AlfalfaGEDB | MsG0180000443.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000443.01

KEGG: -
GO: -
PFAM: PF12023

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000443.01.T01	XP_013465885.1	96.639	119	4	1	119	1	119	9.11E-64	199
MsG0180000443.01.T03	XP_013465885.1	100	31	0	1	31	89	119	7.71E-13	67
MsG0180000443.01.T02	XP_013465885.1	97.115	104	3	1	104	16	119	5.07E-66	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000443.01.T01	A0A072VEC4	96.639	119	4	1	119	1	119	4.35e-64	199
MsG0180000443.01.T02	A0A072VEC4	97.115	104	3	1	104	16	119	2.42e-66	204
MsG0180000443.01.T03	A0A072VEC4	100.000	31	0	1	31	89	119	3.68e-13	67.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000443.01	MsG0880044644.01	0.803346	3.643026e-49	1.826287e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000443.01.T01	MTR_1g015810	96.639	119	4	0	1	119	1	119	1.10e-67	199
MsG0180000443.01.T01	MTR_3g104640	67.241	116	30	5	1	116	1	108	1.09e-30	105
MsG0180000443.01.T01	MTR_4g125990	54.264	129	38	9	1	116	1	121	3.03e-27	97.8
MsG0180000443.01.T01	MTR_2g099630	50.000	90	37	3	31	119	26	108	6.90e-22	83.6
MsG0180000443.01.T01	MTR_8g018320	51.899	79	32	3	31	109	26	98	3.18e-16	68.9
MsG0180000443.01.T01	MTR_8g018360	48.235	85	38	3	31	115	26	104	1.61e-15	69.3
MsG0180000443.01.T01	MTR_7g109290	41.667	60	34	1	58	117	32	90	6.49e-11	55.1
MsG0180000443.01.T03	MTR_1g015810	100.000	31	0	0	1	31	89	119	9.34e-17	67.0
MsG0180000443.01.T03	MTR_3g104640	85.714	28	4	0	1	28	81	108	3.63e-11	52.4
MsG0180000443.01.T02	MTR_1g015810	97.115	104	3	0	1	104	16	119	6.14e-70	204
MsG0180000443.01.T02	MTR_3g104640	71.287	101	22	4	1	101	15	108	1.70e-27	97.4
MsG0180000443.01.T02	MTR_4g125990	58.407	113	29	7	2	101	14	121	2.81e-27	97.1
MsG0180000443.01.T02	MTR_2g099630	50.000	90	37	3	16	104	26	108	3.88e-22	83.6
MsG0180000443.01.T02	MTR_8g018320	51.899	79	32	3	16	94	26	98	2.19e-16	68.9
MsG0180000443.01.T02	MTR_8g018360	48.235	85	38	3	16	100	26	104	1.18e-15	68.9
MsG0180000443.01.T02	MTR_7g109290	41.667	60	34	1	43	102	32	90	4.59e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000443.01.T01	AT5G11970	54.622	119	40	7	1	119	1	105	2.87e-27	97.1
MsG0180000443.01.T01	AT3G13910	51.852	81	35	3	30	109	20	97	3.89e-16	68.6
MsG0180000443.01.T01	AT2G19460	43.333	120	48	5	1	110	1	110	8.47e-16	68.2
MsG0180000443.01.T01	AT3G13910	51.852	81	35	3	30	109	20	97	8.82e-16	68.2
MsG0180000443.01.T01	AT2G19460	43.333	120	48	5	1	110	53	162	1.76e-15	68.6
MsG0180000443.01.T01	AT1G72720	43.158	95	48	2	27	119	37	127	1.66e-13	62.8
MsG0180000443.01.T01	AT2G47480	45.161	62	32	1	57	116	47	108	1.33e-12	59.7
MsG0180000443.01.T01	AT3G05725	43.478	69	36	2	50	118	59	124	1.87e-11	57.4
MsG0180000443.01.T01	AT3G62640	52.381	42	20	0	75	116	68	109	1.94e-11	57.0
MsG0180000443.01.T02	AT5G11970	55.340	103	34	5	2	104	15	105	1.93e-23	87.0
MsG0180000443.01.T02	AT3G13910	51.852	81	35	3	15	94	20	97	2.38e-16	68.6
MsG0180000443.01.T02	AT3G13910	51.852	81	35	3	15	94	20	97	4.45e-16	68.6
MsG0180000443.01.T02	AT2G19460	55.000	80	28	3	16	95	39	110	1.57e-14	64.3
MsG0180000443.01.T02	AT2G19460	55.000	80	28	3	16	95	91	162	2.69e-14	65.1
MsG0180000443.01.T02	AT1G72720	42.157	102	53	2	5	104	30	127	9.02e-14	62.8
MsG0180000443.01.T02	AT2G47480	45.161	62	32	1	42	101	47	108	1.01e-12	59.7
MsG0180000443.01.T02	AT3G05725	43.478	69	36	2	35	103	59	124	1.32e-11	57.0
MsG0180000443.01.T02	AT3G62640	52.381	42	20	0	60	101	68	109	1.41e-11	56.6

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTCAAGTCCTTATTATT+AGG	0.268756	1:+6113196	None:intergenic
GAAAAGTGAAAGGTTCATTT+AGG	0.301962	1:-6113054	MsG0180000443.01.T01:CDS
TCAAAGGGGTCCTAATAATA+AGG	0.325607	1:-6113206	MsG0180000443.01.T01:CDS
TAAGACCTAATATCATAATT+AGG	0.344095	1:+6113253	None:intergenic
ATTTCCAAGTCTTGGAGCTT+TGG	0.345743	1:-6113139	MsG0180000443.01.T01:CDS
CTAAGCCTAATTATGATATT+AGG	0.367646	1:-6113258	MsG0180000443.01.T01:CDS
CTGAATTTCAAAGAAAGAAA+AGG	0.382930	1:-6113108	MsG0180000443.01.T01:CDS
GGTCTTCAATTTCCAAGTCT+TGG	0.385601	1:-6113147	MsG0180000443.01.T01:CDS
AGGATTACAGCAAACAAATT+AGG	0.410868	1:-6113330	MsG0180000443.01.T01:CDS
TTCCTATGCACAAACTCAAA+GGG	0.443805	1:-6113221	MsG0180000443.01.T01:CDS
AGCAAACAAATTAGGCCCTA+TGG	0.447501	1:-6113322	MsG0180000443.01.T01:CDS
AGTGGCTAAAGAATAGGTAT+TGG	0.458659	1:-6113021	MsG0180000443.01.T01:CDS
TGAATTTCAAAGAAAGAAAA+GGG	0.462597	1:-6113107	MsG0180000443.01.T01:CDS
GCTTTAAGTGGCTAAAGAAT+AGG	0.471892	1:-6113027	MsG0180000443.01.T01:CDS
CATCATTTGACTATTTCCAT+AGG	0.472718	1:+6113306	None:intergenic
ATCACCAAAGCTCCAAGACT+TGG	0.492773	1:+6113135	None:intergenic
AGGACTTGAAGTTGAAGAAA+GGG	0.493735	1:-6113186	MsG0180000443.01.T01:CDS
CATTTAGGAAAAGCTTTAAG+TGG	0.499586	1:-6113039	MsG0180000443.01.T01:CDS
AAGGACTTGAAGTTGAAGAA+AGG	0.504271	1:-6113187	MsG0180000443.01.T01:CDS
TACAAAATGTATTCTGTGGA+AGG	0.507359	1:-6113076	MsG0180000443.01.T01:CDS
ATCATTTGACTATTTCCATA+GGG	0.507995	1:+6113307	None:intergenic
TTGTTTGCTGTAATCCTCCA+TGG	0.512879	1:+6113336	None:intergenic
TTTCCTATGCACAAACTCAA+AGG	0.521927	1:-6113222	MsG0180000443.01.T01:CDS
GGCATGTGGTTTATAGCTTG+TGG	0.550765	1:-6113000	MsG0180000443.01.T01:CDS
ATGCAGATGGAGAGCTACAA+TGG	0.572922	1:-6113286	MsG0180000443.01.T01:CDS
AAATAGTCAAATGATGCAGA+TGG	0.594245	1:-6113299	MsG0180000443.01.T01:CDS
AAGGGAAAAGCATTTCATCA+AGG	0.598189	1:-6113168	MsG0180000443.01.T01:CDS
TCTGTGGAAGGAAAAGTGAA+AGG	0.617851	1:-6113064	MsG0180000443.01.T01:CDS
AAAGAATAGGTATTGGCATG+TGG	0.618847	1:-6113014	MsG0180000443.01.T01:CDS
TAGCTACAAAATGTATTCTG+TGG	0.631804	1:-6113080	MsG0180000443.01.T01:CDS
ACCCCTTTGAGTTTGTGCAT+AGG	0.638318	1:+6113219	None:intergenic
TCCTATGCACAAACTCAAAG+GGG	0.640633	1:-6113220	MsG0180000443.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAGACCTAATATCATAATT+AGG	+	Chr1:6113081-6113100	None:intergenic	20.0%
!!	TGAATTTCAAAGAAAGAAAA+GGG	-	Chr1:6113224-6113243	MsG0180000443.01.T01:CDS	20.0%
!	AACTTCAAGTCCTTATTATT+AGG	+	Chr1:6113138-6113157	None:intergenic	25.0%
!	ATCATTTGACTATTTCCATA+GGG	+	Chr1:6113027-6113046	None:intergenic	25.0%
!	CTAAGCCTAATTATGATATT+AGG	-	Chr1:6113073-6113092	MsG0180000443.01.T01:CDS	25.0%
!	CTGAATTTCAAAGAAAGAAA+AGG	-	Chr1:6113223-6113242	MsG0180000443.01.T01:CDS	25.0%
!!!	CTTTTCTTTCTTTGAAATTC+AGG	+	Chr1:6113225-6113244	None:intergenic	25.0%
	AAATAGTCAAATGATGCAGA+TGG	-	Chr1:6113032-6113051	MsG0180000443.01.T01:CDS	30.0%
	AGGATTACAGCAAACAAATT+AGG	-	Chr1:6113001-6113020	MsG0180000443.01.T01:CDS	30.0%
	CATCATTTGACTATTTCCAT+AGG	+	Chr1:6113028-6113047	None:intergenic	30.0%
	GAAAAGTGAAAGGTTCATTT+AGG	-	Chr1:6113277-6113296	MsG0180000443.01.T01:CDS	30.0%
	TACAAAATGTATTCTGTGGA+AGG	-	Chr1:6113255-6113274	MsG0180000443.01.T01:CDS	30.0%
	TAGCTACAAAATGTATTCTG+TGG	-	Chr1:6113251-6113270	MsG0180000443.01.T01:CDS	30.0%
!!	CATTTAGGAAAAGCTTTAAG+TGG	-	Chr1:6113292-6113311	MsG0180000443.01.T01:CDS	30.0%
	AAGGACTTGAAGTTGAAGAA+AGG	-	Chr1:6113144-6113163	MsG0180000443.01.T01:CDS	35.0%
	AGGACTTGAAGTTGAAGAAA+GGG	-	Chr1:6113145-6113164	MsG0180000443.01.T01:CDS	35.0%
	AGTGGCTAAAGAATAGGTAT+TGG	-	Chr1:6113310-6113329	MsG0180000443.01.T01:CDS	35.0%
	TCAAAGGGGTCCTAATAATA+AGG	-	Chr1:6113125-6113144	MsG0180000443.01.T01:CDS	35.0%
	TTCCTATGCACAAACTCAAA+GGG	-	Chr1:6113110-6113129	MsG0180000443.01.T01:CDS	35.0%
	TTTCCTATGCACAAACTCAA+AGG	-	Chr1:6113109-6113128	MsG0180000443.01.T01:CDS	35.0%
!	GCTTTAAGTGGCTAAAGAAT+AGG	-	Chr1:6113304-6113323	MsG0180000443.01.T01:CDS	35.0%
!!	AAAGAATAGGTATTGGCATG+TGG	-	Chr1:6113317-6113336	MsG0180000443.01.T01:CDS	35.0%
!!	AAGGGAAAAGCATTTCATCA+AGG	-	Chr1:6113163-6113182	MsG0180000443.01.T01:CDS	35.0%
	AGCAAACAAATTAGGCCCTA+TGG	-	Chr1:6113009-6113028	MsG0180000443.01.T01:CDS	40.0%
	ATTTCCAAGTCTTGGAGCTT+TGG	-	Chr1:6113192-6113211	MsG0180000443.01.T01:CDS	40.0%
	GGTCTTCAATTTCCAAGTCT+TGG	-	Chr1:6113184-6113203	MsG0180000443.01.T01:CDS	40.0%
	TCCTATGCACAAACTCAAAG+GGG	-	Chr1:6113111-6113130	MsG0180000443.01.T01:CDS	40.0%
	TCTGTGGAAGGAAAAGTGAA+AGG	-	Chr1:6113267-6113286	MsG0180000443.01.T01:CDS	40.0%
	ATCACCAAAGCTCCAAGACT+TGG	+	Chr1:6113199-6113218	None:intergenic	45.0%
	ATGCAGATGGAGAGCTACAA+TGG	-	Chr1:6113045-6113064	MsG0180000443.01.T01:CDS	45.0%
	GGCATGTGGTTTATAGCTTG+TGG	-	Chr1:6113331-6113350	MsG0180000443.01.T01:CDS	45.0%
!	ACCCCTTTGAGTTTGTGCAT+AGG	+	Chr1:6113115-6113134	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	6112997	6113356	6112997	ID=MsG0180000443.01;Name=MsG0180000443.01
Chr1	mRNA	6112997	6113356	6112997	ID=MsG0180000443.01.T01;Parent=MsG0180000443.01;Name=MsG0180000443.01.T01;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|119
Chr1	exon	6112997	6113356	6112997	ID=MsG0180000443.01.T01:exon:27992;Parent=MsG0180000443.01.T01
Chr1	CDS	6112997	6113356	6112997	ID=MsG0180000443.01.T01:cds;Parent=MsG0180000443.01.T01
Chr1	mRNA	6112997	6113311	6112997	ID=MsG0180000443.01.T02;Parent=MsG0180000443.01;Name=MsG0180000443.01.T02;_AED=0.07;_eAED=0.07;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|104
Chr1	exon	6112997	6113311	6112997	ID=MsG0180000443.01.T02:exon:27993;Parent=MsG0180000443.01.T02
Chr1	CDS	6112997	6113311	6112997	ID=MsG0180000443.01.T02:cds;Parent=MsG0180000443.01.T02
Chr1	mRNA	6112997	6113092	6112997	ID=MsG0180000443.01.T03;Parent=MsG0180000443.01;Name=MsG0180000443.01.T03;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|31
Chr1	exon	6112997	6113092	6112997	ID=MsG0180000443.01.T03:exon:27994;Parent=MsG0180000443.01.T03
Chr1	CDS	6112997	6113092	6112997	ID=MsG0180000443.01.T03:cds;Parent=MsG0180000443.01.T03
Chr1	mRNA	6113068	6113136	6113068	ID=MsG0180000443.01.T04;Parent=MsG0180000443.01;Name=MsG0180000443.01.T04;_AED=0.40;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|22
Chr1	exon	6113068	6113136	6113068	ID=MsG0180000443.01.T04:exon:27995;Parent=MsG0180000443.01.T04
Chr1	CDS	6113068	6113136	6113068	ID=MsG0180000443.01.T04:cds;Parent=MsG0180000443.01.T04

Gene Sequence

>MsG0180000443.01.T04

ATGATCCTGAATTTCAAAGAAAGAAAAGGGTTGCTAGCTACAAAATGTATTCTGTGGAAGGAAAAGTGA

>MsG0180000443.01.T01

ATGGAGGATTACAGCAAACAAATTAGGCCCTATGGAAATAGTCAAATGATGCAGATGGAGAGCTACAATGGAGCAACTAAGCCTAATTATGATATTAGGTCTTATAGTGATTTTTCCTATGCACAAACTCAAAGGGGTCCTAATAATAAGGACTTGAAGTTGAAGAAAGGGAAAAGCATTTCATCAAGGTCTTCAATTTCCAAGTCTTGGAGCTTTGGTGATGATCCTGAATTTCAAAGAAAGAAAAGGGTTGCTAGCTACAAAATGTATTCTGTGGAAGGAAAAGTGAAAGGTTCATTTAGGAAAAGCTTTAAGTGGCTAAAGAATAGGTATTGGCATGTGGTTTATAGCTTGTGGTGA

>MsG0180000443.01.T03

ATGTATTCTGTGGAAGGAAAAGTGAAAGGTTCATTTAGGAAAAGCTTTAAGTGGCTAAAGAATAGGTATTGGCATGTGGTTTATAGCTTGTGGTGA

>MsG0180000443.01.T02

ATGATGCAGATGGAGAGCTACAATGGAGCAACTAAGCCTAATTATGATATTAGGTCTTATAGTGATTTTTCCTATGCACAAACTCAAAGGGGTCCTAATAATAAGGACTTGAAGTTGAAGAAAGGGAAAAGCATTTCATCAAGGTCTTCAATTTCCAAGTCTTGGAGCTTTGGTGATGATCCTGAATTTCAAAGAAAGAAAAGGGTTGCTAGCTACAAAATGTATTCTGTGGAAGGAAAAGTGAAAGGTTCATTTAGGAAAAGCTTTAAGTGGCTAAAGAATAGGTATTGGCATGTGGTTTATAGCTTGTGGTGA

Protein sequence

>MsG0180000443.01.T04

MILNFKERKGLLATKCILWKEK*

>MsG0180000443.01.T01

MEDYSKQIRPYGNSQMMQMESYNGATKPNYDIRSYSDFSYAQTQRGPNNKDLKLKKGKSISSRSSISKSWSFGDDPEFQRKKRVASYKMYSVEGKVKGSFRKSFKWLKNRYWHVVYSLW*

>MsG0180000443.01.T03

MYSVEGKVKGSFRKSFKWLKNRYWHVVYSLW*

>MsG0180000443.01.T02

MMQMESYNGATKPNYDIRSYSDFSYAQTQRGPNNKDLKLKKGKSISSRSSISKSWSFGDDPEFQRKKRVASYKMYSVEGKVKGSFRKSFKWLKNRYWHVVYSLW*