Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000405.01.T01 | XP_003589025.2 | 99.471 | 189 | 1 | 0 | 1 | 189 | 18 | 206 | 4.27E-136 | 389 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000405.01.T01 | O80992 | 69.312 | 189 | 54 | 3 | 1 | 187 | 1 | 187 | 3.93E-95 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000405.01.T01 | G7IB92 | 99.471 | 189 | 1 | 0 | 1 | 189 | 18 | 206 | 2.04e-136 | 389 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480018569.01 | MsG0180000405.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000405.01.T01 | MTR_1g016480 | 99.471 | 189 | 1 | 0 | 1 | 189 | 18 | 206 | 5.17e-140 | 389 |
| MsG0180000405.01.T01 | MTR_5g030500 | 55.429 | 175 | 72 | 3 | 9 | 178 | 31 | 204 | 1.86e-66 | 203 |
| MsG0180000405.01.T01 | MTR_3g071740 | 58.333 | 156 | 57 | 3 | 26 | 179 | 72 | 221 | 1.60e-59 | 186 |
| MsG0180000405.01.T01 | MTR_4g014460 | 52.201 | 159 | 74 | 2 | 22 | 179 | 49 | 206 | 1.66e-53 | 170 |
| MsG0180000405.01.T01 | MTR_7g070050 | 52.601 | 173 | 77 | 3 | 22 | 189 | 13 | 185 | 3.79e-53 | 168 |
| MsG0180000405.01.T01 | MTR_8g027805 | 46.512 | 172 | 90 | 2 | 9 | 179 | 4 | 174 | 7.96e-53 | 167 |
| MsG0180000405.01.T01 | MTR_5g083270 | 53.165 | 158 | 67 | 3 | 25 | 179 | 49 | 202 | 9.17e-53 | 167 |
| MsG0180000405.01.T01 | MTR_1g028380 | 49.367 | 158 | 79 | 1 | 22 | 179 | 19 | 175 | 1.10e-52 | 167 |
| MsG0180000405.01.T01 | MTR_3g090980 | 46.471 | 170 | 82 | 3 | 11 | 179 | 16 | 177 | 1.33e-52 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000405.01.T01 | AT2G26040 | 69.312 | 189 | 54 | 3 | 1 | 187 | 1 | 187 | 4.01e-96 | 277 |
| MsG0180000405.01.T01 | AT1G73000 | 59.783 | 184 | 64 | 2 | 9 | 182 | 24 | 207 | 2.85e-78 | 233 |
| MsG0180000405.01.T01 | AT4G17870 | 54.070 | 172 | 76 | 2 | 10 | 179 | 5 | 175 | 2.36e-64 | 197 |
| MsG0180000405.01.T01 | AT2G40330 | 54.777 | 157 | 66 | 4 | 26 | 179 | 54 | 208 | 9.09e-55 | 173 |
| MsG0180000405.01.T01 | AT5G46790 | 49.711 | 173 | 83 | 2 | 10 | 178 | 32 | 204 | 4.15e-54 | 172 |
| MsG0180000405.01.T01 | AT5G46790 | 49.711 | 173 | 83 | 2 | 10 | 178 | 32 | 204 | 4.15e-54 | 172 |
| MsG0180000405.01.T01 | AT5G05440 | 55.414 | 157 | 64 | 4 | 22 | 176 | 45 | 197 | 1.29e-53 | 170 |
| MsG0180000405.01.T01 | AT5G53160 | 45.763 | 177 | 91 | 3 | 4 | 179 | 3 | 175 | 2.87e-52 | 166 |
| MsG0180000405.01.T01 | AT4G01026 | 47.170 | 159 | 81 | 2 | 22 | 179 | 23 | 179 | 7.81e-52 | 166 |
| MsG0180000405.01.T01 | AT1G01360 | 45.509 | 167 | 88 | 2 | 15 | 179 | 12 | 177 | 4.67e-51 | 163 |
| MsG0180000405.01.T01 | AT2G38310 | 56.329 | 158 | 63 | 3 | 24 | 179 | 41 | 194 | 5.98e-50 | 160 |
| MsG0180000405.01.T01 | AT4G27920 | 47.799 | 159 | 79 | 3 | 22 | 178 | 14 | 170 | 1.87e-46 | 151 |
| MsG0180000405.01.T01 | AT5G45860 | 52.258 | 155 | 64 | 3 | 23 | 176 | 5 | 150 | 6.70e-45 | 146 |
| MsG0180000405.01.T01 | AT5G45870 | 53.147 | 143 | 63 | 3 | 35 | 176 | 10 | 149 | 1.89e-41 | 137 |
| MsG0180000405.01.T01 | AT4G18620 | 47.651 | 149 | 70 | 2 | 35 | 176 | 9 | 156 | 6.56e-35 | 121 |
| MsG0180000405.01.T01 | AT5G53160 | 50.000 | 118 | 57 | 2 | 4 | 121 | 3 | 118 | 1.83e-34 | 119 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTGAAAGATTAGAGATTT+TGG | 0.170116 | 1:-5585585 | MsG0180000405.01.T01:CDS |
| AGAGAAGTTACCGTTGTTTC+GGG | 0.231870 | 1:-5585626 | MsG0180000405.01.T01:CDS |
| TACCGTTGTTTCGGGTTTAC+CGG | 0.281112 | 1:-5585618 | MsG0180000405.01.T01:CDS |
| TAAGGTTTATACTATTGTTT+TGG | 0.288773 | 1:-5585459 | MsG0180000405.01.T01:CDS |
| TAGAGAAGTTACCGTTGTTT+CGG | 0.323518 | 1:-5585627 | MsG0180000405.01.T01:CDS |
| TTCTCAAAGCTTCTAACATA+TGG | 0.399534 | 1:+5585716 | None:intergenic |
| GATTGAAGTGCATGTGTTTG+AGG | 0.427491 | 1:+5585778 | None:intergenic |
| AAGCTTAATCTTCAGAAACT+TGG | 0.437353 | 1:-5585365 | MsG0180000405.01.T01:CDS |
| AATGAGTTTGTCAACAATGA+AGG | 0.452521 | 1:-5585482 | MsG0180000405.01.T01:CDS |
| GCTTCGGCGCATAATATTCA+AGG | 0.486800 | 1:-5585866 | MsG0180000405.01.T01:CDS |
| AAACTATCGTTCGGTTACTT+CGG | 0.496046 | 1:-5585507 | MsG0180000405.01.T01:CDS |
| TATGGCCACACTATGCTTGA+TGG | 0.499255 | 1:+5585734 | None:intergenic |
| GTTTGTCAACAATGAAGGTA+AGG | 0.501378 | 1:-5585477 | MsG0180000405.01.T01:CDS |
| TTGAGGTTGGTTCTAATATG+TGG | 0.501993 | 1:+5585795 | None:intergenic |
| AACAGTGTCAACAAACATCT+TGG | 0.503428 | 1:+5585391 | None:intergenic |
| TCGTCTTCAAAACTATCGTT+CGG | 0.506349 | 1:-5585516 | MsG0180000405.01.T01:CDS |
| GAAGTGCATGTGTTTGAGGT+TGG | 0.508894 | 1:+5585782 | None:intergenic |
| AAACACCCACAAAATGGCTT+CGG | 0.524476 | 1:-5585882 | None:intergenic |
| ATCAAAGGTTGCAACATGAA+AGG | 0.524876 | 1:-5585671 | MsG0180000405.01.T01:CDS |
| TATATATTATTGTCCATGCA+TGG | 0.529703 | 1:+5585316 | None:intergenic |
| AGTTCATGTTCTTCCATGCA+TGG | 0.533444 | 1:-5585329 | MsG0180000405.01.T01:CDS |
| CTTTCAGTACTTGTCGAAGC+CGG | 0.537971 | 1:+5585599 | None:intergenic |
| AAATACAAACATTTCATCAA+AGG | 0.541877 | 1:-5585686 | MsG0180000405.01.T01:CDS |
| GGTTGCAACATGAAAGGTGA+TGG | 0.558210 | 1:-5585665 | MsG0180000405.01.T01:CDS |
| TATATTGTTGATATTCCACA+TGG | 0.600252 | 1:-5585431 | MsG0180000405.01.T01:CDS |
| TCTTCTTCAGTGTTCCCATG+TGG | 0.603865 | 1:+5585416 | None:intergenic |
| ATGAAAGGTGATGGAAACGT+TGG | 0.615437 | 1:-5585656 | MsG0180000405.01.T01:CDS |
| AGCCGGTAAACCCGAAACAA+CGG | 0.615768 | 1:+5585616 | None:intergenic |
| AAGCACCATCAAGCATAGTG+TGG | 0.678529 | 1:-5585739 | MsG0180000405.01.T01:CDS |
| ATATTGTTGATATTCCACAT+GGG | 0.707262 | 1:-5585430 | MsG0180000405.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACAAACATTTCATCAA+AGG | - | Chr1:5585505-5585524 | MsG0180000405.01.T01:CDS | 20.0% |
| !!! | ATGAAATGTTTGTATTTTTG+TGG | + | Chr1:5585502-5585521 | None:intergenic | 20.0% |
| !!! | ATGTGGTATTTTTTTATGAT+TGG | + | Chr1:5585382-5585401 | None:intergenic | 20.0% |
| !!! | TAAGGTTTATACTATTGTTT+TGG | - | Chr1:5585732-5585751 | MsG0180000405.01.T01:CDS | 20.0% |
| ! | ATATTGTTGATATTCCACAT+GGG | - | Chr1:5585761-5585780 | MsG0180000405.01.T01:CDS | 25.0% |
| ! | TATATTGTTGATATTCCACA+TGG | - | Chr1:5585760-5585779 | MsG0180000405.01.T01:CDS | 25.0% |
| !! | TACTGAAAGATTAGAGATTT+TGG | - | Chr1:5585606-5585625 | MsG0180000405.01.T01:CDS | 25.0% |
| !!! | GTAATAAGTTTTAGAGTTGT+TGG | - | Chr1:5585643-5585662 | MsG0180000405.01.T01:CDS | 25.0% |
| AAGCTTAATCTTCAGAAACT+TGG | - | Chr1:5585826-5585845 | MsG0180000405.01.T01:CDS | 30.0% | |
| AATGAGTTTGTCAACAATGA+AGG | - | Chr1:5585709-5585728 | MsG0180000405.01.T01:CDS | 30.0% | |
| TTCTCAAAGCTTCTAACATA+TGG | + | Chr1:5585478-5585497 | None:intergenic | 30.0% | |
| !!! | ATAAGTTTTAGAGTTGTTGG+TGG | - | Chr1:5585646-5585665 | MsG0180000405.01.T01:CDS | 30.0% |
| AAACTATCGTTCGGTTACTT+CGG | - | Chr1:5585684-5585703 | MsG0180000405.01.T01:CDS | 35.0% | |
| AACAGTGTCAACAAACATCT+TGG | + | Chr1:5585803-5585822 | None:intergenic | 35.0% | |
| ATCAAAGGTTGCAACATGAA+AGG | - | Chr1:5585520-5585539 | MsG0180000405.01.T01:CDS | 35.0% | |
| GTTTGTCAACAATGAAGGTA+AGG | - | Chr1:5585714-5585733 | MsG0180000405.01.T01:CDS | 35.0% | |
| TAGAGAAGTTACCGTTGTTT+CGG | - | Chr1:5585564-5585583 | MsG0180000405.01.T01:CDS | 35.0% | |
| TCGTCTTCAAAACTATCGTT+CGG | - | Chr1:5585675-5585694 | MsG0180000405.01.T01:CDS | 35.0% | |
| !! | TGATGGTGCTTCTATTTTGT+AGG | + | Chr1:5585443-5585462 | None:intergenic | 35.0% |
| !! | TTGAGGTTGGTTCTAATATG+TGG | + | Chr1:5585399-5585418 | None:intergenic | 35.0% |
| AGTTCATGTTCTTCCATGCA+TGG | - | Chr1:5585862-5585881 | MsG0180000405.01.T01:CDS | 40.0% | |
| GATTGAAGTGCATGTGTTTG+AGG | + | Chr1:5585416-5585435 | None:intergenic | 40.0% | |
| ! | AGAGAAGTTACCGTTGTTTC+GGG | - | Chr1:5585565-5585584 | MsG0180000405.01.T01:CDS | 40.0% |
| !! | ATGAAAGGTGATGGAAACGT+TGG | - | Chr1:5585535-5585554 | MsG0180000405.01.T01:CDS | 40.0% |
| AAGCACCATCAAGCATAGTG+TGG | - | Chr1:5585452-5585471 | MsG0180000405.01.T01:CDS | 45.0% | |
| CTTTCAGTACTTGTCGAAGC+CGG | + | Chr1:5585595-5585614 | None:intergenic | 45.0% | |
| GCTTCGGCGCATAATATTCA+AGG | - | Chr1:5585325-5585344 | MsG0180000405.01.T01:CDS | 45.0% | |
| GGTTGCAACATGAAAGGTGA+TGG | - | Chr1:5585526-5585545 | MsG0180000405.01.T01:CDS | 45.0% | |
| TATGGCCACACTATGCTTGA+TGG | + | Chr1:5585460-5585479 | None:intergenic | 45.0% | |
| TCTTCTTCAGTGTTCCCATG+TGG | + | Chr1:5585778-5585797 | None:intergenic | 45.0% | |
| ! | GAAGTGCATGTGTTTGAGGT+TGG | + | Chr1:5585412-5585431 | None:intergenic | 45.0% |
| !! | TACCGTTGTTTCGGGTTTAC+CGG | - | Chr1:5585573-5585592 | MsG0180000405.01.T01:CDS | 45.0% |
| AGCCGGTAAACCCGAAACAA+CGG | + | Chr1:5585578-5585597 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 5585322 | 5585891 | 5585322 | ID=MsG0180000405.01;Name=MsG0180000405.01 |
| Chr1 | mRNA | 5585322 | 5585891 | 5585322 | ID=MsG0180000405.01.T01;Parent=MsG0180000405.01;Name=MsG0180000405.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|189 |
| Chr1 | exon | 5585322 | 5585891 | 5585322 | ID=MsG0180000405.01.T01:exon:2698;Parent=MsG0180000405.01.T01 |
| Chr1 | CDS | 5585322 | 5585891 | 5585322 | ID=MsG0180000405.01.T01:cds;Parent=MsG0180000405.01.T01 |
| Gene Sequence |
| Protein sequence |
