 1 | 105 | 250 | 354 | 1.05e-70 | 224 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000180.01 | MsG0380017293.01 | 0.800754 | 1.242458e-48 | 5.831673e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000180.01.T01 | MTR_1g021955 | 96.190 | 105 | 4 | 0 | 1 | 105 | 250 | 354 | 2.65e-74 | 224 |
| MsG0180000180.01.T01 | MTR_5g019350 | 57.143 | 77 | 33 | 0 | 1 | 77 | 248 | 324 | 3.98e-32 | 115 |
| MsG0180000180.01.T01 | MTR_8g078170 | 59.740 | 77 | 31 | 0 | 1 | 77 | 253 | 329 | 4.23e-30 | 110 |
| MsG0180000180.01.T01 | MTR_2091s0010 | 53.409 | 88 | 32 | 1 | 1 | 79 | 167 | 254 | 1.48e-28 | 104 |
| MsG0180000180.01.T01 | MTR_5g076060 | 50.685 | 73 | 36 | 0 | 2 | 74 | 234 | 306 | 3.07e-22 | 89.0 |
| MsG0180000180.01.T01 | MTR_3g063050 | 48.718 | 78 | 40 | 0 | 2 | 79 | 241 | 318 | 6.72e-21 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000180.01.T01 | AT2G23790 | 63.415 | 82 | 28 | 1 | 1 | 80 | 246 | 327 | 9.70e-34 | 119 |
| MsG0180000180.01.T01 | AT5G42610 | 61.905 | 84 | 24 | 1 | 1 | 76 | 209 | 292 | 8.66e-32 | 113 |
| MsG0180000180.01.T01 | AT4G36820 | 57.895 | 76 | 32 | 0 | 1 | 76 | 248 | 323 | 1.34e-30 | 111 |
| MsG0180000180.01.T01 | AT5G66650 | 57.895 | 76 | 32 | 0 | 2 | 77 | 239 | 314 | 3.08e-28 | 104 |
| MsG0180000180.01.T01 | AT1G57610 | 50.000 | 70 | 35 | 0 | 1 | 70 | 184 | 253 | 1.05e-21 | 87.0 |
| MsG0180000180.01.T01 | AT1G57610 | 50.000 | 70 | 35 | 0 | 1 | 70 | 184 | 253 | 1.05e-21 | 87.0 |
| MsG0180000180.01.T01 | AT1G09575 | 49.275 | 69 | 35 | 0 | 2 | 70 | 189 | 257 | 1.05e-20 | 84.3 |
| MsG0180000180.01.T01 | AT1G09575 | 49.275 | 69 | 35 | 0 | 2 | 70 | 189 | 257 | 1.05e-20 | 84.3 |
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATGAGACTCACATTT+TGG | 0.186995 | 1:+2483004 | None:intergenic |
| GGAGCTAAGTTGGGATGTTA+TGG | 0.328705 | 1:+2483026 | MsG0180000180.01.T01:CDS |
| CTATTTCCATCGTCGTTTCA+TGG | 0.338829 | 1:+2483137 | MsG0180000180.01.T01:CDS |
| TCAACTGAGCCAACATTTCA+AGG | 0.366528 | 1:+2483114 | MsG0180000180.01.T01:CDS |
| CATACAACTTTGATGCAAAT+AGG | 0.382038 | 1:+2483184 | MsG0180000180.01.T01:CDS |
| TTGCATACTTATTCTTCATA+AGG | 0.383459 | 1:+2483088 | MsG0180000180.01.T01:CDS |
| CCAACACCAACATGATTACT+TGG | 0.408218 | 1:-2483234 | None:intergenic |
| GAAGTAACAAAGAAACATAT+TGG | 0.436866 | 1:-2483051 | None:intergenic |
| TGGAAATAGCCTTGAAATGT+TGG | 0.476468 | 1:-2483123 | None:intergenic |
| GAAGTGTCTCTTCAGCATGC+TGG | 0.498390 | 1:-2483293 | None:intergenic |
| AGTATGCAAAAGCAAAGTGC+AGG | 0.503609 | 1:-2483074 | None:intergenic |
| GTCTCTTCAGCATGCTGGAT+AGG | 0.533472 | 1:-2483288 | None:intergenic |
| TCTTCAGCATGCTGGATAGG+TGG | 0.569356 | 1:-2483285 | None:intergenic |
| CCAAGTAATCATGTTGGTGT+TGG | 0.577920 | 1:+2483234 | MsG0180000180.01.T01:CDS |
| TGTTATCCAAGTAATCATGT+TGG | 0.620660 | 1:+2483228 | MsG0180000180.01.T01:CDS |
| GTATGCAAAAGCAAAGTGCA+GGG | 0.637114 | 1:-2483073 | None:intergenic |
| GTTTAGCCATGAAACGACGA+TGG | 0.674204 | 1:-2483143 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGTAACAAAGAAACATAT+TGG | - | Chr1:2483054-2483073 | None:intergenic | 25.0% |
| ! | TTGCATACTTATTCTTCATA+AGG | + | Chr1:2483088-2483107 | MsG0180000180.01.T01:CDS | 25.0% |
| CATACAACTTTGATGCAAAT+AGG | + | Chr1:2483184-2483203 | MsG0180000180.01.T01:CDS | 30.0% | |
| TGTTATCCAAGTAATCATGT+TGG | + | Chr1:2483228-2483247 | MsG0180000180.01.T01:CDS | 30.0% | |
| ! | TGGAAATAGCCTTGAAATGT+TGG | - | Chr1:2483126-2483145 | None:intergenic | 35.0% |
| AGTATGCAAAAGCAAAGTGC+AGG | - | Chr1:2483077-2483096 | None:intergenic | 40.0% | |
| CCAACACCAACATGATTACT+TGG | - | Chr1:2483237-2483256 | None:intergenic | 40.0% | |
| CCAAGTAATCATGTTGGTGT+TGG | + | Chr1:2483234-2483253 | MsG0180000180.01.T01:CDS | 40.0% | |
| CTATTTCCATCGTCGTTTCA+TGG | + | Chr1:2483137-2483156 | MsG0180000180.01.T01:CDS | 40.0% | |
| GTATGCAAAAGCAAAGTGCA+GGG | - | Chr1:2483076-2483095 | None:intergenic | 40.0% | |
| TCAACTGAGCCAACATTTCA+AGG | + | Chr1:2483114-2483133 | MsG0180000180.01.T01:CDS | 40.0% | |
| !! | CACATTTTGGGAGCTAAGTT+GGG | + | Chr1:2483017-2483036 | MsG0180000180.01.T01:CDS | 40.0% |
| !! | TCACATTTTGGGAGCTAAGT+TGG | + | Chr1:2483016-2483035 | MsG0180000180.01.T01:CDS | 40.0% |
| GTTTAGCCATGAAACGACGA+TGG | - | Chr1:2483146-2483165 | None:intergenic | 45.0% | |
| ! | GGAGCTAAGTTGGGATGTTA+TGG | + | Chr1:2483026-2483045 | MsG0180000180.01.T01:CDS | 45.0% |
| GTCTCTTCAGCATGCTGGAT+AGG | - | Chr1:2483291-2483310 | None:intergenic | 50.0% | |
| TCTTCAGCATGCTGGATAGG+TGG | - | Chr1:2483288-2483307 | None:intergenic | 50.0% | |
| ! | GAAGTGTCTCTTCAGCATGC+TGG | - | Chr1:2483296-2483315 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 2483009 | 2483326 | 2483009 | ID=MsG0180000180.01;Name=MsG0180000180.01 |
| Chr1 | mRNA | 2483009 | 2483326 | 2483009 | ID=MsG0180000180.01.T01;Parent=MsG0180000180.01;Name=MsG0180000180.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|105 |
| Chr1 | exon | 2483009 | 2483326 | 2483009 | ID=MsG0180000180.01.T01:exon:29297;Parent=MsG0180000180.01.T01 |
| Chr1 | CDS | 2483009 | 2483326 | 2483009 | ID=MsG0180000180.01.T01:cds;Parent=MsG0180000180.01.T01 |
| Gene Sequence |
| Protein sequence |
