AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003462.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003462.01.T01 MTR_1g062590 88.750 240 24 1 17 253 3 242 3.83e-156 434
MsG0180003462.01.T01 MTR_1g062660 85.463 227 33 0 27 253 16 242 4.62e-143 400
MsG0180003462.01.T01 MTR_1g062370 80.165 242 48 0 12 253 1 242 1.08e-141 397
MsG0180003462.01.T01 MTR_1g062350 80.165 242 48 0 12 253 1 242 1.51e-141 397
MsG0180003462.01.T01 MTR_1g062630 84.581 227 35 0 27 253 16 242 2.65e-141 397
MsG0180003462.01.T01 MTR_1g062340 82.379 227 40 0 27 253 16 242 5.36e-139 390
MsG0180003462.01.T01 MTR_7g102380 72.532 233 63 1 21 253 10 241 3.90e-125 355
MsG0180003462.01.T01 MTR_1g062690 70.661 242 47 1 12 253 1 218 2.10e-118 337
MsG0180003462.01.T01 MTR_2g069660 63.559 236 83 2 21 253 8 243 1.24e-102 298
MsG0180003462.01.T01 MTR_1g025420 55.102 245 106 4 12 252 1 245 3.83e-89 265
MsG0180003462.01.T01 MTR_1g025420 55.102 245 106 4 12 252 1 245 4.10e-89 267
MsG0180003462.01.T01 MTR_3g114030 56.502 223 94 3 34 253 8 230 4.34e-84 251
MsG0180003462.01.T01 MTR_8g075550 54.626 227 95 3 33 252 28 253 2.46e-82 249
MsG0180003462.01.T01 MTR_4g073720 53.719 242 105 5 12 252 4 239 3.70e-82 250
MsG0180003462.01.T01 MTR_5g022350 54.667 225 92 6 30 253 28 243 3.45e-80 244
MsG0180003462.01.T01 MTR_8g037890 50.424 236 112 4 21 253 14 247 4.88e-78 238
MsG0180003462.01.T01 MTR_8g056820 50.424 236 112 4 21 253 14 247 1.01e-77 236
MsG0180003462.01.T01 MTR_8g075510 46.245 253 120 6 2 253 7 244 1.21e-77 235
MsG0180003462.01.T01 MTR_1g021945 57.333 225 88 7 33 253 25 245 5.92e-77 233
MsG0180003462.01.T01 MTR_8g075510 46.032 252 120 6 2 252 7 243 1.49e-76 234
MsG0180003462.01.T01 MTR_4g073730 49.789 237 114 4 21 253 13 248 4.62e-76 233
MsG0180003462.01.T01 MTR_5g022310 48.780 246 117 5 16 253 7 251 5.67e-76 231
MsG0180003462.01.T01 MTR_5g022310 48.780 246 117 5 16 253 7 251 7.65e-76 232
MsG0180003462.01.T01 MTR_4g073730 49.789 237 114 4 21 253 13 248 1.03e-75 233
MsG0180003462.01.T01 MTR_8g036215 48.101 237 117 5 19 252 4 237 1.10e-74 227
MsG0180003462.01.T01 MTR_3g111620 53.125 224 100 5 32 253 26 246 1.76e-72 222
MsG0180003462.01.T01 MTR_8g107140 48.936 235 114 5 21 253 15 245 5.45e-69 213
MsG0180003462.01.T01 MTR_6g079580 45.957 235 121 5 21 253 10 240 7.08e-69 212
MsG0180003462.01.T01 MTR_2g067980 45.565 248 126 5 11 253 4 247 1.09e-68 228
MsG0180003462.01.T01 MTR_7g062610 46.383 235 121 5 21 253 11 242 2.96e-68 211
MsG0180003462.01.T01 MTR_2g068030 46.364 220 113 4 36 252 26 243 6.05e-67 208
MsG0180003462.01.T01 MTR_3g068015 44.889 225 118 5 32 253 27 248 4.46e-66 206
MsG0180003462.01.T01 MTR_2g068655 46.429 224 115 4 33 253 31 252 4.29e-65 205
MsG0180003462.01.T01 MTR_4g063630 44.828 232 120 6 27 252 22 251 6.54e-63 197
MsG0180003462.01.T01 MTR_6g009480 44.248 226 119 5 33 253 29 252 7.09e-61 193
MsG0180003462.01.T01 MTR_5g059200 47.248 218 104 7 36 252 32 239 1.38e-59 190
MsG0180003462.01.T01 MTR_7g076360 43.128 211 56 4 33 242 8 155 1.36e-44 148
MsG0180003462.01.T01 MTR_5g022350 55.639 133 54 3 30 161 93 221 1.67e-44 149
MsG0180003462.01.T01 MTR_5g023850 39.301 229 118 9 12 229 2 220 3.28e-44 149
MsG0180003462.01.T01 MTR_5g010635 39.910 223 111 8 33 253 24 225 7.91e-44 148
MsG0180003462.01.T01 MTR_5g010640 36.929 241 126 8 21 253 14 236 8.19e-44 148
MsG0180003462.01.T01 MTR_2g067970 42.935 184 95 7 76 253 3 182 1.77e-42 154
MsG0180003462.01.T01 MTR_8g096900 38.819 237 116 10 21 253 37 248 2.23e-42 145
MsG0180003462.01.T01 MTR_2g067990 39.381 226 108 9 30 253 24 222 7.98e-42 152
MsG0180003462.01.T01 MTR_1g062640 72.340 94 26 0 79 172 53 146 3.00e-40 136
MsG0180003462.01.T01 MTR_8g089020 53.175 126 55 2 117 242 33 154 2.28e-39 134
MsG0180003462.01.T01 MTR_2g063150 34.579 214 133 5 30 241 31 239 2.28e-38 135
MsG0180003462.01.T01 MTR_1g062390 32.365 241 139 7 21 253 14 238 6.87e-34 122
MsG0180003462.01.T01 MTR_8g096920 36.538 156 83 7 15 164 2 147 3.33e-18 79.7
MsG0180003462.01.T01 MTR_3g081550 48.315 89 44 2 117 203 16 104 6.83e-18 77.4
MsG0180003462.01.T01 MTR_8g096910 35.417 144 78 6 27 164 16 150 7.19e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003462.01.T01 AT1G20030 55.556 225 95 3 34 253 4 228 3.06e-86 259
MsG0180003462.01.T01 AT1G20030 55.022 229 98 3 30 253 17 245 3.46e-86 259
MsG0180003462.01.T01 AT1G19320 54.618 249 99 7 14 252 2 246 4.12e-84 251
MsG0180003462.01.T01 AT1G75800 51.667 240 109 4 21 253 9 248 5.00e-84 254
MsG0180003462.01.T01 AT4G38660 55.556 234 94 5 21 253 21 245 8.45e-84 254
MsG0180003462.01.T01 AT4G38660 56.502 223 90 4 32 253 7 223 1.75e-83 253
MsG0180003462.01.T01 AT1G75040 54.661 236 96 7 21 253 12 239 1.49e-81 244
MsG0180003462.01.T01 AT2G17860 50.598 251 113 6 12 253 1 249 3.36e-80 242
MsG0180003462.01.T01 AT4G24180 51.613 248 105 6 16 253 14 256 1.41e-79 240
MsG0180003462.01.T01 AT4G24180 51.807 249 105 6 15 253 8 251 2.21e-79 239
MsG0180003462.01.T01 AT4G24180 51.807 249 105 6 15 253 8 251 7.68e-79 240
MsG0180003462.01.T01 AT4G24180 51.613 248 105 6 16 253 14 256 7.86e-79 240
MsG0180003462.01.T01 AT1G75050 52.439 246 107 8 14 253 2 243 1.06e-77 235
MsG0180003462.01.T01 AT4G36010 50.855 234 106 3 28 253 18 250 7.65e-77 235
MsG0180003462.01.T01 AT4G36010 50.855 234 106 3 28 253 18 250 7.65e-77 235
MsG0180003462.01.T01 AT4G38670 47.967 246 124 3 12 253 2 247 1.00e-76 234
MsG0180003462.01.T01 AT4G38670 47.967 246 124 3 12 253 2 247 1.00e-76 234
MsG0180003462.01.T01 AT1G75030 56.000 225 91 8 33 253 23 243 3.11e-76 231
MsG0180003462.01.T01 AT4G38670 51.339 224 105 3 34 253 8 231 5.96e-76 231
MsG0180003462.01.T01 AT5G38280 49.167 240 115 5 21 253 11 250 3.98e-74 238
MsG0180003462.01.T01 AT5G38280 49.167 240 115 5 21 253 11 250 5.26e-74 238
MsG0180003462.01.T01 AT1G73620 50.673 223 104 5 33 253 42 260 3.51e-72 222
MsG0180003462.01.T01 AT4G24180 50.431 232 101 5 16 237 14 241 1.04e-71 223
MsG0180003462.01.T01 AT4G24180 50.644 233 101 5 15 237 8 236 1.13e-71 222
MsG0180003462.01.T01 AT1G18250 49.123 228 110 5 28 253 17 240 2.51e-70 216
MsG0180003462.01.T01 AT1G18250 50.224 223 105 5 33 253 21 239 2.59e-70 216
MsG0180003462.01.T01 AT5G24620 50.000 242 110 7 21 253 12 251 2.22e-68 217
MsG0180003462.01.T01 AT5G24620 50.000 242 110 7 21 253 67 306 4.88e-68 218
MsG0180003462.01.T01 AT5G24620 50.000 242 110 7 21 253 88 327 5.68e-68 218
MsG0180003462.01.T01 AT5G02140 45.532 235 124 4 21 253 9 241 3.64e-64 201
MsG0180003462.01.T01 AT2G28790 40.816 245 139 4 11 252 7 248 5.03e-64 200
MsG0180003462.01.T01 AT5G02140 45.532 235 124 4 21 253 9 241 5.76e-64 202
MsG0180003462.01.T01 AT2G28790 40.816 245 139 4 11 252 32 273 5.85e-64 201
MsG0180003462.01.T01 AT5G40020 43.231 229 124 5 29 253 22 248 1.57e-60 192
MsG0180003462.01.T01 AT1G77700 43.580 257 115 9 16 252 53 299 2.49e-60 194
MsG0180003462.01.T01 AT1G77700 46.018 226 111 7 28 252 56 271 5.82e-60 192
MsG0180003462.01.T01 AT1G77700 46.018 226 111 7 28 252 56 271 6.19e-60 192
MsG0180003462.01.T01 AT4G18253 39.241 237 133 4 21 253 9 238 1.60e-52 171
MsG0180003462.01.T01 AT1G70250 42.731 227 114 8 36 253 148 367 1.92e-50 176
MsG0180003462.01.T01 AT4G18250 40.506 237 131 6 21 253 42 272 2.77e-49 172
MsG0180003462.01.T01 AT4G36000 52.740 146 61 3 108 253 1 138 1.13e-45 152
MsG0180003462.01.T01 AT4G36000 52.740 146 61 3 108 253 1 138 1.13e-45 152
MsG0180003462.01.T01 AT4G11650 39.914 233 116 9 23 253 13 223 4.25e-42 144
MsG0180003462.01.T01 AT5G24620 54.167 120 48 3 140 253 16 134 2.13e-33 123
MsG0180003462.01.T01 AT2G24810 58.228 79 31 2 174 252 116 192 2.69e-23 94.0

Find 83 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATGGTCAGGTCGATTC+TGG 0.113933 1:-62642117 MsG0180003462.01.T01:CDS
ATAGAAGGTATAGTTCAATT+AGG 0.247754 1:+62641589 None:intergenic
CCCGCAGAGCTTCAATTAAA+AGG 0.268083 1:-62641833 MsG0180003462.01.T01:CDS
TGGTTTACATACAATAAATA+TGG 0.268242 1:+62642671 None:intergenic
TGATCTCCAGTTAAGGTTCC+TGG 0.276179 1:+62642208 None:intergenic
CCCATGGTCAGGTCGATTCT+GGG 0.333468 1:-62642116 MsG0180003462.01.T01:CDS
TAGGCATAGCTATAAGCTTC+TGG 0.336607 1:+62641674 None:intergenic
CGCAATCCCACCAGCAACTT+TGG 0.341693 1:-62642005 MsG0180003462.01.T01:CDS
GTATGGCCAGGAACCTTAAC+TGG 0.347390 1:-62642214 MsG0180003462.01.T01:CDS
AGCACTTTCACTTGCTCTAA+TGG 0.354302 1:-62641638 MsG0180003462.01.T01:CDS
TAGAAGGTATAGTTCAATTA+GGG 0.357199 1:+62641590 None:intergenic
CACACAGAGTTGATATTTGC+TGG 0.361358 1:+62641857 None:intergenic
GTTATTTGAGCATCCGGTTC+GGG 0.368660 1:+62642093 None:intergenic
GCCACAGCCGATTGCGCGTC+TGG 0.381507 1:-62642052 MsG0180003462.01.T01:CDS
TCCGTTGTTATTTGAGCATC+CGG 0.387240 1:+62642087 None:intergenic
CTCTGTGTTTAAAATAAAAC+AGG 0.404086 1:-62641553 MsG0180003462.01.T01:three_prime_UTR
TGCTTGTAAGAGTGCTTGTT+TGG 0.407476 1:-62641792 MsG0180003462.01.T01:CDS
TGTTATTTGAGCATCCGGTT+CGG 0.415751 1:+62642092 None:intergenic
GTAATTTCAACCAAAGTTGC+TGG 0.422022 1:+62641995 None:intergenic
TGATATTTCATACTTATTTG+TGG 0.431329 1:+62641491 None:intergenic
AGACATAACTGTATTATAGA+AGG 0.453184 1:+62641574 None:intergenic
AACTTGACCAGACGCGCAAT+CGG 0.454519 1:+62642045 None:intergenic
GAAATTACCGTGGCATCAAA+TGG 0.464138 1:-62641980 MsG0180003462.01.T01:CDS
GACCTTCCTTCCCCATGGTC+AGG 0.467806 1:-62642127 MsG0180003462.01.T01:CDS
AAATATCTCGGAGTAATTTG+TGG 0.469027 1:+62641709 None:intergenic
AGAGCTTCAATTAAAAGGTT+CGG 0.473590 1:-62641828 MsG0180003462.01.T01:CDS
ATCCAATGGCCACAATTCAT+TGG 0.473986 1:+62642729 None:intergenic
GGTCGATTCTGGGCCCGAAC+CGG 0.475671 1:-62642106 MsG0180003462.01.T01:CDS
GTGACTCCACAAGGCGGGAG+TGG 0.497014 1:-62641902 MsG0180003462.01.T01:CDS
AAGCCTCAACTATCAACAAC+AGG 0.497185 1:-62642184 MsG0180003462.01.T01:CDS
CCCAGAATCGACCTGACCAT+GGG 0.498071 1:+62642116 None:intergenic
CAAGTTGACTGCAATGGTGC+TGG 0.502641 1:-62642028 MsG0180003462.01.T01:CDS
ACCAGACGCGCAATCGGCTG+TGG 0.503521 1:+62642051 None:intergenic
AATATCTCGGAGTAATTTGT+GGG 0.505487 1:+62641710 None:intergenic
AATGGCCACAATTCATTGGT+GGG 0.507125 1:+62642733 None:intergenic
CACCAATGAATTGTGGCCAT+TGG 0.507193 1:-62642731 MsG0180003462.01.T01:CDS
AAACCTGTTGTTGATAGTTG+AGG 0.508027 1:+62642181 None:intergenic
CTTCAATTAAAAGGTTCGGA+TGG 0.512580 1:-62641824 MsG0180003462.01.T01:CDS
AGCTATAAGCTTCTGGACAT+TGG 0.513068 1:+62641681 None:intergenic
CCTTGTGGAGTCACGGACAT+TGG 0.533550 1:+62641911 None:intergenic
CAATCGCCACTCCCGCCTTG+TGG 0.533640 1:+62641896 None:intergenic
GGTTTACATACAATAAATAT+GGG 0.536439 1:+62642672 None:intergenic
CAGAATGTGATGGCATAGCT+AGG 0.537085 1:+62641614 None:intergenic
TTCAATTAAAAGGTTCGGAT+GGG 0.546771 1:-62641823 MsG0180003462.01.T01:CDS
TTGTCCTCCATTTGATGCCA+CGG 0.548889 1:+62641973 None:intergenic
GCCCAGAATCGACCTGACCA+TGG 0.560288 1:+62642115 None:intergenic
TGTCCGTGACTCCACAAGGC+GGG 0.562371 1:-62641907 MsG0180003462.01.T01:CDS
ATAGATGTGGATACACTGTA+TGG 0.562425 1:-62642231 MsG0180003462.01.T01:CDS
ACCGATCAATATTGTTGTCG+TGG 0.565802 1:-62641761 MsG0180003462.01.T01:CDS
CAATGGCCACAATTCATTGG+TGG 0.566116 1:+62642732 None:intergenic
TGTGGATACACTGTATGGCC+AGG 0.574199 1:-62642226 MsG0180003462.01.T01:CDS
TACGATGTGAGCAACGTGGA+CGG 0.575852 1:-62641944 MsG0180003462.01.T01:CDS
GATATTTGCTGGACAACTAG+AGG 0.578884 1:+62641868 None:intergenic
CCAATGTCCGTGACTCCACA+AGG 0.583086 1:-62641911 MsG0180003462.01.T01:CDS
ATTTCAACCAAAGTTGCTGG+TGG 0.585079 1:+62641998 None:intergenic
GACCTGACCATGGGGAAGGA+AGG 0.587448 1:+62642125 None:intergenic
TGTGTTTAAAATAAAACAGG+TGG 0.590116 1:-62641550 MsG0180003462.01.T01:three_prime_UTR
ACATTGGTTCTCAAATATCT+CGG 0.592729 1:+62641697 None:intergenic
TTTCAACCAAAGTTGCTGGT+GGG 0.596283 1:+62641999 None:intergenic
AGTGCTGCTCTTATCATCGT+AGG 0.606860 1:+62641655 None:intergenic
AATCGACCTGACCATGGGGA+AGG 0.607599 1:+62642121 None:intergenic
ACAACCCCACCAATGAATTG+TGG 0.618276 1:-62642738 MsG0180003462.01.T01:CDS
TCCACGACAACAATATTGAT+CGG 0.620824 1:+62641760 None:intergenic
GTGACATTCACAAATAGATG+TGG 0.620825 1:-62642244 MsG0180003462.01.T01:CDS
GCTACATATGCTGCATCCAA+TGG 0.620950 1:+62642715 None:intergenic
CAGTGGACCTTCCTTCCCCA+TGG 0.623305 1:-62642132 MsG0180003462.01.T01:CDS
ATCTCGGAGTAATTTGTGGG+TGG 0.627398 1:+62641713 None:intergenic
TCTGGTCAAGTTGACTGCAA+TGG 0.629280 1:-62642034 MsG0180003462.01.T01:CDS
ACAAGGAGCATCAAGATCAG+TGG 0.632227 1:-62642149 MsG0180003462.01.T01:CDS
ACTCCCGCCTTGTGGAGTCA+CGG 0.634641 1:+62641904 None:intergenic
AACTTTGGTTGAAATTACCG+TGG 0.634789 1:-62641990 MsG0180003462.01.T01:CDS
GATGCTCAAATAACAACGGA+AGG 0.637443 1:-62642084 MsG0180003462.01.T01:CDS
TGTGGGTGGACATGTCGCCT+CGG 0.637786 1:+62641727 None:intergenic
ATGGCCACAATTCATTGGTG+GGG 0.639497 1:+62642734 None:intergenic
CAATTAGGGACAGAATGTGA+TGG 0.639803 1:+62641604 None:intergenic
TGCAGCTGTTGGAGGAGCCA+CGG 0.641900 1:-62642266 MsG0180003462.01.T01:intron
ATTACCGTGGCATCAAATGG+AGG 0.665292 1:-62641977 MsG0180003462.01.T01:CDS
ATGTCCGTGACTCCACAAGG+CGG 0.680695 1:-62641908 MsG0180003462.01.T01:CDS
CCAGAATCGACCTGACCATG+GGG 0.688852 1:+62642117 None:intergenic
ACCGGATGCTCAAATAACAA+CGG 0.699128 1:-62642088 MsG0180003462.01.T01:CDS
TTTCTACGATGTGAGCAACG+TGG 0.737287 1:-62641948 MsG0180003462.01.T01:CDS
TCTATTTGTGAATGTCACCG+TGG 0.760029 1:+62642249 None:intergenic
TCGTGGAAGTTACAACACCG+AGG 0.789168 1:-62641744 MsG0180003462.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTATTGTTATTTTTTTTA+AGG - Chr1:62642730-62642749 MsG0180003462.01.T01:CDS 10.0%
!!! TTATTTTTAACCATATGAAA+AGG + Chr1:62641549-62641568 None:intergenic 15.0%
!! GGTTTACATACAATAAATAT+GGG + Chr1:62641483-62641502 None:intergenic 20.0%
!! TGATATTTCATACTTATTTG+TGG + Chr1:62642664-62642683 None:intergenic 20.0%
!! TGGTTTACATACAATAAATA+TGG + Chr1:62641484-62641503 None:intergenic 20.0%
!!! TTATCACATTTTTTTCTTGT+AGG - Chr1:62641706-62641725 MsG0180003462.01.T01:CDS 20.0%
! AAAGATAACGAGTATAATCT+AGG + Chr1:62641654-62641673 None:intergenic 25.0%
! AAGATAACGAGTATAATCTA+GGG + Chr1:62641653-62641672 None:intergenic 25.0%
! AGACATAACTGTATTATAGA+AGG + Chr1:62642581-62642600 None:intergenic 25.0%
! TGTAGGTATCTAAATACTAT+CGG - Chr1:62641723-62641742 MsG0180003462.01.T01:CDS 25.0%
!! ATAGAAGGTATAGTTCAATT+AGG + Chr1:62642566-62642585 None:intergenic 25.0%
!! CTCTGTGTTTAAAATAAAAC+AGG - Chr1:62642599-62642618 MsG0180003462.01.T01:intron 25.0%
!! TAGAAGGTATAGTTCAATTA+GGG + Chr1:62642565-62642584 None:intergenic 25.0%
!! TGTGTTTAAAATAAAACAGG+TGG - Chr1:62642602-62642621 MsG0180003462.01.T01:intron 25.0%
AAATATCTCGGAGTAATTTG+TGG + Chr1:62642446-62642465 None:intergenic 30.0%
AGAGCTTCAATTAAAAGGTT+CGG - Chr1:62642324-62642343 MsG0180003462.01.T01:intron 30.0%
TTCAATTAAAAGGTTCGGAT+GGG - Chr1:62642329-62642348 MsG0180003462.01.T01:intron 30.0%
! AATATCTCGGAGTAATTTGT+GGG + Chr1:62642445-62642464 None:intergenic 30.0%
! GTATGTTTTCGATTACTACA+TGG - Chr1:62642687-62642706 MsG0180003462.01.T01:CDS 30.0%
! TAATTTTTGTATGCAGCTGT+TGG - Chr1:62641875-62641894 MsG0180003462.01.T01:CDS 30.0%
!! ACATTGGTTCTCAAATATCT+CGG + Chr1:62642458-62642477 None:intergenic 30.0%
AACTTTGGTTGAAATTACCG+TGG - Chr1:62642162-62642181 MsG0180003462.01.T01:CDS 35.0%
ACACATCGACATGTAAAAAG+TGG + Chr1:62641504-62641523 None:intergenic 35.0%
ATAGATGTGGATACACTGTA+TGG - Chr1:62641921-62641940 MsG0180003462.01.T01:CDS 35.0%
CTTCAATTAAAAGGTTCGGA+TGG - Chr1:62642328-62642347 MsG0180003462.01.T01:intron 35.0%
GTAATTTCAACCAAAGTTGC+TGG + Chr1:62642160-62642179 None:intergenic 35.0%
GTGACATTCACAAATAGATG+TGG - Chr1:62641908-62641927 MsG0180003462.01.T01:CDS 35.0%
TCCACGACAACAATATTGAT+CGG + Chr1:62642395-62642414 None:intergenic 35.0%
! CACTTCTCTTCCTTTTCATA+TGG - Chr1:62641536-62641555 MsG0180003462.01.T01:three_prime_UTR 35.0%
!! AAACCTGTTGTTGATAGTTG+AGG + Chr1:62641974-62641993 None:intergenic 35.0%
!! ACCTTTTAATTGAAGCTCTG+CGG + Chr1:62642323-62642342 None:intergenic 35.0%
!!! ATCAACAACAGGTTTTGAGT+TGG - Chr1:62641979-62641998 MsG0180003462.01.T01:CDS 35.0%
!!! TCAACAACAGGTTTTGAGTT+GGG - Chr1:62641980-62641999 MsG0180003462.01.T01:CDS 35.0%
AAGCCTCAACTATCAACAAC+AGG - Chr1:62641968-62641987 MsG0180003462.01.T01:CDS 40.0%
AATGGCCACAATTCATTGGT+GGG + Chr1:62641422-62641441 None:intergenic 40.0%
ACCGATCAATATTGTTGTCG+TGG - Chr1:62642391-62642410 MsG0180003462.01.T01:intron 40.0%
ACCGGATGCTCAAATAACAA+CGG - Chr1:62642064-62642083 MsG0180003462.01.T01:CDS 40.0%
AGCACTTTCACTTGCTCTAA+TGG - Chr1:62642514-62642533 MsG0180003462.01.T01:intron 40.0%
AGCTATAAGCTTCTGGACAT+TGG + Chr1:62642474-62642493 None:intergenic 40.0%
ATCCAATGGCCACAATTCAT+TGG + Chr1:62641426-62641445 None:intergenic 40.0%
ATTTCAACCAAAGTTGCTGG+TGG + Chr1:62642157-62642176 None:intergenic 40.0%
CAATTAGGGACAGAATGTGA+TGG + Chr1:62642551-62642570 None:intergenic 40.0%
GAAATTACCGTGGCATCAAA+TGG - Chr1:62642172-62642191 MsG0180003462.01.T01:CDS 40.0%
GATGCTCAAATAACAACGGA+AGG - Chr1:62642068-62642087 MsG0180003462.01.T01:CDS 40.0%
TAGGCATAGCTATAAGCTTC+TGG + Chr1:62642481-62642500 None:intergenic 40.0%
TCTATTTGTGAATGTCACCG+TGG + Chr1:62641906-62641925 None:intergenic 40.0%
TTTCAACCAAAGTTGCTGGT+GGG + Chr1:62642156-62642175 None:intergenic 40.0%
! AGGCTTTTGATCTCCAGTTA+AGG + Chr1:62641954-62641973 None:intergenic 40.0%
! GATATTTGCTGGACAACTAG+AGG + Chr1:62642287-62642306 None:intergenic 40.0%
! TGTTATTTGAGCATCCGGTT+CGG + Chr1:62642063-62642082 None:intergenic 40.0%
! TTTTTGTATGCAGCTGTTGG+AGG - Chr1:62641878-62641897 MsG0180003462.01.T01:CDS 40.0%
!! CACACAGAGTTGATATTTGC+TGG + Chr1:62642298-62642317 None:intergenic 40.0%
!! CCTTTTAATTGAAGCTCTGC+GGG + Chr1:62642322-62642341 None:intergenic 40.0%
!! TCCGTTGTTATTTGAGCATC+CGG + Chr1:62642068-62642087 None:intergenic 40.0%
!! TGCTTGTAAGAGTGCTTGTT+TGG - Chr1:62642360-62642379 MsG0180003462.01.T01:intron 40.0%
ACAACCCCACCAATGAATTG+TGG - Chr1:62641414-62641433 MsG0180003462.01.T01:three_prime_UTR 45.0%
ATGGCCACAATTCATTGGTG+GGG + Chr1:62641421-62641440 None:intergenic 45.0%
ATTACCGTGGCATCAAATGG+AGG - Chr1:62642175-62642194 MsG0180003462.01.T01:CDS 45.0%
CAATGGCCACAATTCATTGG+TGG + Chr1:62641423-62641442 None:intergenic 45.0%
CACCAATGAATTGTGGCCAT+TGG - Chr1:62641421-62641440 MsG0180003462.01.T01:three_prime_UTR 45.0%
CAGAATGTGATGGCATAGCT+AGG + Chr1:62642541-62642560 None:intergenic 45.0%
CCCGCAGAGCTTCAATTAAA+AGG - Chr1:62642319-62642338 MsG0180003462.01.T01:intron 45.0%
GCTACATATGCTGCATCCAA+TGG + Chr1:62641440-62641459 None:intergenic 45.0%
GTTATTTGAGCATCCGGTTC+GGG + Chr1:62642062-62642081 None:intergenic 45.0%
TGATCTCCAGTTAAGGTTCC+TGG + Chr1:62641947-62641966 None:intergenic 45.0%
TTGTCCTCCATTTGATGCCA+CGG + Chr1:62642182-62642201 None:intergenic 45.0%
TTTCTACGATGTGAGCAACG+TGG - Chr1:62642204-62642223 MsG0180003462.01.T01:CDS 45.0%
! AGTGCTGCTCTTATCATCGT+AGG + Chr1:62642500-62642519 None:intergenic 45.0%
! ATCTCGGAGTAATTTGTGGG+TGG + Chr1:62642442-62642461 None:intergenic 45.0%
!! ACAAGGAGCATCAAGATCAG+TGG - Chr1:62642003-62642022 MsG0180003462.01.T01:CDS 45.0%
!! TCTGGTCAAGTTGACTGCAA+TGG - Chr1:62642118-62642137 MsG0180003462.01.T01:CDS 45.0%
!!! ACAGGTTTTGAGTTGGGACA+AGG - Chr1:62641986-62642005 MsG0180003462.01.T01:CDS 45.0%
!!! TTTTTTTTTTTTTAAAGAAA+GGG + Chr1:62641598-62641617 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTAAAGAA+AGG + Chr1:62641599-62641618 None:intergenic 5.0%
AACTTGACCAGACGCGCAAT+CGG + Chr1:62642110-62642129 None:intergenic 50.0%
GTATGGCCAGGAACCTTAAC+TGG - Chr1:62641938-62641957 MsG0180003462.01.T01:CDS 50.0%
TACGATGTGAGCAACGTGGA+CGG - Chr1:62642208-62642227 MsG0180003462.01.T01:CDS 50.0%
TCGTGGAAGTTACAACACCG+AGG - Chr1:62642408-62642427 MsG0180003462.01.T01:intron 50.0%
TGTGGATACACTGTATGGCC+AGG - Chr1:62641926-62641945 MsG0180003462.01.T01:CDS 50.0%
! CAAGTTGACTGCAATGGTGC+TGG - Chr1:62642124-62642143 MsG0180003462.01.T01:CDS 50.0%
AATCGACCTGACCATGGGGA+AGG + Chr1:62642034-62642053 None:intergenic 55.0%
ATGTCCGTGACTCCACAAGG+CGG - Chr1:62642244-62642263 MsG0180003462.01.T01:CDS 55.0%
CCAATGTCCGTGACTCCACA+AGG - Chr1:62642241-62642260 MsG0180003462.01.T01:CDS 55.0%
CCAGAATCGACCTGACCATG+GGG + Chr1:62642038-62642057 None:intergenic 55.0%
CCCAGAATCGACCTGACCAT+GGG + Chr1:62642039-62642058 None:intergenic 55.0%
CCTTGTGGAGTCACGGACAT+TGG + Chr1:62642244-62642263 None:intergenic 55.0%
CGCAATCCCACCAGCAACTT+TGG - Chr1:62642147-62642166 MsG0180003462.01.T01:CDS 55.0%
! CCCATGGTCAGGTCGATTCT+GGG - Chr1:62642036-62642055 MsG0180003462.01.T01:CDS 55.0%
ACTCCCGCCTTGTGGAGTCA+CGG + Chr1:62642251-62642270 None:intergenic 60.0%
CAGTGGACCTTCCTTCCCCA+TGG - Chr1:62642020-62642039 MsG0180003462.01.T01:CDS 60.0%
GACCTGACCATGGGGAAGGA+AGG + Chr1:62642030-62642049 None:intergenic 60.0%
GACCTTCCTTCCCCATGGTC+AGG - Chr1:62642025-62642044 MsG0180003462.01.T01:CDS 60.0%
GCCCAGAATCGACCTGACCA+TGG + Chr1:62642040-62642059 None:intergenic 60.0%
TGCAGCTGTTGGAGGAGCCA+CGG - Chr1:62641886-62641905 MsG0180003462.01.T01:CDS 60.0%
TGTCCGTGACTCCACAAGGC+GGG - Chr1:62642245-62642264 MsG0180003462.01.T01:CDS 60.0%
TGTGGGTGGACATGTCGCCT+CGG + Chr1:62642428-62642447 None:intergenic 60.0%
! CCCCATGGTCAGGTCGATTC+TGG - Chr1:62642035-62642054 MsG0180003462.01.T01:CDS 60.0%
ACCAGACGCGCAATCGGCTG+TGG + Chr1:62642104-62642123 None:intergenic 65.0%
CAATCGCCACTCCCGCCTTG+TGG + Chr1:62642259-62642278 None:intergenic 65.0%
GTGACTCCACAAGGCGGGAG+TGG - Chr1:62642250-62642269 MsG0180003462.01.T01:CDS 65.0%
! GGTCGATTCTGGGCCCGAAC+CGG - Chr1:62642046-62642065 MsG0180003462.01.T01:CDS 65.0%
GCCACAGCCGATTGCGCGTC+TGG - Chr1:62642100-62642119 MsG0180003462.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 62641407 62642767 62641407 ID=MsG0180003462.01;Name=MsG0180003462.01
Chr1 mRNA 62641407 62642767 62641407 ID=MsG0180003462.01.T01;Parent=MsG0180003462.01;Name=MsG0180003462.01.T01;_AED=0.21;_eAED=0.28;_QI=0|0|0.5|1|1|1|2|200|253
Chr1 exon 62641407 62642283 62641407 ID=MsG0180003462.01.T01:exon:49935;Parent=MsG0180003462.01.T01
Chr1 exon 62642683 62642767 62642683 ID=MsG0180003462.01.T01:exon:49934;Parent=MsG0180003462.01.T01
Chr1 CDS 62642683 62642767 62642683 ID=MsG0180003462.01.T01:cds;Parent=MsG0180003462.01.T01
Chr1 CDS 62641607 62642283 62641607 ID=MsG0180003462.01.T01:cds;Parent=MsG0180003462.01.T01
Chr1 three_prime_UTR 62641407 62641606 62641407 ID=MsG0180003462.01.T01:three_prime_utr;Parent=MsG0180003462.01.T01
Gene Sequence

>MsG0180003462.01.T01

ATGCACGACAACCCCACCAATGAATTGTGGCCATTGGATGCAGCATATGTAGCACAAGATTTATTAGCACTCTCCCATATTTATTCTGTTGGAGGAGCCACGGTGACATTCACAAATAGATGTGGATACACTGTATGGCCAGGAACCTTAACTGGAGATCAAAAGCCTCAACTATCAACAACAGGTTTTGAGTTGGGACAAGGAGCATCAAGATCAGTGGACCTTCCTTCCCCATGGTCAGGTCGATTCTGGGCCCGAACCGGATGCTCAAATAACAACGGAAGGTTTAGTTGTGCCACAGCCGATTGCGCGTCTGGTCAAGTTGACTGCAATGGTGCTGGCGCAATCCCACCAGCAACTTTGGTTGAAATTACCGTGGCATCAAATGGAGGACAAGATTTCTACGATGTGAGCAACGTGGACGGATTCAACGTGCCAATGTCCGTGACTCCACAAGGCGGGAGTGGCGATTGCAAAGCCTCTAGTTGTCCAGCAAATATCAACTCTGTGTGTCCCGCAGAGCTTCAATTAAAAGGTTCGGATGGGAGTGTCGTTGCTTGTAAGAGTGCTTGTTTGGCTTTTAACACCGATCAATATTGTTGTCGTGGAAGTTACAACACCGAGGCGACATGTCCACCCACAAATTACTCCGAGATATTTGAGAACCAATGTCCAGAAGCTTATAGCTATGCCTACGATGATAAGAGCAGCACTTTCACTTGCTCTAATGGACCTAGCTATGCCATCACATTCTGTCCCTAA
Protein sequence

>MsG0180003462.01.T01

MHDNPTNELWPLDAAYVAQDLLALSHIYSVGGATVTFTNRCGYTVWPGTLTGDQKPQLSTTGFELGQGASRSVDLPSPWSGRFWARTGCSNNNGRFSCATADCASGQVDCNGAGAIPPATLVEITVASNGGQDFYDVSNVDGFNVPMSVTPQGGSGDCKASSCPANINSVCPAELQLKGSDGSVVACKSACLAFNTDQYCCRGSYNTEATCPPTNYSEIFENQCPEAYSYAYDDKSSTFTCSNGPSYAITFCP*