AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003944.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003944.01.T01 MTR_1g071470 93.253 415 28 0 1 415 1 415 0.0 791
MsG0180003944.01.T01 MTR_0314s0030 26.531 343 167 10 29 338 25 315 3.49e-19 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003944.01.T01 AT3G63290 48.416 442 162 7 1 415 1 403 1.03e-128 377
MsG0180003944.01.T01 AT3G63290 50.318 314 111 5 108 415 29 303 2.65e-98 296

Find 107 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAAACAAATGCTAATTT+TGG 0.071672 1:-70710522 MsG0180003944.01.T01:three_prime_UTR
AAAGATAAAGATCAAGTTTA+TGG 0.165385 1:-70711568 MsG0180003944.01.T01:CDS
CCCACTTTCATCGCGGTTAA+AGG 0.171080 1:-70710750 MsG0180003944.01.T01:CDS
ACTTGAACAATTAAACTTTC+AGG 0.246183 1:+70711052 None:intergenic
TGAATTGGAGCAGAGTTTAT+TGG 0.247283 1:-70711440 MsG0180003944.01.T01:CDS
CATTATGGAATCTTCTACTT+TGG 0.250206 1:+70712109 None:intergenic
ATTTGATACGTTGGGGATTC+CGG 0.291583 1:+70712046 None:intergenic
ACCCAAAATTAGCATTTGTT+TGG 0.314844 1:+70710520 None:intergenic
TGAGGATGCGATTACGAGTT+TGG 0.321530 1:+70711973 None:intergenic
AAAGTTTAATTGTTCAAGTT+GGG 0.325298 1:-70711048 MsG0180003944.01.T01:CDS
CAGCAGTGGCATTATGATTA+TGG 0.338813 1:-70711241 MsG0180003944.01.T01:CDS
CTTCTTGTCAATTGTGGGTT+AGG 0.339564 1:-70710562 MsG0180003944.01.T01:three_prime_UTR
GGATCCGAGTGCTTCCATTA+TGG 0.343808 1:+70712094 None:intergenic
AAAGATCAAGTTTATGGTAA+TGG 0.356663 1:-70711562 MsG0180003944.01.T01:CDS
GGCTATTGGTGGAAATGAAT+TGG 0.359922 1:-70711455 MsG0180003944.01.T01:CDS
AAGAATGTGAAGCCATTAGA+AGG 0.367923 1:-70711313 MsG0180003944.01.T01:CDS
GTTGAATGTCCATCGCCAAC+TGG 0.374226 1:-70711136 MsG0180003944.01.T01:CDS
TGCTTGTGATGCAGTTCATT+CGG 0.387308 1:-70711272 MsG0180003944.01.T01:CDS
TCTATCTCGGATTATCCTCT+TGG 0.388299 1:-70710893 MsG0180003944.01.T01:CDS
GGTAAGACAAATAAGAAAAG+AGG 0.390928 1:-70711340 MsG0180003944.01.T01:CDS
GGTAGATGGTCAGTGTTTAA+TGG 0.391286 1:-70710852 MsG0180003944.01.T01:CDS
TTCAAACACGACCCTTCTAA+TGG 0.403945 1:+70711301 None:intergenic
TAGATGACTAAGTACTCGTT+TGG 0.406326 1:-70710654 MsG0180003944.01.T01:exon
CTGCTTCAAGAATTTGATAA+GGG 0.407840 1:-70711361 MsG0180003944.01.T01:CDS
GAAACAACAAAGCAGTATTA+TGG 0.410947 1:-70710698 MsG0180003944.01.T01:CDS
CCGGTGACGGCGAGGAGGCC+TGG 0.411424 1:+70712065 None:intergenic
AATTGTTCAAGTTGGGGAAT+CGG 0.415016 1:-70711041 MsG0180003944.01.T01:CDS
TACTAGCTTCTTGTCAATTG+TGG 0.417976 1:-70710568 MsG0180003944.01.T01:three_prime_UTR
GAGCGGAGATTTGATACGTT+GGG 0.426675 1:+70712038 None:intergenic
ACTAGCTTCTTGTCAATTGT+GGG 0.430186 1:-70710567 MsG0180003944.01.T01:three_prime_UTR
TCGACATTCGATGGGGTAGA+TGG 0.430230 1:-70710866 MsG0180003944.01.T01:CDS
GGTAATGGATTTGAGAATCT+AGG 0.432228 1:-70711547 MsG0180003944.01.T01:CDS
TTGGAATCACTTGCTGCAAA+GGG 0.434320 1:-70711421 MsG0180003944.01.T01:CDS
AGAATTTGTGATAAGGCTAT+TGG 0.439241 1:-70711469 MsG0180003944.01.T01:CDS
TATATGATCCTAATAAGAAA+AGG 0.445854 1:-70711096 MsG0180003944.01.T01:CDS
TCTGCTTCAAGAATTTGATA+AGG 0.446474 1:-70711362 MsG0180003944.01.T01:CDS
GAAAGTTTAATTGTTCAAGT+TGG 0.449188 1:-70711049 MsG0180003944.01.T01:CDS
GCTTGCTAGAATTTGTGATA+AGG 0.453159 1:-70711476 MsG0180003944.01.T01:CDS
TTGTATTTCTGCAGCCTGCA+TGG 0.457680 1:-70710928 MsG0180003944.01.T01:CDS
GTAAGACAAATAAGAAAAGA+GGG 0.460920 1:-70711339 MsG0180003944.01.T01:CDS
CCAGGCCTCCTCGCCGTCAC+CGG 0.462208 1:-70712065 MsG0180003944.01.T01:CDS
GGCGAGGAGGCCTGGACCGT+TGG 0.467952 1:+70712073 None:intergenic
GGGAGTACATTGACTGATAC+AGG 0.469549 1:+70710493 None:intergenic
AAGGTCTATAAACAGAATGT+AGG 0.471680 1:+70710984 None:intergenic
GACAAGTACAATGCAAGATT+CGG 0.478973 1:-70711167 MsG0180003944.01.T01:CDS
CAAAGTAGAAGATTCCATAA+TGG 0.479822 1:-70712108 MsG0180003944.01.T01:CDS
TAATAAGAAAAGGGTAGTTA+TGG 0.482400 1:-70711086 MsG0180003944.01.T01:CDS
CTTTCATCGCGGTTAAAGGA+TGG 0.489406 1:-70710746 MsG0180003944.01.T01:CDS
TACGTTGGGGATTCCGGTGA+CGG 0.489634 1:+70712052 None:intergenic
TCCTTTAACCGCGATGAAAG+TGG 0.496725 1:+70710749 None:intergenic
CCTTTAACCGCGATGAAAGT+GGG 0.502673 1:+70710750 None:intergenic
TGGTATATTTACTGTTCTAA+CGG 0.502966 1:-70711221 MsG0180003944.01.T01:CDS
AAGCAGTATTATGGTGGTAG+TGG 0.503621 1:-70710689 MsG0180003944.01.T01:CDS
ATGCAGTTCATTCGGATTTG+TGG 0.507679 1:-70711264 MsG0180003944.01.T01:CDS
ATTTGTGATAAGGCTATTGG+TGG 0.511639 1:-70711466 MsG0180003944.01.T01:CDS
AAGTCTTATTGCTTTGGCAA+GGG 0.517927 1:+70710472 None:intergenic
GCACTCGGATCCAACGGTCC+AGG 0.518328 1:-70712083 MsG0180003944.01.T01:CDS
TGCAAATTCGTAAGCCCAGT+TGG 0.518561 1:+70711121 None:intergenic
GACTAAAACATTCTCTATCT+CGG 0.519771 1:-70710906 MsG0180003944.01.T01:CDS
AAAGTCTTATTGCTTTGGCA+AGG 0.521737 1:+70710471 None:intergenic
ATTGGAATCACTTGCTGCAA+AGG 0.525490 1:-70711422 MsG0180003944.01.T01:CDS
TTCGTTTGGAATTGAGTCGC+GGG 0.537331 1:+70710610 None:intergenic
TTCGTAAGCCCAGTTGGCGA+TGG 0.540587 1:+70711127 None:intergenic
ATATGATCCTAATAAGAAAA+GGG 0.541044 1:-70711095 MsG0180003944.01.T01:CDS
AGTCTTATTGCTTTGGCAAG+GGG 0.541087 1:+70710473 None:intergenic
CTTGGAAAATCGACATTCGA+TGG 0.546678 1:-70710875 MsG0180003944.01.T01:CDS
ATTCGGATTTGTGGCAGCAG+TGG 0.553217 1:-70711255 MsG0180003944.01.T01:CDS
TTGGAAAATCGACATTCGAT+GGG 0.557196 1:-70710874 MsG0180003944.01.T01:CDS
CGGCTGATATAATCTCGAAA+GGG 0.558251 1:-70711021 MsG0180003944.01.T01:CDS
TTTCATCGCGGTTAAAGGAT+GGG 0.560059 1:-70710745 MsG0180003944.01.T01:CDS
GCTTACGAGCGAGAGGGAGA+AGG 0.560525 1:+70712012 None:intergenic
TCGGCTGATATAATCTCGAA+AGG 0.561055 1:-70711022 MsG0180003944.01.T01:CDS
AAGTTTAATTGTTCAAGTTG+GGG 0.564180 1:-70711047 MsG0180003944.01.T01:CDS
TTCTAGGAGCATAACTTAGG+TGG 0.567203 1:-70711673 MsG0180003944.01.T01:intron
AGGTCTATAAACAGAATGTA+GGG 0.570615 1:+70710985 None:intergenic
AGCGGAGATTTGATACGTTG+GGG 0.571155 1:+70712039 None:intergenic
TGAAGAACAACACTCTCGAC+AGG 0.581501 1:-70710813 MsG0180003944.01.T01:CDS
TAATCTCGAAAGGGAAGCTA+CGG 0.597076 1:-70711012 MsG0180003944.01.T01:CDS
TTATCATTCTCGTTTAGATG+CGG 0.598987 1:-70711386 MsG0180003944.01.T01:CDS
AACTCGTAATCGCATCCTCA+AGG 0.599869 1:-70711970 MsG0180003944.01.T01:intron
TGAACAATTAAACTTTCAGG+AGG 0.600016 1:+70711055 None:intergenic
TGAGCGGAGATTTGATACGT+TGG 0.610128 1:+70712037 None:intergenic
TAAGACAAATAAGAAAAGAG+GGG 0.612032 1:-70711338 MsG0180003944.01.T01:CDS
ATGGAAGCACTCGGATCCAA+CGG 0.614436 1:-70712089 MsG0180003944.01.T01:CDS
TGGGGATTCCGGTGACGGCG+AGG 0.616485 1:+70712057 None:intergenic
CTTTAACCGCGATGAAAGTG+GGG 0.619284 1:+70710751 None:intergenic
TTGAATGTCCATCGCCAACT+GGG 0.619370 1:-70711135 MsG0180003944.01.T01:CDS
TGCGAAAATGGAAGAATGTG+AGG 0.620324 1:-70712183 None:intergenic
GTACTTGTCTCCGAATAAGA+CGG 0.622980 1:+70711181 None:intergenic
GAAAAGGGTAGTTATGGCGA+GGG 0.628421 1:-70711080 MsG0180003944.01.T01:CDS
TAAGACGGCAAAAGAAAACA+TGG 0.630871 1:+70711196 None:intergenic
AGAGCGAGCTTACGAGCGAG+AGG 0.631305 1:+70712005 None:intergenic
CGAGAGGGAGAAGGTATGAG+CGG 0.633375 1:+70712021 None:intergenic
AGAATGTGAAGCCATTAGAA+GGG 0.633475 1:-70711312 MsG0180003944.01.T01:CDS
GGATTCCGGTGACGGCGAGG+AGG 0.633957 1:+70712060 None:intergenic
AGATGGTCAGTGTTTAATGG+TGG 0.634003 1:-70710849 MsG0180003944.01.T01:CDS
AAAGACGACACACTCCTATG+TGG 0.634729 1:+70711631 None:intergenic
AGAAAAGGGTAGTTATGGCG+AGG 0.637570 1:-70711081 MsG0180003944.01.T01:CDS
GAGCGAGCTTACGAGCGAGA+GGG 0.639390 1:+70712006 None:intergenic
TTTCGTTTGGAATTGAGTCG+CGG 0.655974 1:+70710609 None:intergenic
ACAACAAAGCAGTATTATGG+TGG 0.664019 1:-70710695 MsG0180003944.01.T01:CDS
GATTCCATAATGGAAGCACT+CGG 0.666731 1:-70712098 MsG0180003944.01.T01:CDS
TGGAAAATCGACATTCGATG+GGG 0.680551 1:-70710873 MsG0180003944.01.T01:CDS
TGGAATCACTTGCTGCAAAG+GGG 0.683514 1:-70711420 MsG0180003944.01.T01:CDS
AGTACTCGTTTGGATTCACA+AGG 0.685833 1:-70710644 MsG0180003944.01.T01:three_prime_UTR
ACTCGTTTGGATTCACAAGG+CGG 0.687426 1:-70710641 MsG0180003944.01.T01:three_prime_UTR
TTTAACCGCGATGAAAGTGG+GGG 0.715077 1:+70710752 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTGATTATATCTCTAT+TGG - Chr1:70710715-70710734 MsG0180003944.01.T01:CDS 15.0%
!! AAAGATAAAGATCAAGTTTA+TGG - Chr1:70711091-70711110 MsG0180003944.01.T01:CDS 20.0%
!! ATATGATCCTAATAAGAAAA+GGG - Chr1:70711564-70711583 MsG0180003944.01.T01:CDS 20.0%
!! TATATGATCCTAATAAGAAA+AGG - Chr1:70711563-70711582 MsG0180003944.01.T01:CDS 20.0%
!!! AAAGTTTAATTGTTCAAGTT+GGG - Chr1:70711611-70711630 MsG0180003944.01.T01:CDS 20.0%
!!! ATAGTTGATTTAGTTTTGTA+GGG - Chr1:70710944-70710963 MsG0180003944.01.T01:CDS 20.0%
! AAAGATCAAGTTTATGGTAA+TGG - Chr1:70711097-70711116 MsG0180003944.01.T01:CDS 25.0%
! ACTTGAACAATTAAACTTTC+AGG + Chr1:70711610-70711629 None:intergenic 25.0%
! ATAATCAATAAACTACCTTG+AGG + Chr1:70710707-70710726 None:intergenic 25.0%
! CTACAAAACTAAATCAACTA+TGG + Chr1:70710945-70710964 None:intergenic 25.0%
! GAAAGTTTAATTGTTCAAGT+TGG - Chr1:70711610-70711629 MsG0180003944.01.T01:CDS 25.0%
! GTAAGACAAATAAGAAAAGA+GGG - Chr1:70711320-70711339 MsG0180003944.01.T01:CDS 25.0%
! TAAGACAAATAAGAAAAGAG+GGG - Chr1:70711321-70711340 MsG0180003944.01.T01:CDS 25.0%
! TAATAAGAAAAGGGTAGTTA+TGG - Chr1:70711573-70711592 MsG0180003944.01.T01:CDS 25.0%
! TGCTTAACAAAAATTTCGTT+TGG + Chr1:70712066-70712085 None:intergenic 25.0%
! TGGTATATTTACTGTTCTAA+CGG - Chr1:70711438-70711457 MsG0180003944.01.T01:CDS 25.0%
! TTGTCATTGATATAAAGCTA+GGG - Chr1:70710901-70710920 MsG0180003944.01.T01:CDS 25.0%
!! ATAACTACCCTTTTCTTATT+AGG + Chr1:70711574-70711593 None:intergenic 25.0%
!! CACAAATTTTCAATCATTGA+AGG + Chr1:70711697-70711716 None:intergenic 25.0%
!! TCCAAACAAATGCTAATTTT+GGG - Chr1:70712138-70712157 MsG0180003944.01.T01:CDS 25.0%
!! TTCCAAACAAATGCTAATTT+TGG - Chr1:70712137-70712156 MsG0180003944.01.T01:CDS 25.0%
!!! AAAGCTAGGGTTTATTTTTA+GGG - Chr1:70710914-70710933 MsG0180003944.01.T01:CDS 25.0%
!!! AAGTTTAATTGTTCAAGTTG+GGG - Chr1:70711612-70711631 MsG0180003944.01.T01:CDS 25.0%
!!! CATAGTTGATTTAGTTTTGT+AGG - Chr1:70710943-70710962 MsG0180003944.01.T01:CDS 25.0%
!!! TAAAGCTAGGGTTTATTTTT+AGG - Chr1:70710913-70710932 MsG0180003944.01.T01:CDS 25.0%
AAGGTCTATAAACAGAATGT+AGG + Chr1:70711678-70711697 None:intergenic 30.0%
ACCCAAAATTAGCATTTGTT+TGG + Chr1:70712142-70712161 None:intergenic 30.0%
AGAATTTGTGATAAGGCTAT+TGG - Chr1:70711190-70711209 MsG0180003944.01.T01:CDS 30.0%
AGGTCTATAAACAGAATGTA+GGG + Chr1:70711677-70711696 None:intergenic 30.0%
CAAAGTAGAAGATTCCATAA+TGG - Chr1:70710551-70710570 MsG0180003944.01.T01:three_prime_UTR 30.0%
CATTATGGAATCTTCTACTT+TGG + Chr1:70710553-70710572 None:intergenic 30.0%
CTGCTTCAAGAATTTGATAA+GGG - Chr1:70711298-70711317 MsG0180003944.01.T01:CDS 30.0%
GAAACAACAAAGCAGTATTA+TGG - Chr1:70711961-70711980 MsG0180003944.01.T01:intron 30.0%
GACTAAAACATTCTCTATCT+CGG - Chr1:70711753-70711772 MsG0180003944.01.T01:intron 30.0%
GGTAAGACAAATAAGAAAAG+AGG - Chr1:70711319-70711338 MsG0180003944.01.T01:CDS 30.0%
GTTGTCATTGATATAAAGCT+AGG - Chr1:70710900-70710919 MsG0180003944.01.T01:CDS 30.0%
TCTGCTTCAAGAATTTGATA+AGG - Chr1:70711297-70711316 MsG0180003944.01.T01:CDS 30.0%
TGAACAATTAAACTTTCAGG+AGG + Chr1:70711607-70711626 None:intergenic 30.0%
TTATCATTCTCGTTTAGATG+CGG - Chr1:70711273-70711292 MsG0180003944.01.T01:CDS 30.0%
TTTCGTTGAAAAATCCACAT+AGG - Chr1:70711014-70711033 MsG0180003944.01.T01:CDS 30.0%
!! ATTTTCTAGGAGCATAACTT+AGG - Chr1:70710983-70711002 MsG0180003944.01.T01:CDS 30.0%
!! TTTTCTTTTGCCGTCTTATT+CGG - Chr1:70711468-70711487 MsG0180003944.01.T01:CDS 30.0%
AAGAATGTGAAGCCATTAGA+AGG - Chr1:70711346-70711365 MsG0180003944.01.T01:CDS 35.0%
ACAACAAAGCAGTATTATGG+TGG - Chr1:70711964-70711983 MsG0180003944.01.T01:intron 35.0%
AGAATGTGAAGCCATTAGAA+GGG - Chr1:70711347-70711366 MsG0180003944.01.T01:CDS 35.0%
ATTTGTGATAAGGCTATTGG+TGG - Chr1:70711193-70711212 MsG0180003944.01.T01:CDS 35.0%
GACAAGTACAATGCAAGATT+CGG - Chr1:70711492-70711511 MsG0180003944.01.T01:CDS 35.0%
TAAGACGGCAAAAGAAAACA+TGG + Chr1:70711466-70711485 None:intergenic 35.0%
TAGATGACTAAGTACTCGTT+TGG - Chr1:70712005-70712024 MsG0180003944.01.T01:CDS 35.0%
TGAATTGGAGCAGAGTTTAT+TGG - Chr1:70711219-70711238 MsG0180003944.01.T01:CDS 35.0%
TTGGAAAATCGACATTCGAT+GGG - Chr1:70711785-70711804 MsG0180003944.01.T01:intron 35.0%
! ACTAGCTTCTTGTCAATTGT+GGG - Chr1:70712092-70712111 MsG0180003944.01.T01:CDS 35.0%
! AGAGTTAGGTTTTTGCATGA+TGG - Chr1:70711147-70711166 MsG0180003944.01.T01:CDS 35.0%
! GCTTGCTAGAATTTGTGATA+AGG - Chr1:70711183-70711202 MsG0180003944.01.T01:CDS 35.0%
! TACTAGCTTCTTGTCAATTG+TGG - Chr1:70712091-70712110 MsG0180003944.01.T01:CDS 35.0%
!! AATTGTTCAAGTTGGGGAAT+CGG - Chr1:70711618-70711637 MsG0180003944.01.T01:CDS 35.0%
!! CTTACTGTGGTTGATTTTCT+AGG - Chr1:70710970-70710989 MsG0180003944.01.T01:CDS 35.0%
!! GGTAATGGATTTGAGAATCT+AGG - Chr1:70711112-70711131 MsG0180003944.01.T01:CDS 35.0%
!!! GGAACTGTTTTTCAAGAGTT+AGG - Chr1:70711133-70711152 MsG0180003944.01.T01:CDS 35.0%
AAGCAGTATTATGGTGGTAG+TGG - Chr1:70711970-70711989 MsG0180003944.01.T01:intron 40.0%
ACACATTCGATCAGAAGAGT+CGG + Chr1:70710752-70710771 None:intergenic 40.0%
AGATGGTCAGTGTTTAATGG+TGG - Chr1:70711810-70711829 MsG0180003944.01.T01:intron 40.0%
AGTACTCGTTTGGATTCACA+AGG - Chr1:70712015-70712034 MsG0180003944.01.T01:CDS 40.0%
ATGCAGTTCATTCGGATTTG+TGG - Chr1:70711395-70711414 MsG0180003944.01.T01:CDS 40.0%
ATTTGATACGTTGGGGATTC+CGG + Chr1:70710616-70710635 None:intergenic 40.0%
CAGCAGTGGCATTATGATTA+TGG - Chr1:70711418-70711437 MsG0180003944.01.T01:CDS 40.0%
CGGCTGATATAATCTCGAAA+GGG - Chr1:70711638-70711657 MsG0180003944.01.T01:CDS 40.0%
CTTCTTGTCAATTGTGGGTT+AGG - Chr1:70712097-70712116 MsG0180003944.01.T01:CDS 40.0%
CTTGGAAAATCGACATTCGA+TGG - Chr1:70711784-70711803 MsG0180003944.01.T01:intron 40.0%
GGCTATTGGTGGAAATGAAT+TGG - Chr1:70711204-70711223 MsG0180003944.01.T01:CDS 40.0%
GGTAGATGGTCAGTGTTTAA+TGG - Chr1:70711807-70711826 MsG0180003944.01.T01:intron 40.0%
GTACTTGTCTCCGAATAAGA+CGG + Chr1:70711481-70711500 None:intergenic 40.0%
TAATCTCGAAAGGGAAGCTA+CGG - Chr1:70711647-70711666 MsG0180003944.01.T01:CDS 40.0%
TCGGCTGATATAATCTCGAA+AGG - Chr1:70711637-70711656 MsG0180003944.01.T01:CDS 40.0%
TCTATCTCGGATTATCCTCT+TGG - Chr1:70711766-70711785 MsG0180003944.01.T01:intron 40.0%
TGCTTGTGATGCAGTTCATT+CGG - Chr1:70711387-70711406 MsG0180003944.01.T01:CDS 40.0%
TGGAAAATCGACATTCGATG+GGG - Chr1:70711786-70711805 MsG0180003944.01.T01:intron 40.0%
TGTCTAGTGTGATGTTTGTG+TGG - Chr1:70710848-70710867 MsG0180003944.01.T01:CDS 40.0%
TTCAAACACGACCCTTCTAA+TGG + Chr1:70711361-70711380 None:intergenic 40.0%
TTTCATCGCGGTTAAAGGAT+GGG - Chr1:70711914-70711933 MsG0180003944.01.T01:intron 40.0%
! ATTGGAATCACTTGCTGCAA+AGG - Chr1:70711237-70711256 MsG0180003944.01.T01:CDS 40.0%
! GTTAGGTTTTTGCATGATGG+AGG - Chr1:70711150-70711169 MsG0180003944.01.T01:CDS 40.0%
! TTCTAGGAGCATAACTTAGG+TGG - Chr1:70710986-70711005 MsG0180003944.01.T01:CDS 40.0%
! TTGGAATCACTTGCTGCAAA+GGG - Chr1:70711238-70711257 MsG0180003944.01.T01:CDS 40.0%
! TTTCGTTTGGAATTGAGTCG+CGG + Chr1:70712053-70712072 None:intergenic 40.0%
! TTTTGTAGGGATGCTTACTG+TGG - Chr1:70710957-70710976 MsG0180003944.01.T01:CDS 40.0%
!! GATTCCATAATGGAAGCACT+CGG - Chr1:70710561-70710580 MsG0180003944.01.T01:three_prime_UTR 40.0%
!!! GAGAATGTTTTAGTCCATGC+AGG + Chr1:70711748-70711767 None:intergenic 40.0%
!!! TCGAATGTCGATTTTCCAAG+AGG + Chr1:70711784-70711803 None:intergenic 40.0%
AAAGACGACACACTCCTATG+TGG + Chr1:70711031-70711050 None:intergenic 45.0%
AACTCGTAATCGCATCCTCA+AGG - Chr1:70710689-70710708 MsG0180003944.01.T01:CDS 45.0%
ACTCGTTTGGATTCACAAGG+CGG - Chr1:70712018-70712037 MsG0180003944.01.T01:CDS 45.0%
AGAAAAGGGTAGTTATGGCG+AGG - Chr1:70711578-70711597 MsG0180003944.01.T01:CDS 45.0%
AGCGGAGATTTGATACGTTG+GGG + Chr1:70710623-70710642 None:intergenic 45.0%
CCTTTAACCGCGATGAAAGT+GGG + Chr1:70711912-70711931 None:intergenic 45.0%
CTTTAACCGCGATGAAAGTG+GGG + Chr1:70711911-70711930 None:intergenic 45.0%
CTTTCATCGCGGTTAAAGGA+TGG - Chr1:70711913-70711932 MsG0180003944.01.T01:intron 45.0%
GAAAAGGGTAGTTATGGCGA+GGG - Chr1:70711579-70711598 MsG0180003944.01.T01:CDS 45.0%
GAGCGGAGATTTGATACGTT+GGG + Chr1:70710624-70710643 None:intergenic 45.0%
TCCTTTAACCGCGATGAAAG+TGG + Chr1:70711913-70711932 None:intergenic 45.0%
TGAAGAACAACACTCTCGAC+AGG - Chr1:70711846-70711865 MsG0180003944.01.T01:intron 45.0%
TGACACGACACTAACACATG+TGG - Chr1:70710779-70710798 MsG0180003944.01.T01:CDS 45.0%
TGAGCGGAGATTTGATACGT+TGG + Chr1:70710625-70710644 None:intergenic 45.0%
TGAGGATGCGATTACGAGTT+TGG + Chr1:70710689-70710708 None:intergenic 45.0%
TGCAAATTCGTAAGCCCAGT+TGG + Chr1:70711541-70711560 None:intergenic 45.0%
TTGAATGTCCATCGCCAACT+GGG - Chr1:70711524-70711543 MsG0180003944.01.T01:CDS 45.0%
TTTAACCGCGATGAAAGTGG+GGG + Chr1:70711910-70711929 None:intergenic 45.0%
! GGGAGTACATTGACTGATAC+AGG + Chr1:70712169-70712188 None:intergenic 45.0%
! TGGAATCACTTGCTGCAAAG+GGG - Chr1:70711239-70711258 MsG0180003944.01.T01:CDS 45.0%
! TTCGTTTGGAATTGAGTCGC+GGG + Chr1:70712052-70712071 None:intergenic 45.0%
! TTGTATTTCTGCAGCCTGCA+TGG - Chr1:70711731-70711750 MsG0180003944.01.T01:intron 45.0%
!! GGTTTTTGCATGATGGAGGT+TGG - Chr1:70711154-70711173 MsG0180003944.01.T01:CDS 45.0%
ATTCGGATTTGTGGCAGCAG+TGG - Chr1:70711404-70711423 MsG0180003944.01.T01:CDS 50.0%
CCCACTTTCATCGCGGTTAA+AGG - Chr1:70711909-70711928 MsG0180003944.01.T01:intron 50.0%
GGATCCGAGTGCTTCCATTA+TGG + Chr1:70710568-70710587 None:intergenic 50.0%
GTTGAATGTCCATCGCCAAC+TGG - Chr1:70711523-70711542 MsG0180003944.01.T01:CDS 50.0%
TAGTTCCCCCACTTTCATCG+CGG - Chr1:70711902-70711921 MsG0180003944.01.T01:intron 50.0%
TCGACATTCGATGGGGTAGA+TGG - Chr1:70711793-70711812 MsG0180003944.01.T01:intron 50.0%
! GGAGTGTGTCGTCTTTTGCA+AGG - Chr1:70711035-70711054 MsG0180003944.01.T01:CDS 50.0%
!! ATGGAAGCACTCGGATCCAA+CGG - Chr1:70710570-70710589 MsG0180003944.01.T01:three_prime_UTR 50.0%
TACGTTGGGGATTCCGGTGA+CGG + Chr1:70710610-70710629 None:intergenic 55.0%
TTCGTAAGCCCAGTTGGCGA+TGG + Chr1:70711535-70711554 None:intergenic 55.0%
! CGAGAGGGAGAAGGTATGAG+CGG + Chr1:70710641-70710660 None:intergenic 55.0%
AGAGCGAGCTTACGAGCGAG+AGG + Chr1:70710657-70710676 None:intergenic 60.0%
GAGCGAGCTTACGAGCGAGA+GGG + Chr1:70710656-70710675 None:intergenic 60.0%
GCTTACGAGCGAGAGGGAGA+AGG + Chr1:70710650-70710669 None:intergenic 60.0%
GCACTCGGATCCAACGGTCC+AGG - Chr1:70710576-70710595 MsG0180003944.01.T01:three_prime_UTR 65.0%
! GGATTCCGGTGACGGCGAGG+AGG + Chr1:70710602-70710621 None:intergenic 70.0%
! TGGGGATTCCGGTGACGGCG+AGG + Chr1:70710605-70710624 None:intergenic 70.0%
CCAGGCCTCCTCGCCGTCAC+CGG - Chr1:70710594-70710613 MsG0180003944.01.T01:three_prime_UTR 75.0%
GGCGAGGAGGCCTGGACCGT+TGG + Chr1:70710589-70710608 None:intergenic 75.0%
!! CCGGTGACGGCGAGGAGGCC+TGG + Chr1:70710597-70710616 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 70710483 70712198 70710483 ID=MsG0180003944.01;Name=MsG0180003944.01
Chr1 mRNA 70710483 70712198 70710483 ID=MsG0180003944.01.T01;Parent=MsG0180003944.01;Name=MsG0180003944.01.T01;_AED=0.38;_eAED=0.38;_QI=0|1|0.5|1|1|1|2|187|415
Chr1 exon 70710483 70711689 70710483 ID=MsG0180003944.01.T01:exon:4647;Parent=MsG0180003944.01.T01
Chr1 exon 70711971 70712198 70711971 ID=MsG0180003944.01.T01:exon:4646;Parent=MsG0180003944.01.T01
Chr1 CDS 70711971 70712198 70711971 ID=MsG0180003944.01.T01:cds;Parent=MsG0180003944.01.T01
Chr1 CDS 70710670 70711689 70710670 ID=MsG0180003944.01.T01:cds;Parent=MsG0180003944.01.T01
Chr1 three_prime_UTR 70710483 70710669 70710483 ID=MsG0180003944.01.T01:three_prime_utr;Parent=MsG0180003944.01.T01
Gene Sequence

>MsG0180003944.01.T01

ATGGAAGAATGTGAGGTAATAGATTTATACGAACTTCACTATTCAGAATTATCATCACTATCTTCATCCAAAGTAGAAGATTCCATAATGGAAGCACTCGGATCCAACGGTCCAGGCCTCCTCGCCGTCACCGGAATCCCCAACGTATCAAATCTCCGCTCATACCTTCTCCCTCTCGCTCGTAAGCTCGCTCTTCTCGATCGCCAAACTCGTAATCGCATCCTCAAGGAGCATAACTTAGGTGGTGATGTTTCGTTGAAAAATCCACATAGGAGTGTGTCGTCTTTTGCAAGGCAATTGAATTATGCAAAAACACATTCTCAAGAGAAAGATAAAGATCAAGTTTATGGTAATGGATTTGAGAATCTAGGAACTGTTTTTCAAGAGTTAGGTTTTTGCATGATGGAGGTTGGACTTTTGCTTGCTAGAATTTGTGATAAGGCTATTGGTGGAAATGAATTGGAGCAGAGTTTATTGGAATCACTTGCTGCAAAGGGGCGTCTTATTCATTATCATTCTCGTTTAGATGCGGTTCTGCTTCAAGAATTTGATAAGGGTAAGACAAATAAGAAAAGAGGGGTTAAGAATGTGAAGCCATTAGAAGGGTCGTGTTTGAATTCTATTGCTTGTGATGCAGTTCATTCGGATTTGTGGCAGCAGTGGCATTATGATTATGGTATATTTACTGTTCTAACGGCTCCATGTTTTCTTTTGCCGTCTTATTCGGAGACAAGTACAATGCAAGATTCGGATAATTGTGTTGAATGTCCATCGCCAACTGGGCTTACGAATTTGCAGATATATGATCCTAATAAGAAAAGGGTAGTTATGGCGAGGGCTCCTCCTGAAAGTTTAATTGTTCAAGTTGGGGAATCGGCTGATATAATCTCGAAAGGGAAGCTACGGTCGACCCTACATTCTGTTTATAGACCTTCAATGATTGAAAATTTGTGCAGAGAAACTTTTGTTGTATTTCTGCAGCCTGCATGGACTAAAACATTCTCTATCTCGGATTATCCTCTTGGAAAATCGACATTCGATGGGGTAGATGGTCAGTGTTTAATGGTGGACGAGTTTGATGATGAAGAACAACACTCTCGACAGGATAATAACAAACTGAGTCTAGAAATTCAGAAAATAGTTCCCCCACTTTCATCGCGGTTAAAGGATGGGATGACTTTTGCTGAGTTCTCACGCGAAACAACAAAGCAGTATTATGGTGGTAGTGGTTTGCAATCAAATAGATGA
Protein sequence

>MsG0180003944.01.T01

MEECEVIDLYELHYSELSSLSSSKVEDSIMEALGSNGPGLLAVTGIPNVSNLRSYLLPLARKLALLDRQTRNRILKEHNLGGDVSLKNPHRSVSSFARQLNYAKTHSQEKDKDQVYGNGFENLGTVFQELGFCMMEVGLLLARICDKAIGGNELEQSLLESLAAKGRLIHYHSRLDAVLLQEFDKGKTNKKRGVKNVKPLEGSCLNSIACDAVHSDLWQQWHYDYGIFTVLTAPCFLLPSYSETSTMQDSDNCVECPSPTGLTNLQIYDPNKKRVVMARAPPESLIVQVGESADIISKGKLRSTLHSVYRPSMIENLCRETFVVFLQPAWTKTFSISDYPLGKSTFDGVDGQCLMVDEFDDEEQHSRQDNNKLSLEIQKIVPPLSSRLKDGMTFAEFSRETTKQYYGGSGLQSNR*