AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003559.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003559.01.T01 MTR_1g064430 78.652 356 13 6 1 296 18 370 4.35e-178 509
MsG0180003559.01.T01 MTR_1g064430 78.652 356 13 6 1 296 5 357 6.13e-178 508
MsG0180003559.01.T01 MTR_1g094960 58.160 337 73 10 17 295 13 339 4.31e-117 351
MsG0180003559.01.T01 MTR_4g058930 55.587 349 84 7 20 305 9 349 4.43e-115 347
MsG0180003559.01.T01 MTR_4g058930 55.587 349 84 7 20 305 9 349 6.95e-115 347
MsG0180003559.01.T01 MTR_2g094570 54.755 347 94 7 16 306 4 343 8.27e-115 346
MsG0180003559.01.T01 MTR_2g094570 54.755 347 94 7 16 306 4 343 1.06e-114 347
MsG0180003559.01.T01 MTR_2g094570 53.782 357 102 7 6 306 50 399 1.94e-114 348
MsG0180003559.01.T01 MTR_7g101280 51.714 350 94 8 14 295 22 364 1.00e-104 320
MsG0180003559.01.T01 MTR_0041s0110 51.714 350 94 8 14 295 22 364 1.00e-104 320
MsG0180003559.01.T01 MTR_7g101275 54.266 293 91 7 32 295 1 279 2.61e-95 288
MsG0180003559.01.T01 MTR_0041s0120 50.000 308 93 8 32 295 1 291 1.97e-85 263
MsG0180003559.01.T01 MTR_3g073420 42.773 339 128 8 20 302 16 344 5.89e-68 223
MsG0180003559.01.T01 MTR_5g061220 42.540 315 129 7 18 288 14 320 2.23e-66 217
MsG0180003559.01.T01 MTR_1g058210 37.908 306 143 9 18 292 7 296 4.80e-60 199
MsG0180003559.01.T01 MTR_2g024430 41.667 312 122 12 20 295 17 304 7.78e-60 199
MsG0180003559.01.T01 MTR_5g040880 37.097 310 144 8 17 295 5 294 9.34e-56 189
MsG0180003559.01.T01 MTR_5g040740 39.117 317 136 10 20 301 9 303 2.14e-54 182
MsG0180003559.01.T01 MTR_5g082140 38.585 311 135 10 20 295 9 298 1.54e-51 176
MsG0180003559.01.T01 MTR_4g124900 36.503 326 162 8 11 305 11 322 7.01e-51 181
MsG0180003559.01.T01 MTR_4g124900 36.503 326 162 8 11 305 11 322 7.99e-51 181
MsG0180003559.01.T01 MTR_2g043250 35.276 326 166 7 11 305 11 322 1.52e-49 177
MsG0180003559.01.T01 MTR_2g014770 38.188 309 146 10 19 296 51 345 1.80e-49 174
MsG0180003559.01.T01 MTR_2g014770 38.188 309 146 10 19 296 51 345 5.32e-49 174
MsG0180003559.01.T01 MTR_2g018690 35.890 326 140 9 14 295 14 314 3.14e-48 173
MsG0180003559.01.T01 MTR_5g076270 34.731 334 147 10 17 305 18 325 5.83e-48 172
MsG0180003559.01.T01 MTR_3g064050 34.985 323 141 9 17 295 20 317 9.35e-48 171
MsG0180003559.01.T01 MTR_1g024025 33.433 335 153 8 16 306 36 344 2.73e-47 171
MsG0180003559.01.T01 MTR_8g079492 35.077 325 143 8 14 295 14 313 5.05e-47 169
MsG0180003559.01.T01 MTR_8g079492 35.077 325 143 8 14 295 14 313 5.11e-47 169
MsG0180003559.01.T01 MTR_4g021580 36.364 319 144 8 7 288 3 299 7.01e-47 167
MsG0180003559.01.T01 MTR_4g021580 36.364 319 144 8 7 288 3 299 1.16e-46 167
MsG0180003559.01.T01 MTR_8g100050 35.016 317 162 7 2 287 38 341 1.19e-46 167
MsG0180003559.01.T01 MTR_8g100050 35.016 317 162 7 2 287 38 341 2.20e-46 167
MsG0180003559.01.T01 MTR_2g006270 32.680 306 151 10 22 292 12 297 4.43e-46 162
MsG0180003559.01.T01 MTR_8g101360 35.962 317 156 7 20 303 28 330 5.23e-46 167
MsG0180003559.01.T01 MTR_2g093740 35.826 321 159 10 23 306 56 366 1.16e-45 164
MsG0180003559.01.T01 MTR_2g093740 35.826 321 159 10 23 306 56 366 1.95e-45 164
MsG0180003559.01.T01 MTR_2g093740 35.826 321 159 10 23 306 56 366 2.41e-45 164
MsG0180003559.01.T01 MTR_4g060460 34.906 318 166 7 23 306 54 364 1.29e-44 162
MsG0180003559.01.T01 MTR_4g060460 34.906 318 166 7 23 306 54 364 1.60e-44 162
MsG0180003559.01.T01 MTR_4g088210 34.954 329 152 10 19 305 36 344 5.38e-44 160
MsG0180003559.01.T01 MTR_4g088210 34.954 329 152 10 19 305 36 344 5.91e-44 160
MsG0180003559.01.T01 MTR_2g093740 34.431 334 159 10 23 306 56 379 1.12e-43 159
MsG0180003559.01.T01 MTR_2g093740 34.431 334 159 10 23 306 56 379 1.37e-43 159
MsG0180003559.01.T01 MTR_2g093740 34.431 334 159 10 23 306 56 379 1.55e-43 159
MsG0180003559.01.T01 MTR_8g027440 36.877 301 134 10 23 287 29 309 8.55e-43 156
MsG0180003559.01.T01 MTR_2g005240 35.762 302 135 7 23 286 23 303 1.39e-42 155
MsG0180003559.01.T01 MTR_7g062540 35.647 317 137 12 11 287 4 293 3.92e-42 154
MsG0180003559.01.T01 MTR_5g061890 34.459 296 147 10 20 288 9 284 9.42e-41 147
MsG0180003559.01.T01 MTR_5g060780 34.459 296 147 10 20 288 9 284 2.19e-40 145
MsG0180003559.01.T01 MTR_5g460920 34.459 296 147 10 20 288 9 284 5.15e-40 144
MsG0180003559.01.T01 MTR_2g006380 30.968 310 150 11 20 292 17 299 5.19e-40 145
MsG0180003559.01.T01 MTR_5g062970 38.462 195 86 7 23 189 38 226 1.75e-35 129
MsG0180003559.01.T01 MTR_5g060630 31.562 320 147 11 18 280 7 311 1.66e-31 123
MsG0180003559.01.T01 MTR_5g460740 31.562 320 147 11 18 280 7 311 1.66e-31 123
MsG0180003559.01.T01 MTR_8g079492 34.646 254 103 7 80 295 3 231 1.16e-29 119
MsG0180003559.01.T01 MTR_2g093740 37.981 208 92 5 127 306 38 236 4.05e-28 114
MsG0180003559.01.T01 MTR_2g093740 37.981 208 92 5 127 306 38 236 4.47e-28 114
MsG0180003559.01.T01 MTR_2g093740 35.747 221 92 5 127 306 38 249 2.63e-26 109
MsG0180003559.01.T01 MTR_2g093740 35.747 221 92 5 127 306 38 249 2.87e-26 109
MsG0180003559.01.T01 MTR_8g446900 26.446 242 139 4 69 284 12 240 6.09e-17 80.9
MsG0180003559.01.T01 MTR_5g074840 33.333 144 62 3 127 259 27 147 1.34e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003559.01.T01 AT4G30080 56.548 336 83 10 16 295 12 340 6.85e-112 339
MsG0180003559.01.T01 AT2G28350 53.666 341 94 8 15 295 2 338 5.20e-106 323
MsG0180003559.01.T01 AT2G28350 51.841 353 82 8 15 295 2 338 8.00e-106 324
MsG0180003559.01.T01 AT1G77850 41.590 327 139 7 18 296 14 336 1.18e-72 235
MsG0180003559.01.T01 AT1G77850 41.590 327 139 7 18 296 14 336 1.18e-72 235
MsG0180003559.01.T01 AT5G37020 34.154 325 145 8 15 295 14 313 3.89e-49 175
MsG0180003559.01.T01 AT5G37020 34.154 325 145 8 15 295 14 313 6.21e-49 174
MsG0180003559.01.T01 AT1G19220 36.196 326 163 8 11 305 11 322 8.21e-48 172
MsG0180003559.01.T01 AT5G60450 36.842 304 140 9 23 287 41 331 1.59e-47 170
MsG0180003559.01.T01 AT1G30330 35.671 328 138 10 14 295 14 314 1.79e-47 171
MsG0180003559.01.T01 AT1G30330 35.692 325 136 10 17 295 19 316 1.92e-47 171
MsG0180003559.01.T01 AT1G30330 35.692 325 136 10 17 295 19 316 1.92e-47 171
MsG0180003559.01.T01 AT5G60450 36.842 304 140 9 23 287 65 355 2.20e-47 170
MsG0180003559.01.T01 AT2G33860 36.093 302 147 9 19 288 52 339 1.09e-46 166
MsG0180003559.01.T01 AT5G20730 35.276 326 166 8 11 305 12 323 1.17e-46 169
MsG0180003559.01.T01 AT5G20730 35.168 327 166 9 11 305 12 324 2.61e-45 165
MsG0180003559.01.T01 AT5G20730 35.168 327 166 9 11 305 12 324 2.65e-45 165
MsG0180003559.01.T01 AT1G19850 33.832 334 146 10 20 306 51 356 2.52e-44 162
MsG0180003559.01.T01 AT1G19850 33.832 334 146 10 20 306 40 345 2.64e-44 162
MsG0180003559.01.T01 AT4G23980 34.983 303 138 8 23 287 12 293 5.52e-44 159
MsG0180003559.01.T01 AT4G23980 34.983 303 138 8 23 287 12 293 5.79e-44 159
MsG0180003559.01.T01 AT3G61830 35.127 316 155 11 5 287 7 305 5.88e-44 159
MsG0180003559.01.T01 AT5G62000 35.220 318 154 10 8 287 40 343 6.10e-44 160
MsG0180003559.01.T01 AT5G62000 35.220 318 154 10 8 287 40 343 6.69e-44 160
MsG0180003559.01.T01 AT5G62000 35.220 318 154 10 8 287 40 343 6.69e-44 160
MsG0180003559.01.T01 AT5G62000 35.220 318 154 10 8 287 40 343 6.69e-44 160
MsG0180003559.01.T01 AT5G62000 35.220 318 154 10 8 287 40 343 6.69e-44 160
MsG0180003559.01.T01 AT2G46530 36.093 302 135 10 23 287 21 301 2.63e-42 154
MsG0180003559.01.T01 AT2G46530 35.667 300 139 9 23 287 42 322 3.13e-42 154
MsG0180003559.01.T01 AT1G59750 36.486 296 143 7 23 287 22 303 7.63e-42 154
MsG0180003559.01.T01 AT1G59750 36.486 296 143 7 23 287 22 303 9.20e-42 153
MsG0180003559.01.T01 AT1G59750 36.486 296 143 7 23 287 22 303 9.20e-42 153
MsG0180003559.01.T01 AT1G59750 36.486 296 143 7 23 287 22 303 9.94e-42 153
MsG0180003559.01.T01 AT1G35520 33.115 305 148 8 23 293 27 309 4.23e-37 140
MsG0180003559.01.T01 AT1G34310 33.443 305 147 8 23 293 27 309 6.35e-37 139
MsG0180003559.01.T01 AT1G35540 33.000 300 145 8 23 288 27 304 8.57e-37 139
MsG0180003559.01.T01 AT1G34390 33.443 305 145 10 23 293 27 307 1.79e-35 135
MsG0180003559.01.T01 AT1G35240 32.131 305 151 8 23 293 27 309 2.26e-35 135
MsG0180003559.01.T01 AT1G34410 31.803 305 152 8 23 293 27 309 3.65e-35 134
MsG0180003559.01.T01 AT1G34170 30.392 306 147 10 23 288 25 304 2.01e-31 123
MsG0180003559.01.T01 AT3G61830 36.290 248 114 9 67 287 24 254 6.07e-31 122
MsG0180003559.01.T01 AT1G34170 33.333 225 93 8 23 207 25 232 1.48e-27 112
MsG0180003559.01.T01 AT1G34170 33.333 225 93 8 23 207 25 232 1.57e-27 112
MsG0180003559.01.T01 AT2G33860 36.170 188 87 4 127 288 2 182 5.76e-27 110
MsG0180003559.01.T01 AT5G37020 34.300 207 79 6 127 295 31 218 5.25e-26 108
MsG0180003559.01.T01 AT1G43950 34.450 209 93 7 23 200 27 222 7.53e-25 100
MsG0180003559.01.T01 AT2G46530 37.766 188 82 6 126 287 36 214 1.10e-23 101

Find 83 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCGAATAAGTTGGTTTA+TGG 0.152750 1:+64207766 MsG0180003559.01.T01:CDS
GCATAAACCTCATCGGTTTC+AGG 0.198624 1:-64206973 None:intergenic
TGGGATTCGACGAGCGAAAA+AGG 0.290069 1:+64207396 MsG0180003559.01.T01:CDS
CTTGTTCCTTTGAGTGGAAA+TGG 0.294111 1:+64207006 MsG0180003559.01.T01:CDS
TTGGTTTATGGGATTGTATC+TGG 0.309350 1:+64207778 MsG0180003559.01.T01:CDS
GGACAATTCATGAAGTTAAC+AGG 0.315804 1:-64206895 None:intergenic
TTCACCTTGGAGGCTTCTTC+AGG 0.328238 1:+64207834 MsG0180003559.01.T01:CDS
AGAAGCCTCCAAGGTGAATT+AGG 0.335922 1:-64207829 None:intergenic
ATGCAGATTCGATGGTGTTC+TGG 0.347584 1:+64207707 MsG0180003559.01.T01:CDS
ACCGCTTGAAGGACCGCTTC+TGG 0.350997 1:-64207587 None:intergenic
TCTCGAATAAGTTGGTTTAT+GGG 0.355016 1:+64207767 MsG0180003559.01.T01:CDS
AATGTCTTTGCAAATGAAAC+AGG 0.359372 1:-64207099 None:intergenic
CATGAGGATCCATTACCATT+AGG 0.376847 1:+64207433 MsG0180003559.01.T01:CDS
CCGCTAAAGTTACCGCTTGA+AGG 0.392870 1:-64207598 None:intergenic
TCCAACAGATTCCACCCTAA+TGG 0.396175 1:-64207448 None:intergenic
AGTTTGTTGATCCCCTTCTC+TGG 0.400220 1:+64207806 MsG0180003559.01.T01:CDS
AATCACAAGAAGCTTCTTGC+CGG 0.403468 1:+64207328 MsG0180003559.01.T01:CDS
AATTGTCTCGGCACAATACT+AGG 0.407389 1:-64207162 None:intergenic
AGTGGAAATGGAAATGGAAA+TGG 0.419536 1:+64207018 MsG0180003559.01.T01:CDS
AATGGAAATGGAAATGGAAA+TGG 0.421115 1:+64207024 MsG0180003559.01.T01:CDS
GGCTAATAAGCAACCGTTTG+AGG 0.435944 1:+64207619 MsG0180003559.01.T01:CDS
GGAATCAAAGGTGAAACCTT+TGG 0.447087 1:-64206916 None:intergenic
AACTCAGGCGCGAATATGAC+TGG 0.449187 1:+64207549 MsG0180003559.01.T01:intron
ATGAGGATCCATTACCATTA+GGG 0.450034 1:+64207434 MsG0180003559.01.T01:CDS
CCTTTGAGTGGAAATGGAAA+TGG 0.450640 1:+64207012 MsG0180003559.01.T01:CDS
TCTGGCCTAATTCACCTTGG+AGG 0.451766 1:+64207824 MsG0180003559.01.T01:CDS
TTAAAGCTTGTTCCTTTGAG+TGG 0.471412 1:+64207000 MsG0180003559.01.T01:CDS
TGCATTGCTGCCTCTACTAA+AGG 0.474325 1:-64207689 None:intergenic
CAACTATGGCATGCTTGTGC+AGG 0.483052 1:+64206796 MsG0180003559.01.T01:CDS
AGATTCCACCCTAATGGTAA+TGG 0.485472 1:-64207442 None:intergenic
ATCACAAGAAGCTTCTTGCC+GGG 0.486243 1:+64207329 MsG0180003559.01.T01:CDS
GAAAATGGTGATCTTTGCGT+TGG 0.486357 1:+64207376 MsG0180003559.01.T01:CDS
CCATTTCCATTTCCACTCAA+AGG 0.488607 1:-64207012 None:intergenic
TTAATAGATAAAACTTTGCA+TGG 0.488914 1:-64206937 None:intergenic
AGACGAATCCTCGGTTTCAA+AGG 0.490672 1:-64207747 None:intergenic
TTCTCTGGCCTAATTCACCT+TGG 0.492130 1:+64207821 MsG0180003559.01.T01:CDS
AAAATGGTGATCTTTGCGTT+GGG 0.499819 1:+64207377 MsG0180003559.01.T01:CDS
AAAACTTTGCATGGAATCAA+AGG 0.504526 1:-64206928 None:intergenic
AAATTTAGGCATATTTATAG+AGG 0.504809 1:+64207259 MsG0180003559.01.T01:intron
AGGCGGCATCTGTTGACTAC+TGG 0.519573 1:+64207289 MsG0180003559.01.T01:CDS
TAAACTTTACTGTTCACTGT+TGG 0.522539 1:-64206835 None:intergenic
GGGTAGTAAATAACCTCAAA+CGG 0.524095 1:-64207632 None:intergenic
GGTGTTTAGATCCTCAACTA+TGG 0.524660 1:+64206782 MsG0180003559.01.T01:CDS
AGGATTCGTCTCGAATAAGT+TGG 0.533362 1:+64207759 MsG0180003559.01.T01:CDS
GCATGCTTGTGCAGGTGGAA+TGG 0.542227 1:+64206804 MsG0180003559.01.T01:CDS
CCTTCAAGCGGTAACTTTAG+CGG 0.549169 1:+64207598 MsG0180003559.01.T01:CDS
AAAGTTTATTACTTTCCTCA+AGG 0.553230 1:+64206850 MsG0180003559.01.T01:CDS
CTATGGCATGCTTGTGCAGG+TGG 0.553241 1:+64206799 MsG0180003559.01.T01:CDS
TAACTTAGCATAAACCTCAT+CGG 0.556421 1:-64206980 None:intergenic
AAGGTGAATTAGGCCAGAGA+AGG 0.557096 1:-64207819 None:intergenic
TAACTTCATGAATTGTCCAA+AGG 0.561840 1:+64206900 MsG0180003559.01.T01:CDS
AATTTAGGCATATTTATAGA+GGG 0.569917 1:+64207260 MsG0180003559.01.T01:intron
CAAGATGGCCTTTGAAACCG+AGG 0.577088 1:+64207739 MsG0180003559.01.T01:CDS
GCTGTTGATTATGACACTGT+TGG 0.579285 1:+64207054 MsG0180003559.01.T01:CDS
ATTCATCAGAAGAAGTTGAA+AGG 0.580416 1:+64206761 MsG0180003559.01.T01:CDS
AGGTGAATTAGGCCAGAGAA+GGG 0.583510 1:-64207818 None:intergenic
GGCGCCTTCACACAAAACTC+TGG 0.594083 1:-64207668 None:intergenic
AGGCAGCAATGCAGATTCGA+TGG 0.597819 1:+64207699 MsG0180003559.01.T01:CDS
TGTGAAGGCGCCTTTAGTAG+AGG 0.598970 1:+64207679 MsG0180003559.01.T01:CDS
TCTGATGCTAACAATGGTGG+AGG 0.600675 1:+64207129 MsG0180003559.01.T01:CDS
GTAGTCAACAGATGCCGCCT+CGG 0.601480 1:-64207286 None:intergenic
GTTACCTGAAGAAGCCTCCA+AGG 0.601497 1:-64207838 None:intergenic
TTCTGGAATAAGATTCAAGA+TGG 0.603114 1:+64207724 MsG0180003559.01.T01:CDS
CCAAACGAGGGAAATTGTCT+CGG 0.604007 1:-64207174 None:intergenic
GGGAATCGGTGGTGGACATG+AGG 0.606679 1:+64207417 MsG0180003559.01.T01:CDS
GGCATCTGTTGACTACTGGT+TGG 0.609147 1:+64207293 MsG0180003559.01.T01:CDS
CAATCTGATGCTAACAATGG+TGG 0.611120 1:+64207126 MsG0180003559.01.T01:CDS
AACTCTGGTGTACTTGCACG+AGG 0.611880 1:-64207653 None:intergenic
GCATGTTCAGCATGACCTTG+AGG 0.617891 1:-64206865 None:intergenic
TCACAAGAAGCTTCTTGCCG+GGG 0.620934 1:+64207330 MsG0180003559.01.T01:CDS
GTTGATTATGACACTGTTGG+AGG 0.622867 1:+64207057 MsG0180003559.01.T01:CDS
ACTTATTCGAGACGAATCCT+CGG 0.623357 1:-64207756 None:intergenic
GCACAAGCATGCCATAGTTG+AGG 0.626456 1:-64206793 None:intergenic
GCCAGAAGCGGTCCTTCAAG+CGG 0.635688 1:+64207586 MsG0180003559.01.T01:CDS
CAGGCGCGAATATGACTGGA+GGG 0.648578 1:+64207553 MsG0180003559.01.T01:intron
TCAGGCGCGAATATGACTGG+AGG 0.672052 1:+64207552 MsG0180003559.01.T01:intron
ACTCAATCTGATGCTAACAA+TGG 0.675509 1:+64207123 MsG0180003559.01.T01:CDS
TTTATAGAGGGACACCGAGG+CGG 0.675835 1:+64207272 MsG0180003559.01.T01:CDS
GGCTGATCCTGAAACCGATG+AGG 0.685227 1:+64206966 MsG0180003559.01.T01:CDS
ACTCTGGTGTACTTGCACGA+GGG 0.685503 1:-64207652 None:intergenic
GGTGAATTAGGCCAGAGAAG+GGG 0.693414 1:-64207817 None:intergenic
GGTCATGCTGAACATGCATG+TGG 0.714823 1:+64206871 MsG0180003559.01.T01:CDS
ATATTTATAGAGGGACACCG+AGG 0.780306 1:+64207269 MsG0180003559.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTATCTATTAAGTATA+TGG + Chr1:64206945-64206964 MsG0180003559.01.T01:CDS 15.0%
!! AAATTTAGGCATATTTATAG+AGG + Chr1:64207259-64207278 MsG0180003559.01.T01:intron 20.0%
!! AATTTAGGCATATTTATAGA+GGG + Chr1:64207260-64207279 MsG0180003559.01.T01:intron 20.0%
!! TTAATAGATAAAACTTTGCA+TGG - Chr1:64206940-64206959 None:intergenic 20.0%
! AAAGTTTATTACTTTCCTCA+AGG + Chr1:64206850-64206869 MsG0180003559.01.T01:CDS 25.0%
! ACATTACAACACAATAACAA+TGG + Chr1:64207522-64207541 MsG0180003559.01.T01:intron 25.0%
AAAACTTTGCATGGAATCAA+AGG - Chr1:64206931-64206950 None:intergenic 30.0%
AATAACAATGGTTTGAACTC+AGG + Chr1:64207534-64207553 MsG0180003559.01.T01:intron 30.0%
AATGGAAATGGAAATGGAAA+TGG + Chr1:64207024-64207043 MsG0180003559.01.T01:CDS 30.0%
AATGTCTTTGCAAATGAAAC+AGG - Chr1:64207102-64207121 None:intergenic 30.0%
ACACAGAATGAGAATCAAAT+GGG + Chr1:64207494-64207513 MsG0180003559.01.T01:intron 30.0%
ATTCATCAGAAGAAGTTGAA+AGG + Chr1:64206761-64206780 MsG0180003559.01.T01:CDS 30.0%
ATTCTCATTCTGTGTAAACA+AGG - Chr1:64207489-64207508 None:intergenic 30.0%
TAAACTTTACTGTTCACTGT+TGG - Chr1:64206838-64206857 None:intergenic 30.0%
TAACTTAGCATAAACCTCAT+CGG - Chr1:64206983-64207002 None:intergenic 30.0%
TAACTTCATGAATTGTCCAA+AGG + Chr1:64206900-64206919 MsG0180003559.01.T01:CDS 30.0%
TACACAGAATGAGAATCAAA+TGG + Chr1:64207493-64207512 MsG0180003559.01.T01:intron 30.0%
!! TCTCGAATAAGTTGGTTTAT+GGG + Chr1:64207767-64207786 MsG0180003559.01.T01:CDS 30.0%
!! TTCAATTGTGTTTTTGAGAG+CGG + Chr1:64207354-64207373 MsG0180003559.01.T01:CDS 30.0%
!! TTCTGGAATAAGATTCAAGA+TGG + Chr1:64207724-64207743 MsG0180003559.01.T01:CDS 30.0%
!!! ATTTTGGCTAAAGATGTTCA+CGG + Chr1:64207226-64207245 MsG0180003559.01.T01:intron 30.0%
AAAATGGTGATCTTTGCGTT+GGG + Chr1:64207377-64207396 MsG0180003559.01.T01:CDS 35.0%
ACTCAATCTGATGCTAACAA+TGG + Chr1:64207123-64207142 MsG0180003559.01.T01:CDS 35.0%
AGTGGAAATGGAAATGGAAA+TGG + Chr1:64207018-64207037 MsG0180003559.01.T01:CDS 35.0%
ATGAGGATCCATTACCATTA+GGG + Chr1:64207434-64207453 MsG0180003559.01.T01:CDS 35.0%
ATTCTGTGTAAACAAGGAAG+AGG - Chr1:64207483-64207502 None:intergenic 35.0%
CAAAAACACAATTGAATCCC+CGG - Chr1:64207350-64207369 None:intergenic 35.0%
CACAGAATGAGAATCAAATG+GGG + Chr1:64207495-64207514 MsG0180003559.01.T01:intron 35.0%
GGACAATTCATGAAGTTAAC+AGG - Chr1:64206898-64206917 None:intergenic 35.0%
GGGTAGTAAATAACCTCAAA+CGG - Chr1:64207635-64207654 None:intergenic 35.0%
TTGGTTTATGGGATTGTATC+TGG + Chr1:64207778-64207797 MsG0180003559.01.T01:CDS 35.0%
! TTAAAGCTTGTTCCTTTGAG+TGG + Chr1:64207000-64207019 MsG0180003559.01.T01:CDS 35.0%
!! GTCTCGAATAAGTTGGTTTA+TGG + Chr1:64207766-64207785 MsG0180003559.01.T01:CDS 35.0%
!! TTAGCCAAAATGTTCTGAAG+AGG - Chr1:64207217-64207236 None:intergenic 35.0%
AATCACAAGAAGCTTCTTGC+CGG + Chr1:64207328-64207347 MsG0180003559.01.T01:CDS 40.0%
AATTGTCTCGGCACAATACT+AGG - Chr1:64207165-64207184 None:intergenic 40.0%
ACTTATTCGAGACGAATCCT+CGG - Chr1:64207759-64207778 None:intergenic 40.0%
AGATTCCACCCTAATGGTAA+TGG - Chr1:64207445-64207464 None:intergenic 40.0%
AGGATTCGTCTCGAATAAGT+TGG + Chr1:64207759-64207778 MsG0180003559.01.T01:CDS 40.0%
ATATTTATAGAGGGACACCG+AGG + Chr1:64207269-64207288 MsG0180003559.01.T01:CDS 40.0%
ATGACTGGAGGGAAAAAGTT+AGG + Chr1:64207564-64207583 MsG0180003559.01.T01:CDS 40.0%
CAATCTGATGCTAACAATGG+TGG + Chr1:64207126-64207145 MsG0180003559.01.T01:CDS 40.0%
CATGAGGATCCATTACCATT+AGG + Chr1:64207433-64207452 MsG0180003559.01.T01:CDS 40.0%
CCATTTCCATTTCCACTCAA+AGG - Chr1:64207015-64207034 None:intergenic 40.0%
CCTTTGAGTGGAAATGGAAA+TGG + Chr1:64207012-64207031 MsG0180003559.01.T01:CDS 40.0%
CTTGTTCCTTTGAGTGGAAA+TGG + Chr1:64207006-64207025 MsG0180003559.01.T01:CDS 40.0%
GAAAATGGTGATCTTTGCGT+TGG + Chr1:64207376-64207395 MsG0180003559.01.T01:CDS 40.0%
GGAATCAAAGGTGAAACCTT+TGG - Chr1:64206919-64206938 None:intergenic 40.0%
GGTGTTTAGATCCTCAACTA+TGG + Chr1:64206782-64206801 MsG0180003559.01.T01:CDS 40.0%
GTTGATTATGACACTGTTGG+AGG + Chr1:64207057-64207076 MsG0180003559.01.T01:CDS 40.0%
! GCTGTTGATTATGACACTGT+TGG + Chr1:64207054-64207073 MsG0180003559.01.T01:CDS 40.0%
! TCCTCCTCTTCAGAACATTT+TGG + Chr1:64207210-64207229 MsG0180003559.01.T01:intron 40.0%
!! GTGTTTTTGAGAGCGGAAAA+TGG + Chr1:64207361-64207380 MsG0180003559.01.T01:CDS 40.0%
!! TACACCAGAGTTTTGTGTGA+AGG + Chr1:64207664-64207683 MsG0180003559.01.T01:CDS 40.0%
AAGGTGAATTAGGCCAGAGA+AGG - Chr1:64207822-64207841 None:intergenic 45.0%
ACCATTAGGGTGGAATCTGT+TGG + Chr1:64207447-64207466 MsG0180003559.01.T01:intron 45.0%
AGAAGCCTCCAAGGTGAATT+AGG - Chr1:64207832-64207851 None:intergenic 45.0%
AGACGAATCCTCGGTTTCAA+AGG - Chr1:64207750-64207769 None:intergenic 45.0%
AGGATCCATTACCATTAGGG+TGG + Chr1:64207437-64207456 MsG0180003559.01.T01:intron 45.0%
AGGTGAATTAGGCCAGAGAA+GGG - Chr1:64207821-64207840 None:intergenic 45.0%
ATCACAAGAAGCTTCTTGCC+GGG + Chr1:64207329-64207348 MsG0180003559.01.T01:CDS 45.0%
ATGCAGATTCGATGGTGTTC+TGG + Chr1:64207707-64207726 MsG0180003559.01.T01:CDS 45.0%
CCAAACGAGGGAAATTGTCT+CGG - Chr1:64207177-64207196 None:intergenic 45.0%
CCTTCAAGCGGTAACTTTAG+CGG + Chr1:64207598-64207617 MsG0180003559.01.T01:CDS 45.0%
GGAAAAAGTTAGGCCAGAAG+CGG + Chr1:64207574-64207593 MsG0180003559.01.T01:CDS 45.0%
GGCTAATAAGCAACCGTTTG+AGG + Chr1:64207619-64207638 MsG0180003559.01.T01:CDS 45.0%
TCCAACAGATTCCACCCTAA+TGG - Chr1:64207451-64207470 None:intergenic 45.0%
TCTGATGCTAACAATGGTGG+AGG + Chr1:64207129-64207148 MsG0180003559.01.T01:CDS 45.0%
TGCATTGCTGCCTCTACTAA+AGG - Chr1:64207692-64207711 None:intergenic 45.0%
TTCTCTGGCCTAATTCACCT+TGG + Chr1:64207821-64207840 MsG0180003559.01.T01:CDS 45.0%
TTTCCCTCGTTTGGATTACG+CGG + Chr1:64207183-64207202 MsG0180003559.01.T01:intron 45.0%
!! AGTTTGTTGATCCCCTTCTC+TGG + Chr1:64207806-64207825 MsG0180003559.01.T01:CDS 45.0%
!! GCATAAACCTCATCGGTTTC+AGG - Chr1:64206976-64206995 None:intergenic 45.0%
!! GCCAAAATGTTCTGAAGAGG+AGG - Chr1:64207214-64207233 None:intergenic 45.0%
AACTCAGGCGCGAATATGAC+TGG + Chr1:64207549-64207568 MsG0180003559.01.T01:intron 50.0%
AACTCTGGTGTACTTGCACG+AGG - Chr1:64207656-64207675 None:intergenic 50.0%
ACGGCGAGACGTGGAAATTT+AGG + Chr1:64207245-64207264 MsG0180003559.01.T01:intron 50.0%
ACTCTGGTGTACTTGCACGA+GGG - Chr1:64207655-64207674 None:intergenic 50.0%
AGGCAGCAATGCAGATTCGA+TGG + Chr1:64207699-64207718 MsG0180003559.01.T01:CDS 50.0%
CAACTATGGCATGCTTGTGC+AGG + Chr1:64206796-64206815 MsG0180003559.01.T01:CDS 50.0%
CCGAGACAATTTCCCTCGTT+TGG + Chr1:64207174-64207193 MsG0180003559.01.T01:intron 50.0%
CCGCTAAAGTTACCGCTTGA+AGG - Chr1:64207601-64207620 None:intergenic 50.0%
CGACGAGCGAAAAAGGGAAT+CGG + Chr1:64207403-64207422 MsG0180003559.01.T01:CDS 50.0%
GCACAAGCATGCCATAGTTG+AGG - Chr1:64206796-64206815 None:intergenic 50.0%
GCATGTTCAGCATGACCTTG+AGG - Chr1:64206868-64206887 None:intergenic 50.0%
GGGATTCGACGAGCGAAAAA+GGG + Chr1:64207397-64207416 MsG0180003559.01.T01:CDS 50.0%
GGTCATGCTGAACATGCATG+TGG + Chr1:64206871-64206890 MsG0180003559.01.T01:CDS 50.0%
GGTGAATTAGGCCAGAGAAG+GGG - Chr1:64207820-64207839 None:intergenic 50.0%
TCACAAGAAGCTTCTTGCCG+GGG + Chr1:64207330-64207349 MsG0180003559.01.T01:CDS 50.0%
TCTGGCCTAATTCACCTTGG+AGG + Chr1:64207824-64207843 MsG0180003559.01.T01:CDS 50.0%
TGGGATTCGACGAGCGAAAA+AGG + Chr1:64207396-64207415 MsG0180003559.01.T01:CDS 50.0%
TTCACCTTGGAGGCTTCTTC+AGG + Chr1:64207834-64207853 MsG0180003559.01.T01:CDS 50.0%
TTTATAGAGGGACACCGAGG+CGG + Chr1:64207272-64207291 MsG0180003559.01.T01:CDS 50.0%
! GGCATCTGTTGACTACTGGT+TGG + Chr1:64207293-64207312 MsG0180003559.01.T01:CDS 50.0%
!! CAAGATGGCCTTTGAAACCG+AGG + Chr1:64207739-64207758 MsG0180003559.01.T01:CDS 50.0%
!! TGTGAAGGCGCCTTTAGTAG+AGG + Chr1:64207679-64207698 MsG0180003559.01.T01:CDS 50.0%
AAGATGTTCACGGCGAGACG+TGG + Chr1:64207236-64207255 MsG0180003559.01.T01:intron 55.0%
CAGCCGCGTAATCCAAACGA+GGG - Chr1:64207189-64207208 None:intergenic 55.0%
CAGGCGCGAATATGACTGGA+GGG + Chr1:64207553-64207572 MsG0180003559.01.T01:intron 55.0%
CGAGCGAAAAAGGGAATCGG+TGG + Chr1:64207406-64207425 MsG0180003559.01.T01:CDS 55.0%
CTATGGCATGCTTGTGCAGG+TGG + Chr1:64206799-64206818 MsG0180003559.01.T01:CDS 55.0%
GCATGCTTGTGCAGGTGGAA+TGG + Chr1:64206804-64206823 MsG0180003559.01.T01:CDS 55.0%
GCGAAAAAGGGAATCGGTGG+TGG + Chr1:64207409-64207428 MsG0180003559.01.T01:CDS 55.0%
GGCGCCTTCACACAAAACTC+TGG - Chr1:64207671-64207690 None:intergenic 55.0%
GGCTGATCCTGAAACCGATG+AGG + Chr1:64206966-64206985 MsG0180003559.01.T01:CDS 55.0%
GTAGTCAACAGATGCCGCCT+CGG - Chr1:64207289-64207308 None:intergenic 55.0%
TCAGCCGCGTAATCCAAACG+AGG - Chr1:64207190-64207209 None:intergenic 55.0%
TCAGGCGCGAATATGACTGG+AGG + Chr1:64207552-64207571 MsG0180003559.01.T01:intron 55.0%
! AGGCGGCATCTGTTGACTAC+TGG + Chr1:64207289-64207308 MsG0180003559.01.T01:CDS 55.0%
ACCGCTTGAAGGACCGCTTC+TGG - Chr1:64207590-64207609 None:intergenic 60.0%
GCCAGAAGCGGTCCTTCAAG+CGG + Chr1:64207586-64207605 MsG0180003559.01.T01:CDS 60.0%
!! GGGAATCGGTGGTGGACATG+AGG + Chr1:64207417-64207436 MsG0180003559.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 64206730 64207859 64206730 ID=MsG0180003559.01;Name=MsG0180003559.01
Chr1 mRNA 64206730 64207859 64206730 ID=MsG0180003559.01.T01;Parent=MsG0180003559.01;Name=MsG0180003559.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|317
Chr1 exon 64206730 64207190 64206730 ID=MsG0180003559.01.T01:exon:23743;Parent=MsG0180003559.01.T01
Chr1 exon 64207267 64207455 64207267 ID=MsG0180003559.01.T01:exon:23744;Parent=MsG0180003559.01.T01
Chr1 exon 64207556 64207859 64207556 ID=MsG0180003559.01.T01:exon:23745;Parent=MsG0180003559.01.T01
Chr1 CDS 64206730 64207190 64206730 ID=MsG0180003559.01.T01:cds;Parent=MsG0180003559.01.T01
Chr1 CDS 64207267 64207455 64207267 ID=MsG0180003559.01.T01:cds;Parent=MsG0180003559.01.T01
Chr1 CDS 64207556 64207859 64207556 ID=MsG0180003559.01.T01:cds;Parent=MsG0180003559.01.T01
Gene Sequence

>MsG0180003559.01.T01

ATGGATTCAAGAGATAATAACAATAATAACAATTCATCAGAAGAAGTTGAAAGGTGTTTAGATCCTCAACTATGGCATGCTTGTGCAGGTGGAATGGTTCAGATGCCAACAGTGAACAGTAAAGTTTATTACTTTCCTCAAGGTCATGCTGAACATGCATGTGGACCTGTTAACTTCATGAATTGTCCAAAGGTTTCACCTTTGATTCCATGCAAAGTTTTATCTATTAAGTATATGGCTGATCCTGAAACCGATGAGGTTTATGCTAAGTTAAAGCTTGTTCCTTTGAGTGGAAATGGAAATGGAAATGGAAATGGAAATGATGCTGTTGATTATGACACTGTTGGAGGAAATGAAAATCAAGATAAGCCTGTTTCATTTGCAAAGACATTGACTCAATCTGATGCTAACAATGGTGGAGGTTTTTCTGTTCCTAGTATTGTGCCGAGACAATTTCCCTCGCATATTTATAGAGGGACACCGAGGCGGCATCTGTTGACTACTGGTTGGAGTACTTTTGTTAATCACAAGAAGCTTCTTGCCGGGGATTCAATTGTGTTTTTGAGAGCGGAAAATGGTGATCTTTGCGTTGGGATTCGACGAGCGAAAAAGGGAATCGGTGGTGGACATGAGGATCCATTACCATTAGGGCGCGAATATGACTGGAGGGAAAAAGTTAGGCCAGAAGCGGTCCTTCAAGCGGTAACTTTAGCGGCTAATAAGCAACCGTTTGAGGTTATTTACTACCCTCGTGCAAGTACACCAGAGTTTTGTGTGAAGGCGCCTTTAGTAGAGGCAGCAATGCAGATTCGATGGTGTTCTGGAATAAGATTCAAGATGGCCTTTGAAACCGAGGATTCGTCTCGAATAAGTTGGTTTATGGGATTGTATCTGGAGTTCAGTTTGTTGATCCCCTTCTCTGGCCTAATTCACCTTGGAGGCTTCTTCAGGTAA
Protein sequence

>MsG0180003559.01.T01

MDSRDNNNNNNSSEEVERCLDPQLWHACAGGMVQMPTVNSKVYYFPQGHAEHACGPVNFMNCPKVSPLIPCKVLSIKYMADPETDEVYAKLKLVPLSGNGNGNGNGNDAVDYDTVGGNENQDKPVSFAKTLTQSDANNGGGFSVPSIVPRQFPSHIYRGTPRRHLLTTGWSTFVNHKKLLAGDSIVFLRAENGDLCVGIRRAKKGIGGGHEDPLPLGREYDWREKVRPEAVLQAVTLAANKQPFEVIYYPRASTPEFCVKAPLVEAAMQIRWCSGIRFKMAFETEDSSRISWFMGLYLEFSLLIPFSGLIHLGGFFR*