Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002452.01.T01 | XP_024631706.1 | 77.88 | 217 | 26 | 3 | 1 | 216 | 1 | 196 | 8.69E-81 | 263 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002452.01.T01 | Q05609 | 40.437 | 183 | 80 | 3 | 37 | 216 | 161 | 317 | 1.99E-26 | 109 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002452.01.T01 | A0A396JW60 | 77.880 | 217 | 26 | 3 | 1 | 216 | 1 | 196 | 6.89e-81 | 264 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002452.01 | MsG0180002453.01 | 0.814811 | 1.273869e-51 | 8.634074e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002452.01.T01 | MTR_1g050690 | 77.880 | 217 | 26 | 3 | 1 | 216 | 1 | 196 | 1.78e-84 | 264 |
| MsG0180002452.01.T01 | MTR_1g061190 | 43.784 | 185 | 80 | 3 | 32 | 216 | 128 | 288 | 5.92e-31 | 119 |
| MsG0180002452.01.T01 | MTR_5g066490 | 31.148 | 183 | 95 | 6 | 35 | 216 | 222 | 374 | 1.21e-11 | 63.9 |
| MsG0180002452.01.T01 | MTR_3g100090 | 35.644 | 101 | 64 | 1 | 116 | 216 | 238 | 337 | 1.87e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002452.01.T01 | AT5G03730 | 40.437 | 183 | 80 | 3 | 37 | 216 | 161 | 317 | 2.03e-27 | 109 |
| MsG0180002452.01.T01 | AT5G03730 | 40.437 | 183 | 80 | 3 | 37 | 216 | 161 | 317 | 2.03e-27 | 109 |
| MsG0180002452.01.T01 | AT4G24480 | 38.614 | 101 | 61 | 1 | 116 | 216 | 287 | 386 | 5.40e-12 | 65.1 |
| MsG0180002452.01.T01 | AT4G24480 | 38.614 | 101 | 61 | 1 | 116 | 216 | 287 | 386 | 5.77e-12 | 64.7 |
| MsG0180002452.01.T01 | AT4G24480 | 38.614 | 101 | 61 | 1 | 116 | 216 | 287 | 386 | 6.00e-12 | 64.7 |
Find 46 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGCTCAATCCATCGCTTCT+TGG | 0.164018 | 1:+38573193 | MsG0180002452.01.T01:CDS |
| AATCCATCGCTTCTTGGATT+TGG | 0.223297 | 1:+38573199 | MsG0180002452.01.T01:CDS |
| TATTAAGTCTTTCAAGTAAC+TGG | 0.270249 | 1:+38574146 | MsG0180002452.01.T01:CDS |
| CTTCTTCTTCTTTCAAAAGC+TGG | 0.270488 | 1:+38573099 | MsG0180002452.01.T01:CDS |
| CTGATCAGTTGCATCTTTGA+TGG | 0.334565 | 1:-38574173 | None:intergenic |
| ATGCATCCATCCCATGAATT+AGG | 0.339314 | 1:-38573970 | None:intergenic |
| TTTCACACTCACACCGGTTT+TGG | 0.343637 | 1:+38573275 | MsG0180002452.01.T01:CDS |
| GCCTTGAGAGAACTGCTAAC+TGG | 0.362157 | 1:+38574121 | MsG0180002452.01.T01:CDS |
| GTGAAAGTGAGTGAGGAATC+GGG | 0.397979 | 1:-38573258 | None:intergenic |
| TTCTTCTTCTTTCAAAAGCT+GGG | 0.403109 | 1:+38573100 | MsG0180002452.01.T01:CDS |
| TTGCAAATCTTGTTTGTAGT+AGG | 0.416842 | 1:+38574197 | MsG0180002452.01.T01:CDS |
| GACATTAGTGATTCAAATGA+TGG | 0.455196 | 1:-38574037 | None:intergenic |
| CCTTGAGAGAACTGCTAACT+GGG | 0.455830 | 1:+38574122 | MsG0180002452.01.T01:CDS |
| TGTGAAAGTGAGTGAGGAAT+CGG | 0.456489 | 1:-38573259 | None:intergenic |
| ATCTTTCCTGACCAGGTACA+TGG | 0.463838 | 1:+38573908 | MsG0180002452.01.T01:CDS |
| CATACCAACATTCTGCATAT+CGG | 0.467556 | 1:-38574011 | None:intergenic |
| AGGAGAATAAATACCAAAAC+CGG | 0.473930 | 1:-38573288 | None:intergenic |
| AGAAGAAGAAGGAGAAGGAG+AGG | 0.474237 | 1:-38573231 | None:intergenic |
| AAATCTTGTTTGTAGTAGGA+TGG | 0.484748 | 1:+38574201 | MsG0180002452.01.T01:CDS |
| TTCATTAATCTTTCCTGACC+AGG | 0.491053 | 1:+38573901 | MsG0180002452.01.T01:CDS |
| ATCATTTGAATCACTAATGT+CGG | 0.491978 | 1:+38574039 | MsG0180002452.01.T01:CDS |
| AAACAACCATGTACCTGGTC+AGG | 0.493856 | 1:-38573914 | None:intergenic |
| GTTAGCAGTTCTCTCAAGGC+AGG | 0.502796 | 1:-38574118 | None:intergenic |
| GAAGGAGAAGGAGAGGAGGT+AGG | 0.505965 | 1:-38573224 | None:intergenic |
| AGAAGAAGGAGAAGGAGAGG+AGG | 0.523827 | 1:-38573228 | None:intergenic |
| ATCTTGTTTGTAGTAGGATG+GGG | 0.546717 | 1:+38574203 | MsG0180002452.01.T01:CDS |
| AATCGGGAAGAAGAAGAAGA+AGG | 0.551468 | 1:-38573242 | None:intergenic |
| AATCTTGTTTGTAGTAGGAT+GGG | 0.552756 | 1:+38574202 | MsG0180002452.01.T01:CDS |
| CCCAGTTAGCAGTTCTCTCA+AGG | 0.560523 | 1:-38574122 | None:intergenic |
| GTTCCAAATCCAAGAAGCGA+TGG | 0.592557 | 1:-38573202 | None:intergenic |
| ATTGCAAACAACCATGTACC+TGG | 0.593108 | 1:-38573919 | None:intergenic |
| AGATAGGAAATGTACGGCGG+TGG | 0.596519 | 1:+38573002 | None:intergenic |
| GAAGATGAAGAAGACGAAGA+AGG | 0.596700 | 1:+38573074 | MsG0180002452.01.T01:CDS |
| AGTACCGATATGCAGAATGT+TGG | 0.601833 | 1:+38574007 | MsG0180002452.01.T01:CDS |
| TACTCTGACAAAGTTCTAGA+CGG | 0.602908 | 1:+38573941 | MsG0180002452.01.T01:CDS |
| CCAAGAAGCGATGGATTGAG+CGG | 0.604808 | 1:-38573193 | None:intergenic |
| ATGGGATGGATGCATATACA+TGG | 0.604853 | 1:+38573978 | MsG0180002452.01.T01:CDS |
| TGATAACTCGTCAGTTGTAG+TGG | 0.611571 | 1:+38574072 | MsG0180002452.01.T01:CDS |
| TGAAATGCGTGTGAAAAGAG+AGG | 0.623301 | 1:-38573308 | None:intergenic |
| ACTCACTTTCACACTCACAC+CGG | 0.624967 | 1:+38573269 | MsG0180002452.01.T01:CDS |
| TTAGCAGTTCTCTCAAGGCA+GGG | 0.628638 | 1:-38574117 | None:intergenic |
| GAGCTGAGATAGGAAATGTA+CGG | 0.628659 | 1:+38572996 | None:intergenic |
| ACAACTGACGAGTTATCACA+CGG | 0.704651 | 1:-38574067 | None:intergenic |
| TGTGAGTGTGAAAGTGAGTG+AGG | 0.712861 | 1:-38573265 | None:intergenic |
| CTTGAGAGAACTGCTAACTG+GGG | 0.732889 | 1:+38574123 | MsG0180002452.01.T01:CDS |
| CTGAGATAGGAAATGTACGG+CGG | 0.744607 | 1:+38572999 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCTTATATTAATAAACTATT+TGG | - | Chr1:38573470-38573489 | None:intergenic | 15.0% |
| !!! | ATTAATAAACTATTTGGTTT+TGG | - | Chr1:38573464-38573483 | None:intergenic | 15.0% |
| !! | ATGAACAAAACTTGTATATA+TGG | - | Chr1:38573849-38573868 | None:intergenic | 20.0% |
| !! | TAAATTACATAAGCACTTAT+AGG | - | Chr1:38573413-38573432 | None:intergenic | 20.0% |
| !!! | ATATTCAATTAAAAGGTACA+TGG | - | Chr1:38573803-38573822 | None:intergenic | 20.0% |
| !!! | TTGTACTTGTAAGAAATTTT+AGG | - | Chr1:38573623-38573642 | None:intergenic | 20.0% |
| ! | ACTTATTTGAGCTTACTATT+TGG | - | Chr1:38573586-38573605 | None:intergenic | 25.0% |
| ! | ATCATTTGAATCACTAATGT+CGG | + | Chr1:38574039-38574058 | MsG0180002452.01.T01:CDS | 25.0% |
| ! | CGACTGAATATTCAATTAAA+AGG | - | Chr1:38573810-38573829 | None:intergenic | 25.0% |
| ! | TAACAAAACTCGATTTACTT+TGG | + | Chr1:38573362-38573381 | MsG0180002452.01.T01:intron | 25.0% |
| ! | TTAATTGAATATTCAGTCGA+TGG | + | Chr1:38573811-38573830 | MsG0180002452.01.T01:intron | 25.0% |
| !! | TATTAAGTCTTTCAAGTAAC+TGG | + | Chr1:38574146-38574165 | MsG0180002452.01.T01:CDS | 25.0% |
| AGGAGAATAAATACCAAAAC+CGG | - | Chr1:38573291-38573310 | None:intergenic | 30.0% | |
| CAAAGCTAATTTACACTATC+AGG | - | Chr1:38573773-38573792 | None:intergenic | 30.0% | |
| CTTACTATTTGGATAGCTTA+TGG | - | Chr1:38573575-38573594 | None:intergenic | 30.0% | |
| GACATTAGTGATTCAAATGA+TGG | - | Chr1:38574040-38574059 | None:intergenic | 30.0% | |
| TCAAATAAGTCAATCCATAC+AGG | + | Chr1:38573596-38573615 | MsG0180002452.01.T01:intron | 30.0% | |
| TTCTTCTTCTTTCAAAAGCT+GGG | + | Chr1:38573100-38573119 | MsG0180002452.01.T01:CDS | 30.0% | |
| ! | AAATCTTGTTTGTAGTAGGA+TGG | + | Chr1:38574201-38574220 | MsG0180002452.01.T01:CDS | 30.0% |
| ! | AATCTTGTTTGTAGTAGGAT+GGG | + | Chr1:38574202-38574221 | MsG0180002452.01.T01:CDS | 30.0% |
| ! | ACGGATTTTACCTAATTCAT+GGG | + | Chr1:38573960-38573979 | MsG0180002452.01.T01:CDS | 30.0% |
| ! | ATTTTACCTAATTCATGGGA+TGG | + | Chr1:38573964-38573983 | MsG0180002452.01.T01:CDS | 30.0% |
| ! | CTGATAGTGTAAATTAGCTT+TGG | + | Chr1:38573771-38573790 | MsG0180002452.01.T01:intron | 30.0% |
| ! | TTGCAAATCTTGTTTGTAGT+AGG | + | Chr1:38574197-38574216 | MsG0180002452.01.T01:CDS | 30.0% |
| !!! | AAGAAATTTTAGGACCTGTA+TGG | - | Chr1:38573613-38573632 | None:intergenic | 30.0% |
| !!! | AGTTTTGTTATTGCGATGAT+AGG | - | Chr1:38573352-38573371 | None:intergenic | 30.0% |
| AATAAGCTGAAAACAGCTCA+TGG | + | Chr1:38573531-38573550 | MsG0180002452.01.T01:intron | 35.0% | |
| CATACCAACATTCTGCATAT+CGG | - | Chr1:38574014-38574033 | None:intergenic | 35.0% | |
| CTTCTTCTTCTTTCAAAAGC+TGG | + | Chr1:38573099-38573118 | MsG0180002452.01.T01:CDS | 35.0% | |
| TGAACACCTGATATAATTCC+AGG | - | Chr1:38573885-38573904 | None:intergenic | 35.0% | |
| TTCATTAATCTTTCCTGACC+AGG | + | Chr1:38573864-38573883 | MsG0180002452.01.T01:intron | 35.0% | |
| TTCATTAATCTTTCCTGACC+TGG | + | Chr1:38573864-38573883 | MsG0180002452.01.T01:intron | 35.0% | |
| ! | ATCTTGTTTGTAGTAGGATG+GGG | + | Chr1:38574203-38574222 | MsG0180002452.01.T01:CDS | 35.0% |
| !! | GACGGATTTTACCTAATTCA+TGG | + | Chr1:38573959-38573978 | MsG0180002452.01.T01:CDS | 35.0% |
| !! | TACTCTGACAAAGTTCTAGA+CGG | + | Chr1:38573941-38573960 | MsG0180002452.01.T01:CDS | 35.0% |
| AATCCATCGCTTCTTGGATT+TGG | + | Chr1:38573199-38573218 | MsG0180002452.01.T01:CDS | 40.0% | |
| AATCGGGAAGAAGAAGAAGA+AGG | - | Chr1:38573245-38573264 | None:intergenic | 40.0% | |
| ACAACTGACGAGTTATCACA+CGG | - | Chr1:38574070-38574089 | None:intergenic | 40.0% | |
| ACAAGATACAAACCACGTAC+TGG | + | Chr1:38573650-38573669 | MsG0180002452.01.T01:intron | 40.0% | |
| ACCTGATATAATTCCAGGTC+AGG | - | Chr1:38573880-38573899 | None:intergenic | 40.0% | |
| ATGCATCCATCCCATGAATT+AGG | - | Chr1:38573973-38573992 | None:intergenic | 40.0% | |
| ATGGGATGGATGCATATACA+TGG | + | Chr1:38573978-38573997 | MsG0180002452.01.T01:CDS | 40.0% | |
| ATTGCAAACAACCATGTACC+TGG | - | Chr1:38573922-38573941 | None:intergenic | 40.0% | |
| CTGATCAGTTGCATCTTTGA+TGG | - | Chr1:38574176-38574195 | None:intergenic | 40.0% | |
| GAAGAAGAAGAAGAAGGAGA+AGG | - | Chr1:38573239-38573258 | None:intergenic | 40.0% | |
| GAAGATGAAGAAGACGAAGA+AGG | + | Chr1:38573074-38573093 | MsG0180002452.01.T01:CDS | 40.0% | |
| TCCTGACCTGGAATTATATC+AGG | + | Chr1:38573876-38573895 | MsG0180002452.01.T01:intron | 40.0% | |
| TGAAATGCGTGTGAAAAGAG+AGG | - | Chr1:38573311-38573330 | None:intergenic | 40.0% | |
| TGATAACTCGTCAGTTGTAG+TGG | + | Chr1:38574072-38574091 | MsG0180002452.01.T01:CDS | 40.0% | |
| TGTGAAAGTGAGTGAGGAAT+CGG | - | Chr1:38573262-38573281 | None:intergenic | 40.0% | |
| ! | AGCTAGCTTGAAGAATCTTC+AGG | + | Chr1:38573507-38573526 | MsG0180002452.01.T01:intron | 40.0% |
| !! | AGTACCGATATGCAGAATGT+TGG | + | Chr1:38574007-38574026 | MsG0180002452.01.T01:CDS | 40.0% |
| AAACAACCATGTACCTGGTC+AGG | - | Chr1:38573917-38573936 | None:intergenic | 45.0% | |
| AAGTTAGCACAACCAGTACG+TGG | - | Chr1:38573665-38573684 | None:intergenic | 45.0% | |
| ACTCACTTTCACACTCACAC+CGG | + | Chr1:38573269-38573288 | MsG0180002452.01.T01:CDS | 45.0% | |
| AGAAGAAGAAGGAGAAGGAG+AGG | - | Chr1:38573234-38573253 | None:intergenic | 45.0% | |
| ATCTTTCCTGACCAGGTACA+TGG | + | Chr1:38573908-38573927 | MsG0180002452.01.T01:CDS | 45.0% | |
| CCTTGAGAGAACTGCTAACT+GGG | + | Chr1:38574122-38574141 | MsG0180002452.01.T01:CDS | 45.0% | |
| CTTGAGAGAACTGCTAACTG+GGG | + | Chr1:38574123-38574142 | MsG0180002452.01.T01:CDS | 45.0% | |
| GTGAAAGTGAGTGAGGAATC+GGG | - | Chr1:38573261-38573280 | None:intergenic | 45.0% | |
| GTTCCAAATCCAAGAAGCGA+TGG | - | Chr1:38573205-38573224 | None:intergenic | 45.0% | |
| TGTGAGTGTGAAAGTGAGTG+AGG | - | Chr1:38573268-38573287 | None:intergenic | 45.0% | |
| TTAGCAGTTCTCTCAAGGCA+GGG | - | Chr1:38574120-38574139 | None:intergenic | 45.0% | |
| !!! | TTTCACACTCACACCGGTTT+TGG | + | Chr1:38573275-38573294 | MsG0180002452.01.T01:CDS | 45.0% |
| AGAAGAAGGAGAAGGAGAGG+AGG | - | Chr1:38573231-38573250 | None:intergenic | 50.0% | |
| CCCAGTTAGCAGTTCTCTCA+AGG | - | Chr1:38574125-38574144 | None:intergenic | 50.0% | |
| GCCTTGAGAGAACTGCTAAC+TGG | + | Chr1:38574121-38574140 | MsG0180002452.01.T01:CDS | 50.0% | |
| GTTAGCAGTTCTCTCAAGGC+AGG | - | Chr1:38574121-38574140 | None:intergenic | 50.0% | |
| !! | CCAAGAAGCGATGGATTGAG+CGG | - | Chr1:38573196-38573215 | None:intergenic | 50.0% |
| CCGCTCAATCCATCGCTTCT+TGG | + | Chr1:38573193-38573212 | MsG0180002452.01.T01:CDS | 55.0% | |
| GAAGGAGAAGGAGAGGAGGT+AGG | - | Chr1:38573227-38573246 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 38573011 | 38574228 | 38573011 | ID=MsG0180002452.01;Name=MsG0180002452.01 |
| Chr1 | mRNA | 38573011 | 38574228 | 38573011 | ID=MsG0180002452.01.T01;Parent=MsG0180002452.01;Name=MsG0180002452.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|0|0.5|2|0|216 |
| Chr1 | exon | 38573011 | 38573330 | 38573011 | ID=MsG0180002452.01.T01:exon:12763;Parent=MsG0180002452.01.T01 |
| Chr1 | exon | 38573898 | 38574228 | 38573898 | ID=MsG0180002452.01.T01:exon:12762;Parent=MsG0180002452.01.T01 |
| Chr1 | CDS | 38573011 | 38573330 | 38573011 | ID=MsG0180002452.01.T01:cds;Parent=MsG0180002452.01.T01 |
| Chr1 | CDS | 38573898 | 38574228 | 38573898 | ID=MsG0180002452.01.T01:cds;Parent=MsG0180002452.01.T01 |
| Gene Sequence |
| Protein sequence |
