AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002270.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002270.01.T01 MTR_7g065630 95.968 124 5 0 1 124 1 124 1.69e-84 249
MsG0180002270.01.T01 MTR_7g065680 84.545 110 16 1 19 127 1 110 2.61e-62 192
MsG0180002270.01.T01 MTR_7g065700 84.906 106 16 0 19 124 1 106 4.13e-61 189
MsG0180002270.01.T01 MTR_7g065710 84.848 99 15 0 26 124 5 103 2.84e-58 182
MsG0180002270.01.T01 MTR_7g065230 77.358 106 24 0 19 124 1 106 7.76e-56 175
MsG0180002270.01.T01 MTR_7g065720 74.312 109 28 0 19 127 1 109 1.85e-55 174
MsG0180002270.01.T01 MTR_7g065590 72.727 110 29 1 19 127 1 110 9.37e-55 172
MsG0180002270.01.T01 MTR_7g065265 74.528 106 27 0 19 124 1 106 1.10e-53 170
MsG0180002270.01.T01 MTR_7g065640 72.477 109 30 0 19 127 1 109 3.30e-53 168
MsG0180002270.01.T01 MTR_7g065600 72.642 106 29 0 19 124 1 106 1.65e-52 167
MsG0180002270.01.T01 MTR_7g065660 71.560 109 31 0 19 127 1 109 2.45e-52 167
MsG0180002270.01.T01 MTR_7g065290 71.698 106 30 0 19 124 1 106 9.79e-52 165
MsG0180002270.01.T01 MTR_7g065270 71.698 106 30 0 19 124 1 106 2.94e-51 164
MsG0180002270.01.T01 MTR_7g065260 68.807 109 34 0 19 127 8 116 6.96e-51 163
MsG0180002270.01.T01 MTR_7g065750 69.811 106 32 0 19 124 1 106 3.63e-50 161
MsG0180002270.01.T01 MTR_7g065740 66.972 109 36 0 19 127 1 109 9.04e-49 157
MsG0180002270.01.T01 MTR_4g019780 65.421 107 37 0 18 124 1 107 4.01e-48 155
MsG0180002270.01.T01 MTR_4g019790 66.981 106 35 0 19 124 1 106 5.37e-46 150
MsG0180002270.01.T01 MTR_3g099757 60.526 114 41 1 22 131 2 115 3.88e-44 145
MsG0180002270.01.T01 MTR_6g080440 57.798 109 45 1 25 132 3 111 5.54e-37 127
MsG0180002270.01.T01 MTR_8g087425 58.416 101 42 0 23 123 3 103 3.80e-36 125
MsG0180002270.01.T01 MTR_1g090070 56.863 102 44 0 25 126 9 110 4.02e-35 122
MsG0180002270.01.T01 MTR_1g090150 54.455 101 46 0 24 124 2 102 2.22e-34 120
MsG0180002270.01.T01 MTR_5g040430 50.909 110 53 1 19 127 1 110 2.55e-34 120
MsG0180002270.01.T01 MTR_0186s0030 54.902 102 45 1 23 123 2 103 5.45e-34 117
MsG0180002270.01.T01 MTR_4g059730 52.336 107 51 0 23 129 2 108 7.05e-34 119
MsG0180002270.01.T01 MTR_4g124130 58.252 103 41 2 24 124 3 105 1.83e-33 118
MsG0180002270.01.T01 MTR_8g056940 55.882 102 44 1 23 123 2 103 2.01e-33 118
MsG0180002270.01.T01 MTR_8g087410 56.190 105 46 0 24 128 5 109 2.03e-33 118
MsG0180002270.01.T01 MTR_1g115195 57.426 101 43 0 25 125 3 103 3.39e-33 117
MsG0180002270.01.T01 MTR_1g090060 52.381 105 50 0 25 129 3 107 2.60e-32 115
MsG0180002270.01.T01 MTR_2g070150 52.941 102 47 1 23 123 2 103 7.95e-32 114
MsG0180002270.01.T01 MTR_2g070130 50.000 110 54 1 23 131 2 111 8.58e-32 114
MsG0180002270.01.T01 MTR_1g090100 54.545 99 45 0 26 124 3 101 1.70e-31 113
MsG0180002270.01.T01 MTR_2g070210 54.808 104 44 2 23 123 2 105 3.64e-31 112
MsG0180002270.01.T01 MTR_2g070070 50.000 102 50 1 23 123 2 103 5.44e-31 112
MsG0180002270.01.T01 MTR_8g061950 46.763 139 69 3 25 160 5 141 5.58e-31 112
MsG0180002270.01.T01 MTR_2g070180 50.980 102 49 1 23 123 2 103 1.18e-30 111
MsG0180002270.01.T01 MTR_2g070200 51.961 102 48 1 23 123 2 103 2.92e-30 110
MsG0180002270.01.T01 MTR_5g037380 48.113 106 52 1 22 124 2 107 5.95e-30 109
MsG0180002270.01.T01 MTR_3g467420 52.525 99 46 1 26 123 10 108 2.18e-29 108
MsG0180002270.01.T01 MTR_2g070120 50.980 102 49 1 23 123 2 103 4.66e-29 107
MsG0180002270.01.T01 MTR_1g090090 52.525 99 47 0 25 123 3 101 4.72e-29 107
MsG0180002270.01.T01 MTR_2g070140 52.874 87 40 1 38 123 1 87 4.63e-28 103
MsG0180002270.01.T01 MTR_3g467430 49.515 103 51 1 22 123 6 108 6.13e-28 104
MsG0180002270.01.T01 MTR_5g076900 50.000 104 51 1 21 123 2 105 2.70e-27 102
MsG0180002270.01.T01 MTR_2g070060 47.059 102 53 1 23 123 2 103 4.30e-27 102
MsG0180002270.01.T01 MTR_2g070110 48.039 102 52 1 23 123 2 103 7.64e-27 101
MsG0180002270.01.T01 MTR_2g072120 42.857 56 31 1 69 123 2 57 3.41e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002270.01.T01 AT2G29420 61.538 104 40 0 21 124 5 108 1.32e-44 147
MsG0180002270.01.T01 AT2G29490 55.372 121 53 1 19 139 1 120 9.53e-41 137
MsG0180002270.01.T01 AT2G29450 61.468 109 42 0 21 129 2 110 3.24e-39 133
MsG0180002270.01.T01 AT2G29480 55.372 121 53 1 19 139 1 120 1.10e-38 132
MsG0180002270.01.T01 AT2G29460 56.604 106 46 0 19 124 1 106 1.39e-37 129
MsG0180002270.01.T01 AT3G09270 58.333 108 43 2 24 129 5 112 1.84e-37 129
MsG0180002270.01.T01 AT2G29440 53.279 122 56 1 24 145 5 125 3.42e-37 128
MsG0180002270.01.T01 AT2G29470 52.459 122 56 2 19 139 1 121 3.61e-37 128
MsG0180002270.01.T01 AT1G10370 42.484 153 85 2 22 171 2 154 6.85e-35 122
MsG0180002270.01.T01 AT5G62480 56.566 99 42 1 25 122 8 106 4.00e-34 120
MsG0180002270.01.T01 AT1G59670 56.075 107 44 2 24 127 5 111 8.04e-34 119
MsG0180002270.01.T01 AT1G59700 58.654 104 40 2 24 124 5 108 1.01e-33 119
MsG0180002270.01.T01 AT1G78340 53.922 102 46 1 23 123 2 103 1.09e-33 119
MsG0180002270.01.T01 AT1G10360 41.830 153 86 2 22 171 2 154 1.98e-32 115
MsG0180002270.01.T01 AT1G69930 49.123 114 56 2 20 131 7 120 6.24e-32 115
MsG0180002270.01.T01 AT1G17170 52.941 102 47 1 23 123 2 103 1.04e-31 114
MsG0180002270.01.T01 AT1G74590 50.926 108 52 1 25 131 7 114 1.25e-31 114
MsG0180002270.01.T01 AT1G78380 53.922 102 46 1 23 123 2 103 1.66e-31 113
MsG0180002270.01.T01 AT1G78320 52.475 101 47 1 24 123 3 103 5.25e-31 112
MsG0180002270.01.T01 AT1G17180 51.961 102 48 1 23 123 2 103 7.58e-31 111
MsG0180002270.01.T01 AT1G27130 48.673 113 54 3 21 129 2 114 2.30e-29 108
MsG0180002270.01.T01 AT1G78370 48.000 100 51 1 28 126 7 106 4.71e-29 107
MsG0180002270.01.T01 AT1G27140 48.649 111 52 3 21 126 2 112 1.73e-28 106
MsG0180002270.01.T01 AT1G53680 52.174 92 43 1 33 123 15 106 2.51e-28 105
MsG0180002270.01.T01 AT1G78360 51.456 103 48 2 23 123 2 104 4.12e-28 104
MsG0180002270.01.T01 AT1G17190 47.115 104 54 1 24 126 4 107 2.35e-27 102
MsG0180002270.01.T01 AT1G69920 47.170 106 53 2 21 123 30 135 7.52e-27 102
MsG0180002270.01.T01 AT3G43800 48.113 106 51 2 22 123 2 107 2.09e-26 100
MsG0180002270.01.T01 AT5G62480 38.776 98 35 1 25 122 8 80 1.07e-16 74.7
MsG0180002270.01.T01 AT5G62480 38.776 98 34 1 25 122 8 79 1.56e-16 74.3
MsG0180002270.01.T01 AT1G78320 38.000 100 36 1 24 123 3 76 3.74e-15 70.1

Find 34 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCTTTGGCTCGTTTC+TGG 0.167309 1:+36037770 MsG0180002270.01.T01:CDS
GTTCCTGTCTTTGTTCATAA+TGG 0.256856 1:+36037661 MsG0180002270.01.T01:CDS
TCAAATACAATCCAGTTTAC+AGG 0.271484 1:+36037635 MsG0180002270.01.T01:CDS
AAGAGCTTTGGCTCGTTTCT+GGG 0.368856 1:+36037771 MsG0180002270.01.T01:CDS
ACAGGAACCATCCTGTAAAC+TGG 0.379945 1:-36037646 None:intergenic
TAAGGATCATTCGGTAAGAT+TGG 0.380645 1:-36037745 None:intergenic
TTTCGCTACGGGAAGGTGTT+AGG 0.390074 1:+36037939 MsG0180002270.01.T01:CDS
TTGCAACACTCTCTTGACTT+TGG 0.438225 1:-36037458 None:intergenic
TACGTTTGAACCTTTCAAAC+CGG 0.442960 1:+36037846 MsG0180002270.01.T01:CDS
CGAGCCAAAGCTCTTTGATA+AGG 0.454417 1:-36037763 None:intergenic
TTTGAACTCTAATGACAAAC+GGG 0.454463 1:-36037540 None:intergenic
GCTCTTTGATAAGGATCATT+CGG 0.455855 1:-36037754 None:intergenic
TGATCCTTATCAAAGAGCTT+TGG 0.457027 1:+36037759 MsG0180002270.01.T01:CDS
AACCCTTGAGAATCGAGGTC+CGG 0.462024 1:+36037909 MsG0180002270.01.T01:CDS
ATTTGAACTCTAATGACAAA+CGG 0.463318 1:-36037541 None:intergenic
TCAAATTGCGCTGGAATTGA+AGG 0.463855 1:+36037558 MsG0180002270.01.T01:CDS
AAGAGAGTGTTGCAAGGCTA+TGG 0.471351 1:+36037466 MsG0180002270.01.T01:CDS
ACCCTTGAGAATCGAGGTCC+GGG 0.505758 1:+36037910 MsG0180002270.01.T01:CDS
TAAGATATCACCGGTTTGAA+AGG 0.506314 1:-36037856 None:intergenic
CATTAGAGTTCAAATTGCGC+TGG 0.507403 1:+36037549 MsG0180002270.01.T01:CDS
TTTCCATTATGAACAAAGAC+AGG 0.513163 1:-36037664 None:intergenic
TTGAGTACATCGATGAGACT+TGG 0.528066 1:+36037713 MsG0180002270.01.T01:CDS
CTCCCGGACCTCGATTCTCA+AGG 0.537806 1:-36037912 None:intergenic
AAAACAGTCATCAATGAACT+TGG 0.540012 1:-36037794 None:intergenic
CCCGTAGCGAAAACAACTCC+CGG 0.563638 1:-36037928 None:intergenic
GATTGCTGCATCTGCAGAAG+AGG 0.571501 1:+36037492 MsG0180002270.01.T01:CDS
CAAATTGCGCTGGAATTGAA+GGG 0.575623 1:+36037559 MsG0180002270.01.T01:CDS
ATCACACGAGACTCCGAGAT+GGG 0.589789 1:-36037688 None:intergenic
TCCCGGACCTCGATTCTCAA+GGG 0.595882 1:-36037911 None:intergenic
AATCACACGAGACTCCGAGA+TGG 0.605388 1:-36037689 None:intergenic
TCATAATGGAAATCCCATCT+CGG 0.646463 1:+36037675 MsG0180002270.01.T01:CDS
AGCAAAACCCTTGAGAATCG+AGG 0.652812 1:+36037904 MsG0180002270.01.T01:CDS
ATACAATCCAGTTTACAGGA+TGG 0.668642 1:+36037639 MsG0180002270.01.T01:CDS
AAAGTCAAGAGAGTGTTGCA+AGG 0.688685 1:+36037460 MsG0180002270.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTCCGAAGAAAAATT+GGG + Chr1:36037592-36037611 MsG0180002270.01.T01:CDS 20.0%
!! AAGAAAAAATAAGATATCAC+CGG - Chr1:36037868-36037887 None:intergenic 20.0%
!!! GTGATATCTTATTTTTTCTT+GGG + Chr1:36037868-36037887 MsG0180002270.01.T01:CDS 20.0%
! ATTTGAACTCTAATGACAAA+CGG - Chr1:36037544-36037563 None:intergenic 25.0%
! CAAATATTCCGAAGAAAAAT+TGG + Chr1:36037591-36037610 MsG0180002270.01.T01:CDS 25.0%
!!! ATCTTATTTTTTCTTGGGAA+GGG + Chr1:36037873-36037892 MsG0180002270.01.T01:CDS 25.0%
!!! GGTGATATCTTATTTTTTCT+TGG + Chr1:36037867-36037886 MsG0180002270.01.T01:CDS 25.0%
!!! TATCTTATTTTTTCTTGGGA+AGG + Chr1:36037872-36037891 MsG0180002270.01.T01:CDS 25.0%
AAAACAGTCATCAATGAACT+TGG - Chr1:36037797-36037816 None:intergenic 30.0%
TCAAATACAATCCAGTTTAC+AGG + Chr1:36037635-36037654 MsG0180002270.01.T01:CDS 30.0%
TTTCCATTATGAACAAAGAC+AGG - Chr1:36037667-36037686 None:intergenic 30.0%
TTTGAACTCTAATGACAAAC+GGG - Chr1:36037543-36037562 None:intergenic 30.0%
! CAAGTTACCCAATTTTTCTT+CGG - Chr1:36037602-36037621 None:intergenic 30.0%
!! ACTGTTTTATCTTGGTTTTC+GGG + Chr1:36037809-36037828 MsG0180002270.01.T01:CDS 30.0%
!! CATTGATGACTGTTTTATCT+TGG + Chr1:36037801-36037820 MsG0180002270.01.T01:CDS 30.0%
!! GACTGTTTTATCTTGGTTTT+CGG + Chr1:36037808-36037827 MsG0180002270.01.T01:CDS 30.0%
ATACAATCCAGTTTACAGGA+TGG + Chr1:36037639-36037658 MsG0180002270.01.T01:CDS 35.0%
GCTCTTTGATAAGGATCATT+CGG - Chr1:36037757-36037776 None:intergenic 35.0%
GTTCCTGTCTTTGTTCATAA+TGG + Chr1:36037661-36037680 MsG0180002270.01.T01:CDS 35.0%
TAAGGATCATTCGGTAAGAT+TGG - Chr1:36037748-36037767 None:intergenic 35.0%
TACGTTTGAACCTTTCAAAC+CGG + Chr1:36037846-36037865 MsG0180002270.01.T01:CDS 35.0%
TGATCCTTATCAAAGAGCTT+TGG + Chr1:36037759-36037778 MsG0180002270.01.T01:CDS 35.0%
! TCATAATGGAAATCCCATCT+CGG + Chr1:36037675-36037694 MsG0180002270.01.T01:CDS 35.0%
!! TAAGATATCACCGGTTTGAA+AGG - Chr1:36037859-36037878 None:intergenic 35.0%
CAAATTGCGCTGGAATTGAA+GGG + Chr1:36037559-36037578 MsG0180002270.01.T01:CDS 40.0%
CATTAGAGTTCAAATTGCGC+TGG + Chr1:36037549-36037568 MsG0180002270.01.T01:CDS 40.0%
TCAAATTGCGCTGGAATTGA+AGG + Chr1:36037558-36037577 MsG0180002270.01.T01:CDS 40.0%
TTGAGTACATCGATGAGACT+TGG + Chr1:36037713-36037732 MsG0180002270.01.T01:CDS 40.0%
TTGCAACACTCTCTTGACTT+TGG - Chr1:36037461-36037480 None:intergenic 40.0%
!! AAAGTCAAGAGAGTGTTGCA+AGG + Chr1:36037460-36037479 MsG0180002270.01.T01:CDS 40.0%
!!! GTGAAGCTTTTAGGTTCTGT+GGG + Chr1:36037514-36037533 MsG0180002270.01.T01:CDS 40.0%
ACAGGAACCATCCTGTAAAC+TGG - Chr1:36037649-36037668 None:intergenic 45.0%
AGCAAAACCCTTGAGAATCG+AGG + Chr1:36037904-36037923 MsG0180002270.01.T01:CDS 45.0%
CGAGCCAAAGCTCTTTGATA+AGG - Chr1:36037766-36037785 None:intergenic 45.0%
! AAAGAGCTTTGGCTCGTTTC+TGG + Chr1:36037770-36037789 MsG0180002270.01.T01:CDS 45.0%
! AAGAGCTTTGGCTCGTTTCT+GGG + Chr1:36037771-36037790 MsG0180002270.01.T01:CDS 45.0%
! GCAGAAGAGGTGAAGCTTTT+AGG + Chr1:36037505-36037524 MsG0180002270.01.T01:CDS 45.0%
!! AAGAGAGTGTTGCAAGGCTA+TGG + Chr1:36037466-36037485 MsG0180002270.01.T01:CDS 45.0%
!!! GGTGAAGCTTTTAGGTTCTG+TGG + Chr1:36037513-36037532 MsG0180002270.01.T01:CDS 45.0%
AACCCTTGAGAATCGAGGTC+CGG + Chr1:36037909-36037928 MsG0180002270.01.T01:CDS 50.0%
AATCACACGAGACTCCGAGA+TGG - Chr1:36037692-36037711 None:intergenic 50.0%
ATCACACGAGACTCCGAGAT+GGG - Chr1:36037691-36037710 None:intergenic 50.0%
GATTGCTGCATCTGCAGAAG+AGG + Chr1:36037492-36037511 MsG0180002270.01.T01:CDS 50.0%
!! GAGTTGTTTTCGCTACGGGA+AGG + Chr1:36037932-36037951 MsG0180002270.01.T01:CDS 50.0%
!! TCCGGGAGTTGTTTTCGCTA+CGG + Chr1:36037927-36037946 MsG0180002270.01.T01:CDS 50.0%
ACCCTTGAGAATCGAGGTCC+GGG + Chr1:36037910-36037929 MsG0180002270.01.T01:CDS 55.0%
CCCGTAGCGAAAACAACTCC+CGG - Chr1:36037931-36037950 None:intergenic 55.0%
TCCCGGACCTCGATTCTCAA+GGG - Chr1:36037914-36037933 None:intergenic 55.0%
!! CCGGGAGTTGTTTTCGCTAC+GGG + Chr1:36037928-36037947 MsG0180002270.01.T01:CDS 55.0%
CTCCCGGACCTCGATTCTCA+AGG - Chr1:36037915-36037934 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36037439 36037960 36037439 ID=MsG0180002270.01;Name=MsG0180002270.01
Chr1 mRNA 36037439 36037960 36037439 ID=MsG0180002270.01.T01;Parent=MsG0180002270.01;Name=MsG0180002270.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|173
Chr1 exon 36037439 36037960 36037439 ID=MsG0180002270.01.T01:exon:3281;Parent=MsG0180002270.01.T01
Chr1 CDS 36037439 36037960 36037439 ID=MsG0180002270.01.T01:cds;Parent=MsG0180002270.01.T01
Gene Sequence

>MsG0180002270.01.T01

ATGATTCATACATTAGTTGCCAAAGTCAAGAGAGTGTTGCAAGGCTATGGTTTGATTGCTGCATCTGCAGAAGAGGTGAAGCTTTTAGGTTCTGTGGGAAGCCCGTTTGTCATTAGAGTTCAAATTGCGCTGGAATTGAAGGGTATTGAATACAAATATTCCGAAGAAAAATTGGGTAACTTGAGTGAAACGCTTCTCAAATACAATCCAGTTTACAGGATGGTTCCTGTCTTTGTTCATAATGGAAATCCCATCTCGGAGTCTCGTGTGATTCTTGAGTACATCGATGAGACTTGGAAACAAAATCCAATCTTACCGAATGATCCTTATCAAAGAGCTTTGGCTCGTTTCTGGGCCAAGTTCATTGATGACTGTTTTATCTTGGTTTTCGGGTGTCAAACATTGATTACGTTTGAACCTTTCAAACCGGTGATATCTTATTTTTTCTTGGGAAGGGAAAAAGTAAGCAAAACCCTTGAGAATCGAGGTCCGGGAGTTGTTTTCGCTACGGGAAGGTGTTAG

Protein sequence

>MsG0180002270.01.T01

MIHTLVAKVKRVLQGYGLIAASAEEVKLLGSVGSPFVIRVQIALELKGIEYKYSEEKLGNLSETLLKYNPVYRMVPVFVHNGNPISESRVILEYIDETWKQNPILPNDPYQRALARFWAKFIDDCFILVFGCQTLITFEPFKPVISYFFLGREKVSKTLENRGPGVVFATGRC*