AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000388.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000388.01.T01 MTR_1g016780 92.655 177 12 1 1 177 99 274 2.98e-100 298
MsG0180000388.01.T01 MTR_3g104120 77.401 177 39 1 1 177 90 265 4.90e-92 276
MsG0180000388.01.T01 MTR_4g101730 64.407 177 62 1 1 177 100 275 9.92e-75 229
MsG0180000388.01.T01 MTR_4g101730 64.407 177 62 1 1 177 100 275 1.21e-73 229
MsG0180000388.01.T01 MTR_7g032230 60.452 177 69 1 1 177 75 250 8.45e-68 214
MsG0180000388.01.T01 MTR_7g079140 56.425 179 73 2 1 177 88 263 4.02e-62 199
MsG0180000388.01.T01 MTR_7g075310 52.514 179 80 2 1 177 87 262 7.18e-57 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000388.01.T01 AT2G25940 75.706 177 42 1 1 177 85 260 3.50e-88 266
MsG0180000388.01.T01 AT4G32940 74.576 177 44 1 1 177 100 275 9.33e-80 245
MsG0180000388.01.T01 AT1G62710 65.537 177 60 1 1 177 92 267 3.04e-76 236
MsG0180000388.01.T01 AT3G20210 61.017 177 68 1 1 177 87 262 5.17e-62 199

Find 89 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTGGTTCTAATGGTTAT+TGG 0.191155 1:+5331199 MsG0180000388.01.T01:five_prime_UTR
CTAATGGTTATTGGAATTAT+AGG 0.241588 1:+5331208 MsG0180000388.01.T01:five_prime_UTR
GGAAATAAATCAGCTCTTAC+AGG 0.282325 1:+5333245 MsG0180000388.01.T01:CDS
AATTTCTATGCTGCTTTACT+TGG 0.313296 1:+5333224 MsG0180000388.01.T01:CDS
TATCTGGAGGCATGTGAATC+TGG 0.334996 1:+5334147 MsG0180000388.01.T01:CDS
CATGGAGGTCCAGGGGTTCT+TGG 0.340017 1:+5333328 MsG0180000388.01.T01:CDS
GTATACAAAATATGATCATT+TGG 0.342499 1:-5333296 None:intergenic
CATTCTCCTTCCTTCAATCA+TGG 0.352942 1:+5330995 MsG0180000388.01.T01:five_prime_UTR
AGAGAAGATTTAAATAATAA+AGG 0.366081 1:-5330951 None:intergenic
GCGTAGATATTGATATCTTC+TGG 0.396831 1:-5334192 None:intergenic
ACCATCTTTGCCGCTGTCTC+CGG 0.401354 1:+5331061 MsG0180000388.01.T01:five_prime_UTR
CCTTCCAAGCAATGCTGTAT+AGG 0.407392 1:-5334311 None:intergenic
TTTGTATACTACACTGATCA+TGG 0.422361 1:+5333310 MsG0180000388.01.T01:CDS
TATACAAAATATGATCATTT+GGG 0.423371 1:-5333295 None:intergenic
CATTGCTTGGAAGGGAAGAC+AGG 0.437205 1:+5334320 MsG0180000388.01.T01:CDS
TTAATTATGACTCCAGGCCT+TGG 0.438134 1:-5332946 None:intergenic
GTCTCTTCAGGTATGCCAGT+TGG 0.457359 1:+5333838 MsG0180000388.01.T01:intron
TACACTGATCATGGAGGTCC+AGG 0.458300 1:+5333319 MsG0180000388.01.T01:CDS
GTCATGCGTATCAATTATTG+AGG 0.462318 1:+5332854 MsG0180000388.01.T01:five_prime_UTR
CCTATACAGCATTGCTTGGA+AGG 0.475368 1:+5334311 MsG0180000388.01.T01:CDS
TGGCAATCGGATATAATCTC+CGG 0.476800 1:-5331097 None:intergenic
GAAAATGATGACAACGTTGA+AGG 0.477273 1:+5331156 MsG0180000388.01.T01:five_prime_UTR
CATCTCAATCTCAAGCCTCC+AGG 0.478973 1:+5331118 MsG0180000388.01.T01:five_prime_UTR
CTCCGGGGAGTCACGGCTAC+CGG 0.481324 1:-5331080 None:intergenic
CCAGAGTACTCAACTTGCTT+AGG 0.481337 1:+5334285 MsG0180000388.01.T01:CDS
GCATACAAGTAAGGACCAAC+TGG 0.486355 1:-5333853 None:intergenic
TTATTGGAATTATAGGCATC+AGG 0.488640 1:+5331215 MsG0180000388.01.T01:five_prime_UTR
GTGACCTATACAGCATTGCT+TGG 0.490888 1:+5334307 MsG0180000388.01.T01:CDS
TCATAAACATCACCACCATC+AGG 0.496461 1:-5332973 None:intergenic
GACGGTAAGAAAGAGGATAG+TGG 0.497500 1:-5331033 None:intergenic
GTTGAGTACTCTGGAGGAGG+GGG 0.498780 1:-5334276 None:intergenic
TGAGAAAAGTCCATGATTGA+AGG 0.499589 1:-5331005 None:intergenic
AGATCAGATGCATACAAGTA+AGG 0.499897 1:-5333862 None:intergenic
AAATGCAGTAGAAAGCAGTT+GGG 0.502368 1:+5334227 MsG0180000388.01.T01:CDS
CTCCGGTAGCCGTGACTCCC+CGG 0.504790 1:+5331078 MsG0180000388.01.T01:five_prime_UTR
CAAATGCAGTAGAAAGCAGT+TGG 0.506782 1:+5334226 MsG0180000388.01.T01:CDS
GAATCTGGCAGTATCTTTGA+AGG 0.512925 1:+5334162 MsG0180000388.01.T01:CDS
ACCGGAGACAGCGGCAAAGA+TGG 0.514404 1:-5331062 None:intergenic
GGTTTGTTAATTATGACTCC+AGG 0.517005 1:-5332952 None:intergenic
TTCAGGTTCATGGAAGAACC+TGG 0.517884 1:-5331136 None:intergenic
GGCAATCGGATATAATCTCC+GGG 0.519142 1:-5331096 None:intergenic
AGTTGGGGAACATATTGCCC+TGG 0.526331 1:+5334243 MsG0180000388.01.T01:CDS
CTTGAGATTGAGATGGCAAT+CGG 0.526844 1:-5331110 None:intergenic
ATATAATCTCCGGGGAGTCA+CGG 0.528606 1:-5331087 None:intergenic
TCAGCTCTTACAGGTGGGAG+TGG 0.533587 1:+5333254 MsG0180000388.01.T01:CDS
CAAGTTGAGTACTCTGGAGG+AGG 0.540236 1:-5334279 None:intergenic
AATGTAGAGAATCCAAGGCC+TGG 0.541898 1:+5332934 MsG0180000388.01.T01:CDS
AAAATGATGACAACGTTGAA+GGG 0.542555 1:+5331157 MsG0180000388.01.T01:five_prime_UTR
CCTAAGCAAGTTGAGTACTC+TGG 0.544668 1:-5334285 None:intergenic
GTTGGGGAACATATTGCCCT+GGG 0.545583 1:+5334244 MsG0180000388.01.T01:CDS
GTAAGATGACGGTAAGAAAG+AGG 0.555102 1:-5331040 None:intergenic
AGTTGAGTACTCTGGAGGAG+GGG 0.559521 1:-5334277 None:intergenic
AGGCCTTGGATTCTCTACAT+TGG 0.562654 1:-5332932 None:intergenic
AGTGGGAAAGTTGTTGATAG+TGG 0.565083 1:+5333272 MsG0180000388.01.T01:CDS
GCGTATCAATTATTGAGGAA+TGG 0.567662 1:+5332859 MsG0180000388.01.T01:five_prime_UTR
AGGAGAATGAATGAGTGAAG+AGG 0.569468 1:-5330981 None:intergenic
TTCTTATGACAGGATTACAC+TGG 0.570349 1:+5333182 MsG0180000388.01.T01:intron
GTCATAATTAACAAACCTGA+TGG 0.573212 1:+5332958 MsG0180000388.01.T01:CDS
AATTACTCACCAAGAACCCC+TGG 0.573730 1:-5333337 None:intergenic
AAGTTGAGTACTCTGGAGGA+GGG 0.582976 1:-5334278 None:intergenic
CTTCCAATGTAGAGAATCCA+AGG 0.584961 1:+5332929 MsG0180000388.01.T01:CDS
AATAAATCAGCTCTTACAGG+TGG 0.589532 1:+5333248 MsG0180000388.01.T01:CDS
AAAAGTCCATGATTGAAGGA+AGG 0.592337 1:-5331001 None:intergenic
ACACTGATCATGGAGGTCCA+GGG 0.595033 1:+5333320 MsG0180000388.01.T01:CDS
CAGCTCTTACAGGTGGGAGT+GGG 0.602741 1:+5333255 MsG0180000388.01.T01:CDS
CTATACAGCATTGCTTGGAA+GGG 0.611429 1:+5334312 MsG0180000388.01.T01:CDS
CTGGAGGCTTGAGATTGAGA+TGG 0.612586 1:-5331117 None:intergenic
TATCAATTATTGAGGAATGG+TGG 0.612607 1:+5332862 MsG0180000388.01.T01:five_prime_UTR
ATGACAACGTTGAAGGGACT+AGG 0.618129 1:+5331163 MsG0180000388.01.T01:five_prime_UTR
CACTGATCATGGAGGTCCAG+GGG 0.620699 1:+5333321 MsG0180000388.01.T01:CDS
ATTTAAATAATAAAGGTGAG+AGG 0.628502 1:-5330944 None:intergenic
TGTTTATGAAGGAGTTCCAA+AGG 0.634134 1:+5332987 MsG0180000388.01.T01:CDS
CGGCAAAGATGGTAAGATGA+CGG 0.635536 1:-5331051 None:intergenic
ACAACGTTGAAGGGACTAGG+TGG 0.636966 1:+5331166 MsG0180000388.01.T01:five_prime_UTR
ATAAATCAGCTCTTACAGGT+GGG 0.637399 1:+5333249 MsG0180000388.01.T01:CDS
ATTGCTTGGAAGGGAAGACA+GGG 0.637456 1:+5334321 MsG0180000388.01.T01:CDS
TATCAATATCTACGCGACAA+CGG 0.638805 1:+5334200 MsG0180000388.01.T01:CDS
GATGGTGGTGATGTTTATGA+AGG 0.641773 1:+5332976 MsG0180000388.01.T01:CDS
GTCACGGCTACCGGAGACAG+CGG 0.645729 1:-5331071 None:intergenic
AAGGTGAGAGGTAGTTGCAG+TGG 0.649923 1:-5330932 None:intergenic
ATAATTAACAAACCTGATGG+TGG 0.650511 1:+5332961 MsG0180000388.01.T01:CDS
CAACGTTGAAGGGACTAGGT+GGG 0.651451 1:+5331167 MsG0180000388.01.T01:five_prime_UTR
ACAGGATTACACTGGTGCAG+AGG 0.651826 1:+5333190 MsG0180000388.01.T01:intron
AATGCAGTAGAAAGCAGTTG+GGG 0.656559 1:+5334228 MsG0180000388.01.T01:CDS
AAGCAAGTTGAGTACTCTGG+AGG 0.665445 1:-5334282 None:intergenic
AGGTTCATGGAAGAACCTGG+AGG 0.710107 1:-5331133 None:intergenic
GTATACTACACTGATCATGG+AGG 0.754047 1:+5333313 MsG0180000388.01.T01:CDS
TTGGGGAACATATTGCCCTG+GGG 0.755738 1:+5334245 MsG0180000388.01.T01:CDS
GCAATCGGATATAATCTCCG+GGG 0.768326 1:-5331095 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATTTGTCTATTTAAT+TGG - Chr1:5331361-5331380 None:intergenic 10.0%
!! TATTTCAAAATTGTATTTAT+TGG + Chr1:5333742-5333761 MsG0180000388.01.T01:intron 10.0%
!! TTTGTATTATAAAAAAATCA+AGG + Chr1:5331993-5332012 MsG0180000388.01.T01:intron 10.0%
!!! ATACATAAACTTTTTTTTAA+AGG + Chr1:5331270-5331289 MsG0180000388.01.T01:intron 10.0%
!! AGAGAAGATTTAAATAATAA+AGG - Chr1:5330954-5330973 None:intergenic 15.0%
!! GAAAACTAAAAAAAACATAA+GGG + Chr1:5332233-5332252 MsG0180000388.01.T01:intron 15.0%
!! GAAAACTAAAATAAACATAA+GGG - Chr1:5332184-5332203 None:intergenic 15.0%
!! GTAAAAAAAAAAAAAACTCA+AGG - Chr1:5331511-5331530 None:intergenic 15.0%
!! TATACAAAATATGATCATTT+GGG - Chr1:5333298-5333317 None:intergenic 15.0%
!! TGAAAACTAAAAAAAACATA+AGG + Chr1:5332232-5332251 MsG0180000388.01.T01:intron 15.0%
!! TGAAAACTAAAATAAACATA+AGG - Chr1:5332185-5332204 None:intergenic 15.0%
!!! AATTTTTTTGACTTATGTAA+TGG + Chr1:5334110-5334129 MsG0180000388.01.T01:intron 15.0%
!!! CAAATAAATACTTTTTTTGA+AGG + Chr1:5331371-5331390 MsG0180000388.01.T01:intron 15.0%
!! AATGAAAATAGTTGAATGTA+TGG + Chr1:5332638-5332657 MsG0180000388.01.T01:intron 20.0%
!! ACTGAAAACTAAATAACATA+AGG + Chr1:5332347-5332366 MsG0180000388.01.T01:intron 20.0%
!! ACTTGAAACTAAAATAACAT+AGG - Chr1:5332038-5332057 None:intergenic 20.0%
!! ATAAACTAATAATACAAGTC+TGG + Chr1:5331604-5331623 MsG0180000388.01.T01:intron 20.0%
!! ATGAAAATAGTTGAATGTAT+GGG + Chr1:5332639-5332658 MsG0180000388.01.T01:intron 20.0%
!! ATTGAAAAACAATTATGTAC+AGG + Chr1:5332668-5332687 MsG0180000388.01.T01:intron 20.0%
!! ATTTAAATAATAAAGGTGAG+AGG - Chr1:5330947-5330966 None:intergenic 20.0%
!! CTGAAAACTAAATAACATAA+GGG + Chr1:5332348-5332367 MsG0180000388.01.T01:intron 20.0%
!! CTTGAAACTAAAATAACATA+GGG - Chr1:5332037-5332056 None:intergenic 20.0%
!! GAAAACCAAAATAAACATAA+GGG - Chr1:5332153-5332172 None:intergenic 20.0%
!! GAAATAATTAAATCCAAACA+TGG - Chr1:5334007-5334026 None:intergenic 20.0%
!! GTATACAAAATATGATCATT+TGG - Chr1:5333299-5333318 None:intergenic 20.0%
!! TGAAAACCAAAATAAACATA+AGG - Chr1:5332154-5332173 None:intergenic 20.0%
!! TGTTTGGATTTAATTATTTC+AGG + Chr1:5334007-5334026 MsG0180000388.01.T01:intron 20.0%
!! TTGAAAAACAATTATGTACA+GGG + Chr1:5332669-5332688 MsG0180000388.01.T01:intron 20.0%
!! TTGAATATTATCACATGTAT+CGG - Chr1:5333499-5333518 None:intergenic 20.0%
!!! ACATTCAACTATTTTCATTT+TGG - Chr1:5332637-5332656 None:intergenic 20.0%
!!! ACATTTTCATTAATCTGTAT+TGG - Chr1:5331725-5331744 None:intergenic 20.0%
!!! ACTTATAATTTAAAGTCAAC+TGG + Chr1:5332576-5332595 MsG0180000388.01.T01:intron 20.0%
!!! ATCTGAGTTTTTATTTTTGT+CGG + Chr1:5331539-5331558 MsG0180000388.01.T01:intron 20.0%
!!! ATGATTTTACCTATTTATGT+TGG - Chr1:5331420-5331439 None:intergenic 20.0%
!!! ATGTTATTTTAGTTTCAAGT+TGG + Chr1:5332038-5332057 MsG0180000388.01.T01:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG + Chr1:5332188-5332207 MsG0180000388.01.T01:intron 20.0%
!!! GTTTTTTTTAGTTTTCAAGT+CGG - Chr1:5332229-5332248 None:intergenic 20.0%
!!! TATGTTATTTAGTTTTCAGT+TGG - Chr1:5332347-5332366 None:intergenic 20.0%
!!! TGAAACTTTTTAAAACGTAA+GGG + Chr1:5332292-5332311 MsG0180000388.01.T01:intron 20.0%
!!! TTGAAACTTTTTAAAACGTA+AGG + Chr1:5332291-5332310 MsG0180000388.01.T01:intron 20.0%
! AAATCATGAAGTGATTTGAT+TGG + Chr1:5331433-5331452 MsG0180000388.01.T01:intron 25.0%
! AATTATGTACAGGGATTAAA+TGG + Chr1:5332678-5332697 MsG0180000388.01.T01:intron 25.0%
! ACAATAAACACACTATTTGT+CGG - Chr1:5333651-5333670 None:intergenic 25.0%
! AGACCAAATAAGAAATAGAA+AGG - Chr1:5333822-5333841 None:intergenic 25.0%
! AGAGACCAAATTAAAATCAA+GGG - Chr1:5331634-5331653 None:intergenic 25.0%
! AGATAATGTTGAATTTCAGA+AGG + Chr1:5332715-5332734 MsG0180000388.01.T01:intron 25.0%
! AGCTATTGAATGTAACAATA+TGG - Chr1:5333088-5333107 None:intergenic 25.0%
! CTAATGGTTATTGGAATTAT+AGG + Chr1:5331208-5331227 MsG0180000388.01.T01:five_prime_UTR 25.0%
! TAAGTTGTTTGTATGTGTTT+AGG + Chr1:5333590-5333609 MsG0180000388.01.T01:intron 25.0%
! TAGAGACCAAATTAAAATCA+AGG - Chr1:5331635-5331654 None:intergenic 25.0%
! TGAAAATAGTTGAATGTATG+GGG + Chr1:5332640-5332659 MsG0180000388.01.T01:intron 25.0%
! TTGAAACTTTCAAAACGTAA+GGG + Chr1:5332262-5332281 MsG0180000388.01.T01:intron 25.0%
!! AAACTTTTTGATGAAAGACA+TGG + Chr1:5333377-5333396 MsG0180000388.01.T01:intron 25.0%
!! ACTAAAATTGACTTTACCTT+TGG - Chr1:5333006-5333025 None:intergenic 25.0%
!! ATATTTCATTCCAACTGTTT+TGG - Chr1:5333725-5333744 None:intergenic 25.0%
!! CTTTAGAAACCAACATAAAT+AGG + Chr1:5331408-5331427 MsG0180000388.01.T01:intron 25.0%
!! GTTCCTTTCTATTTCTTATT+TGG + Chr1:5333816-5333835 MsG0180000388.01.T01:intron 25.0%
!! TTAGTCCCTTATGTTTATTT+TGG + Chr1:5332145-5332164 MsG0180000388.01.T01:intron 25.0%
!! TTTGATTGAATTGTGTTTTG+TGG + Chr1:5333679-5333698 MsG0180000388.01.T01:intron 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG + Chr1:5332095-5332114 MsG0180000388.01.T01:intron 25.0%
!!! CGTTTTAAAAAGTTTCAAGT+TGG - Chr1:5332289-5332308 None:intergenic 25.0%
!!! CTGATATACATGACTTTTTT+TGG - Chr1:5331775-5331794 None:intergenic 25.0%
!!! GTTATCCCTTGATTTTAATT+TGG + Chr1:5331626-5331645 MsG0180000388.01.T01:intron 25.0%
!!! GTTTATTTTGGTTTTCAAGT+TGG + Chr1:5332157-5332176 MsG0180000388.01.T01:intron 25.0%
!!! TCTGAGTTTTTATTTTTGTC+GGG + Chr1:5331540-5331559 MsG0180000388.01.T01:intron 25.0%
AAAATGATGACAACGTTGAA+GGG + Chr1:5331157-5331176 MsG0180000388.01.T01:five_prime_UTR 30.0%
AATTTGGTCTCTATCAGAAT+TGG + Chr1:5331642-5331661 MsG0180000388.01.T01:intron 30.0%
ACTATTACTAGTGACTTACT+AGG - Chr1:5333933-5333952 None:intergenic 30.0%
ACTTGAAACTTTCAAACGTA+AGG + Chr1:5332319-5332338 MsG0180000388.01.T01:intron 30.0%
ACTTGAAACTTTCAAACGTA+AGG - Chr1:5332338-5332319 None:intergenic 30.0%
AGTAACATCATCAAAGACAA+AGG - Chr1:5331482-5331501 None:intergenic 30.0%
ATAATTAACAAACCTGATGG+TGG + Chr1:5332961-5332980 MsG0180000388.01.T01:CDS 30.0%
ATTGACTAATCCTAATCTGA+AGG - Chr1:5331938-5331957 None:intergenic 30.0%
CGTATATGTTTCACATTCAT+TGG + Chr1:5331809-5331828 MsG0180000388.01.T01:intron 30.0%
CTTGAAACTTTCAAAACGTA+AGG + Chr1:5332261-5332280 MsG0180000388.01.T01:intron 30.0%
CTTGAAACTTTCAAACGTAA+GGG + Chr1:5332320-5332339 MsG0180000388.01.T01:intron 30.0%
CTTGAAACTTTCAAACGTAA+GGG - Chr1:5332339-5332320 None:intergenic 30.0%
GTCATAATTAACAAACCTGA+TGG + Chr1:5332958-5332977 MsG0180000388.01.T01:CDS 30.0%
TAACACTAACATATCGACAT+TGG - Chr1:5333546-5333565 None:intergenic 30.0%
TATCAATTATTGAGGAATGG+TGG + Chr1:5332862-5332881 MsG0180000388.01.T01:five_prime_UTR 30.0%
TTATTGGAATTATAGGCATC+AGG + Chr1:5331215-5331234 MsG0180000388.01.T01:five_prime_UTR 30.0%
TTGAAGAAAAAACATGCTTC+TGG + Chr1:5333895-5333914 MsG0180000388.01.T01:CDS 30.0%
TTGACTAATCCTAATCTGAA+GGG - Chr1:5331937-5331956 None:intergenic 30.0%
TTTGTATACTACACTGATCA+TGG + Chr1:5333310-5333329 MsG0180000388.01.T01:CDS 30.0%
! AGCTTGCATAATAAACCTTT+TGG + Chr1:5334051-5334070 MsG0180000388.01.T01:intron 30.0%
! ATTTCTTATTTGGTCTCTTC+AGG + Chr1:5333826-5333845 MsG0180000388.01.T01:intron 30.0%
! TACGTTTGAAAGTTTCAAGT+TGG + Chr1:5332066-5332085 MsG0180000388.01.T01:intron 30.0%
! TACGTTTGAAAGTTTCAAGT+TGG - Chr1:5332085-5332066 None:intergenic 30.0%
!! AATTTCTATGCTGCTTTACT+TGG + Chr1:5333224-5333243 MsG0180000388.01.T01:CDS 30.0%
!! TAAGAGCTTTTTCTTATGAC+AGG + Chr1:5333172-5333191 MsG0180000388.01.T01:intron 30.0%
!! TTAGTTTGTCTTCAACCAAA+AGG - Chr1:5334069-5334088 None:intergenic 30.0%
!!! ATTTTACTTGCTGGTTCTAA+TGG + Chr1:5331192-5331211 MsG0180000388.01.T01:five_prime_UTR 30.0%
!!! CTGAGTTTTTATTTTTGTCG+GGG + Chr1:5331541-5331560 MsG0180000388.01.T01:intron 30.0%
!!! TTTTGGCTTAATTGCATGTT+TGG - Chr1:5332375-5332394 None:intergenic 30.0%
AAAAAATTAGTGCGCGAGTT+TGG - Chr1:5334098-5334117 None:intergenic 35.0%
AAAAGTCCATGATTGAAGGA+AGG - Chr1:5331004-5331023 None:intergenic 35.0%
AAATGCAGTAGAAAGCAGTT+GGG + Chr1:5334227-5334246 MsG0180000388.01.T01:CDS 35.0%
AATAAATCAGCTCTTACAGG+TGG + Chr1:5333248-5333267 MsG0180000388.01.T01:CDS 35.0%
AGATCAGATGCATACAAGTA+AGG - Chr1:5333865-5333884 None:intergenic 35.0%
ATAAATCAGCTCTTACAGGT+GGG + Chr1:5333249-5333268 MsG0180000388.01.T01:CDS 35.0%
CTTCAAATTGAAGACGTGTT+TGG + Chr1:5333442-5333461 MsG0180000388.01.T01:intron 35.0%
GAAAATGATGACAACGTTGA+AGG + Chr1:5331156-5331175 MsG0180000388.01.T01:five_prime_UTR 35.0%
GAAGAACTACACAGAACAAA+CGG - Chr1:5333985-5334004 None:intergenic 35.0%
GACTTATGTAATGGTGTAAC+AGG + Chr1:5334119-5334138 MsG0180000388.01.T01:intron 35.0%
GCACAATAAACCAAAACAGT+TGG + Chr1:5333712-5333731 MsG0180000388.01.T01:intron 35.0%
GCGTAGATATTGATATCTTC+TGG - Chr1:5334195-5334214 None:intergenic 35.0%
GCGTATCAATTATTGAGGAA+TGG + Chr1:5332859-5332878 MsG0180000388.01.T01:five_prime_UTR 35.0%
GGAAATAAATCAGCTCTTAC+AGG + Chr1:5333245-5333264 MsG0180000388.01.T01:CDS 35.0%
GTCATGCGTATCAATTATTG+AGG + Chr1:5332854-5332873 MsG0180000388.01.T01:five_prime_UTR 35.0%
TATCAATATCTACGCGACAA+CGG + Chr1:5334200-5334219 MsG0180000388.01.T01:CDS 35.0%
TCAAGGCTTAATTGCATGTT+TGG + Chr1:5332010-5332029 MsG0180000388.01.T01:intron 35.0%
TGAGAAAAGTCCATGATTGA+AGG - Chr1:5331008-5331027 None:intergenic 35.0%
TGTTTATGAAGGAGTTCCAA+AGG + Chr1:5332987-5333006 MsG0180000388.01.T01:CDS 35.0%
TTCTTATGACAGGATTACAC+TGG + Chr1:5333182-5333201 MsG0180000388.01.T01:intron 35.0%
TTGTCTCTCTATCAATTCGA+TGG + Chr1:5331863-5331882 MsG0180000388.01.T01:intron 35.0%
! ACGTCTTCAATTTGAAGTGA+CGG - Chr1:5333439-5333458 None:intergenic 35.0%
! GGTGTAACAGGTATTTTATC+TGG + Chr1:5334131-5334150 MsG0180000388.01.T01:intron 35.0%
! GTAACAGGTATTTTATCTGG+AGG + Chr1:5334134-5334153 MsG0180000388.01.T01:intron 35.0%
! TCAACGTTGTCATCATTTTC+AGG - Chr1:5331156-5331175 None:intergenic 35.0%
!! AAAAGCTTAAGCCTTGTTTG+AGG + Chr1:5333619-5333638 MsG0180000388.01.T01:intron 35.0%
!! GGTTTGTTAATTATGACTCC+AGG - Chr1:5332955-5332974 None:intergenic 35.0%
!! TATTTTTGTTCGACACGTGT+GGG - Chr1:5333471-5333490 None:intergenic 35.0%
!! TTGCTGGTTCTAATGGTTAT+TGG + Chr1:5331199-5331218 MsG0180000388.01.T01:five_prime_UTR 35.0%
!! TTTTTGTTGCTTGCTTCATC+AGG + Chr1:5332822-5332841 MsG0180000388.01.T01:intron 35.0%
!!! TGTCATCATTTTCAGGTTCA+TGG - Chr1:5331149-5331168 None:intergenic 35.0%
AATGCAGTAGAAAGCAGTTG+GGG + Chr1:5334228-5334247 MsG0180000388.01.T01:CDS 40.0%
ACGTCTTTAGTGTGAAGTGT+CGG - Chr1:5333044-5333063 None:intergenic 40.0%
AGGAGAATGAATGAGTGAAG+AGG - Chr1:5330984-5331003 None:intergenic 40.0%
AGTGTCATGAGATAAGAGTG+AGG - Chr1:5331702-5331721 None:intergenic 40.0%
CAAATGCAGTAGAAAGCAGT+TGG + Chr1:5334226-5334245 MsG0180000388.01.T01:CDS 40.0%
CATTCTCCTTCCTTCAATCA+TGG + Chr1:5330995-5331014 MsG0180000388.01.T01:five_prime_UTR 40.0%
CTATACAGCATTGCTTGGAA+GGG + Chr1:5334312-5334331 MsG0180000388.01.T01:CDS 40.0%
CTCACTTCTATAGCTGACAT+CGG - Chr1:5332611-5332630 None:intergenic 40.0%
CTGTGTAGTTCTTCCATGTT+TGG + Chr1:5333991-5334010 MsG0180000388.01.T01:intron 40.0%
CTTCCAATGTAGAGAATCCA+AGG + Chr1:5332929-5332948 MsG0180000388.01.T01:CDS 40.0%
CTTGAGATTGAGATGGCAAT+CGG - Chr1:5331113-5331132 None:intergenic 40.0%
GAATCTGGCAGTATCTTTGA+AGG + Chr1:5334162-5334181 MsG0180000388.01.T01:CDS 40.0%
GCGGTGGTATTTAAAAGCTT+TGG - Chr1:5331894-5331913 None:intergenic 40.0%
GTATACTACACTGATCATGG+AGG + Chr1:5333313-5333332 MsG0180000388.01.T01:CDS 40.0%
TCAAGTCGGTCCAAACATAA+GGG - Chr1:5332215-5332234 None:intergenic 40.0%
TCATAAACATCACCACCATC+AGG - Chr1:5332976-5332995 None:intergenic 40.0%
TGGCAATCGGATATAATCTC+CGG - Chr1:5331100-5331119 None:intergenic 40.0%
TTAATTATGACTCCAGGCCT+TGG - Chr1:5332949-5332968 None:intergenic 40.0%
TTCAAGTCGGTCCAAACATA+AGG - Chr1:5332216-5332235 None:intergenic 40.0%
! AGTGGGAAAGTTGTTGATAG+TGG + Chr1:5333272-5333291 MsG0180000388.01.T01:CDS 40.0%
! ATTCCACATGAGCAACGTTT+TGG - Chr1:5332122-5332141 None:intergenic 40.0%
! CTTTTAAATACCACCGCAAG+TGG + Chr1:5331897-5331916 MsG0180000388.01.T01:intron 40.0%
! GATGGTGGTGATGTTTATGA+AGG + Chr1:5332976-5332995 MsG0180000388.01.T01:CDS 40.0%
! GTAAGATGACGGTAAGAAAG+AGG - Chr1:5331043-5331062 None:intergenic 40.0%
!! GTATTTTTGTTCGACACGTG+TGG - Chr1:5333472-5333491 None:intergenic 40.0%
!! TCAAGTTGGTCCCTTATGTT+TGG + Chr1:5332171-5332190 MsG0180000388.01.T01:intron 40.0%
AATGTAGAGAATCCAAGGCC+TGG + Chr1:5332934-5332953 MsG0180000388.01.T01:CDS 45.0%
AATTACTCACCAAGAACCCC+TGG - Chr1:5333340-5333359 None:intergenic 45.0%
AGGCCTTGGATTCTCTACAT+TGG - Chr1:5332935-5332954 None:intergenic 45.0%
ATATAATCTCCGGGGAGTCA+CGG - Chr1:5331090-5331109 None:intergenic 45.0%
ATGACAACGTTGAAGGGACT+AGG + Chr1:5331163-5331182 MsG0180000388.01.T01:five_prime_UTR 45.0%
ATTGCTTGGAAGGGAAGACA+GGG + Chr1:5334321-5334340 MsG0180000388.01.T01:CDS 45.0%
CCTATACAGCATTGCTTGGA+AGG + Chr1:5334311-5334330 MsG0180000388.01.T01:CDS 45.0%
CCTTCCAAGCAATGCTGTAT+AGG - Chr1:5334314-5334333 None:intergenic 45.0%
CGGCAAAGATGGTAAGATGA+CGG - Chr1:5331054-5331073 None:intergenic 45.0%
GACGGTAAGAAAGAGGATAG+TGG - Chr1:5331036-5331055 None:intergenic 45.0%
GAGAATGGACCCTTCAGATT+AGG + Chr1:5331925-5331944 MsG0180000388.01.T01:intron 45.0%
GCAATCGGATATAATCTCCG+GGG - Chr1:5331098-5331117 None:intergenic 45.0%
GCATACAAGTAAGGACCAAC+TGG - Chr1:5333856-5333875 None:intergenic 45.0%
GGCAATCGGATATAATCTCC+GGG - Chr1:5331099-5331118 None:intergenic 45.0%
GTGACCTATACAGCATTGCT+TGG + Chr1:5334307-5334326 MsG0180000388.01.T01:CDS 45.0%
! AAGCAAGTTGAGTACTCTGG+AGG - Chr1:5334285-5334304 None:intergenic 45.0%
! AAGTTGAGTACTCTGGAGGA+GGG - Chr1:5334281-5334300 None:intergenic 45.0%
! CCAGAGTACTCAACTTGCTT+AGG + Chr1:5334285-5334304 MsG0180000388.01.T01:CDS 45.0%
! CCTAAGCAAGTTGAGTACTC+TGG - Chr1:5334288-5334307 None:intergenic 45.0%
! TATCTGGAGGCATGTGAATC+TGG + Chr1:5334147-5334166 MsG0180000388.01.T01:CDS 45.0%
! TTCAGGTTCATGGAAGAACC+TGG - Chr1:5331139-5331158 None:intergenic 45.0%
!!! AGGTGGGCTATTTTACTTGC+TGG + Chr1:5331183-5331202 MsG0180000388.01.T01:five_prime_UTR 45.0%
!!! AAAATTTTAAATTTGATTTT+TGG - Chr1:5332392-5332411 None:intergenic 5.0%
!!! TTTTTTTATAAAAATCAATT+TGG - Chr1:5331967-5331986 None:intergenic 5.0%
AAGGTGAGAGGTAGTTGCAG+TGG - Chr1:5330935-5330954 None:intergenic 50.0%
ACAACGTTGAAGGGACTAGG+TGG + Chr1:5331166-5331185 MsG0180000388.01.T01:five_prime_UTR 50.0%
ACACTGATCATGGAGGTCCA+GGG + Chr1:5333320-5333339 MsG0180000388.01.T01:CDS 50.0%
ACAGGATTACACTGGTGCAG+AGG + Chr1:5333190-5333209 MsG0180000388.01.T01:intron 50.0%
AGGTTCATGGAAGAACCTGG+AGG - Chr1:5331136-5331155 None:intergenic 50.0%
AGTTGGGGAACATATTGCCC+TGG + Chr1:5334243-5334262 MsG0180000388.01.T01:CDS 50.0%
CAACGTTGAAGGGACTAGGT+GGG + Chr1:5331167-5331186 MsG0180000388.01.T01:five_prime_UTR 50.0%
CATCTCAATCTCAAGCCTCC+AGG + Chr1:5331118-5331137 MsG0180000388.01.T01:five_prime_UTR 50.0%
CATTGCTTGGAAGGGAAGAC+AGG + Chr1:5334320-5334339 MsG0180000388.01.T01:CDS 50.0%
CCATTCTCATCGTCCACTTG+CGG - Chr1:5331913-5331932 None:intergenic 50.0%
CTGGAGGCTTGAGATTGAGA+TGG - Chr1:5331120-5331139 None:intergenic 50.0%
GGTCCAAAACGTTGCTCATG+TGG + Chr1:5332116-5332135 MsG0180000388.01.T01:intron 50.0%
GTCGGCAATAGCCTCAAACA+AGG - Chr1:5333633-5333652 None:intergenic 50.0%
GTCTCTTCAGGTATGCCAGT+TGG + Chr1:5333838-5333857 MsG0180000388.01.T01:intron 50.0%
GTTGGGGAACATATTGCCCT+GGG + Chr1:5334244-5334263 MsG0180000388.01.T01:CDS 50.0%
TACACTGATCATGGAGGTCC+AGG + Chr1:5333319-5333338 MsG0180000388.01.T01:CDS 50.0%
TTGGGGAACATATTGCCCTG+GGG + Chr1:5334245-5334264 MsG0180000388.01.T01:CDS 50.0%
! AGTTGAGTACTCTGGAGGAG+GGG - Chr1:5334280-5334299 None:intergenic 50.0%
! CAAGTTGAGTACTCTGGAGG+AGG - Chr1:5334282-5334301 None:intergenic 50.0%
ACCATCTTTGCCGCTGTCTC+CGG + Chr1:5331061-5331080 MsG0180000388.01.T01:five_prime_UTR 55.0%
CACTGATCATGGAGGTCCAG+GGG + Chr1:5333321-5333340 MsG0180000388.01.T01:CDS 55.0%
CAGCTCTTACAGGTGGGAGT+GGG + Chr1:5333255-5333274 MsG0180000388.01.T01:CDS 55.0%
CCGCAAGTGGACGATGAGAA+TGG + Chr1:5331910-5331929 MsG0180000388.01.T01:intron 55.0%
TCAGCTCTTACAGGTGGGAG+TGG + Chr1:5333254-5333273 MsG0180000388.01.T01:CDS 55.0%
TTCTCATCGTCCACTTGCGG+TGG - Chr1:5331910-5331929 None:intergenic 55.0%
! GTTGAGTACTCTGGAGGAGG+GGG - Chr1:5334279-5334298 None:intergenic 55.0%
ACCGGAGACAGCGGCAAAGA+TGG - Chr1:5331065-5331084 None:intergenic 60.0%
CATGGAGGTCCAGGGGTTCT+TGG + Chr1:5333328-5333347 MsG0180000388.01.T01:CDS 60.0%
! AGTACTCTGGAGGAGGGGGA+GGG - Chr1:5334275-5334294 None:intergenic 60.0%
GTCACGGCTACCGGAGACAG+CGG - Chr1:5331074-5331093 None:intergenic 65.0%
! GAGTACTCTGGAGGAGGGGG+AGG - Chr1:5334276-5334295 None:intergenic 65.0%
CTCCGGGGAGTCACGGCTAC+CGG - Chr1:5331083-5331102 None:intergenic 70.0%
CTCCGGTAGCCGTGACTCCC+CGG + Chr1:5331078-5331097 MsG0180000388.01.T01:five_prime_UTR 70.0%
!! GAGGGGGAGGGTACTCCCCA+GGG - Chr1:5334263-5334282 None:intergenic 70.0%
!! GGAGGGGGAGGGTACTCCCC+AGG - Chr1:5334264-5334283 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5330927 5334353 5330927 ID=MsG0180000388.01;Name=MsG0180000388.01
Chr1 mRNA 5330927 5334353 5330927 ID=MsG0180000388.01.T01;Parent=MsG0180000388.01;Name=MsG0180000388.01.T01;_AED=0.50;_eAED=0.50;_QI=379|0.75|0.6|1|1|1|5|0|184
Chr1 exon 5334141 5334353 5334141 ID=MsG0180000388.01.T01:exon:28265;Parent=MsG0180000388.01.T01
Chr1 exon 5333848 5333933 5333848 ID=MsG0180000388.01.T01:exon:28264;Parent=MsG0180000388.01.T01
Chr1 exon 5333194 5333353 5333194 ID=MsG0180000388.01.T01:exon:28263;Parent=MsG0180000388.01.T01
Chr1 exon 5332844 5333008 5332844 ID=MsG0180000388.01.T01:exon:28262;Parent=MsG0180000388.01.T01
Chr1 exon 5330927 5331236 5330927 ID=MsG0180000388.01.T01:exon:28261;Parent=MsG0180000388.01.T01
Chr1 five_prime_UTR 5330927 5331236 5330927 ID=MsG0180000388.01.T01:five_prime_utr;Parent=MsG0180000388.01.T01
Chr1 five_prime_UTR 5332844 5332912 5332844 ID=MsG0180000388.01.T01:five_prime_utr;Parent=MsG0180000388.01.T01
Chr1 CDS 5332913 5333008 5332913 ID=MsG0180000388.01.T01:cds;Parent=MsG0180000388.01.T01
Chr1 CDS 5333194 5333353 5333194 ID=MsG0180000388.01.T01:cds;Parent=MsG0180000388.01.T01
Chr1 CDS 5333848 5333933 5333848 ID=MsG0180000388.01.T01:cds;Parent=MsG0180000388.01.T01
Chr1 CDS 5334141 5334353 5334141 ID=MsG0180000388.01.T01:cds;Parent=MsG0180000388.01.T01
Gene Sequence

>MsG0180000388.01.T01

ATGTATGATGATATTGCTTCCAATGTAGAGAATCCAAGGCCTGGAGTCATAATTAACAAACCTGATGGTGGTGATGTTTATGAAGGAGTTCCAAAGGATTACACTGGTGCAGAGGTACATGCTGACAATTTCTATGCTGCTTTACTTGGAAATAAATCAGCTCTTACAGGTGGGAGTGGGAAAGTTGTTGATAGTGGCCCAAATGATCATATTTTGTATACTACACTGATCATGGAGGTCCAGGGGTTCTTGGTGAGTATGCCAGTTGGTCCTTACTTGTATGCATCTGATCTGAATGAAGTCTTGAAGAAAAAACATGCTTCTGGATCATATAAGAGCCTAGTATTTTATCTGGAGGCATGTGAATCTGGCAGTATCTTTGAAGGTCTTCTTCCAGAAGATATCAATATCTACGCGACAACGGCTTCAAATGCAGTAGAAAGCAGTTGGGGAACATATTGCCCTGGGGAGTACCCTCCCCCTCCTCCAGAGTACTCAACTTGCTTAGGTGACCTATACAGCATTGCTTGGAAGGGAAGACAGGGCTGTAACTAA
Protein sequence

>MsG0180000388.01.T01

MYDDIASNVENPRPGVIINKPDGGDVYEGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHILYTTLIMEVQGFLVSMPVGPYLYASDLNEVLKKKHASGSYKSLVFYLEACESGSIFEGLLPEDINIYATTASNAVESSWGTYCPGEYPPPPPEYSTCLGDLYSIAWKGRQGCN*