AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000387.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000387.01.T01 MTR_1g016630 56.439 264 23 4 1 174 1 262 2.28e-80 239
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000387.01.T01 AT3G20430 34.221 263 79 9 1 172 1 260 1.07e-27 104

Find 29 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAATACAGAAGCAATTC+AGG 0.298572 1:-5313893 MsG0180000387.01.T01:intron
CGGCGACACCGTTTCGATTA+TGG 0.307561 1:+5318504 None:intergenic
ATTCTTAATCTGTCATGAAC+AGG 0.445504 1:+5313705 None:intergenic
TGATGTTGAAGAGGGTGAAT+TGG 0.447652 1:-5318546 MsG0180000387.01.T01:CDS
AGATCATCATCATAGGAAAC+AGG 0.457123 1:+5313681 None:intergenic
ATACAGCTGTAGGTTGCCTT+GGG 0.458577 1:-5318009 MsG0180000387.01.T01:CDS
TCCTATGATGATGATCTGCT+TGG 0.462050 1:-5313675 MsG0180000387.01.T01:CDS
TATACAGCTGTAGGTTGCCT+TGG 0.468657 1:-5318010 MsG0180000387.01.T01:CDS
TGGAATTTGGGCATGGAGGG+TGG 0.498961 1:-5318623 None:intergenic
GTTTGGAATTTGGGCATGGA+GGG 0.508864 1:-5318626 None:intergenic
TCCAAGCAGATCATCATCAT+AGG 0.511312 1:+5313674 None:intergenic
CAAGATCAAGATAAATCGCC+AGG 0.511400 1:-5313747 MsG0180000387.01.T01:CDS
ATGGATGCCTGCTTAAAACT+TGG 0.535810 1:+5313867 None:intergenic
AGAAGAAGACGATGTGGAGA+TGG 0.549529 1:-5318570 MsG0180000387.01.T01:CDS
GAACAAGACCATAATCGAAA+CGG 0.575699 1:-5318512 MsG0180000387.01.T01:CDS
CGCTCAACGCAGAAATCCCA+AGG 0.577857 1:+5317993 None:intergenic
TGCATTTGCGATGCGCAAGT+AGG 0.587168 1:+5313768 None:intergenic
AAACGGTGTCGCCGCCACAC+AGG 0.597293 1:-5318495 MsG0180000387.01.T01:CDS
AGAGGAGGATTCTTCTCAAG+AGG 0.611513 1:-5313838 MsG0180000387.01.T01:CDS
GGTTTGTGATAGAGACATGT+CGG 0.623578 1:-5318063 MsG0180000387.01.T01:intron
AAGTAGGATTGTCAGAAACA+TGG 0.643430 1:+5313784 None:intergenic
GGAGATGGTTGATGTTGAAG+AGG 0.646994 1:-5318555 MsG0180000387.01.T01:CDS
GTTGAGCGATATCGTCAAAG+AGG 0.648067 1:-5317978 MsG0180000387.01.T01:intron
GAGATGGTTGATGTTGAAGA+GGG 0.657931 1:-5318554 MsG0180000387.01.T01:CDS
TACATGGTATATACAGCTGT+AGG 0.664112 1:-5318019 MsG0180000387.01.T01:CDS
CTACGAAGAAGAAGACGATG+TGG 0.665877 1:-5318576 MsG0180000387.01.T01:CDS
TGCGCACTAGAAAATGTACA+AGG 0.667493 1:+5313813 None:intergenic
GAGATTATGATCCTGTGTGG+CGG 0.728683 1:+5318484 None:intergenic
AATGAGATTATGATCCTGTG+TGG 0.779299 1:+5318481 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATTATCAATATAATTATT+CGG - Chr1:5315442-5315461 MsG0180000387.01.T01:intron 10.0%
!! TAAATAAATCATGAAATATA+AGG + Chr1:5315328-5315347 None:intergenic 10.0%
!! TATTTCATGATTTATTTAAT+AGG - Chr1:5315330-5315349 MsG0180000387.01.T01:intron 10.0%
!!! AAATGATTTTTTTAACAAAA+TGG + Chr1:5317179-5317198 None:intergenic 10.0%
!!! ATTTGAAAAAAAATAGATTT+TGG + Chr1:5317236-5317255 None:intergenic 10.0%
!!! TTCATATTAAAAAATGTTTT+AGG - Chr1:5317278-5317297 MsG0180000387.01.T01:intron 10.0%
!!! TTTAAAAAAAATAGATTTTG+AGG + Chr1:5317374-5317393 None:intergenic 10.0%
!!! TTTAACATTTTAATTTTTTC+TGG - Chr1:5316616-5316635 MsG0180000387.01.T01:intron 10.0%
!!! TTTGTTAAAAAAATCATTTT+AGG - Chr1:5317180-5317199 MsG0180000387.01.T01:intron 10.0%
!! CATGTGTATAAAATAAATAT+TGG + Chr1:5316453-5316472 None:intergenic 15.0%
!! GATATCTTATTGAAATAATA+AGG + Chr1:5315502-5315521 None:intergenic 15.0%
!! TAAAATTAATACCAGAAAAA+TGG - Chr1:5318073-5318092 MsG0180000387.01.T01:intron 15.0%
!! TGTTAAATTCTTATAAAACA+AGG + Chr1:5316603-5316622 None:intergenic 15.0%
!!! AGAGTATTTGAATTATATTT+GGG - Chr1:5315287-5315306 MsG0180000387.01.T01:intron 15.0%
!!! TAGAGTATTTGAATTATATT+TGG - Chr1:5315286-5315305 MsG0180000387.01.T01:intron 15.0%
!!! TATTTAGGAAAATTCAATTT+TGG + Chr1:5316527-5316546 None:intergenic 15.0%
!! AAATAATATTTGAATGACCT+TGG + Chr1:5318291-5318310 None:intergenic 20.0%
!! AATAATTTCCCTTTATTGAT+GGG - Chr1:5316174-5316193 MsG0180000387.01.T01:intron 20.0%
!! AATTTCATTACAGTTATAGA+AGG - Chr1:5316576-5316595 MsG0180000387.01.T01:intron 20.0%
!! AGAATTACAAAATCAGTATT+AGG + Chr1:5314821-5314840 None:intergenic 20.0%
!! AGAGTAAAGTGAAAAAAAAA+AGG + Chr1:5317884-5317903 None:intergenic 20.0%
!! AGAGTAATGATTAAATGTAA+TGG + Chr1:5314107-5314126 None:intergenic 20.0%
!! ATATCAAACAAAAAATCTTC+CGG + Chr1:5318136-5318155 None:intergenic 20.0%
!! ATCATGAAATATAAGGAATA+TGG + Chr1:5315321-5315340 None:intergenic 20.0%
!! ATCTAAGGAAAAAAACAATA+TGG + Chr1:5315749-5315768 None:intergenic 20.0%
!! ATTATACTCTTCATAAATCA+TGG - Chr1:5316978-5316997 MsG0180000387.01.T01:intron 20.0%
!! CAATAAAGGGAAATTATTAA+AGG + Chr1:5316172-5316191 None:intergenic 20.0%
!! CCAAAATTCATAACAAAAAA+TGG + Chr1:5316937-5316956 None:intergenic 20.0%
!! TAATAATTTCCCTTTATTGA+TGG - Chr1:5316173-5316192 MsG0180000387.01.T01:intron 20.0%
!! TATATGAGAGAAAAATAAAG+TGG + Chr1:5316481-5316500 None:intergenic 20.0%
!! TATTGAAATAATAAGGACTT+GGG + Chr1:5315495-5315514 None:intergenic 20.0%
!! TCTAAGGAAAAAAACAATAT+GGG + Chr1:5315748-5315767 None:intergenic 20.0%
!! TGTATTTACTTATGTCAAAA+CGG + Chr1:5314139-5314158 None:intergenic 20.0%
!! TTATTGAAATAATAAGGACT+TGG + Chr1:5315496-5315515 None:intergenic 20.0%
!! TTCAAAAAAAATCCAACAAA+CGG + Chr1:5317088-5317107 None:intergenic 20.0%
!! TTCATTTGATAAAACAGTTT+AGG + Chr1:5314494-5314513 None:intergenic 20.0%
!! TTCTTCATTATTAAGATATC+TGG + Chr1:5314523-5314542 None:intergenic 20.0%
!!! AACTGTTTTATCAAATGAAA+AGG - Chr1:5314495-5314514 MsG0180000387.01.T01:intron 20.0%
!!! ATTTGAATTATATTTGGGTT+TGG - Chr1:5315292-5315311 MsG0180000387.01.T01:intron 20.0%
!!! CCATTTTTTGTTATGAATTT+TGG - Chr1:5316934-5316953 MsG0180000387.01.T01:intron 20.0%
!!! GTTGAAATCTAAAATATTGT+TGG - Chr1:5316770-5316789 MsG0180000387.01.T01:intron 20.0%
!!! TTAAGATTTTAGTGTAAACA+TGG - Chr1:5316699-5316718 MsG0180000387.01.T01:intron 20.0%
!!! TTGGATTGATAATTTTTACT+AGG + Chr1:5317847-5317866 None:intergenic 20.0%
!!! TTGTTTTGAACTTTTTTGTT+TGG - Chr1:5315591-5315610 MsG0180000387.01.T01:intron 20.0%
!!! TTTTTTTCACTTTACTCTAA+TGG - Chr1:5317886-5317905 MsG0180000387.01.T01:intron 20.0%
! AAGAACACCAAACATAATTA+AGG + Chr1:5317413-5317432 None:intergenic 25.0%
! AAGTATCTGTTGAAATGATA+AGG + Chr1:5316056-5316075 None:intergenic 25.0%
! AATAGTAAAGGAACAATTGA+TGG - Chr1:5316669-5316688 MsG0180000387.01.T01:intron 25.0%
! AGAAAAATGGAAATTGTTCA+TGG - Chr1:5318086-5318105 MsG0180000387.01.T01:intron 25.0%
! AGAACACCAAACATAATTAA+GGG + Chr1:5317412-5317431 None:intergenic 25.0%
! AGTAATAGATAAGCATAATC+AGG + Chr1:5316291-5316310 None:intergenic 25.0%
! ATAACACATTCAGCATTATT+AGG + Chr1:5313945-5313964 None:intergenic 25.0%
! ATACAAGTAAAAGAACACAA+AGG + Chr1:5314996-5315015 None:intergenic 25.0%
! ATTATCAAACCAGTGTAAAA+AGG + Chr1:5314676-5314695 None:intergenic 25.0%
! CAATATAATTATTCGGAGTT+TGG - Chr1:5315449-5315468 MsG0180000387.01.T01:intron 25.0%
! CTAAGAACTATTGATATTTG+GGG - Chr1:5314730-5314749 MsG0180000387.01.T01:intron 25.0%
! GAAAGAGAAAAAATCATACA+TGG - Chr1:5314217-5314236 MsG0180000387.01.T01:intron 25.0%
! GATTATATTTAAGAGCAACT+GGG + Chr1:5318252-5318271 None:intergenic 25.0%
! GCTAAGAACTATTGATATTT+GGG - Chr1:5314729-5314748 MsG0180000387.01.T01:intron 25.0%
! GTACACTGTAAAAAAATCTA+AGG + Chr1:5315764-5315783 None:intergenic 25.0%
! TAAAAACAAGAGAAAGAGAA+AGG - Chr1:5313855-5313874 MsG0180000387.01.T01:CDS 25.0%
! TAACACATTCAGCATTATTA+GGG + Chr1:5313944-5313963 None:intergenic 25.0%
! TAACTGTAATGAAATTCAGA+TGG + Chr1:5316572-5316591 None:intergenic 25.0%
! TAAGAACTATTGATATTTGG+GGG - Chr1:5314731-5314750 MsG0180000387.01.T01:intron 25.0%
! TAATAATACAGAAGCAATTC+AGG - Chr1:5318359-5318378 MsG0180000387.01.T01:intron 25.0%
! TAATTAAGTGTTAAACTCAC+AGG - Chr1:5317469-5317488 MsG0180000387.01.T01:intron 25.0%
! TCAAAGTTTAAACAGAGAAT+TGG + Chr1:5316411-5316430 None:intergenic 25.0%
! TGATTATATTTAAGAGCAAC+TGG + Chr1:5318253-5318272 None:intergenic 25.0%
! TGATTATGAAATCAGTTAAG+AGG + Chr1:5314968-5314987 None:intergenic 25.0%
! TGATTCTCCTACATATATTA+GGG + Chr1:5316735-5316754 None:intergenic 25.0%
! TGCTAAGAACTATTGATATT+TGG - Chr1:5314728-5314747 MsG0180000387.01.T01:intron 25.0%
! TGTTTAAGCAGTAAAAAGTA+AGG + Chr1:5317650-5317669 None:intergenic 25.0%
! TTGATTCTCCTACATATATT+AGG + Chr1:5316736-5316755 None:intergenic 25.0%
!! ATCTAGTAGGAGTATAAATA+AGG - Chr1:5315365-5315384 MsG0180000387.01.T01:intron 25.0%
!! ATGAAAGTATATTGTTGAGT+AGG - Chr1:5316645-5316664 MsG0180000387.01.T01:intron 25.0%
!! CATTATTAGGGTTTGAAATT+GGG + Chr1:5313932-5313951 None:intergenic 25.0%
!! CATTGATTAATAATCAACCA+AGG - Chr1:5318271-5318290 MsG0180000387.01.T01:intron 25.0%
!! GATTTATGAAGAGTATAATG+TGG + Chr1:5316977-5316996 None:intergenic 25.0%
!! TAAGAATGATCGATATTTTG+TGG - Chr1:5315031-5315050 MsG0180000387.01.T01:intron 25.0%
!! TACAAAGTTGTTTCAAATTG+TGG + Chr1:5314425-5314444 None:intergenic 25.0%
!! TCTAGTAGGAGTATAAATAA+GGG - Chr1:5315366-5315385 MsG0180000387.01.T01:intron 25.0%
!! TGATTAAATGTAATGGAACA+AGG + Chr1:5314100-5314119 None:intergenic 25.0%
!! TGATTTTCATCTTGAATTCA+TGG - Chr1:5314326-5314345 MsG0180000387.01.T01:intron 25.0%
!! TGTTGAAATGATAAGGTTTT+AGG + Chr1:5316049-5316068 None:intergenic 25.0%
!! TTGTTTTCAGACATTCTAAA+TGG + Chr1:5314460-5314479 None:intergenic 25.0%
!! TTTGTGTTCTTTTACTTGTA+TGG - Chr1:5314995-5315014 MsG0180000387.01.T01:intron 25.0%
!!! AATGAATTATGTGCTTTTTC+AGG - Chr1:5314168-5314187 MsG0180000387.01.T01:intron 25.0%
!!! AATTCTTCTTATTTCTATCG+AGG - Chr1:5314842-5314861 MsG0180000387.01.T01:intron 25.0%
!!! AGACCAATTACTCTAAAATT+TGG - Chr1:5315562-5315581 MsG0180000387.01.T01:intron 25.0%
!!! ATCCCAAATTTTAGAGTAAT+TGG + Chr1:5315568-5315587 None:intergenic 25.0%
!!! ATGAACAATTTCCATTTTTC+TGG + Chr1:5318087-5318106 None:intergenic 25.0%
!!! CTGTTTTCAGCTTATTTTAA+GGG - Chr1:5314064-5314083 MsG0180000387.01.T01:intron 25.0%
!!! CTTTACTCTAATGGATTTTA+AGG - Chr1:5317895-5317914 MsG0180000387.01.T01:intron 25.0%
!!! GACCAATTACTCTAAAATTT+GGG - Chr1:5315563-5315582 MsG0180000387.01.T01:intron 25.0%
!!! TGTCCAGTTTTTTTTATGTT+CGG + Chr1:5317948-5317967 None:intergenic 25.0%
AAAACAACTGAACTTGTCTT+TGG - Chr1:5317053-5317072 MsG0180000387.01.T01:intron 30.0%
AAAACCCAACAACATTTGTA+AGG + Chr1:5315198-5315217 None:intergenic 30.0%
AAAACTGGACAAAATATAGC+TGG - Chr1:5317957-5317976 MsG0180000387.01.T01:intron 30.0%
AAACAACTGAACTTGTCTTT+GGG - Chr1:5317054-5317073 MsG0180000387.01.T01:intron 30.0%
AACACATCAGAAACTGTAAA+TGG + Chr1:5316337-5316356 None:intergenic 30.0%
AAGAATGTGAGACCAATTAT+GGG + Chr1:5315633-5315652 None:intergenic 30.0%
AATAATAAGGACTTGGGAAA+AGG + Chr1:5315489-5315508 None:intergenic 30.0%
AGATGAAAATCAACATTGCA+AGG + Chr1:5314318-5314337 None:intergenic 30.0%
AGCAAGTACAAAATTCCTAA+AGG + Chr1:5316825-5316844 None:intergenic 30.0%
AGTTTAAACAGAGAATTGGA+AGG + Chr1:5316407-5316426 None:intergenic 30.0%
ATACTCTTCATAAATCATGG+TGG - Chr1:5316981-5317000 MsG0180000387.01.T01:intron 30.0%
ATTCTTAATCTGTCATGAAC+AGG + Chr1:5318550-5318569 None:intergenic 30.0%
ATTTGTCCAATGAACGTATT+AGG - Chr1:5316885-5316904 MsG0180000387.01.T01:intron 30.0%
CAAAACCTCTCAGTAAAAAT+CGG - Chr1:5313827-5313846 MsG0180000387.01.T01:CDS 30.0%
CGATAAGAATGATACGTATT+TGG - Chr1:5314872-5314891 MsG0180000387.01.T01:intron 30.0%
CTAATACCTAATACGTTCAT+TGG + Chr1:5316894-5316913 None:intergenic 30.0%
CTGTAAATGGTTGTATTGTA+TGG + Chr1:5316324-5316343 None:intergenic 30.0%
GCAAGTACAAAATTCCTAAA+GGG + Chr1:5316824-5316843 None:intergenic 30.0%
TAAGAATGATACGTATTTGG+TGG - Chr1:5314875-5314894 MsG0180000387.01.T01:intron 30.0%
TAAGAATGTGAGACCAATTA+TGG + Chr1:5315634-5315653 None:intergenic 30.0%
TCATTATCTCTTGTAAGCAT+TGG + Chr1:5317594-5317613 None:intergenic 30.0%
TCATTTCAACAGATACTTTG+CGG - Chr1:5316058-5316077 MsG0180000387.01.T01:intron 30.0%
TGTTGAGTAGGTAATAGTAA+AGG - Chr1:5316657-5316676 MsG0180000387.01.T01:intron 30.0%
TGTTTAAACTTTGATGTTCC+TGG - Chr1:5316417-5316436 MsG0180000387.01.T01:intron 30.0%
TTAGGAGAATAGACATGTAT+TGG + Chr1:5314803-5314822 None:intergenic 30.0%
TTGTATTGTATGGTCTTCTA+GGG + Chr1:5316314-5316333 None:intergenic 30.0%
TTTCTCTCATATAAACAGAG+CGG - Chr1:5316488-5316507 MsG0180000387.01.T01:intron 30.0%
! AGAATCAAACTGAAGGTTTT+TGG - Chr1:5316748-5316767 MsG0180000387.01.T01:intron 30.0%
! ATCTAAAATATTGTTGGACG+AGG - Chr1:5316776-5316795 MsG0180000387.01.T01:intron 30.0%
! GCATTATTAGGGTTTGAAAT+TGG + Chr1:5313933-5313952 None:intergenic 30.0%
! GCTACTATCTTTTACGAAAA+AGG + Chr1:5318204-5318223 None:intergenic 30.0%
! GTTTGATAATTCTGAATCCT+CGG - Chr1:5314686-5314705 MsG0180000387.01.T01:intron 30.0%
! TCTAAAATATTGTTGGACGA+GGG - Chr1:5316777-5316796 MsG0180000387.01.T01:intron 30.0%
! TTAAGCATACAACAGTCTTT+TGG - Chr1:5318014-5318033 MsG0180000387.01.T01:CDS 30.0%
!! GATTTTAGTGTAAACATGGT+AGG - Chr1:5316703-5316722 MsG0180000387.01.T01:intron 30.0%
!! TAGATTTTGGAAAAGCTACT+TGG + Chr1:5317223-5317242 None:intergenic 30.0%
!!! GCTGTTTTCAGCTTATTTTA+AGG - Chr1:5314063-5314082 MsG0180000387.01.T01:intron 30.0%
!!! GTCCAGTTTTTTTTATGTTC+GGG + Chr1:5317947-5317966 None:intergenic 30.0%
!!! TCAATTTTGGCTTACTTTGT+TGG + Chr1:5316514-5316533 None:intergenic 30.0%
!!! TTGTTGGGTTTTTTCATAGA+TGG - Chr1:5315206-5315225 MsG0180000387.01.T01:intron 30.0%
AAAACAGGATGCATACGTAA+TGG + Chr1:5314640-5314659 None:intergenic 35.0%
AAAGGGTGGCTTACAATATT+AGG + Chr1:5316807-5316826 None:intergenic 35.0%
AACGCACCCTTAATTATGTT+TGG - Chr1:5317403-5317422 MsG0180000387.01.T01:intron 35.0%
AAGAAACCTGTCAATGTTGA+TGG + Chr1:5313902-5313921 None:intergenic 35.0%
AAGAGAAAGAGAAAGGCTTT+AGG - Chr1:5313862-5313881 MsG0180000387.01.T01:CDS 35.0%
AAGTAGGATTGTCAGAAACA+TGG + Chr1:5318471-5318490 None:intergenic 35.0%
AATACAAAGAGCTCAAGTGT+AGG - Chr1:5317253-5317272 MsG0180000387.01.T01:intron 35.0%
AATATTGTAAGCCACCCTTT+AGG - Chr1:5316807-5316826 MsG0180000387.01.T01:intron 35.0%
AATGAGATTATGATCCTGTG+TGG + Chr1:5313774-5313793 None:intergenic 35.0%
AATTAGCAGGCAAAAAGTTC+CGG - Chr1:5318114-5318133 MsG0180000387.01.T01:intron 35.0%
ACAATGCAACACACATTTAC+TGG + Chr1:5316134-5316153 None:intergenic 35.0%
ACTCATAATCCAAATTCGCA+AGG + Chr1:5315904-5315923 None:intergenic 35.0%
AGAAACCTGTCAATGTTGAT+GGG + Chr1:5313901-5313920 None:intergenic 35.0%
AGATAAGCATAATCAGGTCA+TGG + Chr1:5316285-5316304 None:intergenic 35.0%
AGATCATCATCATAGGAAAC+AGG + Chr1:5318574-5318593 None:intergenic 35.0%
AGTACAAAATTCCTAAAGGG+TGG + Chr1:5316821-5316840 None:intergenic 35.0%
AGTAGACTAAGTGTGTGTTT+GGG - Chr1:5317713-5317732 MsG0180000387.01.T01:intron 35.0%
AGTTCAGATTCTACCTGATT+TGG - Chr1:5316254-5316273 MsG0180000387.01.T01:intron 35.0%
ATACAAAGAGCTCAAGTGTA+GGG - Chr1:5317254-5317273 MsG0180000387.01.T01:intron 35.0%
ATGTAGGAGAATCAAACTGA+AGG - Chr1:5316741-5316760 MsG0180000387.01.T01:intron 35.0%
CAATGCAACACACATTTACT+GGG + Chr1:5316133-5316152 None:intergenic 35.0%
CAATTCCATATATTAGACCG+AGG - Chr1:5318040-5318059 MsG0180000387.01.T01:CDS 35.0%
CACCCGAACATAAAAAAAAC+TGG - Chr1:5317942-5317961 MsG0180000387.01.T01:intron 35.0%
CCAACCTTACAAATGTTGTT+GGG - Chr1:5315191-5315210 MsG0180000387.01.T01:intron 35.0%
CTCACAGTTGTAGAAAACAT+AGG + Chr1:5317993-5318012 None:intergenic 35.0%
GAAATGTTTCCTTGCGAATT+TGG - Chr1:5315892-5315911 MsG0180000387.01.T01:intron 35.0%
GAACAAGACCATAATCGAAA+CGG - Chr1:5313740-5313759 MsG0180000387.01.T01:CDS 35.0%
GAAGTGAAGTGATTTCTGTT+TGG - Chr1:5317788-5317807 MsG0180000387.01.T01:intron 35.0%
GAATGTGAGTCTAATCTAGT+AGG - Chr1:5315352-5315371 MsG0180000387.01.T01:intron 35.0%
GGACTAAAAGCAACTTTACT+TGG - Chr1:5317916-5317935 MsG0180000387.01.T01:intron 35.0%
GTCTAAATTCGTGTAGGATA+GGG - Chr1:5314348-5314367 MsG0180000387.01.T01:intron 35.0%
GTTGTATTGTATGGTCTTCT+AGG + Chr1:5316315-5316334 None:intergenic 35.0%
TACATGGTATATACAGCTGT+AGG - Chr1:5314233-5314252 MsG0180000387.01.T01:intron 35.0%
TACTCACCTCTTCTTAGAAA+AGG - Chr1:5316227-5316246 MsG0180000387.01.T01:intron 35.0%
TCCAACCTTACAAATGTTGT+TGG - Chr1:5315190-5315209 MsG0180000387.01.T01:intron 35.0%
TGGTTAAAATCTGCAAGGTT+GGG + Chr1:5316370-5316389 None:intergenic 35.0%
TTCATGGTCTAAATTCGTGT+AGG - Chr1:5314342-5314361 MsG0180000387.01.T01:intron 35.0%
TTTCATCGTTGTCCCATAAT+TGG - Chr1:5315618-5315637 MsG0180000387.01.T01:intron 35.0%
TTTGACGAAGCAAAATCAAG+AGG + Chr1:5314576-5314595 None:intergenic 35.0%
! AAAGGACTTTTGCATTGAGT+TGG + Chr1:5317866-5317885 None:intergenic 35.0%
! AATATTGGTGTTTATCAGCC+AGG + Chr1:5316438-5316457 None:intergenic 35.0%
! AATTTTATGTTCACCTGCCT+CGG + Chr1:5318060-5318079 None:intergenic 35.0%
! ATGCATCCTGTTTTACTTGT+AGG - Chr1:5314646-5314665 MsG0180000387.01.T01:intron 35.0%
! CGATTTTTACTGAGAGGTTT+TGG + Chr1:5313829-5313848 None:intergenic 35.0%
! CTTTGCGGATTTTCTAATCT+TGG - Chr1:5316073-5316092 MsG0180000387.01.T01:intron 35.0%
! GTAGGAGTATAAATAAGGGA+TGG - Chr1:5315370-5315389 MsG0180000387.01.T01:intron 35.0%
! GTGTTCTTTTACTTGTATGG+AGG - Chr1:5314998-5315017 MsG0180000387.01.T01:intron 35.0%
! TATTTTGTGGTGCAAGTTTG+TGG - Chr1:5315044-5315063 MsG0180000387.01.T01:intron 35.0%
! TGAACTTGTCTTTGGGTTTA+GGG - Chr1:5317061-5317080 MsG0180000387.01.T01:intron 35.0%
! TGAATGTCAGAGCATTACTT+GGG + Chr1:5314604-5314623 None:intergenic 35.0%
! TTGAATGTCAGAGCATTACT+TGG + Chr1:5314605-5314624 None:intergenic 35.0%
!! CTCTAACCTTTTCTAAGAAG+AGG + Chr1:5316236-5316255 None:intergenic 35.0%
!! GTTTCTGGTTAAAATCTGCA+AGG + Chr1:5316375-5316394 None:intergenic 35.0%
!!! CTTTTTAAAGCTGTCCGATT+TGG - Chr1:5317430-5317449 MsG0180000387.01.T01:intron 35.0%
AACCATCTAAACAGCAGTCT+TGG + Chr1:5318173-5318192 None:intergenic 40.0%
AATAAGGACTTGGGAAAAGG+AGG + Chr1:5315486-5315505 None:intergenic 40.0%
ACACAATCAAAAGAACACCG+AGG + Chr1:5314706-5314725 None:intergenic 40.0%
ATGGATGCCTGCTTAAAACT+TGG + Chr1:5318388-5318407 None:intergenic 40.0%
CAAATTGTGACGTGCCAAAT+CGG + Chr1:5317447-5317466 None:intergenic 40.0%
CAAGATCAAGATAAATCGCC+AGG - Chr1:5318505-5318524 MsG0180000387.01.T01:CDS 40.0%
CAAGTATTCGGAAGTAAGAC+AGG + Chr1:5314937-5314956 None:intergenic 40.0%
CAGTAGACTAAGTGTGTGTT+TGG - Chr1:5317712-5317731 MsG0180000387.01.T01:intron 40.0%
CATTTGTAAGGTTGGAACCA+AGG + Chr1:5315186-5315205 None:intergenic 40.0%
CCCAACAACATTTGTAAGGT+TGG + Chr1:5315194-5315213 None:intergenic 40.0%
CTGGTTAAAATCTGCAAGGT+TGG + Chr1:5316371-5316390 None:intergenic 40.0%
CTTTACGATTGTCCAAACTG+CGG - Chr1:5314374-5314393 MsG0180000387.01.T01:intron 40.0%
GAAAAAAGCAAGAGAGTTCG+AGG - Chr1:5317612-5317631 MsG0180000387.01.T01:intron 40.0%
GAAGCAAAATCAAGAGGACT+GGG + Chr1:5314570-5314589 None:intergenic 40.0%
GAGACAGCCCTAATATATGT+AGG - Chr1:5316725-5316744 MsG0180000387.01.T01:intron 40.0%
GCCACATTCCCATCAATAAA+GGG + Chr1:5316185-5316204 None:intergenic 40.0%
GGAGAACCTACAAGTAAAAC+AGG + Chr1:5314655-5314674 None:intergenic 40.0%
GGTCTAAATTCGTGTAGGAT+AGG - Chr1:5314347-5314366 MsG0180000387.01.T01:intron 40.0%
GGTTTGTGATAGAGACATGT+CGG - Chr1:5314189-5314208 MsG0180000387.01.T01:intron 40.0%
TCAAGCAGTAAGGACTTTGT+TGG - Chr1:5316847-5316866 MsG0180000387.01.T01:intron 40.0%
TCCAAGCAGATCATCATCAT+AGG + Chr1:5318581-5318600 None:intergenic 40.0%
TCCCTTTATTGATGGGAATG+TGG - Chr1:5316181-5316200 MsG0180000387.01.T01:intron 40.0%
TGATGTTGAAGAGGGTGAAT+TGG - Chr1:5313706-5313725 MsG0180000387.01.T01:CDS 40.0%
TGCCACATTCCCATCAATAA+AGG + Chr1:5316186-5316205 None:intergenic 40.0%
TGCGCACTAGAAAATGTACA+AGG + Chr1:5318442-5318461 None:intergenic 40.0%
TGTTCCATTAAGTGCATCGA+CGG - Chr1:5317687-5317706 MsG0180000387.01.T01:intron 40.0%
! AACCAAGACTGCTGTTTAGA+TGG - Chr1:5318168-5318187 MsG0180000387.01.T01:intron 40.0%
! ACAGGAACACGTTTCTTTTC+AGG + Chr1:5318532-5318551 None:intergenic 40.0%
! AGAATCCTCCTCTTTTTGCA+TGG + Chr1:5318407-5318426 None:intergenic 40.0%
! ATTGGTCTCTGAATAGTAGC+AGG + Chr1:5315550-5315569 None:intergenic 40.0%
! CAACATTGCAAGGAAGTACA+AGG + Chr1:5314308-5314327 None:intergenic 40.0%
! CGATATCGTCAAAGAGGTAT+TGG - Chr1:5314280-5314299 MsG0180000387.01.T01:intron 40.0%
! CTGAACTTGTCTTTGGGTTT+AGG - Chr1:5317060-5317079 MsG0180000387.01.T01:intron 40.0%
! GAGATGGTTGATGTTGAAGA+GGG - Chr1:5313698-5313717 MsG0180000387.01.T01:CDS 40.0%
! GTAGGTTCTCCTTTTTACAC+TGG - Chr1:5314664-5314683 MsG0180000387.01.T01:intron 40.0%
! GTGAAGTGAGTGCTGTATAA+TGG - Chr1:5315663-5315682 MsG0180000387.01.T01:intron 40.0%
! TACTTGCTGATCAAGCAGTA+AGG - Chr1:5316837-5316856 MsG0180000387.01.T01:intron 40.0%
! TCCTATGATGATGATCTGCT+TGG - Chr1:5318577-5318596 MsG0180000387.01.T01:CDS 40.0%
!! ACATTTTCTAGTGCGCATCA+GGG - Chr1:5318445-5318464 MsG0180000387.01.T01:intron 40.0%
!! AGAAAGAGAAAGGCTTTAGG+AGG - Chr1:5313865-5313884 MsG0180000387.01.T01:CDS 40.0%
!! GCATACTAAAGAAGCATTGC+TGG + Chr1:5315693-5315712 None:intergenic 40.0%
!! GTTCATGGCTTTGAATTAGC+AGG - Chr1:5318101-5318120 MsG0180000387.01.T01:intron 40.0%
!! TACATTTTCTAGTGCGCATC+AGG - Chr1:5318444-5318463 MsG0180000387.01.T01:intron 40.0%
!! TTCTAATCTTGGCTTGTCAC+TGG - Chr1:5316084-5316103 MsG0180000387.01.T01:intron 40.0%
ACCTGCCTCGGTCTAATATA+TGG + Chr1:5318048-5318067 None:intergenic 45.0%
AGAAGAAGACGATGTGGAGA+TGG - Chr1:5313682-5313701 MsG0180000387.01.T01:CDS 45.0%
AGAGGAGGATTCTTCTCAAG+AGG - Chr1:5318414-5318433 MsG0180000387.01.T01:intron 45.0%
AGTAGCAGGACAAGTCACAA+AGG + Chr1:5315536-5315555 None:intergenic 45.0%
ATACAGCTGTAGGTTGCCTT+GGG - Chr1:5314243-5314262 MsG0180000387.01.T01:intron 45.0%
ATGCACCCATCAACATTGAC+AGG - Chr1:5313893-5313912 MsG0180000387.01.T01:intron 45.0%
ATGGTAAGCGATTTCGGACA+GGG - Chr1:5317531-5317550 MsG0180000387.01.T01:intron 45.0%
CAGCACTCACTTCACTTCAA+TGG + Chr1:5315658-5315677 None:intergenic 45.0%
CGAAGCAAAATCAAGAGGAC+TGG + Chr1:5314571-5314590 None:intergenic 45.0%
CTACGAAGAAGAAGACGATG+TGG - Chr1:5313676-5313695 MsG0180000387.01.T01:CDS 45.0%
CTGCTGATGGTAAGCGATTT+CGG - Chr1:5317525-5317544 MsG0180000387.01.T01:intron 45.0%
GAGATTATGATCCTGTGTGG+CGG + Chr1:5313771-5313790 None:intergenic 45.0%
GATATTTGGGGGTGAGTTTG+AGG - Chr1:5314742-5314761 MsG0180000387.01.T01:intron 45.0%
GTTGAGCGATATCGTCAAAG+AGG - Chr1:5314274-5314293 MsG0180000387.01.T01:intron 45.0%
TAAGGTTGGAACCAAGGTTG+AGG + Chr1:5315180-5315199 None:intergenic 45.0%
TAGCCACTTCAACCTCTCTT+GGG - Chr1:5315147-5315166 MsG0180000387.01.T01:intron 45.0%
TATACAGCTGTAGGTTGCCT+TGG - Chr1:5314242-5314261 MsG0180000387.01.T01:intron 45.0%
TCCATATATTAGACCGAGGC+AGG - Chr1:5318044-5318063 MsG0180000387.01.T01:CDS 45.0%
TGGGAATGTGGCATTGTTTG+AGG - Chr1:5316193-5316212 MsG0180000387.01.T01:intron 45.0%
TGTGGTGTTTGCCTTGTGTA+TGG - Chr1:5315062-5315081 MsG0180000387.01.T01:intron 45.0%
TTACTGGGAGATGAAGAGAG+AGG + Chr1:5316118-5316137 None:intergenic 45.0%
! ACTGCTGTTTAGATGGTTCC+GGG - Chr1:5318175-5318194 MsG0180000387.01.T01:intron 45.0%
! CGCTTCCGATTTTTACTGAG+AGG + Chr1:5313835-5313854 None:intergenic 45.0%
! CTTTTACGAAAAAGGCAGCC+CGG + Chr1:5318196-5318215 None:intergenic 45.0%
! GACTGCTGTTTAGATGGTTC+CGG - Chr1:5318174-5318193 MsG0180000387.01.T01:intron 45.0%
! GAGGAGCACATGCAAGTATT+CGG + Chr1:5314949-5314968 None:intergenic 45.0%
! GGAAGGAGCAAACTAGTTTC+TGG + Chr1:5316390-5316409 None:intergenic 45.0%
! GGAGATGGTTGATGTTGAAG+AGG - Chr1:5313697-5313716 MsG0180000387.01.T01:CDS 45.0%
! GTCTTTGCACTCACGTTTTG+TGG - Chr1:5315720-5315739 MsG0180000387.01.T01:intron 45.0%
!! AAGGAACAATTGATGGCGCT+AGG - Chr1:5316676-5316695 MsG0180000387.01.T01:intron 45.0%
!! ACACGTTTCTTTTCAGGACC+TGG + Chr1:5318526-5318545 None:intergenic 45.0%
!! GAGAAAGGCTTTAGGAGGAT+CGG - Chr1:5313870-5313889 MsG0180000387.01.T01:CDS 45.0%
!!! GATAAGGTTTTAGGCGCAGA+AGG + Chr1:5316040-5316059 None:intergenic 45.0%
!! AATAAAAATATGATATATTT+AGG + Chr1:5316542-5316561 None:intergenic 5.0%
AGCCACTTCAACCTCTCTTG+GGG - Chr1:5315148-5315167 MsG0180000387.01.T01:intron 50.0%
CGTGAGTGCAAAGACAGATG+AGG + Chr1:5315715-5315734 None:intergenic 50.0%
GATGGTAAGCGATTTCGGAC+AGG - Chr1:5317530-5317549 MsG0180000387.01.T01:intron 50.0%
GCAGAGAACCACCATACACA+AGG + Chr1:5315076-5315095 None:intergenic 50.0%
GCAGGACAAGTCACAAAGGA+AGG + Chr1:5315532-5315551 None:intergenic 50.0%
GCAGGCATCCATGCAAAAAG+AGG - Chr1:5318396-5318415 MsG0180000387.01.T01:intron 50.0%
GGACTTGGGAAAAGGAGGAA+AGG + Chr1:5315481-5315500 None:intergenic 50.0%
GGCATCCATGCAAAAAGAGG+AGG - Chr1:5318399-5318418 MsG0180000387.01.T01:intron 50.0%
GGTCATGGCTGAACCAAATC+AGG + Chr1:5316270-5316289 None:intergenic 50.0%
GGTGTTTGCCTTGTGTATGG+TGG - Chr1:5315065-5315084 MsG0180000387.01.T01:intron 50.0%
GTAGACGCAATACAGTCCTG+TGG - Chr1:5317491-5317510 MsG0180000387.01.T01:intron 50.0%
GTAGCCACTTCAACCTCTCT+TGG - Chr1:5315146-5315165 MsG0180000387.01.T01:intron 50.0%
TGCATTTGCGATGCGCAAGT+AGG + Chr1:5318487-5318506 None:intergenic 50.0%
TGGTAAGCGATTTCGGACAG+GGG - Chr1:5317532-5317551 MsG0180000387.01.T01:intron 50.0%
TGTGCCGTCGATGCACTTAA+TGG + Chr1:5317694-5317713 None:intergenic 50.0%
TTGCAATTGCAGCCGCAGTT+TGG + Chr1:5314389-5314408 None:intergenic 50.0%
! TGGGTTTAGGGTCCGTTTGT+TGG - Chr1:5317073-5317092 MsG0180000387.01.T01:intron 50.0%
!!! CAGGCAGCCAAGTTTTAAGC+AGG - Chr1:5318378-5318397 MsG0180000387.01.T01:intron 50.0%
!!! GGTTTTAGGCGCAGAAGGAA+AGG + Chr1:5316035-5316054 None:intergenic 50.0%
CACCCCAAGAGAGGTTGAAG+TGG + Chr1:5315153-5315172 None:intergenic 55.0%
CACTTCAACCTCTCTTGGGG+TGG - Chr1:5315151-5315170 MsG0180000387.01.T01:intron 55.0%
CGCTCAACGCAGAAATCCCA+AGG + Chr1:5314262-5314281 None:intergenic 55.0%
CGGCGACACCGTTTCGATTA+TGG + Chr1:5313751-5313770 None:intergenic 55.0%
GACGCAATACAGTCCTGTGG+AGG - Chr1:5317494-5317513 MsG0180000387.01.T01:intron 55.0%
GCTTACCATCAGCAGTCTGC+TGG + Chr1:5317520-5317539 None:intergenic 55.0%
GGTAAGCGATTTCGGACAGG+GGG - Chr1:5317533-5317552 MsG0180000387.01.T01:intron 55.0%
TGAGGAAACCACCCCAAGAG+AGG + Chr1:5315162-5315181 None:intergenic 55.0%
TGGGGTGGTTTCCTCAACCT+TGG - Chr1:5315166-5315185 MsG0180000387.01.T01:intron 55.0%
AAGCGATTTCGGACAGGGGG+TGG - Chr1:5317536-5317555 MsG0180000387.01.T01:intron 60.0%
GGACAGGGGGTGGTGTATTG+TGG - Chr1:5317546-5317565 MsG0180000387.01.T01:intron 60.0%
GGAGGCCAGCAGACTGCTGA+TGG - Chr1:5317512-5317531 MsG0180000387.01.T01:intron 65.0%
! AGCAGTCTGCTGGCCTCCAC+AGG + Chr1:5317510-5317529 None:intergenic 65.0%
!! AAACGGTGTCGCCGCCACAC+AGG - Chr1:5313757-5313776 MsG0180000387.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5313641 5318633 5313641 ID=MsG0180000387.01;Name=MsG0180000387.01
Chr1 mRNA 5313641 5318633 5313641 ID=MsG0180000387.01.T01;Parent=MsG0180000387.01;Name=MsG0180000387.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|174
Chr1 exon 5318468 5318633 5318468 ID=MsG0180000387.01.T01:exon:2750;Parent=MsG0180000387.01.T01
Chr1 exon 5317979 5318073 5317979 ID=MsG0180000387.01.T01:exon:2751;Parent=MsG0180000387.01.T01
Chr1 exon 5313641 5313904 5313641 ID=MsG0180000387.01.T01:exon:2752;Parent=MsG0180000387.01.T01
Chr1 CDS 5318468 5318633 5318468 ID=MsG0180000387.01.T01:cds;Parent=MsG0180000387.01.T01
Chr1 CDS 5317979 5318073 5317979 ID=MsG0180000387.01.T01:cds;Parent=MsG0180000387.01.T01
Chr1 CDS 5313641 5313904 5313641 ID=MsG0180000387.01.T01:cds;Parent=MsG0180000387.01.T01
Gene Sequence

>MsG0180000387.01.T01

ATGGAGGGTGGTGAGAACATTCTAGATGCTATTAACTACGAAGAAGAAGACGATGTGGAGATGGTTGATGTTGAAGAGGGTGAATTGGTTGAAGAAGAAGAACAAGACCATAATCGAAACGGTGTCGCCGCCACACAGGATCATAATCTCATTCATGAATCAGATAAGACATGTCGGCGTCTGAAAGAGAAAAAATCATACATGGTATATACAGCTGTAGGTTGCCTTGGGATTTCTGCGTTGAGCGATATCGTCAAAGAGAAGCAATTCAGGCAGCCAAGTTTTAAGCAGGCATCCATGCAAAAAGAGGAGGATTCTTCTCAAGAGGTGCCTTGTACATTTTCTAGTGCGCATCAGGGCCATGTTTCTGACAATCCTACTTGCGCATCGCAAATGCAAGATCAAGATAAATCGCCAGGTCCTGAAAAGAAACGTGTTCCTGTTCATGACAGATTAAGAATACCTGTTTCCTATGATGATGATCTGCTTGGAGCAAGCGTTGATAATGATGTAACCTTGCCTTGA
Protein sequence

>MsG0180000387.01.T01

MEGGENILDAINYEEEDDVEMVDVEEGELVEEEEQDHNRNGVAATQDHNLIHESDKTCRRLKEKKSYMVYTAVGCLGISALSDIVKEKQFRQPSFKQASMQKEEDSSQEVPCTFSSAHQGHVSDNPTCASQMQDQDKSPGPEKKRVPVHDRLRIPVSYDDDLLGASVDNDVTLP*