Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006874.01 | MsG0580026122.01 | 0.806080 | 9.785036e-50 | 5.262775e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGGACAAATGTGTTTA+TGG | 0.232453 | 2:+7466802 | MsG0280006874.01.T01:CDS |
| CTTGAAAGATTTATCTGGTT+TGG | 0.291375 | 2:-7467020 | None:intergenic |
| ATCGACTTGAAAGATTTATC+TGG | 0.321621 | 2:-7467025 | None:intergenic |
| TTTAATAGCACAGATGGTGC+TGG | 0.508221 | 2:+7466993 | MsG0280006874.01.T01:intron |
| CAGTGATGAAGACGGAAGAG+AGG | 0.574395 | 2:+7467049 | MsG0280006874.01.T01:CDS |
| AAATGCGAGAAAGTCAAGAG+AGG | 0.636328 | 2:+7466786 | None:intergenic |
| AGTCGATGCAGTGATGAAGA+CGG | 0.639721 | 2:+7467041 | MsG0280006874.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATAAATTGTTTTTATCT+TGG | + | Chr2:7467075-7467094 | MsG0280006874.01.T01:CDS | 10.0% |
| !!! | TAATATTAAATCTGAACTTT+TGG | - | Chr2:7466931-7466950 | None:intergenic | 15.0% |
| !!! | TTTTATTTTAATAGCACAGA+TGG | + | Chr2:7466987-7467006 | MsG0280006874.01.T01:intron | 20.0% |
| !!! | TTTTTATCTTGGATATGTTT+AGG | + | Chr2:7467086-7467105 | MsG0280006874.01.T01:CDS | 20.0% |
| CTTGAAAGATTTATCTGGTT+TGG | - | Chr2:7467023-7467042 | None:intergenic | 30.0% | |
| ! | TATTTTACAAGCTGCAAAAC+AGG | + | Chr2:7466877-7466896 | MsG0280006874.01.T01:CDS | 30.0% |
| !! | ATCGACTTGAAAGATTTATC+TGG | - | Chr2:7467028-7467047 | None:intergenic | 30.0% |
| AGAGAGGACAAATGTGTTTA+TGG | + | Chr2:7466802-7466821 | MsG0280006874.01.T01:CDS | 35.0% | |
| TTTAATAGCACAGATGGTGC+TGG | + | Chr2:7466993-7467012 | MsG0280006874.01.T01:intron | 40.0% | |
| AGTCGATGCAGTGATGAAGA+CGG | + | Chr2:7467041-7467060 | MsG0280006874.01.T01:CDS | 45.0% | |
| CAGTGATGAAGACGGAAGAG+AGG | + | Chr2:7467049-7467068 | MsG0280006874.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 7466788 | 7467133 | 7466788 | ID=MsG0280006874.01;Name=MsG0280006874.01 |
| Chr2 | mRNA | 7466788 | 7467133 | 7466788 | ID=MsG0280006874.01.T01;Parent=MsG0280006874.01;Name=MsG0280006874.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|0|0.5|2|0|81 |
| Chr2 | exon | 7466788 | 7466898 | 7466788 | ID=MsG0280006874.01.T01:exon:7881;Parent=MsG0280006874.01.T01 |
| Chr2 | exon | 7466999 | 7467133 | 7466999 | ID=MsG0280006874.01.T01:exon:7882;Parent=MsG0280006874.01.T01 |
| Chr2 | CDS | 7466788 | 7466898 | 7466788 | ID=MsG0280006874.01.T01:cds;Parent=MsG0280006874.01.T01 |
| Chr2 | CDS | 7466999 | 7467133 | 7466999 | ID=MsG0280006874.01.T01:cds;Parent=MsG0280006874.01.T01 |
| Gene Sequence |
| Protein sequence |
