Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001462.01.T01 | XP_024637399.2 | 85.774 | 717 | 99 | 1 | 1 | 717 | 1 | 714 | 0 | 1243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001462.01.T01 | A0A396JPT3 | 85.774 | 717 | 99 | 1 | 1 | 717 | 1 | 714 | 0.0 | 1242 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001442.01 | MsG0180001462.01 | 0.825560 | 4.386364e-54 | 4.011843e-51 |
| MsG0180001462.01 | MsG0280010675.01 | 0.801104 | 1.054126e-48 | 4.992117e-46 |
| MsG0180001462.01 | MsG0480020229.01 | 0.803233 | 3.844199e-49 | 1.921611e-46 |
| MsG0180001462.01 | MsG0880045409.01 | 0.806926 | 6.487302e-50 | 3.565547e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001462.01.T01 | MTR_1g051585 | 84.979 | 719 | 105 | 2 | 1 | 717 | 1 | 718 | 0.0 | 1241 |
| MsG0180001462.01.T01 | MTR_0068s0080 | 85.933 | 718 | 96 | 3 | 1 | 717 | 1 | 714 | 0.0 | 1239 |
| MsG0180001462.01.T01 | MTR_0068s0080 | 85.933 | 718 | 96 | 3 | 1 | 717 | 1 | 714 | 0.0 | 1237 |
| MsG0180001462.01.T01 | MTR_1g036800 | 85.237 | 718 | 102 | 2 | 1 | 717 | 1 | 715 | 0.0 | 1230 |
| MsG0180001462.01.T01 | MTR_2g089975 | 82.893 | 719 | 120 | 2 | 1 | 717 | 1 | 718 | 0.0 | 1212 |
| MsG0180001462.01.T01 | MTR_1g041550 | 82.361 | 720 | 123 | 3 | 1 | 717 | 1 | 719 | 0.0 | 1207 |
| MsG0180001462.01.T01 | MTR_0069s0040 | 84.145 | 719 | 111 | 2 | 1 | 717 | 1 | 718 | 0.0 | 1199 |
| MsG0180001462.01.T01 | MTR_1g037130 | 82.197 | 719 | 125 | 2 | 1 | 717 | 1 | 718 | 0.0 | 1196 |
| MsG0180001462.01.T01 | MTR_1g037250 | 82.069 | 725 | 121 | 4 | 1 | 717 | 1 | 724 | 0.0 | 1183 |
| MsG0180001462.01.T01 | MTR_8g059345 | 81.641 | 719 | 127 | 2 | 1 | 717 | 1 | 716 | 0.0 | 1179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001462.01.T01 | AT4G27190 | 27.831 | 733 | 482 | 21 | 1 | 706 | 1 | 713 | 5.78e-67 | 239 |
| MsG0180001462.01.T01 | AT4G27220 | 26.309 | 726 | 476 | 19 | 24 | 719 | 5 | 701 | 1.06e-64 | 231 |
| MsG0180001462.01.T01 | AT4G27220 | 26.454 | 722 | 472 | 19 | 28 | 719 | 1 | 693 | 1.15e-63 | 229 |
| MsG0180001462.01.T01 | AT4G26090 | 26.987 | 541 | 352 | 14 | 158 | 681 | 160 | 674 | 1.80e-50 | 190 |
| MsG0180001462.01.T01 | AT5G05400 | 27.734 | 631 | 417 | 18 | 35 | 644 | 38 | 650 | 4.53e-50 | 189 |
| MsG0180001462.01.T01 | AT1G12280 | 26.498 | 634 | 429 | 15 | 19 | 623 | 18 | 643 | 1.78e-44 | 172 |
| MsG0180001462.01.T01 | AT1G12220 | 27.410 | 664 | 438 | 19 | 7 | 642 | 14 | 661 | 3.47e-44 | 171 |
| MsG0180001462.01.T01 | AT1G12220 | 27.410 | 664 | 438 | 19 | 7 | 642 | 14 | 661 | 3.47e-44 | 171 |
| MsG0180001462.01.T01 | AT1G12220 | 29.144 | 549 | 352 | 16 | 119 | 642 | 4 | 540 | 3.66e-42 | 164 |
| MsG0180001462.01.T01 | AT1G12290 | 25.776 | 644 | 398 | 19 | 20 | 602 | 23 | 647 | 5.64e-42 | 164 |
Find 152 sgRNAs with CRISPR-Local
Find 181 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTGATATGATGTTGGG+TGG | 0.678070 | 1:-21698766 | None:intergenic |
| AAAGAAGTGGGGATACCAGT+TGG | 0.591163 | 1:+21697680 | MsG0180001462.01.T01:CDS |
| AAAGATGTTGTTCAAGTTCA+AGG | 0.361031 | 1:+21697236 | MsG0180001462.01.T01:CDS |
| AAATAAATTGTTCCATAAAG+TGG | 0.498156 | 1:+21697475 | MsG0180001462.01.T01:CDS |
| AAATAGCTATTGGAGATGTA+TGG | 0.413958 | 1:+21698986 | MsG0180001462.01.T01:CDS |
| AAATGGTAAAGAAATTGAGA+AGG | 0.650768 | 1:+21697058 | MsG0180001462.01.T01:CDS |
| AAATTGGAGGAGTTGTACAT+GGG | 0.691550 | 1:+21698793 | MsG0180001462.01.T01:CDS |
| AACATCAATCGTAAGTCCCT+TGG | 0.516792 | 1:-21698943 | None:intergenic |
| AACATTGGGGTCTATGGGTT+GGG | 0.431837 | 1:+21697401 | MsG0180001462.01.T01:CDS |
| AACTCTAGAGCTGTCAAGTT+GGG | 0.483693 | 1:-21698895 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAGCTATTGGAGATGTA+TGG | + | Chr1:21698986-21699005 | MsG0180001462.01.T01:CDS | 30.0% | |
| AAGTACTTCATATCATTACC+TGG | - | Chr1:21698115-21698134 | None:intergenic | 30.0% | |
| AATAGCTATTGGAGATGTAT+GGG | + | Chr1:21698987-21699006 | MsG0180001462.01.T01:CDS | 30.0% | |
| AATCCAAACACAATGTTTGA+AGG | - | Chr1:21698591-21698610 | None:intergenic | 30.0% | |
| ACTTTGAATGTGAAATCCTT+TGG | - | Chr1:21697776-21697795 | None:intergenic | 30.0% | |
| AGACATCAAAAGAATTCAAG+GGG | + | Chr1:21697523-21697542 | MsG0180001462.01.T01:CDS | 30.0% | |
| AGTCAAACTTGATATGATGT+TGG | - | Chr1:21698773-21698792 | None:intergenic | 30.0% | |
| ATCAAGTTTGACTAAATTGG+AGG | + | Chr1:21698780-21698799 | MsG0180001462.01.T01:CDS | 30.0% | |
| ATGTAACTTCAAGACGTTAA+AGG | + | Chr1:21696971-21696990 | MsG0180001462.01.T01:CDS | 30.0% | |
| ATGTTAAATCTAGCACTCTA+AGG | - | Chr1:21698522-21698541 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 21696882 | 21699047 | 21696882 | ID=MsG0180001462.01;Name=MsG0180001462.01 |
| Chr1 | mRNA | 21696882 | 21699047 | 21696882 | ID=MsG0180001462.01.T01;Parent=MsG0180001462.01;Name=MsG0180001462.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|721 |
| Chr1 | exon | 21696882 | 21699047 | 21696882 | ID=MsG0180001462.01.T01:exon:13689;Parent=MsG0180001462.01.T01 |
| Chr1 | CDS | 21696882 | 21699047 | 21696882 | ID=MsG0180001462.01.T01:cds;Parent=MsG0180001462.01.T01 |
| Gene Sequence |
>MsG0180001462.01.T01 ATGGAAATTTTAATTTCTGTTGTTGCAAAAATAGCAGAATATACCGTTGTGCCTATTGCACGTCAAGCAAGTTACTCGATATTCTACAAATGTAACTTCAAGACGTTAAAGGATGATGTTGAAGACCTTGAGGCTGCAAGAGCAAGAATGATCCATTCGGTTGAAAGAGAAAGGGGAAATGGTAAAGAAATTGAGAAGGATGTTTTGAATTGGTTGGAGAAAGTGAATGAGGTCATTGAAAAGGCAAATGGGCTTCAAAACGATCCTCGCCGTGACAATGTTAGGTGCTCAGCATGGTCGTTCCCCAACTTGATTTTACGTCATCAACTAAGCAGGAAAGCCACAAAAATTGCAAAAGATGTTGTTCAAGTTCAAGGAAAAGGGATTTTTGATCAAGTTGGTTACCTTCCCCCTCTAGATGGAGTACCCTCCTCCTCAACAAGAGATGGTGAAATGTATGACACAAGGGAGTCTCTTAAGGAGGACATTGTGAACGTTTTAAAAGATCCTACTTCACGCAACATTGGGGTCTATGGGTTGGGTGGGGTGGGCAAGACCACTATAGTGGAGAAAGTTGCTCAACTAGCCAAGGAAAATAAATTGTTCCATAAAGTGGTGAAGACAGAAGTATCCAAAATTCCAGACATCAAAAGAATTCAAGGGGAGATTGCAGACTTCTTGGGTCTAAAATTCGAAGAGGAGACTATTCTTGGCAGAGCACAACGCCTAAGACAAAGTATCCAGATGGAGAAAAGTATCCTTATCATTCTAGATAACATATGGACCATACTTGATTTGAAAGAAGTGGGGATACCAGTTGGTGATGAACATAATGGTTGCAAATTGTTGATGACATGCAGAAATCAAGAAGTGTTGTTGCAAATGGATGTTCCAAAGGATTTCACATTCAAAGTTGAACTTATGAGTGAAAAAGAGACATGGAGCTTGTTTCAATTTATGGCAGGGGATGTGGTTAAAGATAGCAATTTGAAAGATCTACCATTTCAAGTGGCCAGAAAATGTGCAGGTTTGCCTCTAAGGGTAGTGACAGTAGCGCGTGCAATGAAAAATAAAAGGGATATCCAGTCTTGGAAAAATGCATTCAGACAATTACAAAGTAATGATCATACAGATCAGATGGATGCAGAAACTTATTCTGCTTTGGAATTAAGTTACAACTCATTGCATAGTGATGAAATGAGGGCTCTCTTCTTACTTTTTGCATCACTTCCAGGTAATGATATGAAGTACTTTCTCCAGGTTTCAATGGGGTTAGACATATTGAAGCATACCGTGGATGACGCAAGAAACAAACTTTTCACAATAATCAAATCTTTGGAGGCAACTTGTCTTTTGCATGAAGTAAAAACAGATGCAAAGATCCAAATGCATGACTTTGTTCGTGATTTTGCTATCTCCATAGCACTTAGGGACAAACATGTGTTTATAAGAAAACAATCTGATGAGGAATGGCCAACTGAGGATTTTTTGAAAAGGTGCACACAGATCGTTCTTGATAGATGTTATATGCACGAGCTTCCTCAAATGATTGATTGTCCAAACATTAAGTTTTTCTGTTTGGGCAGTAAGAATCAAACATTAGAAATCCCTGATACTTTTTTTGAGGGTATGAAAAGCCTTAGAGTGCTAGATTTAACATACTTGAACTTGTCATCATTACCCAAATCATTCCAGTTACTAATAGACCTTCAAACATTGTGTTTGGATTATTGCATTTTGGAAAATATGGATGCAATAGAAGCTTTGCAAAATTTAGAAATTCTTCGCCTCTATAAATCCTCAATGATCAAGTTGCCTAGAGAAATAGGGAGATTGATTCGATTGAGAATGCTTGACTTGAGCCATTCAGGAATAGAAGTAGTTCCACCCAACATCATATCAAGTTTGACTAAATTGGAGGAGTTGTACATGGGAAATACCTCTATTAATTGGGAAGATGTGAATTCAACAGTTCAAAGTGAACACGCTAGTATTGCTGAGCTTCGAAAGCTACCCAACTTGACAGCTCTAGAGTTACAAATTCGTGAGACTTGGATGTTGCCAAGGGACTTACGATTGATGTTTGAGAACCTGGAAAGATATAAAATAGCTATTGGAGATGTATGGGACTGGTCTGACATTAAGGATGGCAGGGGCGAATGGTAG |
| Protein sequence |
>MsG0180001462.01.T01 MEILISVVAKIAEYTVVPIARQASYSIFYKCNFKTLKDDVEDLEAARARMIHSVERERGNGKEIEKDVLNWLEKVNEVIEKANGLQNDPRRDNVRCSAWSFPNLILRHQLSRKATKIAKDVVQVQGKGIFDQVGYLPPLDGVPSSSTRDGEMYDTRESLKEDIVNVLKDPTSRNIGVYGLGGVGKTTIVEKVAQLAKENKLFHKVVKTEVSKIPDIKRIQGEIADFLGLKFEEETILGRAQRLRQSIQMEKSILIILDNIWTILDLKEVGIPVGDEHNGCKLLMTCRNQEVLLQMDVPKDFTFKVELMSEKETWSLFQFMAGDVVKDSNLKDLPFQVARKCAGLPLRVVTVARAMKNKRDIQSWKNAFRQLQSNDHTDQMDAETYSALELSYNSLHSDEMRALFLLFASLPGNDMKYFLQVSMGLDILKHTVDDARNKLFTIIKSLEATCLLHEVKTDAKIQMHDFVRDFAISIALRDKHVFIRKQSDEEWPTEDFLKRCTQIVLDRCYMHELPQMIDCPNIKFFCLGSKNQTLEIPDTFFEGMKSLRVLDLTYLNLSSLPKSFQLLIDLQTLCLDYCILENMDAIEALQNLEILRLYKSSMIKLPREIGRLIRLRMLDLSHSGIEVVPPNIISSLTKLEELYMGNTSINWEDVNSTVQSEHASIAELRKLPNLTALELQIRETWMLPRDLRLMFENLERYKIAIGDVWDWSDIKDGRGEW* |
