Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001692.01.T01 | XP_024626489.1 | 89.6 | 125 | 13 | 0 | 75 | 199 | 1085 | 1209 | 1.33E-65 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001692.01.T01 | A0A072VGV5 | 89.600 | 125 | 13 | 0 | 75 | 199 | 1085 | 1209 | 6.37e-66 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001692.01 | MsG0380015548.01 | 0.803241 | 3.828883e-49 | 1.914371e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001692.01.T01 | MTR_1g041300 | 89.600 | 125 | 13 | 0 | 75 | 199 | 1085 | 1209 | 1.62e-69 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGAAAGCCTTAAGATTT+TGG | 0.090861 | 1:+25397441 | None:intergenic |
| CAAGTGATGTTTGGATTATC+AGG | 0.272548 | 1:+25397047 | None:intergenic |
| TGAAGAGAAAATTGTTACTA+TGG | 0.288677 | 1:-25396859 | MsG0180001692.01.T01:CDS |
| ACCCAAAATCTTAAGGCTTT+CGG | 0.289769 | 1:-25397443 | MsG0180001692.01.T01:CDS |
| GTCCGGTCTCTTGGTGATCC+TGG | 0.364668 | 1:+25397477 | None:intergenic |
| CATTTCAACCCAAAATCTTA+AGG | 0.374331 | 1:-25397450 | MsG0180001692.01.T01:CDS |
| CTTCTTGTCTTGATTCAGTT+CGG | 0.380317 | 1:+25396988 | None:intergenic |
| TGCATCTGACAAGTGATGTT+TGG | 0.400511 | 1:+25397038 | None:intergenic |
| CAAATACCGTTCCAGCTGTC+GGG | 0.412049 | 1:+25397354 | None:intergenic |
| AACAATTCTACATTTGCAAA+TGG | 0.420420 | 1:-25397119 | MsG0180001692.01.T01:CDS |
| GAAGAAGCAATTGAGAAAGA+AGG | 0.449564 | 1:-25397216 | MsG0180001692.01.T01:CDS |
| AAATGTGGTGTCCGGTCTCT+TGG | 0.461743 | 1:+25397468 | None:intergenic |
| AAGAAGCAATTGAGAAAGAA+GGG | 0.472715 | 1:-25397215 | MsG0180001692.01.T01:CDS |
| TGAATCAAGACAAGAAGAAT+CGG | 0.484023 | 1:-25396982 | MsG0180001692.01.T01:CDS |
| TTGGGTTGAAATGTGGTGTC+CGG | 0.493063 | 1:+25397460 | None:intergenic |
| CAAATGGTGGTTATGAAACA+AGG | 0.500081 | 1:-25397103 | MsG0180001692.01.T01:CDS |
| TCAGTGCCTCATGATGAGTA+TGG | 0.502689 | 1:-25396888 | MsG0180001692.01.T01:CDS |
| TTTCTGAACCACCGAGAGAT+CGG | 0.510422 | 1:+25397405 | None:intergenic |
| ATATCTAAAGCTTCGTTCGT+TGG | 0.510865 | 1:-25396819 | MsG0180001692.01.T01:CDS |
| AGAAGCAGCTTCACTGATAG+TGG | 0.511751 | 1:-25397173 | MsG0180001692.01.T01:CDS |
| TCTCGAGACTCCCCGACAGC+TGG | 0.512920 | 1:-25397365 | MsG0180001692.01.T01:CDS |
| TTCTGAACCACCGAGAGATC+GGG | 0.520899 | 1:+25397406 | None:intergenic |
| GACATGGTGTCCCGATCTCT+CGG | 0.524228 | 1:-25397416 | MsG0180001692.01.T01:CDS |
| AAGAATGAGCTAAATGATCC+AGG | 0.525342 | 1:-25397495 | None:intergenic |
| AATTCTACATTTGCAAATGG+TGG | 0.527425 | 1:-25397116 | MsG0180001692.01.T01:CDS |
| GGAGTCTCGAGAGCAACAAC+GGG | 0.536250 | 1:+25397376 | None:intergenic |
| CGGAGGTTGAGATTGAAGCT+TGG | 0.536638 | 1:+25397008 | None:intergenic |
| ATCCAGGATCACCAAGAGAC+CGG | 0.541341 | 1:-25397479 | MsG0180001692.01.T01:CDS |
| ATTGTTTGAACTTAATTCAG+TGG | 0.543504 | 1:+25397136 | None:intergenic |
| CCAAATACCGTTCCAGCTGT+CGG | 0.551035 | 1:+25397353 | None:intergenic |
| CTTGTCTTGATTCAGTTCGG+AGG | 0.568795 | 1:+25396991 | None:intergenic |
| GGGAGTCTCGAGAGCAACAA+CGG | 0.580719 | 1:+25397375 | None:intergenic |
| TAAGGCTTTCGGAGGAGACA+TGG | 0.581347 | 1:-25397432 | MsG0180001692.01.T01:CDS |
| TCTGAAGACTTATCAAGCAA+TGG | 0.583115 | 1:+25396951 | None:intergenic |
| AGATGATAGCTTGTCAAACA+AGG | 0.589755 | 1:-25396931 | MsG0180001692.01.T01:CDS |
| AGACTCCCCGACAGCTGGAA+CGG | 0.592227 | 1:-25397360 | MsG0180001692.01.T01:intron |
| AAATACCGTTCCAGCTGTCG+GGG | 0.609455 | 1:+25397355 | None:intergenic |
| CAAAATCTTAAGGCTTTCGG+AGG | 0.622089 | 1:-25397440 | MsG0180001692.01.T01:CDS |
| ATGAGACCATACTCATCATG+AGG | 0.664138 | 1:+25396882 | None:intergenic |
| AAACAAGGGAGAGTGTAACA+AGG | 0.685981 | 1:-25397088 | MsG0180001692.01.T01:CDS |
| AAATGGTGGTTATGAAACAA+GGG | 0.692406 | 1:-25397102 | MsG0180001692.01.T01:CDS |
| ATGGTGTCCCGATCTCTCGG+TGG | 0.717382 | 1:-25397413 | MsG0180001692.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTAACAATTCAATATA+TGG | - | Chr1:25397017-25397036 | MsG0180001692.01.T01:CDS | 15.0% |
| !!! | GTTTTTTAAAAGAAGAAAAA+GGG | - | Chr1:25397470-25397489 | MsG0180001692.01.T01:CDS | 15.0% |
| !!! | GGTTTTTTAAAAGAAGAAAA+AGG | - | Chr1:25397469-25397488 | MsG0180001692.01.T01:CDS | 20.0% |
| ! | AACAATTCTACATTTGCAAA+TGG | - | Chr1:25397148-25397167 | MsG0180001692.01.T01:CDS | 25.0% |
| ! | ATTGTTTGAACTTAATTCAG+TGG | + | Chr1:25397134-25397153 | None:intergenic | 25.0% |
| ! | TGAAGAGAAAATTGTTACTA+TGG | - | Chr1:25397408-25397427 | MsG0180001692.01.T01:CDS | 25.0% |
| AAATGGTGGTTATGAAACAA+GGG | - | Chr1:25397165-25397184 | MsG0180001692.01.T01:CDS | 30.0% | |
| AAGAAGCAATTGAGAAAGAA+GGG | - | Chr1:25397052-25397071 | MsG0180001692.01.T01:CDS | 30.0% | |
| AATTCTACATTTGCAAATGG+TGG | - | Chr1:25397151-25397170 | MsG0180001692.01.T01:CDS | 30.0% | |
| CATTTCAACCCAAAATCTTA+AGG | - | Chr1:25396817-25396836 | MsG0180001692.01.T01:CDS | 30.0% | |
| TGAATCAAGACAAGAAGAAT+CGG | - | Chr1:25397285-25397304 | MsG0180001692.01.T01:intron | 30.0% | |
| !!! | TAAGATTTTGGGTTGAAATG+TGG | + | Chr1:25396817-25396836 | None:intergenic | 30.0% |
| ACCCAAAATCTTAAGGCTTT+CGG | - | Chr1:25396824-25396843 | MsG0180001692.01.T01:CDS | 35.0% | |
| CAAATGGTGGTTATGAAACA+AGG | - | Chr1:25397164-25397183 | MsG0180001692.01.T01:CDS | 35.0% | |
| CAAGTGATGTTTGGATTATC+AGG | + | Chr1:25397223-25397242 | None:intergenic | 35.0% | |
| CTTCTTGTCTTGATTCAGTT+CGG | + | Chr1:25397282-25397301 | None:intergenic | 35.0% | |
| GAAGAAGCAATTGAGAAAGA+AGG | - | Chr1:25397051-25397070 | MsG0180001692.01.T01:CDS | 35.0% | |
| TCTGAAGACTTATCAAGCAA+TGG | + | Chr1:25397319-25397338 | None:intergenic | 35.0% | |
| ! | AGATGATAGCTTGTCAAACA+AGG | - | Chr1:25397336-25397355 | MsG0180001692.01.T01:intron | 35.0% |
| ! | ATATCTAAAGCTTCGTTCGT+TGG | - | Chr1:25397448-25397467 | MsG0180001692.01.T01:CDS | 35.0% |
| ! | TCCGAAAGCCTTAAGATTTT+GGG | + | Chr1:25396828-25396847 | None:intergenic | 35.0% |
| ATGAGACCATACTCATCATG+AGG | + | Chr1:25397388-25397407 | None:intergenic | 40.0% | |
| CAAAATCTTAAGGCTTTCGG+AGG | - | Chr1:25396827-25396846 | MsG0180001692.01.T01:CDS | 40.0% | |
| TGCATCTGACAAGTGATGTT+TGG | + | Chr1:25397232-25397251 | None:intergenic | 40.0% | |
| ! | AAACAAGGGAGAGTGTAACA+AGG | - | Chr1:25397179-25397198 | MsG0180001692.01.T01:CDS | 40.0% |
| ! | CTCCGAAAGCCTTAAGATTT+TGG | + | Chr1:25396829-25396848 | None:intergenic | 40.0% |
| AGAAGCAGCTTCACTGATAG+TGG | - | Chr1:25397094-25397113 | MsG0180001692.01.T01:CDS | 45.0% | |
| CTTGTCTTGATTCAGTTCGG+AGG | + | Chr1:25397279-25397298 | None:intergenic | 45.0% | |
| TCAGTGCCTCATGATGAGTA+TGG | - | Chr1:25397379-25397398 | MsG0180001692.01.T01:CDS | 45.0% | |
| TTTCTGAACCACCGAGAGAT+CGG | + | Chr1:25396865-25396884 | None:intergenic | 45.0% | |
| !! | TTGGGTTGAAATGTGGTGTC+CGG | + | Chr1:25396810-25396829 | None:intergenic | 45.0% |
| AAATACCGTTCCAGCTGTCG+GGG | + | Chr1:25396915-25396934 | None:intergenic | 50.0% | |
| AAATGTGGTGTCCGGTCTCT+TGG | + | Chr1:25396802-25396821 | None:intergenic | 50.0% | |
| ATCCAGGATCACCAAGAGAC+CGG | - | Chr1:25396788-25396807 | MsG0180001692.01.T01:CDS | 50.0% | |
| CAAATACCGTTCCAGCTGTC+GGG | + | Chr1:25396916-25396935 | None:intergenic | 50.0% | |
| CCAAATACCGTTCCAGCTGT+CGG | + | Chr1:25396917-25396936 | None:intergenic | 50.0% | |
| CGGAGGTTGAGATTGAAGCT+TGG | + | Chr1:25397262-25397281 | None:intergenic | 50.0% | |
| TAAGGCTTTCGGAGGAGACA+TGG | - | Chr1:25396835-25396854 | MsG0180001692.01.T01:CDS | 50.0% | |
| TTCTGAACCACCGAGAGATC+GGG | + | Chr1:25396864-25396883 | None:intergenic | 50.0% | |
| CCGACAGCTGGAACGGTATT+TGG | - | Chr1:25396914-25396933 | MsG0180001692.01.T01:CDS | 55.0% | |
| GACATGGTGTCCCGATCTCT+CGG | - | Chr1:25396851-25396870 | MsG0180001692.01.T01:CDS | 55.0% | |
| GGAGTCTCGAGAGCAACAAC+GGG | + | Chr1:25396894-25396913 | None:intergenic | 55.0% | |
| GGGAGTCTCGAGAGCAACAA+CGG | + | Chr1:25396895-25396914 | None:intergenic | 55.0% | |
| AGACTCCCCGACAGCTGGAA+CGG | - | Chr1:25396907-25396926 | MsG0180001692.01.T01:CDS | 60.0% | |
| ATGGTGTCCCGATCTCTCGG+TGG | - | Chr1:25396854-25396873 | MsG0180001692.01.T01:CDS | 60.0% | |
| ! | GTCCGGTCTCTTGGTGATCC+TGG | + | Chr1:25396793-25396812 | None:intergenic | 60.0% |
| TCTCGAGACTCCCCGACAGC+TGG | - | Chr1:25396902-25396921 | MsG0180001692.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 25396785 | 25397504 | 25396785 | ID=MsG0180001692.01;Name=MsG0180001692.01 |
| Chr1 | mRNA | 25396785 | 25397504 | 25396785 | ID=MsG0180001692.01.T01;Parent=MsG0180001692.01;Name=MsG0180001692.01.T01;_AED=0.60;_eAED=0.64;_QI=0|0|0|0.5|1|1|2|0|199 |
| Chr1 | exon | 25397361 | 25397504 | 25397361 | ID=MsG0180001692.01.T01:exon:40838;Parent=MsG0180001692.01.T01 |
| Chr1 | exon | 25396785 | 25397240 | 25396785 | ID=MsG0180001692.01.T01:exon:40837;Parent=MsG0180001692.01.T01 |
| Chr1 | CDS | 25397361 | 25397504 | 25397361 | ID=MsG0180001692.01.T01:cds;Parent=MsG0180001692.01.T01 |
| Chr1 | CDS | 25396785 | 25397240 | 25396785 | ID=MsG0180001692.01.T01:cds;Parent=MsG0180001692.01.T01 |
| Gene Sequence |
| Protein sequence |
