AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005857.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005857.01.T01 MTR_1g062090 86.298 416 21 3 1 415 1 381 0.0 696
MsG0180005857.01.T01 MTR_4g060790 53.771 411 155 8 1 399 1 388 3.33e-137 402
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005857.01.T01 AT5G60370 44.670 394 174 8 7 398 41 392 5.29e-97 299
MsG0180005857.01.T01 AT5G60370 45.098 306 124 8 7 310 41 304 5.26e-64 211

Find 111 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGATTTGGCCTTTGTTTA+TGG 0.074187 1:+96920954 MsG0180005857.01.T01:CDS
TATGATCTCAACTGGAATTT+TGG 0.122009 1:-96920559 None:intergenic
GCAGCATCTTACACATATTT+TGG 0.169674 1:-96921502 None:intergenic
GTTCAACCACATGGAAAATT+TGG 0.173658 1:+96922193 MsG0180005857.01.T01:CDS
AGTCAACCACATGGAAAATT+TGG 0.226318 1:+96922137 MsG0180005857.01.T01:intron
CCAAATCCGGTTTGGCTTCA+TGG 0.229050 1:-96920941 None:intergenic
GCAAAATCAAAGCTTATTAT+AGG 0.231984 1:-96921161 None:intergenic
TTAGGAATTGTCTTGAGTTT+TGG 0.264173 1:+96921630 MsG0180005857.01.T01:CDS
CGAACAGCGTGTGTTGATTC+TGG 0.273513 1:+96921440 MsG0180005857.01.T01:CDS
CAGGGATACACCCCAGTTAC+TGG 0.308088 1:-96922260 None:intergenic
ACACGTTACTGCACCGGTTT+AGG 0.316965 1:+96920826 MsG0180005857.01.T01:CDS
CCATGAAGCCAAACCGGATT+TGG 0.319682 1:+96920941 MsG0180005857.01.T01:CDS
CGACTCCAAGAGGCTGATAT+TGG 0.326348 1:-96920675 None:intergenic
CTTGTCACACCACTCTGTTT+TGG 0.327688 1:-96920880 None:intergenic
GGTAATTGTAGCCCAGTAAC+TGG 0.327876 1:+96922248 MsG0180005857.01.T01:CDS
AAATTTGGCTTGGACTATAC+TGG 0.330793 1:+96922152 MsG0180005857.01.T01:CDS
AAATTTGGCTTGGACTATAC+TGG 0.330793 1:+96922208 MsG0180005857.01.T01:CDS
ATCTTCCTCATCAACATAAC+TGG 0.331924 1:-96921669 None:intergenic
GGTGGATAAAGAATGAAATT+AGG 0.335421 1:+96921612 MsG0180005857.01.T01:CDS
TCAGCTTGAACAAGAGGTTC+TGG 0.351425 1:+96921028 MsG0180005857.01.T01:CDS
GCCTCTTGGAGTCGCAAAAT+CGG 0.385198 1:+96920684 MsG0180005857.01.T01:CDS
AAACTTGTTAATTTCATCAA+TGG 0.388045 1:+96921098 MsG0180005857.01.T01:CDS
GAAGTCATCTGAAGACTATA+TGG 0.392038 1:+96921070 MsG0180005857.01.T01:CDS
AGCAGGAGTGACAGGAGAAC+GGG 0.398242 1:-96920628 None:intergenic
CTGGACATAGAGAGGGGTAT+TGG 0.428129 1:+96922171 MsG0180005857.01.T01:CDS
CTGGACATAGAGAGGGGTAT+TGG 0.428129 1:+96922227 MsG0180005857.01.T01:CDS
CAGTAACGTGTTATCTGCTC+TGG 0.432179 1:-96920814 None:intergenic
AACAAAGGCCAAATCCGGTT+TGG 0.439873 1:-96920949 None:intergenic
ACAGCAGCAGCAGGAGTGAC+AGG 0.439951 1:-96920636 None:intergenic
TCATCACTTATGATCTCAAC+TGG 0.447136 1:-96920567 None:intergenic
GACTTCACATTCACTTCCAC+TGG 0.448189 1:-96921053 None:intergenic
TGGGGAAAATTGATGAAATT+CGG 0.453588 1:+96921201 MsG0180005857.01.T01:CDS
CACCAATGTAAGCAGGGCAC+TGG 0.454243 1:-96921724 None:intergenic
AGCCCCTCTTGCTCCTAAAC+CGG 0.455720 1:-96920839 None:intergenic
ACCACATGGAAAATTTGGCT+TGG 0.460676 1:+96922198 MsG0180005857.01.T01:CDS
CTGCCAATGTCTTCTATGTC+GGG 0.463405 1:-96920765 None:intergenic
AATGTCTTCTATGTCGGGTT+CGG 0.464287 1:-96920760 None:intergenic
AACCTTCTCTTGGCTCGCAC+TGG 0.468505 1:-96920726 None:intergenic
TAATGTGTTACAAGTATTTG+TGG 0.476002 1:+96921330 MsG0180005857.01.T01:CDS
ATTATCTCTATTTGTGTCTG+TGG 0.485266 1:-96921858 None:intergenic
GAAGAACCATGAAGCCAAAC+CGG 0.486945 1:+96920935 MsG0180005857.01.T01:CDS
CCATAAACAAAGGCCAAATC+CGG 0.487026 1:-96920954 None:intergenic
ACCACATGGAAAATTTGGCT+TGG 0.490104 1:+96922142 MsG0180005857.01.T01:CDS
TTGGCCTTTGTTTATGGAGG+AGG 0.497379 1:+96920960 MsG0180005857.01.T01:CDS
AGAGTGGTGTGACAAGCAAA+TGG 0.498102 1:+96920887 MsG0180005857.01.T01:CDS
AGCATTGACACTTGCTGATG+TGG 0.498297 1:+96921469 MsG0180005857.01.T01:CDS
GATATTGGTGAATGTATAGC+AGG 0.501808 1:-96920660 None:intergenic
CACAAGGTATTTGGATGGTG+GGG 0.503716 1:+96921183 MsG0180005857.01.T01:CDS
GCACAAGGTATTTGGATGGT+GGG 0.504127 1:+96921182 MsG0180005857.01.T01:CDS
TCAGAATCACCAATGTAAGC+AGG 0.505369 1:-96921731 None:intergenic
TCCCAGTGCCCTGCTTACAT+TGG 0.505487 1:+96921722 MsG0180005857.01.T01:CDS
ATTCACCAATATCAGCCTCT+TGG 0.505967 1:+96920670 MsG0180005857.01.T01:CDS
TGAGTCATCATACTCTGAAA+GGG 0.510429 1:-96921765 None:intergenic
CACTAGCTTATCATTAGCAC+GGG 0.510557 1:-96921525 None:intergenic
ACTGCCAATGTCTTCTATGT+CGG 0.516993 1:-96920766 None:intergenic
TTGAGTCATCATACTCTGAA+AGG 0.523686 1:-96921766 None:intergenic
AGAAGCTAAAGCAGCATCAA+TGG 0.525186 1:-96920601 None:intergenic
TATTCAAACAATCTTTCAGA+AGG 0.525726 1:-96921377 None:intergenic
AATGTGTTACAAGTATTTGT+GGG 0.525943 1:+96921331 MsG0180005857.01.T01:CDS
TTGTTTATGGAGGAGGAAGT+TGG 0.529333 1:+96920967 MsG0180005857.01.T01:CDS
GGATCAGCTACCAGAATGGC+AGG 0.530509 1:+96920528 None:intergenic
GTAATTGTAGCCCAGTAACT+GGG 0.531789 1:+96922249 MsG0180005857.01.T01:CDS
GTCTTAGTTTCAACTAGCAT+CGG 0.533684 1:-96921251 None:intergenic
CAGCAGGAGTGACAGGAGAA+CGG 0.534522 1:-96920629 None:intergenic
AAGAGTAAAGCGATGAAAGC+TGG 0.536293 1:+96920990 MsG0180005857.01.T01:CDS
ACACGAGAATAACCTTCTCT+TGG 0.536798 1:-96920736 None:intergenic
GTGAATCAACTGTTGTTTGA+AGG 0.539135 1:+96921122 MsG0180005857.01.T01:CDS
TGCACAAGGTATTTGGATGG+TGG 0.540504 1:+96921181 MsG0180005857.01.T01:CDS
AGAAGCCAGTTATGTTGATG+AGG 0.540672 1:+96921664 MsG0180005857.01.T01:CDS
CAGATAACACGTTACTGCAC+CGG 0.541368 1:+96920820 MsG0180005857.01.T01:CDS
ATAGCAGGAACAGCAGCAGC+AGG 0.544511 1:-96920645 None:intergenic
TGATGAAATTCGGATGCCTA+AGG 0.544579 1:+96921211 MsG0180005857.01.T01:CDS
TCAGAGGGATCACTCACTGT+TGG 0.546666 1:+96921562 MsG0180005857.01.T01:CDS
GACTATACTGGACATAGAGA+GGG 0.551387 1:+96922164 MsG0180005857.01.T01:CDS
GACTATACTGGACATAGAGA+GGG 0.551387 1:+96922220 MsG0180005857.01.T01:CDS
CGATGAAAGCTGGTATAGAT+CGG 0.566862 1:+96921000 MsG0180005857.01.T01:CDS
ACCAATGTAAGCAGGGCACT+GGG 0.567241 1:-96921723 None:intergenic
TTCCAGTGCGAGCCAAGAGA+AGG 0.567406 1:+96920724 MsG0180005857.01.T01:CDS
ATTCTGCAGGAACTGTATCT+CGG 0.580937 1:-96921280 None:intergenic
GGGGTATTGGTTCAACCACA+TGG 0.584857 1:+96922184 MsG0180005857.01.T01:CDS
GAGGAAGATCAATGGAAATG+TGG 0.585803 1:+96921683 MsG0180005857.01.T01:CDS
AGGGATACACCCCAGTTACT+GGG 0.586516 1:-96922259 None:intergenic
ATTCAAACAATCTTTCAGAA+GGG 0.587293 1:-96921376 None:intergenic
ATTGACACTTGCTGATGTGG+TGG 0.594742 1:+96921472 MsG0180005857.01.T01:CDS
ATGTTGATGAGGAAGATCAA+TGG 0.596284 1:+96921675 MsG0180005857.01.T01:CDS
ACACTAGCTTATCATTAGCA+CGG 0.607468 1:-96921526 None:intergenic
CACCGGTTTAGGAGCAAGAG+GGG 0.610660 1:+96920837 MsG0180005857.01.T01:CDS
CAGAATCACCAATGTAAGCA+GGG 0.612970 1:-96921730 None:intergenic
GATTTGGCCTTTGTTTATGG+AGG 0.613744 1:+96920957 MsG0180005857.01.T01:CDS
TGCACCGGTTTAGGAGCAAG+AGG 0.615572 1:+96920835 MsG0180005857.01.T01:CDS
GATCATAAGTGATGATGAGA+TGG 0.617193 1:+96920575 MsG0180005857.01.T01:CDS
TTCTTACACAGTCAACCACA+TGG 0.617698 1:+96922128 MsG0180005857.01.T01:intron
GGACTATACTGGACATAGAG+AGG 0.618217 1:+96922163 MsG0180005857.01.T01:CDS
GGACTATACTGGACATAGAG+AGG 0.618217 1:+96922219 MsG0180005857.01.T01:CDS
TGGAATGGTTAGGATGTCAA+TGG 0.621029 1:+96921831 MsG0180005857.01.T01:intron
GTTGAGATACGAATCTCAGA+GGG 0.624591 1:+96921547 MsG0180005857.01.T01:CDS
TCGCACTGGAACTGACTGAA+AGG 0.627807 1:-96920712 None:intergenic
GCACCGGTTTAGGAGCAAGA+GGG 0.640558 1:+96920836 MsG0180005857.01.T01:CDS
ACTTCCTCCTCCATAAACAA+AGG 0.641083 1:-96920964 None:intergenic
ACAAGAGGTTCTGGAACCAG+TGG 0.648454 1:+96921037 MsG0180005857.01.T01:CDS
AATTTGTGTATGATGGTAGG+TGG 0.653126 1:+96921594 MsG0180005857.01.T01:CDS
TCTTTACATAAATTACGTCG+AGG 0.667263 1:-96921413 None:intergenic
GAACCCGACATAGAAGACAT+TGG 0.671635 1:+96920762 MsG0180005857.01.T01:CDS
TGTTGAGATACGAATCTCAG+AGG 0.675787 1:+96921546 MsG0180005857.01.T01:CDS
TAATTGTAGCCCAGTAACTG+GGG 0.698920 1:+96922250 MsG0180005857.01.T01:CDS
AACATCAATACATTACCACT+AGG 0.706106 1:-96922385 None:intergenic
ACTATACTGGACATAGAGAG+GGG 0.715849 1:+96922165 MsG0180005857.01.T01:CDS
ACTATACTGGACATAGAGAG+GGG 0.715849 1:+96922221 MsG0180005857.01.T01:CDS
ATACTCATTTGCTATCCGCG+CGG 0.741077 1:+96921879 MsG0180005857.01.T01:CDS
CCGATATCACCAAAACAGAG+TGG 0.741120 1:+96920871 MsG0180005857.01.T01:CDS
ACTAGCTTATCATTAGCACG+GGG 0.797096 1:-96921524 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTTTAAATTGTTAGA+AGG - Chr1:96922109-96922128 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTTGTC+AGG + Chr1:96922359-96922378 MsG0180005857.01.T01:intron 10.0%
!! TACTGAATTATGAATAATAC+TGG - Chr1:96922070-96922089 None:intergenic 20.0%
!! TGTTCATAATGAAAAATACA+GGG - Chr1:96922281-96922300 None:intergenic 20.0%
!! TTGTTCATAATGAAAAATAC+AGG - Chr1:96922282-96922301 None:intergenic 20.0%
!!! AAACTTGTTAATTTCATCAA+TGG + Chr1:96921098-96921117 MsG0180005857.01.T01:CDS 20.0%
!!! ATTAAACTTCAATTTTCGTA+AGG - Chr1:96922330-96922349 None:intergenic 20.0%
!!! TTAAACTTCAATTTTCGTAA+GGG - Chr1:96922329-96922348 None:intergenic 20.0%
! AAAAATTTGTGTATGATGGT+AGG + Chr1:96921591-96921610 MsG0180005857.01.T01:CDS 25.0%
! AAGGGAAAAGAAAATATAGT+GGG - Chr1:96922311-96922330 None:intergenic 25.0%
! AATGTGTTACAAGTATTTGT+GGG + Chr1:96921331-96921350 MsG0180005857.01.T01:CDS 25.0%
! ATTCAAACAATCTTTCAGAA+GGG - Chr1:96921379-96921398 None:intergenic 25.0%
! GAAGAAAAATTTGTGTATGA+TGG + Chr1:96921587-96921606 MsG0180005857.01.T01:CDS 25.0%
! GCAAAATCAAAGCTTATTAT+AGG - Chr1:96921164-96921183 None:intergenic 25.0%
! TAAGGGAAAAGAAAATATAG+TGG - Chr1:96922312-96922331 None:intergenic 25.0%
! TAATGTGTTACAAGTATTTG+TGG + Chr1:96921330-96921349 MsG0180005857.01.T01:CDS 25.0%
! TATTCAAACAATCTTTCAGA+AGG - Chr1:96921380-96921399 None:intergenic 25.0%
!!! TTATGATCTTTTTTTGCCTT+AGG - Chr1:96921230-96921249 None:intergenic 25.0%
AGGGAAAAGAAAATATAGTG+GGG - Chr1:96922310-96922329 None:intergenic 30.0%
ATTATCTCTATTTGTGTCTG+TGG - Chr1:96921861-96921880 None:intergenic 30.0%
CAGAATCACAAAAAAGAAAC+GGG + Chr1:96921297-96921316 MsG0180005857.01.T01:CDS 30.0%
GCAGAATCACAAAAAAGAAA+CGG + Chr1:96921296-96921315 MsG0180005857.01.T01:CDS 30.0%
GGTGGATAAAGAATGAAATT+AGG + Chr1:96921612-96921631 MsG0180005857.01.T01:CDS 30.0%
GTGTTCAATAAGTTCTGTTA+TGG + Chr1:96921801-96921820 MsG0180005857.01.T01:intron 30.0%
TCTTTACATAAATTACGTCG+AGG - Chr1:96921416-96921435 None:intergenic 30.0%
TGGGGAAAATTGATGAAATT+CGG + Chr1:96921201-96921220 MsG0180005857.01.T01:CDS 30.0%
! TACTTTCTAGCGTAATCTTA+TGG - Chr1:96922022-96922041 None:intergenic 30.0%
! TATGATCTCAACTGGAATTT+TGG - Chr1:96920562-96920581 None:intergenic 30.0%
! TTAGGAATTGTCTTGAGTTT+TGG + Chr1:96921630-96921649 MsG0180005857.01.T01:CDS 30.0%
!! ATAAGCTTTGATTTTGCACA+AGG + Chr1:96921167-96921186 MsG0180005857.01.T01:CDS 30.0%
!! TTGATTTTGCACAAGGTATT+TGG + Chr1:96921174-96921193 MsG0180005857.01.T01:CDS 30.0%
!!! TTTCGCTCTTTTTTGAAGAA+TGG + Chr1:96920913-96920932 MsG0180005857.01.T01:CDS 30.0%
!!! TTTCTTTTTTGTGATTCTGC+AGG - Chr1:96921296-96921315 None:intergenic 30.0%
AATCACAAAAAAGAAACGGG+AGG + Chr1:96921300-96921319 MsG0180005857.01.T01:CDS 35.0%
AATTTGTGTATGATGGTAGG+TGG + Chr1:96921594-96921613 MsG0180005857.01.T01:CDS 35.0%
AGTCAACCACATGGAAAATT+TGG + Chr1:96922137-96922156 MsG0180005857.01.T01:intron 35.0%
ATCTTCCTCATCAACATAAC+TGG - Chr1:96921672-96921691 None:intergenic 35.0%
GAAGTCATCTGAAGACTATA+TGG + Chr1:96921070-96921089 MsG0180005857.01.T01:CDS 35.0%
GATATTGGTGAATGTATAGC+AGG - Chr1:96920663-96920682 None:intergenic 35.0%
GTCTTAGTTTCAACTAGCAT+CGG - Chr1:96921254-96921273 None:intergenic 35.0%
GTTCAACCACATGGAAAATT+TGG + Chr1:96922193-96922212 MsG0180005857.01.T01:CDS 35.0%
TCATCACTTATGATCTCAAC+TGG - Chr1:96920570-96920589 None:intergenic 35.0%
TTGAGTCATCATACTCTGAA+AGG - Chr1:96921769-96921788 None:intergenic 35.0%
! ACACTAGCTTATCATTAGCA+CGG - Chr1:96921529-96921548 None:intergenic 35.0%
! ATGTTGATGAGGAAGATCAA+TGG + Chr1:96921675-96921694 MsG0180005857.01.T01:CDS 35.0%
! GATCATAAGTGATGATGAGA+TGG + Chr1:96920575-96920594 MsG0180005857.01.T01:CDS 35.0%
! GCAGCATCTTACACATATTT+TGG - Chr1:96921505-96921524 None:intergenic 35.0%
! GTGAATCAACTGTTGTTTGA+AGG + Chr1:96921122-96921141 MsG0180005857.01.T01:CDS 35.0%
! TGAGTCATCATACTCTGAAA+GGG - Chr1:96921768-96921787 None:intergenic 35.0%
!! AAATTTGGCTTGGACTATAC+TGG + Chr1:96922152-96922171 MsG0180005857.01.T01:CDS 35.0%
!! TTTTGCACAAGGTATTTGGA+TGG + Chr1:96921178-96921197 MsG0180005857.01.T01:CDS 35.0%
!!! AATTGTCTTGAGTTTTGGCT+TGG + Chr1:96921635-96921654 MsG0180005857.01.T01:CDS 35.0%
AAGAGTAAAGCGATGAAAGC+TGG + Chr1:96920990-96921009 MsG0180005857.01.T01:CDS 40.0%
AATGTCTTCTATGTCGGGTT+CGG - Chr1:96920763-96920782 None:intergenic 40.0%
ACACGAGAATAACCTTCTCT+TGG - Chr1:96920739-96920758 None:intergenic 40.0%
ACCACATGGAAAATTTGGCT+TGG + Chr1:96922142-96922161 MsG0180005857.01.T01:CDS 40.0%
ACTATACTGGACATAGAGAG+GGG + Chr1:96922165-96922184 MsG0180005857.01.T01:CDS 40.0%
ACTGCCAATGTCTTCTATGT+CGG - Chr1:96920769-96920788 None:intergenic 40.0%
ACTTCCTCCTCCATAAACAA+AGG - Chr1:96920967-96920986 None:intergenic 40.0%
AGAAGCCAGTTATGTTGATG+AGG + Chr1:96921664-96921683 MsG0180005857.01.T01:CDS 40.0%
AGAAGCTAAAGCAGCATCAA+TGG - Chr1:96920604-96920623 None:intergenic 40.0%
AGTTCTGTTATGGTTTCTGC+TGG + Chr1:96921811-96921830 MsG0180005857.01.T01:intron 40.0%
ATTCACCAATATCAGCCTCT+TGG + Chr1:96920670-96920689 MsG0180005857.01.T01:CDS 40.0%
ATTCTGCAGGAACTGTATCT+CGG - Chr1:96921283-96921302 None:intergenic 40.0%
CAGAATCACCAATGTAAGCA+GGG - Chr1:96921733-96921752 None:intergenic 40.0%
CCATAAACAAAGGCCAAATC+CGG - Chr1:96920957-96920976 None:intergenic 40.0%
CGATGAAAGCTGGTATAGAT+CGG + Chr1:96921000-96921019 MsG0180005857.01.T01:CDS 40.0%
GACTATACTGGACATAGAGA+GGG + Chr1:96922164-96922183 MsG0180005857.01.T01:CDS 40.0%
GAGGAAGATCAATGGAAATG+TGG + Chr1:96921683-96921702 MsG0180005857.01.T01:CDS 40.0%
GTAATTGTAGCCCAGTAACT+GGG + Chr1:96922249-96922268 MsG0180005857.01.T01:CDS 40.0%
GTTGAGATACGAATCTCAGA+GGG + Chr1:96921547-96921566 MsG0180005857.01.T01:CDS 40.0%
TAATTGTAGCCCAGTAACTG+GGG + Chr1:96922250-96922269 MsG0180005857.01.T01:CDS 40.0%
TCAGAATCACCAATGTAAGC+AGG - Chr1:96921734-96921753 None:intergenic 40.0%
TGATGAAATTCGGATGCCTA+AGG + Chr1:96921211-96921230 MsG0180005857.01.T01:CDS 40.0%
TGGAATGGTTAGGATGTCAA+TGG + Chr1:96921831-96921850 MsG0180005857.01.T01:intron 40.0%
TGTTATGGTTTCTGCTGGAA+TGG + Chr1:96921816-96921835 MsG0180005857.01.T01:intron 40.0%
TGTTGAGATACGAATCTCAG+AGG + Chr1:96921546-96921565 MsG0180005857.01.T01:CDS 40.0%
TTCTTACACAGTCAACCACA+TGG + Chr1:96922128-96922147 MsG0180005857.01.T01:intron 40.0%
TTGTTTATGGAGGAGGAAGT+TGG + Chr1:96920967-96920986 MsG0180005857.01.T01:CDS 40.0%
! ACTAGCTTATCATTAGCACG+GGG - Chr1:96921527-96921546 None:intergenic 40.0%
! CACTAGCTTATCATTAGCAC+GGG - Chr1:96921528-96921547 None:intergenic 40.0%
! GATCTCAACTGGAATTTTGG+AGG - Chr1:96920559-96920578 None:intergenic 40.0%
! GATTTGGCCTTTGTTTATGG+AGG + Chr1:96920957-96920976 MsG0180005857.01.T01:CDS 40.0%
! GCATTTTCAGCTTGAACAAG+AGG + Chr1:96921022-96921041 MsG0180005857.01.T01:CDS 40.0%
! TCCAAGCCAAATTTTCCATG+TGG - Chr1:96922146-96922165 None:intergenic 40.0%
!!! CCACTCTGTTTTGGTGATAT+CGG - Chr1:96920874-96920893 None:intergenic 40.0%
!!! TTTTTTGTCAGGTAGCCTAG+TGG + Chr1:96922370-96922389 MsG0180005857.01.T01:intron 40.0%
AACAAAGGCCAAATCCGGTT+TGG - Chr1:96920952-96920971 None:intergenic 45.0%
AGAGTGGTGTGACAAGCAAA+TGG + Chr1:96920887-96920906 MsG0180005857.01.T01:CDS 45.0%
CAGATAACACGTTACTGCAC+CGG + Chr1:96920820-96920839 MsG0180005857.01.T01:CDS 45.0%
CAGTAACGTGTTATCTGCTC+TGG - Chr1:96920817-96920836 None:intergenic 45.0%
CCGATATCACCAAAACAGAG+TGG + Chr1:96920871-96920890 MsG0180005857.01.T01:CDS 45.0%
CTGCCAATGTCTTCTATGTC+GGG - Chr1:96920768-96920787 None:intergenic 45.0%
GAACCCGACATAGAAGACAT+TGG + Chr1:96920762-96920781 MsG0180005857.01.T01:CDS 45.0%
GAAGAACCATGAAGCCAAAC+CGG + Chr1:96920935-96920954 MsG0180005857.01.T01:CDS 45.0%
GACTTCACATTCACTTCCAC+TGG - Chr1:96921056-96921075 None:intergenic 45.0%
GGACTATACTGGACATAGAG+AGG + Chr1:96922163-96922182 MsG0180005857.01.T01:CDS 45.0%
GGTAATTGTAGCCCAGTAAC+TGG + Chr1:96922248-96922267 MsG0180005857.01.T01:CDS 45.0%
TCAGCTTGAACAAGAGGTTC+TGG + Chr1:96921028-96921047 MsG0180005857.01.T01:CDS 45.0%
TGGTTTCTGCTGGAATGGTT+AGG + Chr1:96921821-96921840 MsG0180005857.01.T01:intron 45.0%
! ATTGACACTTGCTGATGTGG+TGG + Chr1:96921472-96921491 MsG0180005857.01.T01:CDS 45.0%
! CACAAGGTATTTGGATGGTG+GGG + Chr1:96921183-96921202 MsG0180005857.01.T01:CDS 45.0%
! CCGGATTTGGCCTTTGTTTA+TGG + Chr1:96920954-96920973 MsG0180005857.01.T01:CDS 45.0%
! CTTGTCACACCACTCTGTTT+TGG - Chr1:96920883-96920902 None:intergenic 45.0%
! GCACAAGGTATTTGGATGGT+GGG + Chr1:96921182-96921201 MsG0180005857.01.T01:CDS 45.0%
! TGCACAAGGTATTTGGATGG+TGG + Chr1:96921181-96921200 MsG0180005857.01.T01:CDS 45.0%
! TTGGCCTTTGTTTATGGAGG+AGG + Chr1:96920960-96920979 MsG0180005857.01.T01:CDS 45.0%
!! AGCATTGACACTTGCTGATG+TGG + Chr1:96921469-96921488 MsG0180005857.01.T01:CDS 45.0%
!! ATACTCATTTGCTATCCGCG+CGG + Chr1:96921879-96921898 MsG0180005857.01.T01:CDS 45.0%
AGGGATACACCCCAGTTACT+GGG - Chr1:96922262-96922281 None:intergenic 50.0%
CCATGAAGCCAAACCGGATT+TGG + Chr1:96920941-96920960 MsG0180005857.01.T01:CDS 50.0%
CGACTCCAAGAGGCTGATAT+TGG - Chr1:96920678-96920697 None:intergenic 50.0%
GCCTCTTGGAGTCGCAAAAT+CGG + Chr1:96920684-96920703 MsG0180005857.01.T01:CDS 50.0%
TCAGAGGGATCACTCACTGT+TGG + Chr1:96921562-96921581 MsG0180005857.01.T01:CDS 50.0%
TCGCACTGGAACTGACTGAA+AGG - Chr1:96920715-96920734 None:intergenic 50.0%
! ACCAATGTAAGCAGGGCACT+GGG - Chr1:96921726-96921745 None:intergenic 50.0%
! CTGGACATAGAGAGGGGTAT+TGG + Chr1:96922171-96922190 MsG0180005857.01.T01:CDS 50.0%
!! ACAAGAGGTTCTGGAACCAG+TGG + Chr1:96921037-96921056 MsG0180005857.01.T01:CDS 50.0%
!! ACACGTTACTGCACCGGTTT+AGG + Chr1:96920826-96920845 MsG0180005857.01.T01:CDS 50.0%
!! CCAAATCCGGTTTGGCTTCA+TGG - Chr1:96920944-96920963 None:intergenic 50.0%
!! CGAACAGCGTGTGTTGATTC+TGG + Chr1:96921440-96921459 MsG0180005857.01.T01:CDS 50.0%
!! GGGGTATTGGTTCAACCACA+TGG + Chr1:96922184-96922203 MsG0180005857.01.T01:CDS 50.0%
AGCAGGAGTGACAGGAGAAC+GGG - Chr1:96920631-96920650 None:intergenic 55.0%
AGCCCCTCTTGCTCCTAAAC+CGG - Chr1:96920842-96920861 None:intergenic 55.0%
ATAGCAGGAACAGCAGCAGC+AGG - Chr1:96920648-96920667 None:intergenic 55.0%
CACCAATGTAAGCAGGGCAC+TGG - Chr1:96921727-96921746 None:intergenic 55.0%
CAGCAGGAGTGACAGGAGAA+CGG - Chr1:96920632-96920651 None:intergenic 55.0%
CAGGGATACACCCCAGTTAC+TGG - Chr1:96922263-96922282 None:intergenic 55.0%
TCCCAGTGCCCTGCTTACAT+TGG + Chr1:96921722-96921741 MsG0180005857.01.T01:CDS 55.0%
TTCCAGTGCGAGCCAAGAGA+AGG + Chr1:96920724-96920743 MsG0180005857.01.T01:CDS 55.0%
! AACCTTCTCTTGGCTCGCAC+TGG - Chr1:96920729-96920748 None:intergenic 55.0%
!! CACCGGTTTAGGAGCAAGAG+GGG + Chr1:96920837-96920856 MsG0180005857.01.T01:CDS 55.0%
!! GCACCGGTTTAGGAGCAAGA+GGG + Chr1:96920836-96920855 MsG0180005857.01.T01:CDS 55.0%
!! GCCGATTTTGCGACTCCAAG+AGG - Chr1:96920688-96920707 None:intergenic 55.0%
!! TCATTTGCTATCCGCGCGGT+TGG + Chr1:96921883-96921902 MsG0180005857.01.T01:intron 55.0%
!! TGCACCGGTTTAGGAGCAAG+AGG + Chr1:96920835-96920854 MsG0180005857.01.T01:CDS 55.0%
ACAGCAGCAGCAGGAGTGAC+AGG - Chr1:96920639-96920658 None:intergenic 60.0%
TTGCTATCCGCGCGGTTGGA+GGG + Chr1:96921887-96921906 MsG0180005857.01.T01:intron 60.0%
! TTTGCTATCCGCGCGGTTGG+AGG + Chr1:96921886-96921905 MsG0180005857.01.T01:intron 60.0%
AAAGAGCCCCTCCAACCGCG+CGG - Chr1:96921897-96921916 None:intergenic 65.0%
TGCTATCCGCGCGGTTGGAG+GGG + Chr1:96921888-96921907 MsG0180005857.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 96920543 96922403 96920543 ID=MsG0180005857.01;Name=MsG0180005857.01
Chr1 mRNA 96920543 96922403 96920543 ID=MsG0180005857.01.T01;Parent=MsG0180005857.01;Name=MsG0180005857.01.T01;_AED=0.41;_eAED=0.50;_QI=0|0|0|1|1|1|4|0|489
Chr1 exon 96920543 96921788 96920543 ID=MsG0180005857.01.T01:exon:16246;Parent=MsG0180005857.01.T01
Chr1 exon 96921843 96921900 96921843 ID=MsG0180005857.01.T01:exon:16247;Parent=MsG0180005857.01.T01
Chr1 exon 96922139 96922281 96922139 ID=MsG0180005857.01.T01:exon:16248;Parent=MsG0180005857.01.T01
Chr1 exon 96922381 96922403 96922381 ID=MsG0180005857.01.T01:exon:16249;Parent=MsG0180005857.01.T01
Chr1 CDS 96920543 96921788 96920543 ID=MsG0180005857.01.T01:cds;Parent=MsG0180005857.01.T01
Chr1 CDS 96921843 96921900 96921843 ID=MsG0180005857.01.T01:cds;Parent=MsG0180005857.01.T01
Chr1 CDS 96922139 96922281 96922139 ID=MsG0180005857.01.T01:cds;Parent=MsG0180005857.01.T01
Chr1 CDS 96922381 96922403 96922381 ID=MsG0180005857.01.T01:cds;Parent=MsG0180005857.01.T01
Gene Sequence

>MsG0180005857.01.T01

ATGGCAGGAACATCCTCCAAAATTCCAGTTGAGATCATAAGTGATGATGAGATGGCTTCCATTGATGCTGCTTTAGCTTCTGCAGCCCGTTCTCCTGTCACTCCTGCTGCTGCTGTTCCTGCTATACATTCACCAATATCAGCCTCTTGGAGTCGCAAAATCGGCACATCCTTTCAGTCAGTTCCAGTGCGAGCCAAGAGAAGGTTATTCTCGTGTTCCGAACCCGACATAGAAGACATTGGCAGTTTTTTAAGTGCTAAGAAGAAGAAGTCCAGAGCAGATAACACGTTACTGCACCGGTTTAGGAGCAAGAGGGGCTTGTTTGTCACCGATATCACCAAAACAGAGTGGTGTGACAAGCAAATGGAATTTTCGCTCTTTTTTGAAGAATGGAAGAACCATGAAGCCAAACCGGATTTGGCCTTTGTTTATGGAGGAGGAAGTTGGAAGAGTAAAGCGATGAAAGCTGGTATAGATCGGCATTTTCAGCTTGAACAAGAGGTTCTGGAACCAGTGGAAGTGAATGTGAAGTCATCTGAAGACTATATGGCATTGAAACTTGTTAATTTCATCAATGGTGTGAATCAACTGTTGTTTGAAGGACTAACTCGCGAGCTTCCTATAATAAGCTTTGATTTTGCACAAGGTATTTGGATGGTGGGGAAAATTGATGAAATTCGGATGCCTAAGGCAAAAAAAGATCATAATCCGATGCTAGTTGAAACTAAGACTCGTTACCGAGATACAGTTCCTGCAGAATCACAAAAAAGAAACGGGAGGATTCAACTAATGTGTTACAAGTATTTGTGGGACAATTTAGTTGTTCGTGACTTCCCTTCTGAAAGATTGTTTGAATATTTCGAGTTAAATCCTCGACGTAATTTATGTAAAGATCTACGAACAGCGTGTGTTGATTCTGGAATCTCAGCATTGACACTTGCTGATGTGGTGGTTTGTTACCAAAATATGTGTAAGATGCTGCCCCGTGCTAATGATAAGCTAGTGTTGAGATACGAATCTCAGAGGGATCACTCACTGTTGGAAGAAGAAAAATTTGTGTATGATGGTAGGTGGATAAAGAATGAAATTAGGAATTGTCTTGAGTTTTGGCTTGGAAAGAGAGAAGCCAGTTATGTTGATGAGGAAGATCAATGGAAATGTGGATTTTGTGATTTTACTTCCCAGTGCCCTGCTTACATTGGTGATTCTGAGAGCACAGAAACCCTTTCAGAGTATGATGACTCAAGATGTCAATGGATATCCACAGACACAAATAGAGATAATACTCATTTGCTATCCGCGCGTCAACCACATGGAAAATTTGGCTTGGACTATACTGGACATAGAGAGGGGTATTGGTTCAACCACATGGAAAATTTGGCTTGGACTATACTGGACATAGAGAGGGGTATTGGTAATTGTAGCCCAGTAACTGGGGTGTATCCCTGTAGCCTAGTGGTAATGTATTGA
Protein sequence

>MsG0180005857.01.T01

MAGTSSKIPVEIISDDEMASIDAALASAARSPVTPAAAVPAIHSPISASWSRKIGTSFQSVPVRAKRRLFSCSEPDIEDIGSFLSAKKKKSRADNTLLHRFRSKRGLFVTDITKTEWCDKQMEFSLFFEEWKNHEAKPDLAFVYGGGSWKSKAMKAGIDRHFQLEQEVLEPVEVNVKSSEDYMALKLVNFINGVNQLLFEGLTRELPIISFDFAQGIWMVGKIDEIRMPKAKKDHNPMLVETKTRYRDTVPAESQKRNGRIQLMCYKYLWDNLVVRDFPSERLFEYFELNPRRNLCKDLRTACVDSGISALTLADVVVCYQNMCKMLPRANDKLVLRYESQRDHSLLEEEKFVYDGRWIKNEIRNCLEFWLGKREASYVDEEDQWKCGFCDFTSQCPAYIGDSESTETLSEYDDSRCQWISTDTNRDNTHLLSARQPHGKFGLDYTGHREGYWFNHMENLAWTILDIERGIGNCSPVTGVYPCSLVVMY*