Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004336.01.T01 | XP_013468822.1 | 100 | 164 | 0 | 0 | 1 | 164 | 74 | 237 | 9.89E-114 | 332 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004336.01.T01 | A0A072VLL2 | 100.000 | 164 | 0 | 0 | 1 | 164 | 74 | 237 | 4.72e-114 | 332 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004211.01 | MsG0180004336.01 | 0.996048 | 8.473452e-223 | 1.440121e-215 |
| MsG0180004336.01 | MsG0380017667.01 | 0.801190 | 1.012210e-48 | 4.803792e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004336.01.T01 | MTR_1g077210 | 100.000 | 164 | 0 | 0 | 1 | 164 | 74 | 237 | 1.20e-117 | 332 |
| MsG0180004336.01.T01 | MTR_3g109280 | 78.523 | 149 | 30 | 1 | 1 | 149 | 74 | 220 | 4.29e-81 | 239 |
| MsG0180004336.01.T01 | MTR_0054s0080 | 73.171 | 164 | 39 | 3 | 1 | 164 | 74 | 232 | 6.94e-81 | 239 |
| MsG0180004336.01.T01 | MTR_1g009030 | 67.683 | 164 | 33 | 2 | 1 | 164 | 74 | 217 | 1.13e-70 | 213 |
| MsG0180004336.01.T01 | MTR_6g066040 | 59.211 | 152 | 60 | 1 | 1 | 152 | 74 | 223 | 1.93e-57 | 179 |
| MsG0180004336.01.T01 | MTR_6g066090 | 59.589 | 146 | 57 | 1 | 1 | 146 | 1 | 144 | 1.00e-56 | 175 |
| MsG0180004336.01.T01 | MTR_5g068640 | 34.868 | 152 | 92 | 3 | 1 | 146 | 72 | 222 | 9.78e-24 | 93.6 |
| MsG0180004336.01.T01 | MTR_8g106520 | 35.211 | 142 | 85 | 2 | 1 | 142 | 16 | 150 | 4.73e-19 | 79.7 |
| MsG0180004336.01.T01 | MTR_8g106520 | 35.211 | 142 | 85 | 2 | 1 | 142 | 72 | 206 | 2.50e-18 | 79.0 |
| MsG0180004336.01.T01 | MTR_7g073370 | 38.532 | 109 | 62 | 1 | 36 | 144 | 108 | 211 | 1.73e-17 | 76.6 |
| MsG0180004336.01.T01 | MTR_2g014820 | 35.915 | 142 | 81 | 4 | 2 | 141 | 718 | 851 | 1.53e-14 | 70.5 |
| MsG0180004336.01.T01 | MTR_8g106530 | 33.562 | 146 | 81 | 4 | 1 | 142 | 329 | 462 | 1.14e-12 | 65.1 |
| MsG0180004336.01.T01 | MTR_8g106530 | 31.944 | 144 | 87 | 3 | 1 | 142 | 73 | 207 | 1.61e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004336.01.T01 | AT4G31330 | 72.109 | 147 | 37 | 2 | 1 | 143 | 74 | 220 | 1.00e-70 | 213 |
| MsG0180004336.01.T01 | AT5G10580 | 64.327 | 171 | 53 | 2 | 1 | 163 | 74 | 244 | 1.08e-69 | 211 |
| MsG0180004336.01.T01 | AT5G10580 | 64.327 | 171 | 53 | 2 | 1 | 163 | 74 | 244 | 1.08e-69 | 211 |
| MsG0180004336.01.T01 | AT5G10580 | 70.000 | 150 | 39 | 1 | 1 | 144 | 74 | 223 | 1.61e-68 | 208 |
| MsG0180004336.01.T01 | AT5G24790 | 56.875 | 160 | 66 | 1 | 1 | 157 | 74 | 233 | 2.06e-50 | 162 |
| MsG0180004336.01.T01 | AT5G10580 | 66.071 | 112 | 32 | 1 | 1 | 106 | 74 | 185 | 5.48e-45 | 146 |
| MsG0180004336.01.T01 | AT3G18215 | 37.324 | 142 | 87 | 1 | 1 | 142 | 1 | 140 | 4.25e-21 | 85.1 |
| MsG0180004336.01.T01 | AT3G18215 | 37.324 | 142 | 87 | 1 | 1 | 142 | 73 | 212 | 2.61e-20 | 84.7 |
| MsG0180004336.01.T01 | AT5G24600 | 30.263 | 152 | 94 | 3 | 1 | 142 | 72 | 221 | 2.18e-16 | 74.3 |
| MsG0180004336.01.T01 | AT5G24600 | 30.263 | 152 | 94 | 3 | 1 | 142 | 72 | 221 | 2.19e-16 | 73.9 |
Find 27 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTCAATGATTCCATTTA+TGG | 0.062471 | 1:+76569589 | MsG0180004336.01.T01:CDS |
| GATTCCAGTGCTTTATAATT+TGG | 0.112917 | 1:+76569903 | MsG0180004336.01.T01:CDS |
| TGAATATTTGTCTGAGCTTT+TGG | 0.189699 | 1:+76569771 | MsG0180004336.01.T01:CDS |
| TGGAGCTCATAGTGAATTTA+TGG | 0.212693 | 1:+76569609 | MsG0180004336.01.T01:CDS |
| TTCACTATGAGCTCCATAAA+TGG | 0.279592 | 1:-76569602 | None:intergenic |
| CATCTTGTGGTGTGCAAATA+AGG | 0.279736 | 1:-76569723 | None:intergenic |
| TCTGTCACACATTGTCCATT+AGG | 0.321977 | 1:+76569680 | MsG0180004336.01.T01:CDS |
| AATAATGGGATCAACACTTA+TGG | 0.371912 | 1:+76569498 | None:intergenic |
| CATTCTACTCTCAGCTGGTT+TGG | 0.383869 | 1:+76569531 | MsG0180004336.01.T01:CDS |
| TTGGACTTTGTTTGTGGGAA+TGG | 0.387422 | 1:+76569922 | MsG0180004336.01.T01:CDS |
| TATAATTTGGACTTTGTTTG+TGG | 0.396446 | 1:+76569916 | MsG0180004336.01.T01:CDS |
| TTGGATCTTTGGACCTGTCT+TGG | 0.400498 | 1:+76569858 | MsG0180004336.01.T01:CDS |
| CCAAAGATCCAAAGCAAAAG+AGG | 0.411193 | 1:-76569847 | None:intergenic |
| AAAAGCTCAGACAAATATTC+AGG | 0.422507 | 1:-76569769 | None:intergenic |
| ATAATTTGGACTTTGTTTGT+GGG | 0.446470 | 1:+76569917 | MsG0180004336.01.T01:CDS |
| ATGAGAATAAGAATATAGTT+AGG | 0.446792 | 1:-76569654 | None:intergenic |
| TGCAAATAAGGATGCTCACT+TGG | 0.496404 | 1:-76569711 | None:intergenic |
| ACATCCATTCTACTCTCAGC+TGG | 0.523277 | 1:+76569526 | MsG0180004336.01.T01:CDS |
| AAGTCCAAATTATAAAGCAC+TGG | 0.539896 | 1:-76569907 | None:intergenic |
| GAACACAAGAAAACCAAGAC+AGG | 0.547850 | 1:-76569871 | None:intergenic |
| CAAACCAGCTGAGAGTAGAA+TGG | 0.570136 | 1:-76569530 | None:intergenic |
| GTGAAGATTAATGACAAAGG+AGG | 0.586414 | 1:+76569958 | MsG0180004336.01.T01:CDS |
| CAAAGATCCAAAGCAAAAGA+GGG | 0.615516 | 1:-76569846 | None:intergenic |
| GCATAGGACAAGACATCTTG+TGG | 0.640111 | 1:-76569736 | None:intergenic |
| CCATAAATGGAATCATTGAG+AGG | 0.651845 | 1:-76569589 | None:intergenic |
| ATTGTGAAGATTAATGACAA+AGG | 0.655961 | 1:+76569955 | MsG0180004336.01.T01:CDS |
| TTCAGGTGTGATGACAGCAT+AGG | 0.682375 | 1:-76569752 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGAATAAGAATATAGTT+AGG | - | Chr1:76569657-76569676 | None:intergenic | 20.0% |
| ! | ATTGTGAAGATTAATGACAA+AGG | + | Chr1:76569955-76569974 | MsG0180004336.01.T01:CDS | 25.0% |
| !! | ATAATTTGGACTTTGTTTGT+GGG | + | Chr1:76569917-76569936 | MsG0180004336.01.T01:CDS | 25.0% |
| !! | TATAATTTGGACTTTGTTTG+TGG | + | Chr1:76569916-76569935 | MsG0180004336.01.T01:CDS | 25.0% |
| AAAAGCTCAGACAAATATTC+AGG | - | Chr1:76569772-76569791 | None:intergenic | 30.0% | |
| AAGTCCAAATTATAAAGCAC+TGG | - | Chr1:76569910-76569929 | None:intergenic | 30.0% | |
| GATTCCAGTGCTTTATAATT+TGG | + | Chr1:76569903-76569922 | MsG0180004336.01.T01:CDS | 30.0% | |
| ! | TGAATATTTGTCTGAGCTTT+TGG | + | Chr1:76569771-76569790 | MsG0180004336.01.T01:CDS | 30.0% |
| !! | TAGGAGTGTGACATATTTTA+GGG | - | Chr1:76569638-76569657 | None:intergenic | 30.0% |
| !! | TTAGGAGTGTGACATATTTT+AGG | - | Chr1:76569639-76569658 | None:intergenic | 30.0% |
| CAAAGATCCAAAGCAAAAGA+GGG | - | Chr1:76569849-76569868 | None:intergenic | 35.0% | |
| CACTTGGTTGAAAAACCTAA+TGG | - | Chr1:76569698-76569717 | None:intergenic | 35.0% | |
| CCATAAATGGAATCATTGAG+AGG | - | Chr1:76569592-76569611 | None:intergenic | 35.0% | |
| CCTCTCAATGATTCCATTTA+TGG | + | Chr1:76569589-76569608 | MsG0180004336.01.T01:CDS | 35.0% | |
| GTGAAGATTAATGACAAAGG+AGG | + | Chr1:76569958-76569977 | MsG0180004336.01.T01:CDS | 35.0% | |
| TGGAGCTCATAGTGAATTTA+TGG | + | Chr1:76569609-76569628 | MsG0180004336.01.T01:CDS | 35.0% | |
| TTCACTATGAGCTCCATAAA+TGG | - | Chr1:76569605-76569624 | None:intergenic | 35.0% | |
| CATCTTGTGGTGTGCAAATA+AGG | - | Chr1:76569726-76569745 | None:intergenic | 40.0% | |
| CCAAAGATCCAAAGCAAAAG+AGG | - | Chr1:76569850-76569869 | None:intergenic | 40.0% | |
| GAACACAAGAAAACCAAGAC+AGG | - | Chr1:76569874-76569893 | None:intergenic | 40.0% | |
| TCTGTCACACATTGTCCATT+AGG | + | Chr1:76569680-76569699 | MsG0180004336.01.T01:CDS | 40.0% | |
| TGCAAATAAGGATGCTCACT+TGG | - | Chr1:76569714-76569733 | None:intergenic | 40.0% | |
| ! | TTGGACTTTGTTTGTGGGAA+TGG | + | Chr1:76569922-76569941 | MsG0180004336.01.T01:CDS | 40.0% |
| ! | TTGTCTGAGCTTTTGGACAA+AGG | + | Chr1:76569778-76569797 | MsG0180004336.01.T01:CDS | 40.0% |
| !! | CCTCTTTTGCTTTGGATCTT+TGG | + | Chr1:76569847-76569866 | MsG0180004336.01.T01:CDS | 40.0% |
| !!! | GGAATTGTTTTGAGCACTGT+TGG | + | Chr1:76569799-76569818 | MsG0180004336.01.T01:CDS | 40.0% |
| ACATCCATTCTACTCTCAGC+TGG | + | Chr1:76569526-76569545 | MsG0180004336.01.T01:CDS | 45.0% | |
| CAAACCAGCTGAGAGTAGAA+TGG | - | Chr1:76569533-76569552 | None:intergenic | 45.0% | |
| CATTCTACTCTCAGCTGGTT+TGG | + | Chr1:76569531-76569550 | MsG0180004336.01.T01:CDS | 45.0% | |
| GCATAGGACAAGACATCTTG+TGG | - | Chr1:76569739-76569758 | None:intergenic | 45.0% | |
| TTCAGGTGTGATGACAGCAT+AGG | - | Chr1:76569755-76569774 | None:intergenic | 45.0% | |
| TTGGATCTTTGGACCTGTCT+TGG | + | Chr1:76569858-76569877 | MsG0180004336.01.T01:CDS | 45.0% | |
| ! | CAGCATTCCCTCTTTTGCTT+TGG | + | Chr1:76569839-76569858 | MsG0180004336.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 76569502 | 76569996 | 76569502 | ID=MsG0180004336.01;Name=MsG0180004336.01 |
| Chr1 | mRNA | 76569502 | 76569996 | 76569502 | ID=MsG0180004336.01.T01;Parent=MsG0180004336.01;Name=MsG0180004336.01.T01;_AED=0.20;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|164 |
| Chr1 | exon | 76569502 | 76569996 | 76569502 | ID=MsG0180004336.01.T01:exon:44912;Parent=MsG0180004336.01.T01 |
| Chr1 | CDS | 76569502 | 76569996 | 76569502 | ID=MsG0180004336.01.T01:cds;Parent=MsG0180004336.01.T01 |
| Gene Sequence |
| Protein sequence |
