Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003711.01.T01 | XP_003590511.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 8.93E-90 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003711.01.T01 | P93354 | 80.822 | 146 | 18 | 2 | 1 | 136 | 1 | 146 | 1.16E-76 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003711.01.T01 | G7IBM3 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 4.26e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880042844.01 | MsG0180003711.01 | PPI |
| MsG0480020877.01 | MsG0180003711.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003711.01.T01 | MTR_1g068600 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.08e-93 | 266 |
| MsG0180003711.01.T01 | MTR_4g063240 | 85.034 | 147 | 11 | 2 | 1 | 136 | 1 | 147 | 1.84e-81 | 236 |
| MsG0180003711.01.T01 | MTR_4g063220 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003711.01.T01 | MTR_4g063990 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003711.01.T01 | MTR_4g063200 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003711.01.T01 | MTR_4g064020 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003711.01.T01 | MTR_1g090780 | 91.304 | 138 | 9 | 2 | 1 | 136 | 1 | 137 | 5.32e-78 | 227 |
| MsG0180003711.01.T01 | MTR_1g492910 | 91.304 | 138 | 9 | 2 | 1 | 136 | 1 | 137 | 6.14e-78 | 227 |
| MsG0180003711.01.T01 | MTR_4g071180 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.48e-77 | 225 |
| MsG0180003711.01.T01 | MTR_4g070240 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.48e-77 | 225 |
| MsG0180003711.01.T01 | MTR_4g088025 | 86.713 | 143 | 12 | 2 | 1 | 136 | 1 | 143 | 9.64e-76 | 221 |
| MsG0180003711.01.T01 | MTR_4g070245 | 84.459 | 148 | 11 | 2 | 1 | 136 | 1 | 148 | 1.60e-75 | 221 |
| MsG0180003711.01.T01 | MTR_4g063275 | 83.108 | 148 | 13 | 2 | 1 | 136 | 1 | 148 | 5.06e-75 | 220 |
| MsG0180003711.01.T01 | MTR_4g064983 | 84.722 | 144 | 10 | 2 | 1 | 132 | 1 | 144 | 2.63e-73 | 219 |
| MsG0180003711.01.T01 | MTR_7g099960 | 95.370 | 108 | 4 | 1 | 30 | 136 | 31 | 138 | 3.00e-64 | 192 |
| MsG0180003711.01.T01 | MTR_2g084480 | 62.921 | 89 | 33 | 0 | 47 | 135 | 136 | 224 | 1.02e-37 | 128 |
| MsG0180003711.01.T01 | MTR_8g069990 | 71.591 | 88 | 23 | 2 | 8 | 95 | 3 | 88 | 6.24e-35 | 117 |
| MsG0180003711.01.T01 | MTR_4g063270 | 90.196 | 51 | 5 | 0 | 74 | 124 | 1 | 51 | 5.97e-27 | 95.5 |
| MsG0180003711.01.T01 | MTR_7g114580 | 90.244 | 41 | 4 | 0 | 96 | 136 | 11 | 51 | 7.28e-20 | 77.4 |
| MsG0180003711.01.T01 | MTR_4g032777 | 39.744 | 78 | 47 | 0 | 43 | 120 | 33 | 110 | 1.47e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003711.01.T01 | AT3G45980 | 83.333 | 150 | 11 | 2 | 1 | 136 | 1 | 150 | 6.24e-77 | 225 |
| MsG0180003711.01.T01 | AT2G37470 | 88.406 | 138 | 13 | 2 | 1 | 135 | 1 | 138 | 8.11e-76 | 221 |
| MsG0180003711.01.T01 | AT1G07790 | 83.784 | 148 | 12 | 2 | 1 | 136 | 1 | 148 | 2.43e-75 | 221 |
| MsG0180003711.01.T01 | AT2G28720 | 83.444 | 151 | 10 | 2 | 1 | 136 | 1 | 151 | 2.35e-74 | 218 |
| MsG0180003711.01.T01 | AT3G53650 | 87.681 | 138 | 15 | 2 | 1 | 136 | 1 | 138 | 1.62e-73 | 216 |
| MsG0180003711.01.T01 | AT5G59910 | 87.407 | 135 | 14 | 1 | 5 | 136 | 16 | 150 | 6.45e-73 | 214 |
| MsG0180003711.01.T01 | AT3G46030 | 88.372 | 129 | 12 | 1 | 11 | 136 | 17 | 145 | 1.93e-71 | 211 |
| MsG0180003711.01.T01 | AT5G02570 | 83.571 | 140 | 11 | 2 | 1 | 136 | 1 | 132 | 1.47e-68 | 203 |
| MsG0180003711.01.T01 | AT3G09480 | 80.147 | 136 | 17 | 1 | 1 | 136 | 1 | 126 | 1.28e-65 | 195 |
| MsG0180003711.01.T01 | AT5G22880 | 81.119 | 143 | 17 | 2 | 4 | 136 | 3 | 145 | 2.72e-65 | 195 |
| MsG0180003711.01.T01 | AT1G08170 | 62.353 | 85 | 32 | 0 | 49 | 133 | 151 | 235 | 9.20e-38 | 128 |
Find 35 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAAGATGTAAATCTTAT+AGG | 0.133504 | 1:-67210858 | None:intergenic |
| TAATTAAGAGCTAGTAAATT+TGG | 0.233127 | 1:-67211107 | None:intergenic |
| TTCTTTCGAGATCTTCTTCT+CGG | 0.293193 | 1:-67210792 | None:intergenic |
| AGCGGGTTTCTTCTCTGCCT+TGG | 0.302939 | 1:-67210723 | None:intergenic |
| GCTGTGCGTTTGGTTCTTCC+TGG | 0.313969 | 1:+67211040 | MsG0180003711.01.T01:CDS |
| TTTGAAGAAATACCTATATC+AGG | 0.334896 | 1:-67210899 | None:intergenic |
| GCGGGTTTCTTCTCTGCCTT+GGG | 0.359903 | 1:-67210722 | None:intergenic |
| CATCTTCAAGGTTCTCAAAC+AGG | 0.361477 | 1:+67210871 | MsG0180003711.01.T01:CDS |
| GAGATCTTCTTCTCGGCTCT+TGG | 0.362586 | 1:-67210785 | None:intergenic |
| CTTCTCTGCCTTGGGAGCCA+TGG | 0.363767 | 1:-67210714 | None:intergenic |
| CTATAAGATTTACATCTTCA+AGG | 0.370441 | 1:+67210859 | MsG0180003711.01.T01:CDS |
| GATTCAAACGGCTGTGCGTT+TGG | 0.390239 | 1:+67211030 | MsG0180003711.01.T01:CDS |
| TTTGGTTCTTCCTGGTGAAT+TGG | 0.398260 | 1:+67211048 | MsG0180003711.01.T01:CDS |
| AAACAGGTTCATCCTGATAT+AGG | 0.421612 | 1:+67210887 | MsG0180003711.01.T01:CDS |
| AGGTATTTCTTCAAAAGCTA+TGG | 0.429032 | 1:+67210907 | MsG0180003711.01.T01:CDS |
| AGTAAATTTGGTAACAGCCT+TGG | 0.444512 | 1:-67211095 | None:intergenic |
| CTTGTTGTAACGAGCCAATC+TGG | 0.445538 | 1:-67210984 | None:intergenic |
| TCCGCGGGTTTCTTCTCAGC+GGG | 0.464857 | 1:-67210740 | None:intergenic |
| TCTGCTGGTGATTTCTCCGC+GGG | 0.470002 | 1:-67210755 | None:intergenic |
| CGCTCAGGAAGCTTCCAGAT+TGG | 0.475450 | 1:+67210970 | MsG0180003711.01.T01:CDS |
| GCATGTTTCGCCAATTCACC+AGG | 0.516345 | 1:-67211058 | None:intergenic |
| GGTATTTCTTCAAAAGCTAT+GGG | 0.517184 | 1:+67210908 | MsG0180003711.01.T01:CDS |
| GCGAAACATGCTGTTTCTGA+AGG | 0.530754 | 1:+67211070 | MsG0180003711.01.T01:CDS |
| ATTCAGATCCATGGCTCCCA+AGG | 0.541897 | 1:+67210706 | None:intergenic |
| ATCTCCCTCGACGTAATCGT+CGG | 0.546104 | 1:-67211010 | None:intergenic |
| CTCTGCTGGTGATTTCTCCG+CGG | 0.560516 | 1:-67210756 | None:intergenic |
| GAGAAGAAGATCTCGAAAGA+AGG | 0.564931 | 1:+67210794 | MsG0180003711.01.T01:CDS |
| TATCTTTGAGAAACTCGCTC+AGG | 0.586744 | 1:+67210955 | MsG0180003711.01.T01:CDS |
| TGCTGTTTCTGAAGGAACCA+AGG | 0.603820 | 1:+67211078 | MsG0180003711.01.T01:CDS |
| GAAACCGACGATTACGTCGA+GGG | 0.608632 | 1:+67211006 | MsG0180003711.01.T01:CDS |
| TACGTCGAGGGAGATTCAAA+CGG | 0.609240 | 1:+67211018 | MsG0180003711.01.T01:CDS |
| CTCCGCGGGTTTCTTCTCAG+CGG | 0.628471 | 1:-67210741 | None:intergenic |
| AAGAAGATCTCGAAAGAAGG+AGG | 0.628797 | 1:+67210797 | MsG0180003711.01.T01:CDS |
| ACCCGCTGAGAAGAAACCCG+CGG | 0.680467 | 1:+67210739 | MsG0180003711.01.T01:CDS |
| AGAAACCGACGATTACGTCG+AGG | 0.711553 | 1:+67211005 | MsG0180003711.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTATAAGATTTACATCTTCA+AGG | + | Chr1:67210859-67210878 | MsG0180003711.01.T01:CDS | 25.0% |
| ! | CTTGAAGATGTAAATCTTAT+AGG | - | Chr1:67210861-67210880 | None:intergenic | 25.0% |
| ! | TTTGAAGAAATACCTATATC+AGG | - | Chr1:67210902-67210921 | None:intergenic | 25.0% |
| AGGTATTTCTTCAAAAGCTA+TGG | + | Chr1:67210907-67210926 | MsG0180003711.01.T01:CDS | 30.0% | |
| GGTATTTCTTCAAAAGCTAT+GGG | + | Chr1:67210908-67210927 | MsG0180003711.01.T01:CDS | 30.0% | |
| AAACAGGTTCATCCTGATAT+AGG | + | Chr1:67210887-67210906 | MsG0180003711.01.T01:CDS | 35.0% | |
| TTCTTTCGAGATCTTCTTCT+CGG | - | Chr1:67210795-67210814 | None:intergenic | 35.0% | |
| ! | AGTAAATTTGGTAACAGCCT+TGG | - | Chr1:67211098-67211117 | None:intergenic | 35.0% |
| AAGAAGATCTCGAAAGAAGG+AGG | + | Chr1:67210797-67210816 | MsG0180003711.01.T01:CDS | 40.0% | |
| CATCTTCAAGGTTCTCAAAC+AGG | + | Chr1:67210871-67210890 | MsG0180003711.01.T01:CDS | 40.0% | |
| GAGAAGAAGATCTCGAAAGA+AGG | + | Chr1:67210794-67210813 | MsG0180003711.01.T01:CDS | 40.0% | |
| ! | TATCTTTGAGAAACTCGCTC+AGG | + | Chr1:67210955-67210974 | MsG0180003711.01.T01:CDS | 40.0% |
| !! | TTTGGTTCTTCCTGGTGAAT+TGG | + | Chr1:67211048-67211067 | MsG0180003711.01.T01:CDS | 40.0% |
| TACGTCGAGGGAGATTCAAA+CGG | + | Chr1:67211018-67211037 | MsG0180003711.01.T01:CDS | 45.0% | |
| ! | CTTGTTGTAACGAGCCAATC+TGG | - | Chr1:67210987-67211006 | None:intergenic | 45.0% |
| !! | GCGAAACATGCTGTTTCTGA+AGG | + | Chr1:67211070-67211089 | MsG0180003711.01.T01:CDS | 45.0% |
| !! | TGCTGTTTCTGAAGGAACCA+AGG | + | Chr1:67211078-67211097 | MsG0180003711.01.T01:CDS | 45.0% |
| AGAAACCGACGATTACGTCG+AGG | + | Chr1:67211005-67211024 | MsG0180003711.01.T01:CDS | 50.0% | |
| ATCTCCCTCGACGTAATCGT+CGG | - | Chr1:67211013-67211032 | None:intergenic | 50.0% | |
| GAAACCGACGATTACGTCGA+GGG | + | Chr1:67211006-67211025 | MsG0180003711.01.T01:CDS | 50.0% | |
| GAGATCTTCTTCTCGGCTCT+TGG | - | Chr1:67210788-67210807 | None:intergenic | 50.0% | |
| GATTCAAACGGCTGTGCGTT+TGG | + | Chr1:67211030-67211049 | MsG0180003711.01.T01:CDS | 50.0% | |
| GCATGTTTCGCCAATTCACC+AGG | - | Chr1:67211061-67211080 | None:intergenic | 50.0% | |
| !! | GCTCTTGGCTTTTTCTCTGC+TGG | - | Chr1:67210773-67210792 | None:intergenic | 50.0% |
| CGCTCAGGAAGCTTCCAGAT+TGG | + | Chr1:67210970-67210989 | MsG0180003711.01.T01:CDS | 55.0% | |
| CTCTGCTGGTGATTTCTCCG+CGG | - | Chr1:67210759-67210778 | None:intergenic | 55.0% | |
| GCGGGTTTCTTCTCTGCCTT+GGG | - | Chr1:67210725-67210744 | None:intergenic | 55.0% | |
| TCTGCTGGTGATTTCTCCGC+GGG | - | Chr1:67210758-67210777 | None:intergenic | 55.0% | |
| ! | AGCGGGTTTCTTCTCTGCCT+TGG | - | Chr1:67210726-67210745 | None:intergenic | 55.0% |
| !! | GCTGTGCGTTTGGTTCTTCC+TGG | + | Chr1:67211040-67211059 | MsG0180003711.01.T01:CDS | 55.0% |
| ACCCGCTGAGAAGAAACCCG+CGG | + | Chr1:67210739-67210758 | MsG0180003711.01.T01:CDS | 60.0% | |
| CTCCGCGGGTTTCTTCTCAG+CGG | - | Chr1:67210744-67210763 | None:intergenic | 60.0% | |
| TCCGCGGGTTTCTTCTCAGC+GGG | - | Chr1:67210743-67210762 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 67210716 | 67211126 | 67210716 | ID=MsG0180003711.01;Name=MsG0180003711.01 |
| Chr1 | mRNA | 67210716 | 67211126 | 67210716 | ID=MsG0180003711.01.T01;Parent=MsG0180003711.01;Name=MsG0180003711.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|136 |
| Chr1 | exon | 67210716 | 67211126 | 67210716 | ID=MsG0180003711.01.T01:exon:51549;Parent=MsG0180003711.01.T01 |
| Chr1 | CDS | 67210716 | 67211126 | 67210716 | ID=MsG0180003711.01.T01:cds;Parent=MsG0180003711.01.T01 |
| Gene Sequence |
| Protein sequence |
