AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006335.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006335.01.T01 MTR_2g006280 66.434 286 42 7 5 284 44 281 6.10e-104 319
MsG0280006335.01.T01 MTR_2g006280 93.960 149 4 1 284 427 403 551 6.63e-88 278
MsG0280006335.01.T01 MTR_5g022400 35.569 343 149 12 7 324 47 342 2.20e-43 156
MsG0280006335.01.T01 MTR_5g022400 35.569 343 149 12 7 324 47 342 1.76e-42 157
MsG0280006335.01.T01 MTR_5g022400 47.368 76 37 1 281 353 407 482 2.20e-12 69.3
MsG0280006335.01.T01 MTR_2g079500 35.714 294 117 10 5 284 44 279 1.06e-34 135
MsG0280006335.01.T01 MTR_3g115940 29.412 289 144 11 7 285 46 284 1.76e-19 90.9
MsG0280006335.01.T01 MTR_3g115940 29.412 289 144 11 7 285 46 284 2.65e-19 90.5
MsG0280006335.01.T01 MTR_3g115940 29.412 289 144 11 7 285 46 284 3.85e-19 89.0
MsG0280006335.01.T01 MTR_2g093750 26.912 353 161 14 5 311 43 344 1.18e-18 88.6
MsG0280006335.01.T01 MTR_3g118190 29.153 295 146 9 5 284 47 293 6.47e-18 86.3
MsG0280006335.01.T01 MTR_3g115940 29.286 280 138 11 16 285 4 233 6.56e-18 86.3
MsG0280006335.01.T01 MTR_6g033330 28.065 310 153 14 1 291 37 295 1.05e-17 85.9
MsG0280006335.01.T01 MTR_6g033330 26.836 354 164 16 1 310 37 339 1.53e-17 84.3
MsG0280006335.01.T01 MTR_2g028470 28.920 287 142 9 5 284 43 274 5.09e-16 80.1
MsG0280006335.01.T01 MTR_5g024700 27.393 303 138 15 7 292 65 302 1.11e-15 79.3
MsG0280006335.01.T01 MTR_8g010530 27.157 313 140 12 4 284 46 302 2.78e-15 78.2
MsG0280006335.01.T01 MTR_8g073130 32.520 123 69 2 10 126 78 192 1.63e-11 66.6
MsG0280006335.01.T01 MTR_8g073130 32.520 123 69 2 10 126 78 192 2.88e-11 65.5
MsG0280006335.01.T01 MTR_8g073130 32.520 123 69 2 10 126 78 192 3.83e-11 65.1
MsG0280006335.01.T01 MTR_8g010500 34.058 138 69 3 5 123 45 179 8.05e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006335.01.T01 AT1G11000 49.477 287 87 9 5 284 44 279 1.65e-65 216
MsG0280006335.01.T01 AT1G11000 49.477 287 87 9 5 284 44 279 1.19e-64 218
MsG0280006335.01.T01 AT1G11000 56.204 137 45 5 284 412 402 531 4.74e-36 140
MsG0280006335.01.T01 AT1G11000 49.477 287 87 9 5 284 44 279 1.19e-64 218
MsG0280006335.01.T01 AT1G11000 56.204 137 45 5 284 412 402 531 4.74e-36 140
MsG0280006335.01.T01 AT5G53760 35.932 295 121 10 5 284 51 292 3.14e-40 151
MsG0280006335.01.T01 AT5G53760 35.932 295 121 10 5 284 51 292 3.14e-40 151
MsG0280006335.01.T01 AT1G26700 34.576 295 126 9 5 284 45 287 1.63e-38 146
MsG0280006335.01.T01 AT1G26700 34.576 295 126 9 5 284 45 287 1.63e-38 146
MsG0280006335.01.T01 AT2G33670 27.719 285 148 9 4 284 49 279 2.82e-19 90.5
MsG0280006335.01.T01 AT1G61560 26.781 351 162 14 6 311 44 344 4.98e-19 89.7
MsG0280006335.01.T01 AT1G61560 26.781 351 162 14 6 311 44 344 5.16e-19 89.7
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 5.44e-19 89.0
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 5.44e-19 89.0
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 5.44e-19 89.0
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 6.70e-19 89.0
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 6.71e-19 88.6
MsG0280006335.01.T01 AT1G61560 26.286 350 165 14 6 311 44 344 6.71e-19 88.6
MsG0280006335.01.T01 AT4G24250 28.374 289 149 8 5 284 42 281 2.43e-18 87.4
MsG0280006335.01.T01 AT4G24250 28.374 289 149 8 5 284 42 281 4.88e-18 85.9
MsG0280006335.01.T01 AT5G65970 27.241 290 143 9 10 294 74 300 1.07e-17 85.9
MsG0280006335.01.T01 AT2G44110 25.263 285 159 9 6 284 42 278 3.01e-17 84.3
MsG0280006335.01.T01 AT5G65970 27.622 286 147 9 10 294 74 300 3.51e-17 84.0
MsG0280006335.01.T01 AT1G42560 27.027 296 142 12 4 283 48 285 1.02e-16 81.6
MsG0280006335.01.T01 AT2G39200 26.571 350 160 16 6 311 42 338 1.14e-16 82.8
MsG0280006335.01.T01 AT4G02600 28.571 301 145 13 4 284 42 292 2.14e-16 81.6
MsG0280006335.01.T01 AT4G02600 28.571 301 145 13 4 284 42 292 2.14e-16 81.6
MsG0280006335.01.T01 AT2G17430 25.075 335 168 14 7 318 68 342 3.23e-16 81.3
MsG0280006335.01.T01 AT1G42560 27.027 296 142 12 4 283 48 285 3.24e-16 80.9
MsG0280006335.01.T01 AT2G44110 25.263 285 158 10 6 284 42 277 7.21e-16 80.1

Find 78 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCATCGATCGTTGATTCT+AGG 0.269205 2:-603518 None:intergenic
TTCATGGCATTCTAGGAATA+AGG 0.290919 2:+602156 MsG0280006335.01.T01:CDS
ACATGAACATTGACCATCTT+AGG 0.311651 2:-600811 None:intergenic
CCTGTGAAACTATTACATTC+AGG 0.321958 2:-603218 None:intergenic
GCAGTGTTCTTGTTCAGTTC+TGG 0.326834 2:+603175 MsG0280006335.01.T01:intron
TGGCTCTTATCGATGGAAAT+TGG 0.341666 2:+601332 MsG0280006335.01.T01:CDS
GACGAATTTCATGGCATTCT+AGG 0.354070 2:+602149 MsG0280006335.01.T01:CDS
ATTCAAAGACTCTAAGTCTA+AGG 0.354684 2:-603586 None:intergenic
TTGATAGAGTTGATGCTGCT+TGG 0.384287 2:+600352 MsG0280006335.01.T01:intron
TCTTAGGTATAACAATAAAC+TGG 0.394588 2:-600795 None:intergenic
ATGTTACCTCTCCACATTGA+TGG 0.395745 2:-600567 None:intergenic
GAGCAGGATCCTATACTATA+TGG 0.401218 2:+601661 MsG0280006335.01.T01:CDS
TGATAGAGTTGATGCTGCTT+GGG 0.407596 2:+600353 MsG0280006335.01.T01:intron
GAACCATTTGTTTCATATGA+AGG 0.417481 2:+600670 MsG0280006335.01.T01:CDS
TCTGTATAGTTTCTCGCTGT+TGG 0.435505 2:+600741 MsG0280006335.01.T01:CDS
TTCCATCACGCGTCTCATCC+TGG 0.447960 2:+601639 MsG0280006335.01.T01:CDS
GTTGGTGCAGATGGGAAATC+AGG 0.452047 2:+601303 MsG0280006335.01.T01:CDS
ATAGTATAGGATCCTGCTCC+AGG 0.453668 2:-601657 None:intergenic
AATCATGACATGCTCAATCA+TGG 0.454257 2:-600482 None:intergenic
TCTCTTACACCAACTGTGTA+AGG 0.457899 2:-603448 None:intergenic
ATCCTATACTATATGGTGTA+AGG 0.459739 2:+601668 MsG0280006335.01.T01:CDS
CTCATCGATCGTTGATTCTA+GGG 0.460757 2:-603517 None:intergenic
GCACCACTATACAGTCCCTT+TGG 0.464450 2:-600541 None:intergenic
GTTTATTGTTATACCTAAGA+TGG 0.465619 2:+600798 MsG0280006335.01.T01:CDS
AGATCTATAGTTGGTGCAGA+TGG 0.465675 2:+601294 MsG0280006335.01.T01:intron
TTACCTTTCGCACTCCGTCT+TGG 0.468458 2:+601943 MsG0280006335.01.T01:CDS
CTGTGAAACTATTACATTCA+GGG 0.479207 2:-603217 None:intergenic
AGACCTTCATATGAAACAAA+TGG 0.484020 2:-600673 None:intergenic
CGAATTGATATGTACGGGTT+CGG 0.496092 2:-603676 None:intergenic
CACGCGTCTCATCCTGGAGC+AGG 0.500401 2:+601645 MsG0280006335.01.T01:CDS
TGTGTTTGTAGATCTATAGT+TGG 0.500413 2:+601285 MsG0280006335.01.T01:intron
CCTGAATGTAATAGTTTCAC+AGG 0.501576 2:+603218 MsG0280006335.01.T01:CDS
GACGAGATAACAGTGGTATC+CGG 0.506598 2:+603543 MsG0280006335.01.T01:CDS
GATGGGATCGCGATGTAAGA+AGG 0.529541 2:+603398 MsG0280006335.01.T01:intron
TTATGAAAATCATATGATAA+CGG 0.534435 2:-602104 None:intergenic
TTTCTTGGCTATGCATGCAG+TGG 0.534979 2:+599902 None:intergenic
TAAATATATGGTTAGAAGCA+TGG 0.536505 2:+602124 MsG0280006335.01.T01:CDS
TAGAATCAACGATCGATGAG+AGG 0.539794 2:+603520 MsG0280006335.01.T01:CDS
TACCTTACACCATATAGTAT+AGG 0.539849 2:-601670 None:intergenic
ATTCCAAAGGGACTGTATAG+TGG 0.539888 2:+600538 MsG0280006335.01.T01:CDS
TTAGAGATTCCTTACACAGT+TGG 0.545309 2:+603439 MsG0280006335.01.T01:CDS
AAGGTCTTGAGCAGCTGCAC+CGG 0.549157 2:+600689 MsG0280006335.01.T01:CDS
TTGTTACACAAAGCGCAAGA+TGG 0.553627 2:+600389 MsG0280006335.01.T01:CDS
AGCATGGAGGACGAATTTCA+TGG 0.554354 2:+602140 MsG0280006335.01.T01:CDS
TAGTGCGAATTGATATGTAC+GGG 0.554406 2:-603681 None:intergenic
ACAAAATTCTAACAGATACA+AGG 0.557031 2:-601403 None:intergenic
TCTCTGGAGAAAATTAAAGA+AGG 0.561099 2:+599957 MsG0280006335.01.T01:CDS
GGGAAGGTCTTGTTCAGCAC+AGG 0.563562 2:-600461 None:intergenic
GATCTATAGTTGGTGCAGAT+GGG 0.565228 2:+601295 MsG0280006335.01.T01:intron
AGAATCAACGATCGATGAGA+GGG 0.566055 2:+603521 MsG0280006335.01.T01:CDS
GATGAAACTACTATTCCAAA+GGG 0.569451 2:+600526 MsG0280006335.01.T01:CDS
ATGTACGGGTTCGGACAGAT+CGG 0.573862 2:-603667 None:intergenic
CAGTGGTTAAGAAGACAAGA+AGG 0.575007 2:+599919 MsG0280006335.01.T01:CDS
CTCCAGGATGAGACGCGTGA+TGG 0.577915 2:-601641 None:intergenic
AAACCAAGACGGAGTGCGAA+AGG 0.588150 2:-601946 None:intergenic
GCTACGGTGGCTCTTATCGA+TGG 0.590725 2:+601325 MsG0280006335.01.T01:CDS
ATCATGACATGCTCAATCAT+GGG 0.591999 2:-600481 None:intergenic
GTAGTGCGAATTGATATGTA+CGG 0.599536 2:-603682 None:intergenic
AGATGAAACTACTATTCCAA+AGG 0.604783 2:+600525 MsG0280006335.01.T01:CDS
GGTTAAGAAGACAAGAAGGA+AGG 0.621489 2:+599923 MsG0280006335.01.T01:CDS
GCAGATGGGAAATCAGGCTA+CGG 0.623298 2:+601309 MsG0280006335.01.T01:CDS
TGACATGCTCAATCATGGGA+AGG 0.634182 2:-600477 None:intergenic
CTTATCGATGGAAATTGGCA+AGG 0.636291 2:+601337 MsG0280006335.01.T01:CDS
AATTCTAACAGATACAAGGT+TGG 0.640730 2:-601399 None:intergenic
AGTTGTTCGAGTTAGCGTGC+CGG 0.646338 2:-603562 None:intergenic
TAAGAACACAAGTGATGAGG+CGG 0.651147 2:+601602 MsG0280006335.01.T01:CDS
TGTGAAACTATTACATTCAG+GGG 0.651741 2:-603216 None:intergenic
CAATGTTGTCAACGTAGAGG+AGG 0.653582 2:+603707 MsG0280006335.01.T01:CDS
GATGGGAAATCAGGCTACGG+TGG 0.662305 2:+601312 MsG0280006335.01.T01:CDS
AGGTAAGAACACAAGTGATG+AGG 0.668153 2:+601599 MsG0280006335.01.T01:intron
CTGTTGGTTAGCAATGAGCA+AGG 0.668181 2:+600757 MsG0280006335.01.T01:CDS
TGCACTCCATCAATGTGGAG+AGG 0.673997 2:+600561 MsG0280006335.01.T01:CDS
CAACAATGTTGTCAACGTAG+AGG 0.682543 2:+603704 MsG0280006335.01.T01:CDS
TTACAGTGATGAAACCAAGA+CGG 0.701613 2:-601957 None:intergenic
AGTGGTGCACTCCATCAATG+TGG 0.709568 2:+600556 MsG0280006335.01.T01:CDS
AGACGCGTGATGGAAAACAA+AGG 0.729624 2:-601631 None:intergenic
TGAGAGGGACGAGATAACAG+TGG 0.741797 2:+603536 MsG0280006335.01.T01:CDS
ATATATGGTTAGAAGCATGG+AGG 0.760811 2:+602127 MsG0280006335.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGATTTTCATAAATATA+TGG + Chr2:602112-602131 MsG0280006335.01.T01:CDS 10.0%
!! TATAAAATATTATATCAGTG+AGG - Chr2:602034-602053 None:intergenic 15.0%
!! TTATGAAAATCATATGATAA+CGG - Chr2:602107-602126 None:intergenic 15.0%
!!! TTTAATTGATTTTATTCTAC+AGG + Chr2:601579-601598 MsG0280006335.01.T01:intron 15.0%
!! AAAAACCAGATATATTGTTA+TGG + Chr2:602687-602706 MsG0280006335.01.T01:intron 20.0%
!! AAATTCTTAGAAAATCATGT+TGG + Chr2:602512-602531 MsG0280006335.01.T01:intron 20.0%
!! ACCTAAAATATGTAAGTATA+GGG - Chr2:601845-601864 None:intergenic 20.0%
!! ATTATTATACATACAATTGC+AGG - Chr2:602459-602478 None:intergenic 20.0%
!! TTATTATACATACAATTGCA+GGG - Chr2:602458-602477 None:intergenic 20.0%
!!! TTCTAAGAATTTCTTCATTT+TGG - Chr2:602504-602523 None:intergenic 20.0%
! AAAAACACTGCTATTCAAAA+GGG - Chr2:602188-602207 None:intergenic 25.0%
! AAAAAGGAAAATAACTGTGA+AGG - Chr2:602781-602800 None:intergenic 25.0%
! AAGCAGATTTAGAAGTAAAA+GGG - Chr2:601815-601834 None:intergenic 25.0%
! AATTGAGTTTCTAAATTTGC+AGG + Chr2:602402-602421 MsG0280006335.01.T01:intron 25.0%
! ACAAAATTCTAACAGATACA+AGG - Chr2:601406-601425 None:intergenic 25.0%
! ACTATAGTATAAATCCAAAG+CGG - Chr2:602288-602307 None:intergenic 25.0%
! ATGATCCATAACAATATATC+TGG - Chr2:602695-602714 None:intergenic 25.0%
! ATTACATTAGAAACAAACAG+TGG - Chr2:601451-601470 None:intergenic 25.0%
! CACATGTTCTATTATGAAAA+AGG - Chr2:602342-602361 None:intergenic 25.0%
! CACCTAAAATATGTAAGTAT+AGG - Chr2:601846-601865 None:intergenic 25.0%
! CATGAACAATAGAGAAAATA+AGG - Chr2:602806-602825 None:intergenic 25.0%
! GTTTATTGTTATACCTAAGA+TGG + Chr2:600798-600817 MsG0280006335.01.T01:CDS 25.0%
! TAAAAGCTTGTGATGTTAAT+AGG + Chr2:602558-602577 MsG0280006335.01.T01:intron 25.0%
! TAAAATATGTAAGTATAGGG+TGG - Chr2:601842-601861 None:intergenic 25.0%
! TAAATATATGGTTAGAAGCA+TGG + Chr2:602124-602143 MsG0280006335.01.T01:CDS 25.0%
! TAAGCAGATTTAGAAGTAAA+AGG - Chr2:601816-601835 None:intergenic 25.0%
! TAGAGAAAATAAGGACAAAA+AGG - Chr2:602797-602816 None:intergenic 25.0%
! TCTTAGGTATAACAATAAAC+TGG - Chr2:600798-600817 None:intergenic 25.0%
! TTCCTGAGAATTAAAAAGTA+GGG - Chr2:602075-602094 None:intergenic 25.0%
! TTTCATACACTACTATCAAA+TGG - Chr2:601111-601130 None:intergenic 25.0%
! TTTGATGGAAAACTGAAATA+AGG - Chr2:600274-600293 None:intergenic 25.0%
!! ACCCTATACTTACATATTTT+AGG + Chr2:601841-601860 MsG0280006335.01.T01:intron 25.0%
!! ATGTAAGAGCATATTCAATA+AGG + Chr2:600830-600849 MsG0280006335.01.T01:intron 25.0%
!! CTTATATATTTTCTACCCAA+TGG + Chr2:600892-600911 MsG0280006335.01.T01:intron 25.0%
!! GTGATATTTCAGGTAAAATA+TGG + Chr2:602722-602741 MsG0280006335.01.T01:intron 25.0%
!! TGTAAGAGCATATTCAATAA+GGG + Chr2:600831-600850 MsG0280006335.01.T01:intron 25.0%
!! TTTTCATCATAATCACACTA+TGG + Chr2:601474-601493 MsG0280006335.01.T01:intron 25.0%
!!! TATGCAATTGAAAAGTATTG+TGG - Chr2:603121-603140 None:intergenic 25.0%
!!! TTGAAAAGTATTGTGGAATA+TGG - Chr2:603114-603133 None:intergenic 25.0%
!!! TTTAGATCAAGTTTTATTGC+AGG - Chr2:600170-600189 None:intergenic 25.0%
AATTCTAACAGATACAAGGT+TGG - Chr2:601402-601421 None:intergenic 30.0%
ACCATTACATGATCATCATT+CGG + Chr2:603013-603032 MsG0280006335.01.T01:intron 30.0%
AGACCTTCATATGAAACAAA+TGG - Chr2:600676-600695 None:intergenic 30.0%
AGATGAAACTACTATTCCAA+AGG + Chr2:600525-600544 MsG0280006335.01.T01:CDS 30.0%
AGCTCAAAAACACAGAATTA+AGG + Chr2:600007-600026 MsG0280006335.01.T01:intron 30.0%
AGGAAGAAGAAATCAAAAGA+CGG - Chr2:602014-602033 None:intergenic 30.0%
ATAAGTATGATGTGACATTG+TGG + Chr2:601083-601102 MsG0280006335.01.T01:intron 30.0%
ATCCTATACTATATGGTGTA+AGG + Chr2:601668-601687 MsG0280006335.01.T01:CDS 30.0%
ATGAAAAAGGAAAGTTACCA+TGG - Chr2:602329-602348 None:intergenic 30.0%
ATTCAAAGACTCTAAGTCTA+AGG - Chr2:603589-603608 None:intergenic 30.0%
CAAAAACACTGCTATTCAAA+AGG - Chr2:602189-602208 None:intergenic 30.0%
CATACAGTTTGTGATATTTC+AGG + Chr2:602712-602731 MsG0280006335.01.T01:intron 30.0%
CATGCTTCAATTGTAAATGA+AGG + Chr2:600592-600611 MsG0280006335.01.T01:intron 30.0%
CTGTGAAACTATTACATTCA+GGG - Chr2:603220-603239 None:intergenic 30.0%
GAACCATTTGTTTCATATGA+AGG + Chr2:600670-600689 MsG0280006335.01.T01:CDS 30.0%
GAAGTAGCTTAATTAAGCAA+TGG + Chr2:600955-600974 MsG0280006335.01.T01:intron 30.0%
GATGAAACTACTATTCCAAA+GGG + Chr2:600526-600545 MsG0280006335.01.T01:CDS 30.0%
GATTGTTAGAAAAATTCAGC+TGG - Chr2:603635-603654 None:intergenic 30.0%
GCAAGTAATGAAACAAATGT+AGG - Chr2:602901-602920 None:intergenic 30.0%
GCTAGTAAATACTCAAGTAA+AGG - Chr2:601206-601225 None:intergenic 30.0%
GTTCCTGAGAATTAAAAAGT+AGG - Chr2:602076-602095 None:intergenic 30.0%
TAAAGTTGCCAAAGATTTGA+TGG - Chr2:600289-600308 None:intergenic 30.0%
TAAGTATGATGTGACATTGT+GGG + Chr2:601084-601103 MsG0280006335.01.T01:intron 30.0%
TACCTTACACCATATAGTAT+AGG - Chr2:601673-601692 None:intergenic 30.0%
TACTTGCATTAATCTGCTTA+AGG + Chr2:602914-602933 MsG0280006335.01.T01:intron 30.0%
TGATTATTGAATGTTCACAC+TGG - Chr2:600243-600262 None:intergenic 30.0%
TGCAAACAATAAGTAAGGAT+GGG - Chr2:601716-601735 None:intergenic 30.0%
TGTGAAACTATTACATTCAG+GGG - Chr2:603219-603238 None:intergenic 30.0%
TGTGTTTGTAGATCTATAGT+TGG + Chr2:601285-601304 MsG0280006335.01.T01:intron 30.0%
TTGCAAACAATAAGTAAGGA+TGG - Chr2:601717-601736 None:intergenic 30.0%
TTGCTTGCAAACAATAAGTA+AGG - Chr2:601721-601740 None:intergenic 30.0%
TTTGTATGTCTATGTCTAGT+GGG + Chr2:602960-602979 MsG0280006335.01.T01:intron 30.0%
! ATATATTTTCTACCCAATGG+AGG + Chr2:600895-600914 MsG0280006335.01.T01:intron 30.0%
! TCAGTTTTCCATCAAATCTT+TGG + Chr2:600278-600297 MsG0280006335.01.T01:intron 30.0%
! TCTCTGGAGAAAATTAAAGA+AGG + Chr2:599957-599976 MsG0280006335.01.T01:CDS 30.0%
! TTTTGTATGTCTATGTCTAG+TGG + Chr2:602959-602978 MsG0280006335.01.T01:intron 30.0%
!! ATTAAATGCTGTTTCTTTGC+AGG + Chr2:600642-600661 MsG0280006335.01.T01:intron 30.0%
!! TTAAATGCTGTTTCTTTGCA+GGG + Chr2:600643-600662 MsG0280006335.01.T01:intron 30.0%
AACACAGAATTAAGGACACT+AGG + Chr2:600015-600034 MsG0280006335.01.T01:intron 35.0%
AACCACTGCTTACTTAACAT+AGG - Chr2:601249-601268 None:intergenic 35.0%
AATCATGACATGCTCAATCA+TGG - Chr2:600485-600504 None:intergenic 35.0%
ACACAGAATTAAGGACACTA+GGG + Chr2:600016-600035 MsG0280006335.01.T01:intron 35.0%
ACATACAATTGCAGGGATAA+AGG - Chr2:602451-602470 None:intergenic 35.0%
ACATGAACATTGACCATCTT+AGG - Chr2:600814-600833 None:intergenic 35.0%
ACCGAATGATGATCATGTAA+TGG - Chr2:603017-603036 None:intergenic 35.0%
ACTGCTTACTTAACATAGGA+AGG - Chr2:601245-601264 None:intergenic 35.0%
ATCATGACATGCTCAATCAT+GGG - Chr2:600484-600503 None:intergenic 35.0%
CAGGTCTGAATATATACTTC+TGG + Chr2:602421-602440 MsG0280006335.01.T01:intron 35.0%
CCTGAATGTAATAGTTTCAC+AGG + Chr2:603218-603237 MsG0280006335.01.T01:CDS 35.0%
CCTGTGAAACTATTACATTC+AGG - Chr2:603221-603240 None:intergenic 35.0%
GTAGTGCGAATTGATATGTA+CGG - Chr2:603685-603704 None:intergenic 35.0%
TACCAATGTCCATATGAATG+AGG - Chr2:602848-602867 None:intergenic 35.0%
TAGTGCGAATTGATATGTAC+GGG - Chr2:603684-603703 None:intergenic 35.0%
TATGAATGAGGCCATTTCAA+AGG - Chr2:602836-602855 None:intergenic 35.0%
TCCCGAGAACAAATAAAAAC+CGG - Chr2:600711-600730 None:intergenic 35.0%
TTACAGTGATGAAACCAAGA+CGG - Chr2:601960-601979 None:intergenic 35.0%
TTAGAGATTCCTTACACAGT+TGG + Chr2:603439-603458 MsG0280006335.01.T01:CDS 35.0%
TTCCTATGTTAAGTAAGCAG+TGG + Chr2:601244-601263 MsG0280006335.01.T01:intron 35.0%
TTGAAATGGCCTCATTCATA+TGG + Chr2:602836-602855 MsG0280006335.01.T01:intron 35.0%
TTGTATGTCTATGTCTAGTG+GGG + Chr2:602961-602980 MsG0280006335.01.T01:intron 35.0%
TTTACTTGTGTTCTTGCACT+TGG - Chr2:602642-602661 None:intergenic 35.0%
! AAGTTAGCAGTGTCTAAGAT+TGG + Chr2:600132-600151 MsG0280006335.01.T01:intron 35.0%
! AGAATTTTGTCAATGCCTGA+AGG + Chr2:601416-601435 MsG0280006335.01.T01:CDS 35.0%
! AGTACTTAGAGCATCTCTAA+TGG - Chr2:601161-601180 None:intergenic 35.0%
! ATATATGGTTAGAAGCATGG+AGG + Chr2:602127-602146 MsG0280006335.01.T01:CDS 35.0%
! CACTCTTGTTTACTACAGAT+GGG + Chr2:603381-603400 MsG0280006335.01.T01:intron 35.0%
! GAATTTTGTCAATGCCTGAA+GGG + Chr2:601417-601436 MsG0280006335.01.T01:CDS 35.0%
! TATGTTTTCTGCGTCAGTTT+AGG + Chr2:601899-601918 MsG0280006335.01.T01:intron 35.0%
! TCACTCTTGTTTACTACAGA+TGG + Chr2:603380-603399 MsG0280006335.01.T01:intron 35.0%
! TTCATGGCATTCTAGGAATA+AGG + Chr2:602156-602175 MsG0280006335.01.T01:CDS 35.0%
!! ACCGGTTTTTATTTGTTCTC+GGG + Chr2:600707-600726 MsG0280006335.01.T01:CDS 35.0%
!! TTCTGTGTTTTTGAGCTAGT+TGG - Chr2:600004-600023 None:intergenic 35.0%
!!! CACCGGTTTTTATTTGTTCT+CGG + Chr2:600706-600725 MsG0280006335.01.T01:CDS 35.0%
!!! CTCCCTACTTTTTAATTCTC+AGG + Chr2:602070-602089 MsG0280006335.01.T01:intron 35.0%
ACTAAGTTTGATCGGCTACA+TGG - Chr2:601511-601530 None:intergenic 40.0%
AGAATCAACGATCGATGAGA+GGG + Chr2:603521-603540 MsG0280006335.01.T01:CDS 40.0%
AGGTAAGAACACAAGTGATG+AGG + Chr2:601599-601618 MsG0280006335.01.T01:intron 40.0%
ATCATCATTCGGTTGACATC+TGG + Chr2:603024-603043 MsG0280006335.01.T01:intron 40.0%
ATGTAGCCGATCAAACTTAG+TGG + Chr2:601510-601529 MsG0280006335.01.T01:intron 40.0%
ATGTTACCTCTCCACATTGA+TGG - Chr2:600570-600589 None:intergenic 40.0%
ATTCCAAAGGGACTGTATAG+TGG + Chr2:600538-600557 MsG0280006335.01.T01:CDS 40.0%
CAACAATGTTGTCAACGTAG+AGG + Chr2:603704-603723 MsG0280006335.01.T01:CDS 40.0%
CAGCATCAACTCTATCAACA+AGG - Chr2:600350-600369 None:intergenic 40.0%
CAGTGGTTAAGAAGACAAGA+AGG + Chr2:599919-599938 MsG0280006335.01.T01:CDS 40.0%
CCAGATTTCAAGCGCTAAAA+TGG - Chr2:602607-602626 None:intergenic 40.0%
CCTATCCCACTAAGTTTGAT+CGG - Chr2:601519-601538 None:intergenic 40.0%
CGAATTGATATGTACGGGTT+CGG - Chr2:603679-603698 None:intergenic 40.0%
CTCAAGCACATAAAGCATAG+TGG - Chr2:600053-600072 None:intergenic 40.0%
CTTATCGATGGAAATTGGCA+AGG + Chr2:601337-601356 MsG0280006335.01.T01:CDS 40.0%
GAAAACATAGCTGCAATGCA+AGG - Chr2:601888-601907 None:intergenic 40.0%
GACGAATTTCATGGCATTCT+AGG + Chr2:602149-602168 MsG0280006335.01.T01:CDS 40.0%
GAGCAGGATCCTATACTATA+TGG + Chr2:601661-601680 MsG0280006335.01.T01:CDS 40.0%
GGTTAAGAAGACAAGAAGGA+AGG + Chr2:599923-599942 MsG0280006335.01.T01:CDS 40.0%
TAAGAACACAAGTGATGAGG+CGG + Chr2:601602-601621 MsG0280006335.01.T01:CDS 40.0%
TAAGGACACTAGGGTGATTA+TGG + Chr2:600025-600044 MsG0280006335.01.T01:intron 40.0%
TAGAATCAACGATCGATGAG+AGG + Chr2:603520-603539 MsG0280006335.01.T01:CDS 40.0%
TCATCATTCGGTTGACATCT+GGG + Chr2:603025-603044 MsG0280006335.01.T01:intron 40.0%
TCTCTTACACCAACTGTGTA+AGG - Chr2:603451-603470 None:intergenic 40.0%
TCTGTATAGTTTCTCGCTGT+TGG + Chr2:600741-600760 MsG0280006335.01.T01:CDS 40.0%
TGAGTGAAGATTAGAGAGTG+AGG + Chr2:600095-600114 MsG0280006335.01.T01:intron 40.0%
TGCATCTTTGTGAACATCCA+TGG + Chr2:602309-602328 MsG0280006335.01.T01:intron 40.0%
TGGCTCTTATCGATGGAAAT+TGG + Chr2:601332-601351 MsG0280006335.01.T01:CDS 40.0%
TGTAGCCGATCAAACTTAGT+GGG + Chr2:601511-601530 MsG0280006335.01.T01:intron 40.0%
TTGTTACACAAAGCGCAAGA+TGG + Chr2:600389-600408 MsG0280006335.01.T01:CDS 40.0%
! AGACACTGCTAACTTTTCAG+TGG - Chr2:600127-600146 None:intergenic 40.0%
! AGATCTATAGTTGGTGCAGA+TGG + Chr2:601294-601313 MsG0280006335.01.T01:intron 40.0%
! CAGTGGAAATTTTTCCCTTC+AGG - Chr2:601434-601453 None:intergenic 40.0%
! CATGCAGAATGCCTTTGAAA+TGG + Chr2:602822-602841 MsG0280006335.01.T01:intron 40.0%
! GATCTATAGTTGGTGCAGAT+GGG + Chr2:601295-601314 MsG0280006335.01.T01:intron 40.0%
!! CCATTTTAGCGCTTGAAATC+TGG + Chr2:602604-602623 MsG0280006335.01.T01:intron 40.0%
!! CTCATCGATCGTTGATTCTA+GGG - Chr2:603520-603539 None:intergenic 40.0%
!! GTGTTTTTGAGCTAGTTGGT+AGG - Chr2:600000-600019 None:intergenic 40.0%
!! TCTCATCGATCGTTGATTCT+AGG - Chr2:603521-603540 None:intergenic 40.0%
!! TGATAGAGTTGATGCTGCTT+GGG + Chr2:600353-600372 MsG0280006335.01.T01:intron 40.0%
!! TTGATAGAGTTGATGCTGCT+TGG + Chr2:600352-600371 MsG0280006335.01.T01:intron 40.0%
!!! GCAGTGGTTTAGTTTTTCTG+TGG + Chr2:601260-601279 MsG0280006335.01.T01:intron 40.0%
ACACCTCAAGTTCCTCCATT+GGG - Chr2:600910-600929 None:intergenic 45.0%
AGCATGGAGGACGAATTTCA+TGG + Chr2:602140-602159 MsG0280006335.01.T01:CDS 45.0%
ATAGTATAGGATCCTGCTCC+AGG - Chr2:601660-601679 None:intergenic 45.0%
CAAACTTAGTGGGATAGGCT+TGG + Chr2:601521-601540 MsG0280006335.01.T01:intron 45.0%
CAATGTTGTCAACGTAGAGG+AGG + Chr2:603707-603726 MsG0280006335.01.T01:CDS 45.0%
CCGATCAAACTTAGTGGGAT+AGG + Chr2:601516-601535 MsG0280006335.01.T01:intron 45.0%
GAACAAGAACACTGCAATGC+AGG - Chr2:603170-603189 None:intergenic 45.0%
GACGAGATAACAGTGGTATC+CGG + Chr2:603543-603562 MsG0280006335.01.T01:CDS 45.0%
GCAGTGTTCTTGTTCAGTTC+TGG + Chr2:603175-603194 MsG0280006335.01.T01:intron 45.0%
GGCCTCATTCATATGGACAT+TGG + Chr2:602843-602862 MsG0280006335.01.T01:intron 45.0%
TGACATGCTCAATCATGGGA+AGG - Chr2:600480-600499 None:intergenic 45.0%
! AGACGCGTGATGGAAAACAA+AGG - Chr2:601634-601653 None:intergenic 45.0%
! CTGTTGGTTAGCAATGAGCA+AGG + Chr2:600757-600776 MsG0280006335.01.T01:CDS 45.0%
!!! GAAGGCACTTTTTGCTTCTC+TGG + Chr2:599941-599960 MsG0280006335.01.T01:CDS 45.0%
!! TTTATTATATATAATTAACT+TGG + Chr2:600318-600337 MsG0280006335.01.T01:intron 5.0%
AAACCAAGACGGAGTGCGAA+AGG - Chr2:601949-601968 None:intergenic 50.0%
ACCTCCTTGAACTTTGCAGC+TGG + Chr2:602262-602281 MsG0280006335.01.T01:intron 50.0%
AGTGGTGCACTCCATCAATG+TGG + Chr2:600556-600575 MsG0280006335.01.T01:CDS 50.0%
ATGTACGGGTTCGGACAGAT+CGG - Chr2:603670-603689 None:intergenic 50.0%
CTACCCAATGGAGGAACTTG+AGG + Chr2:600904-600923 MsG0280006335.01.T01:intron 50.0%
CTTGAAATCTGGCAGCAGCA+GGG + Chr2:602615-602634 MsG0280006335.01.T01:intron 50.0%
GAACCAAAAAGACCATCGCG+CGG - Chr2:602666-602685 None:intergenic 50.0%
GACACCTCAAGTTCCTCCAT+TGG - Chr2:600911-600930 None:intergenic 50.0%
GATGGGATCGCGATGTAAGA+AGG + Chr2:603398-603417 MsG0280006335.01.T01:intron 50.0%
GCACCACTATACAGTCCCTT+TGG - Chr2:600544-600563 None:intergenic 50.0%
GCAGATGGGAAATCAGGCTA+CGG + Chr2:601309-601328 MsG0280006335.01.T01:CDS 50.0%
GTTGGTGCAGATGGGAAATC+AGG + Chr2:601303-601322 MsG0280006335.01.T01:CDS 50.0%
TGAGAGGGACGAGATAACAG+TGG + Chr2:603536-603555 MsG0280006335.01.T01:CDS 50.0%
TGCACTCCATCAATGTGGAG+AGG + Chr2:600561-600580 MsG0280006335.01.T01:CDS 50.0%
TTACCTTTCGCACTCCGTCT+TGG + Chr2:601943-601962 MsG0280006335.01.T01:CDS 50.0%
! AGTTGTTCGAGTTAGCGTGC+CGG - Chr2:603565-603584 None:intergenic 50.0%
AACTTTGCAGCTGGCCGCTT+TGG + Chr2:602271-602290 MsG0280006335.01.T01:intron 55.0%
AAGGTCTTGAGCAGCTGCAC+CGG + Chr2:600689-600708 MsG0280006335.01.T01:CDS 55.0%
ACACAAGTAAAGCCGCGCGA+TGG + Chr2:602651-602670 MsG0280006335.01.T01:intron 55.0%
GATGGGAAATCAGGCTACGG+TGG + Chr2:601312-601331 MsG0280006335.01.T01:CDS 55.0%
GCCAGCTGCAAAGTTCAAGG+AGG - Chr2:602266-602285 None:intergenic 55.0%
GCTACGGTGGCTCTTATCGA+TGG + Chr2:601325-601344 MsG0280006335.01.T01:CDS 55.0%
GCTTGAAATCTGGCAGCAGC+AGG + Chr2:602614-602633 MsG0280006335.01.T01:intron 55.0%
TTCCATCACGCGTCTCATCC+TGG + Chr2:601639-601658 MsG0280006335.01.T01:CDS 55.0%
! AAGCCGCGCGATGGTCTTTT+TGG + Chr2:602660-602679 MsG0280006335.01.T01:intron 55.0%
!! GGGAAGGTCTTGTTCAGCAC+AGG - Chr2:600464-600483 None:intergenic 55.0%
CTCCAGGATGAGACGCGTGA+TGG - Chr2:601644-601663 None:intergenic 60.0%
GCGGCCAGCTGCAAAGTTCA+AGG - Chr2:602269-602288 None:intergenic 60.0%
CACGCGTCTCATCCTGGAGC+AGG + Chr2:601645-601664 MsG0280006335.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 599912 603770 599912 ID=MsG0280006335.01;Name=MsG0280006335.01
Chr2 mRNA 599912 603770 599912 ID=MsG0280006335.01.T01;Parent=MsG0280006335.01;Name=MsG0280006335.01.T01;_AED=0.49;_eAED=0.56;_QI=0|0|0|1|0.5|0.66|9|0|427
Chr2 exon 599912 599978 599912 ID=MsG0280006335.01.T01:exon:667;Parent=MsG0280006335.01.T01
Chr2 exon 600359 600582 600359 ID=MsG0280006335.01.T01:exon:668;Parent=MsG0280006335.01.T01
Chr2 exon 600664 600831 600664 ID=MsG0280006335.01.T01:exon:669;Parent=MsG0280006335.01.T01
Chr2 exon 601296 601452 601296 ID=MsG0280006335.01.T01:exon:670;Parent=MsG0280006335.01.T01
Chr2 exon 601601 601689 601601 ID=MsG0280006335.01.T01:exon:671;Parent=MsG0280006335.01.T01
Chr2 exon 601916 601975 601916 ID=MsG0280006335.01.T01:exon:672;Parent=MsG0280006335.01.T01
Chr2 exon 602092 602177 602092 ID=MsG0280006335.01.T01:exon:673;Parent=MsG0280006335.01.T01
Chr2 exon 603179 603239 603179 ID=MsG0280006335.01.T01:exon:674;Parent=MsG0280006335.01.T01
Chr2 exon 603399 603770 603399 ID=MsG0280006335.01.T01:exon:675;Parent=MsG0280006335.01.T01
Chr2 CDS 599912 599978 599912 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 600359 600582 600359 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 600664 600831 600664 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 601296 601452 601296 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 601601 601689 601601 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 601916 601975 601916 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 602092 602177 602092 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 603179 603239 603179 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Chr2 CDS 603399 603770 603399 ID=MsG0280006335.01.T01:cds;Parent=MsG0280006335.01.T01
Gene Sequence

>MsG0280006335.01.T01

ATGCATGCAGTGGTTAAGAAGACAAGAAGGAAGGCACTTTTTGCTTCTCTGGAGAAAATTAAAGAAGAGTTGATGCTGCTTGGGCTTATCTCTTTGTTTGTTACACAAAGCGCAAGATGGATCTCTGAAATTTGTGTAAATTCATCACTTTTCAGCAGTCGATTCTATACCTGTGCTGAACAAGACCTTCCCATGATTGAGCATGTCATGATTGTAAACTCTTCATCTTTCTCAGATGAAACTACTATTCCAAAGGGACTGTATAGTGGTGCACTCCATCAATGTGGAGAGGGTCATGAACCATTTGTTTCATATGAAGGTCTTGAGCAGCTGCACCGGTTTTTATTTGTTCTCGGGATCACTCATGTTCTGTATAGTTTCTCGCTGTTGGTTAGCAATGAGCAAGGTCAGTCATTAAATTTCCAGTTTATTGTTATACCTAAGATGGTCAATGTTCATATCTATAGTTGGTGCAGATGGGAAATCAGGCTACGGTGGCTCTTATCGATGGAAATTGGCAAGGTAAACTACATAGAAATTGAATGTTGCTTCATTGATGACACCAACCTTGTATCTGTTAGAATTTTGTCAATGCCTGAAGGGAAAAATTTCCACTGTAAGAACACAAGTGATGAGGCGGCAAACACCTTTGTTTTCCATCACGCGTCTCATCCTGGAGCAGGATCCTATACTATATGGTGTAAGTTTAGGAGTTCTATACAAAAATCGAGATTACCTTTCGCACTCCGTCTTGGTTTCATCACTGAACATAAATTGCCGTTATCATATGATTTTCATAAATATATGGTTAGAAGCATGGAGGACGAATTTCATGGCATTCTAGGAATAAGTGTTCTTGTTCAGTTCTGGTGTAGCTATATGACAGTACCCCTGAATGTAATAGTTTCACAGATGGGATCGCGATGTAAGAAGGCTCTAGTGACAGAGAGTGTTAGAGATTCCTTACACAGTTGGTGTAAGAGAGTAAAACAGAAGTCTAAGCATTCACATACTGCTAGATCAATATGTTCCCTAGAATCAACGATCGATGAGAGGGACGAGATAACAGTGGTATCCGGCACGCTAACTCGAACAACTTCCTTAGACTTAGAGTCTTTGAATCAGATAACAGTAACATCAGTAGACCAGCTGAATTTTTCTAACAATCTTCAAGATTCAACCGATCTGTCCGAACCCGTACATATCAATTCGCACTACAACAATGTTGTCAACGTAGAGGAGGCTAAAGTTGAAACACTTCTTGATTTGTTTCACAAAACATGA
Protein sequence

>MsG0280006335.01.T01

MHAVVKKTRRKALFASLEKIKEELMLLGLISLFVTQSARWISEICVNSSLFSSRFYTCAEQDLPMIEHVMIVNSSSFSDETTIPKGLYSGALHQCGEGHEPFVSYEGLEQLHRFLFVLGITHVLYSFSLLVSNEQGQSLNFQFIVIPKMVNVHIYSWCRWEIRLRWLLSMEIGKVNYIEIECCFIDDTNLVSVRILSMPEGKNFHCKNTSDEAANTFVFHHASHPGAGSYTIWCKFRSSIQKSRLPFALRLGFITEHKLPLSYDFHKYMVRSMEDEFHGILGISVLVQFWCSYMTVPLNVIVSQMGSRCKKALVTESVRDSLHSWCKRVKQKSKHSHTARSICSLESTIDERDEITVVSGTLTRTTSLDLESLNQITVTSVDQLNFSNNLQDSTDLSEPVHINSHYNNVVNVEEAKVETLLDLFHKT*