AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003811.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003811.01.T01 MTR_1g069765 99.283 279 2 0 1 279 1 279 0.0 567
MsG0180003811.01.T01 MTR_2g091185 43.017 179 92 3 5 180 18 189 2.31e-41 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003811.01.T01 AT1G03900 70.545 275 62 6 10 279 12 272 1.01e-118 341
MsG0180003811.01.T01 AT3G58600 43.575 179 85 4 5 177 27 195 7.67e-40 140

Find 120 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTCTCTGATTTATTT+TGG 0.077754 1:-68569969 MsG0180003811.01.T01:three_prime_UTR
CAAGGGAAGCATGCGTTTAT+TGG 0.243287 1:-68571587 MsG0180003811.01.T01:CDS
GCTGGCATGCTTTCAGCTGC+TGG 0.245848 1:-68570769 MsG0180003811.01.T01:CDS
ATCGGATCTTTCTCAATTAC+AGG 0.250114 1:-68570554 MsG0180003811.01.T01:intron
GAATTTGAGAAACGCGTTAT+TGG 0.257049 1:+68571974 None:intergenic
GGTCCTGTATCTAGTGTTAA+AGG 0.284346 1:-68570613 MsG0180003811.01.T01:CDS
GACAAATACATTTGGGTTGA+TGG 0.307575 1:+68569682 None:intergenic
GGAGCAAGGTTCAATACTTT+TGG 0.310945 1:+68570715 None:intergenic
TTCTTCTAACGACATCGTTT+TGG 0.328092 1:+68571904 None:intergenic
TTGGGAGAGATCAAGGGATT+GGG 0.335083 1:+68569772 None:intergenic
AAGCATGCGTTTATTGGATT+GGG 0.336786 1:-68571581 MsG0180003811.01.T01:CDS
CACAAGGCGGCTGGTGGAGC+TGG 0.355323 1:-68570787 MsG0180003811.01.T01:CDS
GCCGTGACCGGTGCGAGATC+CGG 0.369388 1:-68571733 MsG0180003811.01.T01:CDS
GCGAAGAGATCACCGGAGTT+AGG 0.375730 1:+68571701 None:intergenic
ACAGTTGAGACAAATACATT+TGG 0.380622 1:+68569674 None:intergenic
GCTTTCAGCTGCTGGCCTAT+CGG 0.386640 1:-68570761 MsG0180003811.01.T01:CDS
CTTTCAGCTGCTGGCCTATC+GGG 0.396419 1:-68570760 MsG0180003811.01.T01:CDS
TCCCGGTCAGCGTGAGGGTT+CGG 0.398042 1:-68571663 MsG0180003811.01.T01:CDS
CGAGCAGCAACCGGATCATT+TGG 0.402087 1:+68570649 None:intergenic
TTCATCCTGCTGTTAATCAT+AGG 0.414816 1:-68571433 MsG0180003811.01.T01:CDS
TGCTGTTAATCATAGGCTTA+AGG 0.419472 1:-68571426 MsG0180003811.01.T01:intron
CAGTTGAGACAAATACATTT+GGG 0.421674 1:+68569675 None:intergenic
AACCGAACCCTCACGCTGAC+CGG 0.428541 1:+68571661 None:intergenic
CTTCCCTCATCAGCCACTCC+TGG 0.429001 1:-68570115 MsG0180003811.01.T01:CDS
GTCCCTTTAACACTAGATAC+AGG 0.431990 1:+68570610 None:intergenic
GAAGCATGCGTTTATTGGAT+TGG 0.436801 1:-68571582 MsG0180003811.01.T01:CDS
GCGTGAGGGTTCGGTTGAAC+CGG 0.442496 1:-68571654 MsG0180003811.01.T01:CDS
TGTGAGAAGAGAACATGATA+AGG 0.442504 1:-68571498 MsG0180003811.01.T01:CDS
TTTGAGAAAAGAAATATTGA+AGG 0.443231 1:-68569935 MsG0180003811.01.T01:three_prime_UTR
GGCTCCAATCAGACAAGATC+TGG 0.444379 1:-68571781 MsG0180003811.01.T01:CDS
CTTGGATCGACAGCTGCGTC+AGG 0.445581 1:-68570041 MsG0180003811.01.T01:three_prime_UTR
ATAAAGATTGGGAGAGATCA+AGG 0.446692 1:+68569765 None:intergenic
ATCCCGCCGCGAACAACCTC+CGG 0.448428 1:-68571824 MsG0180003811.01.T01:CDS
TTTGTTCTGAAGATTGAAGA+TGG 0.449673 1:-68571611 MsG0180003811.01.T01:CDS
ACGCCACAGTCAATAAAGAT+TGG 0.451335 1:+68569753 None:intergenic
CCTGGATCAACACCTGCATC+AGG 0.454323 1:-68570097 MsG0180003811.01.T01:CDS
TGGGAGAGATCAAGGGATTG+GGG 0.455280 1:+68569773 None:intergenic
ATTATATATACAGCCACTCT+TGG 0.456768 1:-68570059 MsG0180003811.01.T01:three_prime_UTR
ATTGGGAGAGATCAAGGGAT+TGG 0.457400 1:+68569771 None:intergenic
GAGTTTGAGTTTAATGTGAA+TGG 0.458393 1:+68571947 None:intergenic
CGCCGGAGGTTGTTCGCGGC+GGG 0.458516 1:+68571822 None:intergenic
AGAGAACATGATAAGGAGTC+TGG 0.464167 1:-68571491 MsG0180003811.01.T01:CDS
AAGATAGTAGCAGAGCATAT+TGG 0.466472 1:-68572088 MsG0180003811.01.T01:five_prime_UTR
CCTGATGCAGGTGTTGATCC+AGG 0.466664 1:+68570097 None:intergenic
GAAGCAATCCGAGCAGCAAC+CGG 0.468103 1:+68570640 None:intergenic
CGCCACAGTCAATAAAGATT+GGG 0.470327 1:+68569754 None:intergenic
ACCGGATCATTTGGTGGGAG+TGG 0.474405 1:+68570658 None:intergenic
GTGCTTGACATTAATCCTGA+TGG 0.479670 1:+68570807 None:intergenic
TCCACTCCCACCAAATGATC+CGG 0.488066 1:-68570659 MsG0180003811.01.T01:CDS
CGGAGTTAGGATCTTCTAAC+CGG 0.488892 1:+68571714 None:intergenic
TAACCGCCGGAGGTTGTTCG+CGG 0.489732 1:+68571818 None:intergenic
GCGTCGTATCTCGCCGTGAC+CGG 0.490086 1:-68571745 MsG0180003811.01.T01:CDS
TATCTCGACGAATCGAGAAC+CGG 0.491919 1:+68571635 None:intergenic
GTCCTGTATCTAGTGTTAAA+GGG 0.497916 1:-68570612 MsG0180003811.01.T01:CDS
CGGACCAGATCTTGTCTGAT+TGG 0.500982 1:+68571777 None:intergenic
ATCGACAGCTGCGTCAGGAT+TGG 0.502193 1:-68570036 MsG0180003811.01.T01:three_prime_UTR
GTTTATTGGATTGGGGTTTG+CGG 0.504982 1:-68571573 MsG0180003811.01.T01:CDS
ATACGAGTATCTTAACTAAG+AGG 0.506250 1:-68569996 MsG0180003811.01.T01:three_prime_UTR
CAATCAGACAAGATCTGGTC+CGG 0.507755 1:-68571776 MsG0180003811.01.T01:CDS
AGTGTTAAAGGGACAAAAGA+AGG 0.515401 1:-68570601 MsG0180003811.01.T01:CDS
ACCGGATCTCGCACCGGTCA+CGG 0.515952 1:+68571732 None:intergenic
AGATACGACGCGGAGACGGC+CGG 0.516487 1:+68571757 None:intergenic
AAGGCTGCCCATCCTGATGC+AGG 0.520170 1:+68570085 None:intergenic
GTCGTTAGAAGAAGAAGAAG+AGG 0.522046 1:-68571894 MsG0180003811.01.T01:CDS
TGATTGCTCGAGGAATGTCC+TGG 0.523604 1:-68569859 MsG0180003811.01.T01:three_prime_UTR
AATGTCAAGCACAAGGCGGC+TGG 0.531510 1:-68570796 MsG0180003811.01.T01:CDS
ACCGAACCCTCACGCTGACC+GGG 0.532951 1:+68571662 None:intergenic
TTGAAGGAAGCTGAAGAAAG+AGG 0.533212 1:-68569919 MsG0180003811.01.T01:three_prime_UTR
TGTAGGAGGGAGAAACCATC+AGG 0.535611 1:-68570822 MsG0180003811.01.T01:intron
TCTCCTAAAACAGTTGACCT+TGG 0.544438 1:-68572042 MsG0180003811.01.T01:five_prime_UTR
GTCAAGCACAAGGCGGCTGG+TGG 0.546548 1:-68570793 MsG0180003811.01.T01:CDS
CAAATGATCCGGTTGCTGCT+CGG 0.548131 1:-68570648 MsG0180003811.01.T01:CDS
CTGAAGATTGAAGATGGACA+AGG 0.551166 1:-68571605 MsG0180003811.01.T01:CDS
GCTGCTCGGATTGCTTCTAC+CGG 0.551616 1:-68570634 MsG0180003811.01.T01:CDS
AGCATGCGTTTATTGGATTG+GGG 0.552301 1:-68571580 MsG0180003811.01.T01:CDS
TTCGCCGCATTTGTAACCGC+CGG 0.553188 1:+68571805 None:intergenic
ACAGTCTACTGATTCTCTAT+CGG 0.561613 1:-68570572 MsG0180003811.01.T01:CDS
GTCATAAGTATAGTTAGCGC+AGG 0.564945 1:-68569895 MsG0180003811.01.T01:three_prime_UTR
CTCCCAATCTTTATTGACTG+TGG 0.565157 1:-68569756 MsG0180003811.01.T01:three_prime_UTR
GATCAACACCTGCATCAGGA+TGG 0.567922 1:-68570093 MsG0180003811.01.T01:CDS
TTGAGTAATTTGATTGCTCG+AGG 0.568756 1:-68569869 MsG0180003811.01.T01:three_prime_UTR
CGCAGCTGTCGATCCAAGAG+TGG 0.568796 1:+68570046 None:intergenic
TTGGATTGGGGTTTGCGGAG+AGG 0.569343 1:-68571568 MsG0180003811.01.T01:CDS
ATCAACACCTGCATCAGGAT+GGG 0.573253 1:-68570092 MsG0180003811.01.T01:CDS
CTTCTAACCGGATCTCGCAC+CGG 0.574822 1:+68571726 None:intergenic
AATGTATTTGTCTCAACTGT+GGG 0.581966 1:-68569671 MsG0180003811.01.T01:three_prime_UTR
CCGCCGGAGGTTGTTCGCGG+CGG 0.582317 1:+68571821 None:intergenic
GGCGAGATACGACGCGGAGA+CGG 0.582763 1:+68571753 None:intergenic
GCAGCAACCGGATCATTTGG+TGG 0.583616 1:+68570652 None:intergenic
GCGGTTACAAATGCGGCGAA+TGG 0.584085 1:-68571802 MsG0180003811.01.T01:CDS
TTGCTACATGCACACCCGAT+AGG 0.586697 1:+68570746 None:intergenic
TCGCGGCGGGATCTTGTAGA+CGG 0.592219 1:+68571835 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.596884 1:+68571808 None:intergenic
TTAAGCCTATGATTAACAGC+AGG 0.600310 1:+68571428 None:intergenic
TGGCTGTATATATAATCAGA+AGG 0.603345 1:+68570066 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.604789 1:-68571809 MsG0180003811.01.T01:CDS
TACTCGTATATATAATCAGA+AGG 0.605799 1:+68570010 None:intergenic
TGATCCAGGAGTGGCTGATG+AGG 0.607039 1:+68570111 None:intergenic
GTCTGGTGATGCTGATGCCG+CGG 0.607238 1:-68571474 MsG0180003811.01.T01:CDS
AAATGTATTTGTCTCAACTG+TGG 0.610206 1:-68569672 MsG0180003811.01.T01:three_prime_UTR
CGCGAACGACGAGGAGTGTG+TGG 0.610612 1:+68571864 None:intergenic
CAGCAACCGGATCATTTGGT+GGG 0.614621 1:+68570653 None:intergenic
CAGGATTAATGTCAAGCACA+AGG 0.616170 1:-68570803 MsG0180003811.01.T01:CDS
AAACATGTTATTACAAACCA+AGG 0.618335 1:+68569820 None:intergenic
TAAAGATTGGGAGAGATCAA+GGG 0.622275 1:+68569766 None:intergenic
TGAGAAACGCGTTATTGGTG+TGG 0.623056 1:+68571979 None:intergenic
CGGTTAGAAGATCCTAACTC+CGG 0.634275 1:-68571713 MsG0180003811.01.T01:CDS
ACACGCAGCGAAGAGATCAC+CGG 0.637569 1:+68571694 None:intergenic
TTTAACACTAGATACAGGAC+CGG 0.645020 1:+68570615 None:intergenic
CGGCGACTTCGCGAACGACG+AGG 0.648082 1:+68571855 None:intergenic
ATTTGGTGGGAGTGGAAGAG+CGG 0.652140 1:+68570666 None:intergenic
TGCAGGTGTTGATCCAGGAG+TGG 0.652617 1:+68570102 None:intergenic
TGAAGATTGAAGATGGACAA+GGG 0.659170 1:-68571604 MsG0180003811.01.T01:CDS
GATCCAGGAGTGGCTGATGA+GGG 0.665742 1:+68570112 None:intergenic
TGGTGATGCTGATGCCGCGG+AGG 0.679687 1:-68571471 MsG0180003811.01.T01:CDS
CCGCCGCGAACAACCTCCGG+CGG 0.687466 1:-68571821 MsG0180003811.01.T01:CDS
ATCGATCTGTGAATCCTCCG+CGG 0.729159 1:+68571457 None:intergenic
GATTAATGTCAAGCACAAGG+CGG 0.745005 1:-68570800 MsG0180003811.01.T01:CDS
CAAACACAAAGACAACACCA+AGG 0.791676 1:+68572025 None:intergenic
GGTCACGGCGAGATACGACG+CGG 0.826251 1:+68571747 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAGTTTTTTGTGTATTTT+TGG - Chr1:68570452-68570471 MsG0180003811.01.T01:intron 15.0%
!!! ATTATTCTCTGATTTATTTT+GGG - Chr1:68571738-68571757 MsG0180003811.01.T01:CDS 15.0%
!!! TATTATTCTCTGATTTATTT+TGG - Chr1:68571737-68571756 MsG0180003811.01.T01:CDS 15.0%
!! ACAATATAGAGAAATTAAAC+TGG + Chr1:68571505-68571524 None:intergenic 20.0%
!! TTATGATATAATGATGATCA+TGG - Chr1:68570537-68570556 MsG0180003811.01.T01:intron 20.0%
!! TTTGAGAAAAGAAATATTGA+AGG - Chr1:68571771-68571790 MsG0180003811.01.T01:CDS 20.0%
!!! GTAAACATGGTTTTAAAAAA+GGG + Chr1:68570777-68570796 None:intergenic 20.0%
!!! TGAACTTTAATTTATTGTAG+CGG - Chr1:68570356-68570375 MsG0180003811.01.T01:intron 20.0%
!!! TGTAAACATGGTTTTAAAAA+AGG + Chr1:68570778-68570797 None:intergenic 20.0%
!!! TTATTCTCTGATTTATTTTG+GGG - Chr1:68571739-68571758 MsG0180003811.01.T01:CDS 20.0%
! AAAACAAATGAAGTGGAAAT+AGG + Chr1:68571211-68571230 None:intergenic 25.0%
! AAAACCATGTTTACAAATCA+CGG - Chr1:68570783-68570802 MsG0180003811.01.T01:CDS 25.0%
! AAACATGTTATTACAAACCA+AGG + Chr1:68571889-68571908 None:intergenic 25.0%
! ATTCATATTAAACAGTGTCT+GGG + Chr1:68571534-68571553 None:intergenic 25.0%
! TACTCGTATATATAATCAGA+AGG + Chr1:68571699-68571718 None:intergenic 25.0%
! TATATGCATAAACAAAGCAA+TGG + Chr1:68571414-68571433 None:intergenic 25.0%
! TTGTAAAACAACAGTTACAT+TGG - Chr1:68571450-68571469 MsG0180003811.01.T01:CDS 25.0%
!! ACAACCATGATTTAAAACAT+TGG + Chr1:68570633-68570652 None:intergenic 25.0%
!! GAAATATTGATGTTTTTCCT+TGG - Chr1:68571869-68571888 MsG0180003811.01.T01:CDS 25.0%
!!! AATGTTTTAAATCATGGTTG+TGG - Chr1:68570632-68570651 MsG0180003811.01.T01:CDS 25.0%
!!! TATTCTCTGATTTATTTTGG+GGG - Chr1:68571740-68571759 MsG0180003811.01.T01:CDS 25.0%
!!! TTCTCCAATGTTTTAAATCA+TGG - Chr1:68570626-68570645 MsG0180003811.01.T01:CDS 25.0%
AAATGAAGTGGAAATAGGAA+AGG + Chr1:68571206-68571225 None:intergenic 30.0%
AAATGTATTTGTCTCAACTG+TGG - Chr1:68572034-68572053 MsG0180003811.01.T01:five_prime_UTR 30.0%
AATGTATTTGTCTCAACTGT+GGG - Chr1:68572035-68572054 MsG0180003811.01.T01:five_prime_UTR 30.0%
ACAGTTGAGACAAATACATT+TGG + Chr1:68572035-68572054 None:intergenic 30.0%
AGAACTTGCAAATTATTGCA+TGG - Chr1:68571247-68571266 MsG0180003811.01.T01:intron 30.0%
AGCATAGCATAATCTTAGAT+AGG + Chr1:68571347-68571366 None:intergenic 30.0%
AGTCACTATTGAGATTACAT+TGG + Chr1:68570704-68570723 None:intergenic 30.0%
ATACGAGTATCTTAACTAAG+AGG - Chr1:68571710-68571729 MsG0180003811.01.T01:CDS 30.0%
ATTATATATACAGCCACTCT+TGG - Chr1:68571647-68571666 MsG0180003811.01.T01:CDS 30.0%
ATTTGGGCAAAAAATAACCT+CGG + Chr1:68570571-68570590 None:intergenic 30.0%
CAGTTGAGACAAATACATTT+GGG + Chr1:68572034-68572053 None:intergenic 30.0%
CATAATCTTAGATAGGTACA+CGG + Chr1:68571340-68571359 None:intergenic 30.0%
CTAGAAACTACTCTAAACAT+CGG + Chr1:68570594-68570613 None:intergenic 30.0%
GATTCATATTAAACAGTGTC+TGG + Chr1:68571535-68571554 None:intergenic 30.0%
GATTGCAAAAACAAATGAAG+TGG + Chr1:68571218-68571237 None:intergenic 30.0%
GGAAAAACATCAATATTTCC+AGG + Chr1:68571868-68571887 None:intergenic 30.0%
TCGACAAATACTTCAAACAT+AGG + Chr1:68570858-68570877 None:intergenic 30.0%
TGGCTGTATATATAATCAGA+AGG + Chr1:68571643-68571662 None:intergenic 30.0%
TTGGTTAGTGTTAATTGTTG+TGG - Chr1:68570471-68570490 MsG0180003811.01.T01:intron 30.0%
! CTTATGATTTTTGTGCTCAA+AGG - Chr1:68571977-68571996 MsG0180003811.01.T01:five_prime_UTR 30.0%
! GAGTTTGAGTTTAATGTGAA+TGG + Chr1:68569762-68569781 None:intergenic 30.0%
! TGTTTGAAGTATTTGTCGAT+TGG - Chr1:68570859-68570878 MsG0180003811.01.T01:intron 30.0%
!! TGATCACACAAATTTGCTAA+TGG + Chr1:68570312-68570331 None:intergenic 30.0%
!! TTTGTTCTGAAGATTGAAGA+TGG - Chr1:68570095-68570114 MsG0180003811.01.T01:CDS 30.0%
AAGATAGTAGCAGAGCATAT+TGG - Chr1:68569618-68569637 MsG0180003811.01.T01:three_prime_UTR 35.0%
AAGCATGCGTTTATTGGATT+GGG - Chr1:68570125-68570144 MsG0180003811.01.T01:intron 35.0%
ACTACTCTAAACATCGGATT+TGG + Chr1:68570588-68570607 None:intergenic 35.0%
AGTGTTAAAGGGACAAAAGA+AGG - Chr1:68571105-68571124 MsG0180003811.01.T01:intron 35.0%
ATAAAGATTGGGAGAGATCA+AGG + Chr1:68571944-68571963 None:intergenic 35.0%
ATCGGATCTTTCTCAATTAC+AGG - Chr1:68571152-68571171 MsG0180003811.01.T01:intron 35.0%
CTACTCTAAACATCGGATTT+GGG + Chr1:68570587-68570606 None:intergenic 35.0%
CTTCAAACATAGGATAAGCA+AGG + Chr1:68570848-68570867 None:intergenic 35.0%
GAATTTGAGAAACGCGTTAT+TGG + Chr1:68569735-68569754 None:intergenic 35.0%
GTCCTGTATCTAGTGTTAAA+GGG - Chr1:68571094-68571113 MsG0180003811.01.T01:intron 35.0%
TAAAAAAGGGTTTGCGATCT+GGG + Chr1:68570764-68570783 None:intergenic 35.0%
TAAAGATTGGGAGAGATCAA+GGG + Chr1:68571943-68571962 None:intergenic 35.0%
TCGTTAGAAGAAGAAGAAGA+GGG - Chr1:68569813-68569832 MsG0180003811.01.T01:three_prime_UTR 35.0%
TGAAGATTGAAGATGGACAA+GGG - Chr1:68570102-68570121 MsG0180003811.01.T01:CDS 35.0%
TGCTGTTAATCATAGGCTTA+AGG - Chr1:68570280-68570299 MsG0180003811.01.T01:intron 35.0%
TGTGAGAAGAGAACATGATA+AGG - Chr1:68570208-68570227 MsG0180003811.01.T01:intron 35.0%
TTAAAAAAGGGTTTGCGATC+TGG + Chr1:68570765-68570784 None:intergenic 35.0%
TTAAGCCTATGATTAACAGC+AGG + Chr1:68570281-68570300 None:intergenic 35.0%
TTCATCCTGCTGTTAATCAT+AGG - Chr1:68570273-68570292 MsG0180003811.01.T01:intron 35.0%
TTGAGTAATTTGATTGCTCG+AGG - Chr1:68571837-68571856 MsG0180003811.01.T01:CDS 35.0%
TTGCAAATTATTGCATGGCT+GGG - Chr1:68571252-68571271 MsG0180003811.01.T01:intron 35.0%
TTTAACACTAGATACAGGAC+CGG + Chr1:68571094-68571113 None:intergenic 35.0%
TTTCTCAATTACAGGTATGC+TGG - Chr1:68571160-68571179 MsG0180003811.01.T01:intron 35.0%
! AAGACCGTGATTTGTAAACA+TGG + Chr1:68570790-68570809 None:intergenic 35.0%
! TTCTTCTAACGACATCGTTT+TGG + Chr1:68569805-68569824 None:intergenic 35.0%
! TTGCTATTGCAAATGATCGT+GGG - Chr1:68570669-68570688 MsG0180003811.01.T01:CDS 35.0%
!! ACAGTCTACTGATTCTCTAT+CGG - Chr1:68571134-68571153 MsG0180003811.01.T01:intron 35.0%
!! GACAAATACATTTGGGTTGA+TGG + Chr1:68572027-68572046 None:intergenic 35.0%
!!! AGGTTCAATACTTTTGGCTT+CGG + Chr1:68570988-68571007 None:intergenic 35.0%
ACGCCACAGTCAATAAAGAT+TGG + Chr1:68571956-68571975 None:intergenic 40.0%
AGAGAACATGATAAGGAGTC+TGG - Chr1:68570215-68570234 MsG0180003811.01.T01:intron 40.0%
AGCATGCGTTTATTGGATTG+GGG - Chr1:68570126-68570145 MsG0180003811.01.T01:intron 40.0%
CAAACACAAAGACAACACCA+AGG + Chr1:68569684-68569703 None:intergenic 40.0%
CAGGATTAATGTCAAGCACA+AGG - Chr1:68570903-68570922 MsG0180003811.01.T01:intron 40.0%
CGCCACAGTCAATAAAGATT+GGG + Chr1:68571955-68571974 None:intergenic 40.0%
CTGAAGATTGAAGATGGACA+AGG - Chr1:68570101-68570120 MsG0180003811.01.T01:CDS 40.0%
CTGGCAAAGTAACATGATCA+CGG + Chr1:68571486-68571505 None:intergenic 40.0%
CTTGCAAATTATTGCATGGC+TGG - Chr1:68571251-68571270 MsG0180003811.01.T01:intron 40.0%
GAAGCATGCGTTTATTGGAT+TGG - Chr1:68570124-68570143 MsG0180003811.01.T01:CDS 40.0%
GATTAATGTCAAGCACAAGG+CGG - Chr1:68570906-68570925 MsG0180003811.01.T01:intron 40.0%
GGAAATAGGAAAGGCTCTTA+AGG + Chr1:68571197-68571216 None:intergenic 40.0%
GGTCCTGTATCTAGTGTTAA+AGG - Chr1:68571093-68571112 MsG0180003811.01.T01:intron 40.0%
GTCATAAGTATAGTTAGCGC+AGG - Chr1:68571811-68571830 MsG0180003811.01.T01:CDS 40.0%
GTCCCTTTAACACTAGATAC+AGG + Chr1:68571099-68571118 None:intergenic 40.0%
GTCGTTAGAAGAAGAAGAAG+AGG - Chr1:68569812-68569831 MsG0180003811.01.T01:three_prime_UTR 40.0%
GTGCTTGACATTAATCCTGA+TGG + Chr1:68570902-68570921 None:intergenic 40.0%
TCTCCTAAAACAGTTGACCT+TGG - Chr1:68569664-68569683 MsG0180003811.01.T01:three_prime_UTR 40.0%
TTGAAGGAAGCTGAAGAAAG+AGG - Chr1:68571787-68571806 MsG0180003811.01.T01:CDS 40.0%
! ACACCAAGGTCAACTGTTTT+AGG + Chr1:68569670-68569689 None:intergenic 40.0%
! CTCCCAATCTTTATTGACTG+TGG - Chr1:68571950-68571969 MsG0180003811.01.T01:five_prime_UTR 40.0%
! GGAGCAAGGTTCAATACTTT+TGG + Chr1:68570994-68571013 None:intergenic 40.0%
! GTTGCTATTGCAAATGATCG+TGG - Chr1:68570668-68570687 MsG0180003811.01.T01:CDS 40.0%
! GTTTATTGGATTGGGGTTTG+CGG - Chr1:68570133-68570152 MsG0180003811.01.T01:intron 40.0%
AAGGCTCTTAAGGAATGAGC+AGG + Chr1:68571187-68571206 None:intergenic 45.0%
ATCAACACCTGCATCAGGAT+GGG - Chr1:68571614-68571633 MsG0180003811.01.T01:CDS 45.0%
ATTGGGAGAGATCAAGGGAT+TGG + Chr1:68571938-68571957 None:intergenic 45.0%
CAAATGATCGTGGGCAAATG+GGG - Chr1:68570678-68570697 MsG0180003811.01.T01:CDS 45.0%
CAATCAGACAAGATCTGGTC+CGG - Chr1:68569930-68569949 MsG0180003811.01.T01:three_prime_UTR 45.0%
CGGAGTTAGGATCTTCTAAC+CGG + Chr1:68569995-68570014 None:intergenic 45.0%
CGGTTAGAAGATCCTAACTC+CGG - Chr1:68569993-68570012 MsG0180003811.01.T01:three_prime_UTR 45.0%
GCAAATGATCGTGGGCAAAT+GGG - Chr1:68570677-68570696 MsG0180003811.01.T01:CDS 45.0%
TATCTCGACGAATCGAGAAC+CGG + Chr1:68570074-68570093 None:intergenic 45.0%
TGAGAAACGCGTTATTGGTG+TGG + Chr1:68569730-68569749 None:intergenic 45.0%
TGATCATGGTGAATTGTCCG+AGG - Chr1:68570551-68570570 MsG0180003811.01.T01:intron 45.0%
TGCAAATGATCGTGGGCAAA+TGG - Chr1:68570676-68570695 MsG0180003811.01.T01:CDS 45.0%
TTGGGAGAGATCAAGGGATT+GGG + Chr1:68571937-68571956 None:intergenic 45.0%
! CAAGGGAAGCATGCGTTTAT+TGG - Chr1:68570119-68570138 MsG0180003811.01.T01:CDS 45.0%
!! GTATTTGTCGATTGGCCTGT+AGG - Chr1:68570867-68570886 MsG0180003811.01.T01:intron 45.0%
!!! GCTGCGTGTTTTGTTTTTCC+CGG - Chr1:68570026-68570045 MsG0180003811.01.T01:three_prime_UTR 45.0%
!! AAAATAAAAAATAAAAATAC+AGG - Chr1:68572093-68572112 MsG0180003811.01.T01:five_prime_UTR 5.0%
ATCGATCTGTGAATCCTCCG+CGG + Chr1:68570252-68570271 None:intergenic 50.0%
CAAATGATCCGGTTGCTGCT+CGG - Chr1:68571058-68571077 MsG0180003811.01.T01:intron 50.0%
CAGCAACCGGATCATTTGGT+GGG + Chr1:68571056-68571075 None:intergenic 50.0%
CGATCTGGGTTGCAACATCA+AGG + Chr1:68570750-68570769 None:intergenic 50.0%
CGGACCAGATCTTGTCTGAT+TGG + Chr1:68569932-68569951 None:intergenic 50.0%
GATCAACACCTGCATCAGGA+TGG - Chr1:68571613-68571632 MsG0180003811.01.T01:CDS 50.0%
GGCTCCAATCAGACAAGATC+TGG - Chr1:68569925-68569944 MsG0180003811.01.T01:three_prime_UTR 50.0%
TCCACTCCCACCAAATGATC+CGG - Chr1:68571047-68571066 MsG0180003811.01.T01:intron 50.0%
TGATGGTTTCTCCCTCCTAC+AGG + Chr1:68570885-68570904 None:intergenic 50.0%
TGATTGCTCGAGGAATGTCC+TGG - Chr1:68571847-68571866 MsG0180003811.01.T01:CDS 50.0%
TGGGAGAGATCAAGGGATTG+GGG + Chr1:68571936-68571955 None:intergenic 50.0%
TGTAGGAGGGAGAAACCATC+AGG - Chr1:68570884-68570903 MsG0180003811.01.T01:intron 50.0%
TTGCTACATGCACACCCGAT+AGG + Chr1:68570963-68570982 None:intergenic 50.0%
! ATTTGGTGGGAGTGGAAGAG+CGG + Chr1:68571043-68571062 None:intergenic 50.0%
! GATCTGATTTTCCCTGCACC+CGG + Chr1:68571021-68571040 None:intergenic 50.0%
!! TTGTCGATTGGCCTGTAGGA+GGG - Chr1:68570871-68570890 MsG0180003811.01.T01:intron 50.0%
!! TTTGTCGATTGGCCTGTAGG+AGG - Chr1:68570870-68570889 MsG0180003811.01.T01:intron 50.0%
AATGTCAAGCACAAGGCGGC+TGG - Chr1:68570910-68570929 MsG0180003811.01.T01:intron 55.0%
ACACGCAGCGAAGAGATCAC+CGG + Chr1:68570015-68570034 None:intergenic 55.0%
ACCTCCGGCGGTTACAAATG+CGG - Chr1:68569897-68569916 MsG0180003811.01.T01:three_prime_UTR 55.0%
ATCGACAGCTGCGTCAGGAT+TGG - Chr1:68571670-68571689 MsG0180003811.01.T01:CDS 55.0%
ATTGAACCTTGCTCCCCCAC+CGG - Chr1:68570999-68571018 MsG0180003811.01.T01:intron 55.0%
CCTGGATCAACACCTGCATC+AGG - Chr1:68571609-68571628 MsG0180003811.01.T01:CDS 55.0%
CGAGCAGCAACCGGATCATT+TGG + Chr1:68571060-68571079 None:intergenic 55.0%
CTTCTAACCGGATCTCGCAC+CGG + Chr1:68569983-68570002 None:intergenic 55.0%
GAAGCAATCCGAGCAGCAAC+CGG + Chr1:68571069-68571088 None:intergenic 55.0%
GATCCAGGAGTGGCTGATGA+GGG + Chr1:68571597-68571616 None:intergenic 55.0%
GCAGCAACCGGATCATTTGG+TGG + Chr1:68571057-68571076 None:intergenic 55.0%
GCGAAGAGATCACCGGAGTT+AGG + Chr1:68570008-68570027 None:intergenic 55.0%
GCGGTTACAAATGCGGCGAA+TGG - Chr1:68569904-68569923 MsG0180003811.01.T01:three_prime_UTR 55.0%
GCTGCTCGGATTGCTTCTAC+CGG - Chr1:68571072-68571091 MsG0180003811.01.T01:intron 55.0%
TGATCCAGGAGTGGCTGATG+AGG + Chr1:68571598-68571617 None:intergenic 55.0%
TTCGCCGCATTTGTAACCGC+CGG + Chr1:68569904-68569923 None:intergenic 55.0%
! ACCGGATCATTTGGTGGGAG+TGG + Chr1:68571051-68571070 None:intergenic 55.0%
! CTTTCAGCTGCTGGCCTATC+GGG - Chr1:68570946-68570965 MsG0180003811.01.T01:intron 55.0%
! GCTTTCAGCTGCTGGCCTAT+CGG - Chr1:68570945-68570964 MsG0180003811.01.T01:intron 55.0%
! GTTTTTCCCGGTCAGCGTGA+GGG - Chr1:68570038-68570057 MsG0180003811.01.T01:three_prime_UTR 55.0%
! TGATTTTCCCTGCACCCGGT+GGG + Chr1:68571017-68571036 None:intergenic 55.0%
! TGTTTTTCCCGGTCAGCGTG+AGG - Chr1:68570037-68570056 MsG0180003811.01.T01:three_prime_UTR 55.0%
! TTGGATTGGGGTTTGCGGAG+AGG - Chr1:68570138-68570157 MsG0180003811.01.T01:intron 55.0%
!! CCTGATGCAGGTGTTGATCC+AGG + Chr1:68571612-68571631 None:intergenic 55.0%
!! TGCAGGTGTTGATCCAGGAG+TGG + Chr1:68571607-68571626 None:intergenic 55.0%
AACCGAACCCTCACGCTGAC+CGG + Chr1:68570048-68570067 None:intergenic 60.0%
AAGGCTGCCCATCCTGATGC+AGG + Chr1:68571624-68571643 None:intergenic 60.0%
CGCAGCTGTCGATCCAAGAG+TGG + Chr1:68571663-68571682 None:intergenic 60.0%
CTTCCCTCATCAGCCACTCC+TGG - Chr1:68571591-68571610 MsG0180003811.01.T01:CDS 60.0%
CTTGGATCGACAGCTGCGTC+AGG - Chr1:68571665-68571684 MsG0180003811.01.T01:CDS 60.0%
GCTGGCATGCTTTCAGCTGC+TGG - Chr1:68570937-68570956 MsG0180003811.01.T01:intron 60.0%
TAACCGCCGGAGGTTGTTCG+CGG + Chr1:68569891-68569910 None:intergenic 60.0%
TCGCGGCGGGATCTTGTAGA+CGG + Chr1:68569874-68569893 None:intergenic 60.0%
TTGAACCTTGCTCCCCCACC+GGG - Chr1:68571000-68571019 MsG0180003811.01.T01:intron 60.0%
! ATTTTCCCTGCACCCGGTGG+GGG + Chr1:68571015-68571034 None:intergenic 60.0%
! CTGATTTTCCCTGCACCCGG+TGG + Chr1:68571018-68571037 None:intergenic 60.0%
! GATTTTCCCTGCACCCGGTG+GGG + Chr1:68571016-68571035 None:intergenic 60.0%
! GCGTGAGGGTTCGGTTGAAC+CGG - Chr1:68570052-68570071 MsG0180003811.01.T01:three_prime_UTR 60.0%
! GTCTGGTGATGCTGATGCCG+CGG - Chr1:68570232-68570251 MsG0180003811.01.T01:intron 60.0%
ACCGAACCCTCACGCTGACC+GGG + Chr1:68570047-68570066 None:intergenic 65.0%
AGATACGACGCGGAGACGGC+CGG + Chr1:68569952-68569971 None:intergenic 65.0%
ATCCCGCCGCGAACAACCTC+CGG - Chr1:68569882-68569901 MsG0180003811.01.T01:three_prime_UTR 65.0%
GCCGCATTTGTAACCGCCGG+AGG + Chr1:68569901-68569920 None:intergenic 65.0%
GCGTCGTATCTCGCCGTGAC+CGG - Chr1:68569961-68569980 MsG0180003811.01.T01:three_prime_UTR 65.0%
GGCGAGATACGACGCGGAGA+CGG + Chr1:68569956-68569975 None:intergenic 65.0%
GGTCACGGCGAGATACGACG+CGG + Chr1:68569962-68569981 None:intergenic 65.0%
GTCAAGCACAAGGCGGCTGG+TGG - Chr1:68570913-68570932 MsG0180003811.01.T01:intron 65.0%
TCCCGGTCAGCGTGAGGGTT+CGG - Chr1:68570043-68570062 MsG0180003811.01.T01:three_prime_UTR 65.0%
! ACCGGATCTCGCACCGGTCA+CGG + Chr1:68569977-68569996 None:intergenic 65.0%
! CGCGAACGACGAGGAGTGTG+TGG + Chr1:68569845-68569864 None:intergenic 65.0%
! TGGTGATGCTGATGCCGCGG+AGG - Chr1:68570235-68570254 MsG0180003811.01.T01:intron 65.0%
CACAAGGCGGCTGGTGGAGC+TGG - Chr1:68570919-68570938 MsG0180003811.01.T01:intron 70.0%
CGGCGACTTCGCGAACGACG+AGG + Chr1:68569854-68569873 None:intergenic 70.0%
GCCGTGACCGGTGCGAGATC+CGG - Chr1:68569973-68569992 MsG0180003811.01.T01:three_prime_UTR 70.0%
TTGCTCCCCCACCGGGTGCA+GGG - Chr1:68571007-68571026 MsG0180003811.01.T01:intron 70.0%
CCGCCGCGAACAACCTCCGG+CGG - Chr1:68569885-68569904 MsG0180003811.01.T01:three_prime_UTR 75.0%
CCGCCGGAGGTTGTTCGCGG+CGG + Chr1:68569888-68569907 None:intergenic 75.0%
CGCCGGAGGTTGTTCGCGGC+GGG + Chr1:68569887-68569906 None:intergenic 75.0%
CTGCACCCGGTGGGGGAGCA+AGG + Chr1:68571008-68571027 None:intergenic 75.0%
CTTGCTCCCCCACCGGGTGC+AGG - Chr1:68571006-68571025 MsG0180003811.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 68569612 68572116 68569612 ID=MsG0180003811.01;Name=MsG0180003811.01
Chr1 mRNA 68569612 68572116 68569612 ID=MsG0180003811.01.T01;Parent=MsG0180003811.01;Name=MsG0180003811.01.T01;_AED=0.47;_eAED=0.47;_QI=198|1|1|1|1|1|3|469|279
Chr1 exon 68569612 68570143 68569612 ID=MsG0180003811.01.T01:exon:24284;Parent=MsG0180003811.01.T01
Chr1 exon 68570555 68570839 68570555 ID=MsG0180003811.01.T01:exon:24283;Parent=MsG0180003811.01.T01
Chr1 exon 68571427 68572116 68571427 ID=MsG0180003811.01.T01:exon:24282;Parent=MsG0180003811.01.T01
Chr1 five_prime_UTR 68571919 68572116 68571919 ID=MsG0180003811.01.T01:five_prime_utr;Parent=MsG0180003811.01.T01
Chr1 CDS 68571427 68571918 68571427 ID=MsG0180003811.01.T01:cds;Parent=MsG0180003811.01.T01
Chr1 CDS 68570555 68570839 68570555 ID=MsG0180003811.01.T01:cds;Parent=MsG0180003811.01.T01
Chr1 CDS 68570081 68570143 68570081 ID=MsG0180003811.01.T01:cds;Parent=MsG0180003811.01.T01
Chr1 three_prime_UTR 68569612 68570080 68569612 ID=MsG0180003811.01.T01:three_prime_utr;Parent=MsG0180003811.01.T01
Gene Sequence

>MsG0180003811.01.T01

ATGTCGTTAGAAGAAGAAGAAGAGGGCTTCGACCACACACTCCTCGTCGTTCGCGAAGTCGCCGTCTACAAGATCCCGCCGCGAACAACCTCCGGCGGTTACAAATGCGGCGAATGGCTCCAATCAGACAAGATCTGGTCCGGCCGTCTCCGCGTCGTATCTCGCCGTGACCGGTGCGAGATCCGGTTAGAAGATCCTAACTCCGGTGATCTCTTCGCTGCGTGTTTTGTTTTTCCCGGTCAGCGTGAGGGTTCGGTTGAACCGGTTCTCGATTCGTCGAGATATTTTGTTCTGAAGATTGAAGATGGACAAGGGAAGCATGCGTTTATTGGATTGGGGTTTGCGGAGAGGAATGAAGCTTTTGATTTCAATGTTGCACTTTCTGATCATGAGAAGTATGTGAGAAGAGAACATGATAAGGAGTCTGGTGATGCTGATGCCGCGGAGGATTCACAGATCGATATTCATCCTGCTGTTAATCATAGGCTTAAGGAGGGAGAAACCATCAGGATTAATGTCAAGCACAAGGCGGCTGGTGGAGCTGGCATGCTTTCAGCTGCTGGCCTATCGGGTGTGCATGTAGCAACACCGAAGCCAAAAGTATTGAACCTTGCTCCCCCACCGGGTGCAGGGAAAATCAGATCCGCTCTTCCACTCCCACCAAATGATCCGGTTGCTGCTCGGATTGCTTCTACCGGTCCTGTATCTAGTGTTAAAGGGACAAAAGAAGGTGTGAAACAGTCTACTGATTCTCTATCGGATCTTTCTCAATTACAGAAAAATCTTCCCTCATCAGCCACTCCTGGATCAACACCTGCATCAGGATGGGCAGCCTTCTGA
Protein sequence

>MsG0180003811.01.T01

MSLEEEEEGFDHTLLVVREVAVYKIPPRTTSGGYKCGEWLQSDKIWSGRLRVVSRRDRCEIRLEDPNSGDLFAACFVFPGQREGSVEPVLDSSRYFVLKIEDGQGKHAFIGLGFAERNEAFDFNVALSDHEKYVRREHDKESGDADAAEDSQIDIHPAVNHRLKEGETIRINVKHKAAGGAGMLSAAGLSGVHVATPKPKVLNLAPPPGAGKIRSALPLPPNDPVAARIASTGPVSSVKGTKEGVKQSTDSLSDLSQLQKNLPSSATPGSTPASGWAAF*