Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005222.01.T01 | RHN81508.1 | 96.875 | 192 | 6 | 0 | 1 | 192 | 137 | 328 | 9.37E-137 | 395 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005222.01.T01 | Q921I1 | 35.838 | 173 | 94 | 8 | 2 | 171 | 465 | 623 | 1.88E-19 | 88.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005222.01.T01 | A0A396JZQ2 | 96.875 | 192 | 6 | 0 | 1 | 192 | 137 | 328 | 4.48e-137 | 395 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005222.01 | MsG0180005241.01 | 0.892883 | 1.051874e-74 | 9.794052e-71 |
| MsG0180005222.01 | MsG0580029251.01 | 0.809191 | 2.137791e-50 | 1.246943e-47 |
| MsG0180005222.01 | MsG0580029298.01 | 0.800832 | 1.197653e-48 | 5.632685e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005222.01.T01 | MTR_1g095450 | 96.859 | 191 | 6 | 0 | 1 | 191 | 137 | 327 | 1.16e-139 | 394 |
| MsG0180005222.01.T01 | MTR_0224s0020 | 97.143 | 35 | 1 | 0 | 157 | 191 | 1 | 35 | 1.68e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGTCAACAAAGTCTCTT+AGG | 0.218867 | 1:+88782345 | MsG0180005222.01.T01:CDS |
| TACTGCCTTGTTCTATTCTA+TGG | 0.274106 | 1:+88782304 | MsG0180005222.01.T01:CDS |
| AATGAAACCTGCAATGAAAT+AGG | 0.296785 | 1:+88782206 | MsG0180005222.01.T01:CDS |
| TGTGCCATTATCACATTTGC+AGG | 0.322187 | 1:-88782689 | None:intergenic |
| ATACAAGCCTATTTCATTGC+AGG | 0.329472 | 1:-88782213 | None:intergenic |
| GAGACTTTGTTGACCATGAT+TGG | 0.374237 | 1:-88782340 | None:intergenic |
| TTAGGTATCTTTGCCCTAAA+GGG | 0.379845 | 1:+88782458 | MsG0180005222.01.T01:intron |
| AACATTGACGAATACTAGTT+TGG | 0.381344 | 1:+88782751 | MsG0180005222.01.T01:CDS |
| GACATGCATTCTCTTCTTGT+TGG | 0.385917 | 1:-88782723 | None:intergenic |
| ACATGCATTCTCTTCTTGTT+GGG | 0.406977 | 1:-88782722 | None:intergenic |
| AGGTGTCTAGTAGAGGAGTT+GGG | 0.413895 | 1:+88782257 | MsG0180005222.01.T01:CDS |
| TAGGTGTCTAGTAGAGGAGT+TGG | 0.421638 | 1:+88782256 | MsG0180005222.01.T01:CDS |
| TGTTCCTGCAAATGTGATAA+TGG | 0.432281 | 1:+88782685 | MsG0180005222.01.T01:CDS |
| AATGAAATAGGCTTGTATTC+TGG | 0.456061 | 1:+88782218 | MsG0180005222.01.T01:CDS |
| TTTAGGTATCTTTGCCCTAA+AGG | 0.460465 | 1:+88782457 | MsG0180005222.01.T01:intron |
| GGTTGTTGCGAATATCGATT+GGG | 0.462387 | 1:-88782790 | None:intergenic |
| GTTGACCATGATTGGTTATG+AGG | 0.462653 | 1:-88782332 | None:intergenic |
| GCCTTGTTCTATTCTATGGA+GGG | 0.487961 | 1:+88782308 | MsG0180005222.01.T01:CDS |
| GGAGGTTACTCCACAGCAGC+AGG | 0.505464 | 1:+88782038 | MsG0180005222.01.T01:CDS |
| AGGTTGTTGCGAATATCGAT+TGG | 0.533382 | 1:-88782791 | None:intergenic |
| ATGGTCAACAAAGTCTCTTA+GGG | 0.536352 | 1:+88782346 | MsG0180005222.01.T01:CDS |
| GTAGTTCCATCCTGCTGCTG+TGG | 0.542388 | 1:-88782048 | None:intergenic |
| AAAACTTACGAACAATCTGC+AGG | 0.544949 | 1:-88782505 | None:intergenic |
| ATTGACGAATACTAGTTTGG+TGG | 0.551045 | 1:+88782754 | MsG0180005222.01.T01:CDS |
| TGCCTTGTTCTATTCTATGG+AGG | 0.553128 | 1:+88782307 | MsG0180005222.01.T01:CDS |
| AGGGACCTCATAACCAATCA+TGG | 0.554719 | 1:+88782327 | MsG0180005222.01.T01:CDS |
| TCAAAACCTTCAACCTCTGA+TGG | 0.557623 | 1:-88782158 | None:intergenic |
| ATCAGTTTAATGGACTTCAA+AGG | 0.562705 | 1:+88781999 | None:intergenic |
| GCAACAACCTATTTAGTCCA+AGG | 0.567581 | 1:+88782804 | MsG0180005222.01.T01:CDS |
| TGTGCACCATCAGAGGTTGA+AGG | 0.574026 | 1:+88782152 | MsG0180005222.01.T01:CDS |
| GGCTTGTATTCTGGTCACTC+AGG | 0.574907 | 1:+88782227 | MsG0180005222.01.T01:CDS |
| TTTGATACATGTAATGTATG+TGG | 0.576499 | 1:+88782176 | MsG0180005222.01.T01:CDS |
| AGGTATCTTTGCCCTAAAGG+GGG | 0.576544 | 1:+88782460 | MsG0180005222.01.T01:intron |
| AAAGGTCACAAGTCTTGCCA+TGG | 0.587608 | 1:+88782017 | MsG0180005222.01.T01:CDS |
| ACGACTTTGTGCACCATCAG+AGG | 0.587718 | 1:+88782145 | MsG0180005222.01.T01:CDS |
| TAGGTATCTTTGCCCTAAAG+GGG | 0.593324 | 1:+88782459 | MsG0180005222.01.T01:intron |
| GTTACTCCACAGCAGCAGGA+TGG | 0.625433 | 1:+88782042 | MsG0180005222.01.T01:CDS |
| CTGCTGTGGAGTAACCTCCA+TGG | 0.636623 | 1:-88782034 | None:intergenic |
| GGTCACAAGTCTTGCCATGG+AGG | 0.651029 | 1:+88782020 | MsG0180005222.01.T01:CDS |
| TCCCTCCATAGAATAGAACA+AGG | 0.696491 | 1:-88782309 | None:intergenic |
| GTTGTTGCGAATATCGATTG+GGG | 0.707340 | 1:-88782789 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTTAATGTGATTGAT+GGG | - | Chr1:88782074-88782093 | None:intergenic | 15.0% |
| !!! | TAAATTTTTAATGTGATTGA+TGG | - | Chr1:88782075-88782094 | None:intergenic | 15.0% |
| !! | AGTTATGTTAAAATGATAAC+AGG | - | Chr1:88782629-88782648 | None:intergenic | 20.0% |
| !! | TGATGAAAAAATGAATGATA+AGG | + | Chr1:88782100-88782119 | MsG0180005222.01.T01:CDS | 20.0% |
| !!! | AATTTTTAATGTGATTGATG+GGG | - | Chr1:88782073-88782092 | None:intergenic | 20.0% |
| !!! | TCAATCAATTGAATGATTTT+AGG | + | Chr1:88782440-88782459 | MsG0180005222.01.T01:intron | 20.0% |
| ! | CAATTGATTGATATTAGTTG+AGG | - | Chr1:88782431-88782450 | None:intergenic | 25.0% |
| ! | GAAAAAAAATCTTACACCTA+TGG | - | Chr1:88782560-88782579 | None:intergenic | 25.0% |
| ! | TTTGATACATGTAATGTATG+TGG | + | Chr1:88782176-88782195 | MsG0180005222.01.T01:CDS | 25.0% |
| !!! | CATTTTAACATAACTAGAGT+TGG | + | Chr1:88782634-88782653 | MsG0180005222.01.T01:intron | 25.0% |
| AACATTGACGAATACTAGTT+TGG | + | Chr1:88782751-88782770 | MsG0180005222.01.T01:CDS | 30.0% | |
| AATGAAACCTGCAATGAAAT+AGG | + | Chr1:88782206-88782225 | MsG0180005222.01.T01:CDS | 30.0% | |
| AATGAAATAGGCTTGTATTC+TGG | + | Chr1:88782218-88782237 | MsG0180005222.01.T01:CDS | 30.0% | |
| CATCGCATGAAAAGTTTATT+AGG | - | Chr1:88782391-88782410 | None:intergenic | 30.0% | |
| CATGAAAAGTTTATTAGGCA+AGG | - | Chr1:88782386-88782405 | None:intergenic | 30.0% | |
| TGCAATTAGAAACATGCATT+AGG | - | Chr1:88782585-88782604 | None:intergenic | 30.0% | |
| AAAACTTACGAACAATCTGC+AGG | - | Chr1:88782508-88782527 | None:intergenic | 35.0% | |
| AACAGTTCCAAACCTGATTA+AGG | - | Chr1:88782669-88782688 | None:intergenic | 35.0% | |
| ACATGCATTCTCTTCTTGTT+GGG | - | Chr1:88782725-88782744 | None:intergenic | 35.0% | |
| ATACAAGCCTATTTCATTGC+AGG | - | Chr1:88782216-88782235 | None:intergenic | 35.0% | |
| ATGGTCAACAAAGTCTCTTA+GGG | + | Chr1:88782346-88782365 | MsG0180005222.01.T01:CDS | 35.0% | |
| TGTTCCTGCAAATGTGATAA+TGG | + | Chr1:88782685-88782704 | MsG0180005222.01.T01:CDS | 35.0% | |
| TTAGGTATCTTTGCCCTAAA+GGG | + | Chr1:88782458-88782477 | MsG0180005222.01.T01:intron | 35.0% | |
| TTTAGGTATCTTTGCCCTAA+AGG | + | Chr1:88782457-88782476 | MsG0180005222.01.T01:intron | 35.0% | |
| TTTCATGCGATGCATAAAGT+AGG | + | Chr1:88782399-88782418 | MsG0180005222.01.T01:intron | 35.0% | |
| ! | ATTGACGAATACTAGTTTGG+TGG | + | Chr1:88782754-88782773 | MsG0180005222.01.T01:CDS | 35.0% |
| ! | TACTGCCTTGTTCTATTCTA+TGG | + | Chr1:88782304-88782323 | MsG0180005222.01.T01:CDS | 35.0% |
| AGGTTGTTGCGAATATCGAT+TGG | - | Chr1:88782794-88782813 | None:intergenic | 40.0% | |
| CACATGTTTGTGTTTGCCAT+AGG | + | Chr1:88782541-88782560 | MsG0180005222.01.T01:intron | 40.0% | |
| CATGGTCAACAAAGTCTCTT+AGG | + | Chr1:88782345-88782364 | MsG0180005222.01.T01:CDS | 40.0% | |
| GACATGCATTCTCTTCTTGT+TGG | - | Chr1:88782726-88782745 | None:intergenic | 40.0% | |
| GAGACTTTGTTGACCATGAT+TGG | - | Chr1:88782343-88782362 | None:intergenic | 40.0% | |
| GCAACAACCTATTTAGTCCA+AGG | + | Chr1:88782804-88782823 | MsG0180005222.01.T01:CDS | 40.0% | |
| GCTTCATCCTTAATCAGGTT+TGG | + | Chr1:88782659-88782678 | MsG0180005222.01.T01:intron | 40.0% | |
| TAGGTATCTTTGCCCTAAAG+GGG | + | Chr1:88782459-88782478 | MsG0180005222.01.T01:intron | 40.0% | |
| TCAAAACCTTCAACCTCTGA+TGG | - | Chr1:88782161-88782180 | None:intergenic | 40.0% | |
| TCCCTCCATAGAATAGAACA+AGG | - | Chr1:88782312-88782331 | None:intergenic | 40.0% | |
| TGGTTGCTTCATCCTTAATC+AGG | + | Chr1:88782654-88782673 | MsG0180005222.01.T01:intron | 40.0% | |
| ! | GCCTTGTTCTATTCTATGGA+GGG | + | Chr1:88782308-88782327 | MsG0180005222.01.T01:CDS | 40.0% |
| ! | GGTTGTTGCGAATATCGATT+GGG | - | Chr1:88782793-88782812 | None:intergenic | 40.0% |
| ! | GTTGACCATGATTGGTTATG+AGG | - | Chr1:88782335-88782354 | None:intergenic | 40.0% |
| ! | GTTGTTGCGAATATCGATTG+GGG | - | Chr1:88782792-88782811 | None:intergenic | 40.0% |
| ! | TGCCTTGTTCTATTCTATGG+AGG | + | Chr1:88782307-88782326 | MsG0180005222.01.T01:CDS | 40.0% |
| ! | TGTGCCATTATCACATTTGC+AGG | - | Chr1:88782692-88782711 | None:intergenic | 40.0% |
| !! | AGCTTTTAGGTGTCTAGTAG+AGG | + | Chr1:88782250-88782269 | MsG0180005222.01.T01:CDS | 40.0% |
| !! | GGAGACATTGCTTTTGTCAA+GGG | + | Chr1:88782278-88782297 | MsG0180005222.01.T01:CDS | 40.0% |
| AAAGGTCACAAGTCTTGCCA+TGG | + | Chr1:88782017-88782036 | MsG0180005222.01.T01:CDS | 45.0% | |
| AGGGACCTCATAACCAATCA+TGG | + | Chr1:88782327-88782346 | MsG0180005222.01.T01:CDS | 45.0% | |
| AGGTATCTTTGCCCTAAAGG+GGG | + | Chr1:88782460-88782479 | MsG0180005222.01.T01:intron | 45.0% | |
| AGGTGTCTAGTAGAGGAGTT+GGG | + | Chr1:88782257-88782276 | MsG0180005222.01.T01:CDS | 45.0% | |
| ATTTCTCTGCAACCCCCTTT+AGG | - | Chr1:88782475-88782494 | None:intergenic | 45.0% | |
| TAGGTGTCTAGTAGAGGAGT+TGG | + | Chr1:88782256-88782275 | MsG0180005222.01.T01:CDS | 45.0% | |
| TTTCTCTGCAACCCCCTTTA+GGG | - | Chr1:88782474-88782493 | None:intergenic | 45.0% | |
| !! | GGGAGACATTGCTTTTGTCA+AGG | + | Chr1:88782277-88782296 | MsG0180005222.01.T01:CDS | 45.0% |
| ACGACTTTGTGCACCATCAG+AGG | + | Chr1:88782145-88782164 | MsG0180005222.01.T01:CDS | 50.0% | |
| GGCTTGTATTCTGGTCACTC+AGG | + | Chr1:88782227-88782246 | MsG0180005222.01.T01:CDS | 50.0% | |
| GGGGGTTGCAGAGAAATCAA+TGG | + | Chr1:88782478-88782497 | MsG0180005222.01.T01:CDS | 50.0% | |
| TGTGCACCATCAGAGGTTGA+AGG | + | Chr1:88782152-88782171 | MsG0180005222.01.T01:CDS | 50.0% | |
| ! | CTGGTCACTCAGGAGCTTTT+AGG | + | Chr1:88782237-88782256 | MsG0180005222.01.T01:CDS | 50.0% |
| CTGCTGTGGAGTAACCTCCA+TGG | - | Chr1:88782037-88782056 | None:intergenic | 55.0% | |
| GGTCACAAGTCTTGCCATGG+AGG | + | Chr1:88782020-88782039 | MsG0180005222.01.T01:CDS | 55.0% | |
| GTAGTTCCATCCTGCTGCTG+TGG | - | Chr1:88782051-88782070 | None:intergenic | 55.0% | |
| GTTACTCCACAGCAGCAGGA+TGG | + | Chr1:88782042-88782061 | MsG0180005222.01.T01:CDS | 55.0% | |
| GGAGGTTACTCCACAGCAGC+AGG | + | Chr1:88782038-88782057 | MsG0180005222.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 88782008 | 88782829 | 88782008 | ID=MsG0180005222.01;Name=MsG0180005222.01 |
| Chr1 | mRNA | 88782008 | 88782829 | 88782008 | ID=MsG0180005222.01.T01;Parent=MsG0180005222.01;Name=MsG0180005222.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|0.66|3|0|192 |
| Chr1 | exon | 88782008 | 88782375 | 88782008 | ID=MsG0180005222.01.T01:exon:11051;Parent=MsG0180005222.01.T01 |
| Chr1 | exon | 88782462 | 88782518 | 88782462 | ID=MsG0180005222.01.T01:exon:11052;Parent=MsG0180005222.01.T01 |
| Chr1 | exon | 88782676 | 88782829 | 88782676 | ID=MsG0180005222.01.T01:exon:11053;Parent=MsG0180005222.01.T01 |
| Chr1 | CDS | 88782008 | 88782375 | 88782008 | ID=MsG0180005222.01.T01:cds;Parent=MsG0180005222.01.T01 |
| Chr1 | CDS | 88782462 | 88782518 | 88782462 | ID=MsG0180005222.01.T01:cds;Parent=MsG0180005222.01.T01 |
| Chr1 | CDS | 88782676 | 88782829 | 88782676 | ID=MsG0180005222.01.T01:cds;Parent=MsG0180005222.01.T01 |
| Gene Sequence |
| Protein sequence |
