Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000296.01.T01 | XP_003589126.1 | 100 | 136 | 0 | 0 | 6 | 141 | 190 | 325 | 1.16E-90 | 276 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000296.01.T01 | Q8GXG1 | 83.824 | 136 | 22 | 0 | 6 | 141 | 190 | 325 | 1.35E-76 | 233 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000296.01.T01 | Q2HTR7 | 100.000 | 136 | 0 | 0 | 6 | 141 | 190 | 325 | 5.52e-91 | 276 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000295.01 | MsG0180000296.01 | 0.905400 | 4.469833e-80 | 7.216651e-76 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000296.01.T01 | MTR_1g018760 | 100.000 | 136 | 0 | 0 | 6 | 141 | 190 | 325 | 1.40e-94 | 276 |
| MsG0180000296.01.T01 | MTR_3g102370 | 88.235 | 136 | 16 | 0 | 6 | 141 | 187 | 322 | 5.88e-84 | 249 |
| MsG0180000296.01.T01 | MTR_1g094030 | 87.407 | 135 | 17 | 0 | 7 | 141 | 186 | 320 | 7.24e-82 | 243 |
| MsG0180000296.01.T01 | MTR_4g109900 | 64.615 | 130 | 46 | 0 | 11 | 140 | 189 | 318 | 1.44e-54 | 174 |
| MsG0180000296.01.T01 | MTR_4g109920 | 64.615 | 130 | 46 | 0 | 11 | 140 | 190 | 319 | 1.79e-54 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000296.01.T01 | AT3G16150 | 83.824 | 136 | 22 | 0 | 6 | 141 | 190 | 325 | 1.38e-77 | 233 |
| MsG0180000296.01.T01 | AT5G08100 | 61.069 | 131 | 51 | 0 | 11 | 141 | 103 | 233 | 3.05e-53 | 168 |
| MsG0180000296.01.T01 | AT5G08100 | 61.538 | 130 | 50 | 0 | 11 | 140 | 183 | 312 | 2.89e-52 | 168 |
Find 51 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATGGAGGTTGGAATT+TGG | 0.195033 | 1:+4097979 | MsG0180000296.01.T01:CDS |
| ACTACGCACCCGACTGTTTC+AGG | 0.214500 | 1:-4097606 | None:intergenic |
| GTTCATGGAGGTTGGAATTT+GGG | 0.262833 | 1:+4097980 | MsG0180000296.01.T01:CDS |
| TTAACTGCAATGGCATGTTT+AGG | 0.291387 | 1:+4097937 | MsG0180000296.01.T01:CDS |
| AGTAAAGGAGAAGTTGCTTA+TGG | 0.336064 | 1:+4097912 | MsG0180000296.01.T01:CDS |
| TAACTGCAATGGCATGTTTA+GGG | 0.343986 | 1:+4097938 | MsG0180000296.01.T01:CDS |
| TGTGCTGCTGCCACGTCAAC+AGG | 0.356128 | 1:+4097648 | MsG0180000296.01.T01:CDS |
| AGGGGATGTGCTTCTGAGGA+TGG | 0.401581 | 1:+4097957 | MsG0180000296.01.T01:CDS |
| TGCTTCTGAGGATGGGTTCA+TGG | 0.405891 | 1:+4097965 | MsG0180000296.01.T01:CDS |
| CTCATTGCTGTTTCTAGTAA+AGG | 0.414337 | 1:+4097897 | MsG0180000296.01.T01:CDS |
| ATTGGTGACTCACCTTTGAT+AGG | 0.420480 | 1:+4097699 | MsG0180000296.01.T01:CDS |
| CACTTTGGCGCGAGAGGTGT+CGG | 0.430026 | 1:+4097791 | MsG0180000296.01.T01:CDS |
| ATTAAGAATCGTTTGGATGA+AGG | 0.439748 | 1:+4097864 | MsG0180000296.01.T01:CDS |
| GGGGATGTGCTTCTGAGGAT+GGG | 0.460240 | 1:+4097958 | MsG0180000296.01.T01:CDS |
| CAGCAGCACACCTTCCTTCA+CGG | 0.468263 | 1:-4097635 | None:intergenic |
| GACTCACCTTTGATAGGAGC+CGG | 0.472240 | 1:+4097705 | MsG0180000296.01.T01:CDS |
| ACCTACGCGTGTAACCTTGC+TGG | 0.480120 | 1:+4097729 | MsG0180000296.01.T01:CDS |
| GAGGATGGGTTCATGGAGGT+TGG | 0.480884 | 1:+4097972 | MsG0180000296.01.T01:CDS |
| GCTTATGGATTTAACTGCAA+TGG | 0.484263 | 1:+4097927 | MsG0180000296.01.T01:CDS |
| AAGGTTACACGCGTAGGTCC+CGG | 0.486913 | 1:-4097724 | None:intergenic |
| AACAGTCGGGTGCGTAGTTG+TGG | 0.493878 | 1:+4097611 | MsG0180000296.01.T01:CDS |
| TATGATAGCCTCACCTTCAC+CGG | 0.497356 | 1:-4097763 | None:intergenic |
| CGTTTGGATGAAGGATATGC+TGG | 0.504298 | 1:+4097873 | MsG0180000296.01.T01:CDS |
| GTGATGGAATACAAAGGTCT+TGG | 0.504451 | 1:+4097816 | MsG0180000296.01.T01:CDS |
| AACAAAATGATTGGTCGGAT+TGG | 0.504757 | 1:+4097681 | MsG0180000296.01.T01:CDS |
| GGGCTAATGAACAAAATGAT+TGG | 0.512413 | 1:+4097672 | MsG0180000296.01.T01:CDS |
| GCGAGAGGTGTCGGCTGTGA+TGG | 0.520900 | 1:+4097800 | MsG0180000296.01.T01:CDS |
| GGCGTCTCATGCACCGGTGA+AGG | 0.524434 | 1:+4097750 | MsG0180000296.01.T01:CDS |
| GGCTATCATACGTGGCACTT+TGG | 0.535143 | 1:+4097776 | MsG0180000296.01.T01:CDS |
| GCTGCTGCCACGTCAACAGG+CGG | 0.537860 | 1:+4097651 | MsG0180000296.01.T01:CDS |
| CTTGCTGGCGTCTCATGCAC+CGG | 0.541455 | 1:+4097744 | MsG0180000296.01.T01:CDS |
| CATTAGCCCGCCTGTTGACG+TGG | 0.549451 | 1:-4097658 | None:intergenic |
| CTGCTGCCACGTCAACAGGC+GGG | 0.561806 | 1:+4097652 | MsG0180000296.01.T01:CDS |
| TAATGAACAAAATGATTGGT+CGG | 0.563124 | 1:+4097676 | MsG0180000296.01.T01:CDS |
| GTTTAGGGGATGTGCTTCTG+AGG | 0.570942 | 1:+4097953 | MsG0180000296.01.T01:CDS |
| TCTACGCACCTGAAACAGTC+GGG | 0.573467 | 1:+4097598 | MsG0180000296.01.T01:CDS |
| ACGTGGCACTTTGGCGCGAG+AGG | 0.574745 | 1:+4097785 | MsG0180000296.01.T01:CDS |
| TAGGTCCCGGCTCCTATCAA+AGG | 0.574816 | 1:-4097711 | None:intergenic |
| AACTGCAATGGCATGTTTAG+GGG | 0.582673 | 1:+4097939 | MsG0180000296.01.T01:CDS |
| TTTCAGGTGCGTAGACACAT+TGG | 0.589740 | 1:-4097590 | None:intergenic |
| CTCATGCACCGGTGAAGGTG+AGG | 0.595337 | 1:+4097755 | MsG0180000296.01.T01:CDS |
| TCTTGGTCTTCAAGAAGCAG+TGG | 0.595757 | 1:+4097833 | MsG0180000296.01.T01:CDS |
| ACTCACCTTTGATAGGAGCC+GGG | 0.601444 | 1:+4097706 | MsG0180000296.01.T01:CDS |
| TGCGTAGTTGTGGACCGTGA+AGG | 0.611085 | 1:+4097621 | MsG0180000296.01.T01:CDS |
| GTCTACGCACCTGAAACAGT+CGG | 0.620759 | 1:+4097597 | MsG0180000296.01.T01:CDS |
| CGGTGCATGAGACGCCAGCA+AGG | 0.648195 | 1:-4097743 | None:intergenic |
| TAGTTGTGGACCGTGAAGGA+AGG | 0.661356 | 1:+4097625 | MsG0180000296.01.T01:CDS |
| GCCAGCAAGGTTACACGCGT+AGG | 0.682958 | 1:-4097730 | None:intergenic |
| GAAGGTGAGGCTATCATACG+TGG | 0.685255 | 1:+4097768 | MsG0180000296.01.T01:CDS |
| TTCTGAGGATGGGTTCATGG+AGG | 0.702330 | 1:+4097968 | MsG0180000296.01.T01:CDS |
| TCGGCTGTGATGGAATACAA+AGG | 0.710713 | 1:+4097810 | MsG0180000296.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATGAACAAAATGATTGGT+CGG | + | Chr1:4097676-4097695 | MsG0180000296.01.T01:CDS | 25.0% |
| !! | TTTTGTCATTAAGAATCGTT+TGG | + | Chr1:4097857-4097876 | MsG0180000296.01.T01:CDS | 25.0% |
| ATTAAGAATCGTTTGGATGA+AGG | + | Chr1:4097864-4097883 | MsG0180000296.01.T01:CDS | 30.0% | |
| AGTAAAGGAGAAGTTGCTTA+TGG | + | Chr1:4097912-4097931 | MsG0180000296.01.T01:CDS | 35.0% | |
| GCTTATGGATTTAACTGCAA+TGG | + | Chr1:4097927-4097946 | MsG0180000296.01.T01:CDS | 35.0% | |
| GGGCTAATGAACAAAATGAT+TGG | + | Chr1:4097672-4097691 | MsG0180000296.01.T01:CDS | 35.0% | |
| TAACTGCAATGGCATGTTTA+GGG | + | Chr1:4097938-4097957 | MsG0180000296.01.T01:CDS | 35.0% | |
| TTAACTGCAATGGCATGTTT+AGG | + | Chr1:4097937-4097956 | MsG0180000296.01.T01:CDS | 35.0% | |
| ! | AACAAAATGATTGGTCGGAT+TGG | + | Chr1:4097681-4097700 | MsG0180000296.01.T01:CDS | 35.0% |
| !! | CTCATTGCTGTTTCTAGTAA+AGG | + | Chr1:4097897-4097916 | MsG0180000296.01.T01:CDS | 35.0% |
| AACTGCAATGGCATGTTTAG+GGG | + | Chr1:4097939-4097958 | MsG0180000296.01.T01:CDS | 40.0% | |
| GTTCATGGAGGTTGGAATTT+GGG | + | Chr1:4097980-4097999 | MsG0180000296.01.T01:CDS | 40.0% | |
| ! | ATTGGTGACTCACCTTTGAT+AGG | + | Chr1:4097699-4097718 | MsG0180000296.01.T01:CDS | 40.0% |
| ! | GTGATGGAATACAAAGGTCT+TGG | + | Chr1:4097816-4097835 | MsG0180000296.01.T01:CDS | 40.0% |
| CGTTTGGATGAAGGATATGC+TGG | + | Chr1:4097873-4097892 | MsG0180000296.01.T01:CDS | 45.0% | |
| GGTTCATGGAGGTTGGAATT+TGG | + | Chr1:4097979-4097998 | MsG0180000296.01.T01:CDS | 45.0% | |
| TATGATAGCCTCACCTTCAC+CGG | - | Chr1:4097766-4097785 | None:intergenic | 45.0% | |
| ! | TCGGCTGTGATGGAATACAA+AGG | + | Chr1:4097810-4097829 | MsG0180000296.01.T01:CDS | 45.0% |
| ! | TCTTGGTCTTCAAGAAGCAG+TGG | + | Chr1:4097833-4097852 | MsG0180000296.01.T01:CDS | 45.0% |
| ! | TTTCAGGTGCGTAGACACAT+TGG | - | Chr1:4097593-4097612 | None:intergenic | 45.0% |
| ACTCACCTTTGATAGGAGCC+GGG | + | Chr1:4097706-4097725 | MsG0180000296.01.T01:CDS | 50.0% | |
| GAAGGTGAGGCTATCATACG+TGG | + | Chr1:4097768-4097787 | MsG0180000296.01.T01:CDS | 50.0% | |
| GACTCACCTTTGATAGGAGC+CGG | + | Chr1:4097705-4097724 | MsG0180000296.01.T01:CDS | 50.0% | |
| GGCTATCATACGTGGCACTT+TGG | + | Chr1:4097776-4097795 | MsG0180000296.01.T01:CDS | 50.0% | |
| GTCTACGCACCTGAAACAGT+CGG | + | Chr1:4097597-4097616 | MsG0180000296.01.T01:CDS | 50.0% | |
| GTTTAGGGGATGTGCTTCTG+AGG | + | Chr1:4097953-4097972 | MsG0180000296.01.T01:CDS | 50.0% | |
| TAGTTGTGGACCGTGAAGGA+AGG | + | Chr1:4097625-4097644 | MsG0180000296.01.T01:CDS | 50.0% | |
| TCTACGCACCTGAAACAGTC+GGG | + | Chr1:4097598-4097617 | MsG0180000296.01.T01:CDS | 50.0% | |
| ! | TGCTTCTGAGGATGGGTTCA+TGG | + | Chr1:4097965-4097984 | MsG0180000296.01.T01:CDS | 50.0% |
| ! | TTCTGAGGATGGGTTCATGG+AGG | + | Chr1:4097968-4097987 | MsG0180000296.01.T01:CDS | 50.0% |
| AACAGTCGGGTGCGTAGTTG+TGG | + | Chr1:4097611-4097630 | MsG0180000296.01.T01:CDS | 55.0% | |
| AAGGTTACACGCGTAGGTCC+CGG | - | Chr1:4097727-4097746 | None:intergenic | 55.0% | |
| ACCTACGCGTGTAACCTTGC+TGG | + | Chr1:4097729-4097748 | MsG0180000296.01.T01:CDS | 55.0% | |
| ACTACGCACCCGACTGTTTC+AGG | - | Chr1:4097609-4097628 | None:intergenic | 55.0% | |
| CAGCAGCACACCTTCCTTCA+CGG | - | Chr1:4097638-4097657 | None:intergenic | 55.0% | |
| TAGGTCCCGGCTCCTATCAA+AGG | - | Chr1:4097714-4097733 | None:intergenic | 55.0% | |
| TGCGTAGTTGTGGACCGTGA+AGG | + | Chr1:4097621-4097640 | MsG0180000296.01.T01:CDS | 55.0% | |
| ! | AGGGGATGTGCTTCTGAGGA+TGG | + | Chr1:4097957-4097976 | MsG0180000296.01.T01:CDS | 55.0% |
| ! | GAGGATGGGTTCATGGAGGT+TGG | + | Chr1:4097972-4097991 | MsG0180000296.01.T01:CDS | 55.0% |
| ! | GGGGATGTGCTTCTGAGGAT+GGG | + | Chr1:4097958-4097977 | MsG0180000296.01.T01:CDS | 55.0% |
| CATTAGCCCGCCTGTTGACG+TGG | - | Chr1:4097661-4097680 | None:intergenic | 60.0% | |
| CTTGCTGGCGTCTCATGCAC+CGG | + | Chr1:4097744-4097763 | MsG0180000296.01.T01:CDS | 60.0% | |
| GCCAGCAAGGTTACACGCGT+AGG | - | Chr1:4097733-4097752 | None:intergenic | 60.0% | |
| ! | CACTTTGGCGCGAGAGGTGT+CGG | + | Chr1:4097791-4097810 | MsG0180000296.01.T01:CDS | 60.0% |
| ! | CTCATGCACCGGTGAAGGTG+AGG | + | Chr1:4097755-4097774 | MsG0180000296.01.T01:CDS | 60.0% |
| ! | TGTGCTGCTGCCACGTCAAC+AGG | + | Chr1:4097648-4097667 | MsG0180000296.01.T01:CDS | 60.0% |
| ACGTGGCACTTTGGCGCGAG+AGG | + | Chr1:4097785-4097804 | MsG0180000296.01.T01:CDS | 65.0% | |
| CGGTGCATGAGACGCCAGCA+AGG | - | Chr1:4097746-4097765 | None:intergenic | 65.0% | |
| CTGCTGCCACGTCAACAGGC+GGG | + | Chr1:4097652-4097671 | MsG0180000296.01.T01:CDS | 65.0% | |
| GCTGCTGCCACGTCAACAGG+CGG | + | Chr1:4097651-4097670 | MsG0180000296.01.T01:CDS | 65.0% | |
| ! | GGCGTCTCATGCACCGGTGA+AGG | + | Chr1:4097750-4097769 | MsG0180000296.01.T01:CDS | 65.0% |
| !! | GCGAGAGGTGTCGGCTGTGA+TGG | + | Chr1:4097800-4097819 | MsG0180000296.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 4097582 | 4098007 | 4097582 | ID=MsG0180000296.01;Name=MsG0180000296.01 |
| Chr1 | mRNA | 4097582 | 4098007 | 4097582 | ID=MsG0180000296.01.T01;Parent=MsG0180000296.01;Name=MsG0180000296.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|141 |
| Chr1 | exon | 4097582 | 4098007 | 4097582 | ID=MsG0180000296.01.T01:exon:5726;Parent=MsG0180000296.01.T01 |
| Chr1 | CDS | 4097582 | 4098007 | 4097582 | ID=MsG0180000296.01.T01:cds;Parent=MsG0180000296.01.T01 |
| Gene Sequence |
| Protein sequence |
