AlfalfaGEDB | MsG0180005385.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005385.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005385.01.T01	XP_003592088.1	83.544	237	7	3	1	205	1	237	5.69E-128	370

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005385.01.T01	G7IBY5	83.544	237	7	3	1	205	1	237	2.72e-128	370

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005385.01	MsG0480023008.01	0.828023	1.132185e-54	1.111881e-51
MsG0180005385.01	MsG0880046187.01	0.809605	1.742205e-50	1.027511e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005385.01.T01	MTR_1g098580	83.544	237	7	3	1	205	1	237	6.90e-132	370
MsG0180005385.01.T01	MTR_1g098580	86.385	213	2	2	1	186	1	213	7.92e-123	347
MsG0180005385.01.T01	MTR_1g098580	72.152	237	8	4	1	205	1	211	1.35e-96	280

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005385.01.T01	AT1G21090	46.729	214	78	4	2	187	10	215	5.43e-53	170
MsG0180005385.01.T01	AT1G21090	46.729	214	78	4	2	187	10	215	1.83e-52	168

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Find 74 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGGGAGAAAATGGGGT+AGG	0.562091	1:-91127076	MsG0180005385.01.T01:three_prime_UTR
AAGAACCCCACTGTTTCCAT+TGG	0.557299	1:-91128349	MsG0180005385.01.T01:CDS
AATCACCAATGGAAACAGTG+GGG	0.793032	1:+91128344	None:intergenic
AATCTTCTCCTTCATTTCCA+TGG	0.462730	1:-91127834	MsG0180005385.01.T01:CDS
AATGGGGAGAAAATGGGGTA+GGG	0.605614	1:-91127075	MsG0180005385.01.T01:three_prime_UTR
ACCAAATAAATGTGGTGAAA+TGG	0.288690	1:-91127045	MsG0180005385.01.T01:three_prime_UTR
ACTACTCCAGGTCCTTCTTC+AGG	0.382607	1:-91127868	MsG0180005385.01.T01:CDS
ACTCCAGGTCCTTCTTCAGG+TGG	0.643588	1:-91127865	MsG0180005385.01.T01:CDS
ACTGTATAGGAGGTAGTGCT+AGG	0.580442	1:+91128134	None:intergenic
AGATCCATCAACAAGAATTG+TGG	0.619785	1:+91128381	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCAAATAAATGTGGTGAAA+TGG	-	Chr1:91128426-91128445	MsG0180005385.01.T01:CDS	30.0%
AGAACCAAGATGAAATTTGA+AGG	+	Chr1:91127368-91127387	None:intergenic	30.0%
ATGGTGATAATACTTACTGA+TGG	+	Chr1:91127153-91127172	None:intergenic	30.0%
CCAAATAAATGTGGTGAAAT+GGG	-	Chr1:91128427-91128446	MsG0180005385.01.T01:CDS	30.0%
CTACAAAACCTCTTCTTATT+TGG	-	Chr1:91126956-91126975	MsG0180005385.01.T01:three_prime_UTR	30.0%
CTGTAAGAAGAAAGAATTGT+AGG	-	Chr1:91128359-91128378	MsG0180005385.01.T01:CDS	30.0%
GAACCAAGATGAAATTTGAA+GGG	+	Chr1:91127367-91127386	None:intergenic	30.0%
GAGATTGATGAATTACTGAT+TGG	-	Chr1:91128251-91128270	MsG0180005385.01.T01:intron	30.0%
GATGGTAAACCAAATAAATG+TGG	-	Chr1:91128418-91128437	MsG0180005385.01.T01:CDS	30.0%
GTTATGTGAGTATTTATCTC+TGG	+	Chr1:91126929-91126948	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	91126917	91128576	91126917	ID=MsG0180005385.01;Name=MsG0180005385.01
Chr1	mRNA	91126917	91128576	91126917	ID=MsG0180005385.01.T01;Parent=MsG0180005385.01;Name=MsG0180005385.01.T01;_AED=0.44;_eAED=0.44;_QI=109\|0.33\|0.75\|1\|0.33\|0.5\|4\|212\|205
Chr1	exon	91126917	91127190	91126917	ID=MsG0180005385.01.T01:exon:48195;Parent=MsG0180005385.01.T01
Chr1	exon	91127644	91127953	91127644	ID=MsG0180005385.01.T01:exon:48194;Parent=MsG0180005385.01.T01
Chr1	exon	91128136	91128251	91128136	ID=MsG0180005385.01.T01:exon:48193;Parent=MsG0180005385.01.T01
Chr1	exon	91128338	91128576	91128338	ID=MsG0180005385.01.T01:exon:48192;Parent=MsG0180005385.01.T01
Chr1	five_prime_UTR	91128468	91128576	91128468	ID=MsG0180005385.01.T01:five_prime_utr;Parent=MsG0180005385.01.T01
Chr1	CDS	91128338	91128467	91128338	ID=MsG0180005385.01.T01:cds;Parent=MsG0180005385.01.T01
Chr1	CDS	91128136	91128251	91128136	ID=MsG0180005385.01.T01:cds;Parent=MsG0180005385.01.T01
Chr1	CDS	91127644	91127953	91127644	ID=MsG0180005385.01.T01:cds;Parent=MsG0180005385.01.T01
Chr1	CDS	91127129	91127190	91127129	ID=MsG0180005385.01.T01:cds;Parent=MsG0180005385.01.T01
Chr1	three_prime_UTR	91126917	91127128	91126917	ID=MsG0180005385.01.T01:three_prime_utr;Parent=MsG0180005385.01.T01

Gene Sequence

>MsG0180005385.01.T01

ATGTCTCTTCCTATTTTCTTTTACTTTCTTATACTTTCACTGTTCTTCAAGCTTTCACACTCCACCACAATTCTTGTTGATGGATCTTCAGAGTGGAAGAACCCCACTGTTTCCATTGGTGATTCCATCACTTTCAAGCACAAACAAAACTACAACCTCTACATTTTCAAGAACCAGAAAGCCTTCAATCTCTGCAATTTCACTCAAGCCAATCTTCTCACTGATCCTAGCACTACCTCCTATACAAAGCTAGCCATAAAGGTAACTCCAACAAAAGCTTCAGCACCAGAAGCTTCTTCACCAATGCCAACTACTCCAGGTCCTTCTTCAGGTGGAGATATACAATCTTCTCCTTCATTTCCATGGCCATTTCGTCCTCACCAAGGTTCTTCACCAGGTCCAGCACCAACACCAGAAGCAAGTTCACCAATAACACTTCCATTAGTACCATACAAAGGTAGTGGTGATGGCATGCCTTTCATTAACAGTAACCCTGCAGTTCCTCTACCTACTGGTGAAGTTGATTCTGCTACTATACACCCCCTTGCTACTTCTGGTGATGATTGGATCATTGGATTTCATGCTGCTGTGCACATTATGGCTTTACTGCTGCTGTAA

Protein sequence

>MsG0180005385.01.T01

MSLPIFFYFLILSLFFKLSHSTTILVDGSSEWKNPTVSIGDSITFKHKQNYNLYIFKNQKAFNLCNFTQANLLTDPSTTSYTKLAIKVTPTKASAPEASSPMPTTPGPSSGGDIQSSPSFPWPFRPHQGSSPGPAPTPEASSPITLPLVPYKGSGDGMPFINSNPAVPLPTGEVDSATIHPLATSGDDWIIGFHAAVHIMALLLL*