Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004354.01.T01 | XP_013468844.1 | 97.6 | 250 | 6 | 0 | 1 | 250 | 1 | 250 | 1.05E-178 | 501 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004354.01.T01 | Q8LD98 | 48.638 | 257 | 120 | 6 | 1 | 250 | 1 | 252 | 1.16E-72 | 225 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004354.01.T01 | A0A072VMR2 | 97.600 | 250 | 6 | 0 | 1 | 250 | 1 | 250 | 5.03e-179 | 501 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004354.01 | MsG0280010173.01 | 0.819780 | 9.703136e-53 | 7.538758e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004354.01.T01 | MTR_1g077560 | 97.600 | 250 | 6 | 0 | 1 | 250 | 1 | 250 | 0.0 | 501 |
| MsG0180004354.01.T01 | MTR_3g063310 | 50.000 | 254 | 120 | 5 | 1 | 250 | 2 | 252 | 1.70e-70 | 217 |
| MsG0180004354.01.T01 | MTR_5g076170 | 47.547 | 265 | 122 | 8 | 1 | 250 | 1 | 263 | 8.55e-68 | 210 |
| MsG0180004354.01.T01 | MTR_5g087760 | 33.333 | 222 | 142 | 3 | 33 | 250 | 35 | 254 | 7.56e-41 | 141 |
| MsG0180004354.01.T01 | MTR_2g012550 | 34.872 | 195 | 118 | 5 | 55 | 245 | 69 | 258 | 3.15e-34 | 124 |
| MsG0180004354.01.T01 | MTR_3g078800 | 36.559 | 186 | 114 | 3 | 67 | 250 | 69 | 252 | 4.33e-34 | 124 |
| MsG0180004354.01.T01 | MTR_3g078810 | 38.525 | 122 | 71 | 3 | 114 | 232 | 9 | 129 | 8.25e-21 | 85.9 |
| MsG0180004354.01.T01 | MTR_3g078820 | 32.000 | 150 | 100 | 2 | 85 | 233 | 43 | 191 | 1.31e-18 | 81.6 |
| MsG0180004354.01.T01 | MTR_2g090225 | 34.054 | 185 | 119 | 3 | 52 | 234 | 21 | 204 | 1.19e-17 | 79.3 |
| MsG0180004354.01.T01 | MTR_3g074150 | 26.570 | 207 | 137 | 6 | 33 | 235 | 37 | 232 | 4.58e-17 | 78.6 |
| MsG0180004354.01.T01 | MTR_2g011350 | 31.288 | 163 | 108 | 3 | 87 | 248 | 43 | 202 | 8.52e-17 | 76.6 |
| MsG0180004354.01.T01 | MTR_2g100060 | 30.000 | 150 | 102 | 2 | 80 | 227 | 77 | 225 | 1.47e-16 | 77.0 |
| MsG0180004354.01.T01 | MTR_3g104460 | 25.000 | 200 | 135 | 5 | 62 | 250 | 58 | 253 | 6.55e-14 | 69.7 |
| MsG0180004354.01.T01 | MTR_1g057270 | 25.146 | 171 | 121 | 4 | 86 | 250 | 75 | 244 | 2.01e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004354.01.T01 | AT5G36970 | 51.779 | 253 | 114 | 5 | 1 | 250 | 1 | 248 | 4.91e-84 | 251 |
| MsG0180004354.01.T01 | AT1G54540 | 52.590 | 251 | 106 | 4 | 1 | 250 | 1 | 239 | 6.49e-76 | 230 |
| MsG0180004354.01.T01 | AT1G65690 | 48.638 | 257 | 120 | 6 | 1 | 250 | 1 | 252 | 1.19e-73 | 225 |
| MsG0180004354.01.T01 | AT2G27080 | 31.535 | 241 | 150 | 6 | 13 | 243 | 20 | 255 | 2.60e-35 | 127 |
| MsG0180004354.01.T01 | AT2G27080 | 31.535 | 241 | 150 | 6 | 13 | 243 | 20 | 255 | 2.60e-35 | 127 |
| MsG0180004354.01.T01 | AT5G21130 | 27.888 | 251 | 159 | 5 | 5 | 250 | 47 | 280 | 9.38e-31 | 115 |
| MsG0180004354.01.T01 | AT3G11650 | 26.577 | 222 | 150 | 6 | 39 | 248 | 19 | 239 | 7.23e-18 | 80.5 |
| MsG0180004354.01.T01 | AT5G06320 | 29.480 | 173 | 113 | 5 | 69 | 234 | 45 | 215 | 6.64e-14 | 69.3 |
| MsG0180004354.01.T01 | AT2G22180 | 26.872 | 227 | 133 | 8 | 33 | 249 | 87 | 290 | 3.09e-13 | 68.2 |
| MsG0180004354.01.T01 | AT2G35460 | 24.365 | 197 | 136 | 6 | 59 | 248 | 48 | 238 | 5.22e-13 | 67.0 |
| MsG0180004354.01.T01 | AT5G53730 | 26.404 | 178 | 120 | 4 | 74 | 245 | 38 | 210 | 5.32e-11 | 60.8 |
Find 49 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACCAATTGCTATCAAATT+TGG | 0.094068 | 1:+76817157 | MsG0180004354.01.T01:CDS |
| TGATGACCTTGGTAGAATTT+AGG | 0.230469 | 1:-76817016 | None:intergenic |
| TAAATACCAATCTTCTTGTT+TGG | 0.277530 | 1:-76816929 | None:intergenic |
| GTTGAAGAAGGTGAAAGTTT+TGG | 0.278918 | 1:+76817189 | MsG0180004354.01.T01:CDS |
| CAGTGTGTCAACTGAGTAAT+CGG | 0.327502 | 1:-76816831 | None:intergenic |
| AAATACCAATCTTCTTGTTT+GGG | 0.328060 | 1:-76816928 | None:intergenic |
| CACCAATTGCTATCAAATTT+GGG | 0.342744 | 1:+76817158 | MsG0180004354.01.T01:CDS |
| GTGCTTAATGTGTCATTAAC+AGG | 0.356026 | 1:+76817049 | MsG0180004354.01.T01:CDS |
| CTCCCAAATTTGATAGCAAT+TGG | 0.360757 | 1:-76817160 | None:intergenic |
| ACTGAGTAATCGGGTAGCTT+TGG | 0.432738 | 1:-76816821 | None:intergenic |
| ACCAATCTTCTTGTTTGGGT+TGG | 0.457491 | 1:-76816924 | None:intergenic |
| ATTGGTATTTACTATGAGAA+AGG | 0.464515 | 1:+76816941 | MsG0180004354.01.T01:CDS |
| TGGTTTGAAGATGAGGTAAA+GGG | 0.469576 | 1:-76816801 | None:intergenic |
| GGAGGTACCAATGGTGTCCT+AGG | 0.470142 | 1:-76816605 | None:intergenic |
| TGGGAGATTGAAGTTGAAGA+AGG | 0.472195 | 1:+76817177 | MsG0180004354.01.T01:CDS |
| ATTATCATTGCTGCAACTGC+AGG | 0.473517 | 1:+76816776 | MsG0180004354.01.T01:CDS |
| TTGGTTTGAAGATGAGGTAA+AGG | 0.475046 | 1:-76816802 | None:intergenic |
| ACCAACCCAAACAAGAAGAT+TGG | 0.497061 | 1:+76816923 | MsG0180004354.01.T01:CDS |
| CAGCAATGATAATGAGAAGA+AGG | 0.497956 | 1:-76816766 | None:intergenic |
| TTGCAGCAACAACAGCAAAC+AGG | 0.499589 | 1:+76817106 | MsG0180004354.01.T01:CDS |
| AGGTTCCTCTAGATCAGAAA+AGG | 0.515504 | 1:+76816628 | MsG0180004354.01.T01:CDS |
| ACCAATGGTGTCCTAGGATG+TGG | 0.520057 | 1:-76816599 | None:intergenic |
| TGGAAACAAAACCACATCCT+AGG | 0.526613 | 1:+76816588 | MsG0180004354.01.T01:CDS |
| GGTATTTACTATGAGAAAGG+AGG | 0.530926 | 1:+76816944 | MsG0180004354.01.T01:CDS |
| TTAACAGGTCAAGTTCAGTC+TGG | 0.538536 | 1:+76817064 | MsG0180004354.01.T01:CDS |
| TTGTTTCCACATTCATAGGG+TGG | 0.541657 | 1:-76816574 | None:intergenic |
| TTTGGGCCGATGTCAGTTGG+TGG | 0.542429 | 1:+76817207 | MsG0180004354.01.T01:CDS |
| ACTGCATCAACATGCTATGC+CGG | 0.545439 | 1:+76816673 | MsG0180004354.01.T01:CDS |
| GTAGCTTTGGTTTGAAGATG+AGG | 0.546427 | 1:-76816808 | None:intergenic |
| GTATTTACTATGAGAAAGGA+GGG | 0.550794 | 1:+76816945 | MsG0180004354.01.T01:CDS |
| AGGACACCATTGGTACCTCC+AGG | 0.562296 | 1:+76816608 | MsG0180004354.01.T01:CDS |
| GTCTGGAAACTCGTTAATGA+CGG | 0.566450 | 1:+76817081 | MsG0180004354.01.T01:CDS |
| ATTTGATAGCAATTGGTGCA+TGG | 0.567519 | 1:-76817153 | None:intergenic |
| TAATAATCTTGTAAGCATCA+AGG | 0.573221 | 1:+76817246 | MsG0180004354.01.T01:CDS |
| TGTTGATGCAGTAGTGGAGG+AGG | 0.581380 | 1:-76816662 | None:intergenic |
| GAGAATCCACCCTATGAATG+TGG | 0.585292 | 1:+76816568 | MsG0180004354.01.T01:CDS |
| GCAAACAGGAAGAATTCCGT+TGG | 0.588670 | 1:+76817120 | MsG0180004354.01.T01:CDS |
| GAGGAACCTGGAGGTACCAA+TGG | 0.601267 | 1:-76816614 | None:intergenic |
| ACAAACACAAAGCTTTGTGA+AGG | 0.602760 | 1:+76816986 | MsG0180004354.01.T01:CDS |
| ACCACATCCTAGGACACCAT+TGG | 0.613071 | 1:+76816598 | MsG0180004354.01.T01:CDS |
| AGTGTGTCAACTGAGTAATC+GGG | 0.617187 | 1:-76816830 | None:intergenic |
| GCATGTTGATGCAGTAGTGG+AGG | 0.620402 | 1:-76816665 | None:intergenic |
| TATCGACCACCAACTGACAT+CGG | 0.627712 | 1:-76817213 | None:intergenic |
| CAAACACAAAGCTTTGTGAA+GGG | 0.641318 | 1:+76816987 | MsG0180004354.01.T01:CDS |
| GCATGGATATTGAGATCCAA+CGG | 0.641639 | 1:-76817136 | None:intergenic |
| ATAGCATGTTGATGCAGTAG+TGG | 0.662876 | 1:-76816668 | None:intergenic |
| TCTTTACCTAAATTCTACCA+AGG | 0.672436 | 1:+76817010 | MsG0180004354.01.T01:CDS |
| ATTACTCAGTTGACACACTG+AGG | 0.689059 | 1:+76816834 | MsG0180004354.01.T01:CDS |
| TCTGATCTAGAGGAACCTGG+AGG | 0.738914 | 1:-76816623 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGGTATTTACTATGAGAA+AGG | + | Chr1:76816941-76816960 | MsG0180004354.01.T01:CDS | 25.0% |
| ! | TAAATACCAATCTTCTTGTT+TGG | - | Chr1:76816932-76816951 | None:intergenic | 25.0% |
| ! | TAATAATCTTGTAAGCATCA+AGG | + | Chr1:76817246-76817265 | MsG0180004354.01.T01:CDS | 25.0% |
| !! | AAATACCAATCTTCTTGTTT+GGG | - | Chr1:76816931-76816950 | None:intergenic | 25.0% |
| CACCAATTGCTATCAAATTT+GGG | + | Chr1:76817158-76817177 | MsG0180004354.01.T01:CDS | 30.0% | |
| GTATTTACTATGAGAAAGGA+GGG | + | Chr1:76816945-76816964 | MsG0180004354.01.T01:CDS | 30.0% | |
| TCTTTACCTAAATTCTACCA+AGG | + | Chr1:76817010-76817029 | MsG0180004354.01.T01:CDS | 30.0% | |
| ! | AATAACCTTTTCTGATCTAG+AGG | - | Chr1:76816636-76816655 | None:intergenic | 30.0% |
| ! | TTGAAGAAGGTGAAAGTTTT+GGG | + | Chr1:76817190-76817209 | MsG0180004354.01.T01:CDS | 30.0% |
| ! | TTGCAAAAACAGCTTCTTTT+TGG | - | Chr1:76816716-76816735 | None:intergenic | 30.0% |
| !!! | GTTTTGTTTCCACATTCATA+GGG | - | Chr1:76816580-76816599 | None:intergenic | 30.0% |
| GCACCAATTGCTATCAAATT+TGG | + | Chr1:76817157-76817176 | MsG0180004354.01.T01:CDS | 35.0% | |
| GGTATTTACTATGAGAAAGG+AGG | + | Chr1:76816944-76816963 | MsG0180004354.01.T01:CDS | 35.0% | |
| TGATGACCTTGGTAGAATTT+AGG | - | Chr1:76817019-76817038 | None:intergenic | 35.0% | |
| TGGTTTGAAGATGAGGTAAA+GGG | - | Chr1:76816804-76816823 | None:intergenic | 35.0% | |
| ! | ACAAACACAAAGCTTTGTGA+AGG | + | Chr1:76816986-76817005 | MsG0180004354.01.T01:CDS | 35.0% |
| ! | ATTTGATAGCAATTGGTGCA+TGG | - | Chr1:76817156-76817175 | None:intergenic | 35.0% |
| ! | CAAACACAAAGCTTTGTGAA+GGG | + | Chr1:76816987-76817006 | MsG0180004354.01.T01:CDS | 35.0% |
| ! | CAGCAATGATAATGAGAAGA+AGG | - | Chr1:76816769-76816788 | None:intergenic | 35.0% |
| ! | CTCCCAAATTTGATAGCAAT+TGG | - | Chr1:76817163-76817182 | None:intergenic | 35.0% |
| ! | GTGCTTAATGTGTCATTAAC+AGG | + | Chr1:76817049-76817068 | MsG0180004354.01.T01:CDS | 35.0% |
| ! | GTTGAAGAAGGTGAAAGTTT+TGG | + | Chr1:76817189-76817208 | MsG0180004354.01.T01:CDS | 35.0% |
| ! | GTTTTTGCAAGTGCATATGT+TGG | + | Chr1:76816726-76816745 | MsG0180004354.01.T01:CDS | 35.0% |
| ! | TTGGTTTGAAGATGAGGTAA+AGG | - | Chr1:76816805-76816824 | None:intergenic | 35.0% |
| !!! | GGTTTTGAAGAGTTTATTGC+CGG | - | Chr1:76816695-76816714 | None:intergenic | 35.0% |
| !!! | GGTTTTGTTTCCACATTCAT+AGG | - | Chr1:76816581-76816600 | None:intergenic | 35.0% |
| ACCAACCCAAACAAGAAGAT+TGG | + | Chr1:76816923-76816942 | MsG0180004354.01.T01:CDS | 40.0% | |
| AGGTTCCTCTAGATCAGAAA+AGG | + | Chr1:76816628-76816647 | MsG0180004354.01.T01:CDS | 40.0% | |
| AGTGTGTCAACTGAGTAATC+GGG | - | Chr1:76816833-76816852 | None:intergenic | 40.0% | |
| ATAGCATGTTGATGCAGTAG+TGG | - | Chr1:76816671-76816690 | None:intergenic | 40.0% | |
| ATTACTCAGTTGACACACTG+AGG | + | Chr1:76816834-76816853 | MsG0180004354.01.T01:CDS | 40.0% | |
| ATTATCATTGCTGCAACTGC+AGG | + | Chr1:76816776-76816795 | MsG0180004354.01.T01:CDS | 40.0% | |
| CAGTGTGTCAACTGAGTAAT+CGG | - | Chr1:76816834-76816853 | None:intergenic | 40.0% | |
| GCATGGATATTGAGATCCAA+CGG | - | Chr1:76817139-76817158 | None:intergenic | 40.0% | |
| GTCTGGAAACTCGTTAATGA+CGG | + | Chr1:76817081-76817100 | MsG0180004354.01.T01:CDS | 40.0% | |
| TGGAAACAAAACCACATCCT+AGG | + | Chr1:76816588-76816607 | MsG0180004354.01.T01:CDS | 40.0% | |
| TGGGAGATTGAAGTTGAAGA+AGG | + | Chr1:76817177-76817196 | MsG0180004354.01.T01:CDS | 40.0% | |
| TTAACAGGTCAAGTTCAGTC+TGG | + | Chr1:76817064-76817083 | MsG0180004354.01.T01:CDS | 40.0% | |
| TTGTTTCCACATTCATAGGG+TGG | - | Chr1:76816577-76816596 | None:intergenic | 40.0% | |
| ! | ACCAATCTTCTTGTTTGGGT+TGG | - | Chr1:76816927-76816946 | None:intergenic | 40.0% |
| ! | TTTTCTGATCTAGAGGAACC+TGG | - | Chr1:76816629-76816648 | None:intergenic | 40.0% |
| !! | CTGTTTTGTTCTGATGACCT+TGG | - | Chr1:76817030-76817049 | None:intergenic | 40.0% |
| !! | GTAGCTTTGGTTTGAAGATG+AGG | - | Chr1:76816811-76816830 | None:intergenic | 40.0% |
| ACTGAGTAATCGGGTAGCTT+TGG | - | Chr1:76816824-76816843 | None:intergenic | 45.0% | |
| ACTGCATCAACATGCTATGC+CGG | + | Chr1:76816673-76816692 | MsG0180004354.01.T01:CDS | 45.0% | |
| GAGAATCCACCCTATGAATG+TGG | + | Chr1:76816568-76816587 | MsG0180004354.01.T01:CDS | 45.0% | |
| GCAAACAGGAAGAATTCCGT+TGG | + | Chr1:76817120-76817139 | MsG0180004354.01.T01:CDS | 45.0% | |
| TATCGACCACCAACTGACAT+CGG | - | Chr1:76817216-76817235 | None:intergenic | 45.0% | |
| TTGCAGCAACAACAGCAAAC+AGG | + | Chr1:76817106-76817125 | MsG0180004354.01.T01:CDS | 45.0% | |
| ACCAATGGTGTCCTAGGATG+TGG | - | Chr1:76816602-76816621 | None:intergenic | 50.0% | |
| ACCACATCCTAGGACACCAT+TGG | + | Chr1:76816598-76816617 | MsG0180004354.01.T01:CDS | 50.0% | |
| GCATGTTGATGCAGTAGTGG+AGG | - | Chr1:76816668-76816687 | None:intergenic | 50.0% | |
| TCTGATCTAGAGGAACCTGG+AGG | - | Chr1:76816626-76816645 | None:intergenic | 50.0% | |
| ! | TGTTGATGCAGTAGTGGAGG+AGG | - | Chr1:76816665-76816684 | None:intergenic | 50.0% |
| !!! | AGTTTTGGGCCGATGTCAGT+TGG | + | Chr1:76817204-76817223 | MsG0180004354.01.T01:CDS | 50.0% |
| AGGACACCATTGGTACCTCC+AGG | + | Chr1:76816608-76816627 | MsG0180004354.01.T01:CDS | 55.0% | |
| TTTGGGCCGATGTCAGTTGG+TGG | + | Chr1:76817207-76817226 | MsG0180004354.01.T01:CDS | 55.0% | |
| !! | GAGGAACCTGGAGGTACCAA+TGG | - | Chr1:76816617-76816636 | None:intergenic | 55.0% |
| !! | GGAGGTACCAATGGTGTCCT+AGG | - | Chr1:76816608-76816627 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 76816548 | 76817300 | 76816548 | ID=MsG0180004354.01;Name=MsG0180004354.01 |
| Chr1 | mRNA | 76816548 | 76817300 | 76816548 | ID=MsG0180004354.01.T01;Parent=MsG0180004354.01;Name=MsG0180004354.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|250 |
| Chr1 | exon | 76816548 | 76817300 | 76816548 | ID=MsG0180004354.01.T01:exon:12664;Parent=MsG0180004354.01.T01 |
| Chr1 | CDS | 76816548 | 76817300 | 76816548 | ID=MsG0180004354.01.T01:cds;Parent=MsG0180004354.01.T01 |
| Gene Sequence |
| Protein sequence |
