AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006793.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006793.01.T01 MTR_2g015010 90.064 312 5 1 1 286 1 312 0.0 563
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006793.01.T01 AT2G36230 67.340 297 64 4 17 281 5 300 1.80e-134 383

Find 84 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTTTGCTGATGAGTTTC+TGG 0.046576 2:-6294488 MsG0280006793.01.T01:CDS
CATCATCTTTCAAATCTTTA+AGG 0.084762 2:+6296559 None:intergenic
TCAGACTCAAAATTAGTTAT+TGG 0.171929 2:+6296525 None:intergenic
TACAAACAAGATGGACTTAA+AGG 0.218596 2:-6296474 MsG0280006793.01.T01:CDS
GCTGATGAGTTTCTGGTTCA+TGG 0.240895 2:-6294481 MsG0280006793.01.T01:CDS
TTATTTGTAATAATTAAATA+TGG 0.301592 2:-6292893 MsG0280006793.01.T01:three_prime_UTR
AAAGATCTGGTTCGGATTGT+TGG 0.330297 2:-6294932 MsG0280006793.01.T01:CDS
AGATTATTGTGGCGGAGAAT+TGG 0.334881 2:+6296830 None:intergenic
ATCATCTTTCAAATCTTTAA+GGG 0.354407 2:+6296560 None:intergenic
GGAGGATTGAGGCGAAGAAA+AGG 0.385130 2:+6296806 None:intergenic
ATTTGCTTCACTGTTTCTCT+TGG 0.396285 2:-6293250 MsG0280006793.01.T01:three_prime_UTR
TTGTCAGTAAATATCTTTGT+TGG 0.417847 2:+6296962 None:intergenic
ATCAACATAGCATCGTGTTT+CGG 0.424655 2:-6293040 MsG0280006793.01.T01:three_prime_UTR
TGAACGGAGCATTGAATTGA+AGG 0.430403 2:+6296755 None:intergenic
AGTGAAGTCCAATCGTTCAT+TGG 0.431521 2:+6293005 None:intergenic
AAAGGCTAAAAGATCTGGTT+CGG 0.438014 2:-6294940 MsG0280006793.01.T01:CDS
GAGGCATTGCATGCTTATCC+TGG 0.440114 2:-6296399 MsG0280006793.01.T01:CDS
GGCAAGAAACTCCATTACTT+TGG 0.447787 2:+6294510 None:intergenic
ATGCAATCGTCACTGATAGA+TGG 0.449249 2:-6294561 MsG0280006793.01.T01:CDS
CAGAAACTCATCAGCAAAGT+TGG 0.449860 2:+6294489 None:intergenic
CTCATGACAAGTTAATGATC+AGG 0.453688 2:+6293176 None:intergenic
TTCGGATTGTTGGAAAAGAA+AGG 0.457181 2:-6294922 MsG0280006793.01.T01:CDS
TCTTGAAAGGCTAAAAGATC+TGG 0.465149 2:-6294945 MsG0280006793.01.T01:CDS
GTTAATGATCAGGCAAATGC+TGG 0.465854 2:+6293186 None:intergenic
TGTGACAGTAATGGACGATT+TGG 0.466828 2:-6293457 MsG0280006793.01.T01:CDS
TGTATTCAACAATGGACAAA+TGG 0.467573 2:-6294969 MsG0280006793.01.T01:CDS
AGATTTGTTGAAGTTAGGGG+TGG 0.471757 2:-6293290 MsG0280006793.01.T01:three_prime_UTR
TTTCGTGTTGGTGAAGAGGA+GGG 0.480898 2:+6296785 None:intergenic
GGAAAGTGAAACAAATTGTT+GGG 0.481539 2:-6296587 MsG0280006793.01.T01:CDS
GTGAAGTCCAATCGTTCATT+GGG 0.485184 2:+6293006 None:intergenic
TACCTTGTGAATATCGATGC+AGG 0.487164 2:+6296724 None:intergenic
TGTTACTGTTGGCAGTGCCT+TGG 0.487982 2:-6293400 MsG0280006793.01.T01:CDS
AAAGATTTGAAAGATGATGA+TGG 0.488673 2:-6296555 MsG0280006793.01.T01:CDS
CTTATAAAGATGTTGTAGCT+TGG 0.495973 2:-6293356 MsG0280006793.01.T01:CDS
GGGAAAGTGAAACAAATTGT+TGG 0.500496 2:-6296588 MsG0280006793.01.T01:CDS
TCTTGGTGTAACCTTTAGTC+CGG 0.506331 2:-6293233 MsG0280006793.01.T01:three_prime_UTR
CAGATTCCTGTGACATATGC+TGG 0.509158 2:-6293483 MsG0280006793.01.T01:CDS
GTGAAGAGGAGGGAGGATTG+AGG 0.512048 2:+6296795 None:intergenic
ACATGTTGTGATATAGTGTA+AGG 0.517762 2:-6293120 MsG0280006793.01.T01:three_prime_UTR
GAAGAGCTTGTAGCTCTGCT+TGG 0.519054 2:-6293596 MsG0280006793.01.T01:CDS
TCCATCTTGTTTGTAAAGAG+TGG 0.523508 2:+6296482 None:intergenic
TGCGAAGGCACCACCACGGA+TGG 0.532411 2:+6296859 None:intergenic
AAGATTTGAAAGATGATGAT+GGG 0.532729 2:-6296554 MsG0280006793.01.T01:CDS
GATTTGTTGAAGTTAGGGGT+GGG 0.536176 2:-6293289 MsG0280006793.01.T01:three_prime_UTR
ACCTTGTGAATATCGATGCA+GGG 0.538181 2:+6296725 None:intergenic
CGTGTTGGTGAAGAGGAGGG+AGG 0.540592 2:+6296788 None:intergenic
GTTCATGGCGTTGATGTTGA+AGG 0.540721 2:-6294466 MsG0280006793.01.T01:CDS
ATTAAGGAGATTCTTGTACA+TGG 0.545725 2:+6293077 None:intergenic
GATGCAGGGGCGGAATTGAA+CGG 0.547936 2:+6296739 None:intergenic
TGGAAAAGAAAGGCTTGTGT+TGG 0.548317 2:-6294912 MsG0280006793.01.T01:CDS
GAGTAAAGCTGCTTCTCTTG+AGG 0.548905 2:-6296418 MsG0280006793.01.T01:CDS
CGGATGGAAGGAGTGGAAGC+AGG 0.551050 2:+6296875 None:intergenic
GCCACTCTTTACAAACAAGA+TGG 0.552870 2:-6296483 MsG0280006793.01.T01:CDS
TTTAGATTTGTTGAAGTTAG+GGG 0.559058 2:-6293293 MsG0280006793.01.T01:three_prime_UTR
GGTGGTCATGTTATCATGCT+TGG 0.562038 2:-6296453 MsG0280006793.01.T01:CDS
AAGGCACCACCACGGATGGA+AGG 0.562520 2:+6296863 None:intergenic
ATGGACAAATGGATCTTGAA+AGG 0.564038 2:-6294958 MsG0280006793.01.T01:CDS
AGAGAAGCAGCTTTACTCAA+AGG 0.570052 2:+6296423 None:intergenic
TTGTATGCCCAATGAACGAT+TGG 0.572075 2:-6293013 MsG0280006793.01.T01:three_prime_UTR
TGGAATCAAACTAGAATACC+AGG 0.580246 2:+6296381 None:intergenic
GAGAATTGGAGAAGATGCGA+AGG 0.581362 2:+6296844 None:intergenic
GCTTCCACTCCTTCCATCCG+TGG 0.581913 2:-6296872 MsG0280006793.01.T01:CDS
AACAGCTGGAATGGATAATG+TGG 0.592450 2:-6293424 MsG0280006793.01.T01:CDS
TGCTGGTGGTGTGACAGTAA+TGG 0.600613 2:-6293466 MsG0280006793.01.T01:CDS
TCTGCTTGGAAAGCACTCAC+CGG 0.608734 2:-6293582 MsG0280006793.01.T01:intron
GTCACTTGATGCCAAAGTAA+TGG 0.608978 2:-6294521 MsG0280006793.01.T01:CDS
ACACCACCAGCATATGTCAC+AGG 0.617222 2:+6293477 None:intergenic
TTACAGTATGTATTCAACAA+TGG 0.619746 2:-6294977 MsG0280006793.01.T01:intron
TAGTGTAAGGCAGTGCATGT+AGG 0.621516 2:-6293107 MsG0280006793.01.T01:three_prime_UTR
TCCACTCCTTCCATCCGTGG+TGG 0.623461 2:-6296869 MsG0280006793.01.T01:CDS
GAAGAAAAGGAAGATTATTG+TGG 0.627520 2:+6296819 None:intergenic
GATAATGTGGATGTTACTGT+TGG 0.628001 2:-6293411 MsG0280006793.01.T01:CDS
CCCCTGCATCGATATTCACA+AGG 0.632190 2:-6296726 MsG0280006793.01.T01:intron
CCTTGTGAATATCGATGCAG+GGG 0.636281 2:+6296726 None:intergenic
GGGTGGGTGAGCTCTGAGCA+AGG 0.640540 2:-6293273 MsG0280006793.01.T01:three_prime_UTR
AACAAAGATATTTACTGACA+AGG 0.642593 2:-6296960 MsG0280006793.01.T01:five_prime_UTR
GAAAAGGAAGATTATTGTGG+CGG 0.644863 2:+6296822 None:intergenic
ACGGAGCATTGAATTGAAGG+AGG 0.650788 2:+6296758 None:intergenic
CAGTAATGGACGATTTGGAG+AGG 0.662099 2:-6293452 MsG0280006793.01.T01:CDS
ACCACCACGGATGGAAGGAG+TGG 0.663429 2:+6296868 None:intergenic
AAACAAGATGGACTTAAAGG+TGG 0.676603 2:-6296471 MsG0280006793.01.T01:CDS
TGTGAATATCGATGCAGGGG+CGG 0.677623 2:+6296729 None:intergenic
ATTCCTGTGACATATGCTGG+TGG 0.697373 2:-6293480 MsG0280006793.01.T01:CDS
AAGATGCGAAGGCACCACCA+CGG 0.721206 2:+6296855 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTATCAATATAACTT+GGG - Chr2:6294088-6294107 MsG0280006793.01.T01:intron 10.0%
!! AAATTTAACAAATGAAAGTA+GGG + Chr2:6295617-6295636 None:intergenic 15.0%
!! CATATATTAATATACTGTAA+TGG - Chr2:6294662-6294681 MsG0280006793.01.T01:intron 15.0%
!! CTATATTTATCAATATAACT+TGG - Chr2:6294087-6294106 MsG0280006793.01.T01:intron 15.0%
!! TAAATTTAACAAATGAAAGT+AGG + Chr2:6295618-6295637 None:intergenic 15.0%
!! TAATTGAATCAATAAATACA+TGG + Chr2:6294847-6294866 None:intergenic 15.0%
!!! ATATGATTTTAACGATATTA+AGG + Chr2:6296786-6296805 None:intergenic 15.0%
!!! ATTGTTTTCTTTATATTTGA+AGG - Chr2:6294981-6295000 MsG0280006793.01.T01:intron 15.0%
!!! GAAAAATTTGATTATTTTCT+TGG - Chr2:6293559-6293578 MsG0280006793.01.T01:intron 15.0%
!! AATTTAACAAATGAAAGTAG+GGG + Chr2:6295616-6295635 None:intergenic 20.0%
!! AGATGAAACATTAAAATAAC+AGG - Chr2:6295047-6295066 MsG0280006793.01.T01:intron 20.0%
!! ATCATCTTTCAAATCTTTAA+GGG + Chr2:6293287-6293306 None:intergenic 20.0%
!! CATTAGAACTTTATTGAAAA+AGG - Chr2:6293602-6293621 MsG0280006793.01.T01:CDS 20.0%
!! TATTAATATATGTTCGAAGA+AGG + Chr2:6294654-6294673 None:intergenic 20.0%
!! TGACTATATATAAATCTGTA+AGG - Chr2:6293823-6293842 MsG0280006793.01.T01:intron 20.0%
!! TTGACAATAAAATTAAGAGT+TGG + Chr2:6296295-6296314 None:intergenic 20.0%
!! TTTACAAAATGTGTATAGAT+AGG - Chr2:6294614-6294633 MsG0280006793.01.T01:intron 20.0%
!!! AAGTTTTATATTAAGTGCAT+TGG - Chr2:6295704-6295723 MsG0280006793.01.T01:intron 20.0%
!!! ACTTTTTGAGTATTTTTATC+AGG - Chr2:6295738-6295757 MsG0280006793.01.T01:intron 20.0%
!!! AGAAGTTAATATTGATTGAA+AGG + Chr2:6293148-6293167 None:intergenic 20.0%
!!! CTTTTTCAATAAAGTTCTAA+TGG + Chr2:6293604-6293623 None:intergenic 20.0%
!!! GAAATGTACATTAATTTGTT+TGG - Chr2:6294740-6294759 MsG0280006793.01.T01:intron 20.0%
!!! GAAGTTAATATTGATTGAAA+GGG + Chr2:6293147-6293166 None:intergenic 20.0%
!!! TTTATTTTGTACTTCAATCA+AGG - Chr2:6296027-6296046 MsG0280006793.01.T01:intron 20.0%
!!! TTTTAGATTTGTTGAAGTTA+GGG - Chr2:6296550-6296569 MsG0280006793.01.T01:CDS 20.0%
!!! TTTTTAGATTTGTTGAAGTT+AGG - Chr2:6296549-6296568 MsG0280006793.01.T01:CDS 20.0%
! AAAGATTTGAAAGATGATGA+TGG - Chr2:6293289-6293308 MsG0280006793.01.T01:three_prime_UTR 25.0%
! AACAAAGATATTTACTGACA+AGG - Chr2:6292884-6292903 MsG0280006793.01.T01:three_prime_UTR 25.0%
! AACTAACTCAATAACAAGAA+TGG + Chr2:6295456-6295475 None:intergenic 25.0%
! AAGATTTGAAAGATGATGAT+GGG - Chr2:6293290-6293309 MsG0280006793.01.T01:three_prime_UTR 25.0%
! AAGTTTATGATAGAACACTT+TGG - Chr2:6293650-6293669 MsG0280006793.01.T01:intron 25.0%
! AATATTTCAATGGATGCATA+TGG + Chr2:6296187-6296206 None:intergenic 25.0%
! CACTATCAGAAATATTTCAA+TGG + Chr2:6296197-6296216 None:intergenic 25.0%
! CATAAATTGCTAAAAATCAC+CGG + Chr2:6296633-6296652 None:intergenic 25.0%
! CATCATCTTTCAAATCTTTA+AGG + Chr2:6293288-6293307 None:intergenic 25.0%
! CTTAAGATAATTGCATACAA+GGG - Chr2:6295428-6295447 MsG0280006793.01.T01:intron 25.0%
! GAAACAGAAAAGTAAAAAAG+TGG + Chr2:6293489-6293508 None:intergenic 25.0%
! TAAAACTTCAAATGAATTGC+TGG + Chr2:6295692-6295711 None:intergenic 25.0%
! TCAATGCATTTGATCATAAT+TGG - Chr2:6295212-6295231 MsG0280006793.01.T01:intron 25.0%
! TCAGACTCAAAATTAGTTAT+TGG + Chr2:6293322-6293341 None:intergenic 25.0%
! TCTTAAGATAATTGCATACA+AGG - Chr2:6295427-6295446 MsG0280006793.01.T01:intron 25.0%
! TGAATCAATAAATACATGGA+CGG + Chr2:6294843-6294862 None:intergenic 25.0%
! TTACAGTATGTATTCAACAA+TGG - Chr2:6294867-6294886 MsG0280006793.01.T01:intron 25.0%
! TTGTCAGTAAATATCTTTGT+TGG + Chr2:6292885-6292904 None:intergenic 25.0%
!! ATCAAATGCATTGAAGTATT+GGG + Chr2:6295206-6295225 None:intergenic 25.0%
!! ATTGTCAAATGTCAACTTTT+AGG - Chr2:6296307-6296326 MsG0280006793.01.T01:intron 25.0%
!! TAATTGTTTGAGTTACATTC+AGG - Chr2:6294258-6294277 MsG0280006793.01.T01:intron 25.0%
!! TTTTTACATGTGGGTTTTTA+GGG - Chr2:6293194-6293213 MsG0280006793.01.T01:three_prime_UTR 25.0%
!!! AACATCATGAAATTGTTTTG+AGG + Chr2:6295177-6295196 None:intergenic 25.0%
!!! AAGAACATATGTTTTTCAAG+TGG - Chr2:6294714-6294733 MsG0280006793.01.T01:intron 25.0%
!!! AGAAGAAGTGTTTTTGTATT+TGG - Chr2:6295949-6295968 MsG0280006793.01.T01:intron 25.0%
!!! ATTCTTGTTATTGAGTTAGT+TGG - Chr2:6295455-6295474 MsG0280006793.01.T01:intron 25.0%
!!! CATTTCTTTTTAAGCACTTA+TGG - Chr2:6295236-6295255 MsG0280006793.01.T01:intron 25.0%
!!! TTCTTGTTATTGAGTTAGTT+GGG - Chr2:6295456-6295475 MsG0280006793.01.T01:intron 25.0%
!!! TTGTTCTGTTTTTTGTTTCT+TGG - Chr2:6295556-6295575 MsG0280006793.01.T01:intron 25.0%
!!! TTTAGATTTGTTGAAGTTAG+GGG - Chr2:6296551-6296570 MsG0280006793.01.T01:CDS 25.0%
AAGAGCAATGCCATAAAATA+TGG - Chr2:6295088-6295107 MsG0280006793.01.T01:intron 30.0%
AATATATGTTCGAAGAAGGT+TGG + Chr2:6294650-6294669 None:intergenic 30.0%
AATGCCATAAAATATGGTGT+GGG - Chr2:6295094-6295113 MsG0280006793.01.T01:intron 30.0%
ACAGAAAAGTAAAAAAGTGG+TGG + Chr2:6293486-6293505 None:intergenic 30.0%
ACATGTTGTGATATAGTGTA+AGG - Chr2:6296724-6296743 MsG0280006793.01.T01:intron 30.0%
AGGATGTGATGTTATAAAGA+GGG - Chr2:6294426-6294445 MsG0280006793.01.T01:intron 30.0%
ATAACTTAACATACTTCCAC+AGG + Chr2:6296054-6296073 None:intergenic 30.0%
ATCCACAATAAGTTTAGTAG+AGG + Chr2:6294188-6294207 None:intergenic 30.0%
ATTAAGGAGATTCTTGTACA+TGG + Chr2:6296770-6296789 None:intergenic 30.0%
CAAAGTCCAATATCAACAAA+AGG + Chr2:6294026-6294045 None:intergenic 30.0%
CAATTCATTACAAAAAGGAG+CGG - Chr2:6293171-6293190 MsG0280006793.01.T01:three_prime_UTR 30.0%
CTAAACTTATTGTGGATGAA+TGG - Chr2:6294191-6294210 MsG0280006793.01.T01:intron 30.0%
CTTATAAAGATGTTGTAGCT+TGG - Chr2:6296488-6296507 MsG0280006793.01.T01:CDS 30.0%
CTTGCCAATTCATTACAAAA+AGG - Chr2:6293166-6293185 MsG0280006793.01.T01:three_prime_UTR 30.0%
GAAGAAAAGGAAGATTATTG+TGG + Chr2:6293028-6293047 None:intergenic 30.0%
GATCAAATGCATTGAAGTAT+TGG + Chr2:6295207-6295226 None:intergenic 30.0%
GATGAATGGTAAAATTAAGG+TGG - Chr2:6294205-6294224 MsG0280006793.01.T01:intron 30.0%
GGAAAGTGAAACAAATTGTT+GGG - Chr2:6293257-6293276 MsG0280006793.01.T01:three_prime_UTR 30.0%
GTGAATTGCATTCAAGAAAA+AGG - Chr2:6295003-6295022 MsG0280006793.01.T01:intron 30.0%
GTGGATGAATGGTAAAATTA+AGG - Chr2:6294202-6294221 MsG0280006793.01.T01:intron 30.0%
TAGAATTAGTATGTGATGCT+AGG - Chr2:6293531-6293550 MsG0280006793.01.T01:intron 30.0%
TAGGATGTGATGTTATAAAG+AGG - Chr2:6294425-6294444 MsG0280006793.01.T01:intron 30.0%
TGCACTTTCACTTAATCATA+TGG - Chr2:6293742-6293761 MsG0280006793.01.T01:intron 30.0%
TGTATTCAACAATGGACAAA+TGG - Chr2:6294875-6294894 MsG0280006793.01.T01:intron 30.0%
TTCCTCTACTAAACTTATTG+TGG - Chr2:6294183-6294202 MsG0280006793.01.T01:intron 30.0%
TTGAACTCCAAATCTTGAAA+AGG - Chr2:6293901-6293920 MsG0280006793.01.T01:intron 30.0%
! GTTTTTACATGTGGGTTTTT+AGG - Chr2:6293193-6293212 MsG0280006793.01.T01:three_prime_UTR 30.0%
! TACAAACAAGATGGACTTAA+AGG - Chr2:6293370-6293389 MsG0280006793.01.T01:CDS 30.0%
! TAGCACATATTTTTCACAGT+AGG + Chr2:6294795-6294814 None:intergenic 30.0%
! TGTTATTGAGAGCTTTTATC+AGG - Chr2:6293981-6294000 MsG0280006793.01.T01:intron 30.0%
! TTTGCTGTGTTATTAGCTAA+TGG - Chr2:6295889-6295908 MsG0280006793.01.T01:intron 30.0%
!! CACTATTTCTAATGTTTCAG+AGG - Chr2:6295590-6295609 MsG0280006793.01.T01:intron 30.0%
!! GCTTTTATCAGGTGTTTTTA+TGG - Chr2:6293992-6294011 MsG0280006793.01.T01:intron 30.0%
!! GGCATTTTCTTTGATTTTCA+TGG - Chr2:6294131-6294150 MsG0280006793.01.T01:intron 30.0%
!! TTTTACATGTGGGTTTTTAG+GGG - Chr2:6293195-6293214 MsG0280006793.01.T01:three_prime_UTR 30.0%
!! TTTTGAGTCTGATAAATCAG+CGG - Chr2:6293330-6293349 MsG0280006793.01.T01:CDS 30.0%
!!! CTGTTTTTTGTTTCTTGGTT+TGG - Chr2:6295561-6295580 MsG0280006793.01.T01:intron 30.0%
AAAGGCTAAAAGATCTGGTT+CGG - Chr2:6294904-6294923 MsG0280006793.01.T01:CDS 35.0%
AATTAGTATGTGATGCTAGG+TGG - Chr2:6293534-6293553 MsG0280006793.01.T01:intron 35.0%
AGAAGAGAATTAGACGAATC+TGG - Chr2:6294451-6294470 MsG0280006793.01.T01:intron 35.0%
ATACCCAAAAGAAAGAGAGA+AGG + Chr2:6294325-6294344 None:intergenic 35.0%
ATCAACATAGCATCGTGTTT+CGG - Chr2:6296804-6296823 MsG0280006793.01.T01:CDS 35.0%
ATGCCATAAAATATGGTGTG+GGG - Chr2:6295095-6295114 MsG0280006793.01.T01:intron 35.0%
ATGGACAAATGGATCTTGAA+AGG - Chr2:6294886-6294905 MsG0280006793.01.T01:intron 35.0%
CAATGCCATAAAATATGGTG+TGG - Chr2:6295093-6295112 MsG0280006793.01.T01:intron 35.0%
CTCATGACAAGTTAATGATC+AGG + Chr2:6296671-6296690 None:intergenic 35.0%
GAAAAGGAAGATTATTGTGG+CGG + Chr2:6293025-6293044 None:intergenic 35.0%
GAAAATGCCAAATAAGTCGA+AGG + Chr2:6294120-6294139 None:intergenic 35.0%
GACCTAGAGAGAAAAATACA+GGG + Chr2:6294542-6294561 None:intergenic 35.0%
GATAATGTGGATGTTACTGT+TGG - Chr2:6296433-6296452 MsG0280006793.01.T01:CDS 35.0%
GCTAGCTAACTAACTATGAT+TGG + Chr2:6294386-6294405 None:intergenic 35.0%
GGGAAAGTGAAACAAATTGT+TGG - Chr2:6293256-6293275 MsG0280006793.01.T01:three_prime_UTR 35.0%
TACCCAAAAGAAAGAGAGAA+GGG + Chr2:6294324-6294343 None:intergenic 35.0%
TATAGTCATGAAGTCAGTCA+AGG + Chr2:6294072-6294091 None:intergenic 35.0%
TCACTCTACAGAGACAAAAA+AGG + Chr2:6293861-6293880 None:intergenic 35.0%
TCTTGAAAGGCTAAAAGATC+TGG - Chr2:6294899-6294918 MsG0280006793.01.T01:CDS 35.0%
TGACCTAGAGAGAAAAATAC+AGG + Chr2:6294543-6294562 None:intergenic 35.0%
TGATATTGGACTTTGTACAG+CGG - Chr2:6294031-6294050 MsG0280006793.01.T01:intron 35.0%
TGGAATCAAACTAGAATACC+AGG + Chr2:6293466-6293485 None:intergenic 35.0%
TGGAGTTCAAATTTGATGCA+TGG + Chr2:6293891-6293910 None:intergenic 35.0%
TTACAGATGTCACAATGACA+TGG + Chr2:6294294-6294313 None:intergenic 35.0%
TTCGGATTGTTGGAAAAGAA+AGG - Chr2:6294922-6294941 MsG0280006793.01.T01:CDS 35.0%
! AAACAAGATGGACTTAAAGG+TGG - Chr2:6293373-6293392 MsG0280006793.01.T01:CDS 35.0%
! ATCCCTTCTCTCTTTCTTTT+GGG - Chr2:6294319-6294338 MsG0280006793.01.T01:intron 35.0%
! ATTTGCTTCACTGTTTCTCT+TGG - Chr2:6296594-6296613 MsG0280006793.01.T01:intron 35.0%
! CGCTCCTTTTTGTAATGAAT+TGG + Chr2:6293173-6293192 None:intergenic 35.0%
! CTTGATTGTTGTTAACTCAG+AGG - Chr2:6293716-6293735 MsG0280006793.01.T01:intron 35.0%
! GATTGGCTAGTTAACTAACT+AGG + Chr2:6294369-6294388 None:intergenic 35.0%
! GGGTATTCAACTTTTACGAT+AGG - Chr2:6294339-6294358 MsG0280006793.01.T01:intron 35.0%
! TACCCTGTATTTTTCTCTCT+AGG - Chr2:6294537-6294556 MsG0280006793.01.T01:CDS 35.0%
! TAGCGACCTTTTGTTGATAT+TGG - Chr2:6294017-6294036 MsG0280006793.01.T01:intron 35.0%
! TATCCCTTCTCTCTTTCTTT+TGG - Chr2:6294318-6294337 MsG0280006793.01.T01:intron 35.0%
! TCCATCTTGTTTGTAAAGAG+TGG + Chr2:6293365-6293384 None:intergenic 35.0%
! TGTTTGAGTTACATTCAGGA+AGG - Chr2:6294262-6294281 MsG0280006793.01.T01:intron 35.0%
!! GCTTCTTCCTTTTCAAGATT+TGG + Chr2:6293911-6293930 None:intergenic 35.0%
!! TGGGTCATTGTGTTTTTGTA+GGG - Chr2:6293235-6293254 MsG0280006793.01.T01:three_prime_UTR 35.0%
AAATACAGGGTACAATCCCT+AGG + Chr2:6294529-6294548 None:intergenic 40.0%
AAATCACTGAATCAGCCCTA+GGG - Chr2:6294510-6294529 MsG0280006793.01.T01:CDS 40.0%
AACAGCTGGAATGGATAATG+TGG - Chr2:6296420-6296439 MsG0280006793.01.T01:CDS 40.0%
AAGAATGCACCTAGCAATCA+CGG - Chr2:6296106-6296125 MsG0280006793.01.T01:intron 40.0%
AATACAGGGTACAATCCCTA+GGG + Chr2:6294528-6294547 None:intergenic 40.0%
AATGATCACTCCTTGTAGGT+TGG - Chr2:6296217-6296236 MsG0280006793.01.T01:intron 40.0%
AATTAAGGTGGTCTGCAAGT+TGG - Chr2:6294217-6294236 MsG0280006793.01.T01:intron 40.0%
ACCTTGTGAATATCGATGCA+GGG + Chr2:6293122-6293141 None:intergenic 40.0%
AGAGAAGCAGCTTTACTCAA+AGG + Chr2:6293424-6293443 None:intergenic 40.0%
AGTGAAGTCCAATCGTTCAT+TGG + Chr2:6296842-6296861 None:intergenic 40.0%
AGTGAATGATCACTCCTTGT+AGG - Chr2:6296213-6296232 MsG0280006793.01.T01:intron 40.0%
ATATTTACCTTCCTGAGCAC+TGG + Chr2:6295265-6295284 None:intergenic 40.0%
ATGATCACTCCTTGTAGGTT+GGG - Chr2:6296218-6296237 MsG0280006793.01.T01:intron 40.0%
ATGCAATCGTCACTGATAGA+TGG - Chr2:6295283-6295302 MsG0280006793.01.T01:intron 40.0%
ATTAAGAGTTGGCTACCAAC+CGG + Chr2:6296284-6296303 None:intergenic 40.0%
CAACTTTGCTGATGAGTTTC+TGG - Chr2:6295356-6295375 MsG0280006793.01.T01:intron 40.0%
CAGAAACTCATCAGCAAAGT+TGG + Chr2:6295358-6295377 None:intergenic 40.0%
CAGAGACAAAAAAGGTGAGA+TGG + Chr2:6293853-6293872 None:intergenic 40.0%
CTAACCCCTTTAACCAACTA+AGG + Chr2:6294408-6294427 None:intergenic 40.0%
CTTAGTTGGTTAAAGGGGTT+AGG - Chr2:6294406-6294425 MsG0280006793.01.T01:intron 40.0%
GAGAATGCCTTCGACTTATT+TGG - Chr2:6294110-6294129 MsG0280006793.01.T01:intron 40.0%
GAGGATAAAAACAGCTGGAA+TGG - Chr2:6296411-6296430 MsG0280006793.01.T01:CDS 40.0%
GCCACTCTTTACAAACAAGA+TGG - Chr2:6293361-6293380 MsG0280006793.01.T01:CDS 40.0%
GCGTAGTTACAGATCAAGAT+TGG + Chr2:6294770-6294789 None:intergenic 40.0%
GCTAAAAATCACCGGACTAA+AGG + Chr2:6296625-6296644 None:intergenic 40.0%
GGATCTCAGTTGCAGAAAAA+AGG - Chr2:6294953-6294972 MsG0280006793.01.T01:CDS 40.0%
GGCAAGAAACTCCATTACTT+TGG + Chr2:6295337-6295356 None:intergenic 40.0%
GTCACTTGATGCCAAAGTAA+TGG - Chr2:6295323-6295342 MsG0280006793.01.T01:intron 40.0%
GTGAAGTCCAATCGTTCATT+GGG + Chr2:6296841-6296860 None:intergenic 40.0%
GTTAATGATCAGGCAAATGC+TGG + Chr2:6296661-6296680 None:intergenic 40.0%
TAACCAGCAAAAAGTCTCTG+TGG - Chr2:6296513-6296532 MsG0280006793.01.T01:CDS 40.0%
TACCTTGTGAATATCGATGC+AGG + Chr2:6293123-6293142 None:intergenic 40.0%
TCATCAATTCCCAACCTACA+AGG + Chr2:6296230-6296249 None:intergenic 40.0%
TCTGGAAATAAGTCAGAGCT+AGG - Chr2:6294469-6294488 MsG0280006793.01.T01:CDS 40.0%
TGCCATAAAATATGGTGTGG+GGG - Chr2:6295096-6295115 MsG0280006793.01.T01:intron 40.0%
TGGAAAAGAAAGGCTTGTGT+TGG - Chr2:6294932-6294951 MsG0280006793.01.T01:CDS 40.0%
TTGGAGAGGATAAAAACAGC+TGG - Chr2:6296406-6296425 MsG0280006793.01.T01:CDS 40.0%
TTGTATGCCCAATGAACGAT+TGG - Chr2:6296831-6296850 MsG0280006793.01.T01:CDS 40.0%
! AAAGGAGCGGTTTTTACATG+TGG - Chr2:6293184-6293203 MsG0280006793.01.T01:three_prime_UTR 40.0%
! AAGGAGCGGTTTTTACATGT+GGG - Chr2:6293185-6293204 MsG0280006793.01.T01:three_prime_UTR 40.0%
! AGATTATTGTGGCGGAGAAT+TGG + Chr2:6293017-6293036 None:intergenic 40.0%
! TCTTGGTGTAACCTTTAGTC+CGG - Chr2:6296611-6296630 MsG0280006793.01.T01:intron 40.0%
!! AAAGATCTGGTTCGGATTGT+TGG - Chr2:6294912-6294931 MsG0280006793.01.T01:CDS 40.0%
!! AGATTTGTTGAAGTTAGGGG+TGG - Chr2:6296554-6296573 MsG0280006793.01.T01:CDS 40.0%
!! AGATTTTCGTGTTGGTGAAG+AGG + Chr2:6293066-6293085 None:intergenic 40.0%
!! AGCTAGCCTTAGTTGGTTAA+AGG - Chr2:6294399-6294418 MsG0280006793.01.T01:intron 40.0%
!! AGTCAGTCAAGGCATTTTCA+AGG + Chr2:6294061-6294080 None:intergenic 40.0%
!! CTAGCCTTAGTTGGTTAAAG+GGG - Chr2:6294401-6294420 MsG0280006793.01.T01:intron 40.0%
!! GATTTGTTGAAGTTAGGGGT+GGG - Chr2:6296555-6296574 MsG0280006793.01.T01:CDS 40.0%
!! GCTAGCCTTAGTTGGTTAAA+GGG - Chr2:6294400-6294419 MsG0280006793.01.T01:intron 40.0%
!! GGGTCATTGTGTTTTTGTAG+GGG - Chr2:6293236-6293255 MsG0280006793.01.T01:three_prime_UTR 40.0%
!! GTGGGTCATTGTGTTTTTGT+AGG - Chr2:6293234-6293253 MsG0280006793.01.T01:three_prime_UTR 40.0%
!! GTTAGTTAGCTAGCCTTAGT+TGG - Chr2:6294392-6294411 MsG0280006793.01.T01:intron 40.0%
!! TCAAGCCTTATTCCACTAAG+CGG + Chr2:6293701-6293720 None:intergenic 40.0%
!! TGAACGGAGCATTGAATTGA+AGG + Chr2:6293092-6293111 None:intergenic 40.0%
!! TGTGACAGTAATGGACGATT+TGG - Chr2:6296387-6296406 MsG0280006793.01.T01:intron 40.0%
!!! GGAATGGCATTTTAAGACGT+GGG - Chr2:6293216-6293235 MsG0280006793.01.T01:three_prime_UTR 40.0%
ACTTATGGTTTCCAGTGCTC+AGG - Chr2:6295251-6295270 MsG0280006793.01.T01:intron 45.0%
AGAATGCACCTAGCAATCAC+GGG - Chr2:6296107-6296126 MsG0280006793.01.T01:intron 45.0%
ATTCCCCCCAAAAATGTCCA+AGG + Chr2:6296464-6296483 None:intergenic 45.0%
ATTCCTGTGACATATGCTGG+TGG - Chr2:6296364-6296383 MsG0280006793.01.T01:intron 45.0%
CAAATCACTGAATCAGCCCT+AGG - Chr2:6294509-6294528 MsG0280006793.01.T01:CDS 45.0%
CAGATTCCTGTGACATATGC+TGG - Chr2:6296361-6296380 MsG0280006793.01.T01:intron 45.0%
CCATAAAATATGGTGTGGGG+GGG - Chr2:6295098-6295117 MsG0280006793.01.T01:intron 45.0%
CCTTGTGAATATCGATGCAG+GGG + Chr2:6293121-6293140 None:intergenic 45.0%
CGAACTCGTGAAACTACTTC+TGG + Chr2:6295137-6295156 None:intergenic 45.0%
GAGAATTGGAGAAGATGCGA+AGG + Chr2:6293003-6293022 None:intergenic 45.0%
GAGTAAAGCTGCTTCTCTTG+AGG - Chr2:6293426-6293445 MsG0280006793.01.T01:CDS 45.0%
GCCATAAAATATGGTGTGGG+GGG - Chr2:6295097-6295116 MsG0280006793.01.T01:intron 45.0%
GGTGGTCATGTTATCATGCT+TGG - Chr2:6293391-6293410 MsG0280006793.01.T01:CDS 45.0%
GTACTTCAATCAAGGTCCTG+TGG - Chr2:6296035-6296054 MsG0280006793.01.T01:intron 45.0%
TAGTGTAAGGCAGTGCATGT+AGG - Chr2:6296737-6296756 MsG0280006793.01.T01:CDS 45.0%
TCAGAGCTAGGAGAGTTGAA+GGG - Chr2:6294481-6294500 MsG0280006793.01.T01:CDS 45.0%
! AAGCCACAGAGACTTTTTGC+TGG + Chr2:6296519-6296538 None:intergenic 45.0%
! CAGTGCCTTGGACATTTTTG+GGG - Chr2:6296456-6296475 MsG0280006793.01.T01:CDS 45.0%
! GAAGGAGGAGATTTTCGTGT+TGG + Chr2:6293074-6293093 None:intergenic 45.0%
! GCAGTGCCTTGGACATTTTT+GGG - Chr2:6296455-6296474 MsG0280006793.01.T01:CDS 45.0%
! TTTCGTGTTGGTGAAGAGGA+GGG + Chr2:6293062-6293081 None:intergenic 45.0%
!! AAGCCTTATTCCACTAAGCG+GGG + Chr2:6293699-6293718 None:intergenic 45.0%
!! ACGGAGCATTGAATTGAAGG+AGG + Chr2:6293089-6293108 None:intergenic 45.0%
!! AGTGCCTTGGACATTTTTGG+GGG - Chr2:6296457-6296476 MsG0280006793.01.T01:CDS 45.0%
!! CAAGCCTTATTCCACTAAGC+GGG + Chr2:6293700-6293719 None:intergenic 45.0%
!! CAGTAATGGACGATTTGGAG+AGG - Chr2:6296392-6296411 MsG0280006793.01.T01:CDS 45.0%
!! CATGTGGGTTTTTAGGGGAA+TGG - Chr2:6293200-6293219 MsG0280006793.01.T01:three_prime_UTR 45.0%
!! GCTGATGAGTTTCTGGTTCA+TGG - Chr2:6295363-6295382 MsG0280006793.01.T01:intron 45.0%
!! GGACATTTTTGGGGGGAATT+TGG - Chr2:6296465-6296484 MsG0280006793.01.T01:CDS 45.0%
!! GTTCATGGCGTTGATGTTGA+AGG - Chr2:6295378-6295397 MsG0280006793.01.T01:intron 45.0%
!! TTTTCGTGTTGGTGAAGAGG+AGG + Chr2:6293063-6293082 None:intergenic 45.0%
!!! GGGAATGGCATTTTAAGACG+TGG - Chr2:6293215-6293234 MsG0280006793.01.T01:three_prime_UTR 45.0%
!! TTATTTGTAATAATTAAATA+TGG - Chr2:6296951-6296970 MsG0280006793.01.T01:five_prime_UTR 5.0%
ACACCACCAGCATATGTCAC+AGG + Chr2:6296370-6296389 None:intergenic 50.0%
ATGGTTTCCAGTGCTCAGGA+AGG - Chr2:6295255-6295274 MsG0280006793.01.T01:intron 50.0%
GGAGGATTGAGGCGAAGAAA+AGG + Chr2:6293041-6293060 None:intergenic 50.0%
GTCAGAGCTAGGAGAGTTGA+AGG - Chr2:6294480-6294499 MsG0280006793.01.T01:CDS 50.0%
TGTGAATATCGATGCAGGGG+CGG + Chr2:6293118-6293137 None:intergenic 50.0%
TGTTACTGTTGGCAGTGCCT+TGG - Chr2:6296444-6296463 MsG0280006793.01.T01:CDS 50.0%
! CCCCCCCACACCATATTTTA+TGG + Chr2:6295101-6295120 None:intergenic 50.0%
! CCCCTGCATCGATATTCACA+AGG - Chr2:6293118-6293137 MsG0280006793.01.T01:three_prime_UTR 50.0%
! GAAGAGCTTGTAGCTCTGCT+TGG - Chr2:6296248-6296267 MsG0280006793.01.T01:intron 50.0%
! GGCAGTGCCTTGGACATTTT+TGG - Chr2:6296454-6296473 MsG0280006793.01.T01:CDS 50.0%
! TAAGGTGGTCTGCAAGTTGG+AGG - Chr2:6294220-6294239 MsG0280006793.01.T01:intron 50.0%
! TGCTGGTGGTGTGACAGTAA+TGG - Chr2:6296378-6296397 MsG0280006793.01.T01:intron 50.0%
!! GAGGCATTGCATGCTTATCC+TGG - Chr2:6293445-6293464 MsG0280006793.01.T01:CDS 50.0%
!! GTGCCTTGGACATTTTTGGG+GGG - Chr2:6296458-6296477 MsG0280006793.01.T01:CDS 50.0%
!! TCTGCTTGGAAAGCACTCAC+CGG - Chr2:6296262-6296281 MsG0280006793.01.T01:intron 50.0%
ATGAAGTCGACCCCGCTTAG+TGG - Chr2:6293686-6293705 MsG0280006793.01.T01:intron 55.0%
CGACCCCGCTTAGTGGAATA+AGG - Chr2:6293693-6293712 MsG0280006793.01.T01:intron 55.0%
GAACCAAGCCCGTGATTGCT+AGG + Chr2:6296118-6296137 None:intergenic 55.0%
GATGCAGGGGCGGAATTGAA+CGG + Chr2:6293108-6293127 None:intergenic 55.0%
GTGAAGAGGAGGGAGGATTG+AGG + Chr2:6293052-6293071 None:intergenic 55.0%
!! AAGATGCGAAGGCACCACCA+CGG + Chr2:6292992-6293011 None:intergenic 55.0%
!! CTTGGAAAGCACTCACCGGT+TGG - Chr2:6296266-6296285 MsG0280006793.01.T01:intron 55.0%
ACCACCACGGATGGAAGGAG+TGG + Chr2:6292979-6292998 None:intergenic 60.0%
CGGATGGAAGGAGTGGAAGC+AGG + Chr2:6292972-6292991 None:intergenic 60.0%
GCACCTAGCAATCACGGGCT+TGG - Chr2:6296112-6296131 MsG0280006793.01.T01:intron 60.0%
GCTTCCACTCCTTCCATCCG+TGG - Chr2:6292972-6292991 MsG0280006793.01.T01:three_prime_UTR 60.0%
TCCACTCCTTCCATCCGTGG+TGG - Chr2:6292975-6292994 MsG0280006793.01.T01:three_prime_UTR 60.0%
! AAGGCACCACCACGGATGGA+AGG + Chr2:6292984-6293003 None:intergenic 60.0%
! CGTGTTGGTGAAGAGGAGGG+AGG + Chr2:6293059-6293078 None:intergenic 60.0%
! GGTGGTCTGCAAGTTGGAGG+TGG - Chr2:6294223-6294242 MsG0280006793.01.T01:intron 60.0%
!! GGGTGGGTGAGCTCTGAGCA+AGG - Chr2:6296571-6296590 MsG0280006793.01.T01:CDS 65.0%
!! TGCGAAGGCACCACCACGGA+TGG + Chr2:6292988-6293007 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 6292879 6296987 6292879 ID=MsG0280006793.01;Name=MsG0280006793.01
Chr2 mRNA 6292879 6296987 6292879 ID=MsG0280006793.01.T01;Parent=MsG0280006793.01;Name=MsG0280006793.01.T01;_AED=0.33;_eAED=0.34;_QI=81|0.8|0.83|1|0.8|0.83|6|447|286
Chr2 exon 6292879 6293502 6292879 ID=MsG0280006793.01.T01:exon:2634;Parent=MsG0280006793.01.T01
Chr2 exon 6293583 6293631 6293583 ID=MsG0280006793.01.T01:exon:2633;Parent=MsG0280006793.01.T01
Chr2 exon 6294460 6294593 6294460 ID=MsG0280006793.01.T01:exon:2632;Parent=MsG0280006793.01.T01
Chr2 exon 6294892 6294993 6294892 ID=MsG0280006793.01.T01:exon:2631;Parent=MsG0280006793.01.T01
Chr2 exon 6296392 6296610 6296392 ID=MsG0280006793.01.T01:exon:2630;Parent=MsG0280006793.01.T01
Chr2 exon 6296727 6296987 6296727 ID=MsG0280006793.01.T01:exon:2629;Parent=MsG0280006793.01.T01
Chr2 five_prime_UTR 6296907 6296987 6296907 ID=MsG0280006793.01.T01:five_prime_utr;Parent=MsG0280006793.01.T01
Chr2 CDS 6296727 6296906 6296727 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 CDS 6296392 6296610 6296392 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 CDS 6294892 6294993 6294892 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 CDS 6294460 6294593 6294460 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 CDS 6293583 6293631 6293583 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 CDS 6293326 6293502 6293326 ID=MsG0280006793.01.T01:cds;Parent=MsG0280006793.01.T01
Chr2 three_prime_UTR 6292879 6293325 6292879 ID=MsG0280006793.01.T01:three_prime_utr;Parent=MsG0280006793.01.T01
Gene Sequence

>MsG0280006793.01.T01

ATGCGCAGTCCTGCTTCCACTCCTTCCATCCGTGGTGGTGCCTTCGCATCTTCTCCAATTCTCCGCCACAATAATCTTCCTTTTCTTCGCCTCAATCCTCCCTCCTCTTCACCAACACGAAAATCTCCTCCTTCAATTCAATGCTCCGTTCAATTCCGCCCCTGCATCGATATTCACAAGGGGAAAGTGAAACAAATTGTTGGGTCAACCCTTAAAGATTTGAAAGATGATGATGGGTCAGATCCAATAACTAATTTTGAGTCTGATAAATCAGCGGCTGAGTATGCCACTCTTTACAAACAAGATGGACTTAAAGGTGGTCATGTTATCATGCTTGGTGCTGATCCTTTGAGTAAAGCTGCTTCTCTTGAGGCATTGCATGCTTATCCTGGTATTCTATATGTATTCAACAATGGACAAATGGATCTTGAAAGGCTAAAAGATCTGGTTCGGATTGTTGGAAAAGAAAGGCTTGTGTTGGATCTCAGTTGCAGAAAAAAGGAAGGTAAATATGCAATCGTCACTGATAGATGGCAGAAGTTCAGTGATGTGTCACTTGATGCCAAAGTAATGGAGTTTCTTGCCAACTTTGCTGATGAGTTTCTGGTTCATGGCGTTGATGTTGAAGGAAAGAAGTTGGGAATTGATGAAGAGCTTGTAGCTCTGCTTGGAAAGCACTCACCGATTCCTGTGACATATGCTGGTGGTGTGACAGTAATGGACGATTTGGAGAGGATAAAAACAGCTGGAATGGATAATGTGGATGTTACTGTTGGCAGTGCCTTGGACATTTTTGGGGGGAATTTGGCTTATAAAGATGTTGTAGCTTGGCATAACCAGCAAAAAGTCTCTGTGGCTTAA
Protein sequence

>MsG0280006793.01.T01

MRSPASTPSIRGGAFASSPILRHNNLPFLRLNPPSSSPTRKSPPSIQCSVQFRPCIDIHKGKVKQIVGSTLKDLKDDDGSDPITNFESDKSAAEYATLYKQDGLKGGHVIMLGADPLSKAASLEALHAYPGILYVFNNGQMDLERLKDLVRIVGKERLVLDLSCRKKEGKYAIVTDRWQKFSDVSLDAKVMEFLANFADEFLVHGVDVEGKKLGIDEELVALLGKHSPIPVTYAGGVTVMDDLERIKTAGMDNVDVTVGSALDIFGGNLAYKDVVAWHNQQKVSVA*