AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004884.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004884.01.T01 MTR_1g088870 97.318 261 7 0 1 261 1 261 0.0 521
MsG0180004884.01.T01 MTR_1g088870 97.318 261 7 0 1 261 1 261 0.0 519
MsG0180004884.01.T01 MTR_1g088870 94.815 270 14 0 1 270 1 270 0.0 519
MsG0180004884.01.T01 MTR_1g088870 94.815 270 14 0 1 270 1 270 0.0 518
MsG0180004884.01.T01 MTR_1g088870 97.541 122 3 0 140 261 78 199 5.76e-80 244
MsG0180004884.01.T01 MTR_1g088870 97.541 122 3 0 140 261 78 199 6.53e-79 245
MsG0180004884.01.T01 MTR_1g088870 92.366 131 10 0 140 270 78 208 7.12e-79 244
MsG0180004884.01.T01 MTR_1g088870 91.379 116 10 0 155 270 1 116 7.44e-68 213
MsG0180004884.01.T01 MTR_1g088870 91.379 116 10 0 155 270 1 116 9.22e-68 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004884.01.T01 AT2G47830 59.846 259 90 3 3 261 4 248 1.40e-99 299
MsG0180004884.01.T01 AT2G47830 70.492 122 25 1 140 261 6 116 3.04e-52 174

Find 84 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATCTCTTGTATGCTTT+TGG 0.220512 1:-83962376 MsG0180004884.01.T01:CDS
CTCCTTCTTTAAGATGCTTT+GGG 0.231408 1:+83963512 None:intergenic
GCATTTACCGGTTACCTTTC+CGG 0.240823 1:-83963436 MsG0180004884.01.T01:CDS
ATTGCTTGGTTAACCATTTC+AGG 0.242161 1:+83962165 None:intergenic
TCTAAACATGCAGACTTTAC+TGG 0.260186 1:-83961591 MsG0180004884.01.T01:intron
TGTGATGCATGTTCGGTAAA+TGG 0.269458 1:+83963687 None:intergenic
TCTCCTTCTTTAAGATGCTT+TGG 0.277411 1:+83963511 None:intergenic
TCATCAGTTGTTGCTCTTGT+TGG 0.291502 1:-83961490 MsG0180004884.01.T01:CDS
CATGGTAAATTTGAGACTTT+AGG 0.302901 1:-83962405 MsG0180004884.01.T01:CDS
TCTTCTTCAAATTGTTGTCT+AGG 0.317446 1:+83963759 None:intergenic
TATGGGTGTTCTTTATCTCT+TGG 0.322357 1:+83963229 None:intergenic
ACCACATACCACATCGGATA+TGG 0.328439 1:+83963381 None:intergenic
TTTGAGACTTTAGGAGCTCT+TGG 0.365533 1:-83962396 MsG0180004884.01.T01:CDS
ATTCCTCACCGATGGCATTT+CGG 0.380815 1:-83963565 MsG0180004884.01.T01:CDS
TCCGGTGGCTAAAGCAATAT+CGG 0.383563 1:+83963462 None:intergenic
TTCCTCAGCTGGCCCTGAAA+TGG 0.406549 1:-83962178 MsG0180004884.01.T01:CDS
GTTCGCCATGCTCGTGTAAA+GGG 0.411295 1:+83963870 None:intergenic
CTTCTTCAAATTGTTGTCTA+GGG 0.412765 1:+83963760 None:intergenic
TGAAAGAAACACGGTGGCTC+CGG 0.440011 1:+83963631 None:intergenic
AATATGACTCTTGTGTCAAT+AGG 0.441249 1:-83962069 MsG0180004884.01.T01:CDS
TGGCGAACAACATCGCGTTC+AGG 0.444247 1:-83963855 MsG0180004884.01.T01:five_prime_UTR
TTTGTGTTGCAGGTTCTTAG+TGG 0.446788 1:-83963286 MsG0180004884.01.T01:intron
TGAATATTTGAAAGGTCTCT+AGG 0.451641 1:-83961452 None:intergenic
AATTGTTGTCTAGGGTTTCG+TGG 0.464904 1:+83963768 None:intergenic
GCCACCGGAAAAGCATTTAC+CGG 0.466119 1:-83963448 MsG0180004884.01.T01:CDS
TGCTCTTGTTGGAGTTGGTA+AGG 0.469374 1:-83961479 MsG0180004884.01.T01:CDS
TATGCACATGTGCCATTGCT+TGG 0.470012 1:+83962151 None:intergenic
GGTACTACCGGAAAGGTAAC+CGG 0.477619 1:+83963429 None:intergenic
TTGCTTGGTTAACCATTTCA+GGG 0.481120 1:+83962166 None:intergenic
GTTGTTGCTCTTGTTGGAGT+TGG 0.485769 1:-83961484 MsG0180004884.01.T01:CDS
GTGATGCATGTTCGGTAAAT+GGG 0.489974 1:+83963688 None:intergenic
GGCGATAATCGCGGTACTAC+CGG 0.490686 1:+83963417 None:intergenic
AAACACGGTGGCTCCGGCGG+TGG 0.491559 1:+83963637 None:intergenic
GTGTCAATAGGTGTTAAAGA+AGG 0.492325 1:-83962057 MsG0180004884.01.T01:CDS
GTGGCTCCGGCGGTGGAAGG+AGG 0.494924 1:+83963644 None:intergenic
AAGGAATTTGAATATTTGAA+AGG 0.503984 1:-83961460 MsG0180004884.01.T01:CDS
ACGGTGGCTCCGGCGGTGGA+AGG 0.504274 1:+83963641 None:intergenic
GCCGATATTGCTTTAGCCAC+CGG 0.506335 1:-83963463 MsG0180004884.01.T01:CDS
ATTGTTGTCTAGGGTTTCGT+GGG 0.506884 1:+83963769 None:intergenic
TGTTCGCCATGCTCGTGTAA+AGG 0.510506 1:+83963869 None:intergenic
GTCATATTCAAGGCAAGTAT+AGG 0.512743 1:+83962084 None:intergenic
AAAGGCGTGTGATGCATGTT+CGG 0.518647 1:+83963680 None:intergenic
AGAGATAAAGAACACCCATA+TGG 0.523745 1:-83963226 MsG0180004884.01.T01:intron
TCCTTCTTTAAGATGCTTTG+GGG 0.524357 1:+83963513 None:intergenic
AGAAGAAGAAGAAAGACGAT+GGG 0.531464 1:-83963733 MsG0180004884.01.T01:exon
AAGAAGAAGAAGAAAGACGA+TGG 0.532687 1:-83963734 MsG0180004884.01.T01:exon
TGACACAAGAGTCATATTCA+AGG 0.536100 1:+83962074 None:intergenic
ATGCCATGCAATGCCACCCC+CGG 0.537939 1:+83962350 None:intergenic
TACCACATCGGATATGGAAT+GGG 0.538057 1:+83963387 None:intergenic
CCTTCTTTAAGATGCTTTGG+GGG 0.555257 1:+83963514 None:intergenic
ATACCACATCGGATATGGAA+TGG 0.558450 1:+83963386 None:intergenic
GGATATGGAATGGGCAGCAT+CGG 0.560230 1:+83963396 None:intergenic
GATGATCCATGTCAATTCCA+TGG 0.564132 1:+83962106 None:intergenic
AAAACACCTCCTTCCACCGC+CGG 0.565480 1:-83963650 MsG0180004884.01.T01:CDS
GCTGGTGAAAGGCAAGGCAG+TGG 0.568004 1:-83961556 MsG0180004884.01.T01:CDS
TAACAAAACAAGCTGGTGAA+AGG 0.568414 1:-83961567 MsG0180004884.01.T01:CDS
TACTGGATAACAAAACAAGC+TGG 0.568456 1:-83961574 MsG0180004884.01.T01:CDS
TGTCAATAGGTGTTAAAGAA+GGG 0.570689 1:-83962056 MsG0180004884.01.T01:intron
TACAAATCAAGTGACCATAT+GGG 0.584409 1:+83963212 None:intergenic
AAGAAACACGGTGGCTCCGG+CGG 0.594321 1:+83963634 None:intergenic
AACCATTTCAGGGCCAGCTG+AGG 0.606652 1:+83962176 None:intergenic
CATTCAAAATTCCTCACCGA+TGG 0.609863 1:-83963573 MsG0180004884.01.T01:CDS
CTGGTGAAAGGCAAGGCAGT+GGG 0.614160 1:-83961555 MsG0180004884.01.T01:CDS
CAGTTCCCTTTACACGAGCA+TGG 0.616624 1:-83963875 MsG0180004884.01.T01:five_prime_UTR
ACACCACCATGGAATTGACA+TGG 0.617331 1:-83962112 MsG0180004884.01.T01:CDS
GGTGGTGTCCGCCTTGTCCG+TGG 0.617548 1:+83962127 None:intergenic
TGAAATGGTTAACCAAGCAA+TGG 0.629533 1:-83962163 MsG0180004884.01.T01:CDS
CATGTGCATAGCCACGGACA+AGG 0.633861 1:-83962138 MsG0180004884.01.T01:CDS
GGACAAGGCGGACACCACCA+TGG 0.635925 1:-83962123 MsG0180004884.01.T01:CDS
GAGAATGACCGAAATGCCAT+CGG 0.639151 1:+83963557 None:intergenic
TAATCGCGGTACTACCGGAA+AGG 0.640449 1:+83963422 None:intergenic
GGCAGCATCGGCGATAATCG+CGG 0.648431 1:+83963408 None:intergenic
ATGGCACATGTGCATAGCCA+CGG 0.652142 1:-83962144 MsG0180004884.01.T01:CDS
AAACAAGCTGGTGAAAGGCA+AGG 0.652822 1:-83961562 MsG0180004884.01.T01:CDS
TGCCCATTCCATATCCGATG+TGG 0.668505 1:-83963389 MsG0180004884.01.T01:intron
TGTGTTACCACATACCACAT+CGG 0.680747 1:+83963375 None:intergenic
GGTTGATGAAAGCAAATGCA+TGG 0.683265 1:-83961534 MsG0180004884.01.T01:CDS
TGACCGAAATGCCATCGGTG+AGG 0.689847 1:+83963562 None:intergenic
GTGCATAGCCACGGACAAGG+CGG 0.713830 1:-83962135 MsG0180004884.01.T01:CDS
GCTTCTGATTGAAAGAAACA+CGG 0.722013 1:+83963622 None:intergenic
GGTGGCTAAAGCAATATCGG+CGG 0.730480 1:+83963465 None:intergenic
GATCCATGTCAATTCCATGG+TGG 0.752390 1:+83962109 None:intergenic
TCTGATTGAAAGAAACACGG+TGG 0.779599 1:+83963625 None:intergenic
CTCGTGTAAAGGGAACTGTG+TGG 0.816803 1:+83963880 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAATTTCATTTTTCAT+GGG + Chr1:83963100-83963119 None:intergenic 10.0%
!!! TTTAAAAATTTCATTTTTCA+TGG + Chr1:83963101-83963120 None:intergenic 10.0%
!! AAAATAAAGTCAAATTGTAT+AGG - Chr1:83962361-83962380 MsG0180004884.01.T01:CDS 15.0%
!! ACATTATTATAATTTATGCT+AGG + Chr1:83962627-83962646 None:intergenic 15.0%
!! AAATATAAATATTGATGTCC+TGG + Chr1:83963748-83963767 None:intergenic 20.0%
!! AAGGAATTTGAATATTTGAA+AGG - Chr1:83963903-83963922 MsG0180004884.01.T01:five_prime_UTR 20.0%
!! AATTATACTAAAGCGTAAAA+CGG + Chr1:83962844-83962863 None:intergenic 20.0%
!! CAATAATAAAACACAAAATC+TGG + Chr1:83963717-83963736 None:intergenic 20.0%
!! GTTTGGATAAACAATTTAAT+TGG - Chr1:83962293-83962312 MsG0180004884.01.T01:intron 20.0%
!!! TAGTCTTCAGATTTTTAATT+TGG + Chr1:83963531-83963550 None:intergenic 20.0%
!!! TTATTGATTGTAGTTTTTAG+TGG - Chr1:83962033-83962052 MsG0180004884.01.T01:intron 20.0%
! AAGTAGACTATAGATACAAA+AGG - Chr1:83963498-83963517 MsG0180004884.01.T01:CDS 25.0%
! AATAAATGTCAGAGAATACT+AGG + Chr1:83963376-83963395 None:intergenic 25.0%
! ACATAAACAAATGTTCTGTT+TGG + Chr1:83962676-83962695 None:intergenic 25.0%
! CTTATTACAATACTCAGTAA+TGG + Chr1:83963338-83963357 None:intergenic 25.0%
! CTTATTTCCATAAAATCTCT+AGG + Chr1:83962412-83962431 None:intergenic 25.0%
! TATTTGACAAATCACATGAA+TGG + Chr1:83962871-83962890 None:intergenic 25.0%
!! ATACTATCCTAGAGATTTTA+TGG - Chr1:83962402-83962421 MsG0180004884.01.T01:CDS 25.0%
!! TAGGAGTTTAAGTTTATGTT+TGG - Chr1:83962276-83962295 MsG0180004884.01.T01:intron 25.0%
!! TTCATGTGATTTGTCAAATA+CGG - Chr1:83962871-83962890 MsG0180004884.01.T01:intron 25.0%
!! TTTATTATTGTTTCTTCTCC+AGG - Chr1:83963727-83963746 MsG0180004884.01.T01:exon 25.0%
!!! AATTTCATTTTTCATGGGAA+CGG + Chr1:83963095-83963114 None:intergenic 25.0%
AATATGACTCTTGTGTCAAT+AGG - Chr1:83963294-83963313 MsG0180004884.01.T01:intron 30.0%
ACGGCTTAGTAAAAAAATTC+AGG + Chr1:83963582-83963601 None:intergenic 30.0%
ATACAAATCAAGTGACCATA+TGG + Chr1:83962155-83962174 None:intergenic 30.0%
CGGCTTAGTAAAAAAATTCA+GGG + Chr1:83963581-83963600 None:intergenic 30.0%
CTTCTTCAAATTGTTGTCTA+GGG + Chr1:83961606-83961625 None:intergenic 30.0%
TAAAGCGTAAAACGGTAAAA+GGG + Chr1:83962836-83962855 None:intergenic 30.0%
TACAAATCAAGTGACCATAT+GGG + Chr1:83962154-83962173 None:intergenic 30.0%
TCTTCTTCAAATTGTTGTCT+AGG + Chr1:83961607-83961626 None:intergenic 30.0%
TGTCAATAGGTGTTAAAGAA+GGG - Chr1:83963307-83963326 MsG0180004884.01.T01:intron 30.0%
TTTCTCTTTCATTGTATCTC+AGG - Chr1:83962934-83962953 MsG0180004884.01.T01:intron 30.0%
TTTGTATCTTATGCTCAAGT+TGG + Chr1:83963471-83963490 None:intergenic 30.0%
! AATTTTGAATGCTGGGTTTT+GGG + Chr1:83961781-83961800 None:intergenic 30.0%
! ACTGAGTATTGTAATAAGAC+AGG - Chr1:83963340-83963359 MsG0180004884.01.T01:intron 30.0%
! AGTTTTACTGAACTCTAACT+AGG - Chr1:83963429-83963448 MsG0180004884.01.T01:CDS 30.0%
! CATGGTAAATTTGAGACTTT+AGG - Chr1:83962958-83962977 MsG0180004884.01.T01:intron 30.0%
! CTGAGTATTGTAATAAGACA+GGG - Chr1:83963341-83963360 MsG0180004884.01.T01:intron 30.0%
!! AAATTGTTTCTATAAGCTCG+CGG - Chr1:83962457-83962476 MsG0180004884.01.T01:intron 30.0%
!! ATTTCATTTTTCATGGGAAC+GGG + Chr1:83963094-83963113 None:intergenic 30.0%
!!! GTTGTCTTTTAAAGTTGCAA+AGG - Chr1:83962109-83962128 MsG0180004884.01.T01:CDS 30.0%
AAAAAGACAGGATGAAGGAA+TGG + Chr1:83963677-83963696 None:intergenic 35.0%
AAATCTGGTATTCAGCATAG+AGG + Chr1:83963702-83963721 None:intergenic 35.0%
AAGAAGAAGAAGAAAGACGA+TGG - Chr1:83961629-83961648 MsG0180004884.01.T01:intron 35.0%
AATGCTGATATACACATGCA+GGG - Chr1:83963159-83963178 MsG0180004884.01.T01:intron 35.0%
AGAAGAAGAAGAAAGACGAT+GGG - Chr1:83961630-83961649 MsG0180004884.01.T01:intron 35.0%
AGAGATAAAGAACACCCATA+TGG - Chr1:83962137-83962156 MsG0180004884.01.T01:CDS 35.0%
AGCAGTAACAACTTATGAGT+AGG - Chr1:83962430-83962449 MsG0180004884.01.T01:intron 35.0%
ATTGCTTGGTTAACCATTTC+AGG + Chr1:83963201-83963220 None:intergenic 35.0%
CATGCATAGAATAGTAGCAT+AGG + Chr1:83963406-83963425 None:intergenic 35.0%
CTAAAGCGTAAAACGGTAAA+AGG + Chr1:83962837-83962856 None:intergenic 35.0%
GCAGTAACAACTTATGAGTA+GGG - Chr1:83962431-83962450 MsG0180004884.01.T01:intron 35.0%
GCTTCTGATTGAAAGAAACA+CGG + Chr1:83961744-83961763 None:intergenic 35.0%
GGCTAACATAACTACAAACA+CGG + Chr1:83963601-83963620 None:intergenic 35.0%
GTCATATTCAAGGCAAGTAT+AGG + Chr1:83963282-83963301 None:intergenic 35.0%
GTGTCAATAGGTGTTAAAGA+AGG - Chr1:83963306-83963325 MsG0180004884.01.T01:intron 35.0%
TAACAAAACAAGCTGGTGAA+AGG - Chr1:83963796-83963815 MsG0180004884.01.T01:five_prime_UTR 35.0%
TAATGATCTTTGTCACGTGA+CGG - Chr1:83962697-83962716 MsG0180004884.01.T01:intron 35.0%
TAATGCTGATATACACATGC+AGG - Chr1:83963158-83963177 MsG0180004884.01.T01:intron 35.0%
TACTGGATAACAAAACAAGC+TGG - Chr1:83963789-83963808 MsG0180004884.01.T01:five_prime_UTR 35.0%
TATGGGTGTTCTTTATCTCT+TGG + Chr1:83962137-83962156 None:intergenic 35.0%
TCATCATTACATGTCAAGCT+AGG - Chr1:83962257-83962276 MsG0180004884.01.T01:intron 35.0%
TCTAAACATGCAGACTTTAC+TGG - Chr1:83963772-83963791 MsG0180004884.01.T01:five_prime_UTR 35.0%
TGAAATGGTTAACCAAGCAA+TGG - Chr1:83963200-83963219 MsG0180004884.01.T01:intron 35.0%
TGACACAAGAGTCATATTCA+AGG + Chr1:83963292-83963311 None:intergenic 35.0%
TTGCTTGGTTAACCATTTCA+GGG + Chr1:83963200-83963219 None:intergenic 35.0%
TTTACTGAACTCTAACTAGG+TGG - Chr1:83963432-83963451 MsG0180004884.01.T01:CDS 35.0%
TTTCATTGTATCTCAGGACA+TGG - Chr1:83962940-83962959 MsG0180004884.01.T01:intron 35.0%
! ATTGTTGTGTTTTGTGTTGC+AGG - Chr1:83962067-83962086 MsG0180004884.01.T01:CDS 35.0%
! ATTTTGAATGCTGGGTTTTG+GGG + Chr1:83961780-83961799 None:intergenic 35.0%
! GAATTTTGAATGCTGGGTTT+TGG + Chr1:83961782-83961801 None:intergenic 35.0%
! TGGAATCTCTTGTATGCTTT+TGG - Chr1:83962987-83963006 MsG0180004884.01.T01:intron 35.0%
! TTCGAGCATTTTGTTAGACA+AGG - Chr1:83963128-83963147 MsG0180004884.01.T01:intron 35.0%
!! CTCCTTCTTTAAGATGCTTT+GGG + Chr1:83961854-83961873 None:intergenic 35.0%
!! GCATGCTGTAGATATTTTGA+TGG - Chr1:83963032-83963051 MsG0180004884.01.T01:intron 35.0%
!! GGAGAGAATATTTTCAAGCT+CGG - Chr1:83961870-83961889 MsG0180004884.01.T01:intron 35.0%
!! TCCTTCTTTAAGATGCTTTG+GGG + Chr1:83961853-83961872 None:intergenic 35.0%
!! TCTCCTTCTTTAAGATGCTT+TGG + Chr1:83961855-83961874 None:intergenic 35.0%
ACGGTCTGCATGTCTTTAAA+AGG - Chr1:83962890-83962909 MsG0180004884.01.T01:intron 40.0%
AGCATAGAGGTCAAAAAGAC+AGG + Chr1:83963689-83963708 None:intergenic 40.0%
AGCCTCCTGTGTAAAAAACA+GGG - Chr1:83962748-83962767 MsG0180004884.01.T01:intron 40.0%
AGGTCAAAAAGACAGGATGA+AGG + Chr1:83963682-83963701 None:intergenic 40.0%
ATACCACATCGGATATGGAA+TGG + Chr1:83961980-83961999 None:intergenic 40.0%
CATTCAAAATTCCTCACCGA+TGG - Chr1:83961790-83961809 MsG0180004884.01.T01:intron 40.0%
CCCAAACACTTAACTTTCCA+GGG - Chr1:83963619-83963638 MsG0180004884.01.T01:CDS 40.0%
CCCTGGAAAGTTAAGTGTTT+GGG + Chr1:83963622-83963641 None:intergenic 40.0%
CCTGTGTAAAAAACAGGGTA+AGG - Chr1:83962753-83962772 MsG0180004884.01.T01:intron 40.0%
GATGATCCATGTCAATTCCA+TGG + Chr1:83963260-83963279 None:intergenic 40.0%
GCTGTGTACAATACACCAAA+TGG - Chr1:83962775-83962794 MsG0180004884.01.T01:intron 40.0%
GTGATGCATGTTCGGTAAAT+GGG + Chr1:83961678-83961697 None:intergenic 40.0%
GTGTACAATACACCAAATGG+TGG - Chr1:83962778-83962797 MsG0180004884.01.T01:intron 40.0%
TACCACATCGGATATGGAAT+GGG + Chr1:83961979-83961998 None:intergenic 40.0%
TCATCAGTTGTTGCTCTTGT+TGG - Chr1:83963873-83963892 MsG0180004884.01.T01:five_prime_UTR 40.0%
TCCCTGGAAAGTTAAGTGTT+TGG + Chr1:83963623-83963642 None:intergenic 40.0%
TCTGATTGAAAGAAACACGG+TGG + Chr1:83961741-83961760 None:intergenic 40.0%
TGTGATGCATGTTCGGTAAA+TGG + Chr1:83961679-83961698 None:intergenic 40.0%
TGTGTTACCACATACCACAT+CGG + Chr1:83961991-83962010 None:intergenic 40.0%
TTTGTGTTGCAGGTTCTTAG+TGG - Chr1:83962077-83962096 MsG0180004884.01.T01:CDS 40.0%
! AATTGTTGTCTAGGGTTTCG+TGG + Chr1:83961598-83961617 None:intergenic 40.0%
! ATTGTTGTCTAGGGTTTCGT+GGG + Chr1:83961597-83961616 None:intergenic 40.0%
! GGTGAGGAATTTTGAATGCT+GGG + Chr1:83961788-83961807 None:intergenic 40.0%
! GTAACCGGTAAATGCTTTTC+CGG + Chr1:83961922-83961941 None:intergenic 40.0%
! TTTGAGACTTTAGGAGCTCT+TGG - Chr1:83962967-83962986 MsG0180004884.01.T01:intron 40.0%
!! CCTTCTTTAAGATGCTTTGG+GGG + Chr1:83961852-83961871 None:intergenic 40.0%
!! GGTTGATGAAAGCAAATGCA+TGG - Chr1:83963829-83963848 MsG0180004884.01.T01:five_prime_UTR 40.0%
!! TCTTGTATGCTTTTGGCTAC+CGG - Chr1:83962994-83963013 MsG0180004884.01.T01:intron 40.0%
!! TTTTGAATGCTGGGTTTTGG+GGG + Chr1:83961779-83961798 None:intergenic 40.0%
!!! CCTTACCCTGTTTTTTACAC+AGG + Chr1:83962756-83962775 None:intergenic 40.0%
!!! TACCCTGTTTTTTACACAGG+AGG + Chr1:83962753-83962772 None:intergenic 40.0%
AAACAAGCTGGTGAAAGGCA+AGG - Chr1:83963801-83963820 MsG0180004884.01.T01:five_prime_UTR 45.0%
ACACCACCATGGAATTGACA+TGG - Chr1:83963251-83963270 MsG0180004884.01.T01:CDS 45.0%
ACCACATACCACATCGGATA+TGG + Chr1:83961985-83962004 None:intergenic 45.0%
ATTCCTCACCGATGGCATTT+CGG - Chr1:83961798-83961817 MsG0180004884.01.T01:intron 45.0%
CAGCCTCCTGTGTAAAAAAC+AGG - Chr1:83962747-83962766 MsG0180004884.01.T01:intron 45.0%
GAGAATGACCGAAATGCCAT+CGG + Chr1:83961809-83961828 None:intergenic 45.0%
GATCCATGTCAATTCCATGG+TGG + Chr1:83963257-83963276 None:intergenic 45.0%
GCCCAAACACTTAACTTTCC+AGG - Chr1:83963618-83963637 MsG0180004884.01.T01:CDS 45.0%
GTTGTTGCTCTTGTTGGAGT+TGG - Chr1:83963879-83963898 MsG0180004884.01.T01:five_prime_UTR 45.0%
TCCATATCCGATGTGGTATG+TGG - Chr1:83961981-83962000 MsG0180004884.01.T01:intron 45.0%
TCCGGTGGCTAAAGCAATAT+CGG + Chr1:83961904-83961923 None:intergenic 45.0%
! AAAGGCGTGTGATGCATGTT+CGG + Chr1:83961686-83961705 None:intergenic 45.0%
! AAGGTCATGGGTTGAAGTCA+TGG - Chr1:83962721-83962740 MsG0180004884.01.T01:intron 45.0%
! ATCTTTGTCACGTGACGGAA+AGG - Chr1:83962702-83962721 MsG0180004884.01.T01:intron 45.0%
! CAAATGGTGGAATCTCTTCC+CGG - Chr1:83962791-83962810 MsG0180004884.01.T01:intron 45.0%
! CCCCCAAAGCATCTTAAAGA+AGG - Chr1:83961849-83961868 MsG0180004884.01.T01:intron 45.0%
! CGGTGAGGAATTTTGAATGC+TGG + Chr1:83961789-83961808 None:intergenic 45.0%
! GCATTTACCGGTTACCTTTC+CGG - Chr1:83961927-83961946 MsG0180004884.01.T01:intron 45.0%
! TATGCACATGTGCCATTGCT+TGG + Chr1:83963215-83963234 None:intergenic 45.0%
!! CTAGACTCCTAAGAGTGTCT+TGG + Chr1:83962511-83962530 None:intergenic 45.0%
!! CTTGTATGCTTTTGGCTACC+GGG - Chr1:83962995-83963014 MsG0180004884.01.T01:intron 45.0%
!! TGCTCTTGTTGGAGTTGGTA+AGG - Chr1:83963884-83963903 MsG0180004884.01.T01:five_prime_UTR 45.0%
!! TTGTATGCTTTTGGCTACCG+GGG - Chr1:83962996-83963015 MsG0180004884.01.T01:intron 45.0%
!!! GGAGGTGTTTTGAAGAGGAA+AGG + Chr1:83961704-83961723 None:intergenic 45.0%
!!! TGGAAGGAGGTGTTTTGAAG+AGG + Chr1:83961709-83961728 None:intergenic 45.0%
!!! TTTGAATGCTGGGTTTTGGG+GGG + Chr1:83961778-83961797 None:intergenic 45.0%
AGCCTGATGCACTAAAGTCC+GGG + Chr1:83962812-83962831 None:intergenic 50.0%
ATGGCACATGTGCATAGCCA+CGG - Chr1:83963219-83963238 MsG0180004884.01.T01:intron 50.0%
CAGCCTGATGCACTAAAGTC+CGG + Chr1:83962813-83962832 None:intergenic 50.0%
CAGTTCCCTTTACACGAGCA+TGG - Chr1:83961488-83961507 MsG0180004884.01.T01:CDS 50.0%
CCATCCACCAAGACACTCTT+AGG - Chr1:83962501-83962520 MsG0180004884.01.T01:intron 50.0%
CGGGAAGAGATTCCACCATT+TGG + Chr1:83962793-83962812 None:intergenic 50.0%
CTCGTGTAAAGGGAACTGTG+TGG + Chr1:83961486-83961505 None:intergenic 50.0%
GAATGGAAGCCAAAGAGCCA+AGG + Chr1:83963660-83963679 None:intergenic 50.0%
GCCGATATTGCTTTAGCCAC+CGG - Chr1:83961900-83961919 MsG0180004884.01.T01:intron 50.0%
GGATATGGAATGGGCAGCAT+CGG + Chr1:83961970-83961989 None:intergenic 50.0%
GGTACTACCGGAAAGGTAAC+CGG + Chr1:83961937-83961956 None:intergenic 50.0%
GGTGGCTAAAGCAATATCGG+CGG + Chr1:83961901-83961920 None:intergenic 50.0%
GTTCGCCATGCTCGTGTAAA+GGG + Chr1:83961496-83961515 None:intergenic 50.0%
TAATCGCGGTACTACCGGAA+AGG + Chr1:83961944-83961963 None:intergenic 50.0%
TCGCGGTGTAGTTATCAGTG+AGG - Chr1:83962474-83962493 MsG0180004884.01.T01:intron 50.0%
TGAAAGAAACACGGTGGCTC+CGG + Chr1:83961735-83961754 None:intergenic 50.0%
TGCCCATTCCATATCCGATG+TGG - Chr1:83961974-83961993 MsG0180004884.01.T01:intron 50.0%
TGTTCGCCATGCTCGTGTAA+AGG + Chr1:83961497-83961516 None:intergenic 50.0%
TTCCCGGACTTTAGTGCATC+AGG - Chr1:83962807-83962826 MsG0180004884.01.T01:intron 50.0%
! ACCGGTAAATGCTTTTCCGG+TGG + Chr1:83961919-83961938 None:intergenic 50.0%
! ATGCAGGGATTTTCCTCAGC+TGG - Chr1:83963174-83963193 MsG0180004884.01.T01:intron 50.0%
! TCACGTGACGGAAAGGTCAT+GGG - Chr1:83962709-83962728 MsG0180004884.01.T01:intron 50.0%
!! CCTAAGAGTGTCTTGGTGGA+TGG + Chr1:83962504-83962523 None:intergenic 50.0%
!! GACTCCTAAGAGTGTCTTGG+TGG + Chr1:83962508-83962527 None:intergenic 50.0%
!! GCCACCGGAAAAGCATTTAC+CGG - Chr1:83961915-83961934 MsG0180004884.01.T01:intron 50.0%
!! TGTATGCTTTTGGCTACCGG+GGG - Chr1:83962997-83963016 MsG0180004884.01.T01:intron 50.0%
AAAACACCTCCTTCCACCGC+CGG - Chr1:83961713-83961732 MsG0180004884.01.T01:intron 55.0%
AACCATTTCAGGGCCAGCTG+AGG + Chr1:83963190-83963209 None:intergenic 55.0%
AAGCCAAAGAGCCAAGGCCA+TGG + Chr1:83963654-83963673 None:intergenic 55.0%
CATGTGCATAGCCACGGACA+AGG - Chr1:83963225-83963244 MsG0180004884.01.T01:intron 55.0%
CTGGTGAAAGGCAAGGCAGT+GGG - Chr1:83963808-83963827 MsG0180004884.01.T01:five_prime_UTR 55.0%
GGCGATAATCGCGGTACTAC+CGG + Chr1:83961949-83961968 None:intergenic 55.0%
TGACCGAAATGCCATCGGTG+AGG + Chr1:83961804-83961823 None:intergenic 55.0%
TGGCGAACAACATCGCGTTC+AGG - Chr1:83961508-83961527 MsG0180004884.01.T01:CDS 55.0%
TTCCAGGGAATGTGTCGCCA+TGG - Chr1:83963634-83963653 MsG0180004884.01.T01:CDS 55.0%
TTCCTCAGCTGGCCCTGAAA+TGG - Chr1:83963185-83963204 MsG0180004884.01.T01:intron 55.0%
! GTCACGTGACGGAAAGGTCA+TGG - Chr1:83962708-83962727 MsG0180004884.01.T01:intron 55.0%
AAGAAACACGGTGGCTCCGG+CGG + Chr1:83961732-83961751 None:intergenic 60.0%
ATGCCATGCAATGCCACCCC+CGG + Chr1:83963016-83963035 None:intergenic 60.0%
GCTGGTGAAAGGCAAGGCAG+TGG - Chr1:83963807-83963826 MsG0180004884.01.T01:five_prime_UTR 60.0%
GGAATGTGTCGCCATGGCCT+TGG - Chr1:83963640-83963659 MsG0180004884.01.T01:CDS 60.0%
GTGCATAGCCACGGACAAGG+CGG - Chr1:83963228-83963247 MsG0180004884.01.T01:CDS 60.0%
! TCGCCATGGCCTTGGCTCTT+TGG - Chr1:83963648-83963667 MsG0180004884.01.T01:CDS 60.0%
!! ATGCTTTTGGCTACCGGGGG+TGG - Chr1:83963000-83963019 MsG0180004884.01.T01:intron 60.0%
!! GGCAGCATCGGCGATAATCG+CGG + Chr1:83961958-83961977 None:intergenic 60.0%
CTACCGGGGGTGGCATTGCA+TGG - Chr1:83963010-83963029 MsG0180004884.01.T01:intron 65.0%
GGACAAGGCGGACACCACCA+TGG - Chr1:83963240-83963259 MsG0180004884.01.T01:CDS 65.0%
GGCCATGGCGACACATTCCC+TGG + Chr1:83963639-83963658 None:intergenic 65.0%
AAACACGGTGGCTCCGGCGG+TGG + Chr1:83961729-83961748 None:intergenic 70.0%
GGTGGTGTCCGCCTTGTCCG+TGG + Chr1:83963239-83963258 None:intergenic 70.0%
ACGGTGGCTCCGGCGGTGGA+AGG + Chr1:83961725-83961744 None:intergenic 75.0%
GTGGCTCCGGCGGTGGAAGG+AGG + Chr1:83961722-83961741 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 83961453 83963932 83961453 ID=MsG0180004884.01;Name=MsG0180004884.01
Chr1 mRNA 83961453 83963932 83961453 ID=MsG0180004884.01.T01;Parent=MsG0180004884.01;Name=MsG0180004884.01.T01;_AED=0.43;_eAED=0.43;_QI=195|1|0.8|1|1|1|5|0|270
Chr1 exon 83961453 83961600 83961453 ID=MsG0180004884.01.T01:exon:9111;Parent=MsG0180004884.01.T01
Chr1 exon 83962057 83962205 83962057 ID=MsG0180004884.01.T01:exon:9110;Parent=MsG0180004884.01.T01
Chr1 exon 83962332 83962429 83962332 ID=MsG0180004884.01.T01:exon:9109;Parent=MsG0180004884.01.T01
Chr1 exon 83963227 83963296 83963227 ID=MsG0180004884.01.T01:exon:9108;Parent=MsG0180004884.01.T01
Chr1 exon 83963390 83963932 83963390 ID=MsG0180004884.01.T01:exon:9107;Parent=MsG0180004884.01.T01
Chr1 five_prime_UTR 83963738 83963932 83963738 ID=MsG0180004884.01.T01:five_prime_utr;Parent=MsG0180004884.01.T01
Chr1 CDS 83963390 83963737 83963390 ID=MsG0180004884.01.T01:cds;Parent=MsG0180004884.01.T01
Chr1 CDS 83963227 83963296 83963227 ID=MsG0180004884.01.T01:cds;Parent=MsG0180004884.01.T01
Chr1 CDS 83962332 83962429 83962332 ID=MsG0180004884.01.T01:cds;Parent=MsG0180004884.01.T01
Chr1 CDS 83962057 83962205 83962057 ID=MsG0180004884.01.T01:cds;Parent=MsG0180004884.01.T01
Chr1 CDS 83961453 83961600 83961453 ID=MsG0180004884.01.T01:cds;Parent=MsG0180004884.01.T01
Gene Sequence

>MsG0180004884.01.T01

ATGGGATTTAGATTCAACAATCTGAATCCCATTTACCGAACATGCATCACACGCCTTTCCTCTTCAAAACACCTCCTTCCACCGCCGGAGCCACCGTGTTTCTTTCAATCAGAAGCTTCTTTCATTTCCCCCCAAAACCCAGCATTCAAAATTCCTCACCGATGGCATTTCGGTCATTCTCACGATCATGATCATCATCATCCCCCAAAGCATCTTAAAGAAGGAGAGAATATTTTCAAGCTCGGTCTCGCCGCCGATATTGCTTTAGCCACCGGAAAAGCATTTACCGGTTACCTTTCCGGTAGTACCGCGATTATCGCCGATGCTGCCCATTCCATATCCGATGTGGTTCTTAGTGGCATAGCTTTGTTGTCTTTTAAAGTTGCAAAGGCTCCAAGAGATAAAGAACACCCATATGGACATGGTAAATTTGAGACTTTAGGAGCTCTTGGAATCTCTTGTATGCTTTTGGCTACCGGGGGTGGCATTGCATGGCATGCTGTAGATATTTTGATGGGATTTTCCTCAGCTGGCCCTGAAATGGTTAACCAAGCAATGGCACATGTGCATAGCCACGGACAAGGCGGACACCACCATGGAATTGACATGGATCATCCTATACTTGCCTTGAATATGACTCTTGTGTCAATAGGTGTTAAAGAAGGACTTTACTGGATAACAAAACAAGCTGGTGAAAGGCAAGGCAGTGGGTTGATGAAAGCAAATGCATGGCATCATCGTGCAGATGCAATTTCATCAGTTGTTGCTCTTGTTGGAGTTGGTAAGGAATTTGAATATTTGAAAGGTCTCTAG
Protein sequence

>MsG0180004884.01.T01

MGFRFNNLNPIYRTCITRLSSSKHLLPPPEPPCFFQSEASFISPQNPAFKIPHRWHFGHSHDHDHHHPPKHLKEGENIFKLGLAADIALATGKAFTGYLSGSTAIIADAAHSISDVVLSGIALLSFKVAKAPRDKEHPYGHGKFETLGALGISCMLLATGGGIAWHAVDILMGFSSAGPEMVNQAMAHVHSHGQGGHHHGIDMDHPILALNMTLVSIGVKEGLYWITKQAGERQGSGLMKANAWHHRADAISSVVALVGVGKEFEYLKGL*