AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004314.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004314.01.T01 MTR_3g006850 67.712 319 85 4 1 303 1 317 1.37e-140 399
MsG0180004314.01.T01 MTR_1g100597 64.846 293 79 7 15 303 2 274 1.64e-120 347
MsG0180004314.01.T01 MTR_1g079230 62.284 289 90 3 17 303 4 275 3.90e-117 338
MsG0180004314.01.T01 MTR_1g079460 61.856 291 79 4 17 302 4 267 2.59e-115 333
MsG0180004314.01.T01 MTR_3g452600 61.538 286 81 5 17 301 10 267 1.72e-113 329
MsG0180004314.01.T01 MTR_1g079260 59.184 294 94 5 15 303 2 274 6.18e-111 323
MsG0180004314.01.T01 MTR_1g079180 61.168 291 78 6 17 301 4 265 6.94e-111 322
MsG0180004314.01.T01 MTR_1g079370 60.279 287 87 6 17 301 4 265 2.49e-110 321
MsG0180004314.01.T01 MTR_1g079850 57.718 298 101 6 15 303 2 283 1.23e-103 304
MsG0180004314.01.T01 MTR_1g100613 63.200 250 77 3 33 280 5 241 7.16e-102 298
MsG0180004314.01.T01 MTR_1g079200 58.219 292 84 7 17 301 4 264 2.92e-100 295
MsG0180004314.01.T01 MTR_2g079100 57.241 290 89 6 16 302 8 265 7.76e-99 291
MsG0180004314.01.T01 MTR_3g450990 54.967 302 91 7 17 301 10 283 2.02e-98 291
MsG0180004314.01.T01 MTR_1g079250 56.081 296 106 4 15 303 2 280 3.24e-98 291
MsG0180004314.01.T01 MTR_1g026510 54.054 296 117 6 15 303 2 285 1.08e-97 290
MsG0180004314.01.T01 MTR_1g079380 58.188 287 93 5 17 301 4 265 1.59e-97 289
MsG0180004314.01.T01 MTR_1g079350 50.333 300 110 8 15 301 2 275 3.90e-82 249
MsG0180004314.01.T01 MTR_1g079410 47.333 300 123 6 11 302 2 274 8.56e-80 243
MsG0180004314.01.T01 MTR_1g076870 86.131 137 18 1 148 283 2 138 1.64e-78 235
MsG0180004314.01.T01 MTR_7g096300 52.982 285 93 8 15 291 2 253 1.51e-75 246
MsG0180004314.01.T01 MTR_1g079340 44.201 319 139 8 1 301 28 325 2.59e-73 228
MsG0180004314.01.T01 MTR_1g079140 41.479 311 151 9 11 301 7 306 4.26e-66 209
MsG0180004314.01.T01 MTR_1g079400 41.196 301 140 7 11 301 6 279 2.68e-61 196
MsG0180004314.01.T01 MTR_1g078470 58.642 162 46 5 140 301 44 184 1.41e-50 166
MsG0180004314.01.T01 MTR_7g112550 40.878 296 139 13 20 301 10 283 2.50e-50 168
MsG0180004314.01.T01 MTR_1g083580 37.329 292 152 11 20 301 11 281 1.95e-49 166
MsG0180004314.01.T01 MTR_1g079350 55.921 152 47 4 15 157 2 142 1.11e-42 146
MsG0180004314.01.T01 MTR_1g079240 45.116 215 77 6 91 301 2 179 6.50e-42 143
MsG0180004314.01.T01 MTR_7g112560 32.872 289 152 9 20 301 4 257 2.78e-41 144
MsG0180004314.01.T01 MTR_1g079210 54.762 126 54 1 149 274 7 129 2.51e-36 127
MsG0180004314.01.T01 MTR_3g058640 57.724 123 34 3 143 264 1 106 2.87e-35 123
MsG0180004314.01.T01 MTR_7g096310 55.319 141 46 3 68 206 38 163 9.35e-35 124
MsG0180004314.01.T01 MTR_2g042540 64.423 104 33 2 15 114 2 105 2.10e-34 122
MsG0180004314.01.T01 MTR_4g023570 31.707 287 138 10 20 301 4 237 1.36e-32 120
MsG0180004314.01.T01 MTR_1g079130 37.073 205 98 6 112 301 1 189 7.36e-31 115
MsG0180004314.01.T01 MTR_1g078460 63.529 85 29 1 16 98 66 150 3.74e-28 106
MsG0180004314.01.T01 MTR_1g079220 56.627 83 19 3 185 267 1 66 1.14e-19 82.0
MsG0180004314.01.T01 MTR_7g103380 26.061 165 106 5 8 167 10 163 4.50e-15 74.7
MsG0180004314.01.T01 MTR_1g101390 26.168 214 130 6 19 215 30 232 8.82e-15 73.6
MsG0180004314.01.T01 MTR_1g079390 64.706 51 15 2 246 296 8 55 1.85e-13 64.3
MsG0180004314.01.T01 MTR_1g079190 35.714 126 31 2 18 110 27 135 6.64e-13 66.2
MsG0180004314.01.T01 MTR_1g079190 61.702 47 17 1 247 293 129 174 6.14e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004314.01.T01 AT2G02360 36.482 307 153 10 1 302 1 270 2.66e-51 170
MsG0180004314.01.T01 AT1G80110 38.516 283 143 7 18 299 2 254 3.35e-49 164
MsG0180004314.01.T01 AT1G09155 38.225 293 155 10 20 301 3 280 1.25e-46 159
MsG0180004314.01.T01 AT2G02340 35.275 309 164 11 1 299 18 300 7.72e-46 157
MsG0180004314.01.T01 AT2G02230 34.277 318 162 11 14 299 29 331 1.34e-45 157
MsG0180004314.01.T01 AT2G02250 35.069 288 156 8 17 302 47 305 6.49e-44 152
MsG0180004314.01.T01 AT2G02240 35.374 294 156 8 14 304 58 320 1.65e-43 151
MsG0180004314.01.T01 AT1G56240 36.301 292 158 9 20 299 4 279 3.53e-42 147
MsG0180004314.01.T01 AT2G02350 31.922 307 174 9 1 302 8 284 6.16e-41 144
MsG0180004314.01.T01 AT1G56250 35.714 294 161 9 20 301 4 281 5.48e-40 141
MsG0180004314.01.T01 AT2G02310 31.164 292 166 8 15 301 43 304 2.27e-33 124
MsG0180004314.01.T01 AT2G02320 32.069 290 160 8 19 302 42 300 2.66e-33 124
MsG0180004314.01.T01 AT2G02300 30.769 299 154 8 14 304 31 284 2.82e-32 121
MsG0180004314.01.T01 AT5G24560 33.673 196 106 6 17 205 3 181 5.44e-27 105
MsG0180004314.01.T01 AT3G53000 23.203 306 171 12 13 296 17 280 9.68e-18 82.0
MsG0180004314.01.T01 AT2G02350 31.325 166 92 5 140 302 21 167 1.30e-16 76.6
MsG0180004314.01.T01 AT1G12710 23.200 250 146 7 7 236 18 241 5.46e-13 68.6
MsG0180004314.01.T01 AT1G63090 22.490 249 147 7 8 236 17 239 5.10e-12 65.5
MsG0180004314.01.T01 AT3G61060 21.198 217 139 6 19 216 27 230 4.94e-11 62.8

Find 72 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCCGCATACTGAGTATT+TGG 0.215477 1:+76354243 None:intergenic
CCACTCCCGTTGACGTTTGC+AGG 0.247260 1:-76356449 MsG0180004314.01.T01:CDS
ATCACCATCACTGCAATTAA+TGG 0.269136 1:+76355972 None:intergenic
TTCGCGGTTTGCCATTCTTC+TGG 0.286298 1:+76356533 None:intergenic
TGCCAACGCTCCCACCAAAA+AGG 0.334601 1:-76356325 MsG0180004314.01.T01:CDS
CTGCCAATTCCGACACCATT+TGG 0.343874 1:-76356395 MsG0180004314.01.T01:CDS
AGGCCGCGTAACAAAGTATT+AGG 0.351590 1:-76354048 MsG0180004314.01.T01:CDS
ATTTCTCGACAACACTCATT+TGG 0.353535 1:+76353938 None:intergenic
CGACCATGATCGATGATGAT+AGG 0.364184 1:+76356276 None:intergenic
AAGCCGCATACTGAGTATTT+GGG 0.368940 1:+76354244 None:intergenic
TGAAATTCAGAGCTTTCAAT+TGG 0.378044 1:-76356043 MsG0180004314.01.T01:intron
GTTCCAAATGGTGTCGGAAT+TGG 0.384702 1:+76356392 None:intergenic
ATCTTGAAGGAATAGAAGTT+AGG 0.408541 1:-76353885 MsG0180004314.01.T01:CDS
GGATTCCTATGCATGATTCC+AGG 0.414501 1:-76355909 MsG0180004314.01.T01:intron
TTCAAGATGATTAATGCCTA+TGG 0.419380 1:-76354213 MsG0180004314.01.T01:CDS
AGACAAAGCTTAGAAACTTC+AGG 0.420389 1:+76354315 None:intergenic
AAGGGAAATTTCTATCTTGA+AGG 0.422985 1:-76353898 MsG0180004314.01.T01:CDS
GATTTATCCGTTGGGGTTGA+AGG 0.438990 1:-76354171 MsG0180004314.01.T01:CDS
AACCTATGGATTTATCCGTT+GGG 0.455755 1:-76354179 MsG0180004314.01.T01:CDS
GATAGCCTGCAAACGTCAAC+GGG 0.455839 1:+76356444 None:intergenic
GAACCTATGGATTTATCCGT+TGG 0.468055 1:-76354180 MsG0180004314.01.T01:CDS
CTTGCATGACACGATCGTCT+CGG 0.484226 1:+76354112 None:intergenic
AAGCTGCCGCCATTCTTTCG+CGG 0.497928 1:+76356517 None:intergenic
CGCCCTATCATCATCGATCA+TGG 0.500622 1:-76356279 MsG0180004314.01.T01:CDS
TGGATTTGAGAATGAACCTA+TGG 0.500804 1:-76354193 MsG0180004314.01.T01:CDS
AATGGCAAACCGCGAAAGAA+TGG 0.500944 1:-76356526 MsG0180004314.01.T01:CDS
AGTTGCTTGTTAAGAAAGAG+TGG 0.502113 1:-76355930 MsG0180004314.01.T01:CDS
CGTCCTAGTGTGAGAAGTGA+TGG 0.512151 1:-76354018 MsG0180004314.01.T01:CDS
GCGGCCTCCTCGCATGATGA+TGG 0.514601 1:+76354064 None:intergenic
CTCACCATTAATTGCAGTGA+TGG 0.514752 1:-76355976 MsG0180004314.01.T01:CDS
CCTGCAAACGTCAACGGGAG+TGG 0.523059 1:+76356449 None:intergenic
TTTGATAGTGGCAATGAGAA+GGG 0.525354 1:-76353916 MsG0180004314.01.T01:CDS
ATTTGATAGTGGCAATGAGA+AGG 0.527724 1:-76353917 MsG0180004314.01.T01:CDS
GTGGGAGCGTTGGCAAGGGA+AGG 0.528441 1:+76356333 None:intergenic
GGGTGGTTGGAGATTGAGAT+GGG 0.540728 1:-76353997 MsG0180004314.01.T01:CDS
GAAACGGAAGTGGTTCCAAA+TGG 0.541467 1:+76356380 None:intergenic
TGGGTGGTTGGAGATTGAGA+TGG 0.542699 1:-76353998 MsG0180004314.01.T01:CDS
GACCATGATCGATGATGATA+GGG 0.546493 1:+76356277 None:intergenic
AGATGATAGAGTCAAGGAAA+CGG 0.549210 1:+76356364 None:intergenic
TGTGAGAAGTGATGGGTGGT+TGG 0.556049 1:-76354010 MsG0180004314.01.T01:CDS
ACCTATGGATTTATCCGTTG+GGG 0.557323 1:-76354178 MsG0180004314.01.T01:CDS
GTGTACTCACTAGAAATTCG+TGG 0.562884 1:-76354291 MsG0180004314.01.T01:CDS
TAGAGTCAAGGAAACGGAAG+TGG 0.562894 1:+76356370 None:intergenic
CACCCATCACTTCTCACACT+AGG 0.570746 1:+76354015 None:intergenic
GTTGTCGAGAAATTTGATAG+TGG 0.574516 1:-76353928 MsG0180004314.01.T01:CDS
TTATCCGTTGGGGTTGAAGG+TGG 0.579797 1:-76354168 MsG0180004314.01.T01:CDS
GGGAGTGGTATGAGACAGAA+TGG 0.583998 1:+76356464 None:intergenic
AGGTTCATTCTCAAATCCAT+AGG 0.584278 1:+76354197 None:intergenic
TTCAGAGCTTTCAATTGGAT+AGG 0.591367 1:-76356038 MsG0180004314.01.T01:intron
TTTCAATTGGATAGGAAGAG+TGG 0.595118 1:-76356030 MsG0180004314.01.T01:CDS
TTTGGTGGGAGCGTTGGCAA+GGG 0.595797 1:+76356329 None:intergenic
CATCATCGATCATGGTCGAA+AGG 0.598086 1:-76356271 MsG0180004314.01.T01:intron
GGATAGCCTGCAAACGTCAA+CGG 0.602677 1:+76356443 None:intergenic
TGTCGGAATTGGCAGCAGAA+AGG 0.614407 1:+76356403 None:intergenic
GAAGTGGTTCCAAATGGTGT+CGG 0.614896 1:+76356386 None:intergenic
TGTACTCACTAGAAATTCGT+GGG 0.615662 1:-76354290 MsG0180004314.01.T01:CDS
AAGGAAAATCTTAGAAACAA+CGG 0.615790 1:+76356422 None:intergenic
GCTAACCTGGAATCATGCAT+AGG 0.615981 1:+76355904 None:intergenic
AATCCTAATACTTTGTTACG+CGG 0.627348 1:+76354045 None:intergenic
AGCCGCATACTGAGTATTTG+GGG 0.630234 1:+76354245 None:intergenic
ACTGTGAGATGATAGAGTCA+AGG 0.630484 1:+76356358 None:intergenic
GTCCCCAAATACTCAGTATG+CGG 0.634233 1:-76354247 MsG0180004314.01.T01:CDS
ATGTCCACCTTCAACCCCAA+CGG 0.637315 1:+76354164 None:intergenic
ATACTGAGTATTTGGGGACA+AGG 0.640405 1:+76354251 None:intergenic
GTCAGTTCCATCATCATGCG+AGG 0.645971 1:-76354071 MsG0180004314.01.T01:CDS
ACCCCAACGGATAAATCCAT+AGG 0.649996 1:+76354177 None:intergenic
GGCAAACCGCGAAAGAATGG+CGG 0.660050 1:-76356523 MsG0180004314.01.T01:CDS
GTCCTAGTGTGAGAAGTGAT+GGG 0.691802 1:-76354017 MsG0180004314.01.T01:CDS
ATGCAAGCAAGTTGTAAATG+TGG 0.695894 1:-76354096 MsG0180004314.01.T01:CDS
AGTTCCATCATCATGCGAGG+AGG 0.702717 1:-76354068 MsG0180004314.01.T01:CDS
TATGAGACAGAATGGCAGAG+AGG 0.719068 1:+76356472 None:intergenic
CTAGTGTGAGAAGTGATGGG+TGG 0.729446 1:-76354014 MsG0180004314.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTCATAAATATATATT+AGG + Chr1:76355377-76355396 None:intergenic 10.0%
!! ATTTATGAAACATTTAAATA+TGG - Chr1:76355384-76355403 MsG0180004314.01.T01:intron 10.0%
!! CCTTATAATAAAAAAAAATT+CGG + Chr1:76354862-76354881 None:intergenic 10.0%
!! GAAAAAAATGTATAATAAAT+AGG - Chr1:76355847-76355866 MsG0180004314.01.T01:intron 10.0%
!! TGTATTTATAACTTAAATAA+AGG - Chr1:76355157-76355176 MsG0180004314.01.T01:intron 10.0%
!! TTTATGAAACATTTAAATAT+GGG - Chr1:76355385-76355404 MsG0180004314.01.T01:intron 10.0%
!! AAAGAAATAATTAATGTAGT+TGG - Chr1:76355881-76355900 MsG0180004314.01.T01:intron 15.0%
!! AACTATATATTAAAAATGAG+AGG - Chr1:76354758-76354777 MsG0180004314.01.T01:intron 15.0%
!! ACTATATATTAAAAATGAGA+GGG - Chr1:76354759-76354778 MsG0180004314.01.T01:intron 15.0%
!! CTATACAATTCATAAAATTT+AGG - Chr1:76355126-76355145 MsG0180004314.01.T01:intron 15.0%
!! TTATGAAACATTTAAATATG+GGG - Chr1:76355386-76355405 MsG0180004314.01.T01:intron 15.0%
!!! AATTAAACTCAACATATTTT+AGG - Chr1:76354693-76354712 MsG0180004314.01.T01:intron 15.0%
!!! ATTAAACTCAACATATTTTA+GGG - Chr1:76354694-76354713 MsG0180004314.01.T01:intron 15.0%
!!! CCGAATTTTTTTTTATTATA+AGG - Chr1:76354859-76354878 MsG0180004314.01.T01:intron 15.0%
!!! TAGATTTTTTAAGATGTTTT+CGG - Chr1:76354322-76354341 MsG0180004314.01.T01:CDS 15.0%
!!! TGAATGATTTATTCATTTTT+TGG - Chr1:76354719-76354738 MsG0180004314.01.T01:intron 15.0%
!! AACTATTGTAATAAAGCAAA+TGG - Chr1:76355565-76355584 MsG0180004314.01.T01:intron 20.0%
!! AATATGTTTGCAATTACTAT+TGG - Chr1:76355754-76355773 MsG0180004314.01.T01:intron 20.0%
!! GACAAAAAAAATTCTCAAAA+GGG - Chr1:76355931-76355950 MsG0180004314.01.T01:CDS 20.0%
!! TATGAAACATTTAAATATGG+GGG - Chr1:76355387-76355406 MsG0180004314.01.T01:intron 20.0%
!! TTTACATCTTAAAAAACCAA+TGG + Chr1:76354295-76354314 None:intergenic 20.0%
!!! ATGAAGATATATTTTTTGAG+AGG + Chr1:76354636-76354655 None:intergenic 20.0%
!!! ATTCTCGTTATTTTTTCAAA+AGG + Chr1:76355711-76355730 None:intergenic 20.0%
!!! GATTTATTCATTTTTTGGAA+TGG - Chr1:76354724-76354743 MsG0180004314.01.T01:intron 20.0%
!!! TACTAAAGTTTTGAAAATTC+AGG + Chr1:76355620-76355639 None:intergenic 20.0%
!!! TTCTCGTTATTTTTTCAAAA+GGG + Chr1:76355710-76355729 None:intergenic 20.0%
!!! TTTTATTAAATCGAGTACAA+AGG + Chr1:76355658-76355677 None:intergenic 20.0%
! AAGGAAAATCTTAGAAACAA+CGG + Chr1:76353991-76354010 None:intergenic 25.0%
! AATAACGAGAATTAAGATAG+TGG - Chr1:76355719-76355738 MsG0180004314.01.T01:intron 25.0%
! AATGTTAATGAAAACATGTC+GGG + Chr1:76354613-76354632 None:intergenic 25.0%
! ATTATAAGGTTCCAAAGTAT+TGG - Chr1:76354873-76354892 MsG0180004314.01.T01:intron 25.0%
! CACCAATGAATTAAATGATA+AGG + Chr1:76354263-76354282 None:intergenic 25.0%
! CACGAATTTCTCAAAAATTA+GGG - Chr1:76354175-76354194 MsG0180004314.01.T01:CDS 25.0%
! GAATGTTAATGAAAACATGT+CGG + Chr1:76354614-76354633 None:intergenic 25.0%
! GGACAAAAAAAATTCTCAAA+AGG - Chr1:76355930-76355949 MsG0180004314.01.T01:CDS 25.0%
! TATCATTTAATTCATTGGTG+TGG - Chr1:76354263-76354282 MsG0180004314.01.T01:CDS 25.0%
!! TCAAAAGGGTCTTATAATTA+GGG - Chr1:76355945-76355964 MsG0180004314.01.T01:CDS 25.0%
!! TCTCCATTTTCAAAAAGAAA+TGG - Chr1:76355460-76355479 MsG0180004314.01.T01:intron 25.0%
!!! AATTTTATCCTTTAAGAGAG+AGG - Chr1:76355783-76355802 MsG0180004314.01.T01:intron 25.0%
!!! ATTTCTTTTTGAAAATGGAG+AGG + Chr1:76355461-76355480 None:intergenic 25.0%
!!! ATTTTATCCTTTAAGAGAGA+GGG - Chr1:76355784-76355803 MsG0180004314.01.T01:intron 25.0%
!!! TCACCATTTCTTTTTGAAAA+TGG + Chr1:76355466-76355485 None:intergenic 25.0%
AAGGGAAATTTCTATCTTGA+AGG - Chr1:76356512-76356531 MsG0180004314.01.T01:CDS 30.0%
AATCCTAATACTTTGTTACG+CGG + Chr1:76356368-76356387 None:intergenic 30.0%
ACAGTTATGTAAACAATCTG+TGG - Chr1:76354663-76354682 MsG0180004314.01.T01:intron 30.0%
ACCTCTATATATTGTAGGTT+GGG - Chr1:76355201-76355220 MsG0180004314.01.T01:intron 30.0%
ATAGCATGTAACCAATACTT+TGG + Chr1:76354887-76354906 None:intergenic 30.0%
ATCTTGAAGGAATAGAAGTT+AGG - Chr1:76356525-76356544 MsG0180004314.01.T01:CDS 30.0%
ATGTAGTTGGAAATAGCAAA+AGG - Chr1:76355894-76355913 MsG0180004314.01.T01:intron 30.0%
CCACGAATTTCTCAAAAATT+AGG - Chr1:76354174-76354193 MsG0180004314.01.T01:CDS 30.0%
CTTGTACCTCTATATATTGT+AGG - Chr1:76355196-76355215 MsG0180004314.01.T01:intron 30.0%
GAATGGTACAAAGTTGTATT+GGG + Chr1:76355083-76355102 None:intergenic 30.0%
TACCTCTATATATTGTAGGT+TGG - Chr1:76355200-76355219 MsG0180004314.01.T01:intron 30.0%
TCAAAAGGGTCCTATAATTA+GGG + Chr1:76355696-76355715 None:intergenic 30.0%
TGAAATTCAGAGCTTTCAAT+TGG - Chr1:76354367-76354386 MsG0180004314.01.T01:intron 30.0%
TGAATGGTACAAAGTTGTAT+TGG + Chr1:76355084-76355103 None:intergenic 30.0%
TGCAGATATAATAACAATGC+AGG + Chr1:76355982-76356001 None:intergenic 30.0%
TGGAGAAAATAAAGAGTAGA+CGG - Chr1:76355585-76355604 MsG0180004314.01.T01:intron 30.0%
TGTAGTTGGAAATAGCAAAA+GGG - Chr1:76355895-76355914 MsG0180004314.01.T01:intron 30.0%
TTCAAAAGGGTCCTATAATT+AGG + Chr1:76355697-76355716 None:intergenic 30.0%
TTCAAGATGATTAATGCCTA+TGG - Chr1:76356197-76356216 MsG0180004314.01.T01:intron 30.0%
! AGCAAAAGGGTCTTATAAAA+AGG - Chr1:76355908-76355927 MsG0180004314.01.T01:intron 30.0%
! CTCAAAAGGGTCTTATAATT+AGG - Chr1:76355944-76355963 MsG0180004314.01.T01:CDS 30.0%
! CTCCTCTGACTTTAAATTTT+TGG + Chr1:76355240-76355259 None:intergenic 30.0%
! GCAAAAGGGTCTTATAAAAA+GGG - Chr1:76355909-76355928 MsG0180004314.01.T01:intron 30.0%
! GCTCTGAATTTCATAATTCA+AGG + Chr1:76354360-76354379 None:intergenic 30.0%
! GGCCTTATCATTTAATTCAT+TGG - Chr1:76354258-76354277 MsG0180004314.01.T01:CDS 30.0%
! TCTGTCTTTGCAATTTTTAC+AGG - Chr1:76356069-76356088 MsG0180004314.01.T01:intron 30.0%
!! ACATTCTTTTTGAGCGAAAA+AGG - Chr1:76355273-76355292 MsG0180004314.01.T01:intron 30.0%
!! CCTAATTTTTGAGAAATTCG+TGG + Chr1:76354177-76354196 None:intergenic 30.0%
AAATAAAGAGTAGACGGAAG+AGG - Chr1:76355591-76355610 MsG0180004314.01.T01:intron 35.0%
AACCTATGGATTTATCCGTT+GGG - Chr1:76356231-76356250 MsG0180004314.01.T01:intron 35.0%
AGAAAACTTAAGAGCTAACC+TGG + Chr1:76354522-76354541 None:intergenic 35.0%
AGACAAAGCTTAGAAACTTC+AGG + Chr1:76356098-76356117 None:intergenic 35.0%
AGATGATAGAGTCAAGGAAA+CGG + Chr1:76354049-76354068 None:intergenic 35.0%
AGGCCTAATATGAGAGAAAT+TGG + Chr1:76354243-76354262 None:intergenic 35.0%
AGGGAATAAAAGTAGTACCA+GGG - Chr1:76354778-76354797 MsG0180004314.01.T01:intron 35.0%
AGGTTCATTCTCAAATCCAT+AGG + Chr1:76356216-76356235 None:intergenic 35.0%
ATCACCATCACTGCAATTAA+TGG + Chr1:76354441-76354460 None:intergenic 35.0%
ATCTAAGAATACTGCACATG+TGG + Chr1:76354955-76354974 None:intergenic 35.0%
ATGCAAGCAAGTTGTAAATG+TGG - Chr1:76356314-76356333 MsG0180004314.01.T01:CDS 35.0%
ATTGGTGTGGAAAAAACCAT+TGG - Chr1:76354276-76354295 MsG0180004314.01.T01:CDS 35.0%
ATTTCTCGACAACACTCATT+TGG + Chr1:76356475-76356494 None:intergenic 35.0%
CATTCATTAATCCAAGCACT+AGG - Chr1:76355098-76355117 MsG0180004314.01.T01:intron 35.0%
CATTTAAATATGGGGGACTA+TGG - Chr1:76355394-76355413 MsG0180004314.01.T01:intron 35.0%
CTAGTGCTTGGATTAATGAA+TGG + Chr1:76355100-76355119 None:intergenic 35.0%
GTTGTCGAGAAATTTGATAG+TGG - Chr1:76356482-76356501 MsG0180004314.01.T01:CDS 35.0%
TCCCAACCTACAATATATAG+AGG + Chr1:76355205-76355224 None:intergenic 35.0%
TGGAATGACTTTCAAACGAT+GGG + Chr1:76354935-76354954 None:intergenic 35.0%
TGGATTTGAGAATGAACCTA+TGG - Chr1:76356217-76356236 MsG0180004314.01.T01:intron 35.0%
TGTACTCACTAGAAATTCGT+GGG - Chr1:76356120-76356139 MsG0180004314.01.T01:intron 35.0%
TTCAGAGCTTTCAATTGGAT+AGG - Chr1:76354372-76354391 MsG0180004314.01.T01:intron 35.0%
TTTCAATTGGATAGGAAGAG+TGG - Chr1:76354380-76354399 MsG0180004314.01.T01:intron 35.0%
! AAGGGTCTTATAATTAGGGA+CGG - Chr1:76355949-76355968 MsG0180004314.01.T01:CDS 35.0%
! ACAGTGCCTCAAATATTTTG+GGG - Chr1:76354835-76354854 MsG0180004314.01.T01:intron 35.0%
! AGTTGCTTGTTAAGAAAGAG+TGG - Chr1:76354480-76354499 MsG0180004314.01.T01:intron 35.0%
! ATATATTGTAGGTTGGGATC+AGG - Chr1:76355207-76355226 MsG0180004314.01.T01:intron 35.0%
! CACAGTGCCTCAAATATTTT+GGG - Chr1:76354834-76354853 MsG0180004314.01.T01:intron 35.0%
! GGCCAAAAATTTAAAGTCAG+AGG - Chr1:76355235-76355254 MsG0180004314.01.T01:intron 35.0%
! TATATTGTAGGTTGGGATCA+GGG - Chr1:76355208-76355227 MsG0180004314.01.T01:intron 35.0%
!! ATTTGATAGTGGCAATGAGA+AGG - Chr1:76356493-76356512 MsG0180004314.01.T01:CDS 35.0%
!! TTTGATAGTGGCAATGAGAA+GGG - Chr1:76356494-76356513 MsG0180004314.01.T01:CDS 35.0%
AAACCAACCCTCTCTCTTAA+AGG + Chr1:76355794-76355813 None:intergenic 40.0%
AAGCCGCATACTGAGTATTT+GGG + Chr1:76356169-76356188 None:intergenic 40.0%
AAGGGTCCTATAATTAGGGA+CGG + Chr1:76355692-76355711 None:intergenic 40.0%
AATAAAAGTAGTACCAGGGC+CGG - Chr1:76354782-76354801 MsG0180004314.01.T01:intron 40.0%
ACCTATGGATTTATCCGTTG+GGG - Chr1:76356232-76356251 MsG0180004314.01.T01:intron 40.0%
ACTGTGAGATGATAGAGTCA+AGG + Chr1:76354055-76354074 None:intergenic 40.0%
ATACTGAGTATTTGGGGACA+AGG + Chr1:76356162-76356181 None:intergenic 40.0%
CTATCTCCGTCCCTAATTAT+AGG - Chr1:76355683-76355702 MsG0180004314.01.T01:intron 40.0%
CTCACCATTAATTGCAGTGA+TGG - Chr1:76354434-76354453 MsG0180004314.01.T01:intron 40.0%
GAACCTATGGATTTATCCGT+TGG - Chr1:76356230-76356249 MsG0180004314.01.T01:intron 40.0%
GACCATGATCGATGATGATA+GGG + Chr1:76354136-76354155 None:intergenic 40.0%
GAGGGAATAAAAGTAGTACC+AGG - Chr1:76354777-76354796 MsG0180004314.01.T01:intron 40.0%
GCGCCAATTTCTCTCATATT+AGG - Chr1:76354237-76354256 MsG0180004314.01.T01:CDS 40.0%
GTATAGAATGACCTAGTGCT+TGG + Chr1:76355112-76355131 None:intergenic 40.0%
GTGGAATGACTTTCAAACGA+TGG + Chr1:76354936-76354955 None:intergenic 40.0%
GTGTACTCACTAGAAATTCG+TGG - Chr1:76356119-76356138 MsG0180004314.01.T01:intron 40.0%
GTTAATGAAAACATGTCGGG+TGG + Chr1:76354610-76354629 None:intergenic 40.0%
TAAGCCGCATACTGAGTATT+TGG + Chr1:76356170-76356189 None:intergenic 40.0%
TTCGGACCCCAAAATATTTG+AGG + Chr1:76354844-76354863 None:intergenic 40.0%
! AAGATAGAGAGCCTTTTTGG+TGG + Chr1:76354099-76354118 None:intergenic 40.0%
! AGCAAGATAGAGAGCCTTTT+TGG + Chr1:76354102-76354121 None:intergenic 40.0%
! AGGGTCTTATAATTAGGGAC+GGG - Chr1:76355950-76355969 MsG0180004314.01.T01:CDS 40.0%
! ATATTGTAGGTTGGGATCAG+GGG - Chr1:76355209-76355228 MsG0180004314.01.T01:intron 40.0%
! GCACAGTGCCTCAAATATTT+TGG - Chr1:76354833-76354852 MsG0180004314.01.T01:intron 40.0%
! GCTTTTCATGAATTGACGGA+AGG - Chr1:76353912-76353931 MsG0180004314.01.T01:CDS 40.0%
! GGCAGCTTTTCATGAATTGA+CGG - Chr1:76353908-76353927 MsG0180004314.01.T01:CDS 40.0%
! TATCCTTTAAGAGAGAGGGT+TGG - Chr1:76355788-76355807 MsG0180004314.01.T01:intron 40.0%
!! AGATAGAGAGCCTTTTTGGT+GGG + Chr1:76354098-76354117 None:intergenic 40.0%
AAAACATGTCGGGTGGTAAC+AGG + Chr1:76354603-76354622 None:intergenic 45.0%
AATGGCAAACCGCGAAAGAA+TGG - Chr1:76353884-76353903 MsG0180004314.01.T01:CDS 45.0%
ACCCCAACGGATAAATCCAT+AGG + Chr1:76356236-76356255 None:intergenic 45.0%
AGCCGCATACTGAGTATTTG+GGG + Chr1:76356168-76356187 None:intergenic 45.0%
AGTCAGAGGAGTCAAGTACA+AGG - Chr1:76355249-76355268 MsG0180004314.01.T01:intron 45.0%
ATATGAGAGAAATTGGCGCG+CGG + Chr1:76354236-76354255 None:intergenic 45.0%
CATCATCGATCATGGTCGAA+AGG - Chr1:76354139-76354158 MsG0180004314.01.T01:CDS 45.0%
CGACCATGATCGATGATGAT+AGG + Chr1:76354137-76354156 None:intergenic 45.0%
GAAACGGAAGTGGTTCCAAA+TGG + Chr1:76354033-76354052 None:intergenic 45.0%
GAAGTGGTTCCAAATGGTGT+CGG + Chr1:76354027-76354046 None:intergenic 45.0%
GATTTATCCGTTGGGGTTGA+AGG - Chr1:76356239-76356258 MsG0180004314.01.T01:intron 45.0%
GCTAACCTGGAATCATGCAT+AGG + Chr1:76354509-76354528 None:intergenic 45.0%
GGATTCCTATGCATGATTCC+AGG - Chr1:76354501-76354520 MsG0180004314.01.T01:intron 45.0%
GTCCCCAAATACTCAGTATG+CGG - Chr1:76356163-76356182 MsG0180004314.01.T01:intron 45.0%
GTCCTAGTGTGAGAAGTGAT+GGG - Chr1:76356393-76356412 MsG0180004314.01.T01:CDS 45.0%
TAGAGTCAAGGAAACGGAAG+TGG + Chr1:76354043-76354062 None:intergenic 45.0%
TATGAGACAGAATGGCAGAG+AGG + Chr1:76353941-76353960 None:intergenic 45.0%
! AGGCCGCGTAACAAAGTATT+AGG - Chr1:76356362-76356381 MsG0180004314.01.T01:CDS 45.0%
! GGGTCTTATAATTAGGGACG+GGG - Chr1:76355951-76355970 MsG0180004314.01.T01:CDS 45.0%
! GTTCCAAATGGTGTCGGAAT+TGG + Chr1:76354021-76354040 None:intergenic 45.0%
!! TGCCTCTAGCCTAGTCTTAA+CGG + Chr1:76354816-76354835 None:intergenic 45.0%
!! AAAAAAATGTATAATAAATA+GGG - Chr1:76355848-76355867 MsG0180004314.01.T01:intron 5.0%
!! AAAAATAAAAAAAAGTTATT+CGG + Chr1:76354983-76355002 None:intergenic 5.0%
!!! ATAAAATATATATTAGTTTA+GGG + Chr1:76355352-76355371 None:intergenic 5.0%
!!! TATAAAATATATATTAGTTT+AGG + Chr1:76355353-76355372 None:intergenic 5.0%
AGTCTTAACGGCCTATGGAC+CGG + Chr1:76354804-76354823 None:intergenic 50.0%
AGTTCCATCATCATGCGAGG+AGG - Chr1:76356342-76356361 MsG0180004314.01.T01:CDS 50.0%
ATGTCCACCTTCAACCCCAA+CGG + Chr1:76356249-76356268 None:intergenic 50.0%
CACCCATCACTTCTCACACT+AGG + Chr1:76356398-76356417 None:intergenic 50.0%
CGCCCTATCATCATCGATCA+TGG - Chr1:76354131-76354150 MsG0180004314.01.T01:CDS 50.0%
CGTCCTAGTGTGAGAAGTGA+TGG - Chr1:76356392-76356411 MsG0180004314.01.T01:CDS 50.0%
CTGCCAATTCCGACACCATT+TGG - Chr1:76354015-76354034 MsG0180004314.01.T01:CDS 50.0%
CTTGCATGACACGATCGTCT+CGG + Chr1:76356301-76356320 None:intergenic 50.0%
GATAGCCTGCAAACGTCAAC+GGG + Chr1:76353969-76353988 None:intergenic 50.0%
GGATAGCCTGCAAACGTCAA+CGG + Chr1:76353970-76353989 None:intergenic 50.0%
GGGAGTGGTATGAGACAGAA+TGG + Chr1:76353949-76353968 None:intergenic 50.0%
GGGTGGTTGGAGATTGAGAT+GGG - Chr1:76356413-76356432 MsG0180004314.01.T01:CDS 50.0%
GTCAGTTCCATCATCATGCG+AGG - Chr1:76356339-76356358 MsG0180004314.01.T01:CDS 50.0%
GTCCATAGGCCGTTAAGACT+AGG - Chr1:76354804-76354823 MsG0180004314.01.T01:intron 50.0%
TGGGTGGTTGGAGATTGAGA+TGG - Chr1:76356412-76356431 MsG0180004314.01.T01:CDS 50.0%
TGTCGGAATTGGCAGCAGAA+AGG + Chr1:76354010-76354029 None:intergenic 50.0%
TTATCCGTTGGGGTTGAAGG+TGG - Chr1:76356242-76356261 MsG0180004314.01.T01:intron 50.0%
TTCGCGGTTTGCCATTCTTC+TGG + Chr1:76353880-76353899 None:intergenic 50.0%
! CTAGTGTGAGAAGTGATGGG+TGG - Chr1:76356396-76356415 MsG0180004314.01.T01:CDS 50.0%
!! AGCCTAGTCTTAACGGCCTA+TGG + Chr1:76354809-76354828 None:intergenic 50.0%
!! TGTGAGAAGTGATGGGTGGT+TGG - Chr1:76356400-76356419 MsG0180004314.01.T01:CDS 50.0%
AAACCAGCGATGGAGGGAGA+GGG + Chr1:76355434-76355453 None:intergenic 55.0%
AAGCTGCCGCCATTCTTTCG+CGG + Chr1:76353896-76353915 None:intergenic 55.0%
AGGTTCCAAACCAGCGATGG+AGG + Chr1:76355441-76355460 None:intergenic 55.0%
GAGAGGTTCCAAACCAGCGA+TGG + Chr1:76355444-76355463 None:intergenic 55.0%
GGCAAACCGCGAAAGAATGG+CGG - Chr1:76353887-76353906 MsG0180004314.01.T01:CDS 55.0%
GGCCGTTAAGACTAGGCTAG+AGG - Chr1:76354811-76354830 MsG0180004314.01.T01:intron 55.0%
GGTTCCAAACCAGCGATGGA+GGG + Chr1:76355440-76355459 None:intergenic 55.0%
TGCCAACGCTCCCACCAAAA+AGG - Chr1:76354085-76354104 MsG0180004314.01.T01:CDS 55.0%
! TGTAGGTTGGGATCAGGGGT+GGG - Chr1:76355213-76355232 MsG0180004314.01.T01:intron 55.0%
! TTGTAGGTTGGGATCAGGGG+TGG - Chr1:76355212-76355231 MsG0180004314.01.T01:intron 55.0%
!! AGCCTTTTTGGTGGGAGCGT+TGG + Chr1:76354090-76354109 None:intergenic 55.0%
!! TTTGGTGGGAGCGTTGGCAA+GGG + Chr1:76354084-76354103 None:intergenic 55.0%
!!! TTTTGGTGGGAGCGTTGGCA+AGG + Chr1:76354085-76354104 None:intergenic 55.0%
AACCAGCGATGGAGGGAGAG+GGG + Chr1:76355433-76355452 None:intergenic 60.0%
CAAACCAGCGATGGAGGGAG+AGG + Chr1:76355435-76355454 None:intergenic 60.0%
CCACTCCCGTTGACGTTTGC+AGG - Chr1:76353961-76353980 MsG0180004314.01.T01:CDS 60.0%
CCTGCAAACGTCAACGGGAG+TGG + Chr1:76353964-76353983 None:intergenic 60.0%
GGGAGAGGGGAGGACTTTAG+AGG + Chr1:76355420-76355439 None:intergenic 60.0%
TAGTACCAGGGCCGGTCCAT+AGG - Chr1:76354790-76354809 MsG0180004314.01.T01:intron 60.0%
! CTCTCCCTCCATCGCTGGTT+TGG - Chr1:76355433-76355452 MsG0180004314.01.T01:intron 60.0%
! GTAGGTTGGGATCAGGGGTG+GGG - Chr1:76355214-76355233 MsG0180004314.01.T01:intron 60.0%
GCGGCCTCCTCGCATGATGA+TGG + Chr1:76356349-76356368 None:intergenic 65.0%
!! GTGGGAGCGTTGGCAAGGGA+AGG + Chr1:76354080-76354099 None:intergenic 65.0%
AACGGCCTATGGACCGGCCC+TGG + Chr1:76354798-76354817 None:intergenic 70.0%
CAGCGATGGAGGGAGAGGGG+AGG + Chr1:76355430-76355449 None:intergenic 70.0%
CTCCCCTCTCCCTCCATCGC+TGG - Chr1:76355428-76355447 MsG0180004314.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 76353867 76356565 76353867 ID=MsG0180004314.01;Name=MsG0180004314.01
Chr1 mRNA 76353867 76356565 76353867 ID=MsG0180004314.01.T01;Parent=MsG0180004314.01;Name=MsG0180004314.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|304
Chr1 exon 76356272 76356565 76356272 ID=MsG0180004314.01.T01:exon:12631;Parent=MsG0180004314.01.T01
Chr1 exon 76355910 76356055 76355910 ID=MsG0180004314.01.T01:exon:12630;Parent=MsG0180004314.01.T01
Chr1 exon 76353867 76354341 76353867 ID=MsG0180004314.01.T01:exon:12629;Parent=MsG0180004314.01.T01
Chr1 CDS 76356272 76356565 76356272 ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01
Chr1 CDS 76355910 76356055 76355910 ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01
Chr1 CDS 76353867 76354341 76353867 ID=MsG0180004314.01.T01:cds;Parent=MsG0180004314.01.T01
Gene Sequence

>MsG0180004314.01.T01

ATGGCAAAAACCAGAAGAATGGCAAACCGCGAAAGAATGGCGGCAGCTTTTCATGAATTGACGGAAGGATGCCTCTCTGCCATTCTGTCTCATACCACTCCCGTTGACGTTTGCAGGCTATCCGTTGTTTCTAAGATTTTCCTTTCTGCTGCCAATTCCGACACCATTTGGAACCACTTCCGTTTCCTTGACTCTATCATCTCACAGTCTCCTTCCCTTGCCAACGCTCCCACCAAAAAGGCTCTCTATCTTGCTCTCTCTGATCGCCCTATCATCATCGATCATGGTCGAAAGAGCTTTCAATTGGATAGGAAGAGTGGAAAAATATGTTACATGCGTGCTGCTAAATCTCTCACCATTAATTGCAGTGATGGTGATAGTGATGAAGATGATGATGAGTTGCTTGTTAAGAAAGAGTGGATTCCTATGCATGATTCCAGGTTTCCTGAAGTTTCTAAGCTTTGTCTTGTGTACTCACTAGAAATTCGTGGGATGATTAACACTCTTTCCTTGTCCCCAAATACTCAGTATGCGGCTTATCTCGTGTTCAAGATGATTAATGCCTATGGATTTGAGAATGAACCTATGGATTTATCCGTTGGGGTTGAAGGTGGACATAGCAGCACTAAATCTGTCTATTTGCACAACCGAGACGATCGTGTCATGCAAGCAAGTTGTAAATGTGGTCGTCAGTTCCATCATCATGCGAGGAGGCCGCGTAACAAAGTATTAGGATTGCAACGTCCTAGTGTGAGAAGTGATGGGTGGTTGGAGATTGAGATGGGAGAGTTCTTCAATTCAAGTCTTGAAGATGAAGAAATCCAAATGAGTGTTGTCGAGAAATTTGATAGTGGCAATGAGAAGGGAAATTTCTATCTTGAAGGAATAGAAGTTAGGCTTAAGAAAGACAAGTAA
Protein sequence

>MsG0180004314.01.T01

MAKTRRMANRERMAAAFHELTEGCLSAILSHTTPVDVCRLSVVSKIFLSAANSDTIWNHFRFLDSIISQSPSLANAPTKKALYLALSDRPIIIDHGRKSFQLDRKSGKICYMRAAKSLTINCSDGDSDEDDDELLVKKEWIPMHDSRFPEVSKLCLVYSLEIRGMINTLSLSPNTQYAAYLVFKMINAYGFENEPMDLSVGVEGGHSSTKSVYLHNRDDRVMQASCKCGRQFHHHARRPRNKVLGLQRPSVRSDGWLEIEMGEFFNSSLEDEEIQMSVVEKFDSGNEKGNFYLEGIEVRLKKDK*