Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005120.01.T01 | AES62102.2 | 98.039 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 5.23E-24 | 94.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005120.01.T01 | G7I4Y9 | 98.039 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 2.50e-24 | 94.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004518.01 | MsG0180005120.01 | 0.812097 | 5.032069e-51 | 3.170172e-48 |
| MsG0180005120.01 | MsG0680031146.01 | 0.808671 | 2.762312e-50 | 1.588990e-47 |
| MsG0180005120.01 | MsG0780041262.01 | 0.828490 | 8.733011e-55 | 8.693873e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005120.01.T01 | MTR_1g093960 | 98.039 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 6.33e-28 | 94.7 |
| MsG0180005120.01.T01 | MTR_1g093985 | 98.039 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 6.33e-28 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGGAGTAGCTTCTTTCT+TGG | 0.263984 | 1:-87286787 | None:intergenic |
| AGCTTTCTTTGAAGCTGATT+TGG | 0.275824 | 1:-87286832 | None:intergenic |
| TGACCCTTATCACTAGCTTT+AGG | 0.299140 | 1:-87286804 | None:intergenic |
| TCAAGATCTTAATAAGAAAA+AGG | 0.310643 | 1:+87286866 | MsG0180005120.01.T01:CDS |
| TACTCCTAAAGCTAGTGATA+AGG | 0.421532 | 1:+87286800 | MsG0180005120.01.T01:CDS |
| ACTCCTAAAGCTAGTGATAA+GGG | 0.443378 | 1:+87286801 | MsG0180005120.01.T01:CDS |
| AACAGGTTTATCAGCCTTCA+AGG | 0.447580 | 1:-87286742 | None:intergenic |
| GTGCAAAATGGCATCCTTGA+AGG | 0.478600 | 1:+87286728 | None:intergenic |
| TTGAAGGCTGATAAACCTGT+TGG | 0.591373 | 1:+87286744 | MsG0180005120.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAGATCTTAATAAGAAAA+AGG | + | Chr1:87286866-87286885 | MsG0180005120.01.T01:CDS | 20.0% |
| !!! | TTTTTCTTATTAAGATCTTG+AGG | - | Chr1:87286867-87286886 | None:intergenic | 20.0% |
| ACTCCTAAAGCTAGTGATAA+GGG | + | Chr1:87286801-87286820 | MsG0180005120.01.T01:CDS | 35.0% | |
| TACTCCTAAAGCTAGTGATA+AGG | + | Chr1:87286800-87286819 | MsG0180005120.01.T01:CDS | 35.0% | |
| TTTAGGAGTAGCTTCTTTCT+TGG | - | Chr1:87286790-87286809 | None:intergenic | 35.0% | |
| !! | AGCTTTCTTTGAAGCTGATT+TGG | - | Chr1:87286835-87286854 | None:intergenic | 35.0% |
| AACAGGTTTATCAGCCTTCA+AGG | - | Chr1:87286745-87286764 | None:intergenic | 40.0% | |
| TTGAAGGCTGATAAACCTGT+TGG | + | Chr1:87286744-87286763 | MsG0180005120.01.T01:CDS | 40.0% | |
| !! | TGACCCTTATCACTAGCTTT+AGG | - | Chr1:87286807-87286826 | None:intergenic | 40.0% |
| ! | CTGTTGGCACCCAACTTTTT+GGG | + | Chr1:87286760-87286779 | MsG0180005120.01.T01:CDS | 45.0% |
| CCAAAAAGTTGGGTGCCAAC+AGG | - | Chr1:87286762-87286781 | None:intergenic | 50.0% | |
| CTTGGCCTGCCCAAAAAGTT+GGG | - | Chr1:87286772-87286791 | None:intergenic | 50.0% | |
| TCTTGGCCTGCCCAAAAAGT+TGG | - | Chr1:87286773-87286792 | None:intergenic | 50.0% | |
| ! | CCTGTTGGCACCCAACTTTT+TGG | + | Chr1:87286759-87286778 | MsG0180005120.01.T01:CDS | 50.0% |
| !! | TGGCACCCAACTTTTTGGGC+AGG | + | Chr1:87286764-87286783 | MsG0180005120.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 87286735 | 87286896 | 87286735 | ID=MsG0180005120.01;Name=MsG0180005120.01 |
| Chr1 | mRNA | 87286735 | 87286896 | 87286735 | ID=MsG0180005120.01.T01;Parent=MsG0180005120.01;Name=MsG0180005120.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|53 |
| Chr1 | exon | 87286735 | 87286896 | 87286735 | ID=MsG0180005120.01.T01:exon:47724;Parent=MsG0180005120.01.T01 |
| Chr1 | CDS | 87286735 | 87286896 | 87286735 | ID=MsG0180005120.01.T01:cds;Parent=MsG0180005120.01.T01 |
| Gene Sequence |
| Protein sequence |
