AlfalfaGEDB | MsG0180004314.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004314.01

KEGG: -
GO: GO:0005515
PFAM: PF00646 PF14299

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004314.01.T01	XP_024633601.2	67.712	319	85	4	1	303	1	317	1.13E-136	399

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004314.01.T01	Q9ZVQ6	36.482	307	153	10	1	302	1	270	2.61E-50	170

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004314.01.T01	A0A072UT85	67.712	319	85	4	1	303	1	317	5.39e-137	399

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180000005.01	MsG0180004314.01	PPI
MsG0180004314.01	MsG0180004440.01	PPI
MsG0180004314.01	MsG0680034993.01	PPI
MsG0180004314.01	MsG0180004418.01	PPI
MsG0180004314.01	MsG0680035152.01	PPI
MsG0180004314.01	MsG0380013038.01	PPI
MsG0180004314.01	MsG0180005448.01	PPI
MsG0180004314.01	MsG0180004419.01	PPI
MsG0180004314.01	MsG0380013028.01	PPI
MsG0180004314.01	MsG0180004420.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004314.01.T01	MTR_3g006850	67.712	319	85	4	1	303	1	317	1.37e-140	399
MsG0180004314.01.T01	MTR_1g100597	64.846	293	79	7	15	303	2	274	1.64e-120	347
MsG0180004314.01.T01	MTR_1g079230	62.284	289	90	3	17	303	4	275	3.90e-117	338
MsG0180004314.01.T01	MTR_1g079460	61.856	291	79	4	17	302	4	267	2.59e-115	333
MsG0180004314.01.T01	MTR_3g452600	61.538	286	81	5	17	301	10	267	1.72e-113	329
MsG0180004314.01.T01	MTR_1g079260	59.184	294	94	5	15	303	2	274	6.18e-111	323
MsG0180004314.01.T01	MTR_1g079180	61.168	291	78	6	17	301	4	265	6.94e-111	322
MsG0180004314.01.T01	MTR_1g079370	60.279	287	87	6	17	301	4	265	2.49e-110	321
MsG0180004314.01.T01	MTR_1g079850	57.718	298	101	6	15	303	2	283	1.23e-103	304
MsG0180004314.01.T01	MTR_1g100613	63.200	250	77	3	33	280	5	241	7.16e-102	298

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004314.01.T01	AT2G02360	36.482	307	153	10	1	302	1	270	2.66e-51	170
MsG0180004314.01.T01	AT1G80110	38.516	283	143	7	18	299	2	254	3.35e-49	164
MsG0180004314.01.T01	AT1G09155	38.225	293	155	10	20	301	3	280	1.25e-46	159
MsG0180004314.01.T01	AT2G02340	35.275	309	164	11	1	299	18	300	7.72e-46	157
MsG0180004314.01.T01	AT2G02230	34.277	318	162	11	14	299	29	331	1.34e-45	157
MsG0180004314.01.T01	AT2G02250	35.069	288	156	8	17	302	47	305	6.49e-44	152
MsG0180004314.01.T01	AT2G02240	35.374	294	156	8	14	304	58	320	1.65e-43	151
MsG0180004314.01.T01	AT1G56240	36.301	292	158	9	20	299	4	279	3.53e-42	147
MsG0180004314.01.T01	AT2G02350	31.922	307	174	9	1	302	8	284	6.16e-41	144
MsG0180004314.01.T01	AT1G56250	35.714	294	161	9	20	301	4	281	5.48e-40	141

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Find 72 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTATGGATTTATCCGTT+GGG	0.455755	1:-76354179	MsG0180004314.01.T01:CDS
AAGCCGCATACTGAGTATTT+GGG	0.368940	1:+76354244	None:intergenic
AAGCTGCCGCCATTCTTTCG+CGG	0.497928	1:+76356517	None:intergenic
AAGGAAAATCTTAGAAACAA+CGG	0.615790	1:+76356422	None:intergenic
AAGGGAAATTTCTATCTTGA+AGG	0.422985	1:-76353898	MsG0180004314.01.T01:CDS
AATCCTAATACTTTGTTACG+CGG	0.627348	1:+76354045	None:intergenic
AATGGCAAACCGCGAAAGAA+TGG	0.500944	1:-76356526	MsG0180004314.01.T01:CDS
ACCCCAACGGATAAATCCAT+AGG	0.649996	1:+76354177	None:intergenic
ACCTATGGATTTATCCGTTG+GGG	0.557323	1:-76354178	MsG0180004314.01.T01:CDS
ACTGTGAGATGATAGAGTCA+AGG	0.630484	1:+76356358	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGGAAATTTCTATCTTGA+AGG	-	Chr1:76356512-76356531	MsG0180004314.01.T01:CDS	30.0%
AATCCTAATACTTTGTTACG+CGG	+	Chr1:76356368-76356387	None:intergenic	30.0%
ACAGTTATGTAAACAATCTG+TGG	-	Chr1:76354663-76354682	MsG0180004314.01.T01:intron	30.0%
ACCTCTATATATTGTAGGTT+GGG	-	Chr1:76355201-76355220	MsG0180004314.01.T01:intron	30.0%
ATAGCATGTAACCAATACTT+TGG	+	Chr1:76354887-76354906	None:intergenic	30.0%
ATCTTGAAGGAATAGAAGTT+AGG	-	Chr1:76356525-76356544	MsG0180004314.01.T01:CDS	30.0%
ATGTAGTTGGAAATAGCAAA+AGG	-	Chr1:76355894-76355913	MsG0180004314.01.T01:intron	30.0%
CCACGAATTTCTCAAAAATT+AGG	-	Chr1:76354174-76354193	MsG0180004314.01.T01:CDS	30.0%
CTTGTACCTCTATATATTGT+AGG	-	Chr1:76355196-76355215	MsG0180004314.01.T01:intron	30.0%
GAATGGTACAAAGTTGTATT+GGG	+	Chr1:76355083-76355102	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	76353867	76356565	76353867	ID=MsG0180004314.01;Name=MsG0180004314.01
Chr1	mRNA	76353867	76356565	76353867	ID=MsG0180004314.01.T01;Parent=MsG0180004314.01;Name=MsG0180004314.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|1\|1\|1\|3\|0\|304
Chr1	exon	76356272	76356565	76356272	ID=MsG0180004314.01.T01:exon:12631;Parent=MsG0180004314.01.T01
Chr1	exon	76355910	76356055	76355910	ID=MsG0180004314.01.T01:exon:12630;Parent=MsG0180004314.01.T01
Chr1	exon	76353867	76354341	76353867	ID=MsG0180004314.01.T01:exon:12629;Parent=MsG0180004314.01.T01
Chr1	CDS	76356272	76356565	76356272	ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01
Chr1	CDS	76355910	76356055	76355910	ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01
Chr1	CDS	76353867	76354341	76353867	ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01

Gene Sequence

>MsG0180004314.01.T01

ATGGCAAAAACCAGAAGAATGGCAAACCGCGAAAGAATGGCGGCAGCTTTTCATGAATTGACGGAAGGATGCCTCTCTGCCATTCTGTCTCATACCACTCCCGTTGACGTTTGCAGGCTATCCGTTGTTTCTAAGATTTTCCTTTCTGCTGCCAATTCCGACACCATTTGGAACCACTTCCGTTTCCTTGACTCTATCATCTCACAGTCTCCTTCCCTTGCCAACGCTCCCACCAAAAAGGCTCTCTATCTTGCTCTCTCTGATCGCCCTATCATCATCGATCATGGTCGAAAGAGCTTTCAATTGGATAGGAAGAGTGGAAAAATATGTTACATGCGTGCTGCTAAATCTCTCACCATTAATTGCAGTGATGGTGATAGTGATGAAGATGATGATGAGTTGCTTGTTAAGAAAGAGTGGATTCCTATGCATGATTCCAGGTTTCCTGAAGTTTCTAAGCTTTGTCTTGTGTACTCACTAGAAATTCGTGGGATGATTAACACTCTTTCCTTGTCCCCAAATACTCAGTATGCGGCTTATCTCGTGTTCAAGATGATTAATGCCTATGGATTTGAGAATGAACCTATGGATTTATCCGTTGGGGTTGAAGGTGGACATAGCAGCACTAAATCTGTCTATTTGCACAACCGAGACGATCGTGTCATGCAAGCAAGTTGTAAATGTGGTCGTCAGTTCCATCATCATGCGAGGAGGCCGCGTAACAAAGTATTAGGATTGCAACGTCCTAGTGTGAGAAGTGATGGGTGGTTGGAGATTGAGATGGGAGAGTTCTTCAATTCAAGTCTTGAAGATGAAGAAATCCAAATGAGTGTTGTCGAGAAATTTGATAGTGGCAATGAGAAGGGAAATTTCTATCTTGAAGGAATAGAAGTTAGGCTTAAGAAAGACAAGTAA

Protein sequence

>MsG0180004314.01.T01

MAKTRRMANRERMAAAFHELTEGCLSAILSHTTPVDVCRLSVVSKIFLSAANSDTIWNHFRFLDSIISQSPSLANAPTKKALYLALSDRPIIIDHGRKSFQLDRKSGKICYMRAAKSLTINCSDGDSDEDDDELLVKKEWIPMHDSRFPEVSKLCLVYSLEIRGMINTLSLSPNTQYAAYLVFKMINAYGFENEPMDLSVGVEGGHSSTKSVYLHNRDDRVMQASCKCGRQFHHHARRPRNKVLGLQRPSVRSDGWLEIEMGEFFNSSLEDEEIQMSVVEKFDSGNEKGNFYLEGIEVRLKKDK*