Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006773.01.T01 | XP_003593648.1 | 97.156 | 211 | 5 | 1 | 1 | 211 | 1 | 210 | 3.08E-147 | 418 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006773.01.T01 | Q9FNL3 | 61.682 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 1.41E-96 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006773.01.T01 | A2Q514 | 97.156 | 211 | 5 | 1 | 1 | 211 | 1 | 210 | 1.47e-147 | 418 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280006312.01 | MsG0280006773.01 | PPI |
| MsG0280006773.01 | MsG0280006312.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006773.01.T01 | MTR_2g014550 | 97.156 | 211 | 5 | 1 | 1 | 211 | 1 | 210 | 3.73e-151 | 418 |
| MsG0280006773.01.T01 | MTR_2g014520 | 87.440 | 207 | 26 | 0 | 5 | 211 | 1 | 207 | 3.10e-134 | 375 |
| MsG0280006773.01.T01 | MTR_4g088335 | 73.604 | 197 | 52 | 0 | 15 | 211 | 10 | 206 | 1.55e-109 | 313 |
| MsG0280006773.01.T01 | MTR_4g078870 | 55.440 | 193 | 80 | 3 | 20 | 209 | 14 | 203 | 5.36e-76 | 228 |
| MsG0280006773.01.T01 | MTR_5g044580 | 43.094 | 181 | 101 | 2 | 26 | 205 | 35 | 214 | 5.61e-49 | 159 |
| MsG0280006773.01.T01 | MTR_2g020850 | 39.474 | 190 | 108 | 2 | 25 | 210 | 2 | 188 | 1.21e-46 | 152 |
| MsG0280006773.01.T01 | MTR_7g010890 | 41.436 | 181 | 104 | 2 | 26 | 205 | 35 | 214 | 5.46e-46 | 152 |
| MsG0280006773.01.T01 | MTR_4g118470 | 41.361 | 191 | 98 | 3 | 24 | 210 | 18 | 198 | 4.26e-43 | 144 |
| MsG0280006773.01.T01 | MTR_4g118480 | 42.045 | 176 | 92 | 2 | 39 | 210 | 27 | 196 | 1.19e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006773.01.T01 | AT5G46090 | 61.682 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 1.44e-97 | 283 |
| MsG0280006773.01.T01 | AT4G18425 | 59.434 | 212 | 82 | 3 | 3 | 210 | 1 | 212 | 3.86e-92 | 269 |
| MsG0280006773.01.T01 | AT4G28485 | 52.459 | 183 | 86 | 1 | 28 | 209 | 16 | 198 | 8.61e-67 | 204 |
| MsG0280006773.01.T01 | AT3G02430 | 47.917 | 192 | 94 | 2 | 24 | 211 | 26 | 215 | 5.95e-65 | 200 |
| MsG0280006773.01.T01 | AT4G24310 | 50.000 | 192 | 89 | 3 | 23 | 209 | 18 | 207 | 5.18e-61 | 190 |
| MsG0280006773.01.T01 | AT1G09157 | 38.587 | 184 | 101 | 1 | 34 | 205 | 56 | 239 | 1.67e-42 | 144 |
| MsG0280006773.01.T01 | AT3G21550 | 43.605 | 172 | 91 | 2 | 37 | 207 | 6 | 172 | 5.22e-42 | 140 |
| MsG0280006773.01.T01 | AT5G39650 | 38.172 | 186 | 99 | 2 | 34 | 205 | 57 | 240 | 6.30e-42 | 142 |
| MsG0280006773.01.T01 | AT3G21520 | 40.000 | 160 | 89 | 2 | 49 | 206 | 33 | 187 | 6.04e-36 | 125 |
| MsG0280006773.01.T01 | AT4G28485 | 56.566 | 99 | 43 | 0 | 111 | 209 | 65 | 163 | 1.67e-33 | 118 |
| MsG0280006773.01.T01 | AT5G27370 | 35.227 | 176 | 99 | 3 | 45 | 205 | 11 | 186 | 2.63e-30 | 110 |
Find 43 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCAGAGCACGGCGCATT+TGG | 0.350269 | 2:-6022360 | MsG0280006773.01.T01:CDS |
| AGCAAGGGGAATATTTGTTA+TGG | 0.356880 | 2:-6022173 | MsG0280006773.01.T01:CDS |
| AAAGGTTTGTGGGTCATTGA+TGG | 0.367892 | 2:-6022137 | MsG0280006773.01.T01:CDS |
| GCAAGGGGAATATTTGTTAT+GGG | 0.374957 | 2:-6022172 | MsG0280006773.01.T01:CDS |
| ATTAACTAACTTGTTGAAAG+AGG | 0.380446 | 2:+6021867 | None:intergenic |
| ACGGCGTTGCCTGTGGCAAT+TGG | 0.380489 | 2:-6021948 | MsG0280006773.01.T01:CDS |
| GGTTCCACTTCTGCGGAATT+CGG | 0.390562 | 2:-6022432 | MsG0280006773.01.T01:CDS |
| CCTACTGAGAGACATGGAAT+TGG | 0.411080 | 2:-6021894 | MsG0280006773.01.T01:CDS |
| ACCAGGCTCCTCTCTGCCTC+TGG | 0.411547 | 2:+6022404 | None:intergenic |
| TATGGGTTTGCCACCTTCAA+AGG | 0.413754 | 2:-6022155 | MsG0280006773.01.T01:CDS |
| TGCCACCTTCAAAGGTTTGT+GGG | 0.416898 | 2:-6022147 | MsG0280006773.01.T01:CDS |
| AGCAACTTGAGGTGGAAGTT+TGG | 0.423290 | 2:+6022109 | None:intergenic |
| AACTTATAGCTTTCTGTACC+AGG | 0.430636 | 2:+6022387 | None:intergenic |
| GCATGCGGTGATGTCAATTC+TGG | 0.434994 | 2:-6022054 | MsG0280006773.01.T01:CDS |
| GAACAGTGCCTGTAGGTAGA+AGG | 0.449758 | 2:+6022333 | None:intergenic |
| AATGACCCACAAACCTTTGA+AGG | 0.450179 | 2:+6022142 | None:intergenic |
| TTGCCACCTTCAAAGGTTTG+TGG | 0.452480 | 2:-6022148 | MsG0280006773.01.T01:CDS |
| TGCTATCAGTAAAGTTTAGC+AGG | 0.458079 | 2:+6022204 | None:intergenic |
| TAGGAATGACGATGAACAAA+AGG | 0.495588 | 2:-6022453 | MsG0280006773.01.T01:CDS |
| TTAACTAACTTGTTGAAAGA+GGG | 0.526967 | 2:+6021868 | None:intergenic |
| TTGGCAAACCTTCTACCTAC+AGG | 0.528525 | 2:-6022341 | MsG0280006773.01.T01:CDS |
| TCTGTACTTGTGACTCTATG+TGG | 0.546587 | 2:-6022239 | MsG0280006773.01.T01:CDS |
| TCCAGAGGCAGAGAGGAGCC+TGG | 0.549208 | 2:-6022405 | MsG0280006773.01.T01:CDS |
| CCAATTCCATGTCTCTCAGT+AGG | 0.555703 | 2:+6021894 | None:intergenic |
| TAGAAATCGATGATGACTCT+AGG | 0.560367 | 2:-6022472 | MsG0280006773.01.T01:CDS |
| AGTCACAAGTACAGAAGTCA+TGG | 0.564650 | 2:+6022247 | None:intergenic |
| ACCTCCGAATTCCGCAGAAG+TGG | 0.566239 | 2:+6022428 | None:intergenic |
| TCCACTTCTGCGGAATTCGG+AGG | 0.571752 | 2:-6022429 | MsG0280006773.01.T01:CDS |
| TGATAGCATTAGAGACAGCA+AGG | 0.586072 | 2:-6022189 | MsG0280006773.01.T01:CDS |
| GTTTATAGATTTCATGCATG+CGG | 0.586781 | 2:-6022069 | MsG0280006773.01.T01:CDS |
| AACAAAAGGTTCCACTTCTG+CGG | 0.591604 | 2:-6022439 | MsG0280006773.01.T01:CDS |
| AAGTTCGACATTTCAGAGCA+CGG | 0.595642 | 2:-6022369 | MsG0280006773.01.T01:CDS |
| AATCCTCACGGCGTTGCCTG+TGG | 0.613810 | 2:-6021955 | MsG0280006773.01.T01:CDS |
| TTGCCACAGGCAACGCCGTG+AGG | 0.614771 | 2:+6021952 | None:intergenic |
| GCGGAATTCGGAGGTTCCAG+AGG | 0.623166 | 2:-6022420 | MsG0280006773.01.T01:CDS |
| TGCATTGTTTGATCAGAATG+TGG | 0.627363 | 2:-6022018 | MsG0280006773.01.T01:CDS |
| CGGAGGTTCCAGAGGCAGAG+AGG | 0.628788 | 2:-6022412 | MsG0280006773.01.T01:CDS |
| GCATTTCCTACTGAGAGACA+TGG | 0.635534 | 2:-6021900 | MsG0280006773.01.T01:CDS |
| GATAGCATTAGAGACAGCAA+GGG | 0.637719 | 2:-6022188 | MsG0280006773.01.T01:CDS |
| GACCCACAAACCTTTGAAGG+TGG | 0.639111 | 2:+6022145 | None:intergenic |
| AATGCAAGAACAGTGCCTGT+AGG | 0.674988 | 2:+6022326 | None:intergenic |
| AGAGATACAAGAAATCCTCA+CGG | 0.689697 | 2:-6021967 | MsG0280006773.01.T01:CDS |
| ATAGCATTAGAGACAGCAAG+GGG | 0.732552 | 2:-6022187 | MsG0280006773.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGCCAACATTTGTAAAAAT+TGG | + | Chr2:6022070-6022089 | None:intergenic | 25.0% |
| !! | ATTTCTTGTATCTCTTTTGA+TGG | + | Chr2:6022385-6022404 | None:intergenic | 25.0% |
| !! | TAAAAATTGGTGACAAAAGT+TGG | + | Chr2:6022057-6022076 | None:intergenic | 25.0% |
| !! | TCACCAATTTTTACAAATGT+TGG | - | Chr2:6022064-6022083 | MsG0280006773.01.T01:CDS | 25.0% |
| GTTTATAGATTTCATGCATG+CGG | - | Chr2:6022288-6022307 | MsG0280006773.01.T01:CDS | 30.0% | |
| AACTTATAGCTTTCTGTACC+AGG | + | Chr2:6021973-6021992 | None:intergenic | 35.0% | |
| AGAGATACAAGAAATCCTCA+CGG | - | Chr2:6022390-6022409 | MsG0280006773.01.T01:CDS | 35.0% | |
| TAGAAATCGATGATGACTCT+AGG | - | Chr2:6021885-6021904 | MsG0280006773.01.T01:CDS | 35.0% | |
| TAGGAATGACGATGAACAAA+AGG | - | Chr2:6021904-6021923 | MsG0280006773.01.T01:CDS | 35.0% | |
| TGCATTGTTTGATCAGAATG+TGG | - | Chr2:6022339-6022358 | MsG0280006773.01.T01:CDS | 35.0% | |
| TGCTATCAGTAAAGTTTAGC+AGG | + | Chr2:6022156-6022175 | None:intergenic | 35.0% | |
| ! | AGCAAGGGGAATATTTGTTA+TGG | - | Chr2:6022184-6022203 | MsG0280006773.01.T01:CDS | 35.0% |
| !! | GCAAGGGGAATATTTGTTAT+GGG | - | Chr2:6022185-6022204 | MsG0280006773.01.T01:CDS | 35.0% |
| !! | TGTATCTCTTTTGATGGTTC+TGG | + | Chr2:6022379-6022398 | None:intergenic | 35.0% |
| AAAGGTTTGTGGGTCATTGA+TGG | - | Chr2:6022220-6022239 | MsG0280006773.01.T01:CDS | 40.0% | |
| AACAAAAGGTTCCACTTCTG+CGG | - | Chr2:6021918-6021937 | MsG0280006773.01.T01:CDS | 40.0% | |
| AAGTTCGACATTTCAGAGCA+CGG | - | Chr2:6021988-6022007 | MsG0280006773.01.T01:CDS | 40.0% | |
| AATGACCCACAAACCTTTGA+AGG | + | Chr2:6022218-6022237 | None:intergenic | 40.0% | |
| AGTCACAAGTACAGAAGTCA+TGG | + | Chr2:6022113-6022132 | None:intergenic | 40.0% | |
| ATAGCATTAGAGACAGCAAG+GGG | - | Chr2:6022170-6022189 | MsG0280006773.01.T01:CDS | 40.0% | |
| GATAGCATTAGAGACAGCAA+GGG | - | Chr2:6022169-6022188 | MsG0280006773.01.T01:CDS | 40.0% | |
| TCTGTACTTGTGACTCTATG+TGG | - | Chr2:6022118-6022137 | MsG0280006773.01.T01:CDS | 40.0% | |
| TGATAGCATTAGAGACAGCA+AGG | - | Chr2:6022168-6022187 | MsG0280006773.01.T01:CDS | 40.0% | |
| ! | CGATATTTTGCAGCAACTTG+AGG | + | Chr2:6022262-6022281 | None:intergenic | 40.0% |
| ! | TATTTTGCAGCAACTTGAGG+TGG | + | Chr2:6022259-6022278 | None:intergenic | 40.0% |
| AATGCAAGAACAGTGCCTGT+AGG | + | Chr2:6022034-6022053 | None:intergenic | 45.0% | |
| AGCAACTTGAGGTGGAAGTT+TGG | + | Chr2:6022251-6022270 | None:intergenic | 45.0% | |
| CAAAAAACGCCAATTGCCAC+AGG | + | Chr2:6022421-6022440 | None:intergenic | 45.0% | |
| CCAATTCCATGTCTCTCAGT+AGG | + | Chr2:6022466-6022485 | None:intergenic | 45.0% | |
| CCTACTGAGAGACATGGAAT+TGG | - | Chr2:6022463-6022482 | MsG0280006773.01.T01:CDS | 45.0% | |
| GCATTTCCTACTGAGAGACA+TGG | - | Chr2:6022457-6022476 | MsG0280006773.01.T01:CDS | 45.0% | |
| TATGGGTTTGCCACCTTCAA+AGG | - | Chr2:6022202-6022221 | MsG0280006773.01.T01:CDS | 45.0% | |
| TGCCACCTTCAAAGGTTTGT+GGG | - | Chr2:6022210-6022229 | MsG0280006773.01.T01:CDS | 45.0% | |
| TTGCCACCTTCAAAGGTTTG+TGG | - | Chr2:6022209-6022228 | MsG0280006773.01.T01:CDS | 45.0% | |
| TTGGCAAACCTTCTACCTAC+AGG | - | Chr2:6022016-6022035 | MsG0280006773.01.T01:CDS | 45.0% | |
| ATTTCAGAGCACGGCGCATT+TGG | - | Chr2:6021997-6022016 | MsG0280006773.01.T01:CDS | 50.0% | |
| GAACAGTGCCTGTAGGTAGA+AGG | + | Chr2:6022027-6022046 | None:intergenic | 50.0% | |
| GCATGCGGTGATGTCAATTC+TGG | - | Chr2:6022303-6022322 | MsG0280006773.01.T01:CDS | 50.0% | |
| GGTTCCACTTCTGCGGAATT+CGG | - | Chr2:6021925-6021944 | MsG0280006773.01.T01:CDS | 50.0% | |
| ! | GACCCACAAACCTTTGAAGG+TGG | + | Chr2:6022215-6022234 | None:intergenic | 50.0% |
| ACCTCCGAATTCCGCAGAAG+TGG | + | Chr2:6021932-6021951 | None:intergenic | 55.0% | |
| TCCACTTCTGCGGAATTCGG+AGG | - | Chr2:6021928-6021947 | MsG0280006773.01.T01:CDS | 55.0% | |
| AATCCTCACGGCGTTGCCTG+TGG | - | Chr2:6022402-6022421 | MsG0280006773.01.T01:CDS | 60.0% | |
| ACGGCGTTGCCTGTGGCAAT+TGG | - | Chr2:6022409-6022428 | MsG0280006773.01.T01:CDS | 60.0% | |
| GCGGAATTCGGAGGTTCCAG+AGG | - | Chr2:6021937-6021956 | MsG0280006773.01.T01:CDS | 60.0% | |
| ACCAGGCTCCTCTCTGCCTC+TGG | + | Chr2:6021956-6021975 | None:intergenic | 65.0% | |
| CGGAGGTTCCAGAGGCAGAG+AGG | - | Chr2:6021945-6021964 | MsG0280006773.01.T01:CDS | 65.0% | |
| TCCAGAGGCAGAGAGGAGCC+TGG | - | Chr2:6021952-6021971 | MsG0280006773.01.T01:CDS | 65.0% | |
| TTGCCACAGGCAACGCCGTG+AGG | + | Chr2:6022408-6022427 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 6021872 | 6022507 | 6021872 | ID=MsG0280006773.01;Name=MsG0280006773.01 |
| Chr2 | mRNA | 6021872 | 6022507 | 6021872 | ID=MsG0280006773.01.T01;Parent=MsG0280006773.01;Name=MsG0280006773.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|211 |
| Chr2 | exon | 6021872 | 6022507 | 6021872 | ID=MsG0280006773.01.T01:exon:2488;Parent=MsG0280006773.01.T01 |
| Chr2 | CDS | 6021872 | 6022507 | 6021872 | ID=MsG0280006773.01.T01:cds;Parent=MsG0280006773.01.T01 |
| Gene Sequence |
| Protein sequence |
