AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003737.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003737.01.T01 MTR_1g069055 98.339 301 5 0 1 301 1 301 0.0 619
MsG0180003737.01.T01 MTR_1g069055 85.938 64 8 1 330 392 439 502 2.58e-25 107
MsG0180003737.01.T01 MTR_1g069065 79.932 294 51 4 12 300 1 291 2.27e-167 478
MsG0180003737.01.T01 MTR_1g069065 74.603 63 13 1 330 392 450 509 2.69e-19 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003737.01.T01 AT2G37570 68.876 347 90 8 12 349 1 338 3.11e-156 449
MsG0180003737.01.T01 AT2G37570 79.365 63 10 1 330 392 435 494 1.64e-22 99.4
MsG0180003737.01.T01 AT3G12570 70.164 305 80 8 1 300 1 299 2.47e-142 414
MsG0180003737.01.T01 AT3G12570 62.000 50 19 0 330 379 436 485 3.91e-12 68.2
MsG0180003737.01.T01 AT3G12570 70.164 305 80 8 1 300 1 299 2.47e-142 414
MsG0180003737.01.T01 AT3G12570 62.000 50 19 0 330 379 436 485 3.91e-12 68.2
MsG0180003737.01.T01 AT3G12570 70.164 305 80 8 1 300 1 299 2.47e-142 414
MsG0180003737.01.T01 AT3G12570 62.000 50 19 0 330 379 436 485 3.91e-12 68.2
MsG0180003737.01.T01 AT3G12570 70.164 305 80 8 1 300 1 299 2.47e-142 414
MsG0180003737.01.T01 AT3G12570 62.000 50 19 0 330 379 436 485 3.91e-12 68.2
MsG0180003737.01.T01 AT5G02480 66.770 322 84 10 1 306 1 315 3.59e-139 407
MsG0180003737.01.T01 AT5G02480 74.000 50 12 1 330 378 454 503 3.67e-14 74.3
MsG0180003737.01.T01 AT2G37570 66.995 203 60 2 150 351 1 197 3.22e-83 258
MsG0180003737.01.T01 AT2G37570 79.365 63 10 1 330 392 292 351 6.13e-23 99.4

Find 110 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGACAGCAATGGTGTTTC+GGG 0.210690 1:-67462540 MsG0180003737.01.T01:CDS
TTCTGATCCTTGTGATATTC+TGG 0.218869 1:-67462733 MsG0180003737.01.T01:CDS
GAGCATTTCTTCACCGCTTT+CGG 0.286327 1:+67462657 None:intergenic
AAGAAGCTCCTCAATGAATC+TGG 0.311143 1:-67462066 MsG0180003737.01.T01:CDS
CGAGGCGTGTTGATAGCATT+TGG 0.312491 1:-67462635 MsG0180003737.01.T01:CDS
CGATATCAATTTACCCCTTT+CGG 0.323332 1:-67462772 MsG0180003737.01.T01:CDS
AGCTTCTTTGAGTGAGGAAT+TGG 0.323904 1:+67462081 None:intergenic
GGATGACAGCAATGGTGTTT+CGG 0.325073 1:-67462541 MsG0180003737.01.T01:CDS
GAGGCGTGTTGATAGCATTT+GGG 0.329733 1:-67462634 MsG0180003737.01.T01:CDS
GCTTCAATATTGGCATCTTC+AGG 0.342424 1:+67461578 None:intergenic
TCCTCAGAGCACTGCCCTCC+TGG 0.349557 1:-67461639 MsG0180003737.01.T01:CDS
GGAATTGGAGCACTCTTGAT+TGG 0.366866 1:+67462096 None:intergenic
GAGAGGCCGGAGAATGCTTT+GGG 0.374341 1:-67462438 MsG0180003737.01.T01:CDS
CGAAAGGGGTAAATTGATAT+CGG 0.383055 1:+67462773 None:intergenic
GGAGAGGCCGGAGAATGCTT+TGG 0.404473 1:-67462439 MsG0180003737.01.T01:CDS
CCGAAGTCGCTTGCTTCGGC+CGG 0.412685 1:+67462171 None:intergenic
CTAATCTTTCAAGTCTTGAC+AGG 0.426639 1:-67462248 MsG0180003737.01.T01:CDS
AGTCGAAGGCTAAAGTTACT+AGG 0.430161 1:-67462563 MsG0180003737.01.T01:CDS
TCTCACTTGTTGTCTCTCAA+TGG 0.432740 1:-67462901 MsG0180003737.01.T01:CDS
GATATTCTGGATGTTGGTCT+TGG 0.439937 1:-67462720 MsG0180003737.01.T01:CDS
AGGATCAGTGCTTGAGATCA+TGG 0.440557 1:-67461540 MsG0180003737.01.T01:CDS
ATCATAGTAAGCTTCAATAT+TGG 0.441176 1:+67461568 None:intergenic
CCCTTTCGGTGCCGCAGCCG+TGG 0.445719 1:-67462758 MsG0180003737.01.T01:CDS
TCAGATCCAGGACGAAGTTT+AGG 0.445753 1:+67461515 None:intergenic
GGATGCAAAGGAAGCAGTAC+AGG 0.445854 1:-67462320 MsG0180003737.01.T01:CDS
TTGATAGCATTTGGGGTGCT+TGG 0.452574 1:-67462626 MsG0180003737.01.T01:CDS
TGTTTCGGGGTTTGATAAAA+CGG 0.459919 1:-67462526 MsG0180003737.01.T01:CDS
CCACGGCTGCGGCACCGAAA+GGG 0.460746 1:+67462758 None:intergenic
CTGTCAAGACTTGAAAGATT+AGG 0.468404 1:+67462249 None:intergenic
TGAGCCGAAGTCGCTTGCTT+CGG 0.472282 1:+67462167 None:intergenic
TCAAGCTCAAAATCTGTGTT+CGG 0.478027 1:+67462132 None:intergenic
AATATTGAAGCTTACTATGA+TGG 0.483238 1:-67461566 MsG0180003737.01.T01:CDS
AGATTTGAATTTCATAATCC+CGG 0.485916 1:-67462190 MsG0180003737.01.T01:CDS
CCGGCCGAAGCAAGCGACTT+CGG 0.486024 1:-67462171 MsG0180003737.01.T01:CDS
ATCCAGGTTGCCACAAGATT+AGG 0.488215 1:+67461464 None:intergenic
AAGCAAGCGACTTCGGCTCA+TGG 0.488234 1:-67462164 MsG0180003737.01.T01:CDS
GCACTCTTGATTGGAGGAGG+TGG 0.489975 1:+67462105 None:intergenic
GTCCTAATCTTGTGGCAACC+TGG 0.491519 1:-67461466 MsG0180003737.01.T01:CDS
CGAAGTCGCTTGCTTCGGCC+GGG 0.491525 1:+67462172 None:intergenic
ATGATCTCAAGCACTGATCC+TGG 0.497389 1:+67461542 None:intergenic
CCTTGTGATATTCTGGATGT+TGG 0.504346 1:-67462726 MsG0180003737.01.T01:CDS
GGATTATGAAATTCAAATCT+CGG 0.507468 1:+67462193 None:intergenic
TCCATTGACAAAAGTGTTGA+AGG 0.508193 1:+67462864 None:intergenic
TCCACGGCTGCGGCACCGAA+AGG 0.508880 1:+67462757 None:intergenic
TGATTTAGAGATGAATAGAC+AGG 0.512853 1:-67462814 MsG0180003737.01.T01:CDS
AATTTATCAAGAGAAATGAT+AGG 0.515148 1:-67461683 MsG0180003737.01.T01:CDS
ACTTCATGCTCTTCAGATCC+AGG 0.524848 1:+67461503 None:intergenic
ATGGTGCCTAAACTTCGTCC+TGG 0.529802 1:-67461521 MsG0180003737.01.T01:CDS
GAAAGGGGTAAATTGATATC+GGG 0.534951 1:+67462774 None:intergenic
AGAAGCTCCTCAATGAATCT+GGG 0.536327 1:-67462065 MsG0180003737.01.T01:CDS
GTTTCCTTTCAGTGTTGACA+AGG 0.536419 1:-67462361 MsG0180003737.01.T01:CDS
ATTGGAGCACTCTTGATTGG+AGG 0.540765 1:+67462099 None:intergenic
TTGTCAACACTGAAAGGAAA+CGG 0.540825 1:+67462363 None:intergenic
GATGTTGGTCTTGGTACTCA+AGG 0.541681 1:-67462711 MsG0180003737.01.T01:CDS
ACAGGGATGAGCAGAAGAAG+TGG 0.546941 1:-67462230 MsG0180003737.01.T01:CDS
TAATCTTGTGGCAACCTGGA+TGG 0.548284 1:-67461462 MsG0180003737.01.T01:CDS
GTCGAAGGCTAAAGTTACTA+GGG 0.549025 1:-67462562 MsG0180003737.01.T01:CDS
TTTCGCGAAACAAATTCTCC+AGG 0.552136 1:+67461621 None:intergenic
CACGGCTGCGGCACCGAAAG+GGG 0.553443 1:+67462759 None:intergenic
GGAGCACTCTTGATTGGAGG+AGG 0.554830 1:+67462102 None:intergenic
TTGAGGAGCTTCTTTGAGTG+AGG 0.556306 1:+67462075 None:intergenic
ATCTTCCCCAAAGCATTCTC+CGG 0.557166 1:+67462432 None:intergenic
TCAGGAATACGAGTTAAAAG+AGG 0.557588 1:+67461596 None:intergenic
GGAGGGCAGTGCTCTGAGGA+TGG 0.561491 1:+67461642 None:intergenic
TTCGATCTCATAGGATGCAA+AGG 0.561754 1:-67462332 MsG0180003737.01.T01:CDS
GGATCAGAATTCCACGGCTG+CGG 0.563668 1:+67462747 None:intergenic
AAACCCTTGTCAACACTGAA+AGG 0.565278 1:+67462357 None:intergenic
CTGCCTTCGTCCTAATCTTG+TGG 0.568896 1:-67461474 MsG0180003737.01.T01:CDS
CAGGAATACGAGTTAAAAGA+GGG 0.571303 1:+67461597 None:intergenic
CAACATGAGATCTACCATCC+AGG 0.571701 1:+67461448 None:intergenic
TTTGCTTAACCTTCCGAAAG+CGG 0.572823 1:-67462670 MsG0180003737.01.T01:CDS
ATTTCTTCACCGCTTTCGGA+AGG 0.577148 1:+67462661 None:intergenic
AAGGAAACGGACGTTCCCCT+TGG 0.581022 1:+67462376 None:intergenic
AATTCAGCCCAGATTCATTG+AGG 0.581944 1:+67462058 None:intergenic
AGGCGTGTTGATAGCATTTG+GGG 0.585611 1:-67462633 MsG0180003737.01.T01:CDS
TCAAATCCTCAGTGTCTGCA+TGG 0.586490 1:-67462291 MsG0180003737.01.T01:CDS
TGAACGGACATTCTCGCCAA+GGG 0.598697 1:-67462392 MsG0180003737.01.T01:CDS
GATTAGGACGAAGGCAGACA+CGG 0.610183 1:+67461480 None:intergenic
TTGTCTACTCATATGCCTAA+CGG 0.610882 1:-67462036 MsG0180003737.01.T01:intron
GATGCAAAGGAAGCAGTACA+GGG 0.614610 1:-67462319 MsG0180003737.01.T01:CDS
AAGAAGTGGATGGAACTCAC+TGG 0.615287 1:-67462216 MsG0180003737.01.T01:CDS
CTTTGGGGAAGATGCAGCTG+AGG 0.615425 1:-67462422 MsG0180003737.01.T01:CDS
CCATTGACAAAAGTGTTGAA+GGG 0.616820 1:+67462865 None:intergenic
GGATGAGCAGAAGAAGTGGA+TGG 0.618142 1:-67462226 MsG0180003737.01.T01:CDS
CGCGAAACAAATTCTCCAGG+AGG 0.618513 1:+67461624 None:intergenic
TTTCCTTTCAGTGTTGACAA+GGG 0.621299 1:-67462360 MsG0180003737.01.T01:CDS
TCCAGGAGGGCAGTGCTCTG+AGG 0.623691 1:+67461638 None:intergenic
ATTTGGAAAACATGTACATG+TGG 0.624344 1:-67462470 MsG0180003737.01.T01:CDS
ATGACAGCAATGGTGTTTCG+GGG 0.628591 1:-67462539 MsG0180003737.01.T01:CDS
GAAGCTTACTATGATGGACC+AGG 0.634791 1:-67461560 MsG0180003737.01.T01:CDS
GCGAAACAAATTCTCCAGGA+GGG 0.637929 1:+67461625 None:intergenic
ATGAACGGACATTCTCGCCA+AGG 0.639384 1:-67462393 MsG0180003737.01.T01:CDS
GTTGGTCTTGGTACTCAAGG+TGG 0.640587 1:-67462708 MsG0180003737.01.T01:CDS
TGAAGAGGTAATTCAATGGG+TGG 0.644069 1:-67462930 None:intergenic
TAATCTTTCAAGTCTTGACA+GGG 0.648340 1:-67462247 MsG0180003737.01.T01:CDS
AGTGTTGCTTGAAAAGTCGA+AGG 0.649833 1:-67462577 MsG0180003737.01.T01:CDS
TTGCCACAAGATTAGGACGA+AGG 0.660096 1:+67461471 None:intergenic
GTTACTAGGGATGACAGCAA+TGG 0.662440 1:-67462549 MsG0180003737.01.T01:CDS
TCACAAGGATCAGAATTCCA+CGG 0.663000 1:+67462741 None:intergenic
AGGGGTAAATTGATATCGGG+CGG 0.670799 1:+67462777 None:intergenic
AGAGGCCGGAGAATGCTTTG+GGG 0.673562 1:-67462437 MsG0180003737.01.T01:CDS
CCAACATCCAGAATATCACA+AGG 0.679771 1:+67462726 None:intergenic
GAACGGACATTCTCGCCAAG+GGG 0.681600 1:-67462391 MsG0180003737.01.T01:CDS
CAAGCGACTTCGGCTCATGG+AGG 0.693168 1:-67462161 MsG0180003737.01.T01:CDS
TTTGGAAAACATGTACATGT+GGG 0.696963 1:-67462469 MsG0180003737.01.T01:CDS
AAGCGGTGAAGAAATGCTCG+AGG 0.699893 1:-67462653 MsG0180003737.01.T01:CDS
CAGCTGAGGAGTTACATGAA+CGG 0.706577 1:-67462408 MsG0180003737.01.T01:CDS
ATGCAAAGGAAGCAGTACAG+GGG 0.725658 1:-67462318 MsG0180003737.01.T01:CDS
TCAATACCATGCAGACACTG+AGG 0.729936 1:+67462285 None:intergenic
TTGACAAAAGTGTTGAAGGG+TGG 0.732305 1:+67462868 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATCAAGAGAAATGAT+AGG - Chr1:67462674-67462693 MsG0180003737.01.T01:CDS 20.0%
!!! TCTGAATAATGATGTTTTTA+TGG - Chr1:67461855-67461874 MsG0180003737.01.T01:intron 20.0%
! AATATTGAAGCTTACTATGA+TGG - Chr1:67462791-67462810 MsG0180003737.01.T01:CDS 25.0%
! AATTCTTCATCATTCCATTA+AGG + Chr1:67462396-67462415 None:intergenic 25.0%
! AGATTTGAATTTCATAATCC+CGG - Chr1:67462167-67462186 MsG0180003737.01.T01:CDS 25.0%
! ATCATAGTAAGCTTCAATAT+TGG + Chr1:67462792-67462811 None:intergenic 25.0%
! GGATTATGAAATTCAAATCT+CGG + Chr1:67462167-67462186 None:intergenic 25.0%
ATTTGGAAAACATGTACATG+TGG - Chr1:67461887-67461906 MsG0180003737.01.T01:intron 30.0%
CAGTGCAAAAACAATTTATG+AGG - Chr1:67462526-67462545 MsG0180003737.01.T01:CDS 30.0%
TGATTTAGAGATGAATAGAC+AGG - Chr1:67461543-67461562 MsG0180003737.01.T01:CDS 30.0%
TGGAATGATGAAGAATTTAC+TGG - Chr1:67462399-67462418 MsG0180003737.01.T01:CDS 30.0%
TTTGGAAAACATGTACATGT+GGG - Chr1:67461888-67461907 MsG0180003737.01.T01:intron 30.0%
! TAATCTTTCAAGTCTTGACA+GGG - Chr1:67462110-67462129 MsG0180003737.01.T01:CDS 30.0%
! TTTTATGGTTCAGCATGATT+TGG - Chr1:67461870-67461889 MsG0180003737.01.T01:intron 30.0%
AACAATTTATGAGGAGAGCA+AGG - Chr1:67462535-67462554 MsG0180003737.01.T01:CDS 35.0%
CAGGAATACGAGTTAAAAGA+GGG + Chr1:67462763-67462782 None:intergenic 35.0%
CGAAAGGGGTAAATTGATAT+CGG + Chr1:67461587-67461606 None:intergenic 35.0%
CGATATCAATTTACCCCTTT+CGG - Chr1:67461585-67461604 MsG0180003737.01.T01:CDS 35.0%
CTGTCAAGACTTGAAAGATT+AGG + Chr1:67462111-67462130 None:intergenic 35.0%
GAAAGGGGTAAATTGATATC+GGG + Chr1:67461586-67461605 None:intergenic 35.0%
TCAAGCTCAAAATCTGTGTT+CGG + Chr1:67462228-67462247 None:intergenic 35.0%
TCAGGAATACGAGTTAAAAG+AGG + Chr1:67462764-67462783 None:intergenic 35.0%
TCTCACTCATGGTATCATAA+AGG - Chr1:67462624-67462643 MsG0180003737.01.T01:CDS 35.0%
TGTTTCGGGGTTTGATAAAA+CGG - Chr1:67461831-67461850 MsG0180003737.01.T01:intron 35.0%
TTCCTAGTGTCAAAGTTACT+TGG - Chr1:67462593-67462612 MsG0180003737.01.T01:CDS 35.0%
TTCTGATCCTTGTGATATTC+TGG - Chr1:67461624-67461643 MsG0180003737.01.T01:CDS 35.0%
TTGTCAACACTGAAAGGAAA+CGG + Chr1:67461997-67462016 None:intergenic 35.0%
TTGTCTACTCATATGCCTAA+CGG - Chr1:67462321-67462340 MsG0180003737.01.T01:CDS 35.0%
! CATTATCAGTTTGCCTTTTG+TGG - Chr1:67462567-67462586 MsG0180003737.01.T01:CDS 35.0%
! CTAATCTTTCAAGTCTTGAC+AGG - Chr1:67462109-67462128 MsG0180003737.01.T01:CDS 35.0%
!! AACACTGGTTGAATGCTTTT+AGG - Chr1:67462475-67462494 MsG0180003737.01.T01:CDS 35.0%
!! ACACTGGTTGAATGCTTTTA+GGG - Chr1:67462476-67462495 MsG0180003737.01.T01:CDS 35.0%
!! AGGAAGGACTTTTTCCTTAA+TGG - Chr1:67462379-67462398 MsG0180003737.01.T01:CDS 35.0%
!! CCATTGACAAAAGTGTTGAA+GGG + Chr1:67461495-67461514 None:intergenic 35.0%
!! CTTTTGTCAATGGATTCAAG+TGG - Chr1:67461502-67461521 MsG0180003737.01.T01:CDS 35.0%
!! TCCATTGACAAAAGTGTTGA+AGG + Chr1:67461496-67461515 None:intergenic 35.0%
!! TTTCCTTTCAGTGTTGACAA+GGG - Chr1:67461997-67462016 MsG0180003737.01.T01:intron 35.0%
!!! AATTGTTTTTGCACTGTAGC+CGG + Chr1:67462522-67462541 None:intergenic 35.0%
AAACCCTTGTCAACACTGAA+AGG + Chr1:67462003-67462022 None:intergenic 40.0%
AAGAAGCTCCTCAATGAATC+TGG - Chr1:67462291-67462310 MsG0180003737.01.T01:CDS 40.0%
AATTCAGCCCAGATTCATTG+AGG + Chr1:67462302-67462321 None:intergenic 40.0%
ACACTAGGAAGATCCACAAA+AGG + Chr1:67462583-67462602 None:intergenic 40.0%
ACTAGGGTGCATTTCAAATC+CGG + Chr1:67462451-67462470 None:intergenic 40.0%
AGAAGCTCCTCAATGAATCT+GGG - Chr1:67462292-67462311 MsG0180003737.01.T01:CDS 40.0%
AGCTTCTTTGAGTGAGGAAT+TGG + Chr1:67462279-67462298 None:intergenic 40.0%
AGTCGAAGGCTAAAGTTACT+AGG - Chr1:67461794-67461813 MsG0180003737.01.T01:intron 40.0%
CCAACATCCAGAATATCACA+AGG + Chr1:67461634-67461653 None:intergenic 40.0%
CCTTGTGATATTCTGGATGT+TGG - Chr1:67461631-67461650 MsG0180003737.01.T01:CDS 40.0%
CTAGTGTCAAAGTTACTTGG+AGG - Chr1:67462596-67462615 MsG0180003737.01.T01:CDS 40.0%
CTCCAAGTAACTTTGACACT+AGG + Chr1:67462598-67462617 None:intergenic 40.0%
CTGTTAATCCTCCATCTGAT+CGG - Chr1:67462422-67462441 MsG0180003737.01.T01:CDS 40.0%
CTTCCTTCTTGCTACTGATA+GGG + Chr1:67462365-67462384 None:intergenic 40.0%
GCTTCAATATTGGCATCTTC+AGG + Chr1:67462782-67462801 None:intergenic 40.0%
GTCGAAGGCTAAAGTTACTA+GGG - Chr1:67461795-67461814 MsG0180003737.01.T01:intron 40.0%
TCACAAGGATCAGAATTCCA+CGG + Chr1:67461619-67461638 None:intergenic 40.0%
TGATGAAGAATGTCTTGGAC+CGG - Chr1:67462500-67462519 MsG0180003737.01.T01:CDS 40.0%
TTCCTTCTTGCTACTGATAG+GGG + Chr1:67462364-67462383 None:intergenic 40.0%
TTCGATCTCATAGGATGCAA+AGG - Chr1:67462025-67462044 MsG0180003737.01.T01:intron 40.0%
TTTCGCGAAACAAATTCTCC+AGG + Chr1:67462739-67462758 None:intergenic 40.0%
TTTGCTTAACCTTCCGAAAG+CGG - Chr1:67461687-67461706 MsG0180003737.01.T01:CDS 40.0%
! CATGTACATGTGGGTTTTCA+AGG - Chr1:67461897-67461916 MsG0180003737.01.T01:intron 40.0%
! CCCTTCAACACTTTTGTCAA+TGG - Chr1:67461492-67461511 MsG0180003737.01.T01:CDS 40.0%
! GATATTCTGGATGTTGGTCT+TGG - Chr1:67461637-67461656 MsG0180003737.01.T01:CDS 40.0%
! TCAACCAGTGTTGTTCACTA+GGG + Chr1:67462467-67462486 None:intergenic 40.0%
! TTCAACCAGTGTTGTTCACT+AGG + Chr1:67462468-67462487 None:intergenic 40.0%
!! AGTGTTGCTTGAAAAGTCGA+AGG - Chr1:67461780-67461799 MsG0180003737.01.T01:intron 40.0%
!! CACTGGTTGAATGCTTTTAG+GGG - Chr1:67462477-67462496 MsG0180003737.01.T01:CDS 40.0%
!! GTTTCCTTTCAGTGTTGACA+AGG - Chr1:67461996-67462015 MsG0180003737.01.T01:intron 40.0%
!! TCTCACTTGTTGTCTCTCAA+TGG - Chr1:67461456-67461475 MsG0180003737.01.T01:CDS 40.0%
!! TTCGACTTTTCAAGCAACAC+TGG + Chr1:67461781-67461800 None:intergenic 40.0%
!! TTGACAAAAGTGTTGAAGGG+TGG + Chr1:67461492-67461511 None:intergenic 40.0%
!!! AGGGTTTTGTTCGATCTCAT+AGG - Chr1:67462016-67462035 MsG0180003737.01.T01:intron 40.0%
AAATCCGGAATCCGATCAGA+TGG + Chr1:67462436-67462455 None:intergenic 45.0%
AAGAAGTGGATGGAACTCAC+TGG - Chr1:67462141-67462160 MsG0180003737.01.T01:CDS 45.0%
ACTTCATGCTCTTCAGATCC+AGG + Chr1:67462857-67462876 None:intergenic 45.0%
AGGATCAGTGCTTGAGATCA+TGG - Chr1:67462817-67462836 MsG0180003737.01.T01:CDS 45.0%
AGGGGTAAATTGATATCGGG+CGG + Chr1:67461583-67461602 None:intergenic 45.0%
AGGGGTGATGAAGAATGTCT+TGG - Chr1:67462495-67462514 MsG0180003737.01.T01:CDS 45.0%
ATCCAGGTTGCCACAAGATT+AGG + Chr1:67462896-67462915 None:intergenic 45.0%
ATGACAGCAATGGTGTTTCG+GGG - Chr1:67461818-67461837 MsG0180003737.01.T01:intron 45.0%
ATGCAAAGGAAGCAGTACAG+GGG - Chr1:67462039-67462058 MsG0180003737.01.T01:CDS 45.0%
ATTTCTTCACCGCTTTCGGA+AGG + Chr1:67461699-67461718 None:intergenic 45.0%
CAACATGAGATCTACCATCC+AGG + Chr1:67462912-67462931 None:intergenic 45.0%
CAGCTGAGGAGTTACATGAA+CGG - Chr1:67461949-67461968 MsG0180003737.01.T01:intron 45.0%
CCTATCAGTAGCAAGAAGGA+AGG - Chr1:67462363-67462382 MsG0180003737.01.T01:CDS 45.0%
CCTTCCTTCTTGCTACTGAT+AGG + Chr1:67462366-67462385 None:intergenic 45.0%
GAGCATTTCTTCACCGCTTT+CGG + Chr1:67461703-67461722 None:intergenic 45.0%
GATGACAGCAATGGTGTTTC+GGG - Chr1:67461817-67461836 MsG0180003737.01.T01:intron 45.0%
GATGCAAAGGAAGCAGTACA+GGG - Chr1:67462038-67462057 MsG0180003737.01.T01:CDS 45.0%
GCGAAACAAATTCTCCAGGA+GGG + Chr1:67462735-67462754 None:intergenic 45.0%
GGATGACAGCAATGGTGTTT+CGG - Chr1:67461816-67461835 MsG0180003737.01.T01:intron 45.0%
GTTACTAGGGATGACAGCAA+TGG - Chr1:67461808-67461827 MsG0180003737.01.T01:intron 45.0%
TAATCTTGTGGCAACCTGGA+TGG - Chr1:67462895-67462914 MsG0180003737.01.T01:CDS 45.0%
TACTCATATGCCTAACGGTG+AGG - Chr1:67462326-67462345 MsG0180003737.01.T01:CDS 45.0%
TCAAATCCTCAGTGTCTGCA+TGG - Chr1:67462066-67462085 MsG0180003737.01.T01:CDS 45.0%
TCAATACCATGCAGACACTG+AGG + Chr1:67462075-67462094 None:intergenic 45.0%
TCAGATCCAGGACGAAGTTT+AGG + Chr1:67462845-67462864 None:intergenic 45.0%
TGGAGGAATACTCTCACTCA+TGG - Chr1:67462613-67462632 MsG0180003737.01.T01:CDS 45.0%
TTGAGGAGCTTCTTTGAGTG+AGG + Chr1:67462285-67462304 None:intergenic 45.0%
TTGCCACAAGATTAGGACGA+AGG + Chr1:67462889-67462908 None:intergenic 45.0%
! ATCTTCCCCAAAGCATTCTC+CGG + Chr1:67461928-67461947 None:intergenic 45.0%
! ATTGGAGCACTCTTGATTGG+AGG + Chr1:67462261-67462280 None:intergenic 45.0%
! GGAATTGGAGCACTCTTGAT+TGG + Chr1:67462264-67462283 None:intergenic 45.0%
! TTGATAGCATTTGGGGTGCT+TGG - Chr1:67461731-67461750 MsG0180003737.01.T01:intron 45.0%
!! ACATGTGGGTTTTCAAGGAG+AGG - Chr1:67461902-67461921 MsG0180003737.01.T01:intron 45.0%
!! AGGCGTGTTGATAGCATTTG+GGG - Chr1:67461724-67461743 MsG0180003737.01.T01:intron 45.0%
!! ATGATCTCAAGCACTGATCC+TGG + Chr1:67462818-67462837 None:intergenic 45.0%
!! GAAGCTTACTATGATGGACC+AGG - Chr1:67462797-67462816 MsG0180003737.01.T01:CDS 45.0%
!! GAGGCGTGTTGATAGCATTT+GGG - Chr1:67461723-67461742 MsG0180003737.01.T01:intron 45.0%
!! GATGTTGGTCTTGGTACTCA+AGG - Chr1:67461646-67461665 MsG0180003737.01.T01:CDS 45.0%
!! GGGGTGCTTGGTTTTTCTTT+AGG - Chr1:67461743-67461762 MsG0180003737.01.T01:intron 45.0%
AAGCGGTGAAGAAATGCTCG+AGG - Chr1:67461704-67461723 MsG0180003737.01.T01:intron 50.0%
ACAGGGATGAGCAGAAGAAG+TGG - Chr1:67462127-67462146 MsG0180003737.01.T01:CDS 50.0%
ATGAACGGACATTCTCGCCA+AGG - Chr1:67461964-67461983 MsG0180003737.01.T01:intron 50.0%
ATGGTGCCTAAACTTCGTCC+TGG - Chr1:67462836-67462855 MsG0180003737.01.T01:CDS 50.0%
CGCGAAACAAATTCTCCAGG+AGG + Chr1:67462736-67462755 None:intergenic 50.0%
CTGCCTTCGTCCTAATCTTG+TGG - Chr1:67462883-67462902 MsG0180003737.01.T01:CDS 50.0%
GATTAGGACGAAGGCAGACA+CGG + Chr1:67462880-67462899 None:intergenic 50.0%
GGATGAGCAGAAGAAGTGGA+TGG - Chr1:67462131-67462150 MsG0180003737.01.T01:CDS 50.0%
GGATGCAAAGGAAGCAGTAC+AGG - Chr1:67462037-67462056 MsG0180003737.01.T01:CDS 50.0%
GTCCCCTATCAGTAGCAAGA+AGG - Chr1:67462359-67462378 MsG0180003737.01.T01:CDS 50.0%
GTCCTAATCTTGTGGCAACC+TGG - Chr1:67462891-67462910 MsG0180003737.01.T01:CDS 50.0%
TCATATGCCTAACGGTGAGG+CGG - Chr1:67462329-67462348 MsG0180003737.01.T01:CDS 50.0%
TCCTCCATCTGATCGGATTC+CGG - Chr1:67462429-67462448 MsG0180003737.01.T01:CDS 50.0%
TGAACGGACATTCTCGCCAA+GGG - Chr1:67461965-67461984 MsG0180003737.01.T01:intron 50.0%
TGCACCCTAGTGAACAACAC+TGG - Chr1:67462460-67462479 MsG0180003737.01.T01:CDS 50.0%
!! CGAGGCGTGTTGATAGCATT+TGG - Chr1:67461722-67461741 MsG0180003737.01.T01:intron 50.0%
!! GTTGGTCTTGGTACTCAAGG+TGG - Chr1:67461649-67461668 MsG0180003737.01.T01:CDS 50.0%
AAGCAAGCGACTTCGGCTCA+TGG - Chr1:67462193-67462212 MsG0180003737.01.T01:CDS 55.0%
AAGGAAACGGACGTTCCCCT+TGG + Chr1:67461984-67462003 None:intergenic 55.0%
AGAGGCCGGAGAATGCTTTG+GGG - Chr1:67461920-67461939 MsG0180003737.01.T01:intron 55.0%
GAACGGACATTCTCGCCAAG+GGG - Chr1:67461966-67461985 MsG0180003737.01.T01:intron 55.0%
GAGAGGCCGGAGAATGCTTT+GGG - Chr1:67461919-67461938 MsG0180003737.01.T01:intron 55.0%
GCACTCTTGATTGGAGGAGG+TGG + Chr1:67462255-67462274 None:intergenic 55.0%
GGATCAGAATTCCACGGCTG+CGG + Chr1:67461613-67461632 None:intergenic 55.0%
TCCGGAATCCGATCAGATGG+AGG + Chr1:67462433-67462452 None:intergenic 55.0%
TGAGCCGAAGTCGCTTGCTT+CGG + Chr1:67462193-67462212 None:intergenic 55.0%
! CTTTGGGGAAGATGCAGCTG+AGG - Chr1:67461935-67461954 MsG0180003737.01.T01:intron 55.0%
! GGAGCACTCTTGATTGGAGG+AGG + Chr1:67462258-67462277 None:intergenic 55.0%
!! GTGGGTTTTCAAGGAGAGGC+CGG - Chr1:67461906-67461925 MsG0180003737.01.T01:intron 55.0%
CAAGCGACTTCGGCTCATGG+AGG - Chr1:67462196-67462215 MsG0180003737.01.T01:CDS 60.0%
GGAGAGGCCGGAGAATGCTT+TGG - Chr1:67461918-67461937 MsG0180003737.01.T01:intron 60.0%
ACGTCAGCCGCCTCACCGTT+AGG + Chr1:67462339-67462358 None:intergenic 65.0%
CCGAAGTCGCTTGCTTCGGC+CGG + Chr1:67462189-67462208 None:intergenic 65.0%
CCGGCCGAAGCAAGCGACTT+CGG - Chr1:67462186-67462205 MsG0180003737.01.T01:CDS 65.0%
CGAAGTCGCTTGCTTCGGCC+GGG + Chr1:67462188-67462207 None:intergenic 65.0%
TCCAGGAGGGCAGTGCTCTG+AGG + Chr1:67462722-67462741 None:intergenic 65.0%
!! GGAGGGCAGTGCTCTGAGGA+TGG + Chr1:67462718-67462737 None:intergenic 65.0%
!! TCCTCAGAGCACTGCCCTCC+TGG - Chr1:67462718-67462737 MsG0180003737.01.T01:CDS 65.0%
! CACGGCTGCGGCACCGAAAG+GGG + Chr1:67461601-67461620 None:intergenic 70.0%
! CCACGGCTGCGGCACCGAAA+GGG + Chr1:67461602-67461621 None:intergenic 70.0%
! TCCACGGCTGCGGCACCGAA+AGG + Chr1:67461603-67461622 None:intergenic 70.0%
!! CCCTTTCGGTGCCGCAGCCG+TGG - Chr1:67461599-67461618 MsG0180003737.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 67461442 67462937 67461442 ID=MsG0180003737.01;Name=MsG0180003737.01
Chr1 mRNA 67461442 67462937 67461442 ID=MsG0180003737.01.T01;Parent=MsG0180003737.01;Name=MsG0180003737.01.T01;_AED=0.49;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|392
Chr1 exon 67462037 67462937 67462037 ID=MsG0180003737.01.T01:exon:5725;Parent=MsG0180003737.01.T01
Chr1 exon 67461442 67461719 67461442 ID=MsG0180003737.01.T01:exon:5724;Parent=MsG0180003737.01.T01
Chr1 CDS 67462037 67462937 67462037 ID=MsG0180003737.01.T01:cds;Parent=MsG0180003737.01.T01
Chr1 CDS 67461442 67461719 67461442 ID=MsG0180003737.01.T01:cds;Parent=MsG0180003737.01.T01
Gene Sequence

>MsG0180003737.01.T01

ATGGGTGGTTCTCTTCTCACTTGTTGTCTCTCAATGGAGAATCATCACCACCCTTCAACACTTTTGTCAATGGATTCAAGTGGATGTTCTCATGATGAGCTTGATTTAGAGATGAATAGACAGGTTATACTTTCGCGTCCGCCCGATATCAATTTACCCCTTTCGGTGCCGCAGCCGTGGAATTCTGATCCTTGTGATATTCTGGATGTTGGTCTTGGTACTCAAGGTGGTTATGAGACTGAGAGTTTGCTTAACCTTCCGAAAGCGGTGAAGAAATGCTCGAGGCGTGTTGATAGCATTTGGGGTGCTTGGTTTTTCTTTAGGTTTTATTTTAAGCCAGTGTTGCTTGAAAAGTCGAAGGCTAAAGTTACTAGGGATGACAGCAATGGTGTTTCGGGGTTTGATAAAACGGATCTGAATAATGATGTTTTTATGGTTCAGCATGATTTGGAAAACATGTACATGTGGGTTTTCAAGGAGAGGCCGGAGAATGCTTTGGGGAAGATGCAGCTGAGGAGTTACATGAACGGACATTCTCGCCAAGGGGAACGTCCGTTTCCTTTCAGTGTTGACAAGGGTTTTGTTCGATCTCATAGGATGCAAAGGAAGCAGTACAGGGGACTTTCAAATCCTCAGTGTCTGCATGGTATTGAACTTGTTTCGTCGCCTAATCTTTCAAGTCTTGACAGGGATGAGCAGAAGAAGTGGATGGAACTCACTGGCCGAGATTTGAATTTCATAATCCCGGCCGAAGCAAGCGACTTCGGCTCATGGAGGAATCTTCCGAACACAGATTTTGAGCTTGAGAGACCACCTCCTCCAATCAAGAGTGCTCCAATTCCTCACTCAAAGAAGCTCCTCAATGAATCTGGGCTGAATTTGTCTACTCATATGCCTAACGCACATCACGAAAGCAATTTATCAAGAGAAATGATAGGACATTCAAGCTCACAGATCCATCCTCAGAGCACTGCCCTCCTGGAGAATTTGTTTCGCGAAATCCCTCTTTTAACTCGTATTCCTGAAGATGCCAATATTGAAGCTTACTATGATGGACCAGGATCAGTGCTTGAGATCATGGTGCCTAAACTTCGTCCTGGATCTGAAGAGCATGAAGTCCGTGTCTGCCTTCGTCCTAATCTTGTGGCAACCTGGATGGTAGATCTCATGTTGACTTGA
Protein sequence

>MsG0180003737.01.T01

MGGSLLTCCLSMENHHHPSTLLSMDSSGCSHDELDLEMNRQVILSRPPDINLPLSVPQPWNSDPCDILDVGLGTQGGYETESLLNLPKAVKKCSRRVDSIWGAWFFFRFYFKPVLLEKSKAKVTRDDSNGVSGFDKTDLNNDVFMVQHDLENMYMWVFKERPENALGKMQLRSYMNGHSRQGERPFPFSVDKGFVRSHRMQRKQYRGLSNPQCLHGIELVSSPNLSSLDRDEQKKWMELTGRDLNFIIPAEASDFGSWRNLPNTDFELERPPPPIKSAPIPHSKKLLNESGLNLSTHMPNAHHESNLSREMIGHSSSQIHPQSTALLENLFREIPLLTRIPEDANIEAYYDGPGSVLEIMVPKLRPGSEEHEVRVCLRPNLVATWMVDLMLT*