AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004776.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004776.01.T01 MTR_1g086650 97.786 542 12 0 1 542 1 542 0.0 1098
MsG0180004776.01.T01 MTR_5g061520 52.254 488 222 8 54 535 5 487 2.47e-171 494
MsG0180004776.01.T01 MTR_5g091660 48.374 492 234 11 56 536 54 536 1.60e-151 445
MsG0180004776.01.T01 MTR_3g448430 47.158 475 231 8 64 529 48 511 1.13e-139 414
MsG0180004776.01.T01 MTR_4g415290 42.917 480 257 8 60 529 17 489 5.22e-124 373
MsG0180004776.01.T01 MTR_1g040500 41.408 483 265 8 64 534 20 496 8.79e-121 367
MsG0180004776.01.T01 MTR_7g067380 41.851 497 269 10 49 534 9 496 3.20e-120 363
MsG0180004776.01.T01 MTR_8g030620 42.050 478 258 8 64 529 27 497 1.11e-118 360
MsG0180004776.01.T01 MTR_5g080360 42.204 481 251 12 65 529 3 472 4.44e-117 355
MsG0180004776.01.T01 MTR_3g048330 54.255 282 119 4 257 533 1 277 2.29e-101 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004776.01.T01 AT1G02390 54.562 537 209 12 10 529 9 527 0.0 557
MsG0180004776.01.T01 AT4G01950 53.497 529 216 8 11 529 9 517 0.0 554
MsG0180004776.01.T01 AT4G01950 53.497 529 216 8 11 529 37 545 0.0 553
MsG0180004776.01.T01 AT1G06520 44.933 523 258 12 32 529 67 584 2.80e-137 410
MsG0180004776.01.T01 AT3G11430 40.771 493 261 10 55 529 2 481 2.52e-120 364
MsG0180004776.01.T01 AT2G38110 42.562 484 255 9 60 529 20 494 3.66e-119 361
MsG0180004776.01.T01 AT5G06090 41.718 489 260 13 56 529 1 479 4.04e-114 348
MsG0180004776.01.T01 AT1G01610 41.031 485 252 13 65 529 23 493 3.35e-110 338
MsG0180004776.01.T01 AT4G00400 40.675 504 274 11 48 536 6 499 3.88e-109 335
MsG0180004776.01.T01 AT3G11325 39.925 268 123 4 263 529 142 372 1.12e-58 200
MsG0180004776.01.T01 AT3G11325 36.190 105 62 2 74 173 9 113 6.00e-12 68.2
MsG0180004776.01.T01 AT3G11325 39.925 268 123 4 263 529 139 369 1.48e-58 200
MsG0180004776.01.T01 AT3G11325 36.190 105 62 2 74 173 6 110 6.13e-12 68.2

Find 99 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCGTGACCATTGGATTTA+TGG 0.144323 1:+82623683 None:intergenic
TCATGGAAAATAAGAGATTT+TGG 0.152354 1:+82623886 None:intergenic
AACATTGCTAATGACTTTAA+TGG 0.190558 1:+82623838 None:intergenic
ACATCTTCCAAGAAGAATTT+TGG 0.212114 1:+82624700 None:intergenic
TAGTTCTATGGAAGACTATT+AGG 0.234269 1:+82625037 None:intergenic
GTAGAGAACCTTATTTATTA+AGG 0.241243 1:-82623438 MsG0180004776.01.T01:CDS
AGTTCTGCCAAAATTCTTCT+TGG 0.295056 1:-82624707 MsG0180004776.01.T01:CDS
ACTTTAATGGAGTTGGTGTT+AGG 0.295074 1:+82623851 None:intergenic
ACTCCGTTATTAACATTTAG+TGG 0.297118 1:-82623739 MsG0180004776.01.T01:CDS
GGGCTAAACCTTAATAAATA+AGG 0.309796 1:+82623430 None:intergenic
TCTCTACAAGTGGTCCCTTC+TGG 0.310793 1:+82623454 None:intergenic
AATGCTACAAGCATGAAATA+TGG 0.313107 1:+82624874 None:intergenic
TGGTTACTATGTTGGGTTGA+TGG 0.314893 1:-82624536 MsG0180004776.01.T01:CDS
AAAGCAGAAAACTTTAGAAT+TGG 0.326140 1:-82624736 MsG0180004776.01.T01:CDS
TTTCCTCGCCGATCCTTTCC+CGG 0.335386 1:-82623299 MsG0180004776.01.T01:CDS
ACCGTGACCATTGGATTTAT+GGG 0.366623 1:+82623684 None:intergenic
GCGGAGATTGGTAACACTTT+GGG 0.373212 1:-82623175 MsG0180004776.01.T01:CDS
TACCAATCTCCGCTTGGATA+TGG 0.373360 1:+82623185 None:intergenic
TAGTAATTTACCTAGTGTTA+TGG 0.391849 1:-82624626 MsG0180004776.01.T01:CDS
AAGTGTTGCTAATAGTTCTA+TGG 0.400669 1:+82625025 None:intergenic
GTGGGTCTAATAAGGTTCTA+TGG 0.412472 1:+82623644 None:intergenic
TGCTGGTAGAGAGCTGAAAA+TGG 0.415660 1:-82624563 MsG0180004776.01.T01:CDS
TGGTTTGTGGTTACTATGTT+GGG 0.432444 1:-82624543 MsG0180004776.01.T01:CDS
CTTTAATGGAGTTGGTGTTA+GGG 0.440433 1:+82623852 None:intergenic
AAAGGCTCTTTAGATATGAT+TGG 0.442495 1:-82624469 MsG0180004776.01.T01:CDS
ATGGTTTGTGGTTACTATGT+TGG 0.443488 1:-82624544 MsG0180004776.01.T01:CDS
GAAATTGTGAAGGACGAACT+TGG 0.444462 1:+82623254 None:intergenic
AATGTAGAGTGGGTCTAATA+AGG 0.445586 1:+82623636 None:intergenic
TTGATGCTAGCTGGTAATGA+AGG 0.447543 1:-82623115 MsG0180004776.01.T01:CDS
TGCAAGTGTGATTCTAATAA+CGG 0.459105 1:+82623786 None:intergenic
AACCATATCCAAGCGGAGAT+TGG 0.459393 1:-82623187 MsG0180004776.01.T01:CDS
AGCGGAGATTGGTAACACTT+TGG 0.468303 1:-82623176 MsG0180004776.01.T01:CDS
GCTAATGACTTTAATGGAGT+TGG 0.480716 1:+82623844 None:intergenic
AAATTCTTCTTGGAAGATGT+TGG 0.491109 1:-82624697 MsG0180004776.01.T01:CDS
TGCTAGTGGTCTAAAGGCAT+TGG 0.493274 1:-82623332 MsG0180004776.01.T01:CDS
TGAGTTGCCTAAGGAGAAAA+CGG 0.493607 1:+82625064 None:intergenic
GCGAGAATGCTGAGGAATGT+AGG 0.496088 1:-82623222 MsG0180004776.01.T01:CDS
GATAAGTATTTGATGCTAGC+TGG 0.498355 1:-82623124 MsG0180004776.01.T01:CDS
ATGCTTGTAGCATTTGAAGC+TGG 0.505292 1:-82624865 MsG0180004776.01.T01:CDS
AGTAACTCAACCGTGTAAGC+CGG 0.512367 1:+82623280 None:intergenic
CACACATTTCAGAAAACAAA+GGG 0.516657 1:+82623410 None:intergenic
GTAACTCAACCGTGTAAGCC+GGG 0.517316 1:+82623281 None:intergenic
ACGTGTGTATCAACAGCAAC+TGG 0.520127 1:+82623376 None:intergenic
TTGAGTGTACTAAGCTTACA+AGG 0.526540 1:-82623150 MsG0180004776.01.T01:CDS
GATAATAGCACCAATTAAAA+CGG 0.528624 1:-82623554 MsG0180004776.01.T01:CDS
TACAACTGCTAGTGGTCTAA+AGG 0.529525 1:-82623338 MsG0180004776.01.T01:CDS
CGATCCTTTCCCGGCTTACA+CGG 0.534252 1:-82623290 MsG0180004776.01.T01:CDS
GATGTAGTTGTGTGTCCAGA+AGG 0.535316 1:-82623469 MsG0180004776.01.T01:CDS
TGATGCTAGCTGGTAATGAA+GGG 0.536177 1:-82623114 MsG0180004776.01.T01:CDS
CACAAGGTGACAAGAGAGGT+TGG 0.537297 1:-82623933 MsG0180004776.01.T01:CDS
AGTCATTAGCAATGTTAATG+TGG 0.538663 1:-82623831 MsG0180004776.01.T01:CDS
CGGGAATTGATGATGTGAGA+CGG 0.539136 1:+82623713 None:intergenic
TGGTCTAAAGGCATTGGACC+CGG 0.543424 1:-82623326 MsG0180004776.01.T01:CDS
CAATCAAGGTGGGAAGAAAG+TGG 0.547282 1:-82624653 MsG0180004776.01.T01:CDS
TAATTTACCTAGTGTTATGG+TGG 0.550460 1:-82624623 MsG0180004776.01.T01:CDS
AAACAAGTTCATGAAGGGAA+AGG 0.550915 1:-82624487 MsG0180004776.01.T01:CDS
GTAAAGATGACCGTGACCAT+TGG 0.557285 1:+82623675 None:intergenic
AGTCCACTAAATGTTAATAA+CGG 0.558530 1:+82623736 None:intergenic
ACGGAAAGGATTATTCCGTA+TGG 0.559562 1:+82623805 None:intergenic
CTTTGAAACAAGTTCATGAA+GGG 0.561165 1:-82624492 MsG0180004776.01.T01:CDS
ATGTTAATGTGGCTTCCATA+CGG 0.564900 1:-82623820 MsG0180004776.01.T01:CDS
AGAGAGCTGAAAATGGTTTG+TGG 0.566065 1:-82624556 MsG0180004776.01.T01:CDS
TCACACATTTCAGAAAACAA+AGG 0.572407 1:+82623409 None:intergenic
GCTTTGAAACAAGTTCATGA+AGG 0.575993 1:-82624493 MsG0180004776.01.T01:CDS
TAAATAAGGTTCTCTACAAG+TGG 0.581152 1:+82623444 None:intergenic
CGTGTAAGCCGGGAAAGGAT+CGG 0.581422 1:+82623291 None:intergenic
TTGAGGTGATCAATCAAGGT+GGG 0.582634 1:-82624663 MsG0180004776.01.T01:CDS
GAAATATGGAAACAAAGAAG+TGG 0.585989 1:+82624888 None:intergenic
AAATTGTGAAGGACGAACTT+GGG 0.587318 1:+82623255 None:intergenic
GCCCATAAATCCAATGGTCA+CGG 0.591886 1:-82623685 MsG0180004776.01.T01:CDS
GAAAATGCCCATAAATCCAA+TGG 0.599428 1:-82623691 MsG0180004776.01.T01:CDS
AATGAGAATGAAATGTAGAG+TGG 0.600880 1:+82623625 None:intergenic
TTAGGGCTAGTCTTCCATCA+TGG 0.606968 1:+82623869 None:intergenic
ATGAAAACGTTGTTACAACA+AGG 0.607933 1:-82623493 MsG0180004776.01.T01:CDS
AAACTCTCCACCATAACACT+AGG 0.610998 1:+82624616 None:intergenic
AAGTGTTACCAATCTCCGCT+TGG 0.611648 1:+82623179 None:intergenic
GAAAACGTTGTTACAACAAG+GGG 0.612525 1:-82623491 MsG0180004776.01.T01:CDS
TCAACCGTGTAAGCCGGGAA+AGG 0.614668 1:+82623286 None:intergenic
CTTGTAGCATTTGAAGCTGG+AGG 0.616751 1:-82624862 MsG0180004776.01.T01:CDS
GAAGTCTATGCTGTCTCACA+AGG 0.616778 1:-82623949 MsG0180004776.01.T01:CDS
ATTACACAAGAAATTAATTG+CGG 0.617882 1:-82623605 MsG0180004776.01.T01:CDS
TCAGCATTCTCGCTAACGCG+TGG 0.619138 1:+82623232 None:intergenic
GTGTGATTCTAATAACGGAA+AGG 0.623067 1:+82623791 None:intergenic
TGTGATCATTGAGGTCTGAT+CGG 0.623322 1:+82624947 None:intergenic
ACGCGTTAGCGAGAATGCTG+AGG 0.629609 1:-82623230 MsG0180004776.01.T01:CDS
ATGTAGTTGTGTGTCCAGAA+GGG 0.632491 1:-82623468 MsG0180004776.01.T01:CDS
AATCAAGGTGGGAAGAAAGT+GGG 0.634730 1:-82624652 MsG0180004776.01.T01:CDS
CTAACGCGTGGAAATTGTGA+AGG 0.640306 1:+82623244 None:intergenic
AGACTAACGTGTGATCATTG+AGG 0.644349 1:+82624938 None:intergenic
TGAAAACGTTGTTACAACAA+GGG 0.645168 1:-82623492 MsG0180004776.01.T01:CDS
ATGAGAATGAAATGTAGAGT+GGG 0.646114 1:+82623626 None:intergenic
GTCTCACAAGGTGACAAGAG+AGG 0.647294 1:-82623937 MsG0180004776.01.T01:CDS
GATGCTAGCTGGTAATGAAG+GGG 0.656969 1:-82623113 MsG0180004776.01.T01:CDS
TTTGAGGTGATCAATCAAGG+TGG 0.663961 1:-82624664 MsG0180004776.01.T01:CDS
GGTTATTGTTGACTGTTGCG+TGG 0.688629 1:+82623064 None:intergenic
TGAGGAATGTAGGTTTGAAG+TGG 0.690751 1:-82623212 MsG0180004776.01.T01:CDS
GTACAAAAGCACTAGCACAA+TGG 0.696547 1:+82624828 None:intergenic
AAGCCGGGAAAGGATCGGCG+AGG 0.747967 1:+82623296 None:intergenic
AGTGGCGAACCATATCCAAG+CGG 0.769808 1:-82623194 MsG0180004776.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTTAATTTATTTTCT+AGG - Chr1:82624073-82624092 MsG0180004776.01.T01:intron 10.0%
!!! TGTTGTATTTTTTTTTTTTA+AGG + Chr1:82624139-82624158 None:intergenic 10.0%
!!! AATTTCTTTTCTAGATATAT+TGG - Chr1:82624173-82624192 MsG0180004776.01.T01:intron 15.0%
!! ATTACACAAGAAATTAATTG+CGG - Chr1:82624578-82624597 MsG0180004776.01.T01:CDS 20.0%
!!! AAAAGATGAAAAGAGATTTT+AGG + Chr1:82623095-82623114 None:intergenic 20.0%
!!! AATTTATTTTCTAGGTATAC+TGG - Chr1:82624081-82624100 MsG0180004776.01.T01:intron 20.0%
!!! ATAAGTTGTTTTGTTATATC+AGG - Chr1:82624212-82624231 MsG0180004776.01.T01:intron 20.0%
!!! CAACTTATTAGTTTAATTCA+AGG + Chr1:82624200-82624219 None:intergenic 20.0%
!!! TAAAATCTCTTTTCATCTTT+TGG - Chr1:82623094-82623113 MsG0180004776.01.T01:CDS 20.0%
!!! TAAGAAGAGATGAAAATTTT+TGG + Chr1:82623215-82623234 None:intergenic 20.0%
!!! TTTTTATCAGGAGAAAAAAT+GGG - Chr1:82623387-82623406 MsG0180004776.01.T01:CDS 20.0%
! AAAAATGGGGTTGAATATAA+TGG - Chr1:82623401-82623420 MsG0180004776.01.T01:CDS 25.0%
! AACATTGCTAATGACTTTAA+TGG + Chr1:82624348-82624367 None:intergenic 25.0%
! AATTAATAGTTGGACACAAA+TGG + Chr1:82624020-82624039 None:intergenic 25.0%
! AGTCCACTAAATGTTAATAA+CGG + Chr1:82624450-82624469 None:intergenic 25.0%
! CTCCTGATAAAAAGATTAAA+GGG + Chr1:82623380-82623399 None:intergenic 25.0%
! GATAATAGCACCAATTAAAA+CGG - Chr1:82624629-82624648 MsG0180004776.01.T01:CDS 25.0%
! TAATTATTTGAGTCGTGTTA+AGG - Chr1:82623839-82623858 MsG0180004776.01.T01:CDS 25.0%
! TAGTAATTTACCTAGTGTTA+TGG - Chr1:82623557-82623576 MsG0180004776.01.T01:CDS 25.0%
! TCTCCTGATAAAAAGATTAA+AGG + Chr1:82623381-82623400 None:intergenic 25.0%
!! CATGGAAAATAAGAGATTTT+GGG + Chr1:82624299-82624318 None:intergenic 25.0%
!! CTTTTTATCAGGAGAAAAAA+TGG - Chr1:82623386-82623405 MsG0180004776.01.T01:CDS 25.0%
!! GTAGAGAACCTTATTTATTA+AGG - Chr1:82624745-82624764 MsG0180004776.01.T01:CDS 25.0%
!! TACCCTTTAATCTTTTTATC+AGG - Chr1:82623375-82623394 MsG0180004776.01.T01:CDS 25.0%
!! TCATGGAAAATAAGAGATTT+TGG + Chr1:82624300-82624319 None:intergenic 25.0%
!! TTTTATCAGGAGAAAAAATG+GGG - Chr1:82623388-82623407 MsG0180004776.01.T01:CDS 25.0%
!!! AAAGCAGAAAACTTTAGAAT+TGG - Chr1:82623447-82623466 MsG0180004776.01.T01:CDS 25.0%
!!! AAATCTCTTATTTTCCATGA+TGG - Chr1:82624300-82624319 MsG0180004776.01.T01:intron 25.0%
!!! TTGAAAATTGATTTTGTTGC+TGG - Chr1:82623603-82623622 MsG0180004776.01.T01:CDS 25.0%
AAAGGCTCTTTAGATATGAT+TGG - Chr1:82623714-82623733 MsG0180004776.01.T01:CDS 30.0%
AAATTCTTCTTGGAAGATGT+TGG - Chr1:82623486-82623505 MsG0180004776.01.T01:CDS 30.0%
AATGAGAATGAAATGTAGAG+TGG + Chr1:82624561-82624580 None:intergenic 30.0%
AATGCTACAAGCATGAAATA+TGG + Chr1:82623312-82623331 None:intergenic 30.0%
ACTCCGTTATTAACATTTAG+TGG - Chr1:82624444-82624463 MsG0180004776.01.T01:CDS 30.0%
AGTCATTAGCAATGTTAATG+TGG - Chr1:82624352-82624371 MsG0180004776.01.T01:intron 30.0%
ATGAAAACGTTGTTACAACA+AGG - Chr1:82624690-82624709 MsG0180004776.01.T01:CDS 30.0%
ATGAGAATGAAATGTAGAGT+GGG + Chr1:82624560-82624579 None:intergenic 30.0%
CACAAATGGTTAATGTACTA+TGG + Chr1:82624006-82624025 None:intergenic 30.0%
CACACATTTCAGAAAACAAA+GGG + Chr1:82624776-82624795 None:intergenic 30.0%
GAAATATGGAAACAAAGAAG+TGG + Chr1:82623298-82623317 None:intergenic 30.0%
GGGCTAAACCTTAATAAATA+AGG + Chr1:82624756-82624775 None:intergenic 30.0%
GGTTAAGGAAAAATGTTTGA+CGG + Chr1:82623985-82624004 None:intergenic 30.0%
TAAATAAGGTTCTCTACAAG+TGG + Chr1:82624742-82624761 None:intergenic 30.0%
TAATTTACCTAGTGTTATGG+TGG - Chr1:82623560-82623579 MsG0180004776.01.T01:CDS 30.0%
TCACACATTTCAGAAAACAA+AGG + Chr1:82624777-82624796 None:intergenic 30.0%
TGAAAACGTTGTTACAACAA+GGG - Chr1:82624691-82624710 MsG0180004776.01.T01:CDS 30.0%
TGCAAGTGTGATTCTAATAA+CGG + Chr1:82624400-82624419 None:intergenic 30.0%
TGGTTAATGTACTATGGTTA+AGG + Chr1:82624000-82624019 None:intergenic 30.0%
! AAGTGTTGCTAATAGTTCTA+TGG + Chr1:82623161-82623180 None:intergenic 30.0%
! ACATCTTCCAAGAAGAATTT+TGG + Chr1:82623486-82623505 None:intergenic 30.0%
! ATTGGATTTATGGGCATTTT+CGG + Chr1:82624493-82624512 None:intergenic 30.0%
! TAGTTCTATGGAAGACTATT+AGG + Chr1:82623149-82623168 None:intergenic 30.0%
! TGAAATTTTCTCTTCTTGCA+AGG - Chr1:82623767-82623786 MsG0180004776.01.T01:CDS 30.0%
!! CTTTGAAACAAGTTCATGAA+GGG - Chr1:82623691-82623710 MsG0180004776.01.T01:CDS 30.0%
!!! ATGGTCATGATATGCTTTTT+TGG - Chr1:82623420-82623439 MsG0180004776.01.T01:CDS 30.0%
!!! ATTTTTGAGGTGATCAATCA+AGG - Chr1:82623516-82623535 MsG0180004776.01.T01:CDS 30.0%
!!! TGTTGGTTCAGAAATTTTTG+AGG - Chr1:82623503-82623522 MsG0180004776.01.T01:CDS 30.0%
AAACAAGTTCATGAAGGGAA+AGG - Chr1:82623696-82623715 MsG0180004776.01.T01:CDS 35.0%
AAATTGTGAAGGACGAACTT+GGG + Chr1:82624931-82624950 None:intergenic 35.0%
AATGTAGAGTGGGTCTAATA+AGG + Chr1:82624550-82624569 None:intergenic 35.0%
AGTTCTGCCAAAATTCTTCT+TGG - Chr1:82623476-82623495 MsG0180004776.01.T01:CDS 35.0%
ATGTTAATGTGGCTTCCATA+CGG - Chr1:82624363-82624382 MsG0180004776.01.T01:intron 35.0%
GAAAACGTTGTTACAACAAG+GGG - Chr1:82624692-82624711 MsG0180004776.01.T01:CDS 35.0%
GAAAATGCCCATAAATCCAA+TGG - Chr1:82624492-82624511 MsG0180004776.01.T01:CDS 35.0%
GATAAGTATTTGATGCTAGC+TGG - Chr1:82625059-82625078 MsG0180004776.01.T01:CDS 35.0%
GCTAATGACTTTAATGGAGT+TGG + Chr1:82624342-82624361 None:intergenic 35.0%
TTGAGTGTACTAAGCTTACA+AGG - Chr1:82625033-82625052 MsG0180004776.01.T01:CDS 35.0%
! AACGGAGTTGAGATTTTGAA+TGG + Chr1:82624432-82624451 None:intergenic 35.0%
! ATGGTTTGTGGTTACTATGT+TGG - Chr1:82623639-82623658 MsG0180004776.01.T01:CDS 35.0%
! GTGTGATTCTAATAACGGAA+AGG + Chr1:82624395-82624414 None:intergenic 35.0%
! TGGTTTGTGGTTACTATGTT+GGG - Chr1:82623640-82623659 MsG0180004776.01.T01:CDS 35.0%
! TTGGATTTATGGGCATTTTC+GGG + Chr1:82624492-82624511 None:intergenic 35.0%
! TTTTATGGTACAACTGCTAG+TGG - Chr1:82624837-82624856 MsG0180004776.01.T01:CDS 35.0%
!! ACTTTAATGGAGTTGGTGTT+AGG + Chr1:82624335-82624354 None:intergenic 35.0%
!! CTTTAATGGAGTTGGTGTTA+GGG + Chr1:82624334-82624353 None:intergenic 35.0%
!! GCTTTGAAACAAGTTCATGA+AGG - Chr1:82623690-82623709 MsG0180004776.01.T01:CDS 35.0%
!!! TTAGGTTTTTGAGTTGCCTA+AGG + Chr1:82623131-82623150 None:intergenic 35.0%
AAACTCTCCACCATAACACT+AGG + Chr1:82623570-82623589 None:intergenic 40.0%
AATCAAGGTGGGAAGAAAGT+GGG - Chr1:82623531-82623550 MsG0180004776.01.T01:CDS 40.0%
ACCGTGACCATTGGATTTAT+GGG + Chr1:82624502-82624521 None:intergenic 40.0%
ACGGAAAGGATTATTCCGTA+TGG + Chr1:82624381-82624400 None:intergenic 40.0%
AGACTAACGTGTGATCATTG+AGG + Chr1:82623248-82623267 None:intergenic 40.0%
AGAGAGCTGAAAATGGTTTG+TGG - Chr1:82623627-82623646 MsG0180004776.01.T01:CDS 40.0%
ATGTAGTTGTGTGTCCAGAA+GGG - Chr1:82624715-82624734 MsG0180004776.01.T01:CDS 40.0%
GAAATTGTGAAGGACGAACT+TGG + Chr1:82624932-82624951 None:intergenic 40.0%
GTGGGTCTAATAAGGTTCTA+TGG + Chr1:82624542-82624561 None:intergenic 40.0%
TACAACTGCTAGTGGTCTAA+AGG - Chr1:82624845-82624864 MsG0180004776.01.T01:CDS 40.0%
TGAGGAATGTAGGTTTGAAG+TGG - Chr1:82624971-82624990 MsG0180004776.01.T01:CDS 40.0%
TGAGTTGCCTAAGGAGAAAA+CGG + Chr1:82623122-82623141 None:intergenic 40.0%
TGTGATCATTGAGGTCTGAT+CGG + Chr1:82623239-82623258 None:intergenic 40.0%
TTGAGGTGATCAATCAAGGT+GGG - Chr1:82623520-82623539 MsG0180004776.01.T01:CDS 40.0%
! AAGGGGTAGTTTTCAACAGA+AGG - Chr1:82625087-82625106 MsG0180004776.01.T01:CDS 40.0%
! ACACACGTCAGCATGTTTTA+TGG - Chr1:82624822-82624841 MsG0180004776.01.T01:CDS 40.0%
! ATGCTTGTAGCATTTGAAGC+TGG - Chr1:82623318-82623337 MsG0180004776.01.T01:CDS 40.0%
! TGATGCTAGCTGGTAATGAA+GGG - Chr1:82625069-82625088 MsG0180004776.01.T01:CDS 40.0%
! TTGATGCTAGCTGGTAATGA+AGG - Chr1:82625068-82625087 MsG0180004776.01.T01:CDS 40.0%
! TTTGAGGTGATCAATCAAGG+TGG - Chr1:82623519-82623538 MsG0180004776.01.T01:CDS 40.0%
! TTTGGTACCGTTTTCTCCTT+AGG - Chr1:82623112-82623131 MsG0180004776.01.T01:CDS 40.0%
!! GACAAACGCACCGTTTTAAT+TGG + Chr1:82624642-82624661 None:intergenic 40.0%
!! GTACAAAAGCACTAGCACAA+TGG + Chr1:82623358-82623377 None:intergenic 40.0%
!! TGGTTACTATGTTGGGTTGA+TGG - Chr1:82623647-82623666 MsG0180004776.01.T01:CDS 40.0%
AACCATATCCAAGCGGAGAT+TGG - Chr1:82624996-82625015 MsG0180004776.01.T01:CDS 45.0%
AAGTGTTACCAATCTCCGCT+TGG + Chr1:82625007-82625026 None:intergenic 45.0%
ACGTGTGTATCAACAGCAAC+TGG + Chr1:82624810-82624829 None:intergenic 45.0%
AGTAACTCAACCGTGTAAGC+CGG + Chr1:82624906-82624925 None:intergenic 45.0%
CAATCAAGGTGGGAAGAAAG+TGG - Chr1:82623530-82623549 MsG0180004776.01.T01:CDS 45.0%
CTAACGCGTGGAAATTGTGA+AGG + Chr1:82624942-82624961 None:intergenic 45.0%
GAAGTCTATGCTGTCTCACA+AGG - Chr1:82624234-82624253 MsG0180004776.01.T01:intron 45.0%
GACCGTGACCATTGGATTTA+TGG + Chr1:82624503-82624522 None:intergenic 45.0%
GATGTAGTTGTGTGTCCAGA+AGG - Chr1:82624714-82624733 MsG0180004776.01.T01:CDS 45.0%
GCCCATAAATCCAATGGTCA+CGG - Chr1:82624498-82624517 MsG0180004776.01.T01:CDS 45.0%
GTAAAGATGACCGTGACCAT+TGG + Chr1:82624511-82624530 None:intergenic 45.0%
TACCAATCTCCGCTTGGATA+TGG + Chr1:82625001-82625020 None:intergenic 45.0%
TGCTGGTAGAGAGCTGAAAA+TGG - Chr1:82623620-82623639 MsG0180004776.01.T01:CDS 45.0%
! AGCGGAGATTGGTAACACTT+TGG - Chr1:82625007-82625026 MsG0180004776.01.T01:CDS 45.0%
! CTTGTAGCATTTGAAGCTGG+AGG - Chr1:82623321-82623340 MsG0180004776.01.T01:CDS 45.0%
! GATGCTAGCTGGTAATGAAG+GGG - Chr1:82625070-82625089 MsG0180004776.01.T01:CDS 45.0%
! GCGGAGATTGGTAACACTTT+GGG - Chr1:82625008-82625027 MsG0180004776.01.T01:CDS 45.0%
! TGCTAGTGGTCTAAAGGCAT+TGG - Chr1:82624851-82624870 MsG0180004776.01.T01:CDS 45.0%
!! CGGGAATTGATGATGTGAGA+CGG + Chr1:82624473-82624492 None:intergenic 45.0%
!! TTAGGGCTAGTCTTCCATCA+TGG + Chr1:82624317-82624336 None:intergenic 45.0%
!! ATAAAAATTAAATTAATAGT+TGG + Chr1:82624030-82624049 None:intergenic 5.0%
!! ATTAAAATAAAATAATTGAT+TGG + Chr1:82623823-82623842 None:intergenic 5.0%
AGTGGCGAACCATATCCAAG+CGG - Chr1:82624989-82625008 MsG0180004776.01.T01:CDS 50.0%
CACAAGGTGACAAGAGAGGT+TGG - Chr1:82624250-82624269 MsG0180004776.01.T01:intron 50.0%
GATCGGCGAGGAAAAAAACC+GGG + Chr1:82624878-82624897 None:intergenic 50.0%
GCGAGAATGCTGAGGAATGT+AGG - Chr1:82624961-82624980 MsG0180004776.01.T01:CDS 50.0%
GGATCGGCGAGGAAAAAAAC+CGG + Chr1:82624879-82624898 None:intergenic 50.0%
GTAACTCAACCGTGTAAGCC+GGG + Chr1:82624905-82624924 None:intergenic 50.0%
GTCTCACAAGGTGACAAGAG+AGG - Chr1:82624246-82624265 MsG0180004776.01.T01:intron 50.0%
TCTCTACAAGTGGTCCCTTC+TGG + Chr1:82624732-82624751 None:intergenic 50.0%
!! TGGTCTAAAGGCATTGGACC+CGG - Chr1:82624857-82624876 MsG0180004776.01.T01:CDS 50.0%
ACGCGTTAGCGAGAATGCTG+AGG - Chr1:82624953-82624972 MsG0180004776.01.T01:CDS 55.0%
CGATCCTTTCCCGGCTTACA+CGG - Chr1:82624893-82624912 MsG0180004776.01.T01:CDS 55.0%
CGTGTAAGCCGGGAAAGGAT+CGG + Chr1:82624895-82624914 None:intergenic 55.0%
TCAACCGTGTAAGCCGGGAA+AGG + Chr1:82624900-82624919 None:intergenic 55.0%
TCAGCATTCTCGCTAACGCG+TGG + Chr1:82624954-82624973 None:intergenic 55.0%
TTTCCTCGCCGATCCTTTCC+CGG - Chr1:82624884-82624903 MsG0180004776.01.T01:CDS 55.0%
AAGCCGGGAAAGGATCGGCG+AGG + Chr1:82624890-82624909 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82623066 82625139 82623066 ID=MsG0180004776.01;Name=MsG0180004776.01
Chr1 mRNA 82623066 82625139 82623066 ID=MsG0180004776.01.T01;Parent=MsG0180004776.01;Name=MsG0180004776.01.T01;_AED=0.17;_eAED=0.18;_QI=0|0|0|1|1|1|2|0|542
Chr1 exon 82624417 82625139 82624417 ID=MsG0180004776.01.T01:exon:8601;Parent=MsG0180004776.01.T01
Chr1 exon 82623066 82623971 82623066 ID=MsG0180004776.01.T01:exon:8600;Parent=MsG0180004776.01.T01
Chr1 CDS 82624417 82625139 82624417 ID=MsG0180004776.01.T01:cds;Parent=MsG0180004776.01.T01
Chr1 CDS 82623066 82623971 82623066 ID=MsG0180004776.01.T01:cds;Parent=MsG0180004776.01.T01
Gene Sequence

>MsG0180004776.01.T01

ATGGCAAAATTGTTTAGAGCATATTTCCTAAAATCTCTTTTCATCTTTTGGTACCGTTTTCTCCTTAGGCAACTCAAAAACCTAATAGTCTTCCATAGAACTATTAGCAACACTTTTGCTAATACAACACAATCACATATCAAATACCAAAAATTTTCATCTCTTCTTAACCGATCAGACCTCAATGATCACACGTTAGTCTTCGACGTCGAAAACACTTTACTTAAATCCACTTCTTTGTTTCCATATTTCATGCTTGTAGCATTTGAAGCTGGAGGACTACTTAGAGCCATTGTGCTAGTGCTTTTGTACCCTTTAATCTTTTTATCAGGAGAAAAAATGGGGTTGAATATAATGGTCATGATATGCTTTTTTGGTCTCAAAGCAGAAAACTTTAGAATTGGAAGATCAGTTCTGCCAAAATTCTTCTTGGAAGATGTTGGTTCAGAAATTTTTGAGGTGATCAATCAAGGTGGGAAGAAAGTGGGTGTTAGTAATTTACCTAGTGTTATGGTGGAGAGTTTCTTGAAAGAGTATTTGAAAATTGATTTTGTTGCTGGTAGAGAGCTGAAAATGGTTTGTGGTTACTATGTTGGGTTGATGGATGAGACAAAAAATATGCATGCTTTGAAACAAGTTCATGAAGGGAAAGGCTCTTTAGATATGATTGGTATTACTAGATTCAATAACATTCATGATGATGAAATTTTCTCTTCTTGCAAGGAAGTCTATGCTGTCTCACAAGGTGACAAGAGAGGTTGGAAAAAGCTAGCAACAAAAAAATACCCAAAATCTCTTATTTTCCATGATGGAAGACTAGCCCTAACACCAACTCCATTAAAGTCATTAGCAATGTTAATGTGGCTTCCATACGGAATAATCCTTTCCGTTATTAGAATCACACTTGCATTCACACTTCCATTCAAAATCTCAACTCCGTTATTAACATTTAGTGGACTCCGTCTCACATCATCAATTCCCGAAAATGCCCATAAATCCAATGGTCACGGTCATCTTTACGTGTGTAACCATAGAACCTTATTAGACCCACTCTACATTTCATTCTCATTACACAAGAAATTAATTGCGGTAACTTATAGTTTAAGTAAAATGTCTGAGATAATAGCACCAATTAAAACGGTGCGTTTGTCAAGAAATAGAGATATTGATGCAAAAATGATGAAAACGTTGTTACAACAAGGGGATGTAGTTGTGTGTCCAGAAGGGACCACTTGTAGAGAACCTTATTTATTAAGGTTTAGCCCTTTGTTTTCTGAAATGTGTGATGAGATAACACCAGTTGCTGTTGATACACACGTCAGCATGTTTTATGGTACAACTGCTAGTGGTCTAAAGGCATTGGACCCGGTTTTTTTCCTCGCCGATCCTTTCCCGGCTTACACGGTTGAGTTACTGACCCAAGTTCGTCCTTCACAATTTCCACGCGTTAGCGAGAATGCTGAGGAATGTAGGTTTGAAGTGGCGAACCATATCCAAGCGGAGATTGGTAACACTTTGGGATTTGAGTGTACTAAGCTTACAAGGAAAGATAAGTATTTGATGCTAGCTGGTAATGAAGGGGTAGTTTTCAACAGAAGGAACAGCAAACCACGCAACAGTCAACAATAA
Protein sequence

>MsG0180004776.01.T01

MAKLFRAYFLKSLFIFWYRFLLRQLKNLIVFHRTISNTFANTTQSHIKYQKFSSLLNRSDLNDHTLVFDVENTLLKSTSLFPYFMLVAFEAGGLLRAIVLVLLYPLIFLSGEKMGLNIMVMICFFGLKAENFRIGRSVLPKFFLEDVGSEIFEVINQGGKKVGVSNLPSVMVESFLKEYLKIDFVAGRELKMVCGYYVGLMDETKNMHALKQVHEGKGSLDMIGITRFNNIHDDEIFSSCKEVYAVSQGDKRGWKKLATKKYPKSLIFHDGRLALTPTPLKSLAMLMWLPYGIILSVIRITLAFTLPFKISTPLLTFSGLRLTSSIPENAHKSNGHGHLYVCNHRTLLDPLYISFSLHKKLIAVTYSLSKMSEIIAPIKTVRLSRNRDIDAKMMKTLLQQGDVVVCPEGTTCREPYLLRFSPLFSEMCDEITPVAVDTHVSMFYGTTASGLKALDPVFFLADPFPAYTVELLTQVRPSQFPRVSENAEECRFEVANHIQAEIGNTLGFECTKLTRKDKYLMLAGNEGVVFNRRNSKPRNSQQ*