AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000389.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000389.01.T01 MTR_1g016780 100.000 47 0 0 3 49 278 324 9.11e-27 100
MsG0180000389.01.T01 MTR_3g104120 71.429 49 12 1 3 49 269 317 5.44e-18 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000389.01.T01 AT4G32940 67.347 49 14 2 3 49 279 327 2.08e-13 63.2
MsG0180000389.01.T01 AT2G25940 59.184 49 18 1 3 49 264 312 9.61e-13 61.2

Find 61 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATCATCTCTTCCTATAT+TGG 0.220532 1:+5334847 MsG0180000389.01.T01:CDS
AAAATAATTTCAAGGGATTA+TGG 0.245835 1:+5336098 MsG0180000389.01.T01:three_prime_UTR
ATGATGATGCTGTATATATT+TGG 0.250928 1:+5336328 MsG0180000389.01.T01:three_prime_UTR
TTTGCACCAGCAATATAAAT+TGG 0.272642 1:+5334583 MsG0180000389.01.T01:CDS
AGAGCTCAATTACTATGATT+TGG 0.274369 1:-5335958 None:intergenic
AACTTACCAATTTATATTGC+TGG 0.287894 1:-5334589 None:intergenic
GACCTTGTTCCCTATCATAT+TGG 0.296528 1:-5336171 None:intergenic
TTGCAGAGAACTCAAGGGTT+TGG 0.350306 1:-5335666 None:intergenic
GGGATTATGGCATTTATAAC+AGG 0.356435 1:+5336111 MsG0180000389.01.T01:three_prime_UTR
CATGATTCTGTAAATACAAT+TGG 0.366402 1:+5335807 MsG0180000389.01.T01:three_prime_UTR
GGTTCTCATGTGATGGAGTA+TGG 0.388016 1:+5334807 MsG0180000389.01.T01:CDS
ACCCTTGAGTTCTCTGCAAA+GGG 0.390198 1:+5335670 MsG0180000389.01.T01:three_prime_UTR
CAATCATCTCTTCCTATATT+GGG 0.390743 1:+5334848 MsG0180000389.01.T01:CDS
CAATGTGTATTGTGTGTAAT+AGG 0.392206 1:+5336193 MsG0180000389.01.T01:three_prime_UTR
TTGTGTGTAATAGGTGTATT+AGG 0.411150 1:+5336202 MsG0180000389.01.T01:three_prime_UTR
AGGACTATCAATGGTTACTA+TGG 0.412584 1:+5334786 MsG0180000389.01.T01:CDS
TCAAGGGTTTGGAGGAATAC+TGG 0.433982 1:-5335655 None:intergenic
TGTGGATCTCTATCTCAGTA+TGG 0.435172 1:+5335547 MsG0180000389.01.T01:CDS
AATACAATTGGAACACTACT+AGG 0.446735 1:+5335819 MsG0180000389.01.T01:three_prime_UTR
TGAGGCCTCGGCCATTTGCT+CGG 0.452146 1:-5335619 None:intergenic
ACAAGCTTGTGCTGAGGCCT+CGG 0.460376 1:-5335631 None:intergenic
TTGGAACACTACTAGGACAT+TGG 0.463310 1:+5335826 MsG0180000389.01.T01:three_prime_UTR
CTACTAGGACATTGGGAGTA+GGG 0.464468 1:+5335834 MsG0180000389.01.T01:three_prime_UTR
CATATTGGACATTCCACAAC+AGG 0.464539 1:-5336156 None:intergenic
GGCGGATTTGTACCCAATAT+AGG 0.464747 1:-5334860 None:intergenic
CAGAGAACTCAAGGGTTTGG+AGG 0.469390 1:-5335663 None:intergenic
AATTCTGTTGCAAAGTGTCT+AGG 0.469631 1:-5336245 None:intergenic
GTAATAGGTGTATTAGGTAT+TGG 0.474726 1:+5336208 MsG0180000389.01.T01:three_prime_UTR
TGGAACACTACTAGGACATT+GGG 0.475553 1:+5335827 MsG0180000389.01.T01:three_prime_UTR
TGTGGAATGTCCAATATGAT+AGG 0.476129 1:+5336161 MsG0180000389.01.T01:three_prime_UTR
TGTATTAGGTATTGGCAGAC+AGG 0.482240 1:+5336216 MsG0180000389.01.T01:three_prime_UTR
TTTGCGAACATCTGCAATGC+AGG 0.487663 1:+5335588 MsG0180000389.01.T01:three_prime_UTR
TGATCTCTACTTGATATTGT+GGG 0.489618 1:+5336004 MsG0180000389.01.T01:three_prime_UTR
ACCCCTTTGCAGAGAACTCA+AGG 0.492877 1:-5335672 None:intergenic
ATGATCTCTACTTGATATTG+TGG 0.495704 1:+5336003 MsG0180000389.01.T01:three_prime_UTR
AGGAATACAAACCGAGCAAA+TGG 0.496796 1:+5335608 MsG0180000389.01.T01:three_prime_UTR
GTGATGGAGTATGGTGATGT+AGG 0.504095 1:+5334816 MsG0180000389.01.T01:CDS
AACCCTTGAGTTCTCTGCAA+AGG 0.521469 1:+5335669 MsG0180000389.01.T01:three_prime_UTR
ACATACTTGTTTGTGACAAG+AGG 0.524150 1:-5336029 None:intergenic
AGACCACATTCTGCCTGTTG+TGG 0.548518 1:+5336143 MsG0180000389.01.T01:three_prime_UTR
ATTCTGTTGCAAAGTGTCTA+GGG 0.556510 1:-5336244 None:intergenic
ACTATGATTTGGATGAACAA+AGG 0.558623 1:-5335947 None:intergenic
GTTAAGGATAGGACTATCAA+TGG 0.561264 1:+5334777 MsG0180000389.01.T01:CDS
CAGTATGGTATGAACATATG+AGG 0.564988 1:+5335562 MsG0180000389.01.T01:CDS
ATTATGGCATTTATAACAGG+AGG 0.570035 1:+5336114 MsG0180000389.01.T01:three_prime_UTR
ATGTGGTGCAGGTTAAGGAT+AGG 0.583690 1:+5334766 MsG0180000389.01.T01:intron
ACAAACCGAGCAAATGGCCG+AGG 0.586423 1:+5335614 MsG0180000389.01.T01:three_prime_UTR
TTACTATGGTTCTCATGTGA+TGG 0.587089 1:+5334800 MsG0180000389.01.T01:CDS
ACTACTAGGACATTGGGAGT+AGG 0.592214 1:+5335833 MsG0180000389.01.T01:three_prime_UTR
TGATGGAGTATGGTGATGTA+GGG 0.594785 1:+5334817 MsG0180000389.01.T01:CDS
TGCATTGCAGATGTTCGCAA+AGG 0.599606 1:-5335586 None:intergenic
CCCCTTTGCAGAGAACTCAA+GGG 0.600306 1:-5335671 None:intergenic
ACAAAGGCTTTATATGTCAC+CGG 0.615717 1:-5335931 None:intergenic
ATACTAAAATAATTGTACAA+AGG 0.616746 1:-5336274 None:intergenic
GTCCAATATGATAGGGAACA+AGG 0.619802 1:+5336169 MsG0180000389.01.T01:three_prime_UTR
GATCTCTACTTGATATTGTG+GGG 0.621074 1:+5336005 MsG0180000389.01.T01:three_prime_UTR
CCCTTGAGTTCTCTGCAAAG+GGG 0.622379 1:+5335671 MsG0180000389.01.T01:three_prime_UTR
GTGGAATGTCCAATATGATA+GGG 0.649089 1:+5336162 MsG0180000389.01.T01:three_prime_UTR
ATTCCACAACAGGCAGAATG+TGG 0.650770 1:-5336146 None:intergenic
ACTGGTACAAGCTTGTGCTG+AGG 0.694000 1:-5335637 None:intergenic
TTATGGCATTTATAACAGGA+GGG 0.694964 1:+5336115 MsG0180000389.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTAAAATAATTGTACAA+AGG - Chr1:5336277-5336296 None:intergenic 15.0%
!! GACTTTACATTAAAAAAATA+TGG + Chr1:5335460-5335479 MsG0180000389.01.T01:intron 15.0%
!! AAAATAATTTCAAGGGATTA+TGG + Chr1:5336098-5336117 MsG0180000389.01.T01:three_prime_UTR 20.0%
!! TGCAAGAAAAATAATTTCAA+GGG + Chr1:5336091-5336110 MsG0180000389.01.T01:three_prime_UTR 20.0%
!! TTGCAAGAAAAATAATTTCA+AGG + Chr1:5336090-5336109 MsG0180000389.01.T01:three_prime_UTR 20.0%
!!! AATGCATAATTCTTTTACTA+TGG + Chr1:5335876-5335895 MsG0180000389.01.T01:intron 20.0%
!!! AATTATGCATTTCTAAGAAA+TGG - Chr1:5335867-5335886 None:intergenic 20.0%
!!! ATTAGTTTAATTGAAATGCA+CGG - Chr1:5335034-5335053 None:intergenic 20.0%
!!! CAGTTTGTTTTTTATTGTAT+AGG + Chr1:5334717-5334736 MsG0180000389.01.T01:intron 20.0%
!!! GTTTTTTATTGTATAGGATT+TGG + Chr1:5334723-5334742 MsG0180000389.01.T01:intron 20.0%
!!! TAATTCTTTTACTATGGTTT+TGG + Chr1:5335882-5335901 MsG0180000389.01.T01:intron 20.0%
!!! TTTTTAATCATTCAGTGATA+GGG + Chr1:5336059-5336078 MsG0180000389.01.T01:three_prime_UTR 20.0%
! AACTTACCAATTTATATTGC+TGG - Chr1:5334592-5334611 None:intergenic 25.0%
! AATATGTGATTAAGCATTCA+AGG - Chr1:5334690-5334709 None:intergenic 25.0%
! CATGATTCTGTAAATACAAT+TGG + Chr1:5335807-5335826 MsG0180000389.01.T01:three_prime_UTR 25.0%
! TGAACAAAAAAAAGCAGATA+AGG - Chr1:5335505-5335524 None:intergenic 25.0%
!! ATGATGATGCTGTATATATT+TGG + Chr1:5336328-5336347 MsG0180000389.01.T01:three_prime_UTR 25.0%
!! GTTTTTAATCATTCAGTGAT+AGG + Chr1:5336058-5336077 MsG0180000389.01.T01:three_prime_UTR 25.0%
!! TCTCAATTTCTTTTGATAGA+TGG + Chr1:5335978-5335997 MsG0180000389.01.T01:three_prime_UTR 25.0%
!! TTTTACAAAGTGCACATTTT+AGG - Chr1:5335712-5335731 None:intergenic 25.0%
!!! TAACCAGTATTGAGTATTTT+TGG + Chr1:5335100-5335119 MsG0180000389.01.T01:intron 25.0%
!!! TTATCTGCTTTTTTTTGTTC+AGG + Chr1:5335504-5335523 MsG0180000389.01.T01:intron 25.0%
AAACATCCAAATGCAATTGA+CGG + Chr1:5335056-5335075 MsG0180000389.01.T01:intron 30.0%
AATACAATTGGAACACTACT+AGG + Chr1:5335819-5335838 MsG0180000389.01.T01:three_prime_UTR 30.0%
ACAATCATCTCTTCCTATAT+TGG + Chr1:5334847-5334866 MsG0180000389.01.T01:CDS 30.0%
ACACCAAAAATACTCAATAC+TGG - Chr1:5335106-5335125 None:intergenic 30.0%
AGAGCTCAATTACTATGATT+TGG - Chr1:5335961-5335980 None:intergenic 30.0%
ATCGTGAGCAACAATTTATA+AGG - Chr1:5334620-5334639 None:intergenic 30.0%
ATGATCTCTACTTGATATTG+TGG + Chr1:5336003-5336022 MsG0180000389.01.T01:three_prime_UTR 30.0%
ATTATGGCATTTATAACAGG+AGG + Chr1:5336114-5336133 MsG0180000389.01.T01:three_prime_UTR 30.0%
CAAAACTAATATTGTCATGG+CGG - Chr1:5334881-5334900 None:intergenic 30.0%
CAATCATCTCTTCCTATATT+GGG + Chr1:5334848-5334867 MsG0180000389.01.T01:CDS 30.0%
CAATGTGTATTGTGTGTAAT+AGG + Chr1:5336193-5336212 MsG0180000389.01.T01:three_prime_UTR 30.0%
CCACAAAACTAATATTGTCA+TGG - Chr1:5334884-5334903 None:intergenic 30.0%
GTAATAGGTGTATTAGGTAT+TGG + Chr1:5336208-5336227 MsG0180000389.01.T01:three_prime_UTR 30.0%
TGATCTCTACTTGATATTGT+GGG + Chr1:5336004-5336023 MsG0180000389.01.T01:three_prime_UTR 30.0%
TTATGGCATTTATAACAGGA+GGG + Chr1:5336115-5336134 MsG0180000389.01.T01:three_prime_UTR 30.0%
TTGTGTGTAATAGGTGTATT+AGG + Chr1:5336202-5336221 MsG0180000389.01.T01:three_prime_UTR 30.0%
TTTGCACCAGCAATATAAAT+TGG + Chr1:5334583-5334602 MsG0180000389.01.T01:CDS 30.0%
! AAATACTCAATACTGGTTAC+TGG - Chr1:5335099-5335118 None:intergenic 30.0%
! ACTATGATTTGGATGAACAA+AGG - Chr1:5335950-5335969 None:intergenic 30.0%
! ATCTCATTCATTTCTGGATA+AGG + Chr1:5334961-5334980 MsG0180000389.01.T01:intron 30.0%
! TGAAGCACAAAAAGAAGTTT+TGG + Chr1:5335170-5335189 MsG0180000389.01.T01:intron 30.0%
!! TGATGTCTTATTTGCTAATG+TGG + Chr1:5334749-5334768 MsG0180000389.01.T01:intron 30.0%
!!! CCATGACAATATTAGTTTTG+TGG + Chr1:5334881-5334900 MsG0180000389.01.T01:intron 30.0%
!!! TGAGTATTTTTGGTGTTCAT+TGG + Chr1:5335110-5335129 MsG0180000389.01.T01:intron 30.0%
AATCAAGAGAAGTAGTACCA+TGG - Chr1:5335337-5335356 None:intergenic 35.0%
ACATACTTGTTTGTGACAAG+AGG - Chr1:5336032-5336051 None:intergenic 35.0%
AGGACTATCAATGGTTACTA+TGG + Chr1:5334786-5334805 MsG0180000389.01.T01:CDS 35.0%
AGTCATGCAAATTCATCACA+AGG - Chr1:5335444-5335463 None:intergenic 35.0%
ATGCTGATCTCATTCATTTC+TGG + Chr1:5334955-5334974 MsG0180000389.01.T01:intron 35.0%
CAGTATGGTATGAACATATG+AGG + Chr1:5335562-5335581 MsG0180000389.01.T01:CDS 35.0%
CTGATTGGAAAGCTCTTATT+TGG + Chr1:5335234-5335253 MsG0180000389.01.T01:intron 35.0%
GATCTCTACTTGATATTGTG+GGG + Chr1:5336005-5336024 MsG0180000389.01.T01:three_prime_UTR 35.0%
GGGATTATGGCATTTATAAC+AGG + Chr1:5336111-5336130 MsG0180000389.01.T01:three_prime_UTR 35.0%
GTGGAATGTCCAATATGATA+GGG + Chr1:5336162-5336181 MsG0180000389.01.T01:three_prime_UTR 35.0%
GTTAAGGATAGGACTATCAA+TGG + Chr1:5334777-5334796 MsG0180000389.01.T01:CDS 35.0%
TCAACAGCAGTTTAACCAAA+GGG + Chr1:5334932-5334951 MsG0180000389.01.T01:intron 35.0%
TGTGGAATGTCCAATATGAT+AGG + Chr1:5336161-5336180 MsG0180000389.01.T01:three_prime_UTR 35.0%
TTACTATGGTTCTCATGTGA+TGG + Chr1:5334800-5334819 MsG0180000389.01.T01:CDS 35.0%
TTCAACAGCAGTTTAACCAA+AGG + Chr1:5334931-5334950 MsG0180000389.01.T01:intron 35.0%
TTCAGCTTCATCTATCTTTC+TGG + Chr1:5335355-5335374 MsG0180000389.01.T01:intron 35.0%
TTGAAGGTGATCTCATTTCA+AGG - Chr1:5334916-5334935 None:intergenic 35.0%
TTTGGTTAAACTGCTGTTGA+AGG - Chr1:5334932-5334951 None:intergenic 35.0%
! AATTCTGTTGCAAAGTGTCT+AGG - Chr1:5336248-5336267 None:intergenic 35.0%
! ACAAAGGCTTTATATGTCAC+CGG - Chr1:5335934-5335953 None:intergenic 35.0%
! AGCTCTTATTTGGCATTGAA+AGG + Chr1:5335244-5335263 MsG0180000389.01.T01:intron 35.0%
! ATTCTGTTGCAAAGTGTCTA+GGG - Chr1:5336247-5336266 None:intergenic 35.0%
!! TTTTGTGCTTCATTTTTCCG+CGG - Chr1:5335162-5335181 None:intergenic 35.0%
!!! AAGACTTTTGAGACACATTG+TGG + Chr1:5335529-5335548 MsG0180000389.01.T01:CDS 35.0%
!!! TATGGTTTTGGATAGAACTG+AGG + Chr1:5335894-5335913 MsG0180000389.01.T01:intron 35.0%
AGGAATACAAACCGAGCAAA+TGG + Chr1:5335608-5335627 MsG0180000389.01.T01:three_prime_UTR 40.0%
CATATTGGACATTCCACAAC+AGG - Chr1:5336159-5336178 None:intergenic 40.0%
CATGGGAGCAATGATAAAAC+TGG + Chr1:5335418-5335437 MsG0180000389.01.T01:intron 40.0%
GACAACAGTGTGAAACTGAT+TGG + Chr1:5335219-5335238 MsG0180000389.01.T01:intron 40.0%
GACCTTGTTCCCTATCATAT+TGG - Chr1:5336174-5336193 None:intergenic 40.0%
GTCCAATATGATAGGGAACA+AGG + Chr1:5336169-5336188 MsG0180000389.01.T01:three_prime_UTR 40.0%
TGGAACACTACTAGGACATT+GGG + Chr1:5335827-5335846 MsG0180000389.01.T01:three_prime_UTR 40.0%
TGGTTACTGGATCTTGTTCT+CGG - Chr1:5335086-5335105 None:intergenic 40.0%
TGTGGATCTCTATCTCAGTA+TGG + Chr1:5335547-5335566 MsG0180000389.01.T01:CDS 40.0%
TTGGAACACTACTAGGACAT+TGG + Chr1:5335826-5335845 MsG0180000389.01.T01:three_prime_UTR 40.0%
! CTTATTTGCTAATGTGGTGC+AGG + Chr1:5334755-5334774 MsG0180000389.01.T01:intron 40.0%
! TGATGGAGTATGGTGATGTA+GGG + Chr1:5334817-5334836 MsG0180000389.01.T01:CDS 40.0%
! TGCTTTTATGCCTAAGCCAT+GGG + Chr1:5335401-5335420 MsG0180000389.01.T01:intron 40.0%
!! CAGTTTTATCATTGCTCCCA+TGG - Chr1:5335420-5335439 None:intergenic 40.0%
!! GAAGTAGTACCATGGTTTTG+AGG - Chr1:5335329-5335348 None:intergenic 40.0%
!! TGTATTAGGTATTGGCAGAC+AGG + Chr1:5336216-5336235 MsG0180000389.01.T01:three_prime_UTR 40.0%
!! TTGCTTTTATGCCTAAGCCA+TGG + Chr1:5335400-5335419 MsG0180000389.01.T01:intron 40.0%
!! TTTTGGAAGCAATGTCTCAC+AGG + Chr1:5335187-5335206 MsG0180000389.01.T01:intron 40.0%
AACCCTTGAGTTCTCTGCAA+AGG + Chr1:5335669-5335688 MsG0180000389.01.T01:three_prime_UTR 45.0%
ACCCTTGAGTTCTCTGCAAA+GGG + Chr1:5335670-5335689 MsG0180000389.01.T01:three_prime_UTR 45.0%
ACTACTAGGACATTGGGAGT+AGG + Chr1:5335833-5335852 MsG0180000389.01.T01:three_prime_UTR 45.0%
ACTGGATTGCCTCAAAACCA+TGG + Chr1:5335317-5335336 MsG0180000389.01.T01:intron 45.0%
ATGTGGTGCAGGTTAAGGAT+AGG + Chr1:5334766-5334785 MsG0180000389.01.T01:intron 45.0%
ATTCCACAACAGGCAGAATG+TGG - Chr1:5336149-5336168 None:intergenic 45.0%
CTACTAGGACATTGGGAGTA+GGG + Chr1:5335834-5335853 MsG0180000389.01.T01:three_prime_UTR 45.0%
GAATGAGATCAGCATCCCTT+TGG - Chr1:5334950-5334969 None:intergenic 45.0%
GGTTACTGGATCTTGTTCTC+GGG - Chr1:5335085-5335104 None:intergenic 45.0%
TATCATTGCTCCCATGGCTT+AGG - Chr1:5335414-5335433 None:intergenic 45.0%
TCAAGGGTTTGGAGGAATAC+TGG - Chr1:5335658-5335677 None:intergenic 45.0%
TGCATTGCAGATGTTCGCAA+AGG - Chr1:5335589-5335608 None:intergenic 45.0%
TGCTAATGTGGTGCAGGTTA+AGG + Chr1:5334761-5334780 MsG0180000389.01.T01:intron 45.0%
TTGCAGAGAACTCAAGGGTT+TGG - Chr1:5335669-5335688 None:intergenic 45.0%
TTTGCGAACATCTGCAATGC+AGG + Chr1:5335588-5335607 MsG0180000389.01.T01:three_prime_UTR 45.0%
! GGCGGATTTGTACCCAATAT+AGG - Chr1:5334863-5334882 None:intergenic 45.0%
! GGTTCTCATGTGATGGAGTA+TGG + Chr1:5334807-5334826 MsG0180000389.01.T01:CDS 45.0%
! GTGATGGAGTATGGTGATGT+AGG + Chr1:5334816-5334835 MsG0180000389.01.T01:CDS 45.0%
!! TGAGGTACGCGTTTTAGTAC+CGG + Chr1:5335912-5335931 MsG0180000389.01.T01:intron 45.0%
ACCCCTTTGCAGAGAACTCA+AGG - Chr1:5335675-5335694 None:intergenic 50.0%
ACTGGTACAAGCTTGTGCTG+AGG - Chr1:5335640-5335659 None:intergenic 50.0%
AGACCACATTCTGCCTGTTG+TGG + Chr1:5336143-5336162 MsG0180000389.01.T01:three_prime_UTR 50.0%
CAGAGAACTCAAGGGTTTGG+AGG - Chr1:5335666-5335685 None:intergenic 50.0%
CCCCTTTGCAGAGAACTCAA+GGG - Chr1:5335674-5335693 None:intergenic 50.0%
CCCTTGAGTTCTCTGCAAAG+GGG + Chr1:5335671-5335690 MsG0180000389.01.T01:three_prime_UTR 50.0%
CTCGACAATGTTAGACCTGC+TGG + Chr1:5335277-5335296 MsG0180000389.01.T01:intron 50.0%
GATCACCACTCGTTGACAAC+TGG + Chr1:5335299-5335318 MsG0180000389.01.T01:intron 50.0%
GCAATCCAGTTGTCAACGAG+TGG - Chr1:5335307-5335326 None:intergenic 50.0%
GGGCATCCGTCAATTGCATT+TGG - Chr1:5335065-5335084 None:intergenic 50.0%
! ATTTTTCCGCGGAGAACCTC+AGG - Chr1:5335151-5335170 None:intergenic 50.0%
ACAAACCGAGCAAATGGCCG+AGG + Chr1:5335614-5335633 MsG0180000389.01.T01:three_prime_UTR 55.0%
ACAAGCTTGTGCTGAGGCCT+CGG - Chr1:5335634-5335653 None:intergenic 55.0%
GGAGAACCTCAGGTGCTTTG+CGG - Chr1:5335141-5335160 None:intergenic 55.0%
TCAACGAGTGGTGATCCAGC+AGG - Chr1:5335295-5335314 None:intergenic 55.0%
! AAAGCACCTGAGGTTCTCCG+CGG + Chr1:5335142-5335161 MsG0180000389.01.T01:intron 55.0%
TGAGGCCTCGGCCATTTGCT+CGG - Chr1:5335622-5335641 None:intergenic 60.0%
! GCAGTTCCGCAAAGCACCTG+AGG + Chr1:5335132-5335151 MsG0180000389.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5334548 5336373 5334548 ID=MsG0180000389.01;Name=MsG0180000389.01
Chr1 mRNA 5334548 5336373 5334548 ID=MsG0180000389.01.T01;Parent=MsG0180000389.01;Name=MsG0180000389.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0.66|0.5|1|0.33|0|4|725|68
Chr1 exon 5335922 5336373 5335922 ID=MsG0180000389.01.T01:exon:28260;Parent=MsG0180000389.01.T01
Chr1 exon 5335526 5335855 5335526 ID=MsG0180000389.01.T01:exon:28259;Parent=MsG0180000389.01.T01
Chr1 exon 5334777 5334869 5334777 ID=MsG0180000389.01.T01:exon:28258;Parent=MsG0180000389.01.T01
Chr1 exon 5334548 5334604 5334548 ID=MsG0180000389.01.T01:exon:28257;Parent=MsG0180000389.01.T01
Chr1 CDS 5334548 5334604 5334548 ID=MsG0180000389.01.T01:cds;Parent=MsG0180000389.01.T01
Chr1 CDS 5334777 5334869 5334777 ID=MsG0180000389.01.T01:cds;Parent=MsG0180000389.01.T01
Chr1 CDS 5335526 5335582 5335526 ID=MsG0180000389.01.T01:cds;Parent=MsG0180000389.01.T01
Chr1 three_prime_UTR 5335583 5335855 5335583 ID=MsG0180000389.01.T01:three_prime_utr;Parent=MsG0180000389.01.T01
Chr1 three_prime_UTR 5335922 5336373 5335922 ID=MsG0180000389.01.T01:three_prime_utr;Parent=MsG0180000389.01.T01
Gene Sequence

>MsG0180000389.01.T01

ATGAGCAGTGACATACACAATTTACGAACTGAAAGTTTGCACCAGCAATATAAATTGGTTAAGGATAGGACTATCAATGGTTACTATGGTTCTCATGTGATGGAGTATGGTGATGTAGGGCTTAGCAACAATCATCTCTTCCTATATTGGGTGAAGACTTTTGAGACACATTGTGGATCTCTATCTCAGTATGGTATGAACATATGA
Protein sequence

>MsG0180000389.01.T01

MSSDIHNLRTESLHQQYKLVKDRTINGYYGSHVMEYGDVGLSNNHLFLYWVKTFETHCGSLSQYGMNI*