AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005586.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005586.01.T01 MTR_1g103290 99.306 144 1 0 1 144 1 144 4.45e-103 298
MsG0180005586.01.T01 MTR_7g095280 68.027 147 44 2 1 144 5 151 2.97e-66 204
MsG0180005586.01.T01 MTR_1g103290 97.938 97 2 0 48 144 24 120 1.36e-61 191
MsG0180005586.01.T01 MTR_5g056640 54.545 143 58 1 1 143 8 143 2.52e-51 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005586.01.T01 AT3G22740 72.222 144 40 0 1 144 12 155 7.72e-75 227
MsG0180005586.01.T01 AT3G63250 70.139 144 39 2 2 144 9 149 9.33e-70 213
MsG0180005586.01.T01 AT3G63250 70.139 144 39 2 2 144 9 149 9.33e-70 213
MsG0180005586.01.T01 AT3G25900 53.472 144 60 1 1 144 9 145 2.80e-52 167
MsG0180005586.01.T01 AT3G25900 53.472 144 60 1 1 144 9 145 1.51e-51 166
MsG0180005586.01.T01 AT3G25900 53.472 144 60 1 1 144 9 145 2.58e-51 166
MsG0180005586.01.T01 AT3G63250 63.551 107 32 3 39 144 9 109 5.11e-39 133

Find 85 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCGAAGACAAATGCATTT+TGG 0.209442 1:-93645576 None:intergenic
AACAGAAGCTGCAATTAAAA+TGG 0.215421 1:-93645346 None:intergenic
TTTGATTTCATCTATTAAAA+AGG 0.245381 1:+93646051 MsG0180005586.01.T01:three_prime_UTR
GTTAGCTTTATGTCTTGTTT+TGG 0.254704 1:+93647028 MsG0180005586.01.T01:three_prime_UTR
ATAGGCTTATGCTGAACTTT+TGG 0.263229 1:+93645865 MsG0180005586.01.T01:intron
CTTCGAGTATGATGAAATTA+AGG 0.317586 1:+93645597 MsG0180005586.01.T01:three_prime_UTR
CACATTCTAATATATAGTTT+TGG 0.324623 1:-93645433 None:intergenic
AATATTAACATCATCTTATC+AGG 0.326619 1:+93645076 MsG0180005586.01.T01:CDS
ACTTGTGCATCAAGCTTGTT+TGG 0.326834 1:-93645760 None:intergenic
ATTAGAAATTAAAGATTGTT+TGG 0.327861 1:-93647125 None:intergenic
TTCAGAGCCATCAGCTAAAT+AGG 0.334984 1:-93645382 None:intergenic
GGCATAACCGAGGCATTATA+TGG 0.343231 1:+93646893 MsG0180005586.01.T01:three_prime_UTR
TCCTACAACAGCCACCAAAA+AGG 0.354500 1:-93646850 None:intergenic
ATGTGTGTAATAATTAATAA+TGG 0.370895 1:+93646974 MsG0180005586.01.T01:three_prime_UTR
TGTTGATTGATGTTGAAGTA+TGG 0.373794 1:+93646943 MsG0180005586.01.T01:three_prime_UTR
ATATGGGTAGCCATACAAAT+TGG 0.386005 1:+93647095 MsG0180005586.01.T01:three_prime_UTR
TGTGAAAATCTTTGAGTGTC+TGG 0.387154 1:-93645678 None:intergenic
TGGCATATATCCAATTTGTA+TGG 0.403209 1:-93647105 None:intergenic
ATCAAACCATGGATGAATCT+AGG 0.415611 1:-93646034 None:intergenic
TGAAGCTAAAGGGTTCTCTA+AGG 0.426974 1:+93645200 MsG0180005586.01.T01:CDS
TCACAATAATGTAAAGGAAA+TGG 0.427228 1:+93647006 MsG0180005586.01.T01:three_prime_UTR
TATATTCAGGCCACAATTCA+AGG 0.428301 1:+93645174 MsG0180005586.01.T01:intron
CTGTTGGGATATACAAGTAT+TGG 0.438211 1:-93646311 None:intergenic
ATTATAAGTCTCTCCACTGT+TGG 0.440104 1:-93646327 None:intergenic
TAACGCCACTTTCCTATGTA+AGG 0.444879 1:-93646815 None:intergenic
TCATAACACAAGAAAACATT+AGG 0.444929 1:+93642718 None:intergenic
AAGTTTGCACAAACAGAAAC+AGG 0.449989 1:-93647174 None:intergenic
CTTGTTTGGAATTGTTTCAA+AGG 0.456143 1:-93645746 None:intergenic
GCAGCTTCTGTTGGAAGCTA+TGG 0.473802 1:+93645357 MsG0180005586.01.T01:CDS
CTGAACAAGTGTGGTGGGTA+TGG 0.473956 1:+93642770 MsG0180005586.01.T01:CDS
TTATAAGTCTCTCCACTGTT+GGG 0.479869 1:-93646326 None:intergenic
TGGAGAAGTAAAGAGGCATT+TGG 0.480677 1:-93642864 None:intergenic
TGTTCGCAAGTTGTCGCAGT+TGG 0.481768 1:+93645995 MsG0180005586.01.T01:three_prime_UTR
TAGGAAAGTGGCGTTACGCC+GGG 0.482381 1:+93646822 MsG0180005586.01.T01:three_prime_UTR
GGTGGGTATGGAATCATAGA+TGG 0.482779 1:+93642782 MsG0180005586.01.T01:CDS
ACAGAAGCTGCAATTAAAAT+GGG 0.497453 1:-93645345 None:intergenic
TAGACCTCAACGACCCACTT+TGG 0.510920 1:+93642837 MsG0180005586.01.T01:CDS
TTTAGAATTTCAACAAATTG+TGG 0.512948 1:+93645520 MsG0180005586.01.T01:three_prime_UTR
CTCCTGAGTCAAGGTAATCA+AGG 0.520965 1:-93645046 None:intergenic
CACCTTGATTACCTTGACTC+AGG 0.525558 1:+93645044 MsG0180005586.01.T01:CDS
CTTCTCCACATCTTGTCCGA+AGG 0.527108 1:+93642879 MsG0180005586.01.T01:CDS
TTTCATCATGTTGAGCTATG+TGG 0.528363 1:-93642741 None:intergenic
GCATTATATGGAAAACCTCA+TGG 0.530944 1:+93646905 MsG0180005586.01.T01:three_prime_UTR
AAATGATCACAATAATGTAA+AGG 0.535266 1:+93647000 MsG0180005586.01.T01:three_prime_UTR
CACTCCAAAGTGGGTCGTTG+AGG 0.542769 1:-93642841 None:intergenic
AATTTATGAAGAAATCAAGT+GGG 0.544226 1:+93646771 MsG0180005586.01.T01:intron
TTGTCCCTTACATAGGAAAG+TGG 0.553912 1:+93646810 MsG0180005586.01.T01:three_prime_UTR
GAAATCAAGTGGGGAAGCAG+AGG 0.557172 1:+93646781 MsG0180005586.01.T01:intron
ATAGGAAAGTGGCGTTACGC+CGG 0.558321 1:+93646821 MsG0180005586.01.T01:three_prime_UTR
GCCACGGAGCTTGAACGCCA+TGG 0.563345 1:+93642812 MsG0180005586.01.T01:CDS
AAAGGGTTCTCTAAGGAAGA+AGG 0.563843 1:+93645207 MsG0180005586.01.T01:CDS
TGTACCCTTCGGACAAGATG+TGG 0.570045 1:-93642884 None:intergenic
AGCTTCAAAACCTTGAATTG+TGG 0.571705 1:-93645184 None:intergenic
AAGCGTAGAGCTTGCACGCG+AGG 0.576030 1:+93645248 MsG0180005586.01.T01:CDS
TTCTCCACATCTTGTCCGAA+GGG 0.579675 1:+93642880 MsG0180005586.01.T01:CDS
TATGGTGCCTATTTAGCTGA+TGG 0.580561 1:+93645375 MsG0180005586.01.T01:CDS
GTGCATGTCATGTATGAAAG+TGG 0.583458 1:-93647056 None:intergenic
CAAGATGTGGAGAAGTAAAG+AGG 0.590997 1:-93642871 None:intergenic
GGTCGTTGAGGTCTACTCCA+TGG 0.592047 1:-93642829 None:intergenic
ACTGCTCCTAGATTCATCCA+TGG 0.596721 1:+93646028 MsG0180005586.01.T01:three_prime_UTR
GGCATTTGGCACTCCAAAGT+GGG 0.599825 1:-93642850 None:intergenic
CACGTGATATATATTATGAT+AGG 0.600456 1:+93645271 MsG0180005586.01.T01:CDS
ATAATGCTGACAACAACACA+TGG 0.602437 1:+93646345 MsG0180005586.01.T01:three_prime_UTR
CATAGATGGTGGGTTTGCCA+CGG 0.604757 1:+93642796 MsG0180005586.01.T01:CDS
TTGCGAACATGAATCAGCAA+TGG 0.608422 1:-93645981 None:intergenic
TTATGTTAGCTCCTGAGTCA+AGG 0.611515 1:-93645055 None:intergenic
TGCACAAACAGAAACAGGTA+AGG 0.611945 1:-93647169 None:intergenic
TAATTTATGAAGAAATCAAG+TGG 0.612573 1:+93646770 MsG0180005586.01.T01:intron
ATATTATGATAGGTGCACTA+AGG 0.613444 1:+93645281 MsG0180005586.01.T01:CDS
TAATAGATGAAATCAAACCA+TGG 0.620768 1:-93646045 None:intergenic
GGGTATGGAATCATAGATGG+TGG 0.626556 1:+93642785 MsG0180005586.01.T01:CDS
ATTTATGAAGAAATCAAGTG+GGG 0.627728 1:+93646772 MsG0180005586.01.T01:intron
TTGCAAGGATGAAAACAAAG+TGG 0.632290 1:+93645928 MsG0180005586.01.T01:three_prime_UTR
AGGCATTTGGCACTCCAAAG+TGG 0.633919 1:-93642851 None:intergenic
AAGCTGCAATTAAAATGGGA+CGG 0.635149 1:-93645341 None:intergenic
AACGCCACTTTCCTATGTAA+GGG 0.636973 1:-93646814 None:intergenic
GATGAAAACAAAGTGGCTAG+TGG 0.639603 1:+93645935 MsG0180005586.01.T01:three_prime_UTR
AAGGAATAGAAATTCCTGCA+TGG 0.641430 1:+93645894 MsG0180005586.01.T01:three_prime_UTR
GGTATGGAATCATAGATGGT+GGG 0.641589 1:+93642786 MsG0180005586.01.T01:CDS
TCCATGGCGTTCAAGCTCCG+TGG 0.672736 1:-93642813 None:intergenic
AGGAAAGTGGCGTTACGCCG+GGG 0.681756 1:+93646823 MsG0180005586.01.T01:three_prime_UTR
AACTATTAGAGGCATAACCG+AGG 0.688332 1:+93646883 MsG0180005586.01.T01:three_prime_UTR
TAATGCTGACAACAACACAT+GGG 0.709280 1:+93646346 MsG0180005586.01.T01:three_prime_UTR
ATACTTGTATATCCCAACAG+TGG 0.714658 1:+93646314 MsG0180005586.01.T01:three_prime_UTR
TGCTGACAACAACACATGGG+TGG 0.718263 1:+93646349 MsG0180005586.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAATTTAAAATTTTTGT+TGG - Chr1:93643233-93643252 None:intergenic 10.0%
!!! GAAAATTTAAAATTTTTGTT+GGG - Chr1:93643232-93643251 None:intergenic 10.0%
!!! TTATATTTTATATCGAAAAA+AGG + Chr1:93643465-93643484 MsG0180005586.01.T01:intron 10.0%
!! AAATTATCCTTATTAACTAT+TGG - Chr1:93644599-93644618 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + Chr1:93644598-93644617 MsG0180005586.01.T01:intron 15.0%
!! AGTTGATTAAATTATATGTA+GGG - Chr1:93645125-93645144 None:intergenic 15.0%
!! ATAAAGATTAAAATAAGACA+AGG + Chr1:93644072-93644091 MsG0180005586.01.T01:intron 15.0%
!! ATGTGTGTAATAATTAATAA+TGG + Chr1:93646974-93646993 MsG0180005586.01.T01:three_prime_UTR 15.0%
!! ATTAGAAATTAAAGATTGTT+TGG - Chr1:93647128-93647147 None:intergenic 15.0%
!! CAAAATTTATCTTCAATAAT+CGG + Chr1:93646483-93646502 MsG0180005586.01.T01:intron 15.0%
!! TCAGTTAATTATATAAATGT+TGG - Chr1:93644377-93644396 None:intergenic 15.0%
!! TTAAACAAAAGATAAATGTT+AGG - Chr1:93643442-93643461 None:intergenic 15.0%
!!! ATATTAAGATTCATGTTTTT+AGG - Chr1:93646518-93646537 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - Chr1:93644575-93644594 None:intergenic 15.0%
!!! TATACTTTAAAGAATTTCAA+TGG + Chr1:93644273-93644292 MsG0180005586.01.T01:intron 15.0%
!!! TTTGATTTCATCTATTAAAA+AGG + Chr1:93646051-93646070 MsG0180005586.01.T01:three_prime_UTR 15.0%
!! AAACTAACCAATAGTTAATA+AGG + Chr1:93644589-93644608 MsG0180005586.01.T01:intron 20.0%
!! AAATGATCACAATAATGTAA+AGG + Chr1:93647000-93647019 MsG0180005586.01.T01:three_prime_UTR 20.0%
!! AAGGAAAAAATGTATAACTT+AGG + Chr1:93643770-93643789 MsG0180005586.01.T01:intron 20.0%
!! AATATTAACATCATCTTATC+AGG + Chr1:93645076-93645095 MsG0180005586.01.T01:CDS 20.0%
!! AATTTATGAAGAAATCAAGT+GGG + Chr1:93646771-93646790 MsG0180005586.01.T01:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - Chr1:93644512-93644531 None:intergenic 20.0%
!! ATTCAAATCATATAGTTTCT+TGG + Chr1:93646182-93646201 MsG0180005586.01.T01:intron 20.0%
!! ATTCACATTATTAATGATCT+TGG - Chr1:93643105-93643124 None:intergenic 20.0%
!! ATTTAATGATGATTTATCGA+AGG + Chr1:93646635-93646654 MsG0180005586.01.T01:intron 20.0%
!! CAGTTGATTAAATTATATGT+AGG - Chr1:93645126-93645145 None:intergenic 20.0%
!! GTTCTATATGTTAAAAACTT+AGG - Chr1:93643144-93643163 None:intergenic 20.0%
!! TAAAGACATTTGATATGTTA+GGG + Chr1:93644838-93644857 MsG0180005586.01.T01:intron 20.0%
!! TAATTTATGAAGAAATCAAG+TGG + Chr1:93646770-93646789 MsG0180005586.01.T01:intron 20.0%
!! TGAGATTAATAACTTAGAAA+TGG - Chr1:93646465-93646484 None:intergenic 20.0%
!! TGATAAGTTACAAGATAAAT+TGG + Chr1:93644952-93644971 MsG0180005586.01.T01:intron 20.0%
!! TTTCATACACTAAACAAAAA+TGG - Chr1:93646735-93646754 None:intergenic 20.0%
!!! AATTTTGTGTTATTATTGCT+AGG + Chr1:93644399-93644418 MsG0180005586.01.T01:intron 20.0%
!!! ATTAGAATGTGATTTTAGAT+TGG + Chr1:93645445-93645464 MsG0180005586.01.T01:three_prime_UTR 20.0%
!!! CACATTCTAATATATAGTTT+TGG - Chr1:93645436-93645455 None:intergenic 20.0%
!!! CTTTTGAAACTAAAAAGAAA+AGG + Chr1:93643751-93643770 MsG0180005586.01.T01:intron 20.0%
!!! GTTTTTGTTTCAATTCATAT+AGG + Chr1:93642931-93642950 MsG0180005586.01.T01:intron 20.0%
!!! TTCAAAAGTATATCAAAATC+CGG - Chr1:93643739-93643758 None:intergenic 20.0%
!!! TTTAGAATTTCAACAAATTG+TGG + Chr1:93645520-93645539 MsG0180005586.01.T01:three_prime_UTR 20.0%
! AAAATAACCAAAATGCTCTA+AGG + Chr1:93644203-93644222 MsG0180005586.01.T01:intron 25.0%
! AAACTTGTACAAATTTGCAA+AGG - Chr1:93645798-93645817 None:intergenic 25.0%
! AAATAACCAAAATGCTCTAA+GGG + Chr1:93644204-93644223 MsG0180005586.01.T01:intron 25.0%
! AAGAATCACTATATACAAGA+TGG + Chr1:93643018-93643037 MsG0180005586.01.T01:intron 25.0%
! AAGCCTATATATTTGATTGT+AGG - Chr1:93645853-93645872 None:intergenic 25.0%
! AATAACTTAGAAATGGAATG+AGG - Chr1:93646458-93646477 None:intergenic 25.0%
! AATGATTAAGAGAAAGAGAA+TGG - Chr1:93644632-93644651 None:intergenic 25.0%
! AGCCTATATATTTGATTGTA+GGG - Chr1:93645852-93645871 None:intergenic 25.0%
! ATTTATGAAGAAATCAAGTG+GGG + Chr1:93646772-93646791 MsG0180005586.01.T01:intron 25.0%
! CACGTGATATATATTATGAT+AGG + Chr1:93645271-93645290 MsG0180005586.01.T01:CDS 25.0%
! CATATAGAACAAACTTCAAT+TGG + Chr1:93643154-93643173 MsG0180005586.01.T01:intron 25.0%
! CTATCTATCTATCTATATTC+AGG + Chr1:93645161-93645180 MsG0180005586.01.T01:intron 25.0%
! GCAATAAATCAACAAAACAT+CGG - Chr1:93645004-93645023 None:intergenic 25.0%
! GTAAAGACATTTGATATGTT+AGG + Chr1:93644837-93644856 MsG0180005586.01.T01:intron 25.0%
! TAAGATATGAAAGAATTCTC+CGG + Chr1:93643717-93643736 MsG0180005586.01.T01:intron 25.0%
! TAATAGATGAAATCAAACCA+TGG - Chr1:93646048-93646067 None:intergenic 25.0%
! TACAAATTTGCAAAGGTAAT+TGG - Chr1:93645791-93645810 None:intergenic 25.0%
! TCACAATAATGTAAAGGAAA+TGG + Chr1:93647006-93647025 MsG0180005586.01.T01:three_prime_UTR 25.0%
! TCCTTGAATTCTTAAATATC+AGG + Chr1:93646277-93646296 MsG0180005586.01.T01:intron 25.0%
! TGATTGTAGGGAAAATTAAT+AGG - Chr1:93645840-93645859 None:intergenic 25.0%
! TTCCCTACAATCAAATATAT+AGG + Chr1:93645847-93645866 MsG0180005586.01.T01:intron 25.0%
! TTGGTATTTGTACAAGAAAA+GGG + Chr1:93643922-93643941 MsG0180005586.01.T01:intron 25.0%
! TTTGAACTTACATCATACTT+TGG - Chr1:93644171-93644190 None:intergenic 25.0%
! TTTGGTATTTGTACAAGAAA+AGG + Chr1:93643921-93643940 MsG0180005586.01.T01:intron 25.0%
!! GTTTATATACATTTTCCATG+AGG - Chr1:93646923-93646942 None:intergenic 25.0%
!! TCATATCTTAAAGCTTTTGA+TGG - Chr1:93643707-93643726 None:intergenic 25.0%
!! TTAATTGCATGTATTTGCTA+CGG - Chr1:93643303-93643322 None:intergenic 25.0%
!! TTGGATCTTTTGTTAGTAAA+CGG + Chr1:93643173-93643192 MsG0180005586.01.T01:intron 25.0%
!!! AAAATCTATTAAGCGTTGAA+AGG - Chr1:93644884-93644903 None:intergenic 25.0%
!!! ATTGTGTGTTTTTTGATAGA+GGG + Chr1:93645632-93645651 MsG0180005586.01.T01:three_prime_UTR 25.0%
!!! TACATCATACTTTGGTTTTT+TGG - Chr1:93644163-93644182 None:intergenic 25.0%
!!! TCATACAAGCATAATTTGTT+TGG + Chr1:93644046-93644065 MsG0180005586.01.T01:intron 25.0%
!!! TCATTTTTGCACAAAGATAT+GGG + Chr1:93647079-93647098 MsG0180005586.01.T01:three_prime_UTR 25.0%
!!! TCTAATATATAGTTTTGGCA+TGG - Chr1:93645431-93645450 None:intergenic 25.0%
AACAGAAGCTGCAATTAAAA+TGG - Chr1:93645349-93645368 None:intergenic 30.0%
AACTGCTATCAGATTGAAAT+CGG - Chr1:93646418-93646437 None:intergenic 30.0%
ACAGAAGCTGCAATTAAAAT+GGG - Chr1:93645348-93645367 None:intergenic 30.0%
ATATTATGATAGGTGCACTA+AGG + Chr1:93645281-93645300 MsG0180005586.01.T01:CDS 30.0%
CTAGGTGTAATGTCTTAATA+AGG + Chr1:93643574-93643593 MsG0180005586.01.T01:intron 30.0%
CTTCGAGTATGATGAAATTA+AGG + Chr1:93645597-93645616 MsG0180005586.01.T01:three_prime_UTR 30.0%
GCCTGATATTTAAGAATTCA+AGG - Chr1:93646281-93646300 None:intergenic 30.0%
GTAATGTCTTAATAAGGTTC+AGG + Chr1:93643580-93643599 MsG0180005586.01.T01:intron 30.0%
TAATGATGATTTATCGAAGG+TGG + Chr1:93646638-93646657 MsG0180005586.01.T01:intron 30.0%
TGAAAACAACTTAAAGCAGA+CGG + Chr1:93643871-93643890 MsG0180005586.01.T01:intron 30.0%
TGGCATATATCCAATTTGTA+TGG - Chr1:93647108-93647127 None:intergenic 30.0%
! AAAGATTTTCTGAACAAGTG+TGG + Chr1:93642761-93642780 MsG0180005586.01.T01:CDS 30.0%
! ATGGTTTTCTTTTTCTTGCA+AGG + Chr1:93645913-93645932 MsG0180005586.01.T01:three_prime_UTR 30.0%
! CTCAATCATCAATTTGAGAT+CGG - Chr1:93646676-93646695 None:intergenic 30.0%
! CTTGTTTGGAATTGTTTCAA+AGG - Chr1:93645749-93645768 None:intergenic 30.0%
! GTTATGCCTCTAATAGTTTT+TGG - Chr1:93646881-93646900 None:intergenic 30.0%
! TAATTGCATGTATTTGCTAC+GGG - Chr1:93643302-93643321 None:intergenic 30.0%
! TGTTGATTGATGTTGAAGTA+TGG + Chr1:93646943-93646962 MsG0180005586.01.T01:three_prime_UTR 30.0%
!! AAACGACACTCATTTTGAAA+CGG + Chr1:93644681-93644700 MsG0180005586.01.T01:intron 30.0%
!! ATATCTCTAAAACACAGTCA+AGG + Chr1:93642979-93642998 MsG0180005586.01.T01:intron 30.0%
!! CTCATTTTTGCACAAAGATA+TGG + Chr1:93647078-93647097 MsG0180005586.01.T01:three_prime_UTR 30.0%
!! TACAAGCATAATTTGTTTGG+AGG + Chr1:93644049-93644068 MsG0180005586.01.T01:intron 30.0%
!!! ATTCAAGGTTTTGAAGCTAA+AGG + Chr1:93645189-93645208 MsG0180005586.01.T01:CDS 30.0%
!!! ATTTTAATTGCAGCTTCTGT+TGG + Chr1:93645348-93645367 MsG0180005586.01.T01:CDS 30.0%
!!! GATTGTGTGTTTTTTGATAG+AGG + Chr1:93645631-93645650 MsG0180005586.01.T01:three_prime_UTR 30.0%
!!! GGGAAAGATTTTAAGTTCAT+TGG + Chr1:93644224-93644243 MsG0180005586.01.T01:intron 30.0%
!!! GTTAGCTTTATGTCTTGTTT+TGG + Chr1:93647028-93647047 MsG0180005586.01.T01:three_prime_UTR 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - Chr1:93644680-93644699 None:intergenic 30.0%
!!! TTCAAGGTTTTGAAGCTAAA+GGG + Chr1:93645190-93645209 MsG0180005586.01.T01:CDS 30.0%
!!! TTGTGTGTTTTTTGATAGAG+GGG + Chr1:93645633-93645652 MsG0180005586.01.T01:three_prime_UTR 30.0%
AAAAGAGAAGTTGTACCCTT+CGG - Chr1:93642898-93642917 None:intergenic 35.0%
AACATGTACATATCAGACGT+GGG + Chr1:93646553-93646572 MsG0180005586.01.T01:intron 35.0%
AAGACAAGGCTACTAAAACA+AGG + Chr1:93644086-93644105 MsG0180005586.01.T01:intron 35.0%
AAGCTGCAATTAAAATGGGA+CGG - Chr1:93645344-93645363 None:intergenic 35.0%
AAGGAATAGAAATTCCTGCA+TGG + Chr1:93645894-93645913 MsG0180005586.01.T01:three_prime_UTR 35.0%
AAGGTTCAGGAGAAAGTTTA+TGG + Chr1:93643593-93643612 MsG0180005586.01.T01:intron 35.0%
ACAAGAGATAAAACACAGTC+AGG + Chr1:93643046-93643065 MsG0180005586.01.T01:intron 35.0%
ACATTTGATATGTTAGGGTG+CGG + Chr1:93644843-93644862 MsG0180005586.01.T01:intron 35.0%
ACCACTCCAAAAACTATTAG+AGG + Chr1:93646872-93646891 MsG0180005586.01.T01:three_prime_UTR 35.0%
AGACAAGGCTACTAAAACAA+GGG + Chr1:93644087-93644106 MsG0180005586.01.T01:intron 35.0%
AGCTTCAAAACCTTGAATTG+TGG - Chr1:93645187-93645206 None:intergenic 35.0%
AGTTACTTACTTTAGCTGTC+TGG + Chr1:93646591-93646610 MsG0180005586.01.T01:intron 35.0%
ATAATGCTGACAACAACACA+TGG + Chr1:93646345-93646364 MsG0180005586.01.T01:three_prime_UTR 35.0%
ATACTTGTATATCCCAACAG+TGG + Chr1:93646314-93646333 MsG0180005586.01.T01:three_prime_UTR 35.0%
ATATGGGTAGCCATACAAAT+TGG + Chr1:93647095-93647114 MsG0180005586.01.T01:three_prime_UTR 35.0%
ATCAAACCATGGATGAATCT+AGG - Chr1:93646037-93646056 None:intergenic 35.0%
ATTATAAGTCTCTCCACTGT+TGG - Chr1:93646330-93646349 None:intergenic 35.0%
CAAAAACACGTAGACACTAA+TGG - Chr1:93645487-93645506 None:intergenic 35.0%
CAAGAAAAAGAAAACCATGC+AGG - Chr1:93645911-93645930 None:intergenic 35.0%
CAAGAGATAAAACACAGTCA+GGG + Chr1:93643047-93643066 MsG0180005586.01.T01:intron 35.0%
CAAGTAACGAAATGTACAAG+TGG + Chr1:93643496-93643515 MsG0180005586.01.T01:intron 35.0%
CTGTTGGGATATACAAGTAT+TGG - Chr1:93646314-93646333 None:intergenic 35.0%
GCATTATATGGAAAACCTCA+TGG + Chr1:93646905-93646924 MsG0180005586.01.T01:three_prime_UTR 35.0%
GTAAAAATTCTTGTTGACGC+AGG + Chr1:93645709-93645728 MsG0180005586.01.T01:three_prime_UTR 35.0%
GTTGAAAGGTGAAAAGTTGA+GGG - Chr1:93644870-93644889 None:intergenic 35.0%
TAAAGTAAGTAACTGCGTCT+AGG - Chr1:93646585-93646604 None:intergenic 35.0%
TAATGCTGACAACAACACAT+GGG + Chr1:93646346-93646365 MsG0180005586.01.T01:three_prime_UTR 35.0%
TACAAGCTGTAGTGTTCATT+TGG + Chr1:93643903-93643922 MsG0180005586.01.T01:intron 35.0%
TATATTCAGGCCACAATTCA+AGG + Chr1:93645174-93645193 MsG0180005586.01.T01:intron 35.0%
TGTGAAAATCTTTGAGTGTC+TGG - Chr1:93645681-93645700 None:intergenic 35.0%
TTAGATGAGATTGACGTGTT+TGG + Chr1:93643807-93643826 MsG0180005586.01.T01:intron 35.0%
TTATAAGTCTCTCCACTGTT+GGG - Chr1:93646329-93646348 None:intergenic 35.0%
TTGCAAGGATGAAAACAAAG+TGG + Chr1:93645928-93645947 MsG0180005586.01.T01:three_prime_UTR 35.0%
TTTCATCATGTTGAGCTATG+TGG - Chr1:93642744-93642763 None:intergenic 35.0%
TTTGTTAGTAAACGGTGGAT+TGG + Chr1:93643181-93643200 MsG0180005586.01.T01:intron 35.0%
! AGACACTCAAAGATTTTCAC+AGG + Chr1:93645680-93645699 MsG0180005586.01.T01:three_prime_UTR 35.0%
! AGGTTTTCCATATAATGCCT+CGG - Chr1:93646903-93646922 None:intergenic 35.0%
! AGTCGAAGACAAATGCATTT+TGG - Chr1:93645579-93645598 None:intergenic 35.0%
! ATAGGCTTATGCTGAACTTT+TGG + Chr1:93645865-93645884 MsG0180005586.01.T01:intron 35.0%
! ATGTGCTTGCTAAATGTTTG+TGG - Chr1:93643077-93643096 None:intergenic 35.0%
! ATTTTCTGAACAAGTGTGGT+GGG + Chr1:93642765-93642784 MsG0180005586.01.T01:CDS 35.0%
! CAAAGATTTTCACAGGGAAA+GGG + Chr1:93645687-93645706 MsG0180005586.01.T01:three_prime_UTR 35.0%
! CATTAGTGTCTACGTGTTTT+TGG + Chr1:93645485-93645504 MsG0180005586.01.T01:three_prime_UTR 35.0%
! CGTCAATCTCATCTAAGATA+AGG - Chr1:93643803-93643822 None:intergenic 35.0%
! GAAGATTTTGTCCCTTACAT+AGG + Chr1:93646803-93646822 MsG0180005586.01.T01:three_prime_UTR 35.0%
! GACACTCAAAGATTTTCACA+GGG + Chr1:93645681-93645700 MsG0180005586.01.T01:three_prime_UTR 35.0%
! GATCTTTTGTTAGTAAACGG+TGG + Chr1:93643176-93643195 MsG0180005586.01.T01:intron 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - Chr1:93644550-93644569 None:intergenic 35.0%
! TCAAAGATTTTCACAGGGAA+AGG + Chr1:93645686-93645705 MsG0180005586.01.T01:three_prime_UTR 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - Chr1:93644546-93644565 None:intergenic 35.0%
!! ATCTTTCCCTTAGAGCATTT+TGG - Chr1:93644213-93644232 None:intergenic 35.0%
!!! TTTTGTTCTAAAGAGCGTAG+TGG - Chr1:93644914-93644933 None:intergenic 35.0%
!!! TTTTTTGATAGAGGGGACTA+TGG + Chr1:93645640-93645659 MsG0180005586.01.T01:three_prime_UTR 35.0%
AAGCGACACTCATTTCGAAA+CGG - Chr1:93644446-93644465 None:intergenic 40.0%
AGTGGCACTTTATGTTGACA+AGG + Chr1:93643514-93643533 MsG0180005586.01.T01:intron 40.0%
ATCAAGGTGAACCTGTTACA+TGG - Chr1:93645033-93645052 None:intergenic 40.0%
ATTGCTTGACACCATGTAAC+AGG + Chr1:93645019-93645038 MsG0180005586.01.T01:intron 40.0%
CAACATGTACATATCAGACG+TGG + Chr1:93646552-93646571 MsG0180005586.01.T01:intron 40.0%
CAAGATGTGGAGAAGTAAAG+AGG - Chr1:93642874-93642893 None:intergenic 40.0%
CAGTACTCAGTACTTCATCA+TGG + Chr1:93643540-93643559 MsG0180005586.01.T01:intron 40.0%
CATTTGATATGTTAGGGTGC+GGG + Chr1:93644844-93644863 MsG0180005586.01.T01:intron 40.0%
CGTTGAAAGGTGAAAAGTTG+AGG - Chr1:93644871-93644890 None:intergenic 40.0%
GATGAAAACAAAGTGGCTAG+TGG + Chr1:93645935-93645954 MsG0180005586.01.T01:three_prime_UTR 40.0%
GGTATGGAATCATAGATGGT+GGG + Chr1:93642786-93642805 MsG0180005586.01.T01:CDS 40.0%
GTGCATGTCATGTATGAAAG+TGG - Chr1:93647059-93647078 None:intergenic 40.0%
TATGGTGCCTATTTAGCTGA+TGG + Chr1:93645375-93645394 MsG0180005586.01.T01:CDS 40.0%
TGAAGCTAAAGGGTTCTCTA+AGG + Chr1:93645200-93645219 MsG0180005586.01.T01:CDS 40.0%
TTATGTTAGCTCCTGAGTCA+AGG - Chr1:93645058-93645077 None:intergenic 40.0%
TTCAGAGCCATCAGCTAAAT+AGG - Chr1:93645385-93645404 None:intergenic 40.0%
TTGCGAACATGAATCAGCAA+TGG - Chr1:93645984-93646003 None:intergenic 40.0%
TTGTCCCTTACATAGGAAAG+TGG + Chr1:93646810-93646829 MsG0180005586.01.T01:three_prime_UTR 40.0%
! AAAGGGTTCTCTAAGGAAGA+AGG + Chr1:93645207-93645226 MsG0180005586.01.T01:CDS 40.0%
! AACGCCACTTTCCTATGTAA+GGG - Chr1:93646817-93646836 None:intergenic 40.0%
! AACTATTAGAGGCATAACCG+AGG + Chr1:93646883-93646902 MsG0180005586.01.T01:three_prime_UTR 40.0%
! ACTTGTGCATCAAGCTTGTT+TGG - Chr1:93645763-93645782 None:intergenic 40.0%
! GATTGGTCACTCGAATTAGT+TGG + Chr1:93643198-93643217 MsG0180005586.01.T01:intron 40.0%
! GATTTTCTGAACAAGTGTGG+TGG + Chr1:93642764-93642783 MsG0180005586.01.T01:CDS 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - Chr1:93644547-93644566 None:intergenic 40.0%
! TAACGCCACTTTCCTATGTA+AGG - Chr1:93646818-93646837 None:intergenic 40.0%
!! GCCTCTAATAGTTTTTGGAG+TGG - Chr1:93646876-93646895 None:intergenic 40.0%
!! TGGAGAAGTAAAGAGGCATT+TGG - Chr1:93642867-93642886 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + Chr1:93644685-93644704 MsG0180005586.01.T01:intron 40.0%
ACTGCTCCTAGATTCATCCA+TGG + Chr1:93646028-93646047 MsG0180005586.01.T01:three_prime_UTR 45.0%
AGACATTACACCTAGCACAG+AGG - Chr1:93643569-93643588 None:intergenic 45.0%
CACCTTGATTACCTTGACTC+AGG + Chr1:93645044-93645063 MsG0180005586.01.T01:CDS 45.0%
CCTACAACAGCCACCAAAAA+GGG - Chr1:93646852-93646871 None:intergenic 45.0%
CTCCTGAGTCAAGGTAATCA+AGG - Chr1:93645049-93645068 None:intergenic 45.0%
GACACTCATTTCGAAACGGA+GGG - Chr1:93644442-93644461 None:intergenic 45.0%
GGGTATGGAATCATAGATGG+TGG + Chr1:93642785-93642804 MsG0180005586.01.T01:CDS 45.0%
GGTGGGTATGGAATCATAGA+TGG + Chr1:93642782-93642801 MsG0180005586.01.T01:CDS 45.0%
TACATATCAGACGTGGGAGT+TGG + Chr1:93646559-93646578 MsG0180005586.01.T01:intron 45.0%
TCCTACAACAGCCACCAAAA+AGG - Chr1:93646853-93646872 None:intergenic 45.0%
TTCTCCACATCTTGTCCGAA+GGG + Chr1:93642880-93642899 MsG0180005586.01.T01:CDS 45.0%
! GGCATAACCGAGGCATTATA+TGG + Chr1:93646893-93646912 MsG0180005586.01.T01:three_prime_UTR 45.0%
! GGCTTATGCTGAACTTTTGG+AGG + Chr1:93645868-93645887 MsG0180005586.01.T01:intron 45.0%
!! GCTGAACTTTTGGAGGAAGA+AGG + Chr1:93645875-93645894 MsG0180005586.01.T01:three_prime_UTR 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + Chr1:93644684-93644703 MsG0180005586.01.T01:intron 45.0%
AGGCATTTGGCACTCCAAAG+TGG - Chr1:93642854-93642873 None:intergenic 50.0%
ATAGGAAAGTGGCGTTACGC+CGG + Chr1:93646821-93646840 MsG0180005586.01.T01:three_prime_UTR 50.0%
ATCATGGAGTCCTCTGTGCT+AGG + Chr1:93643556-93643575 MsG0180005586.01.T01:intron 50.0%
CGACACTCATTTCGAAACGG+AGG - Chr1:93644443-93644462 None:intergenic 50.0%
CTTCTCCACATCTTGTCCGA+AGG + Chr1:93642879-93642898 MsG0180005586.01.T01:CDS 50.0%
GAAATCAAGTGGGGAAGCAG+AGG + Chr1:93646781-93646800 MsG0180005586.01.T01:intron 50.0%
GCAGCTTCTGTTGGAAGCTA+TGG + Chr1:93645357-93645376 MsG0180005586.01.T01:CDS 50.0%
GGCATTTGGCACTCCAAAGT+GGG - Chr1:93642853-93642872 None:intergenic 50.0%
TAGACCTCAACGACCCACTT+TGG + Chr1:93642837-93642856 MsG0180005586.01.T01:CDS 50.0%
TGCTGACAACAACACATGGG+TGG + Chr1:93646349-93646368 MsG0180005586.01.T01:three_prime_UTR 50.0%
TGTACCCTTCGGACAAGATG+TGG - Chr1:93642887-93642906 None:intergenic 50.0%
TGTTCGCAAGTTGTCGCAGT+TGG + Chr1:93645995-93646014 MsG0180005586.01.T01:three_prime_UTR 50.0%
!! CATAGATGGTGGGTTTGCCA+CGG + Chr1:93642796-93642815 MsG0180005586.01.T01:CDS 50.0%
!! CCCTTTTTGGTGGCTGTTGT+AGG + Chr1:93646849-93646868 MsG0180005586.01.T01:three_prime_UTR 50.0%
!! CTGAACAAGTGTGGTGGGTA+TGG + Chr1:93642770-93642789 MsG0180005586.01.T01:CDS 50.0%
CACTCCAAAGTGGGTCGTTG+AGG - Chr1:93642844-93642863 None:intergenic 55.0%
GGTCGTTGAGGTCTACTCCA+TGG - Chr1:93642832-93642851 None:intergenic 55.0%
TAGGAAAGTGGCGTTACGCC+GGG + Chr1:93646822-93646841 MsG0180005586.01.T01:three_prime_UTR 55.0%
AAGCGTAGAGCTTGCACGCG+AGG + Chr1:93645248-93645267 MsG0180005586.01.T01:CDS 60.0%
AGGAAAGTGGCGTTACGCCG+GGG + Chr1:93646823-93646842 MsG0180005586.01.T01:three_prime_UTR 60.0%
GCCACCAAAAAGGGAAGCCC+CGG - Chr1:93646843-93646862 None:intergenic 60.0%
TCCATGGCGTTCAAGCTCCG+TGG - Chr1:93642816-93642835 None:intergenic 60.0%
! TACGCCGGGGCTTCCCTTTT+TGG + Chr1:93646836-93646855 MsG0180005586.01.T01:three_prime_UTR 60.0%
GCCACGGAGCTTGAACGCCA+TGG + Chr1:93642812-93642831 MsG0180005586.01.T01:CDS 65.0%
! GCCGGGGCTTCCCTTTTTGG+TGG + Chr1:93646839-93646858 MsG0180005586.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 93642727 93647189 93642727 ID=MsG0180005586.01;Name=MsG0180005586.01
Chr1 mRNA 93642727 93647189 93642727 ID=MsG0180005586.01.T01;Parent=MsG0180005586.01;Name=MsG0180005586.01.T01;_AED=0.48;_eAED=0.48;_QI=28|1|1|1|1|0.83|6|1051|144
Chr1 exon 93642727 93642901 93642727 ID=MsG0180005586.01.T01:exon:10038;Parent=MsG0180005586.01.T01
Chr1 exon 93645041 93645097 93645041 ID=MsG0180005586.01.T01:exon:10039;Parent=MsG0180005586.01.T01
Chr1 exon 93645183 93645780 93645183 ID=MsG0180005586.01.T01:exon:10040;Parent=MsG0180005586.01.T01
Chr1 exon 93645869 93646072 93645869 ID=MsG0180005586.01.T01:exon:10041;Parent=MsG0180005586.01.T01
Chr1 exon 93646299 93646370 93646299 ID=MsG0180005586.01.T01:exon:10042;Parent=MsG0180005586.01.T01
Chr1 exon 93646782 93647189 93646782 ID=MsG0180005586.01.T01:exon:10043;Parent=MsG0180005586.01.T01
Chr1 five_prime_UTR 93642727 93642754 93642727 ID=MsG0180005586.01.T01:five_prime_utr;Parent=MsG0180005586.01.T01
Chr1 CDS 93642755 93642901 93642755 ID=MsG0180005586.01.T01:cds;Parent=MsG0180005586.01.T01
Chr1 CDS 93645041 93645097 93645041 ID=MsG0180005586.01.T01:cds;Parent=MsG0180005586.01.T01
Chr1 CDS 93645183 93645413 93645183 ID=MsG0180005586.01.T01:cds;Parent=MsG0180005586.01.T01
Chr1 three_prime_UTR 93645414 93645780 93645414 ID=MsG0180005586.01.T01:three_prime_utr;Parent=MsG0180005586.01.T01
Chr1 three_prime_UTR 93645869 93646072 93645869 ID=MsG0180005586.01.T01:three_prime_utr;Parent=MsG0180005586.01.T01
Chr1 three_prime_UTR 93646299 93646370 93646299 ID=MsG0180005586.01.T01:three_prime_utr;Parent=MsG0180005586.01.T01
Chr1 three_prime_UTR 93646782 93647189 93646782 ID=MsG0180005586.01.T01:three_prime_utr;Parent=MsG0180005586.01.T01
Gene Sequence

>MsG0180005586.01.T01

ATGATGAAAGATTTTCTGAACAAGTGTGGTGGGTATGGAATCATAGATGGTGGGTTTGCCACGGAGCTTGAACGCCATGGAGTAGACCTCAACGACCCACTTTGGAGTGCCAAATGCCTCTTTACTTCTCCACATCTTGTCCGAAGGGTTCACCTTGATTACCTTGACTCAGGAGCTAACATAATATTAACATCATCTTATCAGGCCACAATTCAAGGTTTTGAAGCTAAAGGGTTCTCTAAGGAAGAAGGTCAAGCTCTGCTTCGAAGAAGCGTAGAGCTTGCACGCGAGGCACGTGATATATATTATGATAGGTGCACTAAGGATTCATTTGACTTCATCAGAGATGAAAGATATAGAAGCCGTCCCATTTTAATTGCAGCTTCTGTTGGAAGCTATGGTGCCTATTTAGCTGATGGCTCTGAATATACGTGA
Protein sequence

>MsG0180005586.01.T01

MMKDFLNKCGGYGIIDGGFATELERHGVDLNDPLWSAKCLFTSPHLVRRVHLDYLDSGANIILTSSYQATIQGFEAKGFSKEEGQALLRRSVELAREARDIYYDRCTKDSFDFIRDERYRSRPILIAASVGSYGAYLADGSEYT*